SUPPLEMENTARY DATA - Diabetes · plasma prekallikrein (PPK) (A) and FXII (B) in vitreous were...
6
SUPPLEMENTARY DATA ©2015 American Diabetes Association. Published online at http://diabetes.diabetesjournals.org/lookup/suppl/doi:10.2337/db15-0317/-/DC1 Supplementary Figure 1. Quantification of plasma KKS components in vitreous. Concentrations of plasma prekallikrein (PPK) (A) and FXII (B) in vitreous were quantified using a standard curve of band intensities of purified proteins visualized by western blotting. Controls showing PKal from normal human plasma and FXIIa from a mixture of PPK, FXII and HK were generated by incubation for 1 hr with kaolin (K) at room temperature (24°C) prior to gel loading. Western blot analyses of carboxypeptidase N (CPN) and hemoglobin in vitreous (C). Representative vitreous samples for patients with macular hole (MH), diabetic macular edema (DME), and proliferative diabetic retinopathy (PDR) are shown.
Transcript of SUPPLEMENTARY DATA - Diabetes · plasma prekallikrein (PPK) (A) and FXII (B) in vitreous were...
SUPPLEMENTARY DATA
©2015 American Diabetes Association. Published online at http://diabetes.diabetesjournals.org/lookup/suppl/doi:10.2337/db15-0317/-/DC1
Supplementary Figure 1. Quantification of plasma KKS components in vitreous. Concentrations of plasma prekallikrein (PPK) (A) and FXII (B) in vitreous were quantified using a standard curve of band intensities of purified proteins visualized by western blotting. Controls showing PKal from normal human plasma and FXIIa from a mixture of PPK, FXII and HK were generated by incubation for 1 hr with kaolin (K) at room temperature (24°C) prior to gel loading. Western blot analyses of carboxypeptidase N (CPN) and hemoglobin in vitreous (C). Representative vitreous samples for patients with macular hole (MH), diabetic macular edema (DME), and proliferative diabetic retinopathy (PDR) are shown.
SUPPLEMENTARY DATA
©2015 American Diabetes Association. Published online at http://diabetes.diabetesjournals.org/lookup/suppl/doi:10.2337/db15-0317/-/DC1
Supplementary Figure 2. Comparisons among KKS components and VEGF in DME vitreous. Concentration correlations for PPK and HK (A), PPK and FXII (B), FXII and HK (C), FXII and VEGF (D), and HK and VEGF (E).
SUPPLEMENTARY DATA
©2015 American Diabetes Association. Published online at http://diabetes.diabetesjournals.org/lookup/suppl/doi:10.2337/db15-0317/-/DC1
Supplementary Figure 3. B1R and B2R levels in retina from diabetic (DM) and nondiabetic (NDM) rats, and bovine retinal endothelial cells (BREC) and rat astrocytes exposed to high glucose. (A) Total retinal protein was extracted from 4 weeks STZ-induced diabetic rats (DM, n=4) and age-matched non-diabetic controls (NDM, n=3), and analyzed by Western blotting. Total cell lysate was extracted from BRECs (n=3) (B) and astrocytes (n=3) (C), which were untreated or treated with media containing high glucose (25mM) for the indicated period. B1R and B2R levels were normalized to GAPDH levels. Signal intensities were quantified as percentages of B1R/GAPDH or B2R/GAPDH ratio compared with NDM or day 0 (untreated). **p<0.01, compared with NDM or day 0.
SUPPLEMENTARY DATA
©2015 American Diabetes Association. Published online at http://diabetes.diabetesjournals.org/lookup/suppl/doi:10.2337/db15-0317/-/DC1
Supplementary Figure 4. Blood glucose and body weight in diabetic (DM) and nondiabetic (NDM) wild type (WT) and Klkb1-/- mice. Blood glucose of female (A) and male (B) WT and Klkb1-/- mice at DM onset and 3 months post induction of diabetes and age-matched NDM controls. Body weight of female (C) and male (D) NDM and DM WT and Klkb1-/- mice at baseline and after 3 months of diabetes. For body weight * p<0.001 compared to baseline and ** p=0.001 NDM 3 month body weight compared to DM mice. For blood glucose * P<0.01 DM onset blood glucose compared to NDM mice and ** p<0.001 DM 3 month blood glucose compared to NDM mice.
SUPPLEMENTARY DATA
©2015 American Diabetes Association. Published online at http://diabetes.diabetesjournals.org/lookup/suppl/doi:10.2337/db15-0317/-/DC1
Supplementary Table 1.
SUPPLEMENTARY DATA
©2015 American Diabetes Association. Published online at http://diabetes.diabetesjournals.org/lookup/suppl/doi:10.2337/db15-0317/-/DC1
Supplementary Table 2.
