SUPPLEMENTAL INFORMATION

Preparation of artificial diet for rearing Beet Armyworm (Spodoptera exigua)

NOTE: Details in this procedure refer to preparation of 10L of diet. Adjust amounts accordingly to prepare alternate

amount (Table 1).

The best way to prepare the diet, and control temperatures is by using a steam jacketed mixing kettle, such as this one:

http://www.insectrearing.com/products/dpmixk.html

1. Weigh and gather the proper amounts of all ingredients before starting the kettle (see table below).

a. All ingredients can be set out uncovered at room temperature except for the Aureomycin, which must be

covered. Aureomycin becomes dried out and hard when exposed to air.

b. For Vitamin Mix, cholesterol, methyl paraben, sodium benzoate, sorbic acid, and aureomycin

i. Place household sieve over large cup

ii. Use the sieve to break up clumps and to facilitate mixing.

iii. Add the appropriate amounts of vitamin mix, cholesterol, methyl paraben, sodium benzoate, sorbic

acid, and aureomycin to deli cup

2. Pour 3500 mL of Milli-Q water into the Kettle.

3. Turn the dial on the kettle to “10” and place the thermometer in the water.

4. While the water is heating up, place 180 g of Agar into a 2 L beaker

5. Add 250 mL Milli-Q water and stir with spatula

6. Add another 250 mL Milli-Q water

7. Continue adding water until 1000 mL of Milli-Q water is in the beaker and keep stirring until all of the powder is wet

and mixed.

a. Note: The water added to the agar keeps the agar suspended in liquid and prevents clumping. Actual

amount of water needed to do so will vary and is not important. However it is important to not exceed the

overall amount of water for the diet amount prepared (i.e. 8500 mL for 10 L).

b. Note: DO NOT add the agar directly to the kettle and attempt to bring to a boil. Doing so will create a hard

crust of burnt agar along the bottom and the kettle will shut off without bringing the solution to a boil.

8. Once the kettle temperature reaches about 85C, turn on the mixer

a. Note: Throughout the rest of the procedure, have the mixer set to the highest speed possible that does not

splash

9. Add the agar solution to the kettle. Take a small portion of water from the remaining Milli-Q water (approximately

500 mL) and rinse the contents of the 2 L beaker into the kettle.

10. Continue to heat the solution for about 1 minute after it begins to foam and boil.If the solution is not allowed to boil

long enough, the agar will not dissolve properly and the diet with not solidify when cooled.

11. Turn the kettle dial to off. Add the remaining Milli-Q water (~ 3500 mL) to the kettle slowly.

a. Allow the temperature to equalize before proceeding. There is often a large initial drop in temperature, but

it quickly rises again.

12. Once the temperature of the solution cools to 75C begin to slowly add the ingredients listed in table 1, one by one.

13. Pause periodically to allow the powder to mix into the solution

14. Once the temperature of the solution has cooled to 70C begin to slowly add the remaining ingredients one at a

time in the order listed. The sieved mixture should be added last.

a. Again, pause between each one to allow them to thoroughly mix into the solution.

15. Turn kettle back on and place dial on #5. Temperature of diet should be around 60°C.

16. Place the diet into desired containers, allow to solidify and store at 4°C until ready to use.

Table 1. Amount of diet can make and corresponding amount of ingredients to use.

BAW KETTLE DIET TABLE

4L 6L 8L 10L 12L

Total amount Milli-Q water 3400 mL 5100 mL 6800 mL 8500 mL 10200 mL

Milli-Q water (into kettle) 1400 mL 2800 mL 3000 mL 3500 mL 5500 mL

Kettle at 85°C

Milli-Q Water (beaker) 400 mL 750 mL 800mL 1000mL 6000mL

Agar (beaker) 72g 108g 144g 180g 216g

Milli-Q Water (rinse beaker) 200 mL 300 mL 400 mL 500 mL 600 mL

After Boiling

Milli-Q Water 1400mL 2100mL 2800mL 3500mL 4200mL

75°C or Less

Cellulose 40g 60g 80g 100g 120g

Sucrose 90g 135g 180g 225g 270g

Casein 120g 180g 240g 300g 360g

Wheat germ 180g 270g 360g 450g 540g

Wesson Salt Mix 38g 57g 76g 95g 114g

70°C or Less

Milli-Q Water 6.8mL 10.2mL 13.6mL 17mL 20.4mL

Ethyl-Alcohol 20mL 30mL 40mL 50mL 60mL

P/P Acid 10mL 15mL 20mL 25mL 30mL

Linseed Oil 6.8mL 10.2mL 13.6mL 17mL 20.4mL

USDA Vitamin Mix 60g 90g 120g 150g 180g

Cholesterol 0.2g 0.3g 0.4g 0.5g 0.6g

Methyl Paraben 4g 6g 8g 10g 12g

Sodium Benzoate 1.2g 1.8g 2.4g 3g 3.6g

Sorbic Acid 4g 6g 8g 10g 12g

Aureomycin 3.6g 5.4g 7.2g 9g 10.8g

Results of Biolog Phenotypic Microarray

BsppSIDx6186xA396

BiOLOG

21124 Cabot Blvd.

Hayward, CA 94545

Phenotype MicroArray Analysis

Test: Bspp_SID_6186_A396

Reference: none

Report Date: 10/10/2011 1:50:14 PM

Reproducibiltiy Analysis:

Test Reference

PM01: 00: passes PM01: -1: N/A

PM02: 00: passes PM02: -1: N/A

PM03: 00: passes PM03: -1: N/A

PM04: 00: passes PM04: -1: N/A

PM05: 00: passes PM05: -1: N/A

PM06: 00: passes PM06: -1: N/A

PM07: 00: passes PM07: -1: N/A

PM08: 00: passes PM08: -1: N/A

PM09: 00: passes PM09: -1: N/A

PM10: 05: passes PM10: -1: N/A

PM11: 00: passes PM11: -1: N/A

PM12: 00: passes PM12: -1: N/A

PM13: 00: passes PM13: -1: N/A

PM14: 00: passes PM14: -1: N/A

PM15: 00: passes PM15: -1: N/A

PM16: 00: passes PM16: -1: N/A

PM17: 00: passes PM17: -1: N/A

PM18: 01: passes PM18: -1: N/A

PM19: 00: passes PM19: -1: N/A

PM20: 04: passes PM20: -1: N/A

PM21: -1: N/A PM21: -1: N/A

PM22: -1: N/A PM22: -1: N/A

PM23: -1: N/A PM23: -1: N/A

PM24: -1: N/A PM24: -1: N/A

PM25: -1: N/A PM25: -1: N/A

PM26: -1: N/A PM26: -1: N/A

PM27: -1: N/A PM27: -1: N/A

PM28: -1: N/A PM28: -1: N/A

PM29: -1: N/A PM29: -1: N/A

PM30: -1: N/A PM30: -1: N/A

PM31: -1: N/A PM31: -1: N/A

PM32: -1: N/A PM32: -1: N/A

PM33: -1: N/A PM33: -1: N/A

PM34: -1: N/A PM34: -1: N/A

PM35: -1: N/A PM35: -1: N/A

PM36: -1: N/A PM36: -1: N/A

PM37: -1: N/A PM37: -1: N/A

PM38: -1: N/A PM38: -1: N/A

PM39: -1: N/A PM39: -1: N/A

PM40: -1: N/A PM40: -1: N/A

Replicate 1 versus Replicate 2 of Test:

Bspp_SID_6186_A396

Run 1:

Bspp_SID_6186_A396

Run 2:

Bspp_SID_6186_A396

Consensus:

Bspp_SID_6186_A396

PM Report:

Bspp_SID_6186_A396_

Phenotypes Gained:

PM20B C08 12 Atropine acetylcholine receptor, antagonist

PM18C F03,F04 12 Semicarbazide hydrochloride amine oxidase inhibitor, carcinogen

PM20B B04 36 Orphenadrine anti-cholinergic

PM14A A11 41 Sanguinarine chloride ATPase, Na+/K+ and Mg++

PM17A B03,B04 60 Salicylate, sodium biofilm inhibitor, anti-capsule agent, chelator,

prostaglandin syntetase inhibitor, mar inducer

PM02A H09 71 Dihydroxyacetone C-Source, alcohol

PM01 B12 96 L-Glutamic acid C-Source, amino acid

PM01 E01 86 L-Glutamine C-Source, amino acid

PM01 A08 79 L-Proline C-Source, amino acid

PM01 C01 89 D-Glucose-6-Phosphate C-Source, carbohydrate

PM01 E04 79 D-Fructose-6-Phosphate C-Source, carbohydrate

PM01 C09 76 a-D-Glucose C-Source, carbohydrate

PM01 A10 61 D-Trehalose C-Source, carbohydrate

PM01 B11 61 D-Mannitol C-Source, carbohydrate

PM02A D10 129 g-Amino-N-Butyric acid C-Source, carboxylic acid

PM01 G12 71 L-Malic acid C-Source, carboxylic acid

PM01 H08 71 Pyruvic acid C-Source, carboxylic acid

PM02A E12 69 5-Keto-D-Gluconic acid C-Source, carboxylic acid

PM02A E01 66 Capric acid C-Source, carboxylic acid

PM02A D12 64 Butyric acid C-Source, carboxylic acid

PM02A E02 64 Caproic acid C-Source, carboxylic acid

PM15B B08 35 EDTA chelator, hydrophilic

PM15B C10,C11,C12 75 1,10- Phenanthroline Monohydrate chelator, lipophilic

PM16A A12 42 5-Chloro-7-iodo-8-hydroxy-quinoline chelator, lipophilic

PM14A B08 15 Fusaric acid chelator, lipophilic

PM15B C04 9 5,7- Dichloro-8-hydroxyquinoline chelator, lipophilic

PM17A H07,H08 60 Caffeine cyclic AMP phosphodiesterase inhibitor

PM19 G12 36 Hydroxylamine DNA damage, mutagen, antifolate (inhibits thymine

and methionine synthesis)

PM19 C07,C08 53 7-Hydroxycoumarin DNA intercalator

PM19 A11 25 Coumarin DNA intercalator

PM20B F04 17 4-Hydroxycoumarin DNA intercalator

PM19 H02,H03,H04 75 Hexammine cobalt (III) chloride DNA synthesis

PM12B D09,D10,D11 82 Novobiocin DNA topoisomerase

PM11C E12 40 Nalidixic acid DNA topoisomerase

PM12B E08 40 Sulfadiazine folate antagonist, PABA analog

PM18C C08 28 Sulfisoxazole folate antagonist, PABA analog

PM16A H07,H08 26 4-Chloro-3,5-dimethyl-phenol fungicide

PM20B G02,G03,G04 54 Captan fungicide, carbamate

PM16A C04 30 Dichlofluanid fungicide, phenylsulphamide

PM20B H07 21 Tolylfluanid fungicide, phenylsulphamide

PM19 B08 9 Harmane imidazoline binding sites, agonist

PM17A A03,A04 36 D-Serine inhibits 3PGA dehydrogenase (L-serine and

pantothenate synthesis)

PM17A D06,D07,D08 90 3-Amino-1,2,4-triazole inhibits catalase, inhibits histidine synthesis

PM17A C04 13 4-Aminopyridine ion channel inhibitor, K+

PM18C D11 30 Lidocaine ion channel inhibitor, Na+, anaesthetic

PM20B E08 45 Dodine (n-Dodecylguanidine) membrane permeability, guanidine, fungicide

PM15B A06,A07,A08 41 Guanidine hydrochloride membrane, chaotropic agent

PM17A E04 4 Niaproof membrane, detergent, anionic

PM12B E12 40 Benzethonium Chloride membrane, detergent, cationic

PM19 B03,B04 37 Methyltrioctylammonium chloride membrane, detergent, cationic

PM15B D08 42 Domiphen bromide membrane, detergent, cationic, fungicide

PM19 G02,G03,G04 81 Lauryl sulfobetaine (N-Dodecyl-N,N-dimethyl-3- ammonio-1-propanesulfonate)

membrane, detergent, zwitterionic

PM14A H08 45 Promethazine membrane, phenothiazine, efflux pump inhibitor,

anti-psychotic

PM08 A02 204 L-Glutamine N-Source, amino acid

PM06 A02 191 L-Glutamine N-Source, amino acid

PM07 A02 188 L-Glutamine N-Source, amino acid

PM03B B01 185 L-Glutamine N-Source, amino acid

PM03B B09 185 L-Proline N-Source, amino acid

PM03B B03 168 L-Histidine N-Source, amino acid

PM03B A09 154 L-Asparagine N-Source, amino acid

PM03B B10 153 L-Serine N-Source, amino acid

PM03B A12 143 L-Glutamic acid N-Source, amino acid

PM03B D03 143 L-Pyroglutamic acid N-Source, amino acid

PM03B B11 140 L-Threonine N-Source, amino acid

PM03B A07 138 L-Alanine N-Source, amino acid

PM03B B06 138 L-Lysine N-Source, amino acid

PM03B B08 138 L-Phenylalanine N-Source, amino acid

PM03B C03 133 D-Alanine N-Source, amino acid

PM03B A08 122 L-Arginine N-Source, amino acid

PM03B B02 121 Glycine N-Source, amino acid

PM03B C01 120 L-Tyrosine N-Source, amino acid

PM03B A10 119 L-Aspartic acid N-Source, amino acid

PM03B C02 114 L-Valine N-Source, amino acid

PM03B C06 90 D-Glutamic acid N-Source, amino acid

PM03B C11 81 L-Homoserine N-Source, amino acid

PM03B B04 76 L-Isoleucine N-Source, amino acid

PM03B C04 70 D-Asparagine N-Source, amino acid

PM03B B12 66 L-Tryptophan N-Source, amino acid

PM03B C08 61 D-Serine N-Source, amino acid

PM03B A02 178 Ammonia N-Source, inorganic

PM03B A03 88 Nitrite N-Source, inorganic

PM03B A04 62 Nitrate N-Source, inorganic

PM03B G08 185 g-Amino-N-Butyric acid N-Source, other

PM03B F03 140 Adenosine N-Source, other

PM03B F12 136 Inosine N-Source, other

PM03B G02 136 Xanthosine N-Source, other

PM03B F07 135 Guanosine N-Source, other

PM03B G01 129 Xanthine N-Source, other

PM03B G03 124 Uric acid N-Source, other

PM03B G05 122 Allantoin N-Source, other

PM03B A05 109 Urea N-Source, other

PM03B E11 107 N-Acetyl-D-Glucosamine N-Source, other

PM03B D11 106 Putrescine N-Source, other

PM03B E02 106 b-Phenylethylamine N-Source, other

PM03B F05 102 Cytosine N-Source, other

PM03B G09 86 e-Amino-N-Caproic acid N-Source, other

PM03B D09 85 Ethanolamine N-Source, other

PM03B F02 81 Adenine N-Source, other

PM03B G06 77 Parabanic acid N-Source, other

PM03B F10 76 Uracil N-Source, other

PM03B D12 71 Agmatine N-Source, other

PM03B G12 69 a-Amino-N-Valeric acid N-Source, other

PM03B F09 65 Thymidine N-Source, other

PM03B F08 60 Thymine N-Source, other

PM03B H02 198 Ala-Gln N-Source, peptide

PM08 D12 195 Thr-Gln N-Source, peptide

PM03B H09 188 Gly-Gln N-Source, peptide

PM06 D01 186 Asp-Phe N-Source, peptide

PM08 A04 183 Ala-Gln N-Source, peptide

PM03B H05 182 Ala-His N-Source, peptide

PM06 C02 178 Arg-Phe N-Source, peptide

PM06 D05 175 Gln-Gln N-Source, peptide

PM07 E03 174 Ser-His N-Source, peptide

PM08 A09 174 Asp-Gln N-Source, peptide

PM06 C01 171 Arg-Met N-Source, peptide

PM08 D09 170 Ser-Gln N-Source, peptide

PM06 A08 169 Ala-His N-Source, peptide

PM08 E01 168 Thr-Phe N-Source, peptide

PM08 B03 167 His-Ala N-Source, peptide

PM08 H11 167 Phe-Gly-Gly N-Source, peptide

PM03B H08 166 Gly-Asn N-Source, peptide

PM06 A11 165 Ala-Phe N-Source, peptide

PM08 F06 165 b-Ala-Phe N-Source, peptide

PM06 A04 164 Ala-Arg N-Source, peptide

PM07 C09 163 Phe-Ala N-Source, peptide

PM08 B08 163 Leu-Asn N-Source, peptide

PM07 E06 162 Ser-Phe N-Source, peptide

PM06 A05 160 Ala-Asn N-Source, peptide

PM06 B05 160 Arg-Ala N-Source, peptide

PM06 B11 160 Arg-Leu N-Source, peptide

PM08 C11 160 Pro-Arg N-Source, peptide

PM03B H07 159 Ala-Thr N-Source, peptide

PM07 D02 158 Phe-Ser N-Source, peptide

PM08 C01 158 Lys-Gly N-Source, peptide

PM08 G11 158 Ala-Ala-Ala N-Source, peptide

PM06 H05 157 Leu-Arg N-Source, peptide

PM07 H04 157 Val-Asn N-Source, peptide

PM08 H06 157 Gly-Gly-Phe N-Source, peptide

PM06 D06 156 Gln-Gly N-Source, peptide

PM08 D07 156 Ser-Asn N-Source, peptide

PM08 E07 156 Val-Gln N-Source, peptide

PM08 B09 155 Leu-His N-Source, peptide

PM08 F07 155 D-Ala-D-Ala N-Source, peptide

PM06 A03 154 Ala-Ala N-Source, peptide

PM06 B01 154 Ala-Ser N-Source, peptide

PM07 E12 154 Thr-Arg N-Source, peptide

PM06 B06 153 Arg-Arg N-Source, peptide

PM06 F07 153 His-Leu N-Source, peptide

PM08 E02 153 Thr-Ser N-Source, peptide

PM03B H06 152 Ala-Leu N-Source, peptide

PM06 E10 152 Gly-Phe N-Source, peptide

PM07 B03 152 Lys-Thr N-Source, peptide

PM08 A08 152 Asp-Ala N-Source, peptide

PM06 B08 151 Arg-Gln N-Source, peptide

PM06 C05 151 Arg-Tyr N-Source, peptide

PM07 A06 151 Lys-Ala N-Source, peptide

PM07 C10 151 Phe-Gly N-Source, peptide

PM06 A09 150 Ala-Leu N-Source, peptide

PM08 C02 150 Lys-Met N-Source, peptide

PM06 A10 149 Ala-Lys N-Source, peptide

PM06 B04 149 Ala-Tyr N-Source, peptide

PM07 B02 149 Lys-Ser N-Source, peptide

PM06 F11 148 His-Ser N-Source, peptide

PM07 E11 148 Thr-Ala N-Source, peptide

PM08 G01 148 g-Glu-Gly N-Source, peptide

PM08 B05 147 His-His N-Source, peptide

PM06 C03 146 Arg-Ser N-Source, peptide

PM06 H04 146 Leu-Ala N-Source, peptide

PM07 E01 146 Ser-Ala N-Source, peptide

PM08 H09 145 Leu-Gly-Gly N-Source, peptide

PM08 H12 145 Tyr-Gly-Gly N-Source, peptide

PM03B H04 144 Ala-Gly N-Source, peptide

PM06 B02 144 Ala-Thr N-Source, peptide

PM06 B12 144 Arg-Lys N-Source, peptide

PM06 E06 144 Gly-His N-Source, peptide

PM07 F12 143 Trp-Lys N-Source, peptide

PM08 A12 143 Gly-Asn N-Source, peptide

PM07 A03 142 Leu-Ser N-Source, peptide

PM07 H03 142 Val-Arg N-Source, peptide

PM08 H01 142 Gly-Gly-Ala N-Source, peptide

PM06 H08 141 Leu-Gly N-Source, peptide

PM07 H12 141 g-Glu-Gly N-Source, peptide

PM08 C09 140 Phe-Tyr N-Source, peptide

PM06 G02 139 His-Val N-Source, peptide

PM07 A12 139 Lys-Phe N-Source, peptide

PM08 C08 139 Phe-Met N-Source, peptide

PM03B H03 138 Ala-Glu N-Source, peptide

PM06 C08 138 Asn-Val N-Source, peptide

PM07 A07 138 Lys-Arg N-Source, peptide

PM08 A05 138 Ala-Ile N-Source, peptide

PM06 F08 137 His-Lys N-Source, peptide

PM07 G09 137 Tyr-His N-Source, peptide

PM08 A07 137 Ala-Val N-Source, peptide

PM08 C12 137 Pro-Asn N-Source, peptide

PM08 E06 137 Val-Ala N-Source, peptide

PM06 F09 136 His-Met N-Source, peptide

PM06 C10 135 Asp-Glu N-Source, peptide

PM06 E03 135 Gly-Arg N-Source, peptide

PM06 G05 135 Ile-Gln N-Source, peptide

PM07 C12 135 Phe-Phe N-Source, peptide

PM07 G05 135 Tyr-Ala N-Source, peptide

PM08 C10 135 Phe-Val N-Source, peptide

PM08 E11 135 Val-Phe N-Source, peptide

PM08 H05 135 Gly-Gly-Leu N-Source, peptide

PM03B H12 134 Met-Ala N-Source, peptide

PM07 E04 134 Ser-Leu N-Source, peptide

PM08 F01 134 Val-Ser N-Source, peptide

PM08 F04 133 b-Ala-His N-Source, peptide

PM06 B10 132 Arg-Ile N-Source, peptide

PM06 F06 132 His-Gly N-Source, peptide

PM07 F03 132 Thr-Leu N-Source, peptide

PM07 F06 132 Trp-Ala N-Source, peptide

PM07 G06 132 Tyr-Gln N-Source, peptide

PM06 D03 131 Asp-Val N-Source, peptide

PM07 B12 131 Met-His N-Source, peptide

PM07 G02 131 Trp-Ser N-Source, peptide

PM08 C07 131 Gln-Glu N-Source, peptide

PM06 E02 130 Gly-Ala N-Source, peptide

PM07 B09 130 Met-Gln N-Source, peptide

PM07 E09 130 Ser-Tyr N-Source, peptide

PM07 F07 130 Trp-Arg N-Source, peptide

PM08 B06 130 Ile-Asn N-Source, peptide

PM06 F03 129 Gly-Tyr N-Source, peptide

PM07 B05 129 Lys-Tyr N-Source, peptide

PM08 B04 129 His-Glu N-Source, peptide

PM08 F03 129 b-Ala-Gly N-Source, peptide

PM07 C03 128 Met-Lys N-Source, peptide

PM06 C06 127 Arg-Val N-Source, peptide

PM06 G01 127 His-Tyr N-Source, peptide

PM07 E08 127 Ser-Ser N-Source, peptide

PM07 G11 127 Tyr-Lys N-Source, peptide

PM06 F10 126 His-Pro N-Source, peptide

PM06 D02 125 Asp-Trp N-Source, peptide

PM07 A09 125 Lys-Ile N-Source, peptide

PM07 G08 125 Tyr-Gly N-Source, peptide

PM06 E12 124 Gly-Ser N-Source, peptide

PM07 A11 124 Lys-Lys N-Source, peptide

PM08 B02 123 Gly-Ile N-Source, peptide

PM08 B11 123 Leu-Tyr N-Source, peptide

PM08 H08 123 Gly-Phe-Phe N-Source, peptide

PM06 C04 122 Arg-Trp N-Source, peptide

PM06 C12 122 Asp-Lys N-Source, peptide

PM07 B07 122 Met-Arg N-Source, peptide

PM08 E09 122 Val-Lys N-Source, peptide

PM07 E10 121 Ser-Val N-Source, peptide

PM07 H07 121 Val-His N-Source, peptide

PM08 A06 121 Ala-Met N-Source, peptide

PM07 B06 120 Lys-Val N-Source, peptide

PM07 C11 120 Phe-Ile N-Source, peptide

PM06 A06 119 Ala-Glu N-Source, peptide

PM06 B03 119 Ala-Trp N-Source, peptide

PM06 E07 118 Gly-Leu N-Source, peptide

PM06 A07 117 Ala-Gly N-Source, peptide

PM07 G12 117 Tyr-Phe N-Source, peptide

PM07 G04 116 Trp-Tyr N-Source, peptide

PM06 G04 113 Ile-Arg N-Source, peptide

PM07 D10 113 Pro-Phe N-Source, peptide

PM07 A10 112 Lys-Leu N-Source, peptide

PM08 C03 112 Met-Thr N-Source, peptide

PM07 B04 109 Lys-Trp N-Source, peptide

PM03B H11 108 Gly-Met N-Source, peptide

PM07 H10 108 Val-Tyr N-Source, peptide

PM08 A03 107 Ala-Asp N-Source, peptide

PM06 E08 106 Gly-Lys N-Source, peptide

PM07 F02 106 Thr-Gly N-Source, peptide

PM07 F10 106 Trp-Gly N-Source, peptide

PM06 F01 105 Gly-Thr N-Source, peptide

PM06 G07 105 Ile-His N-Source, peptide

PM06 H12 105 Leu-Phe N-Source, peptide

PM07 H06 103 Val-Gly N-Source, peptide

PM06 C11 102 Asp-Leu N-Source, peptide

PM06 F04 102 Gly-Val N-Source, peptide

PM07 E02 102 Ser-Gly N-Source, peptide

PM08 A10 102 Asp-Gly N-Source, peptide

PM06 B09 101 Arg-Glu N-Source, peptide

PM07 E05 101 Ser-Met N-Source, peptide

PM08 A11 101 Glu-Ala N-Source, peptide

PM06 C07 100 Asn-Glu N-Source, peptide

PM08 C04 99 Met-Tyr N-Source, peptide

PM03B H10 98 Gly-Glu N-Source, peptide

PM06 E05 98 Gly-Gly N-Source, peptide

PM07 B11 98 Met-Gly N-Source, peptide

PM07 F04 98 Thr-Met N-Source, peptide

PM08 H04 98 Gly-Gly-Ile N-Source, peptide

PM06 G03 97 Ile-Ala N-Source, peptide

PM06 E09 96 Gly-Met N-Source, peptide

PM08 E04 96 Tyr-Ile N-Source, peptide

PM07 F11 95 Trp-Leu N-Source, peptide

PM07 A08 94 Lys-Glu N-Source, peptide

PM07 H09 94 Val-Leu N-Source, peptide

PM08 B07 93 Ile-Leu N-Source, peptide

PM06 D04 91 Cys-Gly N-Source, peptide

PM06 G12 91 Ile-Ser N-Source, peptide

PM07 A04 91 Leu-Trp N-Source, peptide

PM08 B12 91 Lys-Asp N-Source, peptide

PM06 E11 89 Gly-Pro N-Source, peptide

PM07 A05 88 Leu-Val N-Source, peptide

PM07 C05 88 Met-Phe N-Source, peptide

PM07 D12 87 Pro-Tyr N-Source, peptide

PM07 H11 87 Val-Val N-Source, peptide

PM06 B07 85 Arg-Asp N-Source, peptide

PM07 G01 84 Trp-Phe N-Source, peptide

PM08 D06 84 Pro-Val N-Source, peptide

PM06 A12 83 Ala-Pro N-Source, peptide

PM06 F02 83 Gly-Trp N-Source, peptide

PM08 E05 83 Tyr-Val N-Source, peptide

PM06 C09 82 Asp-Asp N-Source, peptide

PM06 H07 81 Leu-Glu N-Source, peptide

PM08 D03 81 Pro-Lys N-Source, peptide

PM07 G03 80 Trp-Trp N-Source, peptide

PM06 E04 79 Gly-Cys N-Source, peptide

PM07 G10 79 Tyr-Leu N-Source, peptide

PM08 E08 79 Val-Glu N-Source, peptide

PM08 G08 79 Leu-b-Ala N-Source, peptide

PM08 B10 76 Leu-Pro N-Source, peptide

PM08 D04 76 Pro-Ser N-Source, peptide

PM06 D07 75 Glu-Asp N-Source, peptide

PM06 G06 75 Ile-Gly N-Source, peptide

PM07 D03 75 Phe-Trp N-Source, peptide

PM08 H03 75 Gly-Gly-Gly N-Source, peptide

PM07 H02 74 Tyr-Tyr N-Source, peptide

PM06 G10 70 Ile-Phe N-Source, peptide

PM07 H08 70 Val-Ile N-Source, peptide

PM08 G03 70 Gly-D-Ala N-Source, peptide

PM07 D06 67 Pro-Gln N-Source, peptide

PM08 F02 67 b-Ala-Ala N-Source, peptide

PM08 E12 66 Val-Pro N-Source, peptide

PM08 C06 65 Phe-Glu N-Source, peptide

PM07 D04 64 Pro-Ala N-Source, peptide

PM08 D08 64 Ser-Asp N-Source, peptide

PM08 E10 63 Val-Met N-Source, peptide

PM15B H03,H04 54 2- Nitroimidazole nitro compound, oxidizing agent, ribonucleotide DP

reductase inhibitor

PM12B F10,F11,F12 94 5-Fluoroorotic acid nucleic acid analog, pyrimidine

PM05 H09 169 Tween 20 Nutritional supplement

PM05 H02 165 a-Hydroxybutyric acid Nutritional supplement

PM05 H11 164 Tween 60 Nutritional supplement

PM05 G01 161 Oxaloacetic acid Nutritional supplement

PM05 C01 159 L-Proline Nutritional supplement

PM05 E12 159 2`-Deoxyuridine Nutritional supplement

PM05 G12 159 m-Inositol Nutritional supplement

PM05 H12 159 Tween 80 Nutritional supplement

PM05 F12 158 Thymidine Nutritional supplement

PM05 D03 157 Chorismic acid Nutritional supplement

PM05 F11 157 Glutathione Nutritional supplement

PM05 H03 157 a-Ketobutyric acid Nutritional supplement

PM05 F01 155 Quinolinic acid Nutritional supplement

PM05 F02 155 Nicotinic acid Nutritional supplement

PM05 G02 155 D-Biotin Nutritional supplement

PM05 G04 155 p-Amino-Benzoic acid Nutritional supplement

PM05 H04 155 Caprylic acid Nutritional supplement

PM05 B10 154 Guanine Nutritional supplement

PM05 E02 154 Spermidine Nutritional supplement

PM05 E03 154 Spermine Nutritional supplement

PM05 F04 154 b-Nicotinamide Adenine Dinucleotide Nutritional supplement

PM05 H05 154 D,L-Thioctic acid Nutritional supplement

PM05 H08 154 Choline Nutritional supplement

PM05 B02 153 Glycine Nutritional supplement

PM05 B04 153 L-Isoleucine Nutritional supplement

PM05 B12 153 2`-Deoxyguanosine Nutritional supplement

PM05 D12 153 2`-Deoxycytidine Nutritional supplement

PM05 H07 153 D,L-Carnitine Nutritional supplement

PM05 A11 152 Adenosine Nutritional supplement

PM05 C02 152 L-Serine Nutritional supplement

PM05 D02 152 L-Citrulline Nutritional supplement

PM05 G03 152 Cyanocobalamin Nutritional supplement

PM05 G10 152 Pyrrolo-Quinoline Quinone Nutritional supplement

PM05 B01 151 L-Glutamine Nutritional supplement

PM05 C11 151 Inosine Nutritional supplement

PM05 C12 151 2`-Deoxyinosine Nutritional supplement

PM05 D01 151 L-Ornithine Nutritional supplement

PM05 F03 151 Nicotinamide Nutritional supplement

PM05 F05 151 d-Amino-levulinic acid Nutritional supplement

PM05 G06 151 Inosine + Thiamine Nutritional supplement

PM05 G11 151 Menadione Nutritional supplement

PM05 H10 151 Tween 40 Nutritional supplement

PM05 E01 150 Putrescine Nutritional supplement

PM05 H06 150 D,L-Mevalonic acid Lactone Nutritional supplement

PM05 A10 149 Adenine Nutritional supplement

PM05 C04 149 L-Tryptophan Nutritional supplement

PM05 C10 149 Hypoxanthine Nutritional supplement

PM05 D10 149 Cytosine Nutritional supplement

PM05 D11 149 Cytidine Nutritional supplement

PM05 E11 149 Uridine Nutritional supplement

PM05 F09 149 N-Acetyl-D-Glucosamine Nutritional supplement

PM05 F10 149 Thymine Nutritional supplement

PM05 G05 149 Folic acid Nutritional supplement

PM05 G08 149 Thiamine Pyrophosphate Nutritional supplement

PM05 G09 149 Riboflavin Nutritional supplement

PM05 A03 148 L-Alanine Nutritional supplement

PM05 A04 148 L-Arginine Nutritional supplement

PM05 B03 148 L-Histidine Nutritional supplement

PM05 D04 148 (-)Shikimic acid Nutritional supplement

PM05 E04 148 Pyridoxine Nutritional supplement

PM05 F08 148 a-D-Glucose Nutritional supplement

PM05 G07 148 Thiamine Nutritional supplement

PM05 B06 147 L-Lysine Nutritional supplement

PM05 B11 147 Guanosine Nutritional supplement

PM05 A09 146 Adenosine 3`,5`-Cyclic Monophosphate Nutritional supplement

PM05 B05 146 L-Leucine Nutritional supplement

PM05 B09 146 Guanosine 3`,5`-Cyclic Monophosphate Nutritional supplement

PM05 C03 146 L-Threonine Nutritional supplement

PM05 C07 146 L-Isoleucine + L-Valine Nutritional supplement

PM05 D08 146 D-Glutamic acid Nutritional supplement

PM05 E10 146 Uracil Nutritional supplement

PM05 F07 146 Deferoxamine Nutritional supplement

PM05 A05 145 L-Asparagine Nutritional supplement

PM05 A06 145 L-Aspartic acid Nutritional supplement

PM05 B07 145 L-Methionine Nutritional supplement

PM05 C08 145 Hydroxy-L-Proline Nutritional supplement

PM05 D05 145 L-Homoserine Lactone Nutritional supplement

PM05 E08 145 D-Pantothenic acid Nutritional supplement

PM05 F06 145 Hematin Nutritional supplement

PM05 C05 144 L-Tyrosine Nutritional supplement

PM05 C06 144 L-Valine Nutritional supplement

PM05 D09 144 D,L-Diamino-a,e-Pimelic acid Nutritional supplement

PM05 E05 144 Pyridoxal Nutritional supplement

PM05 E06 144 Pyridoxamine Nutritional supplement

PM05 E09 144 Orotic acid Nutritional supplement

PM05 A07 143 L-Cysteine Nutritional supplement

PM05 D06 143 D-Alanine Nutritional supplement

PM05 E07 143 b-Alanine Nutritional supplement

PM05 H01 142 Butyric acid Nutritional supplement

PM05 B08 141 L-Phenylalanine Nutritional supplement

PM05 D07 141 D-Aspartic acid Nutritional supplement

PM05 A08 139 L-Glutamic acid Nutritional supplement

PM05 C09 138 (5) 4-Amino-Imidazole-4(5)-Carboxamide Nutritional supplement

PM05 A12 135 2`-Deoxyadenosine Nutritional supplement

PM05 A01 138 Negative Control Nutritional supplement, Negative control

PM05 A02 184 Positive Control Nutritional supplement, Positive control

PM09 C03 22 6% NaCl + N-Acetyl-L-Glutamine osmolyte, acetyl glutamine

PM09 C04 8 6% NaCl + b-Glutamic acid osmolyte, b-glutamate

PM09 B02 13 6% NaCl + Betaine osmolyte, betaine

PM09 B08 8 6% NaCl + Choline osmolyte, choline

PM09 B10 12 6% NaCl + Creatine osmolyte, creatine

PM09 B11 34 6% NaCl + Creatinine osmolyte, creatinine

PM09 B05 5 6% NaCl + Dimethyl Sulphonyl Propionate osmolyte, dimethyl sulphonyl

propionate

PM09 B03 11 6% NaCl + N,N Dimethyl Glycine osmolyte, dimethylglycine

PM09 B07 14 6% NaCl + Ectoine osmolyte, ectoine

PM09 C05 4 6% NaCl + g-Amino-N-Butyric acid osmolyte, g-amino butyric acid

PM09 C06 8 6% NaCl + Glutathione osmolyte, glutathione

PM09 C07 8 6% NaCl + Glycerol osmolyte, glycerol

PM09 C01 26 6% NaCl + KCl osmolyte, KCl

PM09 B06 26 6% NaCl + MOPS osmolyte, MOPS

PM09 C11 25 6% NaCl + Octopine osmolyte, octopine

PM09 B09 13 6% NaCl + Phosphorylcholine osmolyte, phosphorylcholine

PM09 C02 11 6% NaCl + L-Proline osmolyte, proline

PM09 B04 33 6% NaCl + Sarcosine osmolyte, sarcosine

PM09 C08 20 6% NaCl + Trehalose osmolyte, trehalose

PM09 C12 32 6% NaCl + Trigonelline osmolyte, trigonelline

PM09 C10 23 6% NaCl + Trimethylamine osmolyte, trimethylamine

PM09 C09 18 6% NaCl + Trimethylamine-N-Oxide osmolyte, trimethylamine-N-oxide

PM09 D01 20 3% Potassium Chloride osmotic sensitivity, KCl

PM09 D02 16 4% Potassium chloride osmotic sensitivity, KCl

PM09 D04 16 6% Potassium chloride osmotic sensitivity, KCl

PM09 D03 8 5% Potassium Chloride osmotic sensitivity, KCl

PM09 D06 32 3% Sodium Sulfate osmotic sensitivity, Na2SO4

PM09 D07 15 4% Sodium Sulfate osmotic sensitivity, Na2SO4

PM09 D08 10 5% Sodium Sulfate osmotic sensitivity, Na2SO4

PM09 A12 31 10% NaCl osmotic sensitivity, NaCl

PM09 A11 26 9% NaCl osmotic sensitivity, NaCl

PM09 A09 21 7% NaCl osmotic sensitivity, NaCl

PM09 A10 19 8% NaCl osmotic sensitivity, NaCl

PM09 A02 17 2% NaCl osmotic sensitivity, NaCl

PM09 A08 17 6.5% NaCl osmotic sensitivity, NaCl

PM09 A07 12 6% NaCl osmotic sensitivity, NaCl

PM09 A03 9 3% NaCl osmotic sensitivity, NaCl

PM09 A06 8 5.5 %NaCl osmotic sensitivity, NaCl

PM09 A04 7 4% NaCl osmotic sensitivity, NaCl

PM09 A05 7 5% NaCl osmotic sensitivity, NaCl

PM09 B01 22 6% NaCl osmotic sensitivity, NaCl control

PM09 E03 27 3% Sodium Formate osmotic sensitivity, sodium formate

PM09 E02 24 2% Sodium Formate osmotic sensitivity, sodium formate

PM09 E05 8 5% Sodium Formate osmotic sensitivity, sodium formate

PM09 E04 7 4% Sodium Formate osmotic sensitivity, sodium formate

PM09 E06 7 6% Sodium Formate osmotic sensitivity, sodium formate

PM09 F12 31 12% Sodium Lactate osmotic sensitivity, sodium lactate

PM09 F11 26 11% Sodium Lactate osmotic sensitivity, sodium lactate

PM09 F10 22 10% Sodium Lactate osmotic sensitivity, sodium lactate

PM09 F09 15 9% Sodium Lactate osmotic sensitivity, sodium lactate

PM09 F02 11 2% Sodium Lactate osmotic sensitivity, sodium lactate

PM09 F08 11 8% Sodium Lactate osmotic sensitivity, sodium lactate

PM09 F03 10 3% Sodium Lactate osmotic sensitivity, sodium lactate

PM09 F05 8 5% Sodium Lactate osmotic sensitivity, sodium lactate

PM09 F07 7 7% Sodium Lactate osmotic sensitivity, sodium lactate

PM09 F06 6 6% Sodium Lactate osmotic sensitivity, sodium lactate

PM09 F04 3 4% Sodium Lactate osmotic sensitivity, sodium lactate

PM09 E12 31 7% Urea osmotic sensitivity, urea

PM09 E11 23 6% Urea osmotic sensitivity, urea

PM09 E09 21 4% Urea osmotic sensitivity, urea

PM09 E10 17 5% Urea osmotic sensitivity, urea

PM14A D07,D08 57 Iodoacetic acid oxidizes sulfhydryls

PM16A D04 15 Chlorodinitrobenzene oxidizes sulfhydryls, depletes glutathione

PM15B F02,F03,F04 66 3,4- Dimethoxybenzyl alcohol oxidizing agent, free radical-peroxidase substrate

PM04A A05 158 Tripolyphosphate P-Source, inorganic

PM04A A02 152 Phosphate P-Source, inorganic

PM04A A03 152 Pyrophosphate P-Source, inorganic

PM04A B01 150 Thiophosphate P-Source, inorganic

PM04A A04 148 Trimetaphosphate P-Source, inorganic

PM04A B02 145 Dithiophosphate P-Source, inorganic

PM04A C07 162 6-Phospho-Gluconic acid P-Source, organic

PM04A C04 161 D-Glucose-6-Phosphate P-Source, organic

PM04A C06 161 D-Glucosamine-6-Phosphate P-Source, organic

PM04A D02 160 D-Mannose-6-Phosphate P-Source, organic

PM04A A10 158 Adenosine 5`-Monophosphate P-Source, organic

PM04A B07 158 D-3-Phospho-Glyceric acid P-Source, organic

PM04A E02 158 O-Phospho-L-Tyrosine P-Source, organic

PM04A B04 157 b-Glycerol Phosphate P-Source, organic

PM04A A08 156 Adenosine 2`-Monophosphate P-Source, organic

PM04A B03 156 D,L-a-Glycerol Phosphate P-Source, organic

PM04A C05 156 2-Deoxy-D-Glucose 6-Phosphate P-Source, organic

PM04A A09 155 Adenosine 3`-Monophosphate P-Source, organic

PM04A E10 154 Thymidine 5`-Monophosphate P-Source, organic

PM04A E04 153 Phosphoryl Choline P-Source, organic

PM04A B10 152 Guanosine 5`-Monophosphate P-Source, organic

PM04A C01 152 Phosphoenol Pyruvate P-Source, organic

PM04A D07 152 O-Phospho-L-Threonine P-Source, organic

PM04A A11 150 Adenosine 2`,3`-Cyclic Monophosphate P-Source, organic

PM04A B11 150 Guanosine 2`,3`-Cyclic Monophosphate P-Source, organic

PM04A C03 150 D-Glucose-1-Phosphate P-Source, organic

PM04A B09 149 Guanosine 3`-Monophosphate P-Source, organic

PM04A C09 149 Cytidine 3`-Monophosphate P-Source, organic

PM04A D04 148 Phospho-L-Arginine P-Source, organic

PM04A D06 148 O-Phospho-L-Serine P-Source, organic

PM04A D10 148 Uridine 5`-Monophosphate P-Source, organic

PM04A E01 148 O-Phospho-D-Tyrosine P-Source, organic

PM04A E03 147 Phosphocreatine P-Source, organic

PM04A B06 146 D-2-Phospho-Glyceric acid P-Source, organic

PM04A C10 146 Cytidine 5`-Monophosphate P-Source, organic

PM04A C12 146 Cytidine 3`,5`-Cyclic Monophosphate P-Source, organic

PM04A B05 145 Carbamyl Phosphate P-Source, organic

PM04A C11 145 Cytidine 2`,3`-Cyclic Monophosphate P-Source, organic

PM04A D09 145 Uridine 3`-Monophosphate P-Source, organic

PM04A E09 145 Thymidine 3`-Monophosphate P-Source, organic

PM04A B08 144 Guanosine 2`-Monophosphate P-Source, organic

PM04A B12 144 Guanosine 3`,5`-Cyclic Monophosphate P-Source, organic

PM04A A12 143 Adenosine 3`,5`-Cyclic Monophosphate P-Source, organic

PM04A C08 143 Cytidine 2`-Monophosphate P-Source, organic

PM04A D12 143 Uridine 3`,5`-Cyclic Monophosphate P-Source, organic

PM04A D08 142 Uridine 2`-Monophosphate P-Source, organic

PM04A E05 142 O-Phosphoryl-Ethanolamine P-Source, organic

PM04A D11 139 Uridine 2`,3`-Cyclic Monophosphate P-Source, organic

PM04A D05 136 O-Phospho-D-Serine P-Source, organic

PM04A E12 136 Thymidine 3`,5`-Cyclic Monophosphate P-Source, organic

PM04A C02 120 Phospho-Glycolic acid P-Source, organic

PM04A E07 116 2-Aminoethyl Phosphonic acid P-Source, organic

PM04A E11 92 Inositol Hexaphosphate P-Source, organic

PM04A D03 86 Cysteamine-S-Phosphate P-Source, organic

PM10 G11 34 pH 9.5 + Trimethylamine-N-Oxide pH, deaminase

PM10 G08 33 pH 9.5 + b-Phenylethylamine pH, deaminase

PM10 G12 30 pH 9.5 + Urea pH, deaminase

PM10 F06 28 pH 9.5 + L-Tryptophan pH, deaminase

PM10 E11 25 pH 9.5 + L-Leucine pH, deaminase

PM10 F12 25 pH 9.5 + L-Homoserine pH, deaminase

PM10 E08 19 pH 9.5 + Glycine pH, deaminase

PM10 F11 19 pH 9.5 + L-Homoarginine pH, deaminase

PM10 G01 19 pH 9.5 + Anthranilic acid pH, deaminase

PM10 G04 19 pH 9.5 + Agmatine pH, deaminase

PM10 G05 17 pH 9.5 + Cadaverine pH, deaminase

PM10 E10 16 pH 9.5 + L-Isoleucine pH, deaminase

PM10 F01 15 pH 9.5 + L-Methionine pH, deaminase

PM10 F09 15 pH 9.5 + Hydroxy-L-Proline pH, deaminase

PM10 E04 14 pH 9.5 + L-Asparagine pH, deaminase

PM10 F10 14 pH 9.5 + L-Ornithine pH, deaminase

PM10 E05 13 pH 9.5 + L-Aspartic acid pH, deaminase

PM10 E09 13 pH 9.5 + L-Histidine pH, deaminase

PM10 E06 11 pH 9.5 + L-Glutamic acid pH, deaminase

PM10 G02 11 pH 9.5 + L-Norleucine pH, deaminase

PM10 F02 10 pH 9.5 + L-Phenylalanine pH, deaminase

PM10 F08 10 pH 9.5 + L-Valine pH, deaminase

PM10 E02 8 pH 9.5 + L-Alanine pH, deaminase

PM10 E03 8 pH 9.5 + L-Arginine pH, deaminase

PM10 G03 8 pH 9.5 + L-Norvaline pH, deaminase

PM10 G07 8 pH 9.5 + Histamine pH, deaminase

PM10 F04 7 pH 9.5 + L-Serine pH, deaminase

PM10 F05 5 pH 9.5 + L-Threonine pH, deaminase

PM10 G06 3 pH 9.5 + Putrescine pH, deaminase

PM10 E01 19 pH 9.5 pH, deaminase control

PM10 D09 38 pH 4.5 + 5-Hydroxy-L-Tryptophan pH, decarboxylase

PM10 B11 33 pH 4.5 + L-Leucine pH, decarboxylase

PM10 B12 33 pH 4.5 + L-Lysine pH, decarboxylase

PM10 C12 30 pH 4.5 + L-Homoserine pH, decarboxylase

PM10 C11 28 pH 4.5 + L-Homoarginine pH, decarboxylase

PM10 D12 28 pH 4.5 + Urea pH, decarboxylase

PM10 C03 25 pH 4.5 + L-Proline pH, decarboxylase

PM10 B10 23 pH 4.5 + L-Isoleucine pH, decarboxylase

PM10 D11 22 pH 4.5 + Trimethylamine-N-Oxide pH, decarboxylase

PM10 D01 21 pH 4.5 + Anthranilic acid pH, decarboxylase

PM10 C09 19 pH 4.5 + Hydroxy-L-Proline pH, decarboxylase

PM10 D05 19 pH 4.5 + p-Aminobenzoate pH, decarboxylase

PM10 C10 18 pH 4.5 + L-Ornithine pH, decarboxylase

PM10 D06 18 pH 4.5 + L-Cysteic acid pH, decarboxylase

PM10 D10 18 pH 4.5 + D,L-Diamino-a,e-Pimelic acid pH, decarboxylase

PM10 C08 17 pH 4.5 + L-Valine pH, decarboxylase

PM10 D03 17 pH 4.5 + L-Norvaline pH, decarboxylase

PM10 C02 15 pH 4.5 + L-Phenylalanine pH, decarboxylase

PM10 B02 14 pH 4.5 + L-Alanine pH, decarboxylase

PM10 B07 14 pH 4.5 + L-Glutamine pH, decarboxylase

PM10 B08 13 pH 4.5 + Glycine pH, decarboxylase

PM10 B09 13 pH 4.5 + L-Histidine pH, decarboxylase

PM10 C04 13 pH 4.5 + L-Serine pH, decarboxylase

PM10 C05 13 pH 4.5 + L-Threonine pH, decarboxylase

PM10 B04 12 pH 4.5 + L-Asparagine pH, decarboxylase

PM10 B05 10 pH 4.5 + L-Aspartic acid pH, decarboxylase

PM10 C06 10 pH 4.5 + L-Tryptophan pH, decarboxylase

PM10 C07 10 pH 4.5 + L-Citrulline pH, decarboxylase

PM10 D04 10 pH 4.5 + a-Amino-N-Butyric acid pH, decarboxylase

PM10 B06 8 pH 4.5 + L-Glutamic acid pH, decarboxylase

PM10 D02 8 pH 4.5 + L-Norleucine pH, decarboxylase

PM10 B01 24 pH 4.5 pH, decarboxylase control

PM10 A12 37 pH 10 pH, growth at 10

PM10 A01 32 pH 3.5 pH, growth at 3.5

PM10 A02 25 pH 4 pH, growth at 4

PM13C B05,B06,B07,B08 50 Ribostamycin protein synthesis, 30S ribosomal subunit,

aminoglycoside

PM11C C10,C11,C12 73 Minocycline protein synthesis, 30S ribosomal subunit,

tetracycline

PM13C C07,C08 38 Doxycycline protein synthesis, 30S ribosomal subunit,

tetracycline

PM15B F07,F08 52 Oleandomycin, phosphate salt protein synthesis, 50S ribosomal subunit,

macrolide

PM19 A03,A04 43 Josamycin protein synthesis, 50S ribosomal subunit,

macrolide

PM13C H12 40 Tylosin protein synthesis, 50S ribosomal subunit,

macrolide

PM15B C06,C07,C08 37 Fusidic acid, sodium salt protein synthesis, elongation factor

PM19 E08 28 D,L-Thioctic acid Reducing agent

PM19 H07,H08 54 a-Monothioglycerol reducing agent, thiol, adenosyl methionine

antagonist

PM19 F11 35 Sodium Caprylate respiration, ionophore, H+

PM18C C11 34 Pentachlorophenol respiration, ionophore, H+

PM20B G08 11 3,5-Dinitrobenzoic acid respiration, ionophore, H+

PM15B G06,G07,G08 32 Sodium Azide respiration, uncoupler

PM15B G09 29 Menadione, sodium bisulfite respiration, uncoupler

PM17A D04 21 Oxycarboxin respiratory enzymes, carboxamide, fungicide

PM18C G07,G08 22 3,5- Diamino-1,2,4-triazole (Guanazole)ribonucleotide DP reductase inhibitor

PM15B H05,H06,H07,H08 70 Hydroxyurea ribonucleotide DP reductase inhibitor, antifolate

(inhibits thymine and methionine synthesis)

PM04A F02 149 Sulfate S-Source, inorganic

PM04A F03 149 Thiosulfate S-Source, inorganic

PM04A F04 146 Tetrathionate S-Source, inorganic

PM04A F06 132 Dithiophosphate S-Source, inorganic

PM04A F05 111 Thiophosphate S-Source, inorganic

PM04A G08 153 D-Methionine S-Source, organic

PM04A F07 150 L-Cysteine S-Source, organic

PM04A F12 149 L-Cysteine Sulfinic acid S-Source, organic

PM04A G03 149 Cystathionine S-Source, organic

PM04A G10 149 N-Acetyl-D,L-Methionine S-Source, organic

PM04A G07 148 L-Methionine S-Source, organic

PM04A G11 148 L-Methionine Sulfoxide S-Source, organic

PM04A G09 147 Gly-Met S-Source, organic

PM04A F09 146 Cys-Gly S-Source, organic

PM04A H10 145 2-Hydroxyethane Sulfonic acid S-Source, organic

PM04A H01 144 L-Djenkolic acid S-Source, organic

PM04A H09 144 Butane Sulfonic acid S-Source, organic

PM04A H11 141 Methane Sulfonic acid S-Source, organic

PM04A H06 139 Taurine S-Source, organic

PM04A G04 138 Lanthionine S-Source, organic

PM04A H07 138 Hypotaurine S-Source, organic

PM04A G12 128 L-Methionine Sulfone S-Source, organic

PM04A G02 122 S-Methyl-L-Cysteine S-Source, organic

PM04A G06 121 D,L-Ethionine S-Source, organic

PM04A F10 96 L-Cysteic acid S-Source, organic

PM04A F11 95 Cysteamine S-Source, organic

PM04A F08 89 D-Cysteine S-Source, organic

PM04A H02 87 Thiourea S-Source, organic

PM04A H04 77 D,L-Lipoamide S-Source, organic

PM14A C01,C02,C03,C04 90 Boric acid toxic anion

PM13C C09,C10,C11,C12 89 Potassium chromate toxic anion

PM14A E09,E10,E11 79 Sodium metaborate toxic anion

PM14A C11,C12 56 Sodium Cyanate toxic anion

PM14A G11,G12 52 Sodium Nitrite toxic anion

PM18C E02,E03,E04 48 Sodium metasilicate toxic anion

PM18C D07,D08 27 Sodium Bromate toxic anion

PM18C D02,D03,D04 22 Sodium Arsenite toxic anion

PM16A F08 21 Sodium Selenite toxic anion

PM17A E11 31 Sodium Tungstate toxic anion, molybdate analog

PM18C E07,E08 21 Sodium periodate toxic anion, oxidizing agent

PM14A H09,H10,H11,H12 90 Sodium orthovanadate toxic anion, PO4 analog

PM14A F09,F10,F11,F12 77 Sodium metavanadate toxic anion, PO4 analog

PM14A D09,D10,D11,D12 83 Sodium Dichromate toxic anion, SO4 analog

PM13C F09,F10,F11,F12 90 Thallium (I) acetate toxic cation

PM13C G02,G03,G04 85 Cobalt (II) chloride toxic cation

PM14A D03,D04 73 Cadmium chloride toxic cation

PM17A F02,F03,F04 70 Lithium chloride toxic cation

PM13C A11,A12 62 Nickel chloride toxic cation

PM18C E10,E11 44 Antimony (III) chloride toxic cation

PM13C H03 35 Cupric chloride toxic cation

PM09 G06,G07 38 200mM Sodium Benzoate pH 5.2 toxicity, benzoate

PM09 H10,H11 67 100mM Sodium Nitrite toxicity, nitrite

PM12B C10,C11,C12 105 DL-Serine hydroxamate tRNA synthetase

PM17A F06,F07,F08 104 DL-Methionine hydroxamate tRNA synthetase

PM16A G11,G12 73 L-Glutamic acid g-monohydroxamate tRNA synthetase

PM16A H02,H03,H04 19 Glycine hydroxamate tRNA synthetase

PM15B A04 15 Procaine wall, cephalosporin

PM12B B01,B02,B03,B04 60 Oxacillin wall, lactam

PM11C B06,B07,B08 40 Cloxacillin wall, lactam

PM13C B04 20 Azlocillin wall, lactam

PM14A G08 14 Carbenicillin wall, lactam

PM19 F04 7 Phenethicilllin wall, lactam

Phenotypes Lost:

Appendix:

Carbon and Nutrient Max Read Hour: 096

Nitrogen, Phosphorus, Sulfur Max Read Hour: 096

Sensitivity Max Read Hour: 048

Metabolic Distance Threshold: 060

Sensitivity Distance Threshold: 045

Test Data Files:

Replicate 1:

PM01 : J:\Service\Customer\300\366\Data\Assays\8962\bspp_SIDx6186xA396___1_25_962_110_Sepx29x2011_A_01A.oka

Replicate 2:

PM01 : J:\Service\Customer\300\366\Data\Assays\8975\bspp_SIDx6186xA396___1_25_975_110_Octx05x2011_A_01A.oka

Replicate 1:

PM02A: J:\Service\Customer\300\366\Data\Assays\8975\bspp_SIDx6186xA396___2_25_975_110_Octx05x2011_A_01B.oka

Replicate 2:

PM02A: J:\Service\Customer\300\366\Data\Assays\8962\bspp_SIDx6186xA396___2_25_962_110_Sepx29x2011_A_01B.oka

Replicate 1:

PM03B: J:\Service\Customer\300\366\Data\Assays\8962\bspp_SIDx6186xA396___3_25_962_110_Sepx29x2011_A_02A.oka

Replicate 2:

PM03B: J:\Service\Customer\300\366\Data\Assays\8975\bspp_SIDx6186xA396___3_25_975_110_Octx05x2011_A_02A.oka

Replicate 1:

PM04A: J:\Service\Customer\300\366\Data\Assays\8962\bspp_SIDx6186xA396___4_25_962_110_Sepx29x2011_A_02B.oka

Replicate 2:

PM04A: J:\Service\Customer\300\366\Data\Assays\8975\bspp_SIDx6186xA396___4_25_975_110_Octx05x2011_A_02B.oka

Replicate 1:

PM05 : J:\Service\Customer\300\366\Data\Assays\8975\bspp_SIDx6186xA396___5_25_975_110_Octx05x2011_A_03A.oka

Replicate 2:

PM05 : J:\Service\Customer\300\366\Data\Assays\8962\bspp_SIDx6186xA396___5_25_962_110_Sepx29x2011_A_03A.oka

Replicate 1:

PM06 : J:\Service\Customer\300\366\Data\Assays\8962\bspp_SIDx6186xA396___6_25_962_110_Sepx29x2011_A_03B.oka

Replicate 2:

PM06 : J:\Service\Customer\300\366\Data\Assays\8975\bspp_SIDx6186xA396___6_25_975_110_Octx05x2011_A_03B.oka

Replicate 1:

PM07 : J:\Service\Customer\300\366\Data\Assays\8962\bspp_SIDx6186xA396___7_25_962_110_Sepx29x2011_A_04A.oka

Replicate 2:

PM07 : J:\Service\Customer\300\366\Data\Assays\8975\bspp_SIDx6186xA396___7_25_975_110_Octx05x2011_A_04A.oka

Replicate 1:

PM08 : J:\Service\Customer\300\366\Data\Assays\8962\bspp_SIDx6186xA396___8_25_962_110_Sepx29x2011_A_04B.oka

Replicate 2:

PM08 : J:\Service\Customer\300\366\Data\Assays\8975\bspp_SIDx6186xA396___8_25_975_110_Octx05x2011_A_04B.oka

Replicate 1:

PM09 : J:\Service\Customer\300\366\Data\Assays\8962\bspp_SIDx6186xA396___9_25_962_110_Sepx29x2011_A_05A.oka

Replicate 2:

PM09 : J:\Service\Customer\300\366\Data\Assays\8975\bspp_SIDx6186xA396___9_25_975_110_Octx05x2011_A_05A.oka

Replicate 1:

PM10 : J:\Service\Customer\300\366\Data\Assays\8975\bspp_SIDx6186xA396___10_25_975_110_Octx05x2011_A_05B.oka

Replicate 2:

PM10 : J:\Service\Customer\300\366\Data\Assays\8962\bspp_SIDx6186xA396___10_25_962_110_Sepx29x2011_A_05B.oka

Replicate 1:

PM11C: J:\Service\Customer\300\366\Data\Assays\8962\bspp_SIDx6186xA396___11_25_962_110_Sepx29x2011_A_06A.oka

Replicate 2:

PM11C: J:\Service\Customer\300\366\Data\Assays\8975\bspp_SIDx6186xA396___11_25_975_110_Octx05x2011_A_06A.oka

Replicate 1:

PM12B: J:\Service\Customer\300\366\Data\Assays\8962\bspp_SIDx6186xA396___12_25_962_110_Sepx29x2011_A_06B.oka

Replicate 2:

PM12B: J:\Service\Customer\300\366\Data\Assays\8975\bspp_SIDx6186xA396___12_25_975_110_Octx05x2011_A_06B.oka

Replicate 1:

PM13C: J:\Service\Customer\300\366\Data\Assays\8962\bspp_SIDx6186xA396___13_25_962_110_Sepx29x2011_A_07A.oka

Replicate 2:

PM13C: J:\Service\Customer\300\366\Data\Assays\8975\bspp_SIDx6186xA396___13_25_975_110_Octx05x2011_A_07A.oka

Replicate 1:

PM14A: J:\Service\Customer\300\366\Data\Assays\8962\bspp_SIDx6186xA396___14_25_962_110_Sepx29x2011_A_07B.oka

Replicate 2:

PM14A: J:\Service\Customer\300\366\Data\Assays\8975\bspp_SIDx6186xA396___14_25_975_110_Octx05x2011_A_07B.oka

Replicate 1:

PM15B: J:\Service\Customer\300\366\Data\Assays\8962\bspp_SIDx6186xA396___15_25_962_110_Sepx29x2011_A_08A.oka

Replicate 2:

PM15B: J:\Service\Customer\300\366\Data\Assays\8975\bspp_SIDx6186xA396___15_25_975_110_Octx05x2011_A_08A.oka

Replicate 1:

PM16A: J:\Service\Customer\300\366\Data\Assays\8962\bspp_SIDx6186xA396___16_25_962_110_Sepx29x2011_A_08B.oka

Replicate 2:

PM16A: J:\Service\Customer\300\366\Data\Assays\8975\bspp_SIDx6186xA396___16_25_975_110_Octx05x2011_A_08B.oka

Replicate 1:

PM17A: J:\Service\Customer\300\366\Data\Assays\8962\bspp_SIDx6186xA396___17_25_962_110_Sepx29x2011_A_09A.oka

Replicate 2:

PM17A: J:\Service\Customer\300\366\Data\Assays\8975\bspp_SIDx6186xA396___17_25_975_110_Octx05x2011_A_09A.oka

Replicate 1:

PM18C: J:\Service\Customer\300\366\Data\Assays\8962\bspp_SIDx6186xA396___18_25_962_110_Sepx29x2011_A_09B.oka

Replicate 2:

PM18C: J:\Service\Customer\300\366\Data\Assays\8975\bspp_SIDx6186xA396___18_25_975_110_Octx05x2011_A_09B.oka

Replicate 1:

PM19 : J:\Service\Customer\300\366\Data\Assays\8975\bspp_SIDx6186xA396___19_25_975_110_Octx05x2011_A_10A.oka

Replicate 2:

PM19 : J:\Service\Customer\300\366\Data\Assays\8962\bspp_SIDx6186xA396___19_25_962_110_Sepx29x2011_A_10A.oka

Replicate 1:

PM20B: J:\Service\Customer\300\366\Data\Assays\8962\bspp_SIDx6186xA396___20_25_962_110_Sepx29x2011_A_10B.oka

Replicate 2:

PM20B: J:\Service\Customer\300\366\Data\Assays\8975\bspp_SIDx6186xA396___20_25_975_110_Octx05x2011_A_10B.oka

Reference Data Files: