Supplemental figures.
11
Supplemental figures. Observed extracted ion chromatograms (XICs) of each of the ten peptides from five standard proteins using dual ion funnel and standard Thermo interface. Data were analyzed using Thermo Xcalibur 2.0.7 with Qual Browser. Six standard peptides, bradykinin fragment 1-7, kemptide, melittin, methionine enkephalin, renin substrate porcine, and [D-Ala2]-deltorphin II were used as QC peptides for LC retention time markers and for monitoring the overall performance of the platform. The peak widths for each peptide were determined by averaging the data obtained using two highest spiked concentrations (S8 and S9) and the elution time difference observed relative to the nearest QC marker peaks served as a peak integration window for the lower (S0-S7) spike concentrations. Analyte peak identification at the lower concentrations were determined based on three main criteria: 1) difference of elution time from nearest QC peaks within ±10% of the average difference observed from the two highest concentrations; 2) analyte peak widths were similar (i.e., within ±10%) to the average of peak widths from the two top concentrations; and 3) analyte peak area decreases with decreasing analyte concentration levels. On each of the following page, the top and bottom panels represent XICs from dual ion funnel interface and standard Thermo interface experiments, respectively. Top three spectra on each panel are three selective transitions from a specific peptide. One of the best transitions is selected for LOD and reproducibility analysis for each peptide. All of the transitions used in this study are listed in supplemental Table 1. The bottom spectrum is of the nearest QC peptide used as an elution marker for peak identification. For comparative study, representative spectra from LOD concentration, one concentration above and below this concentration, and highest concentration are presented using both dual ion funnel and standard Thermo interface. Analyte peaks of interest identified are shaded in gray for ease of visualization and arrows on the extreme right hand side spectra (lower concentration) indicate possible analyte peak position embedded in / interfered with the noise levels / matrix peaks limiting the detection.
description
Supplemental figures. - PowerPoint PPT Presentation
Transcript of Supplemental figures.
Supplemental figures.
Observed extracted ion chromatograms (XICs) of each of the ten peptides from five standard proteins using dual ion funnel and standard Thermo interface. Data were analyzed using Thermo Xcalibur 2.0.7 with Qual Browser. Six standard peptides, bradykinin fragment 1-7, kemptide, melittin, methionine enkephalin, renin substrate porcine, and [D-Ala2]-deltorphin II were used as QC peptides for LC retention time markers and for monitoring the overall performance of the platform. The peak widths for each peptide were determined by averaging the data obtained using two highest spiked concentrations (S8 and S9) and the elution time difference observed relative to the nearest QC marker peaks served as a peak integration window for the lower (S0-S7) spike concentrations. Analyte peak identification at the lower concentrations were determined based on three main criteria: 1) difference of elution time from nearest QC peaks within ±10% of the average difference observed from the two highest concentrations; 2) analyte peak widths were similar (i.e., within ±10%) to the average of peak widths from the two top concentrations; and 3) analyte peak area decreases with decreasing analyte concentration levels.
On each of the following page, the top and bottom panels represent XICs from dual ion funnel interface and standard Thermo interface experiments, respectively. Top three spectra on each panel are three selective transitions from a specific peptide. One of the best transitions is selected for LOD and reproducibility analysis for each peptide. All of the transitions used in this study are listed in supplemental Table 1. The bottom spectrum is of the nearest QC peptide used as an elution marker for peak identification. For comparative study, representative spectra from LOD concentration, one concentration above and below this concentration, and highest concentration are presented using both dual ion funnel and standard Thermo interface. Analyte peaks of interest identified are shaded in gray for ease of visualization and arrows on the extreme right hand side spectra (lower concentration) indicate possible analyte peak position embedded in / interfered with the noise levels / matrix peaks limiting the detection.
40 µg/mL 400 ng/mL 80 ng/mL (LOD) 40 ng/mL
40 µg/mL 4 µg/mL 400 ng/mL (LOD) 80 ng/mL
a) Dual ion funnel interface
b) Standard Thermo interface
A: XICs of peptide VLDALDSIK. LODs were determined for transition of m/z 487.282+ > 874.49+ . Methionine enkephalin (transition m/z 574.162+ > 397.24+) was used here as a retention time marker.
RT: 30.00 - 35.00 SM: 15G
30.0 30.5 31.0 31.5 32.0 32.5 33.0 33.5 34.0 34.5 35.00
50000000
100000000
150000000
200000000
250000000
0
5000000
10000000
15000000
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20000000
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60000000
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NL: 1.78E7Base Peak m/z= 645.88-646.88 F: + c NSI SRM ms2 487.282 [646.376-646.378, 761.403-761.405, 874.487-874.489] MS IFIE_MP_0p5_S9_22dec08_2F_Kestrel_08-11-06_S9_2a
NL: 1.19E8Base Peak m/z= 760.90-761.90 F: + c NSI SRM ms2 487.282 [646.376-646.378, 761.403-761.405, 874.487-874.489] MS IFIE_MP_0p5_S9_22dec08_2F_Kestrel_08-11-06_S9_2a
NL: 1.92E7Base Peak m/z= 873.99-874.99 F: + c NSI SRM ms2 487.282 [646.376-646.378, 761.403-761.405, 874.487-874.489] MS IFIE_MP_0p5_S9_22dec08_2F_Kestrel_08-11-06_S9_2a
NL: 2.91E8Base Peak m/z= 396.75-397.75 F: + c NSI SRM ms2 574.162 [397.248-397.250, 425.209-425.211] MS IFIE_MP_0p5_S9_22dec08_2F_Kestrel_08-11-06_S9_2a
RT: 32.50 - 37.50 SM: 15G
32.5 33.0 33.5 34.0 34.5 35.0 35.5 36.0 36.5 37.0 37.50
50000000
100000000
150000000
200000000
250000000
0
50000
100000
150000
2000000
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600000
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0
100000
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400000NL: 4.22E5Base Peak m/z= 645.88-646.88 F: + c NSI SRM ms2 487.282 [646.376-646.378, 761.403-761.405, 874.487-874.489] MS IFIE_MP_0p5_S7_22dec08_2F_Kestrel_08-11-06_S7_2b
NL: 8.80E5Base Peak m/z= 760.90-761.90 F: + c NSI SRM ms2 487.282 [646.376-646.378, 761.403-761.405, 874.487-874.489] MS IFIE_MP_0p5_S7_22dec08_2F_Kestrel_08-11-06_S7_2b
NL: 2.11E5Base Peak m/z= 873.99-874.99 F: + c NSI SRM ms2 487.282 [646.376-646.378, 761.403-761.405, 874.487-874.489] MS IFIE_MP_0p5_S7_22dec08_2F_Kestrel_08-11-06_S7_2b
NL: 2.91E8Base Peak m/z= 396.75-397.75 F: + c NSI SRM ms2 574.162 [397.248-397.250, 425.209-425.211] MS IFIE_MP_0p5_S7_22dec08_2F_Kestrel_08-11-06_S7_2b
RT: 32.50 - 37.50 SM: 15G
32.5 33.0 33.5 34.0 34.5 35.0 35.5 36.0 36.5 37.0 37.50
50000000
100000000
150000000
200000000
250000000
0
50000
100000
150000
0
100000
200000
300000
400000
500000
600000
0
100000
200000
300000
400000
500000 NL: 5.11E5Base Peak m/z= 645.88-646.88 F: + c NSI SRM ms2 487.282 [646.376-646.378, 761.403-761.405, 874.487-874.489] MS IFIE_MP_0p5_S6_22dec08_2F_Kestrel_08-11-06_S6_2a
NL: 6.57E5Base Peak m/z= 760.90-761.90 F: + c NSI SRM ms2 487.282 [646.376-646.378, 761.403-761.405, 874.487-874.489] MS IFIE_MP_0p5_S6_22dec08_2F_Kestrel_08-11-06_S6_2a
NL: 1.86E5Base Peak m/z= 873.99-874.99 F: + c NSI SRM ms2 487.282 [646.376-646.378, 761.403-761.405, 874.487-874.489] MS IFIE_MP_0p5_S6_22dec08_2F_Kestrel_08-11-06_S6_2a
NL: 2.91E8Base Peak m/z= 396.75-397.75 F: + c NSI SRM ms2 574.162 [397.248-397.250, 425.209-425.211] MS IFIE_MP_0p5_S6_22dec08_2F_Kestrel_08-11-06_S6_2a
RT: 30.50 - 35.50 SM: 15G
30.5 31.0 31.5 32.0 32.5 33.0 33.5 34.0 34.5 35.0 35.50
50000000
100000000
150000000
200000000
250000000
0
20000
40000
60000
80000
100000
0
50000
100000
150000
200000
250000
300000
350000
4000000
50000
100000
150000
200000
250000
300000
350000
400000 NL: 4.03E5Base Peak m/z= 645.88-646.88 F: + c NSI SRM ms2 487.282 [646.376-646.378, 761.403-761.405, 874.487-874.489] MS IFIE_MP_0p5_S5_22dec08_2F_Kestrel_08-11-06_S5_2a
NL: 4.14E5Base Peak m/z= 760.90-761.90 F: + c NSI SRM ms2 487.282 [646.376-646.378, 761.403-761.405, 874.487-874.489] MS IFIE_MP_0p5_S5_22dec08_2F_Kestrel_08-11-06_S5_2a
NL: 1.16E5Base Peak m/z= 873.99-874.99 F: + c NSI SRM ms2 487.282 [646.376-646.378, 761.403-761.405, 874.487-874.489] MS IFIE_MP_0p5_S5_22dec08_2F_Kestrel_08-11-06_S5_2a
NL: 2.91E8Base Peak m/z= 396.75-397.75 F: + c NSI SRM ms2 574.162 [397.248-397.250, 425.209-425.211] MS IFIE_MP_0p5_S5_22dec08_2F_Kestrel_08-11-06_S5_2a
RT: 29.50 - 34.50 SM: 15G
29.5 30.0 30.5 31.0 31.5 32.0 32.5 33.0 33.5 34.0 34.50
5000000
10000000
15000000
0
100000
200000
300000
400000
0
500000
1000000
1500000
2000000
2500000
3000000
3500000
0
100000
200000
300000
400000NL: 4.36E5Base Peak m/z= 645.88-646.88 F: + c NSI SRM ms2 487.282 [646.376-646.378, 761.403-761.405, 874.487-874.489] MS IFIE_MP_0p5_S9_04sep08_Th_Kestrel_08-07-16_S9_2a
NL: 3.96E6Base Peak m/z= 760.90-761.90 F: + c NSI SRM ms2 487.282 [646.376-646.378, 761.403-761.405, 874.487-874.489] MS IFIE_MP_0p5_S9_04sep08_Th_Kestrel_08-07-16_S9_2a
NL: 4.99E5Base Peak m/z= 873.99-874.99 F: + c NSI SRM ms2 487.282 [646.376-646.378, 761.403-761.405, 874.487-874.489] MS Genesis IFIE_MP_0p5_S9_04sep08_Th_Kestrel_08-07-16_S9_2a
NL: 1.99E7Base Peak m/z= 396.75-397.75 F: + c NSI SRM ms2 574.162 [397.248-397.250, 425.209-425.211] MS IFIE_MP_0p5_S9_04sep08_Th_Kestrel_08-07-16_S9_2a
RT: 28.50 - 33.50 SM: 15G
28.5 29.0 29.5 30.0 30.5 31.0 31.5 32.0 32.5 33.0 33.50
5000000
10000000
15000000
200000000
10000
20000
30000
40000
50000
0
100000
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400000
0
10000
20000
30000
40000
NL: 4.52E4Base Peak m/z= 645.88-646.88 F: + c NSI SRM ms2 487.282 [646.376-646.378, 761.403-761.405, 874.487-874.489] MS IFIE_MP_0p5_S8_04sep08_Th_Kestrel_08-07-16_S8_2c
NL: 4.55E5Base Peak m/z= 760.90-761.90 F: + c NSI SRM ms2 487.282 [646.376-646.378, 761.403-761.405, 874.487-874.489] MS IFIE_MP_0p5_S8_04sep08_Th_Kestrel_08-07-16_S8_2c
NL: 5.87E4Base Peak m/z= 873.99-874.99 F: + c NSI SRM ms2 487.282 [646.376-646.378, 761.403-761.405, 874.487-874.489] MS IFIE_MP_0p5_S8_04sep08_Th_Kestrel_08-07-16_S8_2c
NL: 2.14E7Base Peak m/z= 396.75-397.75 F: + c NSI SRM ms2 574.162 [397.248-397.250, 425.209-425.211] MS IFIE_MP_0p5_S8_04sep08_Th_Kestrel_08-07-16_S8_2c
RT: 28.50 - 33.50 SM: 15G
28.5 29.0 29.5 30.0 30.5 31.0 31.5 32.0 32.5 33.0 33.50
5000000
10000000
15000000
20000000
0
1000
2000
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5000
6000
7000
0
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15000
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30000
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6000NL: 6.63E3Base Peak m/z= 645.88-646.88 F: + c NSI SRM ms2 487.282 [646.376-646.378, 761.403-761.405, 874.487-874.489] MS IFIE_MP_0p5_S7_04sep08_Th_Kestrel_08-07-16_S7_2c
NL: 4.17E4Base Peak m/z= 760.90-761.90 F: + c NSI SRM ms2 487.282 [646.376-646.378, 761.403-761.405, 874.487-874.489] MS IFIE_MP_0p5_S7_04sep08_Th_Kestrel_08-07-16_S7_2c
NL: 7.68E3Base Peak m/z= 873.99-874.99 F: + c NSI SRM ms2 487.282 [646.376-646.378, 761.403-761.405, 874.487-874.489] MS IFIE_MP_0p5_S7_04sep08_Th_Kestrel_08-07-16_S7_2c
NL: 2.34E7Base Peak m/z= 396.75-397.75 F: + c NSI SRM ms2 574.162 [397.248-397.250, 425.209-425.211] MS IFIE_MP_0p5_S7_04sep08_Th_Kestrel_08-07-16_S7_2c
RT: 28.00 - 33.00 SM: 15G
28.0 28.5 29.0 29.5 30.0 30.5 31.0 31.5 32.0 32.5 33.00
5000000
10000000
15000000
0
2000
4000
6000
8000
0
5000
10000
15000
200000
1000
2000
3000
4000
5000
6000 NL: 6.15E3Base Peak m/z= 645.88-646.88 F: + c NSI SRM ms2 487.282 [646.376-646.378, 761.403-761.405, 874.487-874.489] MS IFIE_MP_0p5_S6_04sep08_Th_Kestrel_08-07-16_S6_2c
NL: 2.10E4Base Peak m/z= 760.90-761.90 F: + c NSI SRM ms2 487.282 [646.376-646.378, 761.403-761.405, 874.487-874.489] MS IFIE_MP_0p5_S6_04sep08_Th_Kestrel_08-07-16_S6_2c
NL: 8.53E3Base Peak m/z= 873.99-874.99 F: + c NSI SRM ms2 487.282 [646.376-646.378, 761.403-761.405, 874.487-874.489] MS IFIE_MP_0p5_S6_04sep08_Th_Kestrel_08-07-16_S6_2c
NL: 1.91E7Base Peak m/z= 396.75-397.75 F: + c NSI SRM ms2 574.162 [397.248-397.250, 425.209-425.211] MS IFIE_MP_0p5_S6_04sep08_Th_Kestrel_08-07-16_S6_2c
40 µg/mL 80 ng/mL 40 ng/mL (LOD) 8 ng/mL
40 µg/mL 400 ng/mL 80 ng/mL (LOD) 40 ng/mL
a) Dual ion funnel interface
b) Standard Thermo interface
B: XICs of peptide DFPIANGER. LODs were determined for transition of m/z 509.752+ > 378.70+ . Bradykinin fragment 1-7 (transition m/z 379.142+ > 527.31+) was used here as a retention time marker.
RT: 23.50 - 28.50 SM: 15G
23.5 24.0 24.5 25.0 25.5 26.0 26.5 27.0 27.5 28.0 28.50
20000000
40000000
60000000
80000000
100000000
120000000
140000000
1600000000
2000000
4000000
6000000
8000000
10000000
120000000
2000000
4000000
6000000
8000000
10000000
12000000
0
5000000
10000000
15000000
20000000
25000000
NL: 2.98E7Base Peak m/z= 378.20-379.20 F: + c NSI SRM ms2 509.751 [378.703-378.705, 546.262-546.264, 756.399-756.401] MS IFIE_MP_0p5_S9_22dec08_2F_Kestrel_08-11-06_S9_2c
NL: 1.29E7Base Peak m/z= 545.76-546.76 F: + c NSI SRM ms2 509.751 [378.703-378.705, 546.262-546.264, 756.399-756.401] MS IFIE_MP_0p5_S9_22dec08_2F_Kestrel_08-11-06_S9_2c
NL: 1.25E7Base Peak m/z= 755.90-756.90 F: + c NSI SRM ms2 509.751 [378.703-378.705, 546.262-546.264, 756.399-756.401] MS IFIE_MP_0p5_S9_22dec08_2F_Kestrel_08-11-06_S9_2c
NL: 1.64E8Base Peak F: + c NSI SRM ms2 379.142 [527.311-527.313, 614.355-614.357] MS IFIE_MP_0p5_S9_22dec08_2F_Kestrel_08-11-06_S9_2c
RT: 22.00 - 27.10 SM: 15G
22.0 22.5 23.0 23.5 24.0 24.5 25.0 25.5 26.0 26.5 27.00
20000000
40000000
60000000
80000000
100000000
1200000000
20000
40000
60000
80000
100000
120000
140000
1600000
50000
100000
150000
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250000
0
50000
100000
150000
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250000 NL: 2.56E5Base Peak m/z= 378.20-379.20 F: + c NSI SRM ms2 509.751 [378.703-378.705, 546.262-546.264, 756.399-756.401] MS IFIE_MP_0p5_S5_22dec08_2F_Kestrel_08-11-06_S5_2c
NL: 2.84E5Base Peak m/z= 545.76-546.76 F: + c NSI SRM ms2 509.751 [378.703-378.705, 546.262-546.264, 756.399-756.401] MS IFIE_MP_0p5_S5_22dec08_2F_Kestrel_08-11-06_S5_2c
NL: 1.62E5Base Peak m/z= 755.90-756.90 F: + c NSI SRM ms2 509.751 [378.703-378.705, 546.262-546.264, 756.399-756.401] MS IFIE_MP_0p5_S5_22dec08_2F_Kestrel_08-11-06_S5_2c
NL: 1.27E8Base Peak F: + c NSI SRM ms2 379.142 [527.311-527.313, 614.355-614.357] MS IFIE_MP_0p5_S5_22dec08_2F_Kestrel_08-11-06_S5_2c
RT: 23.50 - 28.15 SM: 15G
23.5 24.0 24.5 25.0 25.5 26.0 26.5 27.0 27.5 28.00
20000000
40000000
60000000
80000000
100000000
120000000
140000000
0
20000
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100000
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0
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NL: 1.80E5Base Peak m/z= 378.20-379.20 F: + c NSI SRM ms2 509.751 [378.703-378.705, 546.262-546.264, 756.399-756.401] MS IFIE_MP_0p5_S4_22dec08_2F_Kestrel_08-11-06_S4_2c
NL: 2.86E5Base Peak m/z= 545.76-546.76 F: + c NSI SRM ms2 509.751 [378.703-378.705, 546.262-546.264, 756.399-756.401] MS IFIE_MP_0p5_S4_22dec08_2F_Kestrel_08-11-06_S4_2c
NL: 1.51E5Base Peak m/z= 755.90-756.90 F: + c NSI SRM ms2 509.751 [378.703-378.705, 546.262-546.264, 756.399-756.401] MS IFIE_MP_0p5_S4_22dec08_2F_Kestrel_08-11-06_S4_2c
NL: 1.58E8Base Peak F: + c NSI SRM ms2 379.142 [527.311-527.313, 614.355-614.357] MS IFIE_MP_0p5_S4_22dec08_2F_Kestrel_08-11-06_S4_2c
RT: 21.75 - 26.25 SM: 15G
22.0 22.5 23.0 23.5 24.0 24.5 25.0 25.5 26.00
20000000
40000000
60000000
80000000
100000000
120000000
140000000
0
20000
40000
60000
80000
100000
120000
140000
0
50000
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0
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40000
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NL: 9.83E4Base Peak m/z= 378.20-379.20 F: + c NSI SRM ms2 509.751 [378.703-378.705, 546.262-546.264, 756.399-756.401] MS IFIE_MP_0p5_S3_22dec08_2F_Kestrel_08-11-06_S3_2b
NL: 2.85E5Base Peak m/z= 545.76-546.76 F: + c NSI SRM ms2 509.751 [378.703-378.705, 546.262-546.264, 756.399-756.401] MS IFIE_MP_0p5_S3_22dec08_2F_Kestrel_08-11-06_S3_2b
NL: 1.56E5Base Peak m/z= 755.90-756.90 F: + c NSI SRM ms2 509.751 [378.703-378.705, 546.262-546.264, 756.399-756.401] MS IFIE_MP_0p5_S3_22dec08_2F_Kestrel_08-11-06_S3_2b
NL: 1.52E8Base Peak F: + c NSI SRM ms2 379.142 [527.311-527.313, 614.355-614.357] MS IFIE_MP_0p5_S3_22dec08_2F_Kestrel_08-11-06_S3_2b
RT: 20.00 - 25.00 SM: 15G
20.0 20.5 21.0 21.5 22.0 22.5 23.0 23.5 24.0 24.5 25.00
1000000
2000000
3000000
4000000
5000000
60000000
50000
100000
150000
200000
250000
0
50000
100000
150000
0
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600000
800000
1000000
1200000NL: 1.29E6Base Peak m/z= 378.20-379.20 F: + c NSI SRM ms2 509.751 [378.703-378.705, 546.262-546.264, 756.399-756.401] MS IFIE_MP_0p5_S9_04sep08_Th_Kestrel_08-07-16_S9_2c
NL: 1.74E5Base Peak m/z= 545.76-546.76 F: + c NSI SRM ms2 509.751 [378.703-378.705, 546.262-546.264, 756.399-756.401] MS IFIE_MP_0p5_S9_04sep08_Th_Kestrel_08-07-16_S9_2c
NL: 2.74E5Base Peak m/z= 755.90-756.90 F: + c NSI SRM ms2 509.751 [378.703-378.705, 546.262-546.264, 756.399-756.401] MS IFIE_MP_0p5_S9_04sep08_Th_Kestrel_08-07-16_S9_2c
NL: 6.06E6Base Peak F: + c NSI SRM ms2 379.142 [527.311-527.313, 614.355-614.357] MS IFIE_MP_0p5_S9_04sep08_Th_Kestrel_08-07-16_S9_2c
RT: 20.50 - 25.00 SM: 15G
20.5 21.0 21.5 22.0 22.5 23.0 23.5 24.0 24.5 25.00
1000000
2000000
3000000
4000000
5000000
6000000
7000000
0
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16000 NL: 1.64E4Base Peak m/z= 378.20-379.20 F: + c NSI SRM ms2 509.751 [378.703-378.705, 546.262-546.264, 756.399-756.401] MS IFIE_MP_0p5_S7_04sep08_Th_Kestrel_08-07-16_S7_2c
NL: 1.69E4Base Peak m/z= 545.76-546.76 F: + c NSI SRM ms2 509.751 [378.703-378.705, 546.262-546.264, 756.399-756.401] MS IFIE_MP_0p5_S7_04sep08_Th_Kestrel_08-07-16_S7_2c
NL: 6.76E3Base Peak m/z= 755.90-756.90 F: + c NSI SRM ms2 509.751 [378.703-378.705, 546.262-546.264, 756.399-756.401] MS IFIE_MP_0p5_S7_04sep08_Th_Kestrel_08-07-16_S7_2c
NL: 7.55E6Base Peak F: + c NSI SRM ms2 379.142 [527.311-527.313, 614.355-614.357] MS IFIE_MP_0p5_S7_04sep08_Th_Kestrel_08-07-16_S7_2c
RT: 20.00 - 24.50 SM: 15G
20.0 20.5 21.0 21.5 22.0 22.5 23.0 23.5 24.0 24.50
1000000
2000000
3000000
4000000
5000000
6000000
70000000
1000
2000
3000
4000
5000
6000
70000
2000
4000
6000
8000
10000
0
200
400
600
800
1000
1200NL: 1.26E3Base Peak m/z= 378.20-379.20 F: + c NSI SRM ms2 509.751 [378.703-378.705, 546.262-546.264, 756.399-756.401] MS IFIE_MP_0p5_S5_04sep08_Th_Kestrel_08-07-16_S5_2b
NL: 1.13E4Base Peak m/z= 545.76-546.76 F: + c NSI SRM ms2 509.751 [378.703-378.705, 546.262-546.264, 756.399-756.401] MS IFIE_MP_0p5_S5_04sep08_Th_Kestrel_08-07-16_S5_2b
NL: 7.28E3Base Peak m/z= 755.90-756.90 F: + c NSI SRM ms2 509.751 [378.703-378.705, 546.262-546.264, 756.399-756.401] MS IFIE_MP_0p5_S5_04sep08_Th_Kestrel_08-07-16_S5_2b
NL: 7.13E6Base Peak F: + c NSI SRM ms2 379.142 [527.311-527.313, 614.355-614.357] MS IFIE_MP_0p5_S5_04sep08_Th_Kestrel_08-07-16_S5_2b
RT: 19.50 - 24.50 SM: 15G
19.5 20.0 20.5 21.0 21.5 22.0 22.5 23.0 23.5 24.0 24.50
2000000
4000000
6000000
8000000
0
500
1000
1500
2000
2500
3000
35000
2000
4000
6000
8000
10000
12000
0
500
1000
1500
2000
2500
3000
3500
4000 NL: 4.02E3Base Peak m/z= 378.20-379.20 F: + c NSI SRM ms2 509.751 [378.703-378.705, 546.262-546.264, 756.399-756.401] MS IFIE_MP_0p5_S6_04sep08_Th_Kestrel_08-07-16_S6_2c
NL: 1.33E4Base Peak m/z= 545.76-546.76 F: + c NSI SRM ms2 509.751 [378.703-378.705, 546.262-546.264, 756.399-756.401] MS IFIE_MP_0p5_S6_04sep08_Th_Kestrel_08-07-16_S6_2c
NL: 3.72E3Base Peak m/z= 755.90-756.90 F: + c NSI SRM ms2 509.751 [378.703-378.705, 546.262-546.264, 756.399-756.401] MS IFIE_MP_0p5_S6_04sep08_Th_Kestrel_08-07-16_S6_2c
NL: 9.15E6Base Peak F: + c NSI SRM ms2 379.142 [527.311-527.313, 614.355-614.357] MS IFIE_MP_0p5_S6_04sep08_Th_Kestrel_08-07-16_S6_2c
40 µg/mL 400 ng/mL 80 ng/mL (LOD) 40 ng/mL
40 µg/mL 4 µg/mL 400 ng/mL (LOD) 80 ng/mL
a) Dual ion funnel interface
b) Standard Thermo interface
C: XICs of peptide VDEDQPFPAVPK. LODs were determined for transition of m/z 671.342+ > 755.45+ . Methionine enkephalin(transition m/z 574.162+ > 397.25+) was used here as a retention time marker .
RT: 28.89 - 33.34 SM: 15G
29.0 29.5 30.0 30.5 31.0 31.5 32.0 32.5 33.00
50000000
100000000
150000000
200000000
250000000
0
2000000
4000000
6000000
8000000
10000000
12000000
0
1000000
2000000
3000000
4000000
50000000
2000000
4000000
6000000
8000000
10000000
12000000
14000000
16000000 NL: 1.60E7Base Peak m/z= 243.67-244.67 F: + c NSI SRM ms2 671.338 [244.165-244.167, 511.323-511.325, 755.454-755.456] MS IFIE_MP_0p5_S9_22dec08_2F_Kestrel_08-11-06_S9_2a
NL: 5.30E6Base Peak m/z= 510.82-511.82 F: + c NSI SRM ms2 671.338 [244.165-244.167, 511.323-511.325, 755.454-755.456] MS IFIE_MP_0p5_S9_22dec08_2F_Kestrel_08-11-06_S9_2a
NL: 1.37E7Base Peak m/z= 754.95-755.95 F: + c NSI SRM ms2 671.338 [244.165-244.167, 511.323-511.325, 755.454-755.456] MS IFIE_MP_0p5_S9_22dec08_2F_Kestrel_08-11-06_S9_2a
NL: 2.91E8Base Peak F: + c NSI SRM ms2 574.162 [397.248-397.250, 425.209-425.211] MS IFIE_MP_0p5_S9_22dec08_2F_Kestrel_08-11-06_S9_2a
RT: 31.50 - 35.50 SM: 15G
31.5 32.0 32.5 33.0 33.5 34.0 34.5 35.0 35.50
50000000
100000000
150000000
200000000
250000000
0
50000
100000
150000
0
500000
1000000
1500000
0
50000
100000
150000
200000
250000
300000
350000
400000 NL: 4.13E5Base Peak m/z= 243.67-244.67 F: + c NSI SRM ms2 671.338 [244.165-244.167, 511.323-511.325, 755.454-755.456] MS IFIE_MP_0p5_S6_22dec08_2F_Kestrel_08-11-06_S6_2a
NL: 1.99E6Base Peak m/z= 510.82-511.82 F: + c NSI SRM ms2 671.338 [244.165-244.167, 511.323-511.325, 755.454-755.456] MS IFIE_MP_0p5_S6_22dec08_2F_Kestrel_08-11-06_S6_2a
NL: 2.00E5Base Peak m/z= 754.95-755.95 F: + c NSI SRM ms2 671.338 [244.165-244.167, 511.323-511.325, 755.454-755.456] MS IFIE_MP_0p5_S6_22dec08_2F_Kestrel_08-11-06_S6_2a
NL: 2.91E8Base Peak F: + c NSI SRM ms2 574.162 [397.248-397.250, 425.209-425.211] MS IFIE_MP_0p5_S6_22dec08_2F_Kestrel_08-11-06_S6_2a
RT: 29.50 - 33.50 SM: 15G
29.5 30.0 30.5 31.0 31.5 32.0 32.5 33.0 33.50
50000000
100000000
150000000
200000000
250000000
0
20000
40000
60000
80000
100000
120000
140000
0
200000
400000
600000
800000
1000000
1200000
1400000
0
200000
400000
600000
800000
NL: 9.19E5Base Peak m/z= 243.67-244.67 F: + c NSI SRM ms2 671.338 [244.165-244.167, 511.323-511.325, 755.454-755.456] MS IFIE_MP_0p5_S5_22dec08_2F_Kestrel_08-11-06_S5_2a
NL: 1.52E6Base Peak m/z= 510.82-511.82 F: + c NSI SRM ms2 671.338 [244.165-244.167, 511.323-511.325, 755.454-755.456] MS IFIE_MP_0p5_S5_22dec08_2F_Kestrel_08-11-06_S5_2a
NL: 1.52E5Base Peak m/z= 754.95-755.95 F: + c NSI SRM ms2 671.338 [244.165-244.167, 511.323-511.325, 755.454-755.456] MS IFIE_MP_0p5_S5_22dec08_2F_Kestrel_08-11-06_S5_2a
NL: 2.91E8Base Peak F: + c NSI SRM ms2 574.162 [397.248-397.250, 425.209-425.211] MS IFIE_MP_0p5_S5_22dec08_2F_Kestrel_08-11-06_S5_2a
RT: 30.50 - 34.50 SM: 15G
30.5 31.0 31.5 32.0 32.5 33.0 33.5 34.0 34.50
50000000
100000000
150000000
200000000
250000000
0
20000
40000
60000
80000
100000
120000
140000
0
500000
1000000
1500000
0
100000
200000
300000
400000
NL: 4.51E5Base Peak m/z= 243.67-244.67 F: + c NSI SRM ms2 671.338 [244.165-244.167, 511.323-511.325, 755.454-755.456] MS IFIE_MP_0p5_S4_22dec08_2F_Kestrel_08-11-06_S4_2a
NL: 1.72E6Base Peak m/z= 510.82-511.82 F: + c NSI SRM ms2 671.338 [244.165-244.167, 511.323-511.325, 755.454-755.456] MS IFIE_MP_0p5_S4_22dec08_2F_Kestrel_08-11-06_S4_2a
NL: 1.52E5Base Peak m/z= 754.95-755.95 F: + c NSI SRM ms2 671.338 [244.165-244.167, 511.323-511.325, 755.454-755.456] MS IFIE_MP_0p5_S4_22dec08_2F_Kestrel_08-11-06_S4_2a
NL: 2.91E8Base Peak F: + c NSI SRM ms2 574.162 [397.248-397.250, 425.209-425.211] MS IFIE_MP_0p5_S4_22dec08_2F_Kestrel_08-11-06_S4_2a
RT: 26.00 - 31.50 SM: 15G
26.0 26.5 27.0 27.5 28.0 28.5 29.0 29.5 30.0 30.5 31.0 31.50
2000000
4000000
6000000
8000000
10000000
0
50000
100000
150000
0
10000
20000
30000
40000
50000
600000
50000
100000
150000
200000NL: 2.15E5Base Peak m/z= 243.67-244.67 F: + c NSI SRM ms2 671.338 [244.165-244.167, 511.323-511.325, 755.454-755.456] MS IFIE_MP_0p5_S9_04sep08_Th_Kestrel_08-07-16_S9_2c
NL: 6.33E4Base Peak m/z= 510.82-511.82 F: + c NSI SRM ms2 671.338 [244.165-244.167, 511.323-511.325, 755.454-755.456] MS IFIE_MP_0p5_S9_04sep08_Th_Kestrel_08-07-16_S9_2c
NL: 1.76E5Base Peak m/z= 754.95-755.95 F: + c NSI SRM ms2 671.338 [244.165-244.167, 511.323-511.325, 755.454-755.456] MS IFIE_MP_0p5_S9_04sep08_Th_Kestrel_08-07-16_S9_2c
NL: 1.19E7Base Peak F: + c NSI SRM ms2 574.162 [397.248-397.250, 425.209-425.211] MS IFIE_MP_0p5_S9_04sep08_Th_Kestrel_08-07-16_S9_2c
RT: 26.50 - 32.00 SM: 15G
26.5 27.0 27.5 28.0 28.5 29.0 29.5 30.0 30.5 31.0 31.5 32.00
5000000
10000000
15000000
200000000
5000
10000
15000
0
10000
20000
30000
40000
0
5000
10000
15000
20000
25000
NL: 2.93E4Base Peak m/z= 243.67-244.67 F: + c NSI SRM ms2 671.338 [244.165-244.167, 511.323-511.325, 755.454-755.456] MS IFIE_MP_0p5_S8_04sep08_Th_Kestrel_08-07-16_S8_2c
NL: 4.95E4Base Peak m/z= 510.82-511.82 F: + c NSI SRM ms2 671.338 [244.165-244.167, 511.323-511.325, 755.454-755.456] MS IFIE_MP_0p5_S8_04sep08_Th_Kestrel_08-07-16_S8_2c
NL: 1.99E4Base Peak m/z= 754.95-755.95 F: + c NSI SRM ms2 671.338 [244.165-244.167, 511.323-511.325, 755.454-755.456] MS IFIE_MP_0p5_S8_04sep08_Th_Kestrel_08-07-16_S8_2c
NL: 2.14E7Base Peak F: + c NSI SRM ms2 574.162 [397.248-397.250, 425.209-425.211] MS IFIE_MP_0p5_S8_04sep08_Th_Kestrel_08-07-16_S8_2c
RT: 26.50 - 32.00 SM: 15G
26.5 27.0 27.5 28.0 28.5 29.0 29.5 30.0 30.5 31.0 31.5 32.00
5000000
10000000
15000000
20000000
0
500
1000
1500
2000
2500
3000
0
10000
20000
30000
40000
500000
2000
4000
6000
8000
10000
12000 NL: 1.21E4Base Peak m/z= 243.67-244.67 F: + c NSI SRM ms2 671.338 [244.165-244.167, 511.323-511.325, 755.454-755.456] MS IFIE_MP_0p5_S7_04sep08_Th_Kestrel_08-07-16_S7_2c
NL: 5.22E4Base Peak m/z= 510.82-511.82 F: + c NSI SRM ms2 671.338 [244.165-244.167, 511.323-511.325, 755.454-755.456] MS IFIE_MP_0p5_S7_04sep08_Th_Kestrel_08-07-16_S7_2c
NL: 3.34E3Base Peak m/z= 754.95-755.95 F: + c NSI SRM ms2 671.338 [244.165-244.167, 511.323-511.325, 755.454-755.456] MS IFIE_MP_0p5_S7_04sep08_Th_Kestrel_08-07-16_S7_2c
NL: 2.34E7Base Peak F: + c NSI SRM ms2 574.162 [397.248-397.250, 425.209-425.211] MS IFIE_MP_0p5_S7_04sep08_Th_Kestrel_08-07-16_S7_2c
RT: 26.62 - 31.50 SM: 15G
27.0 27.5 28.0 28.5 29.0 29.5 30.0 30.5 31.0 31.50
5000000
10000000
15000000
0
500
1000
1500
2000
2500
3000
3500
0
10000
20000
30000
40000
0
2000
4000
6000
8000
10000
NL: 1.17E4Base Peak m/z= 243.67-244.67 F: + c NSI SRM ms2 671.338 [244.165-244.167, 511.323-511.325, 755.454-755.456] MS IFIE_MP_0p5_S6_04sep08_Th_Kestrel_08-07-16_S6_2c
NL: 4.54E4Base Peak m/z= 510.82-511.82 F: + c NSI SRM ms2 671.338 [244.165-244.167, 511.323-511.325, 755.454-755.456] MS IFIE_MP_0p5_S6_04sep08_Th_Kestrel_08-07-16_S6_2c
NL: 3.94E3Base Peak m/z= 754.95-755.95 F: + c NSI SRM ms2 671.338 [244.165-244.167, 511.323-511.325, 755.454-755.456] MS IFIE_MP_0p5_S6_04sep08_Th_Kestrel_08-07-16_S6_2c
NL: 1.91E7Base Peak F: + c NSI SRM ms2 574.162 [397.248-397.250, 425.209-425.211] MS IFIE_MP_0p5_S6_04sep08_Th_Kestrel_08-07-16_S6_2c
40 µg/mL 4 µg/mL (LOD) 400 ng/mL
40 µg/mL 4 µg/mL (LOD) 400 ng/mL
a) Dual ion funnel interface
b) Standard Thermo interface
D: XICs of peptide LWSAEIPNLYR. LODs were determined for transition of m/z 681.362+ > 662.36+ . Renin substrate porcine(transition m/z 587.343+ > 696.30+) was used here as a retention time marker.
RT: 41.50 - 46.50 SM: 15G
41.5 42.0 42.5 43.0 43.5 44.0 44.5 45.0 45.5 46.0 46.50
50000000
100000000
150000000
200000000
0
2000000
4000000
6000000
8000000
10000000
12000000
140000000
1000000
2000000
3000000
4000000
50000000
2000000
4000000
6000000
8000000
10000000
12000000
14000000NL: 1.52E7Base Peak m/z= 661.86-662.86 F: + c NSI SRM ms2 681.364 [662.361-662.363, 775.445-775.447, 1062.557-1062.559] MS Genesis IFIE_MP_0p5_S9_22dec08_2F_Kestrel_08-11-06_S9_2c
NL: 5.27E6Base Peak m/z= 774.95-775.95 F: + c NSI SRM ms2 681.364 [662.361-662.363, 775.445-775.447, 1062.557-1062.559] MS IFIE_MP_0p5_S9_22dec08_2F_Kestrel_08-11-06_S9_2c
NL: 1.49E7Base Peak m/z= 1062.06-1063.06 F: + c NSI SRM ms2 681.364 [662.361-662.363, 775.445-775.447, 1062.557-1062.559] MS IFIE_MP_0p5_S9_22dec08_2F_Kestrel_08-11-06_S9_2c
NL: 2.29E8Base Peak F: + c NSI SRM ms2 587.340 [696.294-696.296, 745.888-745.890] MS IFIE_MP_0p5_S9_22dec08_2F_Kestrel_08-11-06_S9_2c
RT: 43.50 - 48.50 SM: 15G
43.5 44.0 44.5 45.0 45.5 46.0 46.5 47.0 47.5 48.0 48.50
50000000
100000000
150000000
200000000
2500000000
1000000
2000000
3000000
4000000
5000000
6000000
7000000
0
1000000
2000000
3000000
4000000
0
50000
100000
150000
200000
250000
300000
350000
400000 NL: 4.14E5Base Peak m/z= 661.86-662.86 F: + c NSI SRM ms2 681.364 [662.361-662.363, 775.445-775.447, 1062.557-1062.559] MS IFIE_MP_0p5_S8_22dec08_2F_Kestrel_08-11-06_S8_2b
NL: 4.29E6Base Peak m/z= 774.95-775.95 F: + c NSI SRM ms2 681.364 [662.361-662.363, 775.445-775.447, 1062.557-1062.559] MS IFIE_MP_0p5_S8_22dec08_2F_Kestrel_08-11-06_S8_2b
NL: 7.61E6Base Peak m/z= 1062.06-1063.06 F: + c NSI SRM ms2 681.364 [662.361-662.363, 775.445-775.447, 1062.557-1062.559] MS IFIE_MP_0p5_S8_22dec08_2F_Kestrel_08-11-06_S8_2b
NL: 2.54E8Base Peak F: + c NSI SRM ms2 587.340 [696.294-696.296, 745.888-745.890] MS IFIE_MP_0p5_S8_22dec08_2F_Kestrel_08-11-06_S8_2b
RT: 43.50 - 48.50 SM: 15G
43.5 44.0 44.5 45.0 45.5 46.0 46.5 47.0 47.5 48.0 48.50
50000000
100000000
150000000
200000000
0
1000000
2000000
3000000
4000000
5000000
6000000
70000000
500000
1000000
1500000
2000000
2500000
3000000
3500000
40000000
50000
100000
150000
200000NL: 2.21E5Base Peak m/z= 661.86-662.86 F: + c NSI SRM ms2 681.364 [662.361-662.363, 775.445-775.447, 1062.557-1062.559] MS IFIE_MP_0p5_S7_22dec08_2F_Kestrel_08-11-06_S7_2a
NL: 4.16E6Base Peak m/z= 774.95-775.95 F: + c NSI SRM ms2 681.364 [662.361-662.363, 775.445-775.447, 1062.557-1062.559] MS IFIE_MP_0p5_S7_22dec08_2F_Kestrel_08-11-06_S7_2a
NL: 7.41E6Base Peak m/z= 1062.06-1063.06 F: + c NSI SRM ms2 681.364 [662.361-662.363, 775.445-775.447, 1062.557-1062.559] MS IFIE_MP_0p5_S7_22dec08_2F_Kestrel_08-11-06_S7_2a
NL: 2.28E8Base Peak F: + c NSI SRM ms2 587.340 [696.294-696.296, 745.888-745.890] MS IFIE_MP_0p5_S7_22dec08_2F_Kestrel_08-11-06_S7_2a
RT: 39.50 - 44.50 SM: 15G
39.5 40.0 40.5 41.0 41.5 42.0 42.5 43.0 43.5 44.0 44.50
1000000
2000000
3000000
4000000
50000000
50000
100000
150000
2000000
20000
40000
60000
80000
100000
0
20000
40000
60000
80000
100000
120000
140000 NL: 1.44E5Base Peak m/z= 661.86-662.86 F: + c NSI SRM ms2 681.364 [662.361-662.363, 775.445-775.447, 1062.557-1062.559] MS IFIE_MP_0p5_S9_04sep08_Th_Kestrel_08-07-16_S9_2a
NL: 1.16E5Base Peak m/z= 774.95-775.95 F: + c NSI SRM ms2 681.364 [662.361-662.363, 775.445-775.447, 1062.557-1062.559] MS IFIE_MP_0p5_S9_04sep08_Th_Kestrel_08-07-16_S9_2a
NL: 2.13E5Base Peak m/z= 1062.06-1063.06 F: + c NSI SRM ms2 681.364 [662.361-662.363, 775.445-775.447, 1062.557-1062.559] MS IFIE_MP_0p5_S9_04sep08_Th_Kestrel_08-07-16_S9_2a
NL: 5.15E6Base Peak F: + c NSI SRM ms2 587.340 [696.294-696.296, 745.888-745.890] MS IFIE_MP_0p5_S9_04sep08_Th_Kestrel_08-07-16_S9_2a
RT: 37.50 - 42.50 SM: 15G
37.5 38.0 38.5 39.0 39.5 40.0 40.5 41.0 41.5 42.0 42.50
1000000
2000000
3000000
4000000
0
50000
100000
150000
0
20000
40000
60000
80000
0
10000
20000
30000
40000
50000
60000
70000NL: 7.36E4Base Peak m/z= 661.86-662.86 F: + c NSI SRM ms2 681.364 [662.361-662.363, 775.445-775.447, 1062.557-1062.559] MS IFIE_MP_0p5_S8_04sep08_Th_Kestrel_08-07-16_S8_2a
NL: 8.84E4Base Peak m/z= 774.95-775.95 F: + c NSI SRM ms2 681.364 [662.361-662.363, 775.445-775.447, 1062.557-1062.559] MS IFIE_MP_0p5_S8_04sep08_Th_Kestrel_08-07-16_S8_2a
NL: 1.74E5Base Peak m/z= 1062.06-1063.06 F: + c NSI SRM ms2 681.364 [662.361-662.363, 775.445-775.447, 1062.557-1062.559] MS IFIE_MP_0p5_S8_04sep08_Th_Kestrel_08-07-16_S8_2a
NL: 4.55E6Base Peak F: + c NSI SRM ms2 587.340 [696.294-696.296, 745.888-745.890] MS IFIE_MP_0p5_S8_04sep08_Th_Kestrel_08-07-16_S8_2a
RT: 37.50 - 43.50 SM: 15G
37.5 38.0 38.5 39.0 39.5 40.0 40.5 41.0 41.5 42.0 42.5 43.0 43.50
1000000
2000000
3000000
4000000
0
50000
100000
150000
0
20000
40000
60000
80000
1000000
10000
20000
30000
40000
50000NL: 5.30E4Base Peak m/z= 661.86-662.86 F: + c NSI SRM ms2 681.364 [662.361-662.363, 775.445-775.447, 1062.557-1062.559] MS IFIE_MP_0p5_S7_04sep08_Th_Kestrel_08-07-16_S7_2a
NL: 1.04E5Base Peak m/z= 774.95-775.95 F: + c NSI SRM ms2 681.364 [662.361-662.363, 775.445-775.447, 1062.557-1062.559] MS IFIE_MP_0p5_S7_04sep08_Th_Kestrel_08-07-16_S7_2a
NL: 2.00E5Base Peak m/z= 1062.06-1063.06 F: + c NSI SRM ms2 681.364 [662.361-662.363, 775.445-775.447, 1062.557-1062.559] MS IFIE_MP_0p5_S7_04sep08_Th_Kestrel_08-07-16_S7_2a
NL: 4.29E6Base Peak F: + c NSI SRM ms2 587.340 [696.294-696.296, 745.888-745.890] MS IFIE_MP_0p5_S7_04sep08_Th_Kestrel_08-07-16_S7_2a
40 µg/mL 400 ng/mL 80 ng/mL (LOD) 40 ng/mL
40 µg/mL 400 ng/mL 80 ng/mL (LOD) 40 ng/mL
a) Dual ion funnel interface
b) Standard Thermo interface
E: XICs of peptide LFTGHPETLEK. LODs were determined for transition of m/z 424.563+ > 506.26+ . Bradykinin fragment 1-7 (transition m/z 379.142+ > 527.31+) was used here as a retention time marker for accurate peak identification. On dual funnel interface,an interference between analyte peak of interest and a matrix peak confounded quantification of proteins at <80 ng/mL.
RT: 22.15 - 25.00 SM: 15G
22.5 23.0 23.5 24.0 24.5 25.00
20000000
40000000
60000000
80000000
100000000
120000000
1400000000
20000000
40000000
60000000
80000000
100000000
120000000
0
20000000
40000000
60000000
80000000
100000000
120000000
1400000000
50000000
100000000
150000000
200000000NL: 2.15E8Base Peak m/z= 505.76-506.76 F: + c NSI SRM ms2 424.559 [506.258-506.260, 579.792-579.794, 716.382-716.384] MS Genesis IFIE_MP_0p5_S9_22dec08_2F_Kestrel_08-11-06_S9_2a
NL: 1.45E8Base Peak m/z= 579.29-580.29 F: + c NSI SRM ms2 424.559 [506.258-506.260, 579.792-579.794, 716.382-716.384] MS IFIE_MP_0p5_S9_22dec08_2F_Kestrel_08-11-06_S9_2a
NL: 1.37E8Base Peak m/z= 715.88-716.88 F: + c NSI SRM ms2 424.559 [506.258-506.260, 579.792-579.794, 716.382-716.384] MS IFIE_MP_0p5_S9_22dec08_2F_Kestrel_08-11-06_S9_2a
NL: 1.47E8Base Peak F: + c NSI SRM ms2 379.142 [527.311-527.313, 614.355-614.357] MS IFIE_MP_0p5_S9_22dec08_2F_Kestrel_08-11-06_S9_2a
RT: 20.00 - 26.00 SM: 15G
20.0 20.5 21.0 21.5 22.0 22.5 23.0 23.5 24.0 24.5 25.0 25.5 26.00
1000000
2000000
3000000
4000000
5000000
6000000
0
200000
400000
600000
800000
1000000
1200000
1400000
16000000
500000
1000000
1500000
2000000
0
1000000
2000000
3000000
4000000
5000000NL: 5.25E6Base Peak m/z= 505.76-506.76 F: + c NSI SRM ms2 424.559 [506.258-506.260, 579.792-579.794, 716.382-716.384] MS IFIE_MP_0p5_S9_04sep08_Th_Kestrel_08-07-16_S9_2a
NL: 2.33E6Base Peak m/z= 579.29-580.29 F: + c NSI SRM ms2 424.559 [506.258-506.260, 579.792-579.794, 716.382-716.384] MS IFIE_MP_0p5_S9_04sep08_Th_Kestrel_08-07-16_S9_2a
NL: 1.61E6Base Peak m/z= 715.88-716.88 F: + c NSI SRM ms2 424.559 [506.258-506.260, 579.792-579.794, 716.382-716.384] MS IFIE_MP_0p5_S9_04sep08_Th_Kestrel_08-07-16_S9_2a
NL: 6.82E6Base Peak F: + c NSI SRM ms2 379.142 [527.311-527.313, 614.355-614.357] MS IFIE_MP_0p5_S9_04sep08_Th_Kestrel_08-07-16_S9_2a
RT: 18.00 - 24.00 SM: 15G
18.0 18.5 19.0 19.5 20.0 20.5 21.0 21.5 22.0 22.5 23.0 23.5 24.00
1000000
2000000
3000000
4000000
5000000
6000000
7000000
0
200000
400000
600000
800000
1000000
0
5000
10000
15000
200000
1000
2000
3000
4000
5000
6000
7000NL: 7.79E3Base Peak m/z= 505.76-506.76 F: + c NSI SRM ms2 424.559 [506.258-506.260, 579.792-579.794, 716.382-716.384] MS IFIE_MP_0p5_S6_04sep08_Th_Kestrel_08-07-16_S6_2a
NL: 2.12E4Base Peak m/z= 579.29-580.29 F: + c NSI SRM ms2 424.559 [506.258-506.260, 579.792-579.794, 716.382-716.384] MS IFIE_MP_0p5_S6_04sep08_Th_Kestrel_08-07-16_S6_2a
NL: 1.19E6Base Peak m/z= 715.88-716.88 F: + c NSI SRM ms2 424.559 [506.258-506.260, 579.792-579.794, 716.382-716.384] MS IFIE_MP_0p5_S6_04sep08_Th_Kestrel_08-07-16_S6_2a
NL: 7.89E6Base Peak F: + c NSI SRM ms2 379.142 [527.311-527.313, 614.355-614.357] MS IFIE_MP_0p5_S6_04sep08_Th_Kestrel_08-07-16_S6_2a
RT: 18.00 - 24.00 SM: 15G
18.0 18.5 19.0 19.5 20.0 20.5 21.0 21.5 22.0 22.5 23.0 23.5 24.00
2000000
4000000
6000000
8000000
0
200000
400000
600000
800000
1000000
1200000
0
50000
100000
150000
0
100000
200000
300000
400000
NL: 4.46E5Base Peak m/z= 505.76-506.76 F: + c NSI SRM ms2 424.559 [506.258-506.260, 579.792-579.794, 716.382-716.384] MS IFIE_MP_0p5_S8_04sep08_Th_Kestrel_08-07-16_S8_2a
NL: 1.92E5Base Peak m/z= 579.29-580.29 F: + c NSI SRM ms2 424.559 [506.258-506.260, 579.792-579.794, 716.382-716.384] MS IFIE_MP_0p5_S8_04sep08_Th_Kestrel_08-07-16_S8_2a
NL: 1.35E6Base Peak m/z= 715.88-716.88 F: + c NSI SRM ms2 424.559 [506.258-506.260, 579.792-579.794, 716.382-716.384] MS IFIE_MP_0p5_S8_04sep08_Th_Kestrel_08-07-16_S8_2a
NL: 8.97E6Base Peak F: + c NSI SRM ms2 379.142 [527.311-527.313, 614.355-614.357] MS IFIE_MP_0p5_S8_04sep08_Th_Kestrel_08-07-16_S8_2a
RT: 18.09 - 24.13 SM: 15G
18.5 19.0 19.5 20.0 20.5 21.0 21.5 22.0 22.5 23.0 23.5 24.00
1000000
2000000
3000000
4000000
5000000
6000000
7000000
0
500000
1000000
1500000
0
5000
10000
15000
0
1000
2000
3000
4000
5000
6000
7000 NL: 7.23E3Base Peak m/z= 505.76-506.76 F: + c NSI SRM ms2 424.559 [506.258-506.260, 579.792-579.794, 716.382-716.384] MS IFIE_MP_0p5_S5_04sep08_Th_Kestrel_08-07-16_S5_2a
NL: 1.74E4Base Peak m/z= 579.29-580.29 F: + c NSI SRM ms2 424.559 [506.258-506.260, 579.792-579.794, 716.382-716.384] MS IFIE_MP_0p5_S5_04sep08_Th_Kestrel_08-07-16_S5_2a
NL: 1.75E6Base Peak m/z= 715.88-716.88 F: + c NSI SRM ms2 424.559 [506.258-506.260, 579.792-579.794, 716.382-716.384] MS IFIE_MP_0p5_S5_04sep08_Th_Kestrel_08-07-16_S5_2a
NL: 7.71E6Base Peak F: + c NSI SRM ms2 379.142 [527.311-527.313, 614.355-614.357] MS IFIE_MP_0p5_S5_04sep08_Th_Kestrel_08-07-16_S5_2a
RT: 23.00 - 29.00 SM: 15G
23.0 23.5 24.0 24.5 25.0 25.5 26.0 26.5 27.0 27.5 28.0 28.5 29.00
50000000
100000000
150000000
0
5000000
10000000
15000000
20000000
25000000
30000000
35000000
400000000
100000
200000
300000
400000
500000
600000
700000
0
200000
400000
600000
800000
1000000
1200000
1400000 NL: 1.42E6Base Peak m/z= 505.76-506.76 F: + c NSI SRM ms2 424.559 [506.258-506.260, 579.792-579.794, 716.382-716.384] MS IFIE_MP_0p5_S7_22dec08_2F_Kestrel_08-11-06_S7_2b
NL: 7.74E5Base Peak m/z= 579.29-580.29 F: + c NSI SRM ms2 424.559 [506.258-506.260, 579.792-579.794, 716.382-716.384] MS IFIE_MP_0p5_S7_22dec08_2F_Kestrel_08-11-06_S7_2b
NL: 4.12E7Base Peak m/z= 715.88-716.88 F: + c NSI SRM ms2 424.559 [506.258-506.260, 579.792-579.794, 716.382-716.384] MS IFIE_MP_0p5_S7_22dec08_2F_Kestrel_08-11-06_S7_2b
NL: 1.89E8Base Peak F: + c NSI SRM ms2 379.142 [527.311-527.313, 614.355-614.357] MS IFIE_MP_0p5_S7_22dec08_2F_Kestrel_08-11-06_S7_2b
RT: 23.00 - 29.00 SM: 15G
23.0 23.5 24.0 24.5 25.0 25.5 26.0 26.5 27.0 27.5 28.0 28.5 29.00
20000000
40000000
60000000
80000000
100000000
120000000
140000000
1600000000
5000000
10000000
15000000
20000000
25000000
300000000
100000
200000
300000
400000
500000
6000000
100000
200000
300000
400000
500000
600000
700000NL: 7.30E5Base Peak m/z= 505.76-506.76 F: + c NSI SRM ms2 424.559 [506.258-506.260, 579.792-579.794, 716.382-716.384] MS IFIE_MP_0p5_S6_22dec08_2F_Kestrel_08-11-06_S6_2c
NL: 6.26E5Base Peak m/z= 579.29-580.29 F: + c NSI SRM ms2 424.559 [506.258-506.260, 579.792-579.794, 716.382-716.384] MS IFIE_MP_0p5_S6_22dec08_2F_Kestrel_08-11-06_S6_2c
NL: 3.18E7Base Peak m/z= 715.88-716.88 F: + c NSI SRM ms2 424.559 [506.258-506.260, 579.792-579.794, 716.382-716.384] MS IFIE_MP_0p5_S6_22dec08_2F_Kestrel_08-11-06_S6_2c
NL: 1.65E8Base Peak F: + c NSI SRM ms2 379.142 [527.311-527.313, 614.355-614.357] MS IFIE_MP_0p5_S6_22dec08_2F_Kestrel_08-11-06_S6_2c
RT: 21.50 - 26.00 SM: 15G
21.5 22.0 22.5 23.0 23.5 24.0 24.5 25.0 25.5 26.00
20000000
40000000
60000000
80000000
100000000
120000000
1400000000
5000000
10000000
15000000
20000000
25000000
30000000
350000000
200000
400000
600000
800000
1000000
1200000
0
100000
200000
300000
400000
500000
600000 NL: 6.17E5Base Peak m/z= 505.76-506.76 F: + c NSI SRM ms2 424.559 [506.258-506.260, 579.792-579.794, 716.382-716.384] MS IFIE_MP_0p5_S5_22dec08_2F_Kestrel_08-11-06_S5_2a
NL: 1.39E6Base Peak m/z= 579.29-580.29 F: + c NSI SRM ms2 424.559 [506.258-506.260, 579.792-579.794, 716.382-716.384] MS IFIE_MP_0p5_S5_22dec08_2F_Kestrel_08-11-06_S5_2a
NL: 3.68E7Base Peak m/z= 715.88-716.88 F: + c NSI SRM ms2 424.559 [506.258-506.260, 579.792-579.794, 716.382-716.384] MS IFIE_MP_0p5_S5_22dec08_2F_Kestrel_08-11-06_S5_2a
NL: 1.44E8Base Peak F: + c NSI SRM ms2 379.142 [527.311-527.313, 614.355-614.357] MS IFIE_MP_0p5_S5_22dec08_2F_Kestrel_08-11-06_S5_2a
40 µg/mL 4 µg/mL (LOD) 400 ng/mL
40 µg/mL (LOD) 4 µg/mL 400 ng/mL
a) Dual ion funnel interface
b) Standard Thermo interface
F: XICs of peptide YLEFISDAIIHVLHSK. LODs were determined for transition of m/z 629.013+ > 740.42+ . Renin substrate porcine (transition m/z 587.342+ > 696.30+) was used here as a retention time marker for accurate peak identification.
RT: 40.00 - 57.00 SM: 15G
40 42 44 46 48 50 52 54 560
50000000
100000000
150000000
200000000
0
2000000
4000000
6000000
8000000
10000000
12000000
14000000
160000000
1000000
2000000
3000000
4000000
5000000
0
500000
1000000
1500000
2000000
2500000
3000000NL: 3.13E6Base Peak m/z= 666.39-667.39 F: + c NSI SRM ms2 629.012 [666.884-666.886, 740.419-740.421, 804.940-804.942] MS IFIE_MP_0p5_S9_22dec08_2F_Kestrel_08-11-06_S9_2a
NL: 5.98E6Base Peak m/z= 739.92-740.92 F: + c NSI SRM ms2 629.012 [666.884-666.886, 740.419-740.421, 804.940-804.942] MS IFIE_MP_0p5_S9_22dec08_2F_Kestrel_08-11-06_S9_2a
NL: 1.61E7Base Peak m/z= 804.44-805.44 F: + c NSI SRM ms2 629.012 [666.884-666.886, 740.419-740.421, 804.940-804.942] MS IFIE_MP_0p5_S9_22dec08_2F_Kestrel_08-11-06_S9_2a
NL: 2.15E8Base Peak F: + c NSI SRM ms2 587.340 [696.294-696.296, 745.888-745.890] MS IFIE_MP_0p5_S9_22dec08_2F_Kestrel_08-11-06_S9_2a
RT: 45.39 - 64.48 SM: 15G
46 48 50 52 54 56 58 60 62 640
50000000
100000000
150000000
200000000
2500000000
100000
200000
300000
400000
0
20000
40000
60000
80000
100000
120000
140000
1600000
100000
200000
300000
400000NL: 4.22E5Base Peak m/z= 666.39-667.39 F: + c NSI SRM ms2 629.012 [666.884-666.886, 740.419-740.421, 804.940-804.942] MS IFIE_MP_0p5_S8_22dec08_2F_Kestrel_08-11-06_S8_2a
NL: 1.68E5Base Peak m/z= 739.92-740.92 F: + c NSI SRM ms2 629.012 [666.884-666.886, 740.419-740.421, 804.940-804.942] MS IFIE_MP_0p5_S8_22dec08_2F_Kestrel_08-11-06_S8_2a
NL: 4.62E5Base Peak m/z= 804.44-805.44 F: + c NSI SRM ms2 629.012 [666.884-666.886, 740.419-740.421, 804.940-804.942] MS IFIE_MP_0p5_S8_22dec08_2F_Kestrel_08-11-06_S8_2a
NL: 2.57E8Base Peak F: + c NSI SRM ms2 587.340 [696.294-696.296, 745.888-745.890] MS IFIE_MP_0p5_S8_22dec08_2F_Kestrel_08-11-06_S8_2a
RT: 45.00 - 63.00 SM: 15G
46 48 50 52 54 56 58 60 620
50000000
100000000
150000000
200000000
0
100000
200000
300000
400000
0
20000
40000
60000
80000
100000
120000
1400000
50000
100000
150000
200000
250000
300000
350000
400000 NL: 4.04E5Base Peak m/z= 666.39-667.39 F: + c NSI SRM ms2 629.012 [666.884-666.886, 740.419-740.421, 804.940-804.942] MS IFIE_MP_0p5_S7_22dec08_2F_Kestrel_08-11-06_S7_2a
NL: 1.47E5Base Peak m/z= 739.92-740.92 F: + c NSI SRM ms2 629.012 [666.884-666.886, 740.419-740.421, 804.940-804.942] MS IFIE_MP_0p5_S7_22dec08_2F_Kestrel_08-11-06_S7_2a
NL: 4.30E5Base Peak m/z= 804.44-805.44 F: + c NSI SRM ms2 629.012 [666.884-666.886, 740.419-740.421, 804.940-804.942] MS Genesis IFIE_MP_0p5_S7_22dec08_2F_Kestrel_08-11-06_S7_2a
NL: 2.28E8Base Peak F: + c NSI SRM ms2 587.340 [696.294-696.296, 745.888-745.890] MS IFIE_MP_0p5_S7_22dec08_2F_Kestrel_08-11-06_S7_2a
RT: 34.09 - 63.52 SM: 15G
36 38 40 42 44 46 48 50 52 54 56 58 60 620
500000
1000000
1500000
2000000
2500000
3000000
3500000
0
5000
10000
15000
20000
25000
30000
35000
0
2000
4000
6000
8000
10000
0
500
1000
1500
2000
NL: 2.24E3Base Peak m/z= 666.39-667.39 F: + c NSI SRM ms2 629.012 [666.884-666.886, 740.419-740.421, 804.940-804.942] MS IFIE_MP_0p5_S9_04sep08_Th_Kestrel_08-07-16_S9_2c
NL: 1.14E4Base Peak m/z= 739.92-740.92 F: + c NSI SRM ms2 629.012 [666.884-666.886, 740.419-740.421, 804.940-804.942] MS IFIE_MP_0p5_S9_04sep08_Th_Kestrel_08-07-16_S9_2c
NL: 3.92E4Base Peak m/z= 804.44-805.44 F: + c NSI SRM ms2 629.012 [666.884-666.886, 740.419-740.421, 804.940-804.942] MS IFIE_MP_0p5_S9_04sep08_Th_Kestrel_08-07-16_S9_2c
NL: 3.92E6Base Peak F: + c NSI SRM ms2 587.340 [696.294-696.296, 745.888-745.890] MS IFIE_MP_0p5_S9_04sep08_Th_Kestrel_08-07-16_S9_2c
RT: 39.98 - 58.31 SM: 15G
40 42 44 46 48 50 52 54 56 580
1000000
2000000
3000000
4000000
0
500
1000
1500
0
1000
2000
3000
4000
0
500
1000
1500
NL: 1.98E3Base Peak m/z= 666.39-667.39 F: + c NSI SRM ms2 629.012 [666.884-666.886, 740.419-740.421, 804.940-804.942] MS IFIE_MP_0p5_S8_04sep08_Th_Kestrel_08-07-16_S8_2c
NL: 4.35E3Base Peak m/z= 739.92-740.92 F: + c NSI SRM ms2 629.012 [666.884-666.886, 740.419-740.421, 804.940-804.942] MS IFIE_MP_0p5_S8_04sep08_Th_Kestrel_08-07-16_S8_2c
NL: 1.82E3Base Peak m/z= 804.44-805.44 F: + c NSI SRM ms2 629.012 [666.884-666.886, 740.419-740.421, 804.940-804.942] MS IFIE_MP_0p5_S8_04sep08_Th_Kestrel_08-07-16_S8_2c
NL: 4.29E6Base Peak F: + c NSI SRM ms2 587.340 [696.294-696.296, 745.888-745.890] MS IFIE_MP_0p5_S8_04sep08_Th_Kestrel_08-07-16_S8_2c
RT: 39.94 - 58.04 SM: 15G
40 42 44 46 48 50 52 54 56 580
1000000
2000000
3000000
4000000
0
200
400
600
800
1000
12000
1000
2000
3000
4000
5000
6000
0
500
1000
1500
NL: 1.89E3Base Peak m/z= 666.39-667.39 F: + c NSI SRM ms2 629.012 [666.884-666.886, 740.419-740.421, 804.940-804.942] MS IFIE_MP_0p5_S7_04sep08_Th_Kestrel_08-07-16_S7_2c
NL: 6.98E3Base Peak m/z= 739.92-740.92 F: + c NSI SRM ms2 629.012 [666.884-666.886, 740.419-740.421, 804.940-804.942] MS IFIE_MP_0p5_S7_04sep08_Th_Kestrel_08-07-16_S7_2c
NL: 1.21E3Base Peak m/z= 804.44-805.44 F: + c NSI SRM ms2 629.012 [666.884-666.886, 740.419-740.421, 804.940-804.942] MS IFIE_MP_0p5_S7_04sep08_Th_Kestrel_08-07-16_S7_2c
NL: 4.89E6Base Peak F: + c NSI SRM ms2 587.340 [696.294-696.296, 745.888-745.890] MS IFIE_MP_0p5_S7_04sep08_Th_Kestrel_08-07-16_S7_2c
40 µg/mL (LOD) 4 µg/mL 400 ng/mL
40 µg/mL (LOD) 4 µg/mL 400 ng/mL
a) Dual ion funnel interface
b) Standard Thermo interface
G: XICs of peptide HIATNAVLFFGR. LODs were determined for transition of m/z 673.372+ > 1095.59+ . [D-Ala-2]-Deltorphin II (transition m/z 783.192+ > 709.22+) was used here as a retention time marker.
RT: 34.67 - 39.07 SM: 15G
35.0 35.5 36.0 36.5 37.0 37.5 38.0 38.5 39.00
50000000
100000000
150000000
200000000
250000000
0
200000
400000
600000
800000
1000000
1200000
1400000
16000000
2000000
4000000
6000000
8000000
10000000
0
500000
1000000
1500000
2000000 NL: 2.03E6Base Peak m/z= 1024.06-1025.06 F: + c NSI SRM ms2 673.372 [1024.556-1024.558, 1095.594-1095.596, 1208.678-1208.680] MS IFIE_MP_0p5_S9_22dec08_2F_Kestrel_08-11-06_S9_2a
NL: 1.11E7Base Peak m/z= 1095.09-1096.09 F: + c NSI SRM ms2 673.372 [1024.556-1024.558, 1095.594-1095.596, 1208.678-1208.680] MS Genesis IFIE_MP_0p5_S9_22dec08_2F_Kestrel_08-11-06_S9_2a
NL: 1.65E6Base Peak m/z= 1208.18-1209.18 F: + c NSI SRM ms2 673.372 [1024.556-1024.558, 1095.594-1095.596, 1208.678-1208.680] MS IFIE_MP_0p5_S9_22dec08_2F_Kestrel_08-11-06_S9_2a
NL: 2.84E8Base Peak F: + c NSI SRM ms2 783.192 [610.175-610.177, 709.222-709.224] MS IFIE_MP_0p5_S9_22dec08_2F_Kestrel_08-11-06_S9_2a
RT: 34.67 - 40.07 SM: 15G
35.0 35.5 36.0 36.5 37.0 37.5 38.0 38.5 39.0 39.5 40.00
100000
200000
300000
4000000
5000
10000
15000
0
10000
20000
30000
40000
50000
60000
70000
0
10000
20000
30000
40000
NL: 4.60E4Base Peak m/z= 1024.06-1025.06 F: + c NSI SRM ms2 673.372 [1024.556-1024.558, 1095.594-1095.596, 1208.678-1208.680] MS Genesis IFIE_MP_0p5_S8_22dec08_2F_Kestrel_08-11-06_S8_2a
NL: 7.56E4Base Peak m/z= 1095.09-1096.09 F: + c NSI SRM ms2 673.372 [1024.556-1024.558, 1095.594-1095.596, 1208.678-1208.680] MS IFIE_MP_0p5_S8_22dec08_2F_Kestrel_08-11-06_S8_2a
NL: 1.91E4Base Peak m/z= 1208.18-1209.18 F: + c NSI SRM ms2 673.372 [1024.556-1024.558, 1095.594-1095.596, 1208.678-1208.680] MS IFIE_MP_0p5_S8_22dec08_2F_Kestrel_08-11-06_S8_2a
NL: 4.23E5Base Peak F: + c NSI SRM ms2 783.192 [610.175-610.177, 709.222-709.224] MS IFIE_MP_0p5_S8_22dec08_2F_Kestrel_08-11-06_S8_2a
RT: 34.67 - 40.07 SM: 15G
35.0 35.5 36.0 36.5 37.0 37.5 38.0 38.5 39.0 39.5 40.00
50000
100000
150000
200000
250000
300000
350000
0
5000
10000
15000
0
5000
10000
15000
20000
25000
30000
0
5000
10000
15000
20000
25000
30000
35000
NL: 3.96E4Base Peak m/z= 1024.06-1025.06 F: + c NSI SRM ms2 673.372 [1024.556-1024.558, 1095.594-1095.596, 1208.678-1208.680] MS Genesis IFIE_MP_0p5_S7_22dec08_2F_Kestrel_08-11-06_S7_2a
NL: 3.44E4Base Peak m/z= 1095.09-1096.09 F: + c NSI SRM ms2 673.372 [1024.556-1024.558, 1095.594-1095.596, 1208.678-1208.680] MS IFIE_MP_0p5_S7_22dec08_2F_Kestrel_08-11-06_S7_2a
NL: 1.87E4Base Peak m/z= 1208.18-1209.18 F: + c NSI SRM ms2 673.372 [1024.556-1024.558, 1095.594-1095.596, 1208.678-1208.680] MS IFIE_MP_0p5_S7_22dec08_2F_Kestrel_08-11-06_S7_2a
NL: 3.81E5Base Peak F: + c NSI SRM ms2 783.192 [610.175-610.177, 709.222-709.224] MS IFIE_MP_0p5_S7_22dec08_2F_Kestrel_08-11-06_S7_2a
RT: 33.00 - 38.00 SM: 15G
33.0 33.5 34.0 34.5 35.0 35.5 36.0 36.5 37.0 37.5 38.00
1000000
2000000
3000000
4000000
0
20
40
60
80
100
120
1400
1000
2000
3000
4000
5000
6000
70000
200
400
600
800NL: 8.93E2Base Peak m/z= 1024.06-1025.06 F: + c NSI SRM ms2 673.372 [1024.556-1024.558, 1095.594-1095.596, 1208.678-1208.680] MS IFIE_MP_0p5_S9_04sep08_Th_Kestrel_08-07-16_S9_2c
NL: 7.25E3Base Peak m/z= 1095.09-1096.09 F: + c NSI SRM ms2 673.372 [1024.556-1024.558, 1095.594-1095.596, 1208.678-1208.680] MS Genesis IFIE_MP_0p5_S9_04sep08_Th_Kestrel_08-07-16_S9_2c
NL: 1.48E2Base Peak m/z= 1208.18-1209.18 F: + c NSI SRM ms2 673.372 [1024.556-1024.558, 1095.594-1095.596, 1208.678-1208.680] MS IFIE_MP_0p5_S9_04sep08_Th_Kestrel_08-07-16_S9_2c
NL: 4.80E6Base Peak F: + c NSI SRM ms2 783.192 [610.175-610.177, 709.222-709.224] MS IFIE_MP_0p5_S9_04sep08_Th_Kestrel_08-07-16_S9_2c
RT: 34.50 - 39.00 SM: 15G
34.5 35.0 35.5 36.0 36.5 37.0 37.5 38.0 38.5 39.00
500000
1000000
1500000
2000000
2500000
3000000
3500000
40000000
100
200
300
400
500
600
0
100
200
300
400
0
200
400
600
800
NL: 9.72E2Base Peak m/z= 1024.06-1025.06 F: + c NSI SRM ms2 673.372 [1024.556-1024.558, 1095.594-1095.596, 1208.678-1208.680] MS Genesis IFIE_MP_0p5_S8_04sep08_Th_Kestrel_08-07-16_S8_2c
NL: 4.45E2Base Peak m/z= 1095.09-1096.09 F: + c NSI SRM ms2 673.372 [1024.556-1024.558, 1095.594-1095.596, 1208.678-1208.680] MS IFIE_MP_0p5_S8_04sep08_Th_Kestrel_08-07-16_S8_2c
NL: 6.56E2Base Peak m/z= 1208.18-1209.18 F: + c NSI SRM ms2 673.372 [1024.556-1024.558, 1095.594-1095.596, 1208.678-1208.680] MS IFIE_MP_0p5_S8_04sep08_Th_Kestrel_08-07-16_S8_2c
NL: 4.21E6Base Peak F: + c NSI SRM ms2 783.192 [610.175-610.177, 709.222-709.224] MS IFIE_MP_0p5_S8_04sep08_Th_Kestrel_08-07-16_S8_2c
RT: 33.50 - 38.00 SM: 15G
33.5 34.0 34.5 35.0 35.5 36.0 36.5 37.0 37.5 38.00
500000
1000000
1500000
2000000
2500000
3000000
3500000
0
10
20
30
40
0
10
20
30
40
50
60
700
100
200
300
400
NL: 4.48E2Base Peak m/z= 1024.06-1025.06 F: + c NSI SRM ms2 673.372 [1024.556-1024.558, 1095.594-1095.596, 1208.678-1208.680] MS IFIE_MP_0p5_S7_04sep08_Th_Kestrel_08-07-16_S7_2c
NL: 7.06E1Base Peak m/z= 1095.09-1096.09 F: + c NSI SRM ms2 673.372 [1024.556-1024.558, 1095.594-1095.596, 1208.678-1208.680] MS Genesis IFIE_MP_0p5_S7_04sep08_Th_Kestrel_08-07-16_S7_2c
NL: 4.53E1Base Peak m/z= 1208.18-1209.18 F: + c NSI SRM ms2 673.372 [1024.556-1024.558, 1095.594-1095.596, 1208.678-1208.680] MS IFIE_MP_0p5_S7_04sep08_Th_Kestrel_08-07-16_S7_2c
NL: 3.99E6Base Peak F: + c NSI SRM ms2 783.192 [610.175-610.177, 709.222-709.224] MS IFIE_MP_0p5_S7_04sep08_Th_Kestrel_08-07-16_S7_2c
40 µg/mL 400 ng/mL 80 ng/mL (LOD) 40 ng/mL
40 µg/mL 4 µg/mL (LOD) 400 ng/mL
a) Dual ion funnel interface
b) Standard Thermo interface
H: XICs of peptide GGLEPINFQTAADQAR. LODs were determined for transition of m/z 844.422+ > 666.34+ . Methionine enkephalin (transition m/z 574.162+ > 397.25+) was used here as a retention time marker .
RT: 33.20 - 36.79 SM: 15G
33.5 34.0 34.5 35.0 35.5 36.0 36.50
50000000
100000000
150000000
200000000
250000000
0
2000000
4000000
6000000
8000000
0
1000000
2000000
3000000
4000000
5000000
0
5000000
10000000
15000000
20000000
NL: 2.27E7Base Peak m/z= 665.84-666.84 F: + c NSI SRM ms2 844.424 [666.338-666.340, 732.363-732.365, 1331.669-1331.671] MS Genesis IFIE_MP_0p5_S9_22dec08_2F_Kestrel_08-11-06_S9_2c
NL: 6.00E6Base Peak m/z= 731.86-732.86 F: + c NSI SRM ms2 844.424 [666.338-666.340, 732.363-732.365, 1331.669-1331.671] MS IFIE_MP_0p5_S9_22dec08_2F_Kestrel_08-11-06_S9_2c
NL: 8.58E6Base Peak m/z= 1331.17-1332.17 F: + c NSI SRM ms2 844.424 [666.338-666.340, 732.363-732.365, 1331.669-1331.671] MS IFIE_MP_0p5_S9_22dec08_2F_Kestrel_08-11-06_S9_2c
NL: 2.91E8Base Peak F: + c NSI SRM ms2 574.162 [397.248-397.250, 425.209-425.211] MS IFIE_MP_0p5_S9_22dec08_2F_Kestrel_08-11-06_S9_2c
RT: 35.36 - 38.81 SM: 15G
35.5 36.0 36.5 37.0 37.5 38.0 38.50
50000000
100000000
150000000
200000000
250000000
0
5000
10000
15000
200000
10000
20000
30000
40000
50000
0
20000
40000
60000
80000
100000NL: 1.09E5Base Peak m/z= 665.84-666.84 F: + c NSI SRM ms2 844.424 [666.338-666.340, 732.363-732.365, 1331.669-1331.671] MS IFIE_MP_0p5_S7_22dec08_2F_Kestrel_08-11-06_S7_2c
NL: 5.70E4Base Peak m/z= 731.86-732.86 F: + c NSI SRM ms2 844.424 [666.338-666.340, 732.363-732.365, 1331.669-1331.671] MS IFIE_MP_0p5_S7_22dec08_2F_Kestrel_08-11-06_S7_2c
NL: 2.06E4Base Peak m/z= 1331.17-1332.17 F: + c NSI SRM ms2 844.424 [666.338-666.340, 732.363-732.365, 1331.669-1331.671] MS IFIE_MP_0p5_S7_22dec08_2F_Kestrel_08-11-06_S7_2c
NL: 2.91E8Base Peak F: + c NSI SRM ms2 574.162 [397.248-397.250, 425.209-425.211] MS IFIE_MP_0p5_S7_22dec08_2F_Kestrel_08-11-06_S7_2c
RT: 34.37 - 37.50 SM: 15G
34.5 35.0 35.5 36.0 36.5 37.0 37.50
50000000
100000000
150000000
200000000
250000000
0
500
1000
1500
2000
2500
3000
3500
40000
10000
20000
30000
40000
50000
60000
0
20000
40000
60000
80000
NL: 9.44E4Base Peak m/z= 665.84-666.84 F: + c NSI SRM ms2 844.424 [666.338-666.340, 732.363-732.365, 1331.669-1331.671] MS IFIE_MP_0p5_S6_22dec08_2F_Kestrel_08-11-06_S6_2c
NL: 6.45E4Base Peak m/z= 731.86-732.86 F: + c NSI SRM ms2 844.424 [666.338-666.340, 732.363-732.365, 1331.669-1331.671] MS IFIE_MP_0p5_S6_22dec08_2F_Kestrel_08-11-06_S6_2c
NL: 4.20E3Base Peak m/z= 1331.17-1332.17 F: + c NSI SRM ms2 844.424 [666.338-666.340, 732.363-732.365, 1331.669-1331.671] MS IFIE_MP_0p5_S6_22dec08_2F_Kestrel_08-11-06_S6_2c
NL: 2.91E8Base Peak F: + c NSI SRM ms2 574.162 [397.248-397.250, 425.209-425.211] MS IFIE_MP_0p5_S6_22dec08_2F_Kestrel_08-11-06_S6_2c
RT: 31.99 - 35.12 SM: 15G
32.0 32.5 33.0 33.5 34.0 34.5 35.00
50000000
100000000
150000000
200000000
250000000
0
1000
2000
3000
4000
5000
6000
70000
5000
10000
15000
20000
25000
300000
10000
20000
30000
40000
50000
60000NL: 6.28E4Base Peak m/z= 665.84-666.84 F: + c NSI SRM ms2 844.424 [666.338-666.340, 732.363-732.365, 1331.669-1331.671] MS Genesis IFIE_MP_0p5_S5_22dec08_2F_Kestrel_08-11-06_S5_2a
NL: 3.11E4Base Peak m/z= 731.86-732.86 F: + c NSI SRM ms2 844.424 [666.338-666.340, 732.363-732.365, 1331.669-1331.671] MS IFIE_MP_0p5_S5_22dec08_2F_Kestrel_08-11-06_S5_2a
NL: 7.01E3Base Peak m/z= 1331.17-1332.17 F: + c NSI SRM ms2 844.424 [666.338-666.340, 732.363-732.365, 1331.669-1331.671] MS IFIE_MP_0p5_S5_22dec08_2F_Kestrel_08-11-06_S5_2a
NL: 2.91E8Base Peak F: + c NSI SRM ms2 574.162 [397.248-397.250, 425.209-425.211] MS IFIE_MP_0p5_S5_22dec08_2F_Kestrel_08-11-06_S5_2a
RT: 28.59 - 35.06 SM: 15G
29.0 29.5 30.0 30.5 31.0 31.5 32.0 32.5 33.0 33.5 34.0 34.5 35.00
2000000
4000000
6000000
8000000
10000000
0
10000
20000
30000
40000
50000
60000
70000
0
20000
40000
60000
80000
100000
0
50000
100000
150000
200000 NL: 2.05E5Base Peak m/z= 665.84-666.84 F: + c NSI SRM ms2 844.424 [666.338-666.340, 732.363-732.365, 1331.669-1331.671] MS Genesis IFIE_MP_0p5_S9_04sep08_Th_Kestrel_08-07-16_S9_2c
NL: 1.12E5Base Peak m/z= 731.86-732.86 F: + c NSI SRM ms2 844.424 [666.338-666.340, 732.363-732.365, 1331.669-1331.671] MS IFIE_MP_0p5_S9_04sep08_Th_Kestrel_08-07-16_S9_2c
NL: 7.58E4Base Peak m/z= 1331.17-1332.17 F: + c NSI SRM ms2 844.424 [666.338-666.340, 732.363-732.365, 1331.669-1331.671] MS IFIE_MP_0p5_S9_04sep08_Th_Kestrel_08-07-16_S9_2c
NL: 1.19E7Base Peak F: + c NSI SRM ms2 574.162 [397.248-397.250, 425.209-425.211] MS IFIE_MP_0p5_S9_04sep08_Th_Kestrel_08-07-16_S9_2c
RT: 29.39 - 35.60 SM: 15G
29.5 30.0 30.5 31.0 31.5 32.0 32.5 33.0 33.5 34.0 34.5 35.0 35.50
5000000
10000000
15000000
200000000
1000
2000
3000
4000
5000
0
20000
40000
60000
80000
100000
1200000
2000
4000
6000
8000
10000
12000
NL: 1.35E4Base Peak m/z= 665.84-666.84 F: + c NSI SRM ms2 844.424 [666.338-666.340, 732.363-732.365, 1331.669-1331.671] MS IFIE_MP_0p5_S8_04sep08_Th_Kestrel_08-07-16_S8_2c
NL: 1.24E5Base Peak m/z= 731.86-732.86 F: + c NSI SRM ms2 844.424 [666.338-666.340, 732.363-732.365, 1331.669-1331.671] MS IFIE_MP_0p5_S8_04sep08_Th_Kestrel_08-07-16_S8_2c
NL: 5.77E3Base Peak m/z= 1331.17-1332.17 F: + c NSI SRM ms2 844.424 [666.338-666.340, 732.363-732.365, 1331.669-1331.671] MS IFIE_MP_0p5_S8_04sep08_Th_Kestrel_08-07-16_S8_2c
NL: 2.14E7Base Peak F: + c NSI SRM ms2 574.162 [397.248-397.250, 425.209-425.211] MS IFIE_MP_0p5_S8_04sep08_Th_Kestrel_08-07-16_S8_2c
RT: 29.38 - 35.63 SM: 15G
29.5 30.0 30.5 31.0 31.5 32.0 32.5 33.0 33.5 34.0 34.5 35.0 35.50
5000000
10000000
15000000
20000000
0
100
200
300
400
500
600
700
0
20000
40000
60000
80000
100000
120000
1400000
2000
4000
6000
8000
10000NL: 1.09E4Base Peak m/z= 665.84-666.84 F: + c NSI SRM ms2 844.424 [666.338-666.340, 732.363-732.365, 1331.669-1331.671] MS IFIE_MP_0p5_S7_04sep08_Th_Kestrel_08-07-16_S7_2c
NL: 1.44E5Base Peak m/z= 731.86-732.86 F: + c NSI SRM ms2 844.424 [666.338-666.340, 732.363-732.365, 1331.669-1331.671] MS IFIE_MP_0p5_S7_04sep08_Th_Kestrel_08-07-16_S7_2c
NL: 7.62E2Base Peak m/z= 1331.17-1332.17 F: + c NSI SRM ms2 844.424 [666.338-666.340, 732.363-732.365, 1331.669-1331.671] MS IFIE_MP_0p5_S7_04sep08_Th_Kestrel_08-07-16_S7_2c
NL: 2.34E7Base Peak F: + c NSI SRM ms2 574.162 [397.248-397.250, 425.209-425.211] MS IFIE_MP_0p5_S7_04sep08_Th_Kestrel_08-07-16_S7_2c
40 µg/mL 400 ng/mL 80 ng/mL (LOD) 40 ng/mL
40 µg/mL 4 µg/mL 400 ng/mL (LOD) 80 ng/mL
a) Dual ion funnel interface
b) Standard Thermo interface
I: XICs of peptide EDLIAYLK. LODs were determined for transition of m/z 482.772+ > 494.30+ . [D-Ala-2]-Deltorphin II (transition m/z 783.192+ > 709.22+) was used here as a retention time marker.
RT: 35.50 - 41.00
35.5 36.0 36.5 37.0 37.5 38.0 38.5 39.0 39.5 40.0 40.5 41.00
1000000
2000000
3000000
4000000
5000000
0
20000
40000
60000
80000
100000
120000
1400000
50000
100000
150000
200000
250000
300000
0
200000
400000
600000
800000
1000000NL: 1.06E6Base Peak m/z= 493.80-494.80 F: + c NSI SRM ms2 482.771 [494.296-494.298, 607.380-607.382, 720.464-720.466] MS Genesis IFIE_MP_0p5_S9_04sep08_Th_Kestrel_08-07-16_S9_2a
NL: 3.32E5Base Peak m/z= 606.88-607.88 F: + c NSI SRM ms2 482.771 [494.296-494.298, 607.380-607.382, 720.464-720.466] MS IFIE_MP_0p5_S9_04sep08_Th_Kestrel_08-07-16_S9_2a
NL: 1.41E5Base Peak m/z= 719.97-720.97 F: + c NSI SRM ms2 482.771 [494.296-494.298, 607.380-607.382, 720.464-720.466] MS IFIE_MP_0p5_S9_04sep08_Th_Kestrel_08-07-16_S9_2a
NL: 5.65E6Base Peak F: + c NSI SRM ms2 783.192 [610.175-610.177, 709.222-709.224] MS IFIE_MP_0p5_S9_04sep08_Th_Kestrel_08-07-16_S9_2a
RT: 34.00 - 39.00
34.0 34.5 35.0 35.5 36.0 36.5 37.0 37.5 38.0 38.5 39.00
1000000
2000000
3000000
4000000
0
5000
10000
15000
0
5000
10000
15000
20000
0
20000
40000
60000
80000
100000NL: 1.10E5Base Peak m/z= 493.80-494.80 F: + c NSI SRM ms2 482.771 [494.296-494.298, 607.380-607.382, 720.464-720.466] MS Genesis IFIE_MP_0p5_S8_04sep08_Th_Kestrel_08-07-16_S8_2a
NL: 2.23E4Base Peak m/z= 606.88-607.88 F: + c NSI SRM ms2 482.771 [494.296-494.298, 607.380-607.382, 720.464-720.466] MS IFIE_MP_0p5_S8_04sep08_Th_Kestrel_08-07-16_S8_2a
NL: 1.75E4Base Peak m/z= 719.97-720.97 F: + c NSI SRM ms2 482.771 [494.296-494.298, 607.380-607.382, 720.464-720.466] MS IFIE_MP_0p5_S8_04sep08_Th_Kestrel_08-07-16_S8_2a
NL: 4.57E6Base Peak F: + c NSI SRM ms2 783.192 [610.175-610.177, 709.222-709.224] MS IFIE_MP_0p5_S8_04sep08_Th_Kestrel_08-07-16_S8_2a
RT: 33.50 - 39.00
33.5 34.0 34.5 35.0 35.5 36.0 36.5 37.0 37.5 38.0 38.5 39.00
1000000
2000000
3000000
4000000
0
500
1000
1500
0
1000
2000
3000
4000
5000
6000
70000
2000
4000
6000
8000
10000
12000
14000NL: 1.51E4Base Peak m/z= 493.80-494.80 F: + c NSI SRM ms2 482.771 [494.296-494.298, 607.380-607.382, 720.464-720.466] MS IFIE_MP_0p5_S7_04sep08_Th_Kestrel_08-07-16_S7_2a
NL: 7.05E3Base Peak m/z= 606.88-607.88 F: + c NSI SRM ms2 482.771 [494.296-494.298, 607.380-607.382, 720.464-720.466] MS IFIE_MP_0p5_S7_04sep08_Th_Kestrel_08-07-16_S7_2a
NL: 1.81E3Base Peak m/z= 719.97-720.97 F: + c NSI SRM ms2 482.771 [494.296-494.298, 607.380-607.382, 720.464-720.466] MS IFIE_MP_0p5_S7_04sep08_Th_Kestrel_08-07-16_S7_2a
NL: 4.79E6Base Peak F: + c NSI SRM ms2 783.192 [610.175-610.177, 709.222-709.224] MS IFIE_MP_0p5_S7_04sep08_Th_Kestrel_08-07-16_S7_2a
RT: 33.00 - 39.00
33.0 33.5 34.0 34.5 35.0 35.5 36.0 36.5 37.0 37.5 38.0 38.5 39.00
1000000
2000000
3000000
4000000
0
50
100
150
0
1000
2000
3000
4000
50000
1000
2000
3000
4000
NL: 4.73E3Base Peak m/z= 493.80-494.80 F: + c NSI SRM ms2 482.771 [494.296-494.298, 607.380-607.382, 720.464-720.466] MS IFIE_MP_0p5_S6_04sep08_Th_Kestrel_08-07-16_S6_2a
NL: 5.01E3Base Peak m/z= 606.88-607.88 F: + c NSI SRM ms2 482.771 [494.296-494.298, 607.380-607.382, 720.464-720.466] MS IFIE_MP_0p5_S6_04sep08_Th_Kestrel_08-07-16_S6_2a
NL: 1.86E2Base Peak m/z= 719.97-720.97 F: + c NSI SRM ms2 482.771 [494.296-494.298, 607.380-607.382, 720.464-720.466] MS IFIE_MP_0p5_S6_04sep08_Th_Kestrel_08-07-16_S6_2a
NL: 4.47E6Base Peak F: + c NSI SRM ms2 783.192 [610.175-610.177, 709.222-709.224] MS IFIE_MP_0p5_S6_04sep08_Th_Kestrel_08-07-16_S6_2a
RT: 37.00 - 43.00
37.0 37.5 38.0 38.5 39.0 39.5 40.0 40.5 41.0 41.5 42.0 42.5 43.00
50000000
100000000
150000000
200000000
250000000
0
1000000
2000000
3000000
4000000
0
2000000
4000000
6000000
8000000
10000000
12000000
140000000
5000000
10000000
15000000
20000000
25000000
30000000
35000000
40000000 NL: 4.05E7Base Peak m/z= 493.80-494.80 F: + c NSI SRM ms2 482.771 [494.296-494.298, 607.380-607.382, 720.464-720.466] MS Genesis IFIE_MP_0p5_S9_22dec08_2F_Kestrel_08-11-06_S9_2c
NL: 1.43E7Base Peak m/z= 606.88-607.88 F: + c NSI SRM ms2 482.771 [494.296-494.298, 607.380-607.382, 720.464-720.466] MS IFIE_MP_0p5_S9_22dec08_2F_Kestrel_08-11-06_S9_2c
NL: 4.57E6Base Peak m/z= 719.97-720.97 F: + c NSI SRM ms2 482.771 [494.296-494.298, 607.380-607.382, 720.464-720.466] MS IFIE_MP_0p5_S9_22dec08_2F_Kestrel_08-11-06_S9_2c
NL: 2.86E8Base Peak F: + c NSI SRM ms2 783.192 [610.175-610.177, 709.222-709.224] MS IFIE_MP_0p5_S9_22dec08_2F_Kestrel_08-11-06_S9_2c
RT: 40.00 - 46.00
40.0 40.5 41.0 41.5 42.0 42.5 43.0 43.5 44.0 44.5 45.0 45.5 46.00
50000000
100000000
150000000
200000000
250000000
0
50000
100000
150000
200000
2500000
100000
200000
300000
400000
0
50000
100000
150000
200000
250000
300000
NL: 3.48E5Base Peak m/z= 493.80-494.80 F: + c NSI SRM ms2 482.771 [494.296-494.298, 607.380-607.382, 720.464-720.466] MS IFIE_MP_0p5_S7_22dec08_2F_Kestrel_08-11-06_S7_2c
NL: 4.63E5Base Peak m/z= 606.88-607.88 F: + c NSI SRM ms2 482.771 [494.296-494.298, 607.380-607.382, 720.464-720.466] MS IFIE_MP_0p5_S7_22dec08_2F_Kestrel_08-11-06_S7_2c
NL: 2.50E5Base Peak m/z= 719.97-720.97 F: + c NSI SRM ms2 482.771 [494.296-494.298, 607.380-607.382, 720.464-720.466] MS IFIE_MP_0p5_S7_22dec08_2F_Kestrel_08-11-06_S7_2c
NL: 2.86E8Base Peak F: + c NSI SRM ms2 783.192 [610.175-610.177, 709.222-709.224] MS IFIE_MP_0p5_S7_22dec08_2F_Kestrel_08-11-06_S7_2c
RT: 38.50 - 45.00
38.5 39.0 39.5 40.0 40.5 41.0 41.5 42.0 42.5 43.0 43.5 44.0 44.5 45.00
50000000
100000000
150000000
200000000
250000000
0
50000
100000
150000
200000
250000
3000000
100000
200000
300000
400000
0
20000
40000
60000
80000
100000
120000
140000
160000NL: 1.68E5Base Peak m/z= 493.80-494.80 F: + c NSI SRM ms2 482.771 [494.296-494.298, 607.380-607.382, 720.464-720.466] MS IFIE_MP_0p5_S6_22dec08_2F_Kestrel_08-11-06_S6_2c
NL: 4.86E5Base Peak m/z= 606.88-607.88 F: + c NSI SRM ms2 482.771 [494.296-494.298, 607.380-607.382, 720.464-720.466] MS IFIE_MP_0p5_S6_22dec08_2F_Kestrel_08-11-06_S6_2c
NL: 3.14E5Base Peak m/z= 719.97-720.97 F: + c NSI SRM ms2 482.771 [494.296-494.298, 607.380-607.382, 720.464-720.466] MS IFIE_MP_0p5_S6_22dec08_2F_Kestrel_08-11-06_S6_2c
NL: 2.86E8Base Peak F: + c NSI SRM ms2 783.192 [610.175-610.177, 709.222-709.224] MS IFIE_MP_0p5_S6_22dec08_2F_Kestrel_08-11-06_S6_2c
RT: 36.50 - 41.50
36.5 37.0 37.5 38.0 38.5 39.0 39.5 40.0 40.5 41.0 41.50
50000000
100000000
150000000
200000000
250000000
0
20000
40000
60000
80000
100000
120000
140000
1600000
50000
100000
150000
200000
2500000
20000
40000
60000
80000
100000
120000 NL: 1.22E5Base Peak m/z= 493.80-494.80 F: + c NSI SRM ms2 482.771 [494.296-494.298, 607.380-607.382, 720.464-720.466] MS IFIE_MP_0p5_S5_22dec08_2F_Kestrel_08-11-06_S5_2a
NL: 2.58E5Base Peak m/z= 606.88-607.88 F: + c NSI SRM ms2 482.771 [494.296-494.298, 607.380-607.382, 720.464-720.466] MS IFIE_MP_0p5_S5_22dec08_2F_Kestrel_08-11-06_S5_2a
NL: 1.64E5Base Peak m/z= 719.97-720.97 F: + c NSI SRM ms2 482.771 [494.296-494.298, 607.380-607.382, 720.464-720.466] MS IFIE_MP_0p5_S5_22dec08_2F_Kestrel_08-11-06_S5_2a
NL: 2.85E8Base Peak F: + c NSI SRM ms2 783.192 [610.175-610.177, 709.222-709.224] MS IFIE_MP_0p5_S5_22dec08_2F_Kestrel_08-11-06_S5_2a
40 µg/mL 80 ng/mL 40 ng/mL (LOD) 8 ng/mL
40 µg/mL 4 µg/mL 400 ng/mL (LOD) 80 ng/mL
a) Dual ion funnel interface
b) Standard Thermo interface
J: XICs of peptide TGQAPGFSYTDANK. LODs were determined for transition of m/z 728.842+ > 1099.51+ . Bradykinin fragment 1-7 (transition m/z 379.142+ > 614.36+) was used here as a retention time marker.
RT: 20.00 - 26.00 SM: 15G
20.0 20.5 21.0 21.5 22.0 22.5 23.0 23.5 24.0 24.5 25.0 25.5 26.00
1000000
2000000
3000000
4000000
5000000
6000000
0
100000
200000
300000
400000
0
10000
20000
30000
40000
50000
60000
70000
0
50000
100000
150000
200000
250000NL: 2.71E5Base Peak m/z= 357.67-358.67 F: + c NSI SRM ms2 728.839 [358.171-358.173, 550.255-550.257, 1099.505-1099.507] MS IFIE_MP_0p5_S9_04sep08_Th_Kestrel_08-07-16_S9_2a
NL: 7.63E4Base Peak m/z= 549.76-550.76 F: + c NSI SRM ms2 728.839 [358.171-358.173, 550.255-550.257, 1099.505-1099.507] MS IFIE_MP_0p5_S9_04sep08_Th_Kestrel_08-07-16_S9_2a
NL: 4.75E5Base Peak m/z= 1099.01-1100.01 F: + c NSI SRM ms2 728.839 [358.171-358.173, 550.255-550.257, 1099.505-1099.507] MS Genesis IFIE_MP_0p5_S9_04sep08_Th_Kestrel_08-07-16_S9_2a
NL: 6.82E6Base Peak F: + c NSI SRM ms2 379.142 [527.311-527.313, 614.355-614.357] MS IFIE_MP_0p5_S9_04sep08_Th_Kestrel_08-07-16_S9_2a
RT: 19.50 - 24.50 SM: 15G
19.5 20.0 20.5 21.0 21.5 22.0 22.5 23.0 23.5 24.0 24.50
2000000
4000000
6000000
8000000
0
5000
10000
15000
20000
25000
30000
35000
0
1000
2000
3000
4000
5000
6000
7000
0
5000
10000
15000
NL: 1.96E4Base Peak m/z= 357.67-358.67 F: + c NSI SRM ms2 728.839 [358.171-358.173, 550.255-550.257, 1099.505-1099.507] MS IFIE_MP_0p5_S8_04sep08_Th_Kestrel_08-07-16_S8_2a
NL: 7.76E3Base Peak m/z= 549.76-550.76 F: + c NSI SRM ms2 728.839 [358.171-358.173, 550.255-550.257, 1099.505-1099.507] MS IFIE_MP_0p5_S8_04sep08_Th_Kestrel_08-07-16_S8_2a
NL: 3.94E4Base Peak m/z= 1099.01-1100.01 F: + c NSI SRM ms2 728.839 [358.171-358.173, 550.255-550.257, 1099.505-1099.507] MS Genesis IFIE_MP_0p5_S8_04sep08_Th_Kestrel_08-07-16_S8_2a
NL: 8.97E6Base Peak F: + c NSI SRM ms2 379.142 [527.311-527.313, 614.355-614.357] MS IFIE_MP_0p5_S8_04sep08_Th_Kestrel_08-07-16_S8_2a
RT: 19.00 - 25.00 SM: 15G
19.0 19.5 20.0 20.5 21.0 21.5 22.0 22.5 23.0 23.5 24.0 24.5 25.00
1000000
2000000
3000000
4000000
5000000
6000000
7000000
0
500
1000
1500
2000
0
200
400
600
800
1000
1200
14000
50
100
150
200
250
300
350NL: 3.67E2Base Peak m/z= 357.67-358.67 F: + c NSI SRM ms2 728.839 [358.171-358.173, 550.255-550.257, 1099.505-1099.507] MS Genesis IFIE_MP_0p5_S7_04sep08_Th_Kestrel_08-07-16_S7_2a
NL: 1.45E3Base Peak m/z= 549.76-550.76 F: + c NSI SRM ms2 728.839 [358.171-358.173, 550.255-550.257, 1099.505-1099.507] MS IFIE_MP_0p5_S7_04sep08_Th_Kestrel_08-07-16_S7_2a
NL: 2.46E3Base Peak m/z= 1099.01-1100.01 F: + c NSI SRM ms2 728.839 [358.171-358.173, 550.255-550.257, 1099.505-1099.507] MS IFIE_MP_0p5_S7_04sep08_Th_Kestrel_08-07-16_S7_2a
NL: 7.95E6Base Peak F: + c NSI SRM ms2 379.142 [527.311-527.313, 614.355-614.357] MS IFIE_MP_0p5_S7_04sep08_Th_Kestrel_08-07-16_S7_2a
RT: 18.75 - 24.75 SM: 15G
19.0 19.5 20.0 20.5 21.0 21.5 22.0 22.5 23.0 23.5 24.0 24.50
1000000
2000000
3000000
4000000
5000000
6000000
7000000
0
100
200
300
400
500
0
100
200
300
400
500
0
100
200
300
400
500
600
NL: 6.78E2Base Peak m/z= 357.67-358.67 F: + c NSI SRM ms2 728.839 [358.171-358.173, 550.255-550.257, 1099.505-1099.507] MS IFIE_MP_0p5_S6_04sep08_Th_Kestrel_08-07-16_S6_2a
NL: 5.72E2Base Peak m/z= 549.76-550.76 F: + c NSI SRM ms2 728.839 [358.171-358.173, 550.255-550.257, 1099.505-1099.507] MS IFIE_MP_0p5_S6_04sep08_Th_Kestrel_08-07-16_S6_2a
NL: 5.76E2Base Peak m/z= 1099.01-1100.01 F: + c NSI SRM ms2 728.839 [358.171-358.173, 550.255-550.257, 1099.505-1099.507] MS IFIE_MP_0p5_S6_04sep08_Th_Kestrel_08-07-16_S6_2a
NL: 7.89E6Base Peak F: + c NSI SRM ms2 379.142 [527.311-527.313, 614.355-614.357] MS IFIE_MP_0p5_S6_04sep08_Th_Kestrel_08-07-16_S6_2a
RT: 19.30 - 28.31 SM: 15G
20 21 22 23 24 25 26 27 280
20000000
40000000
60000000
80000000
100000000
120000000
1400000000
5000000
10000000
15000000
20000000
25000000
30000000
35000000
400000000
2000000
4000000
6000000
8000000
0
5000000
10000000
15000000
NL: 1.96E7Base Peak m/z= 357.67-358.67 F: + c NSI SRM ms2 728.839 [358.171-358.173, 550.255-550.257, 1099.505-1099.507] MS IFIE_MP_0p5_S9_22dec08_2F_Kestrel_08-11-06_S9_2a
NL: 8.61E6Base Peak m/z= 549.76-550.76 F: + c NSI SRM ms2 728.839 [358.171-358.173, 550.255-550.257, 1099.505-1099.507] MS IFIE_MP_0p5_S9_22dec08_2F_Kestrel_08-11-06_S9_2a
NL: 4.13E7Base Peak m/z= 1099.01-1100.01 F: + c NSI SRM ms2 728.839 [358.171-358.173, 550.255-550.257, 1099.505-1099.507] MS IFIE_MP_0p5_S9_22dec08_2F_Kestrel_08-11-06_S9_2a
NL: 1.47E8Base Peak F: + c NSI SRM ms2 379.142 [527.311-527.313, 614.355-614.357] MS IFIE_MP_0p5_S9_22dec08_2F_Kestrel_08-11-06_S9_2a
RT: 22.30 - 30.31 SM: 15G
23 24 25 26 27 28 29 300
20000000
40000000
60000000
80000000
100000000
120000000
140000000
1600000000
10000
20000
30000
40000
50000
0
50000
100000
150000
2000000
10000
20000
30000
40000
NL: 4.77E4Base Peak m/z= 357.67-358.67 F: + c NSI SRM ms2 728.839 [358.171-358.173, 550.255-550.257, 1099.505-1099.507] MS IFIE_MP_0p5_S6_22dec08_2F_Kestrel_08-11-06_S6_2a
NL: 2.07E5Base Peak m/z= 549.76-550.76 F: + c NSI SRM ms2 728.839 [358.171-358.173, 550.255-550.257, 1099.505-1099.507] MS IFIE_MP_0p5_S6_22dec08_2F_Kestrel_08-11-06_S6_2a
NL: 5.70E4Base Peak m/z= 1099.01-1100.01 F: + c NSI SRM ms2 728.839 [358.171-358.173, 550.255-550.257, 1099.505-1099.507] MS IFIE_MP_0p5_S6_22dec08_2F_Kestrel_08-11-06_S6_2a
NL: 1.62E8Base Peak F: + c NSI SRM ms2 379.142 [527.311-527.313, 614.355-614.357] MS IFIE_MP_0p5_S6_22dec08_2F_Kestrel_08-11-06_S6_2a
RT: 19.30 - 28.31 SM: 15G
20 21 22 23 24 25 26 27 280
20000000
40000000
60000000
80000000
100000000
120000000
1400000000
5000
10000
15000
20000
25000
0
20000
40000
60000
80000
100000
120000
140000
0
100000
200000
300000
400000
NL: 4.67E5Base Peak m/z= 357.67-358.67 F: + c NSI SRM ms2 728.839 [358.171-358.173, 550.255-550.257, 1099.505-1099.507] MS IFIE_MP_0p5_S5_22dec08_2F_Kestrel_08-11-06_S5_2a
NL: 1.50E5Base Peak m/z= 549.76-550.76 F: + c NSI SRM ms2 728.839 [358.171-358.173, 550.255-550.257, 1099.505-1099.507] MS IFIE_MP_0p5_S5_22dec08_2F_Kestrel_08-11-06_S5_2a
NL: 2.84E4Base Peak m/z= 1099.01-1100.01 F: + c NSI SRM ms2 728.839 [358.171-358.173, 550.255-550.257, 1099.505-1099.507] MS IFIE_MP_0p5_S5_22dec08_2F_Kestrel_08-11-06_S5_2a
NL: 1.44E8Base Peak F: + c NSI SRM ms2 379.142 [527.311-527.313, 614.355-614.357] MS IFIE_MP_0p5_S5_22dec08_2F_Kestrel_08-11-06_S5_2a
RT: 20.30 - 29.31 SM: 15G
21 22 23 24 25 26 27 28 290
20000000
40000000
60000000
80000000
100000000
120000000
0
2000
4000
6000
8000
10000
12000
140000
50000
100000
150000
0
5000
10000
15000
20000
25000
30000
35000NL: 3.67E4Base Peak m/z= 357.67-358.67 F: + c NSI SRM ms2 728.839 [358.171-358.173, 550.255-550.257, 1099.505-1099.507] MS IFIE_MP_0p5_S4_22dec08_2F_Kestrel_08-11-06_S4_2a
NL: 1.88E5Base Peak m/z= 549.76-550.76 F: + c NSI SRM ms2 728.839 [358.171-358.173, 550.255-550.257, 1099.505-1099.507] MS IFIE_MP_0p5_S4_22dec08_2F_Kestrel_08-11-06_S4_2a
NL: 1.49E4Base Peak m/z= 1099.01-1100.01 F: + c NSI SRM ms2 728.839 [358.171-358.173, 550.255-550.257, 1099.505-1099.507] MS IFIE_MP_0p5_S4_22dec08_2F_Kestrel_08-11-06_S4_2a
NL: 1.40E8Base Peak F: + c NSI SRM ms2 379.142 [527.311-527.313, 614.355-614.357] MS IFIE_MP_0p5_S4_22dec08_2F_Kestrel_08-11-06_S4_2a
