Supplemental Figure 3
description
Transcript of Supplemental Figure 3
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Supplemental Figure 3
Effects of -GlcY and -GalY on the expression of -GlcY-inducible genes, GA-inducible genes and repressors of GA signaling. Real-time RT-PCR and semi-quantitative RT-PCR were performed using cDNA from 1.5 g total RNA, which were the same RNA samples as used in Fig. 5, isolated from 2.5 x 105 protoplasts. Aleurone protoplasts were treated with DMSO as a control or GA3 plus -GalY or GA3 plus -GlcY for 24 hours. (A) -GlcY-inducible genes, (B) GA-inducible genes, (C) repressors of GA signaling. In real-time RT-PCR analysis, data shown are transcript levels relative to mock-treated samples (control = 100) and ubiquitin-conjugating enzyme (Contig4868_at) was used as an internal standard. In semi-quantitative RT-PCR analysis, GAPDH (Contig149_at) and PolyA-binding protein (Contig546_at) genes were chosen for internal controls. The primers used in this study are listed in Supplemental Table 5.
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0
200
400
600
800
1000
1200
1400
1600
1800
2000
24 Cont 24 GA+alpha 24 GA+beta
Chitinase III
Blue copper-binding protein
Oxalate oxidase
Permatin PR5
GAPDH
polyA-binding protein
A
0
200
400
600
800
1000
24 Cont 24 GA+alpha 24 GA+beta
Ferritin
0
200
400
600
800
24 Cont 24 GA+alpha 24 GA+beta
0
500
1000
1500
2000
2500
24 Cont 24 GA+alpha 24 GA+beta
Amy32b
Pirin
0
40
80
120
160
24 Cont 24 GA+alpha 24 GA+beta
HvWRKY38
0
40
80
120
160
24 Cont 24 GA+alpha 24 GA+beta
0
50
100
150
200
250
300
350
24 Cont 24 GA+alpha 24 GA+beta
FAD-linked oxidoreductase
0
50
100
150
200
250
24 Cont 24 GA+alpha 24 GA+beta
0
50
100
150
200
250
300
350
400
24 Cont 24 GA+alpha 24 GA+beta
0
1000
2000
3000
4000
5000
6000
24 Cont 24 GA+alpha 24 GA+beta
EPB1
Chitinase class I
HvBWMK1
Hypothetical protein
GAPDH
polyA-binding protein
B
HvWRKY51 HvEsi47C
Re
lativ
e t
ran
scrip
t le
vel
(tra
nsc
ript
of
con
tro
l = 1
00
)Cont -GalY -GlcY
+GA3
Rel
ativ
e tr
ansc
ript l
evel
(t
rans
crip
t of c
ontr
ol =
100
)
Cont -GalY -GlcY
+GA3
Rel
ativ
e tr
ansc
ript l
evel
(t
rans
crip
t of c
ontr
ol =
100
)
Cont -GalY -GlcY
+GA3
Cont -GalY -GlcY
+GA3
Cont -GalY -GlcY
+GA3
Cont -GalY -GlcY
+GA3
Cont -GalY -GlcY
+GA3
Cont -GalY -GlcY
+GA3
Cont -GalY -GlcY
+GA3