Streptomyces Bio Remediation Activity

8/7/2019 Streptomyces Bio Remediation Activity

1/14

1.1.1.1.BioReLab

BioReLab

BioReLab

BioReLab.,DepartmentofZ

oology/Entomology,HNBGU

(ACentralUniversity),Cam

pusBadshahithaulTehri

.,DepartmentofZ




.,DepartmentofZ




.,DepartmentofZ



pusBadshahithaulTehri----24

9199(UK)

24

9199(UK)

24

9199(UK)

24

9199(UK)

2.DepartmentofZoology,GovernmentPGCollege,



2.DepartmentofZoology,GovernmentPGCollege,Kotdwara

Kotdwara

Kotdwara

KotdwaraGa

rhwal

Ga

rhwal

Ga

rhwal

Ga

rhwal(UK)INDIA

(UK)INDIA

(UK)INDIA

(UK)INDIA

1.

Correspondingauthor:aso

[email protected]:+91

9450501471



9450501471



9450501471



9450501471


2/14

INT

RODUCT

ION

Inrecentyears,

Uttarakhandhas

emergedason

eofthemostattractive

industrialdestinationsinIndia.

TheGovernment

isencouragingpr

ivateparticipationinallindustrial

activities

andasaresultbig

playerssuchasHLLandDaburhave

setupunitsinthestate.

placetheregulato

ryframeworkforUttarakhand'sindustrialization.

TheNewIndustrialPolicyindicates

thatprivateresou

rcesmaybetapp

edwhile

promotingintegra

tedIndustrialEsta

tesinUttarakhand.

TheindustrialsectoroftheUttarakhandstategrowin

gdaybydayand

itisvery

goodforthedeve

lopmentofstate

andcountryalso,butweshouldre

member

thesecondphage

ofindustrialization.

Itistheindustria

leffluent,dispose

dbytheindustries.Theindustrial

effluents

havemanypolluta

ntsincludingheavymetals.


3/14

HEA

VY

META

L

POLLUTION

Inearlydaysofab

undantresources

andnegligiblede

velopmentpressu

res,little

attentionwaspaidtoenvironmentalissue,although

someenvironmen

trelated

legislationpertain

ingtodifferentsectorswasauthoriz

ed.

Rapideconomicc

hangeshaveresu

ltedinelevatedleveloftoxicheav

ymetals

andradionuclides

enteringthebiosphere.

So

an

or

qu

wastescontan

ngtoxc

eavymetasmay

egenerate

n

variousindustrialprocessessuch

aschemicalmanufacturing,electricpower

generating,coala

ndoremining,sm

ellingandmetal

refining,metalplatingand

others.

Continuousmonitoringofheavy

metalslevelin

theenvironment

isvery

importantsinceitcannotbedegradedandbecomingpublichealth

problem

whenincreasedaboveacceptancelevel.

Healthproblemd

uetoheavymeta

lspollutionincludenausea,vomiting,bone

complications,ne

rvoussystem

imp

airmentsandevendeathbecome

amajor

problem

throughoutmanycountrieswhenmetalionsconcentratio

ninthe

environmentexceededtheadmissib

lelimits.


4/14

ME

THODOLOGY

Sam

pleCollectionan

dProcessing:

Theliquidandsolidwastes

werecollectedfromd

ifferentsitesofUttarakhandstateincluding

Sevageand

Municipality(APHA199

8).Theleachatefromsolidwastewasprepared

accordingtothemetho

ddescribed

byFrenchStandardmethod(Ferrarietal.,1999

,Srivastavaetal.,2005

andSavithaetal.,2010

).Themetal

concentrationsanalysed

byICP-MS(Ashoketal

2010).

Mic

robialstrainand

HeavymetalTra

ining:

Streptomycessp.strainsweremaintainedinagarsla

nts(Holtetal1994)co

ntainingnutrientbroth

,whichwas

isolatedfrom

sludgecontaminatedwithheav

ymetal.Individualstockmetalionsolutions

ofdifferent

concentrationofCufromCuSO4.5H2O,Cdfrom

3CdSO4.8H2O,Crfrom

K2Cr2O7,NifromNiCl.6H

2O,Pbfrom

PbacetateandZnfrom

ZnSO4respectivelywasprepared.(Hammainietal2006).Microbialstrainswere

allowedtogrowinthesupplementedmediawithmetalconcentration,

whichwasincreased0

g/mlto350

g/ml(Kumaretal2010b).

Bios

orptionExperim

ent:

Exper

imentsofheavymetals

biosorptionweredoneinErlenmeyerflaskscon

taining150mland15.0

1.0mgof

cells.Toensureequilib

rium,cellsandwasteweremaintainedincontactfor48hours,und

erconstant

agitation,at30-350C

2C.After48hours,cellswereseparatedfro

m

themedium

andresidualmetal

concentrationsweremo

nitoredbyICP-MS.The

optimumpHandtempe

raturemaintainedforth

egrowthof

microorganismsintheb

atchculture.(Chatarzee

etal2001;Cybulskieta

l,2003;Kumaretal201

0b).


5/14

RESU

LTS

ToleranceofMic

robes

Theculturescondition

s:Temperature(2830oC),pHof6.8

0.2.

Themetalconcentration-0mg/l(control

)to300mg/l

StreptomycesspshowedthetoleranceforCrandco-tolera

nceforPbandCu.

The

controlandoptimizationofbiorem

ediationprocess

esisacomplexs

ystem

of

.

Existenceofa

microbialsps.cap

ableofdegrading

thepollutants

availabilityof

contaminantstot

hemicrobialpopu

lation

Environmentfactorssuchastem

perature

pHPresenceofoxygenorotherele

ctronacceptors

Nutrients

Micr

obes

were

allowed

to

grown

in

the

media

containing

heavy

metals

concentrationand

theywereadopt

edfortheconcernedmetals.Understanding

metalmicroberelationshipshasled

toadvancesinbioremediation(Ma

lik2004;

Bruinsetal.2000)

.


6/14

METAL

TOLERA

NCE

6

0

7

0

8

0

Streptomycess

p.

Biosorption

capa

cities

dependon-

Metal

concentration

pH

of

the

solut

ion

Contacttime

Ion

icstrength

Presence

of

competitiveions.

MorleyandGadd

70

5

5

25

15

10

5

0

1

0

2

0

3

0

4

0

5

0

C

2

5

50

100

200

250

No.ofColonie

Concentrationofm

etal(mg/l)

(1995

)concluded

for

fungal

biom

assthatthe

differentcellwall

polym

ers

have

vario

usfunctional

groups

and

differing

charge

distributions

and

there

fore

different

metal-

bindingcapacities

anda

ffinities.


7/14

BIOR

EMEDIATION

ACTIVITY

Thre

emetalCu,CrandPdweresorbedbyStreptomy

cessp.

Copperreductionrangedfrom0.288

-1.129mg/l,chromiumreduction

ranged

from0.386-6.42mg/l,whichshow

edmaximumre

ductioninchrom

iumby

Streptomycessp

andreductionin

leadrangedfrom0.063-0.286m

g/l.

Sha

rma

and

Loya

l(2009)

studied

the

chrom

ium

(III)

removalby

Streptomycessp

fromtanneryef

fluentandfound

thesimilarresu

ltsasin

caseofchromium

removal.

Pura

nik,etal.(1995)foundStrepto

mycespimprina

waspotentbio

sorbent

forcadmiuminh

isstudy.

Stre

ptomycesrimos

usbiomassused

forCu,Znand

Crbiosorptionbymany

investigators(Ad

dour,etal.199

9;Chergui,eta

l.2007;Ashwini,etal.

2009).


8/14

ACT

IVITY

OF

STREPTOMYCES

SP

Site

Sample

CM(Cu)

CMF(Cu)

CM(Cr

)

CMF(Cr)

CM(Pb)

CMF(Pb)

HR

Solid*

ND

ND

2.09

0.564

ND

ND

Liquid

3.90

3.198

8.56

2.140

0.858

0.582

DD

Solid

ND

ND

3.34

0.935

ND

ND

Table

1:

Metal

conc.inthewaste

samp

les(CM

and

CMF)

.

Optimal

cond

itions:

Liquid

4.78

3.872

1.85

0.536

0.560

0.380

MR

Solid

ND

ND

0.56

0.174

ND

ND

Liquid

5.95

4.821

1.05

0.294

0.202

0.139

RK

Solid

ND

ND

0.96

0.288

ND

ND

Liquid

1.80

1.512

1.45

0.405

0.865

0.579

KR

Solid

ND

ND

3.12

0.845

ND

ND

Liquid

ND

ND

2.15

0.602

0.564

0.383

RR

Solid

ND

ND

3.45

0.935

ND

ND

Liquid

4.78

3.858

ND

ND

0.562

0.382

Temp.

30C

30C

and

pH

6.8

0.2.

Samplewereof

sixcities:

HR:

Haridwar

DD:

Dehradun

MR:

Mossorrie

RK:

Rishikesh

KR:

Kashipur

RR:

Roorkee


9/14

BIOACC

UMULAT

ION

68

Cu

Cr

Pb

Graph

2:

Bioaccumulatio

n

(mg/g

of

Biom

ass)

by

Strep

tomyces

sp

(mul

tiresistant).

Ashok

Kumar

024

They

reduced

three

metals

viz.Cr,Cuand

Pb.

Max.reduction

recorded

by

acclimated

species

was

chromium,

it

was72%.


10/14

DISCUSS

ION

Microorganisms

areintimatelyinvolvedinmetalbiogeochemistrywitha

varietyofproces

sesdetermining

mobilityandtherefore,bioavailability.

Thebalancebetweenmobilizationandimmobilizationvariesde

pending

on

theorganismsinvolved,th

eirenvironmen

tand

physicoc

hemical

Ashok

Kumar

.

Metal

mobilization

can

ar

ise

from

a

variety

of

leaching

mechanisms,complexationby

metabolites

and

siderophores

and

methylation,wh

erethisresultsinvolatilization.Inaddition,redu

ctionof

highervalencyspeciesmayeffectmobilization,e.g.,Cr(VI)toCr(III).

Inthecontexto

fbioremediatio

n,solubilization

ofmetalconta

minants

provides

am

eans

of

remo

val

from

solid

matrices,s

uch

as

soils,sediments,dumpsandothersolidindustrialwastes(Kum

aretal

2010).


11/14

C

ONCLUS

ION

The

tolerancetest

indicatedthatamongexper

imentalheavy

metals

maximum

tole

rancewassho

wntoCr,Pb

andCd,showingthe

growthofStrep

tomycesspup

to200-250ug/

ml.

The

heavymetals

reducedbyfilamentousbac

teriaStreptom

ycessp

arecopper,chromiumandlead.

The

averageCure

ductionbyStreptomycesspw

as18%,Crreduction

wasrecordeda

s72%andPbreductionwas32%.

The

gram

positive

bacteriaStre

ptomycesspw

asprovedas

strong

biosorbentforchromiumheav

ymetal.

Furtherstudiesshouldbecarried

outtoestablishthequantityorlevel

ofbioremediationwhichaffec

tsthetreatmen

tefficiency.


12/14

Authorsaret

hankfultoProf.J.P.Bhatt,HOD

Biotechnology

and

Zoo

logy,

HNBG

U

(A

central

Un

iversity)

S

rinagar

Ga

rhwal

(Uttarakhand

)

INDIAf

or

his

va

luable

Acknowle

dgements

suggesonsa

n

r.

aurav

upa,

recor

HimachalInstituteofLife

Sciences,Pa

nonta

Sahib

(HP)

INDIA

forprovidingth

e

lab

facility.

Theauthors

arealsogratefultoD

r.A.K

Singh,

Scien

tist

C,

Wadia

Institute

of

HimalayanGeologyforv

aluablecom

ments

andtechnical

support.


13/14

SNAP

SHOTS

..


14/14

T

Y

Ashok

Kumar

f

y

e

Forfurtherquer

iesrelatedtoBiore

mediation:[email protected]

OrContactat+919450501471

Streptomyces Bio Remediation Activity

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