Effectiveness of Blumea balsamifera (sambong)

As tea against Escherichia coli

A science investigatory project

In partial fulfillment for Science

Investigatory project

From the class of

Mr. Denis Dyvee Errabo

Submitted by:

Llido, Danice Y.

Diaz, Xena Nicole P.

Del Carmen, Denice

Ordoveza, Geneva Mae

Espinosa, Marielen

Fedelis, Jay Paul

Cantangco, Jesrick

October 7, 2014

ACKNOWLEDGEMENT

The researchers acknowledge the person who give importance for this

investigation and also a person whose valuable experience and for those people who

help to make this investigatory project together:

First of all the researchers give thanks for our JESUS CHRIST SON OF GOD

For those wisdom and knowledge that He gave to make this project perfectly by the

God’s purpose, by the strength and blessings to be part of this project and by proving

that all things are possible by His name and also for His generous personality. PRAISE

THE LORD that the researchers receive it all from Him;

And for the researcher’s PARENTS who totally support in some activities of the

school also for this kind of experimentation and who has been there along their journey

also giving encouragement and inspiration.

And for the TEACHERS of researcher who also understand when the researcher

came to school lately, and also giving inspiration and giving strength and when each of

them strive for the researchers happiness at the back of stressful projects.

To their loving FRIENDS who cheer up along the journey and also proposing

hope when the things seemed to be impossible.

THE RESEARCHERS

DEDICATION

The RESEARCHERS dedicate it for those people who have been there in times

of needs and also along their journey.

And for the researcher’s supportive PARENTS who help when no one is around

to make this project together with other researchers.

And Mr. Denis Dyvee Errabo who persists to make this project and give

authority to study about this given priority.

Lastly our FATHER GOD holy in His purpose and proving that the researchers

can do this before the submission, who also guides the researchers to make possible

the impossible.

ABSTRACT

The researchers want to conduct an investigatory project that is all about

concentration that can kill microorganisms. The researchers want to create a product

that can kill the E. coli that cause diarrhea. The researchers want to prove that the

Blumea balsamifera (Sambong) is effective in curing diarrhea or killing E. coli. The

importance of this study is to help the people needed medicine for healing, maintenance

of health, activity, body comfortable and conditioning. The researchers use the Blumea

balsamifera (Sambong) as the main ingredient of the product. Blumea balsamifera

(Sambong) is the one of the common anti-microbial herbal medicine to prevent

Escherichia coli. The researchers start doing this investigatory project on August 5,

2014 during the rainy season where the diarrhea is prone.

The researchers hypothesized that Blumea balsamifera (sambong) is effective in

killing the Escherichia coli. In 1997, the Traditional and Alternative Medicine Acts

(TAMA) was passed and it became legal to sell these plants for medicinal purposes in

the Philippines. Sambong tea is also used for herbal treatment of diarrhea and stomach

spasms. A Photochemical study indicates that sambong leaves contains the chemicals

icthyothereol acetate, cyptomeridiol, lutein and ß-carotene that has been found to have

positive activity against microbes such as a Niger, T mentagrophytes and C albicans.

Results also showed activity against P aeruginosa, S aureus, B subtilis and E coli.

The researchers tested their extract in Natural Science Research Institute (NSRI)

in University of the Philippines, to test the effectiveness of the Blumea balsamifera to

the Escherichia coli. The researchers use the leaves of Blumea balsamifera as the main

subject of the research and used 100 % concentration. Microbial suspension was

prepared from 24-hour old culture of the test organism. The suspending medium use

was 0.1% peptone water. Pre-poured Nutrient Agar (NA) plates, about 3 mm thick, were

inoculated with the microbial suspension by swabbing the agar surface. The cotton on

swab on an applicator stick was dipped into the microbial suspension, rotated several

time and pressed firmly on the inside wall of the tube above the fluid level to remove

excess inoculums from the swab. The swab was streaked over the entire agar surface.

This procedure was repeated in two more times, rotating the plate 60 degree each time

to ensure even distribution of the inoculums. Three equidistant wells were on the agar

plate using a cork bore (10mm diameter). Two hundred (200) ml of the sample was

placed in each well. For the positive control, a chloramphenicol disc was placed on the

center of the agar plate. It incubated at 35 degree Celsius and observed 24 hours.

Based on the findings the extracted Blumea balsamifera can’t kill the

microorganisms in diarrhea such as Escherichia coli either during the examination the

extracted sambong is contaminated. Therefore, the researchers aiming that they can do

this in a perfect way although there’s a possibility to fail just to prove that this leaves are

effective as an alternative to prevent the diarrhea or stomach cramps.

TABLE OF CONTENTS

Page

Approval Sheet i

Acknowledgement ii

Dedication iii

Abstract iv

Chapter I: Review of Related Literature

Introduction ………………………………………………………. 1-2

Background of the Study ……………………………………….. 3-4

Scope and Limitation ……………………………………………. 5

Statement of the Problem ………………………………………. 6

Chapter II: Evaluation Design and Framework

Discussion of expected Output

And Justification …………….………………………………….. 7

Theoretical Framework …………………………………………. 8

Conceptual Framework ………………………………………… 9

The Variables, The Definitions and

Importance to the Study ……………………………………….. 10-11

Definition of Terms …………………………………………….. 12

Chapter III: Research Methodology

Research Design ……………………………………………… 13

Setting of the Study …………………………………………... 14

Subject of the Study ……………………………………………. 14

Procedure of the Study ………………………………………… 15

Sources of Data ………………………………………………… 16

Statistical Treatment …………………………………………… 17

Chapter IV: Result and Discussion

Presentation of data, analysis

And Interpretation of Data …………………………………… 18

Chapter V: Summary, Conclusion and Recommendation

Summary …………………………………………………………. 19

Conclusion ………………………………………………………. 19

Recommendation ………………………………………………. 19

Chapter IV: End Matters

Bibliography ……………………………………………………. 20

Curriculum Vitae ……………………………………………….. 21

CHAPTER I

INTRODUCTION

Nowadays, diarrheal diseases remain a leading cause of preventable

death, especially among children under five in developing countries. The normal

intestinal tract regulates the absorption and secretion of electrolytes and water to meet

the body's physiological needs. More than 98 percent of the 10 liters per day of fluid

entering the adult intestines are reabsorbed. The remaining stool water, related

primarily to the indigestible fiber content, determines the consistency of normal feces

from dry, hard pellets to mushy, bulky stools, varying from person to person, day to day,

and stool to stool. This variation complicates the definition of diarrhea, which by

convention is present when three or more stools are passed in 24 hours that are

sufficiently liquid to take the shape of the container in which they are placed.

Diarrhea is prone when the country is encountering rainy days. Traditionally, our

ancestors used tea as an herbal medicine in curing diarrhea. Tea is a substance that

can be use to lessen the stomach pain, in our country diarrhea greatly spread,

according to the study the most prone on it are the marginalized family in this case they

are immune to eat contaminated food one of this familiar terms are the pagpag which

can cause diarrhea. Although tea is common to prevent or to lessen the stomach ache

the researchers continue to study about it, because of a higher formulation of the people

who totally involve in this said disease.

The Sambong (Blumea balsamifera) is now registered and approved by the Food

and Drug Administration as a medicine. This includes the researchers to have a strong

determination to kill the microorganism Escherichia coli (E.coli) that actively cause of

diarrhea. The researchers want to conduct a product that can prevent the diarrhea

cause by Escherichia coli (E.coli) and the main ingredient of this product is Blumea

Balsamifera. The researchers may prove it using Blumea Balsamifera tea but therefore

the researchers also expect that there’s a possibility to fail.

BACKGROUND OF THE STUDY

Based on the research the most affected of diarrhea is among children under five

years old in a country. Also marginalized people can encounter this kind of disease

because sometimes the food that they prepare for their daily meal is contaminated or

also known as “pagpag”. In this case the most prone to diarrhea are the families that are

marginalized so the researchers want to end those problems by using Blumea

balsamifera in killing Escherichia coli.

The researchers use Blumea balsamifera (sambong) in killing Escherichia coli

(E.coli). Sambong is an herbal plant that grows wild in tropical countries including the

Philippines. It has a long history in traditional medicine as a diuretic and analgesic.

Modern technology has made it possible to study sambong and its active ingredients.

Blumea balsamifera is used as an herbal treatment for the common cold and as a

diuretic in Philippine medicine, where it is most commonly known as sambong. It is used

to treat infected wounds, respiratory infections, and stomach pains in Thai

and Chinese folk medicine. Sambong is known for treating kidney stones, rheumatism,

coughs and colds, hypertension, heart disease and anti-diarrhea.

While Escherichia coli is a gram-negative, facultative anaerobic, rod shaped

bacterium of the genus Escherichia that is commonly found in the lower intestine of

warm-blooded organisms (endotherms). Most Escherichia coli strains are harmless, but

some serotypes can cause serious food poisoning in their hosts, and are occasionally

responsible for product recalls due to food contamination. The harmless strains are part

of the normal flora of the gut, and can benefit their hosts by producing vitamin K2, and

preventing colonization of the intestine pathogenic bacteria.

Diarrhea is abnormally frequent and watery bowel movements caused by E. coli

and the researchers want to prevent the said disease.

SCOPE AND LIMITATION

The research is about concentration that can kill microorganism. The researchers

want to kill Escherichia coli (E.coli) by using Blumea Balsamifera (sambong). The

researchers make this investigation to make a product with the main ingredient Blumea

balsamifera (sambong) that can cure diarrhea. The researchers bring their extract in

Natural Sciences Research Institute (NSRI) in University of the Philippines. The main

objective of the researchers in this investigation is to prove an alternative medicine

against the Escherichia coli (E.coli) in an affordable and easier way. Also the other

objective is to provide an herbal tea using Blumea Balsamifera (sambong). The

researchers get the result of the tested extract in Natural Sciences Research Institute

(NSRI) in University of the Philippines and if it is positive, they will test it in to the subject

(person) and observe what will happen after using it. The researchers do not use any

chemical substances in making the tea.

STATEMENT OF THE PROBLEM

The problem in this investigation is, the side effect of the product is not

constant and predictable. Also the researchers have not don’t know the specific

temperature to preserve the product. The researchers want to know if product can’t

affect the condition of the subject (person).

1. What is the effect of the Blumea balsamifera in treating diarrhea?

2. Is there any significant effect on the zone of inhibition in the concentration of

the Blumea balsamifera against Escherichia coli (E.coli)?

a. 100 %

b.75 %

c.50 %

3. How did the Blumea Balsamifera kill Escherichia coli (E.coli)?

HYPOTHESES

There is no significant effect on the gram-negative bacteria.

CHAPTER II:

EVALUATION, DESIGN AND FRAMEWORK

In this chapter, the resarchers will explain what product is all about. The

researchers will evaluate the process of making the herbal tea using Blumea

balsamifera (Sambong). The reseasrchers give an appropiate design of the product

want to conduct. Also the variables that the reserachers used. Using methodologies,

dependent, independent and other article the researchers may prove that this herbal

medicine is effectively to kill the said microorganisms.

Discussion of expected Output and Justification

This experiment is about the extracted Blumea balsamifera (Sambong) use a

main ingredient to make an herbal tea. The researchers conduct this output (herbal tea)

because tea can prevent diarrhea. It is affordable and easy to make. The researchers

want to prove that herbal tea with main ingredient Blumea balsamifera (Sambong) is

effective in killing Escherichia coli (E.coli). The researchers expect that the possible

result is perfect.

DEFINITION OF TERMS

Blumea balsamifera

- An herbal medicine usually grows in the Philippines in open grasslands, and a

widely used herbal treatment country. Known for remarkable treatment for kidney

stones, rheumatism, coughs and colds, hypertension, heart disease and anti-

diarrhea.

Diarrhea

- Abnormally frequent and watery bowel movements caused by E. coli

Escherichia coli (E.coli)

- Bacteria normally live in the intestines of healthy people and animals.

Marginalize

- Poor than a poor.

Agar Plate

- Is a Petri dish that contains a growth medium (typically agar plus nutrients) used

to culture microorganismsor small plants like the moss Physcomitrella patens.

Pathogenic bacteria

- Are bacteria that cause bacterial infection.

THEORITICAL FRAMEWORK

Based on the research, Blumea balsamifera is a source of the flavonoids

blumeatin, flavone and quercetin. Flavonoids or bioflavonoids are plant pigments which

act like antioxidants, protecting our cells and DNA from being damaged by free radicals.

Initial findings show that the blumeatin extracted from sambong leaves may protect our

liver from damage. Still other studies show that extracts from sambong tend to inhibit

the growth of cancer cells, whether the cells be from mice or people. Investigations

further opened up the possibility that the quercetin from sambong may be a strong

antioxidant and inflammatory, and have other anti-aging health benefits such as keeping

skin supple and looking young and healthy. Lastly, the actions of the antioxidant

flavones may prevent the development of cancer and heart disease. Sambong extracts

also display antifungal, antibacterial and antihistamine activities.

It is an astringent that can be used for the faster healing of cuts and wounds, for

post-partum baths and for bathing sensitive and painful areas affected by rheumatism. It

is an expectorant and sambong leaves can be prepared as a herbal tea that helps

relieve the symptoms of a cold by expelling mucus and phlegm from the respiratory

system.Like coffee and tea, sambong is a diuretic that facilitates the removal of excess

salt and water from our bodies and helps to dissolve kidney stones Findings from

clinical studies conducted by the Philippine Department of Health support the potential

of sambong as a safe and effective way to treat kidney stones. The Philippine National

Kidney and Transplant Institute has already approved the use of the herb for patients

with kidney problems in preventing or delaying dialysis and kidney transplants. Health.

CONCEPTUAL FRAMEWORK

I.P.O Paradigm

Input- In this study the researchers input is the Blumea balsamifera leaves. The

researchers choose this as main ingredient to test if it is effective.

Process– the researchers choose the first process by gathering materials it is because

the researcher cannot start without it. Then culturing the bacteria that will be use to test

Blumea balsamifera extract. Next the isolating the bacteria, doing the product (tea),

testing the product and then observe the result.

Output – After doing the process the researchers make a product that can cure the

diarrhea.

INP

UT

* Blumea balsamifera

leaves

PR

OC

ES

S

*Gathering materials

* Culturation

* Isolation

*Doing the product

*Testing the product

*Observation

*Result

OU

TP

UT

* Blumea balsamifera tea

The Variables, Their definitions and importance of the study

Independent variable:

Blumea balsamifera – Blumea balsamifera (sambong) is an herbal medicine usually

grows in the Philippines in open grassland, and a widely used herbal treatment in the

country. It is known for its remarkable treatment for anti-diarrhea and use from stomach

problems indigestion and diarrhea spasm.

Dependent variable:

Blumea balsamifera Tea- in our culture tea is very effective alternative to lessen the

stomach cramp, taken as tea, the leaves of sambong help control the diarrhea and

relieve stomach pains. Some people also use the plant as a drug for its hallugenic

effect. The researchers use this for its tested and recommended herbal medicine.

Controlled variable:

Chloramphenicol- is the controlled variable because it is has the standard of

significance. The researchers can say it is significance if i t passed the standard of the

measure of the zone of inhibition of the controlled variable.

Importance of the study:

Is to control the number of people who involve in this case which is diarrhea according

to study the most prone on it are the people who actively to eat an food which is

contaminated or some water that is not totally filtered neither raw .

CHAPTER 3: RESEARCH METHODOLOGY

The researchers tested their extract in Natural Science Research Institute (NSRI)

in University of the Philippines, to test the effectiveness of the Blumea balsamifera to

the Escherichia coli. The researchers use the leaves of Blumea balsamifera as the main

subject of the research and used 100 % concentration. Microbial suspension was

prepared from 24-hour old culture of the test organism. The suspending medium use

was 0.1% peptone water. Pre-poured Nutrient Agar (NA) plates, about 3 mm thick, were

inoculated with the microbial suspension by swabbing the agar surface. The cotton on

swab on an applicator stick was dipped into the microbial suspension, rotated several

time and pressed firmly on the inside wall of the tube above the fluid level to remove

excess inoculums from the swab. The swab was streaked over the entire agar surface.

This procedure was repeated in two more times, rotating the plate 60 degree each time

to ensure even distribution of the inoculums. Three equidistant wells were on the agar

plate using a cork bore (10mm diameter). Two hundred (200) µl of the sample was

placed in each well. For the positive control, a chloramphenicol disc was placed on the

center of the agar plate. The NA plates were incubated at 35ºC and observed after 24

hours. The clearing zone was measured in millimeters and the average diameter of the

clearing zones was calculated. The antimicrobial index (Al) was the following formula:

RESEARCH DESIGN

The researchers know that there are many ways to conduct this experiment

that’s why the researchers think the best way to introduce the product that the

researchers made. The researchers manipulate one variable, and control/randomizes

the rest of the variables the researchers used. The researchers has a gatherings thow

to done this investigatory project. The researchers assigned in different task or chapters

to done it to have an easy way to finish the experiment. If every researchers done with

their task it is the time they gather and start planing about the said investigatory project.

The researchers got some info in books and internet to have an acceptable reseacrh.

SETTING OF THE STUDY

This study was conducted at the researchers house where located in Block2 Lot8

Bistekville 1 brgy. Payatas B Quezon City. The researchers used this place because

they are comfortable and it’s because it has a big enough space, this place is also near

in some store where the researchers can find some of the ingredients that will be use in

the study, also in Kaimito st. Payatas B Quezon city where the researchers used this

place to make a documents about their studies.

The testing was held in Natural Science Research Institute (NSRI), where in

University of the Philippines, Diliman.

SUBJECT OF THE STUDY

Escherichia coli is a gram-negative, facultative anaerobic, rod shaped bacterium

of the genus Escherichia that is commonly found in the lower intestine of warm-blooded

organisms (endotherms). Most Escherichia coli strains are harmless, but some

serotypes can cause serious food poisoning in their hosts, and are occasionally

responsible for product recalls due to food contamination. The harmless strains are part

of the normal flora of the gut, and can benefit their hosts by producing vitamin K2, and

preventing colonization of the intestine pathogenic bacteria. The researchers want to kill

this kind of bacteria.

PROCEDURE OF THE STUDY

Materials:

Dried sambong leaves (1-2 days)

Tea Filter

Bowl

Teaspoon

Plastic bags

Procedure

First, gently crushed the dried sambong until it loosen and get a tea filter. Next

put 1-2 tsp.of loose leaves in tea filter, then seal with staple lastly packed in

appropriate plastic bag.

SOURCES OF DATA

The researchers tested their extract in Natural Science Research Institute (NSRI)

in University of the Philippines, to test the effectiveness of the Blumea balsamifera to

the Escherichia coli. Natural Science Research Institute (NSRI) is the one who tested

the extract and they use Agar Plate, Microbal suspension and Chorampinecol.

STATISTICAL TREATMENT

Frequency

Is the number of occurrences of a repeating event per unit time. It is also referred to

as temporal frequency, which emphasizes the contrast tospatial frequency and angular

frequency. The period is the duration of one cyclein a repeating event, so the period is

the reciprocal of the frequency.

Percentage

Is a number or ratio expressed as a fraction of 100. It is often denoted using

the percent sign, "%", or the abbreviation "pct."

CHAPTER 4: FINDINGS

Presentations Analysis and Interpretation of Data

Table 1

“The Extraction of Blumea

Balsamifera

Extract”

ml % rank

Squeezing 2.5 14.29 3

Decoction 5 28.57 2

Grinding 10 57.14 1

17.50 100

.

The graph shows about the result of diffirent steps in extraction of Blumea

balsamifera. The result is very clear that the researchers can get more extract if the

leaves of Blumea balsamifera grind, also the researchers consider te cleanliness of the

materials.

CHAPTER V

SUMMARY OF FINDINGS, CONCLUSION, RECOMMENDATION

Summary

According to the research of those researchers the Blumea balsamifera extracts

taken from Blumea balsamifera (sambong) leaves. According to the study about Blumea

balsamifera it can fight Escherichia coli when diarrhea attacks. Also the amount

concentration of the extract may affect the result of the effect against Escherichia coli.

Conclusion

Based on the result of the given experiment on Natural Sciences Research

Institute (NSRI) at University of the Philippines the Blumea balsamifera is a negative

gram bacterium in other term this result says that Blumea Balsamifera can’t prevent the

stomach cramps when diarrhea attacks. But the researchers think about that this leaves

can also fight against Escherichia coli because of some article that the researchers read

about it, maybe the tested Blumea balsamifera during examination is contaminated that

caused negative result but still the researcher aiming that they can do it before time of

submission still have faith in our God.

Recommendation

The researchers recommend that you can freely to drink this said tea to lessen

the stomach cramps when the diarrhea attacks because sambong is now registered and

approved by the Food and Drug Administration as a medicine. If the patient has

diarrhea you should maintain to drink him/her water.

BIBLIOGRAPHY

- http://www.webmd.com/digestive-disorders/features/diarrhea-common-triggers-

treatments

- http://www.wikihow.com/Cure-Diarrhea

- http://health.howstuffworks.com/wellness/natural-medicine/home-remedies/how-to-

cure-diarrhea.htm

- http://www.ncbi.nlm.nih.gov/books/NBK11764/

- http://www.medicalhealthguide.com/articles/sambong.htm

- http://en.wikipedia.org/wiki/Percentage

- en.wikipedia.org/wiki/Frequency

- http://neuleaftea.com/sambong/nutrients-benefits.php

- http://www.herbaldb.com/sambong-for-diabetes-is-it-really-effective/

DIAZ, XENA NICOLE P.

Kaimito st. Payatas B Quezon City

AWARDS

2nd Honor, Best in Values

TRAININGS:

NONE

PERSONAL INFORMATION

AGE: 15

GENDER: Female

DATE OF BIRTH: June 30 1999

PLACE OF BIRTH: Querino Memorial Center

CIVIL STATUS: Single

CITIZENSHIP: Filipino

MOTHER’S NAME: Rebecca P. Diaz

OCCUPATION: None

FATHER: Edwin R. Diaz

OCCUPATION: Mechanic

RELIGION: Christian Born Again

EDUCATIONAL BACKGROUND

SESONDARY EDUCATION

Justice Cecilia Munoz Palma High School

Payatas B Quezon City

PRIMARY EDUCATION

Melencio M. Castelo Elementary School Payatas B. Q.C

LLIDO, DANICE Y.

Lower Atis St. Payatas B Quezon City

AWARDS:

NONE

TRAININGS:

NONE

PERSONAL INFORMATION

AGE: 14

GENDER: Female

DATE OF BIRTH: June 30 2000

PLACE OF BIRTH: Mandaluyong City

CIVIL STATUS: Single

CITIZENSHIP: Filipino

MOTHER’S NAME: Marivic Y. Llido

OCCUPATION: House Wife

FATHER: Daniel C. Llido

OCCUPATION: Trailer truck Driver

RELIGION: Christian Born Again

EDUCATIONAL BACKGROUND

SESONDARY EDUCATION

Justice Cecilia Munoz Palma High School

Payatas B Quezon City

PRIMARY EDUCATION

Melencio M. Castelo Elementary School Payatas B. Q.C

FEDELIS, JAY PAUL

14 Phase 2 purok 8 payatas B. Quezon City

AWARDS:

MATHEMAGICIAN CHAMPION

TRAININGS:

NONE

PERSONAL INFORMATION

AGE: 15

GENDER: male

DATE OF BIRTH: July 10 1999

PLACE OF BIRTH: Muntinlupa City

CIVIL STATUS: Single

CITIZENSHIP: Filipino

MOTHER’S NAME: Paulita Fedelis

OCCUPATION: House wife

FATHER: Romulo Fedelis

OCCUPATION: Traffic enforcer

RELIGION: Christian Born Again

EDUCATIONAL BACKGROUND

SESONDARY EDUCATION

Justice Cecilia Munoz Palma High School

Payatas B Quezon City

PRIMARY EDUCATION

Lupang Pangako Elementary School

DEL CARMEN, DENICE LEA

Block 2 Lot 8 Bistekville 1 brgy. Payatas B Quezon City

AWARDS:

1st Honor

TRAININGS:

NONE

PERSONAL INFORMATION

AGE: 15

GENDER: Female

DATE OF BIRTH: February 12 2000

PLACE OF BIRTH: Quezon City

CIVIL STATUS: Single

CITIZENSHIP: Filipino

MOTHER’S NAME: Ma. Gemma Del Camen

OCCUPATION: Teacher

FATHER: Marlon Del Carmen

OCCUPATION: Driver

RELIGION: Christian Born Again

EDUCATIONAL BACKGROUND

SESONDARY EDUCATION

Justice Cecilia Munoz Palma High School

Payatas B Quezon City

PRIMARY EDUCATION

Benigno Aquino Jr. Elementary School

ESPINOSA, MARIELEN

AWARDS:

TRAININGS:

NONE

PERSONAL INFORMATION

AGE: 15

GENDER: Female

DATE OF BIRTH:

PLACE OF BIRTH:

CIVIL STATUS: Single

CITIZENSHIP: Filipino

MOTHER’S NAME:

OCCUPATION:

FATHER:

OCCUPATION:

RELIGION: Christian Born Again

EDUCATIONAL BACKGROUND

SESONDARY EDUCATION

Justice Cecilia Munoz Palma High School

Payatas B Quezon City

PRIMARY EDUCATION

CANTUANGCO, JESRICK

AWARDS:

1ST Honor

TRAININGS:

NONE

PERSONAL INFORMATION

AGE: 15

GENDER: Male

DATE OF BIRTH:

PLACE OF BIRTH:

CIVIL STATUS: Single

CITIZENSHIP: Filipino

MOTHER’S NAME:

OCCUPATION:

FATHER:

OCCUPATION:

RELIGION: Christian Born Again

EDUCATIONAL BACKGROUND

SESONDARY EDUCATION

Justice Cecilia Munoz Palma High School

Payatas B Quezon City

PRIMARY EDUCATION

ORDOVEZA, GENEVA MAE

14 Makisig St. Spiritual cmpd.

AWARDS:

5th place at Pagsulat ng lathalain

TRAININGS:

Journalism

PERSONAL INFORMATION

AGE: 15

GENDER: Female

DATE OF BIRTH: February 12 2000

PLACE OF BIRTH: Sta. Monica Hospital

CIVIL STATUS: Single

CITIZENSHIP: Filipino

MOTHER’S NAME: Jojie D. Ordoveza

OCCUPATION: House Wife

FATHER: Juancho V. Ordoveza

OCCUPATION: Safety Officer

RELIGION: Christian Catholic

EDUCATIONAL BACKGROUND

SESONDARY EDUCAT

Justice Cecilia Munoz Palma High School

Payatas B Quezon City

PRIMARY EDUCATION

Payatas B. Elementary School

APPROVAL SHEET

Dear Sir Denis Dyvee Errabo,

Good day sir! We did this letter to ask permission for our investigatory project

because we know that approval of the teacher is important before we start doing this

project.

Our project is all about the concentration of sambong that can kill the bacteria

such as Escherichia coli (E.coli). Nowadays, many people suffering from many diseases

like hyper tension, kidney cancer, stomach ailments etc so that we choose sambong to

make a tea as our main product to fight the said diseases.

Based on the study and research of the researchers, sambong tea has

flavonoids which are from a plant and act as anti-oxidant that can makes our human

body cells and DNA totally free from radicals.

We will continue to study more about our product. We hope for your approval and

understanding about this matter. Thank You!

Sincerely yours,

The Researchers