Sample Pretreatment, Accessories · High-molecular weight compounds (proteins, DNA, sugars, etc.)...

YMC GENERAL CATALOG

9Sample Pretreatment, Accessories

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Sample pretreatment

Column protection

Tubing parts

Empty columns and other parts

Pressure resistant empty glass columns

165

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YMC Dispo SPE C18

Product No. Specification

XQSPEC18101 C18 100mg/1mL 100-packXQSPEC18203 C18 200mg/3mL 50-packXQSPEC18503 C18 500mg/3mL 50-packXQSPEC18A06 C18 1000mg/6mL 30-pack

YMC Dispo SPE SIL

Product No. Specification

XQSPESIL503 SIL 500mg/3mL 50-packXQSPESILA06 SIL 1000mg/6mL 30-pack

Sample pretreatment, Accessories

Material properties of productsS t a i n l e s s（SUS-316）

A general-purpose material for general HPLC products.Contains Ni, Cr, Mo, etc, as well as Fe, and is highly resistant to corrosion.

P E E K（polyetheretherketone）

A super engineering plastic developed as an alternative to metals.A polymer containing ketones and ether bonds, it is highly resistant to pressure, chemicals, heat, and fatigue. Used as an alternative to stainless for a metal-free tubing system.

T e f l o n（polytetrafluoroethylene, PTFE）

A polymer of tetrafluoroethylene. Highly resistant to chemicals but not so resistant to pressure as PEEK. Generally used for low pressure tubing systems.(Teflon is a trademark of DuPont.)

P o l y a c e t a lAn engineering plastic. A polymer in which aldehyde compounds are polymerized, with excellent chemical resistance and excellent machinability.

P o l y p r o p y l e n eA polymer of propylene. Excellent chemical and heat resistance. Often used for containers since processing, such as injection molding, is easy.

Sample pretreatment1Sample pretreatment cartridge YMC Dispo SPE●Disposable type cartridge made from polypropylene● Utilizes packing material designed for HPLC columns,

manufactured under strict quality controls●Excellent cartridge-to-cartridge reproducibility●High recovery of samples

Excellent recovery

Sample YMC Dispo SPE

Cartridge,Company A

Cartridge,Company B

Basic sample (Propranolol hydrochloride) 89（99）％ 59（73）％ 66（89）％Acidic sample (Thioctic acid) 91（100）％ 92（89）％ 87（88）％＊Figures inside parentheses represent recoveries obtained when buffer was used.

Vacuum

Syringe

Vacuum method Compression method Centrifugation

YMC Dispo SPE

YMC D

ispo SPE

Adapter

YMC Dispo SPE

●Vacuum method is suitable for large-scale treatment and compression method is suitable for small-scale treatment

Method of use

ContainerMade from medical-grade polypropylenewith minimal elution of additives.

Upper and lower fritsPore size of 20 µm

Luer tipCan be connected to commerciallyavailable vacuum solid phaseextraction systems.

Solid phaseYMC＊GEL

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Column protection2

YMC guard cartridges YMC-Pack

Precolumn filter

Stainless steel precolumn filters are convenient for the case shown below:

One of the causes of a gradual increase of a column pressure after each sample injection is that fine particles in the sample clog the frit of the column. Replacing the column frit is one solution; however it is more convenient to use precolumn filter. Connect a precolumn filter between the injector and the column, check the line pressure occasionally, and replace the precolumn filter when the line pressure increases.Stainless steel precolumn filters are suitable for stainless steel tubing systems. Both PEEK and stainless steel precolumn filters are suitable for PEEK tubing systems.

Problem prevention

High-molecular weight compounds (proteins, DNA, sugars, etc.) that easily and irreversibly adsorb to the main column are likely to cause a pressure increase and separation failure. When these compounds adsorb to the main column, it is difficult to recover column performance by cleaning.A guard column will protect the main column when fouling of the main column is due to contamination from sample matrices.

Stainless steel precolumn filter●Filter construction with minimal dead volume

Effective diameter of the filter is extremely small about 1.6 mm. Sample diffusion that occurs in passage is minimized.

Stainless steel precolumn filterProduct No. SpecificationXRPRCS02 For tubing system with 1/16” outer diameter, 1 filter includedXRPRCS25 5 spare filters 2 µm are included

RetainerFilter

PEEK precolumn filter●Metal free

The material of the wetted part of the holder is PEEK and the packing is PCTFE. The packaging that of the filter is a mixture of PEEK and Teflon. PEEK precolumn filters are suitable for use in HPLC systems requiring a metal-free environment.

●High-pressure resistancePEEK precolumn filters are resistant to ca. 35 MPa.

PEEK precolumn filterProduct No. SpecificationXRPRCP02 For tubing systems with 1/16” outer diameter, 1 filter includedXRPRCP25 5 spare filters 2 µm are included

Filter

1 23

4

10 20 min0

Main column only

Theoretical plate number（peak 4）：Pressure（MPa）：

11,200±200（n=3）5.5±0（n=3）

12

34

10 20 min0

After connection ofa precolumn filter

Theoretical plate number（peak 4）：Pressure（MPa）：

11,500±300（n=3）5.5±0（n=3）

Column ： YMC-Pack ODS-A (5 µm, 120 Å) 150×4.6 mmI.D.

Eluent ：acetonitrile/water (60/40)Flow rate ：1.0 mL/minTemperature ：37 ℃Detection ：UV at 270 nm, 0.32 AUFSSample ： 1. Uracil (0.05 mg/mL)

2. Methyl benzoate (0.5 µL/mL) 3. Butyl benzoate (1.5 µL/mL) 4. Butylbenzene (12.0 µL/mL)

There is no decrease of column efficiency due to connection of a precolumn filter.

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YMC guard cartridges●Wide variety of packing materials

YMC＊GEL with various functional groups identical to those available for analytical columns is packed under high pressure.

●DisposableThe cartridge holder can be used repeatedly. When the YMC guard cartridge needs replacement must be replaced. Only the cartridge column.

●Easy cartridge replacementThe main body of the YMC guard cartridge is composed of a cartridge holder and a cartridge column. The cartridge column is threaded for easy replacement.

cartridge column

cartridge holder

YMC guard cartridgesGuard cartridge holders

Product No. SpecificationXPCHW For a cartridge column

Cartridge columns（3-pack）Product No. Specification

AS12S05-G304CC For YMC-Pack Pro C18AA12S05-G304CC For YMC-Pack ODS-AAM12S05-G304CC For YMC-Pack ODS-AMAQ12S05-G304CC For YMC-Pack ODS-AQ

●Suitable for analytical columns with 4.0 to 6.0 mm inner diameters.！△For other functional groups, see pages for each analytical column.A guard cartridge holder will need to be purchased separately before using this product for the first time.

12

3

5 10 min0

Main column only

12

3

5 10 min0

After connection ofYMC guard cartridge

12

3

5 10 min0

After connection of CompanyA’s guard cartridge

This figure shows chromatograms produced using a YMC-Pack ODS-AM(150×4.6 mm I.D.) not-connected to a guard cartridge (Left), while connected a YMC guard cartridge (Center), and while connected to Company A’s guard cartridge (Right).

Semi-micro guard cartridgesSemi-micro guard cartridge holder（commonly used for inner diameters of 1.0, 1.5, and 2.0 mm）

Product No. SpecificationXPCHSMW For a semi-micro cartridge column

Semi-micro guard cartridge for columns with 2.0 mm inner diameter（2-pack）Product No. Specification

AS12S05-0102CC For YMC-Pack Pro C18AA12S05-0102CC For YMC-Pack ODS-AAM12S05-0102CC For YMC-Pack ODS-AMAQ12S05-0102CC For YMC-Pack ODS-AQ

●Suitable for analytical columns with inner diameters of 2.0 to 3.0 mm.！△For columns with inner diameters of 1.0 mm and 1.5 mm, see pages for each analytical column.A semi-micro guard cartridge holder will need to be purchased separately before using this product for the first time.

Column protection2

Eluent ： 20 mM K2HPO4-KH2PO4 (pH 7.6) /methanol (40/60)

Flow rate ：1.0 mL/minTemperature ：37 ℃Detection ：UV at 254 nm, 0.32 AUFSInjection ：5 µL (70 ～ 250 µg/mL)（When a different packing material from that of the main column is used, peak shapes may be affected.）

N

OH

O O

NH

O

O

HN1. Uracil

2. Acetylacetone

3. 8-Hydroxy quinoline


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Universal connector●Enables perfect column connectivity

A movable ferrule and a fixing screw enable secure connection to the column endfitting. There is no cavity at the top of the ferrule.

●Super high-pressure resistanceUniversal connectors are high performance connectors resistant to high pressure up to 70 MPa when tightened only by hand.

●For connection to a guard columnThe double type is the most suitable for connecting of a main column and a guard column.

Double type

Single type

Universal connector（single）Product No. SpecificationXRSFS520 1/16” outer diameter,0.005” inner diameter, 20cm lengthXRSFS220 1/16” outer diameter,0.020” inner diameter, 20cm length

Universal connector（double）Product No. SpecificationXRSFD506 1/16” outer diameter,0.005” inner diameter, 6cm lengthXRSFD510 1/16” outer diameter,0.005” inner diameter, 10cm lengthXRSFD520 1/16” outer diameter,0.005” inner diameter, 20cm lengthXRSFD220 1/16” outer diameter,0.020” inner diameter, 20cm length

Universal connector（Single flexible）Product No. SpecificationXRSFS528F 1/32” outer diameter,0.005” inner diameter, 28cm lengthXRSFS540F 1/32” outer diameter,0.005” inner diameter, 40cm length

●outer diamater of metted part is 1/16"

Spare ferrule for universal connectorsProduct No. Specification

XRSFV Vespel sealing ferrule, 5-pack

●Tightening the fixing screw forces a tube to squeeze into the column as far as it will go, and the tube makes tight contact with the screw hole.Everyone can connect columns easily and securely.

The tube makes tight contactwith the screw hole bytightening a fixing screw.

No need for span

ners

　　easy and reli

able.Fixing screw

Fixing ferrule

Sealing ferrule（replacable）

Adjuster screw

Tubing parts3

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Handy connector 1●A ferrule integrated into a connector

Handy connector 1 resists pressure to ca. 21 MPa. Handy connector 1 is suitable for tubing systems of relatively low pressure. Connection to a tubing system is easy because a ferrule is integrated into the connector.

●Metal freeThe whole connector is made from PEEK. Handy connector 1 is suitable for metal-free tubing systems using PEEK tubes.

Handy connector 1Product No. Specification

XRP0203 For tubing systems of 1/16” outer diameter, 10-pack

PEEK

Tubing parts3

Handy connector 2●High pressure resistance and made from stainless steel

Handy connector 2 resists pressure to ca. 42 MPa. Handy connector 2 is suitable for connection to the injection side of a column, to which high pressure is applied. It is also useful for connection of a guard column or a precolumn filter.

●Metal freeThe ferrule at the wetted part of the connector is made from PEEK. Handy connector 2 is suitable for metal-free tubing systems using PEEK tubes.

●Ferrule is replaceableOnly the ferrule needs to be replaced when it deteriorates, as opposed to the entire connector.

Handy connector 2Product No. Specification

XRP0204 For tubing system of 1/16” outer diameter, 2-packXRP0205 Spare PEEK ferrule, 10-pack

● Be aware that Handy connector 2 cannot be connected to “Old Shimadzu type”, “Old JASCO type”, or “Old Hitachi type” tubes.

Stainless steel Ferrule for replacement

No need for spann

ers

　　easy and relia

ble. （3）Turn the connector while pushing the tube to connect the tube and connector.

（4）Be sure that there is no leak from the screw hole.

（1）Extend the top of PEEK tube beyond the ferrule.

（2）Force the tube to make tight contact with the bottom of the screw hole.


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Outer diameter1/16”

Inner diameter0.127mm

0.25mm 0.5mm 0.8mm

Female nut for 1/8″ tubing system （cap nut）（stainless steel）

Ferrule for 1/8″ tubing system（stainless steel, double ring）

Outer diameter1/16” Outer diameter1/8”

Inner diameter0.25mm

0.5mm 0.8mm 2.0mm

Female nut for 1/8″tubing system

Ferrule for 1/8″ tubing system

Male nut for 1/16″tubing system

Ferrule for 1/16″ tubing system

Stainless steel PEEK

Cross section

Union for 1/16″ tubing systemUnions for 1/16” tubing system come in two types, made from either stainless steel or PEEK. They are convenient to use it with a Handy connector. Connector type is “W type”.

Column couplerColumn couplers are used for column-to-column connections. Various tube inner diameters and column connection types are available.

PEEK tubing●Easy to use

PEEK tubing is made from a material that is flexible and easy to use. It is easily cut using a commercially available cutting knife. A PEEK tube with an inner diameter of 0.127 mm is useable at up to 40 MPa. PEEK tubes are practical general tubing as alternatives to stainless steel tubing. They are suitable for metal-free tubing systems. A Handy connector is suitable for connection of PEEK tubing to a column.

Stainless steel tubing●Stainless steel male nut and ferrule are used for connection

Tube cutter●For cutting 1/16” stainless tubing （simple type）

Male nut for 1/16″ tubing system （stainless steel）

W type P type Old Shimadzu type Old Hitachi typeOld JASCO type

Ferrule for 1/16″ tubing system （stainless steel）

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Tubing parts3

Union for 1/16” tubing system

Column coupler

（page 138）

YMC-Pack

guard cartridges

PEEK tubingProduct No. Specification

XRPT01 1/16” outer diameter, 0.127 mm inner diameter, 3m lengthXRPT02 1/16” outer diameter, 0.25 mm inner diameter, 3m lengthXRPT05 1/16” outer diameter, 0.5 mm inner diameter, 3m lengthXRPT08 1/16” outer diameter, 0.8 mm inner diameter, 3m length

Stainless steel tubingProduct No. Specification

XRST02 1/16” outer diameter, 0.25 mm inner diameter, 2m lengthXRST05 1/16” outer diameter, 0.5 mm inner diameter, 2m lengthXRST08 1/16” outer diameter, 0.8 mm inner diameter, 2m lengthXRST20 1/8” outer diameter, 2.0 mm inner diameter, 5m length

Tube cutterProduct No. Specification

XRP1909 For cutting 1/16” stainless tubing

Male nut for 1/16” tubing system（stainless steel）Product No. Specification

XRONW 1/16” W type, P type, 5-packXRONS 1/16” Old Shimadzu type, 5-packXRONJ 1/16” Old JASCO type, 5-packXRONH 1/16” Old Hitachi type, 5-pack

Ferrule for 1/16” tubing system（stainless steel）Product No. Specification

XRONFA 1/16” one ring ferrule, 5-pack

Female nut for 1/8” tubing system（cap nut）（stainless steel）Product No. Specification

XRON8 1/8” female nut, 5-pack

Ferrule for 1/8” tubing system（stainless steel）Product No. Specification

XRTWFA Front ferrule, back ferrule, 5-pack each

Union for 1/16” tubing systemProduct No. Specification

XRUNW 1/16” tubing system （stainless steel）XRUN16P 1/16” tubing system （PEEK）

Column couplerProduct No. SpecificationXRCP1602 1/16” outer diameter, 0.25 mm inner diameterXRCP1605 1/16” outer diameter, 0.5 mm inner diameterXRCP1620 1/16” outer diameter, 0.8 mm inner diameter

（Stainless tube 50 mm×1, male nut×2, ferrule×2）

Notes for successful tubing

The shapes of separation peaks are affected not only by column performance, but by the tubing system as well.Some effort is needed to minimize the dead volume in a tubing system among the injector column and detector, in order to prevent poor peak shape. Using a tube with a small inner diameter (0.127 mm) is one solution. When a tube is connected to a union or a column, it is important to give due consideration to make the top of the tube contact flush with the bottom of the screw hole and to avoid leakage or creation of a cavity.


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Empty columns and other parts4

Analytical column

Semi-preparative column

Inner diameter 2.0～10mm

Inner diameter 20～30mm Inner diameter

50～200mmInner diameter 4.0～6.0mm

1 YMC original empty columns 2 Ferrule type empty column 3 Flange type empty column

YMC original empty columns（for analysis or semi-preparative isolation）A stainless steel tube with a screw and an endfitting are directly connected (screw type). Since YMC original empty columns use no ferrule, there is no danger of endfitting dislocation when the column is packed and used under high pressure.

YMC original empty columns

Product No. Column sizelength×inner diameter（mm）

XC0502WT 502.0XC1502WT 150

XC2502WT 250XC0346WT 30

4.6

XC0546WT 50XC1046WT 100XC1546WT 150XC2546WT 250XC3046WT 300XC0506WT 50

6.0

XC1006WT 100XC1506WT 150XC2506WT 250XC3006WT 300XC5006WT 500XC0308WT 30

8.0XC3008WT 300XC5008WT 500XC0310WT 30

10.0XC1510WT 150XC2510WT 250XC3010WT 300XC5010WT 500


XC0520WT 50

20.0

XC1520WT 150XC2520WT 250XC3020WT 300XC5020WT 500XCA020WT 1000XCL530WT 75

30.0XC2530WT 250XC3030WT 300XC5030WT 500XCA030WT 1000

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Column end assembly

Frit

Frit

Spacer

Distributor

Column end assembly

YMC original empty column partsProduct No. Product name

XPEF02 For 2.0 mm inner diameter, Column end assembly, 1-packXPEF46 For 4.6 mm inner diameter, Column end assembly, 1-packXPEF06 For 6.0 mm inner diameter, Column end assembly, 1-packXPEF08 For 8.0 mm inner diameter, Column end assembly, 1-packXPEF10 For 10 mm inner diameter, Column end assembly, 1-packXPEF20 For 20 mm inner diameter, Column end assembly, 1-packXPEF30 For 30 mm inner diameter, Column end assembly, 1-packXPSF02S For 2.0 mm inner diameter, Frit, 5-packXPSF46S For 4.6 mm inner diameter, Frit, 5-packXPSF06S For 6.0 mm inner diameter, Frit, 5-packXPSF08S For 8.0 mm inner diameter, Frit, 5-packXPSF10S For 10 mm. inner diameter, Frit, 5-packXPSF20S For 20 mm. inner diameter, Frit, 2-packXPSF30S For 30 mm. inner diameter, Frit, 2-packXPSS20S For 20 mm. inner diameter, Spacer, 1-packXPSS30S For 30 mm. inner diameter, Spacer, 1-pack

●Frits with 20 mm or more inner diameter include distributors.！△Connector type is “W type”.

Main column part names

FritA disk type part used to keep the packing material in a column. A frit with a pore size of 2 µm is generally used for 5 µm packing material.

DistributorA part used to uniformly introduce a sample in mobile phase to a column. Its structure enables uniform diffusion of the sample over the frit surface.

Inner diameter 2.0mm、4.6mm、6mm、8mm、10mm

Inner diameter 20mm、30mm


YMC original empty column parts

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Ferrule type empty columnFerrule type empty columns are standard-type empty columns. A stainless steel tube and an endfitting are tightly connected using a ferrule.

Ferrule type empty column


XC0104WF 10 4.0XC0105WF 10 5.0XC0546WF 50

4.6XC1046WF 100XC1546WF 150XC2546WF 250

Ferrule type empty column partsProduct No. Product name

XPEF46F For 4.6 mm inner diameter, Column end assembly, 1-packXPEF04F For 4.0 mm inner diameter, Column end assembly, 1-packXPEF05F For 5.0 mm inner diameter, Column end assembly, 1-packXPSF46F For 4.6 mm inner diameter, Frit, 5-packXPSF04F For 4.0 mm inner diameter, Frit, 5-packXPSF05F For 5.0 mm inner diameter, Frit, 5-pack

Column end assembly

Frit

Flange type empty column （for preparative isolation）A stainless steel tube with a flange and a cap are tightly connected using a bolt. The inner diameter is 50 mm or more.

Flange type empty column50mm inner diameter


Max pressure（MPa）

XC0551■R 50

50.0 7

XC1051■R 100XC2551■R 250XC3051■R 300XC5051■R 500XCA051■R 1000XC0552■R 50

50.0 14

XC1052■R 100XC2552■R 250XC3052■R 300XC5052■R 500XCA052■R 1000

！△ Designate a frit pore size depending on particle size. We recommend 2 µm for particle size below 10 µm,

5 µm for particle size above 15 µm.

70mm inner diameter



XC1071■R 100

70.0 7XC2571■R 250XC3071■R 300XC5071■R 500XCA071■R 1000XC1072■R 100

70.0 14XC2572■R 250XC3072■R 300XC5072■R 500XCA072■R 1000

200mm inner diameter



XC20C1■R 200200.0 7XC50C1■R 500

XCA0C1■R 1000XC20C2■R 200

200.0 14XC50C2■R 500XCA0C2■R 1000

※ ■＝Connection type: Please select your desired connection type.

　 A：1/16" male nut　 D：1/8" female nut　 E：1/4" female nut




XC20B1■R 200

150.0 7XC30B1■R 300XC50B1■R 500XCA0B1■R 1000XC20B2■R 200

150.0 14XC30B2■R 300XC50B2■R 500XCA0B2■R 1000

！△ Designate a frit pore size depending on particle size. We recommend 2 µm for particle size below 10 µm,

5 µm for particle size above 15 µm.




XC10A1■R 100

100.0 7XC30A1■R 300XC50A1■R 500XCA0A1■R 1000XC10A2■R 100

100.0 14XC30A2■R 300XC50A2■R 500XCA0A2■R 1000

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Connection adaptor

Cap

Frit

Distributor

Spacer

Bolt set

Specification of flange type empty columnFlange type empty column parts

Product No. Product name Max pressure（MPa）

XPCP052 For inner diameter 50 mm, Cap 7XPCP053 For inner diameter 50 mm, Cap 14XPSF0522 For inner diameter 50 mm, 2 µm frit （with distributor） 7XPSF0532 For inner diameter 50 mm, 2 µm frit （with distributor） 14XPSF0525 For inner diameter 50 mm, 5 µm frit （with distributor） 7XPSF0535 For inner diameter 50 mm, 5 µm frit （with distributor） 14XPRI052 For inner diameter 50 mm, Stainless SS ring 7XPRI053 For inner diameter 50 mm, Stainless SS ring 14XPBL052 For inner diameter 50 mm, bolt set （8 pairs） 7XPBL053 For inner diameter 50 mm, bolt set （12 pairs） 14XPSU162 For inner diameter 50 mm, 1/16” male nut for connection adaptor 7XPSU163 For inner diameter 50 mm, 1/16” male nut for connection adaptor 14XPSU82 For inner diameter 50 mm, 1/08” female nut for connection adaptor 7XPSU83 For inner diameter 50 mm, 1/08” female nut for connection adaptor 14

＊For column parts with an inner diameter of 70 mm or more, please contact us.

Airtight stopperAirtight stoppers are used to prevent column contents from drying.Please use a stopper appropriate for each column connection type.

YMC original type W type 1/8" airtight stopper

1/16" airtight stopperProduct No. Specification

XPMSY YMC original type （PEEK）, 5-packXPMSW Waters type （stainless）, 5-pack

1/8" airtight stopperProduct No. Specification

XPMS8 Female nut, 2-pack


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●Suitable for preparative chromatographyPressure resistant empty glass columns are useful for early purification of natural products, after-treatment of open column chromatography and pre-treatment of preparative HPLC. They are also suitable for removal of impure compounds produced during organic synthesis.

●The most suitable for self-packingLow-cost preparative isolation can be achieved by self-packing. Pressure resistant empty glass columns are highly flexible tools for preparative isolation because many types of packing materials may be packed into the column.

●Pressure resistanceYMC empty glass columns, made from Pyrex glass, have excellent pressure resistance. They are suitable for spherical and irregular packing materials with a particle size of 50 µm or more.

● Applicable to medium-pressure and high-performance liquid chromatography systemsPressure resistant empty glass columns can be connected to existing chromatographs, making the preparative isolation easy.

●Metal free columnNo metal is used for wetted parts. There is no danger of corrosion caused by acids or of metal contamination in isolation fractions.Pressure resistant empty glass columns are suitable for a metal-free HPLC system.

●VisibilityIn contrast to stainless steel columns, the separation behavior of samples analyzed by chromatography can be observed visually. It is useful for preparative isolation or removal of colored material.

Pressure resistant empty glass columns5

Packing ：YMC＊GEL SIL, S-50 µm, 120 ÅColumn Size ：500×20 mmI.D.Eluent ：n-hexane/ethanol (90/10)Detection ：UV at 254 nm, 0.16 AUFSSample ：1. Toluene　2. NitrobenzeneInjection ：20 µL

0 5 10 15 min

INJ.

N=2,700

A

1

2

0 5 10 min

INJ.

N=2,200

B2

1

Dry-packed with S-50 gel （silica）

Flow RatePressure

10 mL/min0.2 MPa

20 mL/min0.3 MPa

These figures show typical measurement results for theoretical plate numbers obtained using two YMC pressure resistant glass columns, XC5020FG; one was dry-packed with C18 and the other with silica gel (S-50 gel, spherical type, particle size of 50 µm).For this column size, the volume of packing material used per column is ca. 80 g.

0 10 20 30 40 min

INJ.

1

2

3

4

N=2,500

Dry-packed with S-50 gel （ODS）

Packing ：YMC＊GEL ODS-A, S-50 µm, 120 ÅColumn Size ：500×20 mmI.D.Eluent ：methanol/water (75/25)Flow Rate ：10 mL/minPressure ：0.3 MPaDetection ：UV at 254 nm, 0.32 AUFSSample ：1. Uracil　2. Methyl benzoate　3. Toluene　4. NaphthaleneInjection ：20 µL

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Pressure resistant empty glass columns（glass tube, column endfitting, tubing fitting kit, airtight stopper）

Inner diameterProduct No.

Length100mm 300mm 500mm

8mm XC1008FG XC3008FG XC5008FG20mm XC1020FG XC3020FG XC5020FG30mm XC1030FG XC3030FG XC5030FG

Pressure resistant empty glass columns5

Parts①Glass tube

Inner diameterProduct No.

Length100mm 300mm 500mm

8mm XT1008FG XT3008FG XT5008FG20mm XT1020FG XT3020FG XT5020FG30mm XT1030FG XT3030FG XT5030FG

②Column endfitting （includes frit and packing）

Product No. SpecificationXPEA8 For inner diameter 8 mm, 1-packXPEA20 For inner diameter 20 mm, 1-packXPEA30 For inner diameter 30 mm, 1-pack

One column requires 2 column endfittings.

④Tubing fitting kit （ferrule, o-ring, male nut）

Product No. SpecificationXRFK001 For inner diameter 8, 20,30 mm （common use）

Fitting kit, a set of 2 kits, IN-side and OUT-side, for 1/16” O.D. Teflon tube or stainless tube.

③Glass frit （with packing）

Product No. SpecificationXPFP83 For inner diameter 8 mm, 3 µm pore sizeXPFP2010 For inner diameter 20 mm, 10 µm pore sizeXPFP3010 For inner diameter 30 mm, 10 µm pore size

⑤Special-purpose Teflon tubeProduct No. Specification

XRTT102 1/16” outer diameter, 0.25 mm inner diameter, 10 mXRTT105 1/16” outer diameter, 0.5 mm inner diameter, 10 m

For tubing fitting kits

Column connection joint （column union and flat seal（2-pack））

①Glass tubeProduct No. Specification

XRCJ8 For inner diameter 8 mmXRCJ20 For inner diameter 20 mmXRCJ30 For inner diameter 30 mm

Used for connection of columns with same diameter in packing.

1

3

2

4

5


For large columns not described above, please contact us.

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Sample Pretreatment, Accessories · High-molecular weight compounds (proteins, DNA, sugars, etc.)...

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