Salk Institute Mobile Lab Gel Electrophoresis Student Instructions VWR Set up.
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Transcript of Salk Institute Mobile Lab Gel Electrophoresis Student Instructions VWR Set up.
Salk Institute Mobile Lab
Gel Electrophoresis
Student Instructions
VWR
Set up
Salk Institute Mobile Lab
Gel Electrophoresis Student Station Set-up
tip container w/tips
waste container
Micro-pipettor
practice sheet in protector
foam epi rack w/ 1 food color tube each: orange, green, purple
Salk Institute Mobile Lab
How to Use a micro-pipettor
• Parts of a Micro-pipettor– Make sure a yellow tip is
firmly twisted onto the end of the micro-pipettor
– Adjust the dispense amount to 5 µl (05.0) by turning the knob at the top
Plunger
Amount window
Eject button
Barrel
Yellow tip
Salk Institute Mobile Lab
How to Use a micro-pipettor
• Carefully open one of the food color tubes – Don’t let the lid snap back
and splash you!
• To Fill – press down gently with your thumb until you feel the plunger stop. This is STOP 1. While holding the plunger at Stop 1 place the tip into the liquid and release the plunger. This will draw up the precise volume you want.
Salk Institute Mobile Lab
How to Use a micro-pipettor
• To Dispense – press down hard on the plunger, you’ll pass Stop 1 and continue to STOP 2. This will fully eject the volume from the tip.
• Using your other hand to guide the end of the tip, practice dispensing the 5µl drops – Try to not push down on the
plastic sheet - see if you can hold the pipettor tip just above the paper
Salk Institute Mobile Lab
How to Use a micro-pipettor
• Push green button to eject the dirty tip into the waste container and replace with a clean tip
• Always use a clean tip each time!!
Salk Institute Mobile Lab
How to Use a micro-pipettor
• Ready to load a real gel?
Salk Institute Mobile Lab
Loading the gel
• Load the food color solutions into the wells on your group’s gel
• Be careful not to poke through the bottom of the wells!