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S E A R C H & D I S C O V E R Y - isu.edu.t · Reconstruction」,本書是全世界第一本以...
Transcript of S E A R C H & D I S C O V E R Y - isu.edu.t · Reconstruction」,本書是全世界第一本以...
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S E A R C H & D I S C O V E R Y
R E S E A R C H A T I S U & E D H
Spring 2009
I-SHOU UNIVERSITY E-DA HOSPITAL
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IEEE
(Institute of Electrical and Electronics Engineers IEEE)
(Institute of Electrical and
Electronics EngineersIEEE)
150365,000IEEE
IEEE(Fellow)
2009IEEE Fellow
2004(IMAPS) (IEEE)
(CPMT) 2007
IEEE
2008
70
150
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15
1 3 4 1 4 2
IEEE
IEEE
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4
2 0 0 7 Vo i c e
R e c o n s t r u c t i o n
f e l l o w
5
200811Live Demo
~
2007
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5Search & Discovery Research at ISU & EDA
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Department of Business Management
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Department of International Finance
Department of Tourism & Hospitality
http://www.isu.edu.tw/admission
07-65777112133
Back
http://www.isu.edu.tw/admission -
7Search & Discovery Research at ISU & EDA
(
)
()
5
10-4 mg/ml
1121
1 2
()
-
8
RGD (Arg-Gly-Asp)
DGEA (Asp-Gly-Glu-Ala)
RGD (Arg-
Gly-Asp)DGEA (Asp-Gly-Glu-Ala)
motifs
fibronectin
Arginine-Glycine-Aspartate (RGD)
lamininthrombinfibrinogenvitronectin
()
1.
(-1
)
forma
TEM 2%Tungstophosphoric acid
hydrate
(Philip CM-200)
2.
Seglen
SD
-
9Search & Discovery Research at ISU & EDA
-1
0.1% EDTA-Hank
37CHank
0.5%(
)
(500rpm, 5 mins)
3.
KCl
protease (2 mg/ml)2
proteoglycans
(2mg/ml)3
1500 rpm 10
F-12 (10%200 U/mL
(penicillin/streptomycin))
75T
F-12
4.
5104 cells/ml510-4
mg/ml50 ml
3
15 rpm
MTT
5.
5104 cells/ml10-4 mg/
ml50 ml
3
15 rpm
MTT
SEM6.
2.5% 2
1% 1
-
10
-2
-3
7.
-20CQuantiChromTM
BCP Albumin Assay kitELISA610
nm
8.
Blyscan Assay Kit
100 l1 mlDye reagent
30
Dissociation reagent 1ml
20 ELISA 650nm
9.
4%
5 m-Masson
1.
(-2-3)
37C
(-4)
-4
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11Search & Discovery Research at ISU & EDA
-5
2.
MTT
7250%
10
(
-5)
5
mm(-6)
(-7-8)
3.
(10-4 mg/ml)
(-9)
(-10)
(glycosaminoglycan)
glycosaminoglycan
(-11)
-6
5 mm
-7
-
12
-8
-10
-11
MTT
-9
2
-
13Search & Discovery Research at ISU & EDA
(
)
1. Ruiyun Zhanga nd Peter X. Ma, Synthetic
nano-fibrillar extracellular matrices with prede-
signed macroporous architectures, Journal of
Biomedical Materials Research, 52(2): 430-438,
2000.
2. C. H. Lee, Y. J. Wang, S. M. Kuo and S. J.
Chang, Microencapsulation of parathyroid tissue
with photosensitive poly(l-lysine) and short chain
alginate-coMPEG, Artificial Organs, 28(6):
537-542, 2004.
3. S. J. Chang, G. C. C. Niu, S. M. Kuo, C. C. Ho
and M. S. Bair, Preparation of Nano-Sized Parti-
cles from Collagen II by a High-Voltage Electro-
static Field System, Nanobiotechnology, 153(1):
1-6, 2006.
4. Laurence Besseau, Bernard Coulomb, Corinne
Lebreton-Decoster and Marie-Madeleine Giraud-
Guille, Production of ordered collagen matrices
for three-dimensional cell culture, Biomaterials,
23: 2736, 2002.
5. S. M. Kuo, S. J. Chang, L. C. Lin and C. J.
Chen, Evaluating chitosan/-tricalcium phos-
phate/poly(methyl methacrylate) cement compos-
ites as bone-repairing materials, Journal of Ap-
plied Polymer Science, 89(14): 3897-3904,2003.
6. L. C. Lin, S. J. Chang, S. M. Luo, G. C. C.
Niu, H. K. Keng and P. H. Tsai, Preparation and
Evaluation of -TCP /Polylactide Microspheres
as Osteogenesis Materials, Journal of Applied
Polymer Science, 108(5): 3210-3217, 2008.
7. S. J. Chang, G. C. C. Niu, S. M. Kuo and S. F.
Chen, Preparation and Preliminary Character-
ization of Concentric of Multi-Walled Chitosan
Microspheres, Journal of Biomedical Materials
Research Part A, 81A(3): 554-566, 2007.
8. Hideki Murakami, Masao Kobayashi, Hiro-
fumi Takeuchi and Yoshiaki Kawashima, Further
application of a modified spontaneous emulsifi-
cation solvent diffusion method to various types
of PLGA and PLA polymers for preparation of
nanoparticles, Powder Technology, 107: 137
143, 2000.
9. X. Y. Guo, J. E. Gough, P. Xiao, J. Liu and Z.
J. Shen, Fabrication of nanostructured hydroxy-
apatite and analysis of human osteoblastic cel-
lular response, Journal of Biomedical Materials
Research Part A, 82A(4): 1022-1032,2007.
10. S. M. Kuo, G. C. C. Niu, S. J. Chang, C. H.
Kuo and M. S. Bair, A one-step method for fab-
ricating chitosan microspheres, Journal of Ap-
plied polymer science, 94(5): 2150-2157, 2004.
Back
-
14
FAK
MTT
-
15Search & Discovery Research at ISU & EDA
//
/
()
FAK
1.
KCl
pronase E (2mg/ml)2
proteoglycanscollagenase II(2mg/
ml)3
collagenase
solution 1500rpm10
F-12 medium(10%200 U/
mL (penicillin/streptomycin))
75T flask
PBSF-12 medium
2.
-
16
-1.
TEM
formaTEM
2%Tungstophosphoric acid hydrate
(Philip CM-200)
3.
0.25%
trypsin15ml
5104 cells/ml
F-12 medium
10-210-310-410-5 mg/ml
50 ml
3
3
24
3
15rpm
MTT
glycosaminoglycan
4.
MTT
1ml
1 mLMTT
(0.5 mg MTT/mL)37
MTT
500 L DMSOvortex
200 Lformazan
DMSO570 nm
650 nm
5. Glycosaminoglycan
glycosaminoglycanAlcian blue
pH1.0
0.1NAlcian blue(Sigma)
pH1.00.1N
1
-
17Search & Discovery Research at ISU & EDA
2245.4-9
kVcm-1
1
-2 (a) ; (b)
MTT
Alcian blue
10-4 mg/ml
(3)Alcian blue
(4)
10-4 mg/ml
10-2 mg/ml
(5)
(10-2 mg/ml)
2 mmSEM
(6)
-3.
MTT
-
18
-5. , (a) ,(b)
10-2 mg/ml, (c),(d) 10-4 mg/ml.
-4. Alcian blue(a) Control (150 X). (b)
10-2 mg/ml. (c) 10-3 mg/ml. (d) 10-4 mg/ml. (e) 10-5 mg/ml.
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19Search & Discovery Research at ISU & EDA
SEM
2 mm
ControlCollagen II nano particles
10-2 mg/ml
Collagen II nano particles
10-4 mg/ml
400X 400X 400X
3000X 3000X 3000X
-6.
10-4 mg/ml
(10-2 mg/ml10-3 mg/ml
10-5 mg/ml)
SEM
-
20
1. Burgering BMT, Bos JL (1995) Regulation of
Ras-mediated signalling: more than one way to
skin a cat. Trends Biochem Sci 20:18-2
2. Kimura T, Yasui N, Ohsawa S, Ono K.
Chondrocytes embedded in collagen gels
maintain cartilage phenotype during long-term
cultures. Clin Orthop Relat Res 1984;186:231-9.
3. Kolettas E, Buluwela L, Bayliss MT, Muir
HI. Expression of cartilage-specific molecules is
retained on long-term culture of human articular
chondrocytes. J Cell Sci 1995;108:1991-9.
4. Hauselmann HJ, Fernandes RJ, Mok SS,
Schmid TM, Block JA, Aydelotte MB, et
al. Phenotypic stability of bovine articular
chondrocytes after long-term culture in alginate
beads. J Cell Sci 1994;107:17-27.
Back
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21Search & Discovery Research at ISU & EDA
7250%
10
5mm
()
[1-3]
3-D
[4]
[5-7]
[8-11]
-
22
--[12-14]
[15,16]
[17]
0.1 mg/ml
1 mL
18 cm 6 cm 2 cm
71.5kV/cm
20l1.5%
104 cell/ml
24-well(
375% CO2)
MTT
PBS
1 mL MTT
(0.5 mg MTT/mL)37
MTT
500 L DMSOvortex
formazan 200
L formazan DMSO
(ELISAenzyme-linked immunosorbentassay)
570 nm
650 nm
-2010
SD
Seglen
0.1%EDTA-Hank
37 Hank
0.5%(
)
(500 rpm, 5mins)
PBS
-
23Search & Discovery Research at ISU & EDA
-1
-2
QuantiChromTM BCP Albumin Assay kit
ELISA
610nm
10%
5 m
-Masson
MTT
(-2)
(
-3)MTT
MTT72
50%10
(-1
)TEM
(37)
-
24
3-D
5mm(-4)
(-5)
-
Masson
SEM(
-6)
RGD (Arg-Gly-
Asp)DGEA (Asp-Gly-Glu-Ala)
-4 5
mm
-3
(
)
-
25Search & Discovery Research at ISU & EDA
-5
-6
5
5mm
(6250m
)
1. Jake A. Gilbert, Amanda E. Simpson, David
E. Rudnick, Dayle H. Geroski, Thomas M.
Aaberg and Henry F. Edelhauser, Transscleral
permeability and intraocular concentrations of
cisplatin from a collagen matrix, Journal of
Controlled Release, 89: 409417, 2003.
-
26
2. H. P. Hsu, J. M. Zanella, S. M. Peckham and
M. Spector, Comparing ectopic bone growth
induced by rhBMP-2 on an absorbable collagen
sponge in rat and rabbit models, Journal of
Orthopaedic Research, 24(8): 1660-1669, 2006.
3. J. Wang, I. L. Lee, W. S. Lim, S. M. Chia, H.
Yu, K. W. Leong and H. Q. Mao, Evaluation
of collagen and methylated collagen as gene
carriers, International Journal of Pharmaceutics,
279: 115126, 2004.
4. Laurence Besseau, Bernard Coulomb, Corinne
Lebreton-Decoster and Marie-Madeleine Giraud-
Guille, Production of ordered collagen matrices
for three-dimensional cell culture, Biomaterials,
23: 2736, 2002.
5. Robert B. Vernon, Michel D. Gooden,
Stephanie L. Lara and Thomas N. Wight, Native
fibrillar collagen membranes of micron-scale
and submicron thicknesses for cell support and
perfusion, Biomaterials, 26: 11091117, 2005.
6. D. I. Zeugolis, R. G. Paul and G. Attenburrow,
Extruded collagen-polyethylene glycol fibers
for tissue engineering applications, Journal of
Biomedical Materials Research Part B: Applied
Biomaterials, 85B(2): 343-352, 2008.
7. Ruiyun Zhanga nd Peter X. Ma, Synthetic
nano-fibrillar extracellular matrices with
predesigned macroporous architectures, Journal
of Biomedical Materials Research, 52(2): 430-
438, 2000.
8. C. H. Lee, Y. J. Wang, S. M. Kuo and S. J.
Chang, Microencapsulation of parathyroid tissue
with photosensitive poly(l-lysine) and short chain
alginate-coMPEG, Artificial Organs, 28(6):
537-542, 2004.
9. S. M. Kuo, S. J. Chang, L. C. Lin and C.
J. Chen, Evaluating chitosan/-tricalcium
phosphate/poly(methyl methacrylate) cement
composites as bone-repairing materials, Journal
of Applied Polymer Science, 89(14): 3897-
3904,2003.
10. L. C. Lin, S. J. Chang, S. M. Luo, G. C. C.
Niu, H. K. Keng and P. H. Tsai, Preparation and
Evaluation of -TCP /Polylactide Microspheres
as Osteogenesis Materials, Journal of Applied
Polymer Science, 108(5): 3210-3217, 2008.
11. S. J. Chang, G. C. C. Niu, S. M. Kuo and
S. F. Chen, Preparation and Preliminary
Characterization of Concentric of Multi-Walled
Chitosan Microspheres, Journal of Biomedical
Materials Research Part A, 81A(3): 554-566,
2007.
12. Jayanth Panyam, Manisha M. Dali, Sanjeeb
K. Sahoo, Wenxue Ma, Sudhir S. Chakravarthi,
Gordon L. Amidon, Robert J. Levy and Vinod
Labhasetwar, Polymer degradation and in vitro
release of a model protein from poly(D,L-lactide-
co-glycolide) nano- and microparticles, Journal
of Controlled Release, 92: 173187, 2003.
13. Hideki Murakami, Masao Kobayashi,
Hirofumi Takeuchi and Yoshiaki Kawashima,
Further application of a modified spontaneous
emulsification solvent diffusion method to various
types of PLGA and PLA polymers for preparation
of nanoparticles, Powder Technology, 107: 137
143, 2000.
14. X. Y. Guo, J. E. Gough, P. Xiao, J. Liu
and Z. J. Shen, Fabrication of nanostructured
h y d r o x y a p a t i t e a n d a n a l y s i s o f h u m a n
osteoblastic cellular response, Journal of
-
27Search & Discovery Research at ISU & EDA
Biomedical Materials Research Part A, 82A(4):
1022-1032,2007.
15. S. H. Hong, D. W. Lee and B. K. Kim,
Manufacturing of aluminum flake powder from
foil scrap by dry ball milling process, Journal of
Materials Processing Technology, 100: 105-109,
2000.
16. T. H. Hou, C. H. Su and W. L. Liu,
Parameters optimization of a nano-particle
wet milling process using the Taguchi method,
response surface method and genetic algorithm,
Powder Technology, 173: 153-162, 2007.
17. S. M. Kuo, G. C. C. Niu, S. J. Chang, C. H.
Kuo and M. S. Bair, A one-step method for
fabricating chitosan microspheres, Journal of
Applied polymer science, 94(5): 2150-2157,
2004.
Back
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(drug
delivery system, DDS)
11 (quantum dots, QDs)
21 (Fe3O4)
31 (Gold nanomaterials)
11 (quantum dots, QDs)
(-1)
CdSe2.1 nm
5 nm
10 nm
DNA
-
29Search & Discovery Research at ISU & EDA
-2
-
(-3)
(Fe3O4) 21
-4
6~8
-2QDs-PLGA() ()
-1 (CdSe/ZnS)
-
30
-4 Fe3O4(TEM)
-3 - (50X)
() ()
(1)
(2)
(3)
(4)
31 (Gold nanomaterials)
-4 (a)(b)
TEM(c)
Back
-
31Search & Discovery Research at ISU & EDA
(intestinal flap)
(radial forearm flap)
(free
colon flap)
(end-to-side anastomosis)
(free jejunal
flap)
(intussuseption)
(free ileocolon flap)
(manometry)
(technetium-99m)
(radionuclide esophageal scintigraphy)
26 (24
2)55
121
13
1011
-
32
(initial pharyngeal clearance)
(pharyngeal transit rate)
(61
20%70 16%) (-1)
(esophageal clearance)
(pharyngeal transit rate)
(
50 27%69 17% ) (-2)
0
20
40
60
80
100
(%)
0
20
40
60
80
100
(%)
-1:
-2:
1958Seidenberg
Hurwitt
90%100%
1992Kawahara
(cutaneous
flap) (musculocutaneous flap)
(segmentation contraction)
-
33Search & Discovery Research at ISU & EDA
(mass movement)
(dysphagia)
DantasMamede
Smith
(functional
stenosis)
Smith
:
Back
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2009 International Conference on Environmental Quality Concern
Control and Conservation200952223
-
http://www2.isu.edu.tw/upload/28/2/discovery/EQC.pdf
2009
2009619
-
RFID
http://www.isu.edu.tw/interface/overview.php?dept_mno=130
98420
2009 2009620
-
http://www.isu.edu.tw/itamc
98515
http://www2.isu.edu.tw/upload/28/2/discovery/EQC.pdfhttp://www.isu.edu.tw/interface/overview.php?dept_mno=130http://www.isu.edu.tw/itamc -
35
200941
A0102
200947
50916
2009411
200957
200959
2009415
50901
-
36
Context-aware Computing and its applications
2009416
3801
Bank Basics, Loan Loss Provision, Earnings Management
and Its Impacts
2009417
51301
2009423
10
http://www1csie1isu1edu1tw/news/shortclass1htm
/
200956
50901
2009515
51301
V2oIP/
2009521
3801
http://www.csie.isu.edu.tw/news/shortclass.htm -
37
2009521
2009527
50901
Back
-
38
2010 (NSC-ANR)
(ANR)
(Program BLANC)(Program
TecSan)
http://web1.nsc.gov.tw/
200942
98 ()
http://web1.nsc.gov.tw/
2009430
2010 2000
2010
http://web1.nsc.gov.tw/
2009531
2010 ()1989(The Swiss National
Science Foundation, SNSF)(The fonds zur Forderung der
wissenschaftlichen orschung, FWF)20022007
http://web1.nsc.gov.tw/
2009731
http://web1.nsc.gov.tw/http://web1.nsc.gov.tw/http://web1.nsc.gov.tw/http://web1.nsc.gov.tw/ -
39Search & Discovery Research at ISU & EDA
2009
(AAFC)
(CRPs)
http://web1.nsc.gov.tw/
2009731
(NSC-NRC)NRC
NRC
90NSC-NRC
5()
5NRC
http://web1.nsc.gov.tw/
2009731
98/99 (DAAD)
http://web1.nsc.gov.tw/
2009731
http://web1.nsc.gov.tw/
2009731
()
http://web1.nsc.gov.tw/http://web1.nsc.gov.tw/http://web1.nsc.gov.tw/http://web1.nsc.gov.tw/ -
40
http://web1.nsc.gov.tw/
2009731
2009
1. The Scripps Research Institute (La Jolla, CA)2. The Fred Hutchinson Cancer Research
Center (Seattle, Washington)3. California Institute of Technology (Pasadena, CA)4. Baylor
College of Medicine (Houston, TX) 5. The University of Texas M.D. Anderson Cancer Center
(Houston, TX)
http://web1.nsc.gov.tw/
http://www.isu.edu.tw/interface/showdoc.php?dept_id=4&levelid=4473&dept_mno=27
20093430
20082153
TDPA ---
http://www.isu.edu.tw/interface/showdoc.php?dept_id=4&levelid=5359&dept_mno=27
200811
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41Search & Discovery Research at ISU & EDA
AITI
http://sita.stars.org.tw/
2008121
SBIR () SBIR(Small Business Innovation Research)
http://www.sbir.org.tw/SBIR/Web/Default.aspx
()
http://leading.itnet.org.tw/index.php
()
http://sita.stars.org.tw/http://www.sbir.org.tw/SBIR/Web/Default.aspxhttp://leading.itnet.org.tw/index.php -
42
Back
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--
--
http://www.isu.edu.tw/interface/showdoc.php?dept_id=4&levelid=5586&dept_mno=27
http://www.isu.edu.tw/interface/showdoc.php?dept_id=4&levelid=5358&dept_mno=27http://www.isu.edu.tw/interface/showdoc.php?dept_id=4&levelid=5586&dept_mno=27 -
43Search & Discovery Research at ISU & EDA
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