S CIENTIFIC S TUDIES AND U NIVERSITY E XPERIMENTS.
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Transcript of S CIENTIFIC S TUDIES AND U NIVERSITY E XPERIMENTS.
SCIENTIFIC STUDIES AND UNIVERSITY
EXPERIMENTS
• Following are pictures of the surgical procedures and research conducted at Mahidol University, in Thailand
• All Thai research was coordinated by Assistant Professor Kampon Kaeoket, DVM, MSc, PhD (Pig Production); Dusit Laohasinnarong, DVM, and Dr. Jo Ann Hachuela of the Philippines.
Preparation and staging of animals for research
Ovulation detection using ultrasound
Administration of pre-anesthetic drugs
Administration of anesthetic
Sedated and anesthetized animal heading for surgery
Cleaning and disinfecting the surgical site
Properly restrained animal at beginning of surgery
Ovariohysterectomy in progress
Locating the reproductive tract
Exposing the uterine horns, oviducts, and ovulated ovaries
Ovulated ovaries
Removing the reproductive tract
Transferring the tract for flushing procedures
Separating the upper uterine horns, oviducts, and ovaries
Incising the corpora lutea and determining the number of ovulated oocytes
Ovaries, upper portion of uterine horns, and oviducts ready for flushing
Incising and separating the isthmus from the ampulla and infundibulum
Pre-labeled petri dishes for the oocytes to be recovered
Withdrawal of phosphate buffer saline solution for flushing
Flushing of the isthmus
Flushing of the ampulla and infundibulum
Flushing of the upper uterine horn
Stereo microscopes for examining the oocytes
Prof. Kampon Kaeoket, D.V.M., M.S. (Obstetrics and Gynecology), Ph.D.(Obstetrics and Gynecology, Pig Reproduction) examining fertilized vs. non-fertilized oocytes
Isolating the oocytes to maintain accuracy during counting
Further examination…
Click the document to open a .PDF file showing actual evaluation protocols used for the Mahidol University Trial.
Click the document to open a .PDF file showing the surgical and on farm trial results from Thailand.