Results - orbi.uliege.be©Maes.pdf2Gembloux Agro-Bio Tech, Universitéde Liège, 2 Passage des...
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Context The risks coming from the use of pesticides are one of the major controversies these days. Indeed, more and more environmental and public health problems are detected following the use of these products 1 . Essential oils are prime candidates to create ecological alternatives. However, the high volatility of these compounds induces the needing to encapsulate these. Following this, a certain number of encapsulation techniques have already been performed, but the gradual release time necessary for the proper functioning of a plant protection product has not yet been optimized. This is where our work begins. The innovative encapsulation of essential oils is realized using dendrimers, which are macromolecules with a tree structure, which gives them an encapsulation capacity. In addition, the specimens used contain a substitute of natural origin: glycerol. The potential of dendrimers to encapsulate essential oils Chloé Maes 1 , Sandrine Bouquillon 1 , Marie-Laure Fauconnier 2 1 Institut de Chimie Moléculaire de Reims, UMR CNRS 7312, Université Reims-Champagne-Ardenne, UFR Sciences, BP 1039 boîte 44, 51687 Reims Cedex 2, France 2 Gembloux Agro-Bio Tech, Université de Liège, 2 Passage des Déportés, 5030 Gembloux, Belgique Synthesis Dendrimer structure Objectives In a first step, the encapsulation capacity of different dendrimers was studied. Then, the optimal conditions of the reaction and a complete analysis of the interactions between the dendrimers and the essential oils will be carried out. In a second step, a study of the release of each system will be performed to create an effective biological pesticide. + Et 3 N/MeOH 18h Yield : 82% Contact : [email protected] Encapsulation ` Results Dendrimer Encapsulated product Yield GD-PPI-4 β-estradiol 62,66 % 3 GD-PPI-4 Atrazin 62,47 % 3 GD-PPI-4 Diuron 58,65 % 3 GD-PPI-4 Diclofenac sodium salt 36,41 % 3 GD-PPI-4 Gadopentetate dimeglumine 36,32 % 4 GD-PPI-4 Citronella essential oil 18,27 % GD-PAMAM-3 β-estradiol 72,03 % 3 GD-PAMAM-3 Diuron 61,39 % 3 GD-PAMAM-3 Atrazin 60 % 3 GD-PAMAM-3 Diclofenac sodium salt 23,63 % 3 GD-PAMAM-3 Citronella essential oil 16,32 % Introduction + Dendrimer Product to encapsulate agitation Liquid/Liquid extraction Lyophilisation Perspective: DHS-GC-MS Analysis + Dendrimer Product to encapsulate Optimisation of: - Type of dendrimer - Temperature - Stirring rate - Concentrations - Stirring duration 1. Yield of total retention 2 r % = 1− ∑( ) ∑( + x100 A D = Area with dendrimers A 0 = Area without dendrimers 2. Retention yield of the essential oil's majors compounds (external calibration) Conclusion. This study shows that essential oil encapsulation by dendrimers is possible and need to be optimized. A very precise analytic method of dynamic headspace gas chromatography with mass spectroscopy will be used to precisely optimize parameters of the encapsulation of different type of dendrimers. Acknowledgments Authors thanks the University of Reims Champagne-Ardenne and the University of Liege for the financial support. Thanks to the technical staff of both universities for their availibility and their help. Literatures 1. Bruggen, V., & Jr, J. (2017) Science of the Total Environment, 616617, 255–268 2. Kfoury, M., Auezova, L., Greige-Gerges, H., & Fourmentin, S. (2015) Carbohydrate Polymers, 131, 264–272. 3. Menot, B., Stopinski, J., Martinez, A., Oudart, J. B., Maquart, F. X., & Bouquillon, S. (2015) Tetrahedron, 71(21), 3439–3446. 4. Balieu, S., Cadiou, C., Martinez, A., Nuzillard, J. M., Oudart, J. B., Maquart, F. X., Chuburu F., & Bouquillon, S. (2013) Journal of Biomedical Materials Research - Part A, 101 A(3), 613–621 Polyfonctional core Branching units Terminal groups PolyPropylenImine-4 Glycerodendrimer- PolyPropylenImine-4 Glycerol carbonate
Transcript of Results - orbi.uliege.be©Maes.pdf2Gembloux Agro-Bio Tech, Universitéde Liège, 2 Passage des...
ContextThe risks coming from the use of pesticides are one of the major controversies these days. Indeed, more andmore environmental and public health problems are detected following the use of these products1. Essentialoils are prime candidates to create ecological alternatives. However, the high volatility of these compoundsinduces the needing to encapsulate these.Following this, a certain number of encapsulation techniques have already been performed, but the gradualrelease time necessary for the proper functioning of a plant protection product has not yet been optimized.This is where our work begins. The innovative encapsulation of essential oils is realized using dendrimers,which are macromolecules with a tree structure, which gives them an encapsulation capacity. In addition, thespecimens used contain a substitute of natural origin: glycerol.
The potential of dendrimers to encapsulate essential oilsChloé Maes1, Sandrine Bouquillon1, Marie-Laure Fauconnier2
1Institut de Chimie Moléculaire de Reims, UMR CNRS 7312, Université Reims-Champagne-Ardenne, UFR Sciences, BP 1039 boîte 44, 51687 Reims Cedex 2, France2Gembloux Agro-Bio Tech, Université de Liège, 2 Passage des Déportés, 5030 Gembloux, Belgique
Synthesis
Dendrimer structure
ObjectivesIn a first step, the encapsulation capacity of different dendrimers was studied. Then, the optimal conditions ofthe reaction and a complete analysis of the interactions between the dendrimers and the essential oils will becarried out. In a second step, a study of the release of each system will be performed to create an effectivebiological pesticide.
+Et3N/MeOH
18h
Yield : 82%
Contact : [email protected]
Encapsulation`
Results
Dendrimer Encapsulated product YieldGD-PPI-4 β-estradiol 62,66 %3
GD-PPI-4 Atrazin 62,47 %3
GD-PPI-4 Diuron 58,65 %3
GD-PPI-4 Diclofenac sodium salt 36,41 %3
GD-PPI-4 Gadopentetate dimeglumine 36,32 %4
GD-PPI-4 Citronella essential oil 18,27 %
GD-PAMAM-3 β-estradiol 72,03 %3
GD-PAMAM-3 Diuron 61,39 %3
GD-PAMAM-3 Atrazin 60 %3
GD-PAMAM-3 Diclofenac sodium salt 23,63 %3
GD-PAMAM-3 Citronella essential oil 16,32 %
Introduction
+
Dendrimer Product toencapsulate
agitation Liquid/Liquid extraction
Lyophilisation
Perspective: DHS-GC-MS Analysis
+
Dendrimer Product toencapsulate
Optimisation of:- Type of dendrimer- Temperature- Stirring rate- Concentrations- Stirring duration
1. Yield of total retention2
r % = 1 − ∑ ()��∑ (+��
x100AD = Area with dendrimersA0 = Area without dendrimers
2. Retention yield of the essential oil's majors compounds (externalcalibration)
Conclusion.This study shows that essential oil encapsulation by dendrimers is possible and need to be optimized. A very precise analytic method ofdynamic headspace gas chromatography with mass spectroscopy will be used to precisely optimize parameters of the encapsulation ofdifferent type of dendrimers.
AcknowledgmentsAuthors thanks the University of Reims Champagne-Ardenne and theUniversity of Liege for the financial support.
Thanks to the technical staff of both universities for their availibility andtheir help.
Literatures1. Bruggen, V., & Jr, J. (2017) Science of the Total Environment, 616617,
255–2682. Kfoury, M., Auezova, L., Greige-Gerges, H., & Fourmentin, S. (2015)
Carbohydrate Polymers, 131, 264–272.3. Menot, B., Stopinski, J., Martinez, A., Oudart, J. B., Maquart, F. X., &
Bouquillon, S. (2015) Tetrahedron, 71(21), 3439–3446. 4. Balieu, S., Cadiou, C., Martinez, A., Nuzillard, J. M., Oudart, J. B., Maquart,
F. X., Chuburu F., & Bouquillon, S. (2013) Journal of Biomedical MaterialsResearch - Part A, 101 A(3), 613–621
Polyfonctional coreBranching unitsTerminal groups
PolyPropylenImine-4Glycerodendrimer-PolyPropylenImine-4
Glycerolcarbonate
