REGIONAL REFERENCE LABORATORY FOR FMD IN · PDF fileDEPARTMENT OF LIVESTOCK DEVELOPMENT...

University of Tokyo, Tokyo, Japan, 9-11 June 2015

REGIONAL REFERENCE LABORATORY FOR FMD IN SOUTH EAST ASIADEPARTMENT OF LIVESTOCK DEVELOPMENT

PAKCHONG, NAKHONRATCHASIMA, THAILAND

Activities of FMD Laboratory and

Networking

OIE Reference Laboratory for FMD,

Pakchong, Thailand

The Foot and Mouth Disease (FMD) Laboratory in PAKCHONG, was first established in 1958

The South East Asia Foot and Mouth Disease (FMD) Regional Reference Laboratory (RRL) in Pakchong, Thailand was accredited as an OIE Reference Laboratory for FMD since 2009.

The DLD refurbished laboratory capacity by constructing a BSL-3 Containment Laboratory.

The BSL-3 laboratory has been designated as the SEAFMD RRL and was officially opened in 11 October 2004, and since then has received samples from South East Asian countries.

1. Regional: pool -1

2. Period of coverage: 2014-2015

3. Countries covered:

- Cambodia - Laos PDR

- Myanmar - Malaysia

- Vietnam - Indonesia

- Brunei - Singapore

- Philippines - Thailand

Source : OIE SRR-SEA

FMD Free Zone

FMD Infected Areas

Present : FMD Free countries / Zones

- Philippine : FMD free whole

country

without vaccination

- Brunei

- Indonesia

- Singapore

- Malaysia : Free in Sabah and

Salawak

•

Tissue sample submission to RRL: Antigen Detection During 2014 - 2015

Year Country No. of sample

(Tissue)

ELISA typing results RT-PCR results

O A Asia1 NVD Positive Negative

2014

Cambodia 17 5 - - 12

7

(Type 0=6,

Type A=1)

10

Lao PDR 5 - 3 - 2 4 1

Thailand 355 88 116 - 151 269 86

2015 Thailand 76 40 11 - 25 62 14

NVD = No virus detected

Serum sample received in 2014-2015

Country

2014 2015

LP ELISA

NSP s Test

NSPs test LP

ELISA NSPs Test

NSPs test

positive negative (% positive) positive negative (% positive)

Thailand 12,372 3,590 172 3,424 4.78 7,236 2,796 171 2,625 6.11

Interpretation r-value

Ref. WRL and Samuel et al., 1990

Vaccine matching of FMDV type O in SEA region in 2013-2015 (Homologous using O Udornthani 189/87 Thai vaccine strain)

Country YearTotal

sample

Range of r-value by LP ELISA

0-0.19

Poor Matching

0.2-0.39

moderate matching

0.4-1.0

Good Matching

Cambodia 2013

2014

5

1

-

-

-

-

5

1

Thailand 2013

2014

2015

17

16

13

-

-

-

-

1

-

17

15

13

Total 52 - 1 (1.92%) 51 (98.08%)

Vaccine matching or r-value of FMDV type A

in SEA region in 2013-2015

Country YearTotal

sample

Range of r-value by LP ELISA test

A118/87 A/Sakolnakorn/97 A/Lopburi/2012

0.2-0.39 0.4-1.0 0.2-0.39 0.4-1.0 0.2-0.39 0.4-1.0

Thailand

Lao PDR

Vietnam

2013

2014

2015

2014

2013

13

16

4

3

3

- 4

2

1

-

1

-

14

15

4

2

3

No binding reaction

No binding reaction

No binding reaction

No binding reaction

No binding reaction

No binding reaction

No binding reaction

No binding reaction

No binding reaction

Sample IDs

A Lop Buri 2012 A22 IRQ 64 A MAY 97

Homo Hetero ‘r’ value Homo Hetero‘r’

valueHomo Hetero

‘r’

value

VIT/1/13 B3 3.16 3.16 1.00 3.37 2.75 0.24 3.78 3.38 0.40

VIT/2/13 B3 3.16 2.86 0.50 3.59 3.11 0.34 4.08 3.02 0.09

VIT/3/13 B3 3.46 3.16 0.50 3.14 3.18 >1.00 4.38 3.34 0.09

TAI/20/13 R3B3 3.76 3.46 0.50 3.12 2.88 0.58 4.05 2.32 0.02

TAI/26-3/13 R3B2 3.76 3.46 0.50 3.39 4.08 >1.00 4.69 2.33 0.00

TAI/39/13 R1B2 3.16 2.98 0.66 3.22 2.78 0.36 4.20 2.58 0.02

TAI/47-2/13 R1B2 3.16 2.98 0.66 3.78 2.47 0.05 4.00 3.02 0.10

TAI/53/13 R2B2 3.16 2.98 0.66 3.80 2.72 0.08 3.72 3.08 0.23

TAI/58/13 R3B2 3.16 2.98 0.66 3.61 2.45 0.07 3.60 3.15 0.35

TAI/60/13 R1B2 3.46 3.58 >1.00 3.47 2.46 0.10 4.12 3.33 0.16

‘r’ value estimates

‘r’ value estimates

Sample IDs

A Lop Buri 2012 A22 IRQ 64 A MAY 97

Homo Hetero ‘r’ value Homo Hetero‘r’

valueHomo Hetero

‘r’

value

LAO/2/14 B4 2.56 2.68 >1.00 3.01 2.49 0.30 4.16 3.67 0.33

LAO/5/14 B3 2.56 2.68 >1.00 3.14 2.57 0.27 3.66 3.98 >1.00

TAI/1-2/14 B3 2.56 2.68 >1.00 3.35 2.95 0.39 4.52 2.98 0.03

TAI/2/14 B3 2.56 2.68 >1.00 3.13 2.57 0.27 4.56 3.20 0.04

TAI/5/14 B1 2.56 2.68 >1.00 3.19 2.52 0.21 3.88 3.82 0.88

TAI/10/14 R2B3 2.56 2.68 >1.00 3.57 2.69 0.13 3.83 3.01 0.15

TAI/13-2/14 B4 2.56 2.68 >1.00 3.58 2.38 0.06 4.81 2.74 0.01

TAI /21-2/14 B4 3.46 3.58 >1.00 2.99 2.85 0.73 4.67 3.04 0.02

TAI/44/14 B4 3.16 2.98 0.66 4.00 2.58 0.04 3.60 2.90 0.20

TAI/65-1/14 R1B2 - - - 3.35 2.75 0.25 3.95 2.74 0.06

Vaccine Matching from WRL

CirculatingSerotype

Internationally available vaccines

Locally produced vaccine

O

O Tur 5/09O TAW 98O 3039O1 Manisa

Thailand, O Udornthani 189/87

AA22 IraqA Tur06

A Malaysia 97

Thailand, A Lopburi/12A Sakolnakorn/97

Asia 1 Asia1 Shamir Thailand Asia1/85

Phylogenetic tree of FMDV type O outbreak in South East Asia 2014- 2015

Indicates viruses in this report in 2014


Report by Regional Reference Laboratory

for FMD in South East Asia, Pakchong,

Nakhonratchasima, Thailand

Phylogenetic tree of FMDV type A outbreak in South East Asia 2014- 2015



Report by Regional Reference Laboratory

for FMD in South East Asia, Pakchong,

Nakhonratchasima, Thailand

FMDV 2014 2015

Type O

SEA topotype(Mya-98 strain)

SEA topotype(Mya-98 strain)

Type A

ASIA topotype(Sea-97 strain)

ASIA topotype(Sea-97 strain)

Summary In 2014, serotype A/Sea-97 is predominant in

Thailand and Lao PDR, serotype O/Mya-98 in

Cambodia.

FMD serotype A in 2014 belonged to ASIA

topotype, Sea-97 strain.

FMD serotype O in 2014 belonged to SEA

topotype, Mya-98 strain.

New lineage of serotype A is observed.

No Asia1 detected in 2014

National Institute of Animal Health (Bangkok)

VRDC = Veterinary Research and Development Center

VRDC- Upper northern (Lampang)

VRDC- Lower northern (Pitsanuloke)

VRDC- Upper northeastern (KhonKhaen)

VRDC- Lower northeast (Surin)

VRDC- ER (Chonburi)

VRDC- Western (Ratchaburi)

VRDC- Southern 1 (Nakhon Sri Thammarat)

VRDC- Southern 2 (Songkhla)

RRL has been accredited for ISO/IEC 17025:2005from Accreditation Body in Thailand, Bureau of Laboratory Quality

Standard, Dept. of Medical Science

Accreditation scopes:

- Antigen typing test by ELISA technique

- Antibody measurement by LP ELISA

- 3B Non structural protein (NSP) test

- 3ABC Non structural protein (NSP) test

International Quality Standard

Inter laboratory comparison round 4/2014-2015Participating lab: 18 labs

8 FMD laboratories within Thailand; VRDC _ Western part (Ratchaburi) VRDC _ Upper Northern (Lumpang) VRDC _ Lower Northern (Pisanuloke) VRDC _ Upper North Eastern (Khonkean) VRDC _ Lower North Easter (Surin) VRDC _ Eastern part (Chonburi) VRDC _ Southern Part (Nakhon Sri Thammarat) National Institute of Animal Health (Bangkok)

10 SEAFMD laboratories; Cambodia, Lao PDR, Myanmar, Thailand (RRL)

Vietnam (Hanoi), Vietnam (Ho Chi Minh), Singapore,Philippines Brunei, Malaysia , ( in logistic process)

* Conducted the 4th inter-laboratory comparison testing on FMD serology and antigen typing test, organized by RRL (Nov. 2014-May 2015)

- Rabbit trapping antibody for FMDV type O, A and Asia1

- Guinea pig detecting antibody for FMDV type O, A and Asia1

- Concentrated Inactivated FMD antigen type O, A and Asia1

- Control serum : strong and weak positive serum, negative serum

Number of FMDV diagnostic reagents and reference materials from RRL FMD supplied for nationally and OIE member countries in 2014-2015

Type of reagents Supplied nationally

and RRLSupplied OIE

members countriesTotal Remarks

Rabbit trapping antibody for type O, Aand Asia1

Type O = 16 setsType A = 21 setsType Asia1 = 20 sets

Type O = 13 setsType A = 13 setsType Asia1 = 13 sets(Laos, Myanmar, Cambodia, Vietnam)


1 set can be tested for 500 samples

Guinea pig detecting anibody for type O, A and Asia1




1 set can be tested for 500 samples

Concentrated inactivated (50X) antigen type O, A and Asia1

Type O = 94 mlType A = 68 mlType Asia1 = 46 ml



Control serum for C++, C+ and C-

C++ = 180 mlC+ = 180 mlC- = 180 ml

C++ = 155 mlC+ = 155 mlC- = 155 ml

C++ = 335 mlC+ = 335 mlC- = 335 ml

Collaborators Purpose of collaboration Outcomes

- Australian Animal Health Laboratory

- Regional Reference Laboratory for FMD in south East Asia

Foot and Mouth Disease risk Management for Australia and South East Asia (2014-2016)

Laboratory capacity building through training and transfer of disease diagnosis capabilities for regional virus circulation in SEA region.

- Bureau of Veterinary Biologics- Bureau of Disease Control and

Veterinary Services- Regional Reference Laboratory

for FMD in south East Asia

Study of the efficacy of FMD vaccine and vaccination program in Thailand

To improve the vaccination program in Thailand and induce the higher herd immunity for FMD

- Regional Reference Laboratory ffor FMD in south East Asia

- Kasetsart University, Thailand

Development of recombinant 3ABC-based ELISA to differentiate vaccinated from FMD infected animals.

To have a new NSP test kit used in Thailand at a lower cost.

Training OIE member country in 2014-2015

Myanmar, workshop on PCR for FMD Diagnosis3-27 June 2014

Australia, vaccine matching21 July – 1 August 2014

Vietnam, Cambodia and Lao PDR, Laboratory Training for FMD Diagnosis15-26 December 2014

Australia, vaccine matching20 April – 1 May 2015

JICA preparatory survey team, 12 May 2014

Dr.Pham Van Dong, DAH Director General, CVO and Delegates, Vietnam28 July 2014

Animal, Plant and Fisheries Quarantine and Inspection Agency, Republic of Korea14 November 2014

Acknowledgements: RRL staff OIE-SRR SEA

Thank you for your attention

REGIONAL REFERENCE LABORATORY FOR FMD IN · PDF fileDEPARTMENT OF LIVESTOCK DEVELOPMENT...

Documents

Transcript of REGIONAL REFERENCE LABORATORY FOR FMD IN · PDF fileDEPARTMENT OF LIVESTOCK DEVELOPMENT...