Recombiant protein technology

Emerging Food Technologies Presentation Series - 2016

University Of Sri Jayewardanepura

RECOMBINANTPROTEIN

TECHNOLOGYDinindu Herath

AS 2013707

3rd Year – 1st Semester

1st Group

13.03.2016



Dinindu Herath From Royal College Colombo

Interested in Dancing, Singing & Acting

Having passion to work cohesively with peers and lead them for achieve goals.

Currently ,

•Vice President of the Career Skills &

Development Society

•Member of the University Taekwondo Team

•Compiler of the “Concord forever” Blog



Do you know ……. ?

•Testing for genetic diseases

•DNA fingerprinting

•Commercial Products Eg:- recombinant insulin, HGH

•Genetic engineering of Plants and animals

•Cloning of organisms

•Gene therapy



Do you know ……. ?

•Testing for genetic diseases

•DNA fingerprinting

•Commercial Products Eg:- recombinant insulin, HGH

•Genetic engineering of Plants and animals

•Cloning of organisms

•Gene therapy

Recombinant Protein Tech



What is a Recombinant Protein…. ?

It is Protein that whose code is carried by a

Recombinant DNA

RECOMBINANT DNA ?????



Recombinant DNA means …….

DNA that has been formed artificially by combining constituents From different organisms.



How to make Recombinant Proteins…. ?

• Molecular Cloning - laboratory method used to make recombinant DNA

• Polymeraze Chain Reaction method



Molecular Cloning ……..



PCR technique……..



APPLICATIONSEnvironmental conservation

Agriculture

Bioremediation

Genetically Modified Organisms

Recombinant Vaccines



PROS & CONSMedical Benefits

Agricultural Benefits

Health Problems

Ethical concerns

Cost and Expertise

Every Technology comes with its Advantages and ConcernsEg :- Fire, Nuclear power , Electricity



In Srilanka…….

Various components of biotechnology are being carried out in several institutes with funds obtained from local agencies, such as

•National Science Foundation (NCF)

•Council for Agricultural Research Policy (CARP)

•National Research Council (NRC)



Using Recombinant DNA Technology …….. In SL

•Production of higher yielding crops resistant to pests diseases

•Organisms with novel capabilities Eg :- Cows producing pharmaceuticals in their milk•Cotton carrying bacterial genes providing resistance to insect attack•Golden rice with high vitamin A content•Edible vaccines in potatoes and bananas•Low cholesterol oils•Production of pharmaceuticals and nutraceuticals by bacteria



THANK YOU…..



References Safe Biotechnolog For Srilanka by Prof .Athula Perera , Agricultural Biotechnology Centre, University of Peradeniya

Recombinant Proteinswww.innovateus.net

Recombiant protein technology

Education

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