RECIPE Charquemont Progress meeting 23 - 26/10/2003
description
Transcript of RECIPE Charquemont Progress meeting 23 - 26/10/2003
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Craigiebuckler, Aberdeen, AB15 8QH, UK
RECIPE Charquemont Progress meeting
23 - 26/10/2003
UK progress
Rebekka Artz
Stephen Chapman
Colin Campbell
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I. WP01 UK Field site
• Middlemuir Moss, between Strichen and New Pitsligo, NE Scotland
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Bare milled peat (0-5 yrs)
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1st succession peat (> 5yrs)
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Regenerating peat (> 50 yrs)
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> 50 yrs 5 -10 yrs Bare
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Table 1. Approximate species distribution at RECIPE WP01 field sites
Experimental Site / Keystone species
Plant distribution (%)
Collar 1 Collar 2 Collar 3
Bare milled surface
bare bare bare
1st Succession Peat / Eriophorum
EA (80) SF (15) EV (<5) CI (<1)
EA (80)SF (15)EV (5)
EA (70)SF (20)EV (5)CS (3)CI (2)
1st Succession Peat / Sphagnum
SF (100) SF (95) EA (5)
SF (95) EA (5)
Regenerating Peat / Polytrichum
PC (80)SC (10)HS (3)PS (3)CS (4)
PC (75)SM (5)SC (10)SCU (5)CS (5)
PC (95)SM (<1)SC (<4)O
Regenerating Peat / Sphagnum
SC (80)SCU (15)SM (5)
SC (60)SCU (15)SM (5)HS (10)PS (5)CS (<5)O
SC (70)SCU (15)SM (5)PS (5)CS (<5)O
Key: EV – Eriophorum vaginatum,EA – Eriophorum angustifolium,SF – Sphagnum fallax,SM – Sphagnum magellanicum,SC – Sphagnum capillifolium,SCU – Sphagnum cuspidatum,CI – Campylopus introflexus,PC – Polytrichum commune, HS – Hypnum sp. (jutlandicum or cypressiforme),PS – Pleurozium schreberi, CS – Calluna sp., O – others (grasses etc.)
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II. Field site preliminary data
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Water table in plotsBare peat
-120
-100
-80
-60
-40
-20
0
0 10 20 30 40 50 60 70
time (d)
wat
er t
able
(cm
)
well 1 well 2 well 3 1st succession peat
-120
-100
-80
-60
-40
-20
0
0 10 20 30 40 50 60 70
time (d)
wa
ter
tab
le (
cm
)
well 1 well 2 well 3
Regenerating peat
-120
-100
-80
-60
-40
-20
0
0 10 20 30 40 50 60 70
time (d)
wat
er t
able
(cm
)
well 1 well 2 well 3
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Chamber leak test - no. I
0
200000
400000
600000
800000
1000000
1200000
1400000
1600000
1800000
-3 0 3 6 9 12 15 18 21 24 27 30 33 36 39 42 45 48
time (min)
(CH
4 ar
ea)
Methane added
Field measurements of CO2 and CH4 fluxes
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Bare peat – CO2
DARK 25/09
0
0.01
0.02
0.03
0.04
0.05
0.06
0.07
0 5 10 15 20 25 30
time (min)
CO
2 (%
)
bare 1 bare 2 bare 3 DARK 16/10
0
0.01
0.02
0.03
0.04
0.05
0.06
0.07
0 5 10 15 20 25 30
time (min)
CO
2 (%
)
bare 1 bare 2 bare 3
- 41 cm- 90 cm
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Bare peat – CH4
DARK 25/09
0
1
2
3
4
5
0 10 20 30time (min)
CH
4 in
pp
m
collar no 1 collar no 2 collar no 3
- 90 cm
DARK 16/10
0
1
2
3
4
5
0 5 10 15 20 25 30
time (min)
CH
4 in
pp
m
bare 1 bare 2 bare 3
- 41 cm
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1st succession peat – CO2
DARK 25/09
0
0.02
0.04
0.06
0.08
0.1
0.12
0.14
0.16
0.18
0 5 10 15 20 25 30time (min)
CO
2 (%
)
Sph 1 Sph 2 Sph 3 Erio 1 Erio 2 Erio 3
- 22 cm
DARK 16/10
0
0.01
0.02
0.03
0.04
0.05
0.06
0.07
0 5 10 15 20 25 30
time (min)
CO
2 (%
)
Sph 1 Sph 2 Sph 3 Erio 1 Erio 2 Erio 3 LIGHT 16/10
0
0.01
0.02
0.03
0.04
0.05
0.06
0.07
0 5 10 15 20 25 30time (min)
CO
2 (%
)
Sph 1 Sph 2 Sph 3 Erio 1 Erio 2 Erio 3
- 7 cm - 7 cm
LIGHT 25/09
0
0.05
0.1
0.15
0.2
0.25
0.3
0 5 10 15 20 25 30time (min)
CO
2 (%
)
Sph 1 Sph 2 Sph 3 Erio 1 Erio 2 Erio 3
- 22 cm
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1st succession peat – CH4
DARK 25/09
0.01.02.03.04.05.06.07.08.09.0
10.0
0 5 10 15 20 25 30
time (min)
CH
4 in
pp
m
Sphagnum 1 Sphagnum 2 Sphagnum 3Eriophorum 1 Eriophorum 2 Eriophorum 3
LIGHT 25/09
0.01.02.03.04.05.06.07.08.09.0
10.0
0 5 10 15 20 25 30
time (min)
CH
4 i
n p
pm
Sphagnum 1 Sphagnum 2 Sphagnum 3
Eriophorum 1 Eriophorum 2 Eriophorum 3
- 22 cm - 22 cm
DARK 16/10
0.00.51.01.52.02.53.03.54.04.55.0
0 5 10 15 20 25 30time (min)
CH
4 i
n p
pm
Sphagnum 1 Sphagnum 2 Sphagnum 3
Eriophorum 1 Eriophorum 2 Eriophorum 3
- 7 cm
LIGHT 16/10
0.00.51.01.52.02.53.03.54.04.55.0
0 5 10 15 20 25 30time (min)
CH
4 in
pp
m
Sphagnum 1 Sphagnum 2 Sphagnum 3
Eriophorum 1 Eriophorum 2 Eriophorum 3
- 7 cm
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Regenerating peat – CO2
DARK 30/09
0
0.005
0.01
0.015
0.02
0.025
0.03
0.035
0.04
0 5 10 15 20 25 30
time (min)
CO
2 (%
)
Sph 1 Sph 2 Sph 3 Poly1 Poly2 Poly3 LIGHT 30/09
-0.2
-0.15
-0.1
-0.05
0
0.05
0 5 10 15 20 25 30
time (min)
CO
2 (%
)
Sph 1 Sph 2 Sph 3 Poly1 Poly2 Poly3
- 38 cm - 38 cm
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Regenerating peat – CH4
DARK 30/09
0.0
0.5
1.0
1.5
2.0
2.5
3.0
3.5
4.0
4.5
5.0
0 5 10 15 20 25 30time (min)
CH
4 in
pp
m
Sphagnum 1 Sphagnum 2 Sphagnum 3
Polytrichum 1 Polytrichum 2 Polytrichum 3
LIGHT 30/09
0.0
0.5
1.0
1.5
2.0
2.5
3.0
3.5
4.0
4.5
5.0
0 10 20 30
time (min)C
H4
in p
pm
Sphagnum 1 Sphagnum 2 Sphagnum 3
Polytrichum 1 Polytrichum 2 Polytrichum 3
- 38 cm - 38 cm
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III. Fungal community structure analysis
by DGGE of ribosomal 18S and/or ITS sequences
Preliminary data
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Test DNA extractions for shipping methods
0
10
20
30
40
50
60
Living sphagnumlayer
Low humificationpeat
Medianhumification peat
High humificationpeat
Milled surfacepeat
mg
ml-1
DN
A
NO TREATMENT LIQUID N2 FREEZE-DRIED
***
*p < 0.05
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M + NT/S LN/S FD/S NT/P1 LN/P1 FD/P1 Sphagnum Low humification
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+ M NT/P2 LN/P2 FD/P2 NT/E LN/E FD/E + M
Medium humification High humification
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M + P2old P2new NT/M LN/M FD/M + + M
PCR FD/P2 Milled surface
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M + NT LN FD Regenerating S E
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Fungal DGGE analysis shows significant differences in band patterns according to peat depth
but: GELS CANNOT BE INTERCOMPARED (YET)
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IV. Community level physiological profiling
by MicroResp
Preliminary data
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• ‘Standard’ method utilises sieved soil of ca. 40% WHCproblematic with peat due to irreversible drying and fibrosity
• Protocol adapted to use slurry (peat blended with water 1:10)
• tested with glucose (150 and 75 mM)
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0.0
0.1
0.2
0.3
0.4
0.5
0.6
0 200 400 600 800 1000 1200 1400
time (min)
A 5
90
nm
20-30 bare 10-20 bare 5-10 bare 0-5 bare
20-30 >5 10-20 >5 5-10 >5 0-5 >5
20-30 >50 10-20 > 50 5-10 >50 control
Blank
only the surface sample at Regenerating peat site gives significantly higher SIR than a water blank (not sterile). No distinction possible between samples so far.
150 mM glucose, 2.5 mM bicarb
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2.5 mM
0
0.1
0.2
0.3
0.4
0.5
0.6
0.7
0 5 10 15 20 25time (h)
A 5
90
nm
2.0 mM0.0
0.1
0.2
0.3
0.4
0.5
0.6
0.7
0 5 10 15 20 25
time (h)
A 5
90
nm
1.5 mM0.0
0.1
0.2
0.3
0.4
0.5
0.6
0.7
0 5 10 15 20 25time (h)
A 5
90 n
m
1.0 mM0.0
0.1
0.2
0.3
0.4
0.5
0.6
0.7
0 5 10 15 20 25time (h)
A 5
90
nm
75 mM glucose, varying bicarb
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0.5 mM
0.10
0.15
0.20
0.25
0.30
0.35
0.40
0.45
0.50
0 5 10 15 20 25
time (h)
A 5
90 n
m
All 4 peat depths at Regenerating site, 0-5 cm horizon at 1st succession site, and all except the 0-5 cm horizon at the bare site show significantly higher CO2 evolution than the (water only) control. Different samples show different CO2 curves.
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Lowering the buffering capacity of the detection system allows for sample distinction.
Further work:
incorporation of different carbon sources to allow for CLPP using 32 carbon sources per plate (n = 3) or 16 carbon sources (n = 6).
Anoxic system?
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V. RECIPE WEBSITE
Preliminary version available at
http://www.macaulay.ac.uk/recipe
Will go public soon …