Reagents for histological techniques, haematology and...

Reagents for histological techniques, haematology and microbiology

Panreac has a full range of products for histology, haematology and microbiology, which includes the most commonly used reagents in the process of preparing samples for examination under the microscope. With this range, all the stages of fixing, rinsing, paraffin inclusion, staining and mounting are covered.

The majority of the products used in this technique are encompassed in the DC, or Clinical Diagnosis, quality, with the CE mark in compliance with the provisions of the European Directive on products for “in vitro” diagnosis.

Fixing: The range includes the majority of reagents used for this purpose, starting with the classic buffered formaldehyde, in a wide range of packaging formats, used in the normal way. Other fixing agents are also available for specific applications, such as Carnoy, Zenker or Bouin Fixing Agents, all of which are manufactured with the most widely accepted formulas.

We have recently included a formaldehyde surrogate, Histofix®, which substitutes for formaldehyde in its applications as a fixing agent, and is much less toxic, making it easier to handle and use.

As manufacturers, we are in a position to custom-produce fixing reagents with specific formulations for special applications.

Drying and Rinsing: The range of solvents for the drying stage includes different types of alcohols, chiefly ethanol, in different, ready-to-use concentrations, including a specific drying agent for pathological anatomy, VITROSEC® , and other solvents used for this purpose. All are specially purified and subjected to stringent quality control, to prevent the presence of particles that could cause artefacts in the preparation of tissues.

Reagents for histological techniques,

haematology and microbiology

The range of rinsing agents includes the classics: Xylene, Citrosol, and other solvents, together

with the new Isoparaffin H, a Xylene surrogate, non-aromatic, practically odourless, and

much less toxic than Xylene.

Inclusion: Paraffin inclusion is widely covered with paraffins with different melting

points, including the popular Paraplast®. This complete range of paraffins meets the

vast majority of needs in this field.

Staining: The most commonly used stains are available in ready-to-use solutions,

complemented by over 60 powdered dyes for preparation of any kind of stain with maximum

flexibility.

All the dyes are subjected to stringent quality control, to ensure maximum reproducibility in the preparation of the stains,

preventing colour differences between different preparations. This is particularly important for prepared solutions, where the

concentrations of dye or pH are specifically controlled, in order to ensure maximum contrast and homogeneous colour brightness

lot after lot, with special emphasis on the different available formulations of hematoxylin-eosin in Papanicolau dyes for

cytological studies.

The range includes different kits: Gram staining in microbiology, fast staining in haematology, and the kit for staining Reticulin

fibres.

Mounting: A wide range is available, from classic mounting media, Canada balsam and DPX, to the latest Mounting Medium

for Xylene Surrogates, indicated for use in conjunction with Isoparaffin H. All of them with one purpose: To ensure perfect

preservation of the preparations over time.

All in all, Panreac offers you a complete range of products for pathological anatomy, with the maximum guarantee to ensure

safe, reliable results in the histo-pathological process, thanks to the top quality and stringency of products designed specifically for

this purpose.

fixing

drying

inclusion

cut

staining

Samples of preparations

Sample: Lung cartilague. Method: Toluidine blue. Dr. Miquel Borràs - Unidad de Toxicología Experimental y Ecotoxicología. Parc Científic de Barcelona.

Sample: Neuronal weave. Method: Hematoxylin-Eosin. Dr. Melchor Saiz-Pardo and Dr. Julián Sanz Esponera. Hospital Clínico Universitario San Carlos. Servicio de Anatomía Patológica. Madrid.

Sample: Liver. Method: Hematoxylin-Eosin. Dr. Miquel Borràs - Unidad de Toxicología Experimental y Ecotoxicología. Parc Científic de Barcelona.

Sample: Peripheral blood swab. Method: Fast Kit (Panoptic). Dr. Verísimo Marta. Clinical Analysis Service, Grande Covian Medicine and Speciality Centre. Zaragoza.

Sample: Peripheral blood swab. Method: Fast Kit (Panoptic). Dr. Verísimo Marta. Clinical Analysis Service, Grande Covian Medicine and Speciality Centre. Zaragoza.

Sample: Large intestine. Method: Alcian Blue. Dr. Francesc Tresserra. Clínica Dexeus. Servicio Anatomía Patológica. Barcelona.

Sample: Liver. Method: Reticulin. Dr. Francesc Tresserra. Clínica Dexeus. Servicio Anatomía Patológica. Barcelona.

Sample: Skin. Method: Hematoxylin-Eosin. Rinsing with Isoparafin H (Xylene substitute). Dr. Francesc Tresserra. Clínica Dexeus. Servicio Anatomía Patológica. Barcelona.

Sample: GN Diffuse. Method: Masson’s Trichromic.Dr. Orradre Romeo. Complejo Hospitalario Virgen de la Salud. Servicio de Anatomía Patológica. Toledo.

Sample: Basal cells. Method: Papanicolau. Dr. J.C. Morín. - Cytology Centre. Las Palmas G.C.

Sample: Endometrium. Method: Papanicolau. Dr. J.C. Morín. - Cytology Centre. Las Palmas G.C.

Sample: Cervico-vaginal cytology. Method: Papanicolaou. Sta. María del Rosell Hospital. Pathological Anatomy Service, Cartagena.

Samples of preparations

Sample: Liver. Method: Reticulin. Dr. Juan J. Jimenez and Dra. Isabel Hierro. Antequera Hospital. Málaga.

Sample: Skin. Method: Masson’s Trichromic. Dr. Juan J. Jimenez and Dra. Isabel Hierro. Antequera Hospital. Málaga.

Sample: Stomach. Method: PAS-Alcian Blue. Dr. Juan J. Jimenez and Dr. Isabel Hierro. Antequera Hospital. Malaga.

Sample: Small intestine. Method: Reticulin. “Ciudad de Jaén” General Speciality Hospital. Pathological Anatomy Service. Jaén.

Sample: Liver. Method: Perls’ Blue. “Ciudad de Jaén” General Speciality Hospital. Pathological Anatomy Service. Jaén.

Sample: Small intestine. Method: PAS-Alcian Blue. “Ciudad de Jaén” General Speciality Hospital. Pathological Anatomy Service. Jaén.

Sample Lung. Method: Ziehl-Neelsen. Dr. Mª Ángeles López and Dr. David Marcilla. Virgen del Rocío University Hospital. Sevilla.

Sample: Small intestine. Method: Orcein. “Ciudad de Jaén” General Speciality Hospital. Pathological Anatomy Service. Jaén.

Sample: Skin. Method: May Grünwald-Giemsa. Dr. Mª Ángeles López and Dr. David Marcilla. Virgen del Rocío University Hospital. Sevilla.

Sample: Soft parts. Method: Congo Red. Dr. Mª Ángeles López and Dr. David Marcilla. Virgen del Rocío University Hospital. Sevilla.

Sample: Skin. Method: Verhoeff. Dr. Mª Ángeles López and Dr. David Marcilla. Virgen del Rocío University Hospital. Sevilla.

Sample: Intestinal gland. Method: Hematoxylin-Eosin.Dr. Carbajo. Ciudad Real Hospital Complex.

Fixing Agents Ethanol absolute DC 251086

Ethanol 96% v/v DC 251085

Ethanol-Diethyl Ether 1:1 DC 251084

Fixing B-5 DC 253500

Composition:Mercury (II) Chloride .....................................................6 gSodium Acetate 3-hydrate .........................................2,06 gWater s.q.m. .............................................................100 ml

Carnoy’s Fixing DC 251917

Composition:Acetic Acid glacial.....................................................100 mlEthanol absolute .......................................................600 mlTrichloromethane est. with ethanol ..............................300 ml

Zenker’s Fixing DC 254990

Composition:Mercury (II) Chloride .....................................................5 gPotassium Dichromate ................................................2,5 gSodium Sulphate anhydrous ...........................................1 gWater s.q.m. .............................................................100 ml

Formaldehyde 30-36% w/v concentrated buffered to pH=7 stabilized with methanol DC 253572

Formaldehyde 3,7-4,0% buffered to pH=7 stabilized with methanol DC 252931

Histofix® Substitute of Formaldehyde DC 255805

(® Registered trade-mark of Panreac Química S.A.U.)Composition:Glyoxal ................................................................ 15-25 %Ethanol absolute ....................................................... 5-8 %Acetic Acid glacial......................................................<5 %Methanol ..............................................................< 0,5 %

Bouin Liquor DC 254102

Composition:Picric Acid moistened with ~33% of H2O .................1,125 gAcetic Acid glacial.........................................................5 mlFormaldehyde 35-40% ................................................25 mlWater .........................................................................77 ml

Fixing reagents for electronic microscopy

Glutaraldehyde solution 25% DC 253857

Osmium(VIII) Oxide PA-ACS 132901

Osmium(VIII) Oxide solution 4% DC 255793

Composition:Osmium (VIII) Oxide ...................................................40 gWater s.q.m. ...........................................................1000 ml

Fixative Reagent DC 255281

bufferComposition:Sodium Cacodylate ..................................................2,14 gHydrochloric Acid1 mol/l ...............................................4 mlCalcium Chloride 0,1 mol/l ...........................................1 mlSaccharose...................................................................3 gWater s.q.m. .............................................................100 mlpH: ...........................................................................7,4

Drying and rinsing agents Ethanol 70% v/v (BP) CODEX 192695


Ethanol absolute DC 251086

Vitrosec® 70 dehydrating solvent for pathological anatomy DC 256067(® Registered trade-mark of Panreac Química S.A.U.)Composition:Ethanol absolute denatured with bitrex (v/v) ..............68,75 %Methanol (v/v) .........................................................1,25 %Water (v/v) .................................................................30 %

Vitrosec® 80 dehydrating solvent for pathological anatomy DC 256068(® Registered trade-mark of Panreac Química S.A.U.)Composition:Ethanol absolute denatured with bitrex (v/v) ..............78,75 %Methanol (v/v) .........................................................1,25 %Water (v/v) .................................................................20 %

Vitrosec® 96 dehydrating solvent for pathological anatomy DC 256066

(® Registered trade-mark of Panreac Química S.A.U.)Composition:Ethanol 96% partially denatured with bitrex (v/v) .......98,75 %Methanol (v/v) .........................................................1,25 %

Vitrosec® 100 dehydrating solvent for pathological anatomy DC 256065

(® Registered trade-mark of Panreac Química S.A.U.)Composition:Ethanol absolute denatured with bitrex (v/v) ..............98,75 %Methanol (v/v) .........................................................1,25 %

Xilene, mixture of isomers DC 251769

Citrosol (Xylene substitute) DC 253139

Isoparafine H (Xylene substitute) DC 255069

Toluene PRS 141745

Decalcifiers Histofix® Decalcifier 1 DC 256239

(® Registered trade-mark of Panreac Química S.A.U.) slow decalcifier and fixing agent

Histofix® marrow decalcifier DC 256284

(® Registered trade-mark of Panreac Química S.A.U.)bone marrow decalcification and fixation

Histofix® Decalcifier 2 DC 256238

(® Registered trade-mark of Panreac Química S.A.U.) medium decalcifier for fixed tissues

Histofix® Decalcifier 3 DC 256237

(® Registered trade-mark of Panreac Química S.A.U.) fast decalcifier for fixed tissues

Inclusion paraffinsParaffin M.P. 51-53°C pellets DC 253209

Paraffin M.P. 52ºC plasticized pellets DC 255803

Paraffin M.P. 56-58°C pellets DC 253211

Paraffin M.P. 56-58°C plasticized pellets DC 252913

Paraffin M.P. 56-58°C plasticized + DMSO pellets DC 254667

Paraplast X-Tra®, Paraffin M.P. 52°C pellets PPXTRA

(® Registered trade-mark of McCormick Scientific, LLC.)

Paraplast Plus®, Paraffin M.P. 56°C + DMSOpellets PPPLUS


Paraplast®, Paraffin M.P. 56-58°C pellets PPLAST


Powdered dyesAcridine Orange (C.I. 46005) DC 252321

fluorescent staining

Alcian Blue 8 GX (C.I. 74240) DC 254584

Certified by Biological Stain Commissionfor AMP (Acid mucopolysaccharides)

Amido Black 10B (C.I. 20470) DC 252036

for electrophoresis

Aniline Blue WS (C.I. 42755) DC 253708

collagen staining

Auramine O (C.I. 41000) DC 251162


Azur B (C.I. 52010) DC 255075

Azur C (C.I. 52002) DC 252419

blood smears staining

Azur-Eosin-Methylene Blue dye according to Giemsa DC 251337

blood smears and protozoos staining

Azur II (C.I. 52010 + 52015) DC 251178


Biebrich Scarlet (C.I. 26905) DC 253986

cytoplasm staining

Bismarck Brown R (C.I. 21010) DC 253934

fat staining

Bismarck Brown Y (C.I. 21000) DC 253935

mucin and cartilage staining

Brilliant Blue FCF (C.I. 42090) DC 254367

Brilliant Cresyl Blue (C.I. 51010) DC 251169

platelets and thrombocytes staining

Brilliant Green (C.I. 42040) DC 251758

vegetal tissue staining

Bromophenol Blue DC 251165

proteins staining

Bromothymol Blue DC 251167

vital staining

Carmine (Lacquer of carminic acidwith calcium and aluminium) (C.I. 75470) DC 251824

nucleus and glycogen staining

Carminic Acid (C.I. 75470) DC 254354

Congo Red (C.I. 22120) DC 251611

bacteriology

Coomassie Brilliant Blue G 250 (C.I. 42655) DC 254933

Coomassie Brilliant Blue R 250 (C.I. 42660) DC 254932

Crystal Violet (C.I. 42555) DC 251762

bacteria staining

Eosin Bluish (C.I. 45400) DC 252782

vital staining and plasma staining

Eosin-Methylene Blue dyeaccording to Leishman DC 251377


Eosin-Methylene Blue dyeaccording to May Grünwald DC 251415


Eosin-Methylene Blue dyeaccording to Wright DC 251767


Eosin Yellowish (C.I. 45380) DC 251299

vital staining and plasma staining

Erythrosin B (C.I. 45430) DC 253982

Evans Blue (C.I. 23860) DC 255486

Fast Green FCF (C.I. 42053) DC 255668

collagen staining

Fuchsin Acid (C.I. 42685) DC 251331


Fuchsin Basic (C.I. 42510) DC 251332

nucleus and Koch´s bacilli staining

Pararosaniline base (C.I. 42500) DC 254615

Phloxine B (C.I. 45410) DC 252081

bacteria staining, protozoos and seaweed, recount of eosinophils

Ponceau S (C.I. 27195) DC 253983

for electrophoresis

Resazurin DC 251591

sterility test

Rhodamine B (C.I. 45170) DC 251604


Rose Bengal (C.I. 45440) DC 253893

Safranine O (C.I. 50240) DC 251622

nucleus staining according to Gram

Sudan III (C.I. 26100) DC 251731

fatty acids and neutral fats staining in faeces

Sudan IV (C.I. 26105) DC 251858

fatty acids and neutral fats staining in faeces

Sudan Black B (C.I. 26150) DC 252069

fat staining

Tartrazine (C.I. 19140) DC 251734

celular body inclusion

Thionin (C.I. 52000) DC 251742

nucleus and mucosae staining

Toluidine Blue O (C.I. 52040) DC 251176

nucleus and mucosae staining

Victoria Blue B (C.I. 44045) DC 251177

bacteriology

Powdered dyes (cont.)Gentian Violet (C.I. 42535+42555) DC 251765

bacteria staining according to Gram

Hematoxylin (C.I. 75290) DC 251344

nucleus staining

Indigo Carmine (C.I. 73015) DC 251246

nucleus and glycogen staining

Lissamine Green B (C.I. 44090) DC 253987

plasma staining

Malachite Oxalate Green (C.I. 42000) DC 251761

cytoplasm of vegetal cells staining

Methyl Green (C.I. 42585) DC 251704

bacteria staining

Methyl Violet (C.I. 42535) DC 252079

diphterial bacteria staining

Methylene Blue (C.I. 52015) DC 251170

bacteriology and cytology

Neutral Red (C.I. 50040) DC 251619

Koch’s bacilli staining

Nigrosine water soluble (C.I. 50420) DC 254419

Nile Blue A Chloride (C.I. 51180) DC 254968

lipids staining

Orange II (C.I. 15510) DC 251814

Orange G (C.I. 16230) PA 123596

cytoplasm staining

Orcein DC 251324

chromosome staining

Dyes in solution Lactophenol Blue solution DC 253724

bacteriologyComposition:Methyl Blue ................................................................50 mgPhenol .......................................................................25 gL(+)-Lactic Acid .......................................................20,8 mlGlycerol ..................................................................39,5 mlWater s.q.m. .............................................................100 ml

Methylene Blue Alkali DC 251171

solution according to LoefflerComposition:Methylene Blue ......................................................0,365 gEthanol absolute ........................................................9,1 mlPotassium Hydroxide 0,1 mol/l ..................................1,62 mlWater .........................................................................91 ml

Methylene Blue Phenicated DC 251172

bacteria staining according to Ziehl-Neelsen, contrast dyeComposition:Methylene Blue .............................................................9 gEthanol absolute .........................................................90 mlPhenol .......................................................................26 mlWater s.q.m. ...........................................................1000 ml

Toluidine Blue O solution 1% DC 253535

nucleus and mucosae stainingComposition:Toluidine Blue O ...........................................................1 gWater s.q.m. .............................................................100 ml

Blue for fast staining (Panoptic nº 3) DC 253998

blood smear staining or medullary smear stainingComposition:Azur B ..........................................................................2 gBuffer solution pH 7 s.q.m. ......................................1000 ml

Dyes in solution (cont.)

Eosines

Eosin Yellowish hydroalcoholic solution 1% DC 251301

cytoplasm stainingComposition:Eosin Yellowish .............................................................1 gWater .........................................................................90 mlEthanol absolute .........................................................10 ml

Azur-Eosin-Methylene Blue solution according to Giemsa (slow) DC 251338

blood smears and protozoos stainingComposition:Azur-Eosin-Methylene Blue dye according to Giemsa .......0,5 gMethanol ...................................................................50 mlGlycerol .....................................................................50 ml

Eosin-Methylene Blue solution according to Leishman DC 251378

blood smears stainingComposition:Eosin-Methylene Blue dye according to Leishman ........0,25 gMethanol s.q.m. .......................................................100 ml

Eosin-Methylene Blue solution according to May Grünwald DC 251416

blood smears stainingComposition:Eosin-Methylene Blue dye according to May Grünwald .....................................................0,25 gMethanol s.q.m. .......................................................100 ml

Eosin-Methylene Blue solution according to Wright DC 251768

blood smears stainingComposition:Eosin-Methylene Blue dye according to Wright ............0,25 gMethanol s.q.m. .......................................................100 ml

Eosin for fast staining (Panoptic nº2) DC 253999

Composition:Eosin Yellowish ..........................................................0,8 gBuffer solution pH 7 s.q.m. ......................................1000 ml

Fixing for fast staining (Panoptic nº1) DC 254101

Composition:Crystal Violet ................................................................2 mgMethanol s.q.m. .....................................................1000 ml

Fuchsin Basic-Carbol solution according to Ziehl DC 251333

bacteria staining according to Gram-Nicolle and Ziehl-Neelsen, contrast dyeComposition:Basic Fuchsin ...........................................................0,74 gEthanol absolute .........................................................10 mlPhenol .........................................................................5 mlWater s.q.m. .............................................................100 ml

Hematoxylines

Carazzi’s Hematoxylin solution DC 255298

nucleus stainingComposition:Aluminium Potassium Sulphate 12-hydrate ......................5 gSodium Iodate .........................................................0,02 gHematoxylin ..............................................................0,1 gGlycerol .....................................................................20 mlWater s.q.m. .............................................................100 ml

Gill’s Hematoxylin I solution DC 252974

nucleus stainingComposition:Aluminium Sulphate 18-hydrate ................................1,76 gSodium Iodate ............................................................20 mgHematoxylin .............................................................200 mgAcetic Acid glacial.........................................................2 mlEthylene Glycol ...........................................................25 mlWater .........................................................................73 ml

Gill’s Hematoxylin II solution DC 252998

nucleus stainingComposition:Aluminium Sulphate 18-hydrate ................................3,52 gSodium Iodate ............................................................40 mgHematoxylin .............................................................400 mgAcetic Acid glacial......................................................3,8 mlEthylene Glycol ...........................................................25 mlWater .........................................................................71 ml

Gill’s Hematoxylin III solution DC 252999

nucleus stainingComposition:Aluminium Sulphate 18-hydrate ................................5,28 gSodium Iodate ............................................................60 mgHematoxylin .............................................................600 mgAcetic Acid glacial......................................................5,7 mlEthylene Glycol ...........................................................25 mlWater .........................................................................69 ml

Harris Hematoxylin solution DC 253949

nucleus stainingComposition:Aluminium Potassium Sulphate 12-hydrate ....................10 gMercury(II) Oxide yellow ............................................250 mgHematoxylin .............................................................500 mgEthanol 96% ...............................................................16 mlWater .........................................................................88 ml

Hematoxylin solution A according to Weigert DC 253453

nucleus stainingComposition:Hematoxylin .................................................................1 gEthanol absolute .......................................................100 ml

Hematoxylin solution B according to Weigert DC 253454

nucleus stainingComposition:Iron(III) Chloride 30% aqueous solution ..........................4 mlHydrochloric Acid 35% ..................................................1 mlWater s.q.m. .............................................................100 ml

Mayer’s Hematoxylin solution DC 254766

nucleus stainingComposition:Aluminium Potassium Sulphate 12-hydrate ....................50 gSodium Iodate ...........................................................0,2 gHematoxylin ..............................................................1,0 gCitric Acid anhydrous ....................................................1 gChloral hydrate ...........................................................50 gWater .....................................................................1000 ml

Dyes in solution (cont.) Other Dyes

Brilliant Green aqueous solution 5% DC 252339

vegetal tissue stainingComposition:Brilliant Green ..............................................................5 gWater s.q.m. .............................................................100 ml

Crystal Violet Oxalate solution according to Gram-Hucker DC 252532

bacteria staining according to Gram-HuckerComposition:Crystal Violet ..............................................................20 gAmmonium Oxalate ......................................................8 gEthanol ....................................................................200 mlWater .......................................................................800 ml

Crystal Violet solution 2% DC 251763

bacteria staining according to GramComposition:Crystal Violet ................................................................2 gMethanol s.q.m. .......................................................100 ml

Gentian Violet Phenique DC 251766

bacteria staining according to Gram-NicolleComposition:Gentian Violet .........................................................0,67 gEthanol absolute ......................................................11,7 mlPhenol ....................................................................2,05 mlWater .......................................................................100 ml

Light Green solution 0,1% DC 253524

animal tissue stainingComposition:Light Green SF yellowish .............................................0,1 gWater s.q.m. .............................................................100 ml

Methyl Red solution 0,1% DC 251618

Composition:Methyl Red ...................................................................1 gEthanol 70% ...........................................................1000 ml

Orcein solution A hydroacetic-hydrochloric solution DC 251993

chromosome stainingComposition:Orcein .....................................................................2,0 gHydrochloric Acid 1 mol/l ...........................................8,3 mlAcetic Acid ..............................................................45,8 mlWater ......................................................................45,8 ml

Orcein solution B hydroacetic solution DC 251994

chromosome stainingComposition:Orcein .....................................................................2,0 gAcetic Acid .................................................................55 mlWater .........................................................................55 ml

Papanicolaou’s Solution OG 6 DC 253892

Composition:Orange G .................................................................0,2 gPhosphotungstic Acid hydrate ....................................0,02 gWater ......................................................................11,5 mlEthanol absolute ......................................................88,5 ml

Papanicolaou’s Solution EA 50 DC 253594

Composition:Light Green SF yellowish ..............................................58 mgPhosphotungstic Acid hydrate ....................................0,17 gBismark Brown R .........................................................40 mgWater ...........................................................................7 mlMethanol ...................................................................93 mlEosin Yellowish ......................................................0,225 gAcetic Acid glacial......................................................0,1 g

Safranine O solution 0,2% DC 251623

bacteria staining according to GramComposción:Safranine O ..............................................................0,2 gWater s.q.m. .............................................................100 ml

Safranine O solution 1% DC 252533

bacteria staining according to GramComposition:Safranine O .................................................................1 gWater s.q.m. .............................................................100 ml

Safranine O solution according to Gram-Hucker DC 252531

bacteria staining according to Gram-HuckerComposition:Safranine O ............................................................0,25 gEthanol absolute .........................................................10 mlWater s.q.m. .............................................................100 ml

Schiff’s Reagent DC 251588

Composition:Pararosaniline ............................................................0,1 gSodium Sulphite solution 10% ......................................10 mlHydrochloric Acid 35% ..................................................3 mlWater .........................................................................50 ml

Van Gieson II solution DC 253625

Composition:Picric Acid moistened with ~33% of H2O ...................1,57 gHydrochloric Acid 35% .............................................0,26 mlFuchsin Acid ..............................................................0,1 gWater s.q.m. .............................................................100 ml

Most widely used stains Alcian Blue Staining-PAS carbohydrates differentiation Acetic Acid glacial DC 251008

Periodic Acid (Reag. USP, Ph. Eur.) PA-ACS 132320

Water PA-ACS 131074

Alcian Blue 8 GX (C.I. 74240) DC 254584



Hydrochloric Acid 1 mol/l (1N) SV 181021


Composition:Aluminium Potassium Sulphate 12-hydrate ....................10 gMercury(II) Oxide yellow ............................................250 mgHematoxylin .............................................................500 mgEthanol 96% ...............................................................16 mlWater .........................................................................88 ml

Sodium Disulphite PA-ACS 131698

Perls’ Blue Staining Iron determination Hydrochloric Acid 37% PA-ACS-ISO 131020

Water PA-ACS 131074

Potassium Hexacyanoferrate(II) solution 10% w/v RE 171507

Reticulin Fibres Staining

Reticulin Kit DC 255115.0922

Comprised of:Reticulin Kit Reagent AReticulin Kit Reagent BReticulin Kit Reagent CReticulin Kit Reagent DReticulin Kit Reagent EReticulin Kit Reagent FReticulin Kit Reagent GInstructions Sheet

Gram Stainingbacteria identification Gram-positive and Gram-negative Water PA-ACS 131074


Lugol’s Liquor DC 251774

Composition:Potassium Iodide ......................................................0,66 gIodine .......................................................................0,4 gWater s.q.m. .............................................................100 ml

Safranine O solution 0,2% DC 251623

Composition:Safranine O ..............................................................0,2 gWater s.q.m. .............................................................100 ml

Crystal Violet solution 2% DC 251763

Composition:Crystal Violet ................................................................2 gMethanol s.q.m. .......................................................100 ml

Gram-Hucker Staining

Kit for Staining Gram-Hucker 254884.0922

Differentiation of Gram-Positive and Gram-Negative Bacteria

Spare parts: Water PA-ACS 131074

Alcohol-Acetone 7:3 DC 251803

Safranine O solution according to Gram-Hucker DC 252531

Composition:Safranine O ............................................................0,25 gEthanol absolute .........................................................10 mlWater s.q.m. .............................................................100 ml

Crystal Violet Oxalate solution according to Gram-Hucker DC 252532

Composition:Crystal Violet ..............................................................20 gAmmonium Oxalate ......................................................8 gEthanol ....................................................................200 mlWater .......................................................................800 ml



Most widely used stains (cont.)

Gram-Nicolle Staining Differentiation of Gram-Positive and Gram-Negative Bacteria Water PA-ACS 131074

Alcohol-Acetone 7:3 DC 251803


Composition:Basic Fuchsin ...........................................................0,74 gEthanol absolute .........................................................10 mlPhenol .........................................................................5 mlWater s.q.m. .............................................................100 ml



Gentian Violet Phenique DC 251766

Composition:Gentian Violet .........................................................0,67 gEthanol absolute ......................................................11,7 mlPhenol ....................................................................2,05 mlWater .......................................................................100 ml

May Grünwald-Giemsa or Pappenheim Staining differentiation of blood cells Azur-Eosin-Methylene Blue solution according to Giemsa (slow) DC 251338

Composition:Azur-Eosin-Methylene Blue dye according to Giemsa .................................................0,5 gMethanol ...................................................................50 mlGlycerol .....................................................................50 ml

Eosin-Methylene Blue solution according to May Grünwald DC 251416

Composition:Eosin-Methylene Blue dye according to May Grünwald ....................................0,25 gMethanol s.q.m. .......................................................100 ml

Methanol (Reag. Ph. Eur.) PA-ACS-ISO 131091

Buffer Solution pH 7,2 DC 252164

Composition:Potassium di-Hydrogen Phosphate ................................40 mgdi-Sodium Hydrogen Phosphate 12-hydrate ................151 mgWater s.q.m. .............................................................100 ml

Papanicolaou Staining early detection of cervical or vaginal cancer Water PA-ACS 131074




Papanicolaou’s Solution EA 50 DC 253594

Composition:Light Green SF yellowish ..............................................58 mgPhosphotungstic Acid hydrate ....................................0,17 gBismark Brown R .........................................................40 mgWater ...........................................................................7 mlMethanol ...................................................................93 mlEosin Yellowish ......................................................0,225 gAcetic Acid glacial......................................................0,1 g

Papanicolaou’s Solution OG 6 DC 253892

Composition:Orange G .................................................................0,2 gPhosphotungstic Acid hydrate ....................................0,02 gWater ......................................................................11,5 mlEthanol absolute ......................................................88,5 ml

PAS Staining (Periodic Acid Schiff) carbohydrates detection Hydrochloric Acid 1 mol/l (1N) SV 181021

Periodic Acid (Reag. USP, Ph. Eur.) PA-ACS 132320

Water PA-ACS 131074





Sodium Disulphite PA-ACS 131698

Most widely used stains (cont.)

Fast Staining in Haematology (Panoptic)

Kit for Fast Staining in Haematology (Fast Panoptic) DC 254807.0922

blood or medullary smear staining

Spare parts:

Blue for fast staining (Panoptic nº 3) DC 253998

Composition:Azur B ..........................................................................2 gBuffer solution pH 7 s.q.m. ......................................1000 ml

Eosin for fast staining (Panoptic nº2) DC 253999

Composition:Eosin Yellowish ..........................................................0,8 gBuffer solution pH 7 s.q.m. ......................................1000 ml

Fixing for fast staining (Panoptic nº1) DC 254101

Composition:Crystal Violet ................................................................2 mgMethanol s.q.m. .....................................................1000 ml

Buffer Solution pH 7,2 DC 252164

for hematology, according to WeiseComposition:Potassium di-Hydrogen Phosphate .............................40 mgdi-Sodium Hydrogen Phosphate 12-hydrate .............151 mgWater s.q.m. ..........................................................100 ml

Congo Red alkaline Staining tissue stain for amyloid Water PA-ACS 131074




Congo Red (C.I. 22120) DC 251611

Sodium Chloride PA-ACS-ISO 131659

Sodium Hydroxide pellets PA-ACS-ISO 131687

Masson’s Trichromic Staining connective tissue staining Acetic Acid glacial (RFE, USP, BP, Ph. Eur.) PRS-CODEX 141008

Phosphomolybdic Acid x-hydrate PA-ACS 131031

Phosphotungstic Acid hydrate PA 121033

Water PA-ACS 131074

Aniline Blue WS (C.I. 42755) DC 253708

Biebrich Scarlet (C.I. 26905) DC 253986

Fuchsin Acid (C.I. 42685) DC 251331


Composition:Hematoxylin .................................................................1 gEthanol absolute .......................................................100 ml


Composition:Iron(III) Chloride 30% aqueous solution ..........................4 mlHydrochloric Acid 35% ..................................................1 mlWater s.q.m. .............................................................100 ml

Bouin Liquor DC 254102

Composition:Picric Acid moistened with ~33% de H2O ................1,125 gAcetic Acid glacial.........................................................5 mlFormaldehyde 35-40% ................................................25 mlWater .........................................................................77 ml

Van Gieson Staining connective tissue staining Water PA-ACS 131074


Composition:Hematoxylin .................................................................1 gEthanol absolute .......................................................100 ml


Composition:Iron(III) Chloride 30% aqueous solution ..........................4 mlHydrochloric Acid 35% ..................................................1 mlWater s.q.m. .............................................................100 ml


Composition:Fuchsin Acid ..............................................................0,1 gPicric Acid moistened with ~33% de H2O ..................1,57 gHydrochloric Acid 35% .............................................0,26 mlWater s.q.m. .............................................................100 ml

Most widely used stains (cont.) Verhoeff Staining for elastic tissue fibers elastic fibers of connective tissue staining Water PA-ACS 131074


Hematoxylin (C.I. 75290) DC 251344

Iron(III) Chloride 6-hydrate PRS 141358

Potassium Iodide PA-ACS-ISO 131542


Composition:Fuchsin Acid ..............................................................0,1 gPicric Acid moistened with ~33% de H2O ..................1,57 gHydrochloric Acid 35% .............................................0,26 mlWater s.q.m. .............................................................100 ml

Iodine resublimed pearls PA-ACS 131771

Ziehl-Neelsen Staining acid-fast bacilli staining Water PA-ACS 131074

Alcohol-Hydrochloric 8:2 DC 251804

Composition:Hydrochloric Acid 35% ................................................20 mlEthanol absolute .........................................................80 ml

Methylene Blue Phenicated DC 251172

Composition:Methylene Blue .............................................................9 gEthanol absolute .........................................................90 mlPhenol .......................................................................26 mlWater s.q.m. ...........................................................1000 ml


Composition:Basic Fuchsin ...........................................................0,74 gEthanol absolute .........................................................10 mlPhenol .........................................................................5 mlWater s.q.m. .............................................................100 ml

Mounting media Canada Balsam DC 251179

DPX, mounting medium slow DC 256155

DPX, mounting medium fast (toluene base) DC 255254

Eukitt®, mounting medium DC 253681

(® Registered trade-mark of O. Kindler GmbH)

Gelatine Gold DC 251336

Histofluid®, mounting medium DC 255598

(® Registered trade-mark of Paul Marienfeld GmbH&Co.KG)

Mounting medium for substitutes of xylene DC 255811

Immersion media Cedar Wood Oil DC 251001

Immersion Oil DC 251002

Immersion Oil purified DC 254561

Other reagents Acetic Acid glacial DC 251008

for determination of cholesterol and glucose

Picric Acid saturated solution DC 251049

for determination of alcohol and creatinine

Sulphuric Acid 1/3 mol/l (2/3N) DC 251063

deproteinizer, Folin and Wu´s method

Trichloroacetic Acid solution 20% w/v DC 252373

deproteinizer

Gum Benzoin tincture solution 1:10 DC 251193

according to Guillain-Laroche

Brij® 35 solution aqueous 30% w/v DC 252317

(® Registered trade-mark of ICI)surfactants for automatical analysis

Copper(II) Sulphate solution d.1,050 DC 252195

for hematology, blood density



Other reagents (cont.)



Collodion solution 4% w/v (USP) PRS-CODEX 141278

Creatinine DC 251283

for standards

Lactophenol DC 251837

bacteriologyComposition:Phenol ....................................................................22,7 gGlycerol ..................................................................35,8 mlL(+)-Lactic Acid .......................................................18,8 mlWater ......................................................................22,7 ml

Hayem’s Liquor DC 251389

for hematology, hematiesComposition:Sodium Sulphate 10-hydrate .....................................6,07 gSodium Chloride ......................................................0,74 gMercury (II) Chloride ................................................0,15 gWater s.q.m. .............................................................100 ml

Türck’s Liquor DC 251390

for hematology, leucocytesComposition:Acetic Acid glacial......................................................1,5 mlMethylene Blue ..........................................................1,3 mgWater s.q.m. .............................................................100 ml

Mercury(II) Nitrate 0,005 mol/l (0,01N) DC 252138

for determination of chloride, according to Schales and Schales

Benedict’s Reagent qualitative DC 251550

for determination of glucose in urineComposition:Sodium Carbonate anhydrous .................................153,6 gCitric Acid 1-hydrate ................................................70,9 gCopper(II) Sulphate 5-hydrate ...................................15,6 gWater s.q.m. .................................................................1 l

Benedict’s Reagent quantitative DC 251551

for determination of glucoseComposition:Copper(II) Sulphate 5-hydrate .................................17,39 gSodium Carbonate anhydrous ...................................73,9 gtri-Potassium Citrate 1-hydrate ................................201,1 gPotassium Thiocyanate ...........................................114,1 gWater s.q.m. .................................................................1 l

Biuret’s Reagent DC 251820

for determination of proteinsComposition:Copper(II) Sulphate 5-hydrate .....................................1,5 gSodium and Potassium Tartrate 4-hydrate .....................4,5 gPotassium Iodide ........................................................0,5 gSodium Hydroxide pellets ............................................0,8 gWater s.q.m. .............................................................100 ml

Esbach’s Reagent DC 251560

for determination of albuminComposition:Picric Acid ....................................................................1 gCitric Acid 1-hydrate .....................................................2 gEthanol absolute .........................................................20 mlWater s.q.m. .............................................................100 ml

Fehling’s Reagent A DC 251563

for determination of glucose in urine and reducing sugarsComposition:Copper(II) Sulphate 5-hydrate .................................48,30 gSulphuric Acid 96% .......................................................1 mlWater s.q.m. .................................................................1 l

Fehling’s Reagent B DC 251564

for determination of glucose in urine and reducing sugarsComposition:Sodium Hydroxide .......................................................90 gPotassium Sodium Tartrate 4-hydrate ..........................300 gWater s.q.m. .................................................................1 l

Fehling’s Reagent Composite DC 253203

for determination of glucose in urineComposition:Fehling’s Reagent A ...................................................500 mlFehling’s Reagent B ...................................................500 ml

Folin’s Reagent A cuprotartaric DC 251565

for determination of amylasemie, galactose and glucoseComposition:Sodium Carbonate 10-hydrate ..................................10,7 gTartaric Acid ............................................................0,75 gCopper(II) Sulphate 5-hydrate ...................................0,45 gWater s.q.m. .............................................................100 ml

Folin’s Reagent B phosphotungstic-molybdic DC 251566

for determination of amylasemie, galactose and glucoseComposition:Molybdic Acid 85% ......................................................7 gSodium Tungstate 2-hydrate ...........................................1 gSodium Hydroxide pellets ...............................................4 gOrto-Phosphoric Acid 85% ..........................................25 mlWater s.q.m. .............................................................100 ml

Folin-Ciocalteu’s Reagent DC 251567

phenol reagentComposition:Sodium Tungstate 2-hydrate .........................................10 gSodium Molybdate 2-hydrate ......................................2,5 gOrto-Phosphoric Acid 85% ............................................5 mlHydrochloric Acid 35% ................................................10 mlLithium Sulphate 1-hydrate ..........................................15 gWater s.q.m. .............................................................100 ml

Folin-Denis’ Reagent DC 251568

for determination of uric acidComposition:Sodium Tungstate 2-hydrate .........................................10 gOrto-Phosphoric Acid 85% ...........................................8 mlWater s.q.m. .............................................................100 ml

Meyer’s Reagent DC 251579

for hematology, blood (hemoglobin) in faeces, urine, duodenal liquid and gastric juiceComposition:Potassium Hydroxide 85% pellets ..................................20 gZinc metal ..................................................................15 gPhenolphthalein ............................................................2 gWater s.q.m. .............................................................100 ml

Other reagents (cont.)

Millon’s Reagent DC 251580

for determination of phenols, tyrosine and albuminoidsComposition:Mercury metal ............................................................23 gNitric Acid 60% .......................................................33,3 mlWater .........................................................................68 ml

Pandy’s Reagent DC 251585

for determination of globulins in cephalorachidial liquidsComposition:Phenol crystallized ......................................................6,6 gWater .......................................................................100 ml



Schlesinger’s Reagent DC 251589

for determination of urobilinComposition:Zinc Acetate 2-hydrate ................................................10 gWater ...........................................................................4 mlEthanol absolute .........................................................96 ml

Sodium Citrate solution 3,8% DC 251355

for hematology, settling rateComposition:tri-Sodium Citrate 2-hydrate ........................................3,8 gWater s.q.m. .............................................................100 ml

di-Sodium Oxalate 0,1 mol/l (0,1M) DC 251707

for hematology, prothrombin timeComposition:di-Sodium Oxalate ...................................................1,35 gWater s.q.m. .............................................................100 ml

o-Toluidine solution 6% DC 252311

for determination of glucose in bloodComposition:o-Toluidine stabilized .....................................................6 mlThiourea ..................................................................150 mgAcetic Acid glacial s.q.m. ...........................................100 ml

Voges Proskauer A Reagent DC 254833

for identification in microbiologyComposition:1-Naphthol ................................................................50 gEthanol 70% .............................................................980 ml

Voges Proskauer B Reagent DC 254832

for identification in microbiologyComposition:Creatine .......................................................................3 gPotassium Hydroxide 40% ........................................1000 ml

Kovacs’ Reagent DC 252908

for detection of indole in microbiologyComposition:4-(Dimethylamino) benzaldehyde .................................50 gHydrochloric Acid 37% ..............................................250 ml1-Butanol .................................................................750 ml

Bibliography“Laboratorio de Anatomía Patológica”, Raimundo García del Moral, 1ª Ed. McGraw-Hill - Interamericana de España, Madrid, 1993“Laboratory Methods in Histotechnology”, American Registry of Pathology, Armed Forces Institute of Pathology, Washington, D.C., 1992“Staining Procedures”, Clark G., 4th Ed. Williams and Wilkins , Baltimore , 1981“Manual Técnico de Análisis Clínicos”, E. Suárez Peregrin , 9ª Ed. Prieto, 1972 “Gradwohl’s clinical laboratory methods and diagnosis”, S. Frankel, S. Reitman, A. C. Sonnenwirth, 7th Ed. The C. V. Mosby Company 2, 1970 “Lynch’s Medical Laboratory Technology”, Stanley S. Raphael, 3rd Ed. W. B. Saunders Company I, 1976

Laboratory glassware cleaning agents

DERQUIM products have been specially made for cleaning the laboratory equipment. They can be used in the manual cleaning (series LM) or for washing using automatic machines (series LA).

The various formulations are specifically adapted to any kind of laboratory residue: chemical, biological or clinical.

Two products with disinfecting and bactericidal properties are included in our DERQUIM program. DERQUIM DSF 01 is used for hand disinfecting and DERQUIM DSF 11 is used for surfaces and instruments disinfecting.

For automatic or manual cleaningDERQUIM + Universal Detergent, LIQUID 503574

For manual cleaningDERQUIM LM 01 Alkaline LIQUID 502600

DERQUIM LM 02 Neutral, phosphates free LIQUID 502601

DERQUIM LM 03 Phosphates free LIQUID 502602

For automatic cleaningDERQUIM LA 11 Slightly Alkaline SOLID 502603

DERQUIM LA 12 Alkaline SOLID 502604

DERQUIM LA 13 Alkaline with detergents SOLID 502605

DERQUIM LA 14 Slightly Alkaline LIQUID 502606

DERQUIM LA 15 Alkaline LIQUID 502607

DERQUIM LA 21 Acid, with phosphoric acid LIQUID 502608

DERQUIM LA 22 Acid, with citric acid LIQUID 502609

Automatic cleaning aidsDERQUIM LA 31 Antifoaming 502610

DERQUIM LA 32 Rinsing 502611

DERQUIM SALT (Sodium Chloride lumps) 503468

DisinfectantsDERQUIM DSF 01 Antiseptic for hands LIQUID 504993

DERQUIM DSF 11 Antiseptic for surfaces and implements LIQUID 504994

Chromic MixtureDERQUIM MC Chromic Mixture 502612

DERQUIM OXY (Substitute of Chromic Mixture) SOLID 506071

Order InformationAcetic Acid glacial DC 251008.1611 1000 ml a

Acridine Orange (C.I. 46005) DC 252321.1605 10 g a

252321.1606 25 g a

Alcian Blue 8 GX (C.I. 74240) DC 254584.1604 5 g a

254584.1606 25 g a

Alcohol-Acetone 7:3 DC 251803.1209 250 ml b

251803.1210 500 ml b

251803.1211 1000 ml b

Alcohol-Hydrochloric 8:2 DC 251804.1210 500 ml b

Amido Black 10B (C.I. 20470) DC 252036.1606 25 g a

252036.1608 100 g a

Aniline Blue WS (C.I. 42755) DC 253708.1604 5 g a

253708.1606 25 g a

Auramine O (C.I. 41000) DC 251162.1607 50 g a

251162.1608 100 g a

Azur II (C.I. 52010 + 52015) DC 251178.1606 25 g a

Azur B (C.I. 52010) DC 255075.1606 25 g a

Azur C (C.I. 52002) DC 252419.1606 25 g a

Azur-Eosin-Methylene Blue dye 251337.1606 25 g a

according to Giemsa DC 251337.1608 100 g a

Azur-Eosin-Methylene Blue solution 251338.1608 100 ml a

according to Giemsa (slow) DC 251338.1610 500 ml a

251338.1611 1000 ml a

251338.1612 2,5 l a

Benedict’s Reagent qualitative DC 251550.1210 500 ml b

251550.1211 1000 ml b

Benedict’s Reagent quantitative DC 251551.1210 500 ml b

251551.1211 1000 ml b

Biebrich Scarlet (C.I. 26905) DC 253986.1605 10 g a

253986.1606 25 g a

Bismarck Brown R (C.I. 21010) DC 253934.1606 25 g a

253934.1608 100 g a

Bismarck Brown Y (C.I. 21000) DC 253935.1606 25 g a

Biuret’s Reagent DC 251820.1208 100 ml b

Blue for fast staining (Panoptic nº3) DC 253998.1210 500 ml b

253998.1212 2,5 l b

Bouin Liquor DC 254102.1611 1000 ml a

Brij® 35 aqueous solution 30% w/v DC 252317.1611 1000 ml a

Brilliant Blue FCF (C.I. 42090) DC 254367.1606 25 g a

Brilliant Cresyl Blue (C.I. 51010) DC 251169.1604 5 g a

251169.1606 25 g a

Brilliant Green (C.I. 42040) DC 251758.1606 25 g a

251758.1608 100 g a

Brilliant Green aqueous solution 5% DC 252339.1608 100 ml a

252339.1609 250 ml a

Bromophenol Blue DC 251165.1604 5 g a

251165.1606 25 g a

Bromothymol Blue DC 251167.1604 5 g a

251167.1606 25 g a

Buffer Solution pH 7,2 DC 252164.1210 500 ml b

252164.1211 1000 ml b

252164.1212 2,5 l b

Canada Balsam DC 251179.1606 25 ml a

251179.1608 100 ml a

251179.1609 250 ml a

Carazzi’s Hematoxylin solution DC 255298.1610 500 ml a

255298.1612 2,5 l a

Carmine (Lacquer of carminic acid with 251824.1605 10 g a

calcium and aluminium) (C.I. 75470) DC 251824.1606 25 g a

Carminic Acid (C.I. 75470) DC 254354.1604 5 g a

Carnoy’s Fixing DC 251917.1611 1000 ml a

Cedar Wood Oil DC 251001.1606 25 ml a

251001.1607 50 ml a

Citrosol (substitute of Xylene) DC 253139.1611 1000 ml a

253139.1612 2,5 l a

253139.1214 5 l i

Collodion solution 4% w/v (USP) PRS-CODEX 141278.1609 250 ml a

141278.1611 1000 ml a

Congo Red (C.I. 22120) DC 251611.1605 10 g a

251611.1606 25 g a

Coomassie Brilliant Blue G 250 254933.1606 25 g a

(C.I. 42655) DC 254933.1608 100 g a

Coomassie Brilliant Blue R 250 (C.I. 42660) DC 254932.1606 25 g a

Copper(II) Sulphate solution d.1,050 DC 252195.2711 1000 ml



Creatinine DC 251283.1605 10 g a

Crystal Violet (C.I. 42555) DC 251762.1606 25 g a

251762.1608 100 g a

Crystal Violet Oxalate solution according to 252532.1608 100 ml a

Gram-Hucker DC 252532.1609 250 ml a

252532.1611 1000 ml a

Crystal Violet solution 2% DC 251763.1608 100 ml a

DERQUIM + Universal Detergent, 503574.1211 1000 ml b

LIQUID 503574.1231 3 l i

503574.1214 5 l i

503574.1315 10 l C

DERQUIM DSF 01 Antiseptic for hands 504993.1211 1000 ml b

LIQUID 504993.1231 3 l i

DERQUIM DSF 11 Antiseptic for 504994.1211 1000 ml b

surfaces and implements LIQUID 504994.1231 3 l i

504994.1214 5 l i

504994.1315 10 l C

DERQUIM LA 11 Slightly alkaline 502603.1245 2 kg G

SOLID 502603.0415 10 kg G

502603.0416 25 kg G

DERQUIM LA 12 Alkaline SOLID 502604.1245 2 kg G

502604.0415 10 kg G

502604.0416 25 kg G

DERQUIM LA 13 Alkaline with detergents 502605.1245 2 kg G

SOLID 502605.0415 10 kg G

502605.0416 25 kg G

DERQUIM LA 14 Slightly alkaline 502606.1231 3 l i

LIQUID 502606.1214 5 l i

502606.1315 10 l C

502606.0716 25 l i

DERQUIM LA 15 Alkaline LIQUID 502607.1231 3 l i

502607.1214 5 l i

502607.1315 10 l C

502607.0716 25 l i

DERQUIM LA 21 Acid, 502608.1231 3 l i

with phosphoric acid LIQUID 502608.1214 5 l i

502608.1315 10 l C

502608.0716 25 l i

DERQUIM LA 22 Acid, 502609.1231 3 l i

with citric acid LIQUID 502609.1214 5 l i

502609.1315 10 l C

502609.0716 25 l i

DERQUIM LA 31 Antifoaming 502610.1231 3 l i

DERQUIM LA 32 Rinsing 502611.1231 3 l i

DERQUIM SALT (Sodium Chloride lumps) 503468.0415 10 kg G

503468.0416 25 kg G

DERQUIM LM 01 Alkaline LIQUID 502600.1231 3 l i

502600.1214 5 l i

502600.1315 10 l C

502600.0716 25 l i

DERQUIM LM 02 Neutral, phosphates 502601.1231 3 l i

free LIQUID 502601.1214 5 l i

502601.1315 10 l C

502601.0716 25 l i

DERQUIM LM 03 phosphates free LIQUID 502602.1231 3 l i

502602.1214 5 l i

502602.1315 10 l C

502602.0716 25 l i

DERQUIM MC Chromic mixture 502612.2211 1000 ml 2

DERQUIM OXY (substitute of Chromic mixture) SOLID

506071.2322 12 x 10 g v

DPX, mounting medium slow DC 256155.1608 100 ml a

256155.1610 500 ml a

DPX, mounting medium fast 255254.1608 100 ml a

(toluene base) DC 255254.1610 500 ml a

Eosin Bluish (C.I. 45400) DC 252782.1606 25 g a

252782.1608 100 g a

Eosin for fast staining 253999.1210 500 ml b

(Panoptic nº2) DC 253999.1212 2,5 l b

Eosin-Methylene Blue dye according to Leishman DC

251377.1606 25 g a

Eosin-Methylene Blue solution 251378.1610 500 ml a

according to Leishman DC 251378.1611 1000 ml a

Eosin-Methylene Blue dye 251415.1606 25 g a

according to May Grünwald DC 251415.1608 100 g a


according to May Grünwald DC 251416.1610 500 ml a

251416.1611 1000 ml a

251416.1612 2,5 l a

Eosin-Methylene Blue dye 251767.1606 25 g a

according to Wright DC 251767.1608 100 g a


according to Wright DC 251768.1610 500 ml a

251768.1611 1000 ml a

Eosin Yellowish (C.I. 45380) DC 251299.1606 25 g a

251299.1608 100 g a

Eosin Yellowish hydroalcoholic 251301.1608 100 ml a

solution 1% DC 251301.1609 250 ml a

251301.1611 1000 ml a

Erythrosin B (C.I. 45430) DC 253982.1606 25 g a

Esbach’s Reagent DC 251560.1610 500 ml a

Ethanol absolute DC 251086.1211 1000 ml b

251086.1212 2,5 l b

251086.1214 5 l i

251086.1315 10 l C

Ethanol 96% v/v DC 251085.1211 1000 ml b

251085.1212 2,5 l b

251085.1214 5 l i

251085.1315 10 l C

Ethanol 70% v/v (BP) CODEX 192695.1211 1000 ml b

192695.1212 2,5 l b

192695.1214 5 l i

192695.1315 10 l C

192695.0716 25 l i

Ethanol-Diethyl Ether 1:1 DC 251084.1610 500 ml a

Eukitt®, mounting medium DC 253681.0008 100 ml z

253681.0009 250 ml z

253681.0010 500 ml z

Evans Blue (C.I. 23860) DC 255486.1606 25 g a

Fast Green FCF (C.I. 42053) DC 255668.1606 25 g a

Fehling’s Reagent A DC 251563.1210 500 ml b

251563.1211 1000 ml b

Fehling’s Reagent B DC 251564.1210 500 ml b

251564.1211 1000 ml b

Fehling’s Reagent Composite DC 253203.1611 1000 ml a

Fixative Reagent DC 255281.1210 500 ml b

Fixing B-5 DC 253500.1211 1000 ml b

Fixing for fast staining 254101.1210 500 ml b

(Panoptic nº1) DC 254101.1212 2,5 l b

Folin’s Reagent A cuprotartaric DC 251565.1609 250 ml a

Folin’s Reagent B phosphotungstic-molybdic DC

251566.1609 250 ml a

Folin-Ciocalteu’s Reagent DC 251567.1609 250 ml a

Folin-Denis’ Reagent DC 251568.1609 250 ml a

Formaldehyde 3,7-4,0% buffered to 252931.0922 48x20 ml 1

pH=7 and stabilized with methanol DC 252931.1211 1000 ml b252931.1212 2,5 l b252931.1214 5 l i252931.1315 10 l C252931.0716 25 l i

252931.0718 60 l i

Formaldehyde 30-36% w/v concentrated 253572.1211 1000 ml b

buffered to pH=7 stabilized with 253572.1212 2,5 l b

methanol DC 253572.1214 5 l i

253572.0716 25 l i

Fuchsin Acid (C.I. 42685) DC 251331.1605 10 g a

251331.1607 50 g a

Fuchsin Basic (C.I. 42510) DC 251332.1606 25 g a

251332.1608 100 g a

Fuchsin Basic-Carbol solution 251333.1609 250 ml a

according to Ziehl DC 251333.1611 1000 ml a

251333.1612 2,5 l a

Gelatine Gold DC 251336.0910 500 g v

251336.0911 1000 g v

Gentian Violet 251765.1606 25 g a

(C.I. 42535+42555) DC 251765.1608 100 g a

251765.1609 250 g a

Gentian Violet Phenique DC 251766.1608 100 ml a

251766.1609 250 ml a

Gill’s Hematoxylin I solution DC 252974.1211 1000 ml b

Gill’s Hematoxylin II solution DC 252998.1211 1000 ml b

Gill’s Hematoxylin III solution DC 252999.1211 1000 ml b

Glutaraldehyde solution 25% DC 253857.1611 1000 ml a

Gum Benzoin tincture solution 1:10 DC 251193.1608 100 ml a

Harris Hematoxylin solution DC 253949.1610 500 ml a

253949.1611 1000 ml a

253949.1612 2,5 l a

Hayem’s Liquor DC 251389.1209 250 ml b

Hematoxylin (C.I. 75290) DC 251344.1604 5 g a

251344.1606 25 g a

Hematoxylin solution A according to 253453.1210 500 ml b

Weigert DC 253453.1211 1000 ml b

Hematoxylin solution B according to 253454.1210 500 ml b

Weigert DC 253454.1211 1000 ml b

Histofix® Substitute of Formaldehyde DC 255805.2711 1000 ml

255805.2714 5 l

Histofix® Decalcifier 1 DC 256239.1211 1000 ml b

256239.1214 5 l i


256238.1214 5 l i


Histofix® marrow decalcifier DC 256284.0922 pack b

Histofluid®, mounting medium DC 255598.0010 500 ml z

Immersion Oil DC 251002.1207 50 ml l

251002.1208 100 ml l

Immersion Oil purified DC 254561.1207 50 ml l

254561.1208 100 ml l

Indigo Carmine (C.I. 73015) DC 251246.1605 10 g a

251246.1607 50 g a

Isoparaffin H (Substitute of Xylene) DC 255069.2711 1000 ml

255069.2714 5 lKit for Fast Staining in Haematology (Fast Panoptic) DC

254807.0922 pack v

Kit for staining Gram-Hucker DC 254884.0922 pack v

Kovacs’ Reagent DC 252908.1608 100 ml a

Lactophenol DC 251837.1608 100 ml a

Lactophenol Blue solution DC 253724.1608 100 ml a

Light Green solution 0,1% DC 253524.1210 500 ml b

Lissamine Green B (C.I. 44090) DC 253987.1606 25 g a

Lugol’s Liquor DC 251774.1608 100 ml a251774.1609 250 ml a251774.1611 1000 ml a

Malachite Oxalate Green (C.I. 42000) DC 251761.1606 25 g a

251761.1608 100 g a

Mayer’s Hematoxylin solution DC 254766.1610 500 ml a

254766.1611 1000 ml a

Mercury(II) Nitrate 0,005 mol/l (0,01N) DC 252138.1611 1000 ml a

Methanol (Reag. Ph. Eur.) PA-ACS-ISO 131091.1211 1000 ml b131091.1611 1000 ml a131091.1212 2,5 l b131091.1612 2,5 l a131091.1214 5 l i

131091.0716 25 l i

Methyl Green (C.I. 42585) DC 251704.1604 5 g a

251704.1606 25 g a

Methyl Red solution 0,1% DC 251618.1208 100 ml b

Methyl Violet (C.I. 42535) DC 252079.1606 25 g a

252079.1608 100 g a

Methylene Blue (C.I. 52015) DC 251170.1606 25 g a

251170.1608 100 g a

251170.1609 250 g a

Methylene Blue Alkali DC 251171.1208 100 ml l

251171.1209 250 ml b

Methylene Blue Phenicated DC 251172.1208 100 ml l251172.1209 250 ml b251172.1211 1000 ml b

Meyer’s Reagent DC 251579.1608 100 ml a

Millon’s Reagent DC 251580.1608 100 ml a

Mounting medium for substitutes of xylene DC

255811.0008 100 ml a

Neutral Red (C.I. 50040) DC 251619.1605 10 g a

251619.1606 25 g a

Nigrosine water soluble (C.I. 50420) DC 254419.1606 25 g a

Nile Blue A Chloride (C.I. 51180) DC 254968.1606 25 g a

Orange G (C.I. 16230) PA 123596.1606 25 g a

Orange II (C.I. 15510) DC 251814.1606 25 g a

Orcein DC 251324.1604 5 g a

251324.1606 25 g a

Orcein solution A 251993.1208 100 ml l

hydroacetic-hydrochloric solution DC 251993.1209 250 ml l

Orcein solution B hydroacetic solution DC 251994.1208 100 ml l

251994.1209 250 ml l

Osmium(VIII) Oxide PA-ACS 132901.0201 0,1 g k

132901.0202 0,5 g k

132901.0203 1 g k

Osmium(VIII) Oxide solution 4% DC 255793.1604 5 ml a

Pandy’s Reagent DC 251585.1609 250 ml a

Papanicolaou’s Solution EA 50 DC 253594.1610 500 ml a

253594.1611 1000 ml a

253594.1612 2,5 l a

Papanicolaou’s Solution OG 6 DC 253892.1610 500 ml a

253892.1611 1000 ml a

253892.1612 2,5 l a

Paraffin M.P. 51-53°C pellets DC 253209.1211 1000 g b253209.0914 5 kg 1

253209.0416 25 kg g

Paraffin M.P. 52ºC plasticized pellets DC 255803.1211 1000 g b

255803.0914 5 kg G

Paraffin M.P. 56-58°C pellets DC 253211.1211 1000 g b

253211.0914 5 kg 1

253211.0416 25 kg g

Paraffin M.P. 56-58°C plasticized+ DMSO pellets DC

254667.1211 1000 g b

254667.0914 5 kg 1

Paraffin M.P. 56-58°C plasticized 252913.1211 1000 g bpellets DC 252913.0914 5 kg 1

252913.0416 25 kg g

Paraplast X-Tra®, Paraffin M.P. 52°C pellets

PPXTRA.0933 6 x 1 kg 1

Paraplast Plus®, Paraffin M.P. 56°C + DMSO pellets

PPPLUS.0933 6 x 1 kg 1

Paraplast®, Paraffin M.P. 56-58°C pellets

PPLAST.0939 4 x 5 kg v

Pararosaniline base (C.I. 42500) DC 254615.1605 10 g a

Phloxine B (C.I. 45410) DC 252081.1604 5 g a

252081.1606 25 g a

Picric Acid saturated solution DC 251049.1610 500 ml a

Ponceau S (C.I. 27195) DC 253983.1604 5 g a

253983.1606 25 g a

Resazurin DC 251591.1604 5 g a

Reticulin Kit DC 255115.0922 pack v

Rhodamine B (C.I. 45170) DC 251604.1606 25 g a

251604.1608 100 g a

Rose Bengal (C.I. 45440) DC 253893.1606 25 g a

253893.1608 100 g a

Safranine O (C.I. 50240) DC 251622.1605 10 g a

251622.1607 50 g a

Safranine O solution 0,2% DC 251623.1208 100 ml l

Safranine O solution 1% DC 252533.1208 100 ml l

Safranine O solution according to 252531.1208 100 ml l

Gram-Hucker DC 252531.1209 250 ml l

252531.1211 1000 ml b

Schiff’s Reagent DC 251588.1609 250 ml a

251588.1611 1000 ml a

Schlesinger’s Reagent DC 251589.1609 250 ml a

Sodium Citrate solution 3,8% DC 251355.1210 500 ml b

251355.1211 1000 ml b

di-Sodium Oxalate 0,1 mol/l (0,1M) DC 251707.1211 1000 ml b

Sudan III (C.I. 26100) DC 251731.1606 25 g a

251731.1608 100 g a

Sudan IV (C.I. 26105) DC 251858.1606 25 g a

Sudan Black B (C.I. 26150) DC 252069.1606 25 g a

252069.1608 100 g a

Sulphuric Acid 1/3 mol/l (2/3N) DC 251063.1210 500 ml b

Tartrazine (C.I. 19140) DC 251734.1606 25 g a

251734.1608 100 g a

Thionin (C.I. 52000) DC 251742.1604 5 g a

251742.1606 25 g a

Toluene PRS 141745.1611 1000 ml a

141745.1612 2,5 l a

141745.0314 5 l z

o-Toluidine solution 6% DC 252311.1611 1000 ml a

Toluidine Blue O (C.I. 52040) DC 251176.1604 5 g a

251176.1606 25 g a

Toluidine Blue O solution 1% DC 253535.1210 500 ml b

Trichloroacetic Acid solution 20% w/v DC 252373.1611 1000 ml a

Türck’s Liquor DC 251390.1609 250 ml a

Van Gieson II solution DC 253625.1610 500 ml a

Victoria Blue B (C.I. 44045) DC 251177.1605 10 g a

Vitrosec® 100 dehydrating solvent 256065.2711 1000 mlfor pathological anatomy DC 256065.2714 5 l

256065.1315 10 l C


256066.1315 10 l C


Vitrosec® 70 dehydrating solvent 256067.2711 1000 ml

for pathological anatomy DC 256067.2714 5 l

Voges Proskauer A Reagent DC 254833.1208 100 ml b

Voges Proskauer B Reagent DC 254832.1208 100 ml b

Xylene, mixture of isomers DC 251769.2711 1000 ml251769.2714 5 l

Zenker’s Fixing DC 254990.1210 500 ml b

Packaging Symbolsa Glass bottle b Polyethylene bottle i Polyethylene canister

2 Glass bottle coated with PE or PVC z Aluminium bottle G Polypropylene bucket with handleg Carton drum with inner polyethylene bag Coextrussion bottle Coextrussion canisterk Glass or plastic ampoule 1 Carton box with inner polyethylene bagC Polyethylene container with removable tapl Polyethylene bottle with dropperv Box

Panreac Química S.A.U.

C/ Garraf, 2 - Polígono Pla de la Bruguera E-08211 Castellar del Vallès (Barcelona) Spain Tel. (+34) 937 489 400 Fax (+34) 937 489 401

e-mail: [email protected]

SALES

Tel. Spain / International (+34) 902 438 439 Fax Spain (+34) 937 489 494Fax International (+34) 937 489 495 e-mail: Spain: [email protected] Portugal: [email protected] France: [email protected] Rest of the world: [email protected]

Panreac Chimie S.A.R.L.129, rue ServientTour Credit Lyonnais69326 Lyon Cedex 03France

Panreac Quimica Lda.Avenida 25 de Abril, 672Edificio Alvorada - 5º Esq.º2750-512 CascaisPortugal

Nova ChimicaVia G. Galilei, 4720090 Cinisello Balsamo (MI)ItalyTel. +39 02 66045392Fax +39 02 66045394e-mail: [email protected]

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Reagents for histological techniques, haematology and...

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