Quantitative Real-Time PCR - شرکت آریا هوشمند آزما Real-Time PCR Real-Time PCR...
Transcript of Quantitative Real-Time PCR - شرکت آریا هوشمند آزما Real-Time PCR Real-Time PCR...
Diagnostics Worldwide
Molecular DX
Quantitative Real-Time PCR
> Highly sensitive detection> For all major or relevant genotypes> Ready to use kit including internal control and quantification standards> Validated for major Real-Time cyclers> CE-marked
CMV
HBV
HCV
HIV-1
New
Quantitative Real-Time PCR Real-Time PCR Kits
Reagent Concept
> Extended linear range
> Up to 100 determinations
> 4 external quantitative standards calibrated against WHO
> Validated for the isolation with Viral NA Isolation kits
from HUMAN
> Compatibility with widly used Real-Time cyclers:
HumaCycler*, Strategene Mx3000p/Mx3005p,
LightCycler 2.0, LineGene 9600, RotorGene 3000/6000/Q
> CE marked
Viral Nucleic Acid Isolation Kits
Viral NA Isolation kits provide a rapid and simple method
for isolation of high pure RNA and DNA at the same time
in a single application from viruses from serum or plasma.
Offers high degree efficiency at optimized tests for
use in both research and diagnostic clinical laboratories.
Ordering Information
Products* Unit REF
HIV-1 Real-Time PCR Kit** 100 tests 87100
HCV Real-Time PCR Kit 100 tests 87110
HBV Real-Time PCR Kit 100 tests 87120
CMV Real-Time PCR Kit 100 tests 87030
Viral Nucleic Acid Isolation Kit 100 tests 87300
Viral DNA Isolation Kit 100 tests 87310
Viral RNA Isolation Kit 100 tests 87320
Specifications
HBV HCV HIV-1 CVM
Genotype detection Eight HBV genotypes Seven HCV genotypes Nine HIV-1 subtypes Relevant geno – and subtypes
Analytical sensitivity 11.8 IU/ml 9.5 IU/ml 56 IU/ml 103 IU/ml
Linear range 11.8 - 1x109 IU/ml 9.5 - 1x109 IU/ml 56 - 1x109 IU/ml 103 - 1x109 IU/ml
Technique Spin column
Sample ____________________ serum or plasma
Yield ____________ variable according to viral load
Purity _____________ A260 / A 280 ratio: 1.70-1.90
Duration ______________________ About 25 min
* available Q1/2015
** only for use with REF 87300
Quantitative Real-Time PCR Real-Time PCR Analyzer
Basic Features
> Open system
> Walk away capability
> Optimized for use with HUMAN Real-Time PCR Kits
> Continuous operation mode
> Up to 96 samples,
12 x 8 strips & 96 x 0.2 ml tubes
fit for most full skirted plate,
> CE-IVD marking
Technical Data
> Bottom detection
> Peltier cooler inside
> Heating cooling rate: 4.0°C/s
> Sample volume 5-100 µl
> Block temperature range: 4...105°C
> Multi-socket transformer, fit for RS232C,
USB & bluetooth
> Dimensions W x D x H 43 x 40 x 35 cm
> Net weight 28 kg
> 230/110 VAC 50/60 Hz
HUMAN´s Real-Time Cycler “HumaCycler” will provide
exceptionally heating/cooling rate, temperature accuracy,
uniformity and stability. HumaCycler has up to 96 samples
capacity and implements 96 wells dual-color scanning only
within 5.5 seconds. With four channels, the instrument
covers the most commonly used fluorescence detection
wavelength.
Ordering Information REF
HumaCycler 86100
HumaCyclerReal-Time PCR Analyzer
available Q1/2015
HUMAN Gesellschaft für Biochemica und Diagnostica mbH Max-Planck-Ring 21 · 65205 Wiesbaden · GermanyTel. +49 6122-9988-0 · Fax +49 6122-9988-100 · e-mail: [email protected] · www.human.de
98
1168
/201
4-07
© 2
014
Hum
an G
mbH
Quantitative Real-Time PCR From sample to result in less than three hours
Nucleic acid isolation
25 min
Set of samples with Real-Time PCR kits
10 min
Curves from a test with HBV-negative samples with additional measurement
Measuring and data collection
120 min