impact II – Bruker Daltonics further improves its omics knowledge generation platforms

At the 62nd ASMS (Baltimore), Bruker is introducing the impact II as the new omics knowledge generation platform.

impact II is the latest evolution of the impact QTOF platform, it provides convincing quantitative proteomics, intact protein analysis and metabolomics capabilities. Its robustness and application oriented bioinformatics software provides even higher efficiency.

This makes the impact II a very versatile and productive platform for omics and biological knowledge generation.

Q-TOF Newsletter

ASMS 2014 Edition

Innovation with IntegrityLC-MS/MS

Combined:

• Accuracy• Speed• Resolution• Robustness

for more Productivity and Versatility in:

• Metabolomics• Intact protein profiling• Quantitative proteomics

Augmented by:

ProteinScapePathwayScreener

ProteinScapePathwayScreener ProteinScapePathwayScreener ProteinScapePathwayScreener

Unique spectral quality

Equipped with the latest of Bruker’s Time-Of-Flight technology, the impact II delivers the best of what only TOF can do: combined resolution, sensitivity,

Dynamic InstantExpertise™ method (compatible with fast LC speed) retains mass accuracy and isotopic fidelity even for low molecular weight and minor intense peaks in MS and MS/MS spectra: the basis for reliable formulae determination of unknowns in metabo-lomics by SmartFormula 3D.

Intensities and CV’s displayed for 10 selected proteins over 55 consecutive injections of Hela Cell line digest. The Low CV (all <10%) over the quantified proteins enables reliable label-free analyses.

RS 19 Ribosomal protein from a 20-min E.Coli cell lysate LC-MS profiling experiment (see corresponding survey view on page 4). Deconvoluted profile MS spectrum is extracted from injection 3. On the left window : BioTools view of a top-down CID MS/MS experiment from an LC-MS/MS experiment run with the same sample and gradient. The average mass error on labelled fragments is 2,45 ppm.

mass accuracy and dynamic range, over the full mass range. Because biology’s diversity demands versatility …

Mass accuracy, Dynamic range and isotopic fidelity @ speed for metabolomics …

... bottom-up proteomics ...

... and top-down analysis

Linking experimental data to biology

Delivering sustainable, quality data of the highest standard is a must have for productivity, but is not sufficient. Bruker’s application bioinformatics transform

the impact II into an omics knowledge generation platform, making the best use of the system’s versatility.

ProteinScape is now Bruker’s central knowledge generation database for proteomics and glycomics. It enables transforming the impact II data into relevant biological knowledge, combining impact II data with information coming from other MS systems and linking it to knowledge databases (3D structures, Gene Ontology, Pride …).

Get twice the answers from Compass PathwayScreener: Analyze the same high resolution full scan LC-MS data set used for a non-targeted evaluation in a pathway driven targeted fashion.

Generate knowledge from protein and glycan analyses ...

... or from pathway driven targeted metabolomics

ProteinScape

•Protein ID, Quant, Characterization, PTM discovery• Includes GlycoQuest

ProfileAnalysis

•The label-free quant tool for non- targeted metabolomics and proteomics

GlycoQuest

•Glycans ID•Glycopeptide screening and characterization

Omics Knowledge Generation

Compass PathwayScreener

•For Pathway-driven targeted metabolomics

Bru

ker

Dal

toni

cs is

con

tinua

lly im

prov

ing

its p

rodu

cts

and

rese

rves

the

rig

ht

to c

hang

e sp

ecifi

catio

ns w

ithou

t no

tice.

© B

DA

L 0

6-2

014,

182

99

06

Bruker Daltonik GmbH

Bremen · GermanyPhone +49 (0)421-2205-0 Fax +49 (0)421-2205-103 sales@bdal.de

Bruker Daltonics Inc.

Billerica, MA · USA Phone +1 (978) 663-3660 Fax +1 (978) 667-5993 ms-sales@bdal.com

www.bruker.com

Fremont, CA · USA Phone +1 (510) 683-4300 Fax +1 (510) 687-1217 cam-sales@bruker.com

Full intact protein profiling of an E.Coli Lysate performed with a 20 min method. RS 19 protein shown on page 2 is extracted from that experiment.

CV’s over 3 technical replicate injections for 78 peptides quantified in plasma. The red line indicates the 10% level. Sample courtesy of Andrew Percy, UBC.

Intact protein profiling Quantitation

The impact II is designed for facilitated use: from the robust and zero adjustment CaptiveSpray nanoBooster source to the self adjusting InstantExpertise™ ID/Quant methods and the simple yet powerful SmartFormula 3D for raw formula generation.

Learn more:

LCMS-81 LCMS-89

Simplicity

Versatility + Productivity = impact II

“We have been using the impact since almost a year for routine shotgun bottom up pro-teomics. In association with the CaptiveSpray nanoBooster, the instrument has provided an excellent level of sustainable performances, being capable of delivering untouched performances for over 6-8 weeks of 24/7 use. This is of tremendous importance for the success of our label-free measurement campaigns.”

Prof. Alain Van Dorsselaer, LSMBO, Strasbourg

Robustness

“The robustness, sensitivity and spectral accuracy of the IMPACT QTOFMS has accelerated the process to identify unknown compounds. This is an integral part of long-term goal to ‘sequence’ the Medicago truncatula metabolome.”

Prof. Lloyd W. Sumner, Analytical Biochemistry Plant Biology Division, The Samuel Roberts Noble Foundation Ardmore, OK, USA

Unknown identification in metabolomics