ProteoRed WG1-WG2 Meeting Pamplona, December 10th 2013 PME10 PROPOSALS.
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Transcript of ProteoRed WG1-WG2 Meeting Pamplona, December 10th 2013 PME10 PROPOSALS.
ProteoRed WG1-WG2 Meeting
Pamplona, December 10th 2013
PME10 PROPOSALS
MSRT1 SIGMA-ALDRICH
TASK M5 M6 M7 M8 M9 M10 M11
STD1: Selection of a peptide standard for normalization of LC retention times (PME10)
STD2: Definition of chromatographic conditions and analysis of the standard.
STD3: Selection and preparation of a target test sample (peptides, cell extract)1.
STD4: SRM analysis of standard spiked sample.
STD5: Data analysis
STD6: Data sharing and final evaluation meeting (ProteoRed PME10)
TASK M5 M6 M7 M8 M9 M10 M11
STD1: Selection of a peptide standard for normalization of LC retention times (PME10)
STD2: Definition of chromatographic conditions and analysis of the standard.
STD3: Selection and preparation of a target test sample (peptides, cell extract)1.
STD4: SRM analysis of standard spiked sample.
STD5: Data analysis
STD6: Data sharing and final evaluation meeting (ProteoRed PME10)
1. Select a cell extract and SRM transitions already described in the consortium.
Objectives:
1) Test Rt standards for SRM methods inter-platform interchangeability
2) Multilaboratory test targeted quantification of Chr16 proteins
Experiment Design:
- Sample: total lysate extract of a model cell line in SpHPP-Chr16 ?
- SRM SpHPP validated methods
- Rt stds- Internal / External - Different gradient lenghts 30-60-90 min
- Labeled peptide standards for selected proteins -> absolute quantitation
PME10