Progress Report 2011 Biofilms

BIOFILMSRESEARCH CENTER FOR BIOINTERFACES

A translation research programme at Malmö UniversityIn partnership with the Knowledge Foundation

Progress report #7JANUARY 1, 2011 - DECEMBER 31, 2011

Biofilms – Research Center for Bioint-erfaces is a translational research programme covering five research groups within three faculties/schools at Malmö University. The core strengths of the Center is i) our broad expertise, spanning the range from theoretical modeling to clinical sciences, and ii) our long experience in working with industry and relating to their needs.

The general aim of the research activi-ties at the Center is to understand, predict and control material/cell/tissue interactions with medical, dental, food and environmental applications. We strive to further integrate education (BSc, MSC, PhD), a cornerstone for the future of the Center. Particularly, one goal is to further integrate Master-level education into our research activities also at an operational level in specific projects.

Biofilms – Research Center for Bioint-erfaces has a goal to become a regional venue for a creative environment of biomedical technology in the Öresund region, facilitating collaboration between academic research, higher education and industry, based on phar-maceutical technology, biotechnology and medical technology, which togeth-er constitutes a key area of significant commercial growth potential. We strive to become a prime entry point for regional industry seeking translational academic expertise in the Biomedical technology field.

CENTER MISSION STATEMENT

Biofilms - Research Center for BiointerfacesProgress Report 2011

Mah: Malmö University; LU: Lund University; KTH: The Royal Institute of Technology; LuTH: Luleå University of Technology; UU: Uppsala University; LiU: Linköping Univer-sity; KI: Karolinska Institute; GU: Gothenburg University; OD: Faculty of Odontology; HS: Faculty of Health and Society; TS: School of Technology. KKs: The Knowledge Foun-dation; VR: The Swedish Research Council; EU: European Union; FP6: 6th Framework Programme; FP7: 7th Framework Programme; SI: Swedish Institute; BMT: Biomedi-cal Technology BMMT: Biomedical Methods and Technology; MS: Materials Science; FLÄK: The Research School in Pharmaceutical Science; BMA: Biomedical Laboratory Science; TELMah: Technology, Economy and Leadership at Malmö University

DIRECTOR´S REPORT 04

LIST OF CENTER MEMBERS 10

LIST OF RESEARCH ACTIVITIES14

RESEARCH HIGHLIGHTS17

SCIENTIFIC INSTRUMENTATION AND EQUIPMENT

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WORKSHOPS AND CONFERENCESORGANISED BY THE CENTER

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PHD THESES SUPERVISED BY CENTER MEMBERS

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COLLABORATIVE PARTNERS OF THE CENTER

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LIST OF PUBLICATION AT THE CENTER FROM 2011 AND ONWARDS

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VISITORS AND SEMINARS AT THE CENTER

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CONTRIBUTIONS TO AND PARTICIPATION IN CONFERENCES AND WORKSHOPS

55

BSc AND MSc EDUCATION60

OTHER ACTIVITIES BY CENTER MEMBERS

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ABBREVIATIONS

CONTENT


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2011 has been a year with many changes for Biofilms – Research Center for Biointerfaces. The research profile grant from the Knowledge Founda-tion came to an end in February and in conjunction with this the manage-ment structure was changed and a new Steering Group and a new Director were appointed. Summing up the year, it can be concluded that the Center has managed the transformation into an independent Center and has succeed-ed in becoming stronger. The Center has extended its industry network, increased the number of researchers, and handled 35 individual research projects. In addition, Center members have been granted more research fund-ing during 2011 than in any year in the past. The Knowledge Foundation granted the Center funding for seven new projects during 2011 and remains an important source of financial support.

The Knowledge Foundation has formally evaluated the research profile investment during 2011 and in the eval-uation it was concluded that research at Biofilms – Research Center for Bioint-erfaces demonstrates a high level of quality. An internationally competi-tive environment has been built up in a field that is highly relevant to industry. This has contributed to the University’s strategic development in research and education, as well as corporate devel-opment.

Lory Melin Svanborg, Jildiz Hamit Eminovski, and Maria Pihl success-

fully defended their PhD theses in 2011. Julia Davies, Håkan Eriksson and Lennart Ljunggren were appoint-ed as Professor and Sergey Shleev was appointed as Associate Professor. Several new members, Per Jönsson, Alexei Iantchenko, and Yuanji Cheng, have been affiliated to the Center. The Center also acquired a new Director on March 28, 2011, when Johan Engblom handed over to Anna Holmberg. Drs Olga Santos, Laura Varas, Jovice Bon Singh, Ida Svendsen and Luis Chavez de Paz left their positions as researchers during the year, while three new post-docs and one PhD student were recruit-ed. Several senior researchers paid visits to universities outside Sweden, including Japan, Russia, Germany and Norway.

The management structure of the Center has been improved during 2011. The Steering Group and the Reference Group have been merged into one board and the Executive Group has been replaced by a “Senior Researchers Forum” (SRF). SRF is a forum for shar-ing knowledge between different disci-plines involved in the Center and one of the main roles of SRF is to identify strategically important research activi-ties and to work together to attract funding for prospective projects. A new Steering Group was appointed by the Vice-Chancellor for the period July 1, 2011–June 31, 2013.

During 2011, we produced 70 publica-tions in international journals as well as ten book chapters and three publica-

THE DIRECTOR’S REPORT


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tions in the popular press. Publications appeared in journals such as Arthritis and Rheumatism (2010 impact factor, 7.3), Biosensors and Bioelectronics (5.4), Acta Biomaterialia (4.8), Bone (4.6), Journal of Physical Chemistry C (4.5), Langmuir (4.3), Electrochemistry Communications (4.3), Electrochimica Acta (3.6), Journal of Physical Chem-istry B (3.6) and Biofouling (3.3). The high impact factors reflect the substan-tial scientific quality of the research carried out at the Center. In addition, 45 oral and 32 poster presentations were made at national and internation-al meetings. A review of the number of citations over the past five years from papers produced by the Center members revealed 8,828 for permanent staff and 1,193 for junior researchers and post-docs. Five members had more than 500 citations each for the period 2007-2011, Ann Wennerberg, Tautgir-das Ruzgas, Gunilla Nordin Fredrik-son, Thomas Arnebrant, and Sergey Schleev. Center members also refereed papers for international journals on a regular basis.

Members of the Center take part in the National Graduate Research School in Odontology (Ann Wennerberg, co-ordinator, Gunnel Svensäter, local co-ordinator and Julia Davies, super-visor). Most researchers participate in undergraduate (BMA and TELMah programmes) and/or graduate teach-ing (BMMT, MS and Dentistry programmes), as well as PhD student supervision. We strive to further inte-grate education (BSc, MSc, PhD), a

cornerstone in the future of the Center. One goal in particular is to integrate Master’s level education even further into our research activities on the oper-ational level in specific projects.

Research collaboration with industry takes place in all four focal areas of the Center. During 2011, we started new research projects with Arla Foods AB, Bioglan AB, Nares AB, Eka Chemi-cals AB, Magle AB, Layer Lab AB, Galenica AB, Novosense AB, Ospol AB, Nobel Biocare AB, and Promimic AB. Although the borders between the focal areas are not always clear cut, the company involvement is as follows: i) Eucaryotic cell-surface interactions (Ospol, Promimic), ii) Molecular trans-port phenomena (AkzoNobel, YKI, ACO Hud Nordic, Eviderm Institute, Nares, Bioglan, Q-Med), iii) Molecular interactions at biointerfaces (Anordica, Acram, Bioglan) and iv) Microbial biofilms (Arla Foods, Acram, Biogaia, Nobel Biocare). We also have close collaboration with Medeon AB and Medicon Valley Alliance.

Center members are partners in the collaborative EU FP7-funded project “Three-dimensional nanobiostruc-ture-based, self-contained devices for biomedical application” (with Sergey Shleev as co-ordinator) and in the EU FP7 Marie Curie Initial Training Network “MagnIM”. We are also involved in three EU Inter-reg programmes; “Medicon FISC”, “Valorisation of knowledge inten-sive ideas in the South Baltic area”


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(SB-VALOR), and “Öresund materials innovation community” (Ö-MIC).

Center members have been responsible for arranging two workshops, “Non-Invasive Biomedical Monitoring and Devices” (Biofilms’ 7th Annual Work-shop), which attracted 100 partici-pants from different universities and industries, and an “Inspiration Day”, an interdisciplinary workshop with 50 participants from industry, as well as partners and Center members. A

symposium “Hydration of nanostruc-tured and biological materials”, which brought together 30 researchers from Malmö University, Vilnius University, St. Petersburg State University and Lund University, was also organised as well as a partner meeting for the project “Humectants and their mechanisms in skin”. Center activities have also been highlighted in other ways, including an article in Dagens Industri and Rapidus Newsletter as well as two press releases and Malmö University newsletters.

Malmö, 29 February 2012Anna Holmberg

Director Biofilms Research Center for Biointerfaces


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The Knowledge Foundation has award-ed research grants for a number of projects that will be run with partners in industry. Biofilms – Research Centre for Biointerfaces will receive SEK 10 million for three research projects.

Anna Holmberg, Director of Biofilms – Research Center for Biointerfaces, is delighted: “This is a great boost. The three research projects that have received funding all fall within our core strengths. The grants mean that we can continue to reinforce and intensify our

research. The projects, which deal with oral implants, tools for non-invasive tests and the interaction of polysaccha-rides with water, involve collaboration with seven different companies, three of which are completely new partners.Anna Holmberg continues: “There has been a steady increase in the number of partners in recent years. At present, we collaborate with 40 different compa-nies and there is growing interest from industry.”

The research project “Devices for

BIOFILMS RECEIVES MAJOR GRANT


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non-invasive biomedical sensing, monitoring and/or delivery” aims in time to offer patients test procedures that cannot be felt. Together with his research colleagues at Health and Soci-ety, Professor Tautgirdas Ruzgas will develop a biosensor that will enable different tests to be carried out with-out troubling the patient. Among other things, the aim is for the biosensors to measure neurotransmitters, such as dopamine, and different hormones. The measurement results from these substances can be used as indicators for cancer, Parkinson’s disease, depres-sion and other conditions. It is difficult to make these measurements at present without troubling the patient. In time, however, researchers hope to be able to offer a more patient-friendly alterna-tive, such as a patch. Professor Ruzgas explains: “In our research project, we will attempt to compensate for individ-ual skin features to allow the patch to be used by everyone, regardless of age or skin type.”

Under the leadership of Senior Lecturer Vitaly Kocherbitov, a research group at Health and Society will study the prop-erties of polysaccharides when they interact with water. Vitaly Kocher-bitov states that polysaccharides, a sugar group, are degradable. “This is a significant advantage when used as a packaging material or a drug carrier. In the research project ‘Carbohydrate polymer - water interactions: sorption, porosity and rheology’ we will collab-orate with industry in these specific areas of use.”

The manner in which the properties of polysaccharides change when they come into contact with water is central – a packaging material, for example, needs to be stable when the humidity changes. In the same way, it must be possible to attach an active substance to a drug carrier outside the body although once it enters the body the substance must be released and the drug carrier needs to degrade. Vitaly Kocherbitov continues: “We can help industry to understand what happens and prepare polysaccharides in such a way that they function in practice. This could be of major significance in a number of areas, the green packaging industry being a good example.”

The research project ‘Oral implants – Nanostructures for the promotion of tissue integration and prevention of infections’ aims to develop better oral implants, thus reducing the risk of infection in the long term. The project is headed by Professor Ann Wenner-berg and Professor Gunnel Svensäter at the Faculty of Odontology. “This is an extremely exciting collaboration project which, apart from working with industry, further enhances the close co-operation that already exists between the research groups.

Oral Biology has the expertise to carry out microbial analyses and at the Department of Prosthetic Dentistry we know about surfaces and tissue inte-gration,” states Professor Wennerberg. Implants need to satisfy two require-ments: tissue integration, which means


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good contact with the cells in the body, and assurance that there is no bacterial adherence to cells, thus avoiding infec-tion. The hypothesis is that the surface of the material will direct bacterial activity. “We’re developing methods for examining bacterial activity and identi-fying any activity the bacteria have in common that could be instrumental in the development of infection.”

Last year, Biofilms secured more than SEK 20 million in research grants. “Things are looking very bright. We have a whole range of activities and by inter-national comparison research within our core strengths is very solid indeed,” concludes Professor Wennerberg.

Malmö University Newsletter, 2012-02-23


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LIST OF CENTER MEMBERS

During the reporting period, the Center comprised the following members (permanent staff, post-docs,

PhD students and technical and administrative staff).

PERMANENT STAFF

Anna HolmbergDr., Director,2011

Johan EngblomAssc. Prof., Director 2008-2011

Thomas ArnebrantProf., Director 2005-2007

Tautgirdas Ruzgas Prof.

Gunilla Nordin FredriksonProf. (also part time LU)

Per Ståhle von SchwerinProf. (also part time LU)

Ann Wennerberg Prof.

Gunnel Svensäter Prof.

Ali Massih Prof.

Julia Davies Prof.

Per Jönsson Prof.

Lennart Ljunggren Prof.

Håkan Eriksson Prof.

Vitaly Kocherbitov Assoc. Prof.

Liselott Lindh Assoc. Prof.

Anette Gjörloff-Wingren Assoc. Prof.

Zoltan Blum Assoc. Prof.

Liu-Ying Wei Assoc. Prof.

Bertil Kinnby Assoc. Prof.

Christina Bjerkén Assoc. Prof.

Claes Wickström Assoc. Prof.

Sergey Shleev Assoc. Prof.

Alexei Iantchenko Assoc. Prof.

Lars Ohlsson Dr.

Tove Sandberg Dr.

Maria StollenwerkDr.

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JUNIOR RESEARCHERS AND POST-DOCS

Anna Ketelsen Dr.

Javier Sotres Dr.

Yuanji Cheng Dr.

Jakob Blomqvist Dr.

Olga Santos, Dr.

Laura Varas, Dr.

Jessica Neilands, Dr.

Luis Chavez de Paz, Dr.

Ida Svendsen, Dr.

Peter Falkman, Dr.

Alejandro Barrantes, Dr.

Jovice Bon Singh Ng, Dr.

Ryo Jimbo, Dr.

Viktor Andoralov, Dr.

Pär Olsson, Dr.

Peter Billsten, Dr.

Justas Barauskas, Dr.

PHD STUDENTS

Jildiz Hamit Eminovski

Ulf Hejman

Alma Masic

Maria Pihl

Sebastian Björklund (employed at LU)

Anton Fagerström

Yana Znamenskaya

Adnan Safdar

Christian Kindblom

Marjan Dorkhan

Kostas Bougas

Magnus Falk

Peter Lamberg

Cathrine Albér

Mariko Hayashi

Lory Melin Svanborg

Tuerdi Maimaitiyili

Zahra El-Schich

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TECHNICAL AND ADMINISTRATIVE STAFF

Eva Nilsson Administrative Co-ordinator

Ulrika Troedsson Technician

Agnethe Henriksson Technician

Madeleine Blomqvist Technician

MSc STUDENTS

Gillian Xi Min Dao

Linda Tran

Jenifer Vallejo

Kouadio Christian Kouassi

Amanda Skurzynska

BMMT Master’s at

HS 2011-2012

Joynul Abedin

Ameena Daftani

Endale Asmare Hailu

Rakibul Islam

Lutfor Islam

Abu Sayeed Khan

Jabed Khandaker

Shadi Movahed Bashiri

Shifa Saleem

Selva Kumar Subramanian

Mohammed B Sunmonu

Inger Anne Tveit

Surendra Vutti

Shaheen Mohammad Syful Islam

BMMT Master’s at

HS 2010-2012

Haddel Ali Shoker

Joy Chia

Ihab Dahi

Payam Delfani

Susanna Tarasco

Mohammad Zahir Uddin

Petra Wicktor

BMMT Master’s at

HS 2009-2011

In addition to these two-year Master’s programmes, the Faculty of Odontology offers the Dentistry programme, which is a continuous, five-year programme to Master’s level.

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MANAGEMENT AND BOARDS

Biofilms – Research Center for Bioint-erfaces is managed on a daily basis by the Center Director and a Senior Researchers Forum, made up of the heads of the individual research groups and senior researchers from the Center. The Steering Group acts as a link to central Malmö University and the community. The Steering Group

comprises the Deputy Vice-Chancellor, the deans of the three faculties/schools involved in the research activities at the Center, representatives from Malmö University’s two research profiles “Biointerfaces” and “Oral Health”, and representatives from industry, Medicon Valley Alliance and other research institutions.

SENIOR RESEARCHERS FORUM

Anna Holmberg:Dr., Director and Chairman, 2011-

Thomas Arnebrant:Prof.

Gunnel Svensäter: Prof.

Ann Wennerberg:Prof.

Tautgirdas Ruzgas:Prof.

Julia Davies: Prof.

Per Jönsson: Prof.

Liselott Lindh: Assoc, Prof.

Bertil Kinnby: Assoc, Prof.

Christina Bjerkén: Assoc, Prof.

Anna Ketelsen: Dr.

Johan Engblom: Assoc, Prof. (substitute)

Claes Wickström: Assoc, Prof. (substitute)

STEERING GROUPEva Engquist:Deputy Vice-Chancellor,Malmö University, Chair

Tapio Salonen:Dean,Faculty of Health and Society

Naser Eftekharian:Head School of Technology,Malmö University

Lars Bondemark:Prof., Dean,Faculty of Odontology

Thomas Arnebrant:Prof., Head of Research Profile,Biointerfaces

Gunnel Svensäter:Prof., Representative Research Profile,Oral Health

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Peter Nordström:Senior Project Manager,Medicon Valley Alliance

Markus Johnsson:Dr., Senior Director,Pharmaceutical Development, Camurus AB

Ulf Brogren:CEO,Promimic AB

Karl Obrant:Head of Research,Skåne University Hospital

LIST OF RESEARCH ACTIVITIES

The research activities of the Center during 2011 can be presented under the following headlines. Projects are listed with partners and funding in brackets. In addition, we are also involved in three EU Inter-reg programmes; “Medicon FISC”, “Valorisation of knowledge intensive ideas in the South Baltic area” (SB-VALOR), and “Öresund materials innovation community (Ö-MIC)”. Center members are partners in the collaborative EU FP7-funded project “Three-dimensional nanobiostructure-based self-contained devic-es for biomedical application” (with Dr Sergey Shleev as co-ordinator) and in the EU FP7 Marie Curie Initial Training Network “MagnIM”.

EUCARYOTIC CELL-SURFACE INTERACTIONS

• Microscopy and digital hologra-phy for real-time applications and imaging: studying the cellular and molecular mechanisms of cancer cells (funded by MAS Cancer foundation)

• Biological responses to photo-reactive hydrophilic nano-size structures (funded by VR)

• Hydrophilic and hydrophobic implant surfaces (funded by implant industry)

• Histological and 3-dimensional analysis of laminin coated polished ceramic implants (funded by Hjal-mar Svensson Research Founda-tion)

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MOLECULAR TRANSPORT PHENOMENA

• The use of CaO as luting material and bone substitute (funded by VR)

• Facilitation of soft tissue healing upon implant treatment in patients with supressed healing ability (funded by Mah)

• Advanced surface characterization of new functional biomaterials (Ospol, funded by KKs)

• The effect of cholesterol-lowering drugs, statins, on cancer cells (funded by Mah)

• Inflammatory mechanism of Al-adjuvants (funded by Mah)

• Adjuvants for products used in agriculture (AkzoNobel Surface Chemistry AB, funded by KKs (until Feb 2011) and Mah)

• Water – a crucial factor in regu-lating biomembrane permeabil-ity (Physical Chemistry 1 (LU), funded by FLÄK, LU)

• Humectants and their mecha-nisms in skin (YKI AB, ACO Hud Nordic AB, Eviderm Institute AB, Q-Med AB, funded by KKs)

• Miniature biofuel cells for self-contained bio-devices: electron transfer in three-dimensional nanobiostructures (funded by VR and EU)

• Grinfeld surface instabilities (funded by VR)

• Effect of a gradient in water chemical potential on buccal drug delivery (Food technology, LU, funded by Mah)

• Mucoadhesion – A prerequisite or a constraint in nasal drug delivery (Nares AB, Bioglan AB, LTH, funded by KKs)

• Development of nanoparticle based enzymatic biofuel cells (Funded by SI)

• Medicon FISC (Foresight Institute + Support Centre), (funded by EU Interreg and Mah).

• Symptomatic vs. asymptomatic atherosclerotic plaques (CRC, LU, funded in part by Mah)

• Biocompatibility of metals (Anor-dica AB & Arcam AB, funded by KKs)

MOLECULAR INTERACTIONS AT BIOINTERFACES

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• Hydration of mucous gel (funded by Mah)

• Topical hydrogen peroxide in wound healing (Bioglan AB, pilot project, funded in part by Mah)

• Membrane impedance spectros-copy as a tool to study skin barrier function and variability (Physi-cal Chemistry 1 (LU), funded by FLÄK (LU) and KKs)

• Development of novel multi-func-tional salivary substitutes for dry mouth syndrome patients (funded by the Swedish Laryng Founda-tion)

• Screening of phase behavior in the DOPS/DOPE/water system and effects on lipid morphology from a decrease in water chemical poten-tial (funded by CLRF)

• Screening of different surface coat-ings including different API for enhanced wound healing (NIOM, Oslo, funded in part by Mah)

MICROBIAL BIOFILMS

• Biologically induced stress corro-sion crack growth (Arcam AB, funded by KKs (until Feb 2011) and Mah)

• Biofilm activity as a marker to identify patients at risk of caries (National Graduate Research School in Odontology, funded by VR and Mah)

• Mucins and microbial biofilms – a symbiotic relationship for health (funded by Magnus Bergvalss Sift-else and Mah)

• Activities of microbial biofilms on bioactive implant surfaces (National Graduate Research School in Odontology, funded by VR and Mah)

• The plasminogen activating system – interaction with microorganisms (Funded by Mah)

• Study of biomarkers and microbial activity at dental implant sites exhibiting healthy bone and severe bone loss (Nobel Biocare, Funded by Mah)

• Biofilms on oral mucosal surfaces (Swedish Dental Association, Swedish Patent Revenue Fund for Research in Preventative Odontol-ogy)

• Milk derived products – a new strategy in oral biofilm control (ArlaFoods, funded by KKs)

• The effect of probiotic lactobacilli on development of acid adapta-tion in dental biofilms (BioGaia, funded by KKs)

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RESEARCH HIGHLIGHTS

Although the application of nanostruc-tures has been recognised as a possible enhancing factor for osseointegration, the detailed mechanisms have not been clarified to prove its initiative role in the osteogenesis cascade. In addition, the in vivo results presented in scientific publications are at times inconsistent, presenting the effect of nanostructures as significant, tendency, or insignifi-cant to osseointegration. One possi-ble explanation for this inconsistency could be that the mode of analysis, which was originally designed to inves-tigate the effect of modification on the micro level, may be too rough for such coarse modification on the nano level. Furthermore, these conventional biomechanical and histological tech-niques may be technically sensitive and the results may vary depending on the operator. Hence, a coarse evaluation that can present absolute values may be necessary to further clarify the effect of nanostructures.

In our study (Jimbo et al. 2011 J Dent Res), we evaluated the genetic respons-es to nanostructured implant surfac-es. In brief, nanostructured calcium phosphate implants were placed in the rabbit tibia. After two and four weeks of insertion, the implants were removal torqued and the implant and the surrounding bone were extracted for gene expression analysis using the real time RT-PCR. The biomechanical results showed that at two weeks the removal torque values were significant-ly higher for the test (nano) compared to the control (turned surface with-out nanostructures), whereas at four weeks, there were no differences between the two groups. The results were in accordance with certain previ-ous studies, where the effect of the nanostructure seemed to diminish at longer time points. However, the investigation using the gene expression approach revealed that even at four weeks, the genetic responses differed

GENETIC RESPONSES TO NANOSTRUCTURED CAP IMPLANTS

Figure1: Gene expression observed by the real-time PCR technique. It is evident that the nanostructured implants enhanced osteogenic marker expression.

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We aim to answer the question “How do changes in the water gradient across skin affect the electrical imped-ance properties of the skin barrier?” We make use of a novel experimental approach, which permits strict control of the boundary conditions that deter-mine the water gradient. A further aim of the study is to exploit measurements using electrical impedance spectrosco-py as a means of explaining diffusion properties of model drugs with different lipophilicity through skin. In conclu-sion, the skin membrane resistance, Rmem, and effective capacitance, Ceff, are affected by varying water gradient

and to some extent the effect is revers-ible. The change in Rmem indicates that the ions inside SC become much more mobile upon hydration, resulting in a distinct decrease in Rmem. The increase in Ceff upon hydration indicates that the dielectric properties of the stratum corneum changes. The effect of water gradient on Rmem and Ceff is related to the flux behaviour of small molecular compounds through skin. When the skin membrane is more hydrated, it has a lower Rmem, a higher Ceff, and higher drug permeability, while the opposite is true when the membrane is less hydrated.

A WATER GRADIENT CAN BE USED TO REGULATE BIOPHYSICAL PROPERTIES OF SKIN: RELATIONSHIP BETWEEN IMPEDANCE AND DRUG PERMEABILITY

Figure1: (A) Schematic representation of the Franz cell equipped with four electrodes for electrical impedance measure-ments. (B) Equivalent electrical circuit used to fit the experi

mental impedance data. Rsol is the resistance of the donor and receptor solution, Rmem is the membrane resistance, and CPE is a constant-phase element.

A. B.

significantly between test and control, and also revealed significantly higher expression of bone-related markers for the test group.

This study showed that the effect of calcium phosphate nanostructures is

significant to osseointegration and that the nanostructures influence the gene expression levels of osteogenic markers. Based on this model, it may be possible to optimise implant nanostructures for further improvement in this field.

Project Partner: Promimic AB

Genetic responses to nanostructured

calcium-phosphate-coated implants. Jimbo

R, Xue Y, Hayashi M, Schwartz-Filho HO,

Andersson M, Mustafa K, Wennerberg A. J

Dent Res 2011;90:1422-1427

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The technique described above involv-ing simultaneous assay of drug pene-tration and monitoring of impedance characteristics of skin was also used to assess permeability properties of biologically active compounds through

leaves and apple cuticle. Hopefully, measurement of impedance character-istics of biological membranes will help to account for the variability in pene-tration assays.

Project Partners: Galenica AB,

Physical Chemistry 1, LU, Dermatology, LU,

Innovator Skåne AB, Akzo Nobel Surface

Chemistry AB, Bayer Crop science A/G

A water gradient can be used to regulate

biophysical properties of skin: relationship

between impedance and drug permeability;

Björklund S, Engblom J, Dahi I, Ruzgas T;

manuscript to be submitted in 2012


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The mouth is an entry point for a huge variety of substances into the human body. As a result, oral surfaces are constantly confronted with different chemical and mechanical challenges due to extrinsic factors that could lead, for example, to dental erosion. Salivary protein films are a major issue in the study of dental erosion as they cover all surfaces within the oral cavity and thus act as a protective barrier. We have used atomic force, microscopy-based friction force spectroscopy (FFS) to study the strength of salivary films. Results revealed that salivary films have strengths in the order of hundreds of MPa. Moreover, our results show that the strength of the adsorbed sali-vary films increases dramatically with the water wettability of the underlying substrates, suggesting that hydropho-bic materials in the oral cavity might be cleaned more easily of adsorbed salivary films. However, FFS also provides information on the structure of these films. From our experiments,

it is inferred that on both hydrophobic and hydrophilic substrata these films can be described in terms of two differ-ent fractions, with only one of them being able to diffuse along the underly-ing substrata. FFS also sheds light on the mechanisms that lead to the loss of the protective function of salivary films when the pH of the surround-ing medium is lowered – a situation that mimics the intake of soft drinks. Specifically, lowering of pH causes desorption of some of the components of the films formed on hydrophobic surfaces, leading to weaker layers. In contrast, at a low pH, saliva no longer forms a layer on hydrophilic silica surfaces. Instead, an inhomogene-ous distribution of amorphous aggre-gates is observed. Finally, reproducible differences are observed in results from experiments on films from different individuals, validating the technique as a tool for clinical diagnosis of the resistance to erosion of salivary films.

FRICTION FORCE SPECTROSCOPY AS A TOOL TO STUDY THE STRENGTH AND STRUCTURE OF SALIVARY FILMS

Figure1: Schematic drawing of an AFM tip scratching a salivary film. The sample corresponds to a real scratched salivary film imaged after FFS measurement.

Friction Force Spectroscopy as a Tool to

Study the Strength and Structure of Salivary

Films. Sotres J., Lindh L.,

Arnebrant T. 2011

Langmuir. 27: 13692-13700

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Some bacteria are able to initiate an acid tolerance response (ATR) on exposure to sub-lethal pH levels which allows them to survive and continue to produce acids at low pH. Recently, there has been a great deal of interest in

whether probiotic bacteria can be used to promote oral health and the aim of this study was investigate the effect of lactobacilli on acid adaptation in the model species, Streptococcus mutans.

Streptococcus mutans UA159 and lactobacilli were allowed to adhere to the surface of a mini-flow cell for two hours (Figure 1) and an ATR was stimulated by exposure to pH 5.5 for two hours. ATR induction was tested by exposing the cells to pH 3.5 for 30 minutes and testing the viability using LIVE/DEAD® BacLightTM staining and CSLM.

Our results showed that Lactobacillus casei UB17 and Lactobacillus reuteri PTA5289, but not Lactobacillus plantarum UA17, were capable of reducing acid adaptation in S. mutans (Figure 2).

THE EFFECT OF LACTOBACILLUS STRAINS ON STREPTOCOCCUS MUTANS ACID ADAPTATION

Figure1: a) LIVE/DEAD BacLight staining of S. mutans and Lactobacillus reuteri, b) phase contrast image showing the differences in morphology used to distinguish between the two species.

Figure2: Graph showing how growth with different Lactobacillus species affects acid tolerance of S. mutans cells.

Jessica Neilands in collaboration with

Biogaia AB.

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Biofilm communities are highly organ-ised structures composed of multiple bacterial species that interact with each other in order to survive. Syner-gistic coexistence of bacteria in multi-species biofilms has been proposed to play a crucial role in the develop-ment of chronic infections as many species become highly resistant to anti-microbial therapy and host defences.

We have developed methodology to study the composition of multi-species communities using 16S rRNA FISH and confocal scanning laser micros-copy (Figure 1a). This technology can be combined with fluorescent probes to investigate the physiological status, for instance activity of protease enzymes, of different cells within a biofilm (Figure 1b).

COMPOSITION AND PROTEASE ACTIVITY IN MICROBIAL BIOFILMS

Figure1(a):Composition of a multi-species biofilm using four different 16S rRNA probes for Streptococcus gordonii (green), Lactobacillus salivarius (red), Enterococcus faecalis (pink) and Actinomyces naeslundii (blue).

Figure1(b):Species-specific 16S r-RNA probes in combina-tion with a fluorescent protease substrate (FITC-casein) showing the physiological status of individual cells of each bacterial species within a 4-species biofilm.

Images: Luis Chávez de Paz

5.


22

Fluorescent substrates can also be used on multi-species biofilms developed in vivo (dental plaque) to investigate the physiological activity of the cells (Figure 2a & b).

Image: Claes Wickström, Bertil Kinnby,

Julia Davies, Gunnel Svensäter,

Wickström C, Herzberg MC, Beighton D,

Svensäter G.

(2009) Microbiology 155:2866-72

Figure2:Plaque biofilms showing proteolytically active (green) and non-proteolytically active cells.

These results show that this is a powerful technique for investigation of the meta-bolic and physiological properties of multi-species biofilms.

Image: Bertil Kinnby

Method development: Claes Wickström,

Bertil Kinnby, Julia Davies and Gunnel

Svensäter in collaboration with Nobel

Biocare AB.


23

Infected root canals contain necrotic tissue as well as tissue fluids derived from inflammatory reaction around the root apex. Under such circumstanc-es, plasma components, such as plas-minogen, may coat the root canal walls and act as a conditioning film to which bacteria can adhere and grow. We have studied the effect of surface-associat-ed plasminogen on the structure and species distribution of a four-species community recovered from infected root canals (Lactobacillus salivarius, Streptococcus. gordonii, Enterococcus faecalis and Actinomyces naeslundii). Bacteria were grown on plasminogen-

or albumin-coated flow chambers and monitored for 96 h with confocal laser scanning microscopy. BacLight LIVE/DEAD staining was used to assess cell viability and 16S rRNA fluorescence in situ hybridisation (FISH) used to study the distribution of the species. After 24 h, no significant difference was seen in the relative amounts of the four species on the two different coatings (Figure 1). Adherence of S. gordonii to the plasmi-nogen-coated surfaces was inhibited by epsilon amino-caprioic acid, indicating that binding is mediated via lysine resi-dues in the molecule.

SURFACE-ASSOCIATED PLASMINOGEN AFFECTS THE POPULATION DIVERSITY IN MIXED-SPECIES BIOFILMS

Figure1:16S rRNA-FISH staining showing the distribution of S. gordonii (green), L. salivarius (red), A. naes-lundii (blue) and E. faecalis (pink) on plasminogen-coated surfaces (upper panel) or albumin-coated surfaces (lower panel) after 24 h and 96 h.

After 96 h, the dominant species on the plasminogen-coated surface was S gordo-nii, whereas on the albumin-coated surface, no single species dominated. Thus, surface-associated plasminogen appears to affect the special distribution of root canal bacteria in ‘mature’ biofilms.

Kinnby B, Chávez de Paz LE,

DaviesJR, Svensäter G.

Protein coating influences biofilm formation

by a root canal consortium.

To be submitted to Microbiology.

6.


24

A major function of the salivary pellicle on oral surfaces is to promote colonisa-tion of the commensal microbiota by providing binding sites for adherence. Streptocoocus mitis is an early colon-iser of the oral cavity while Streptococ-cus mutans represents a later coloniser. As biofilms develop, bacteria undergo adaption to survive in their habitat.

One example of this is the expression of enzymes to allow exploitation of salivary proteins and glycoproteins as a nutrient source. In this study, adher-ence and protease activity of S. mitis biovar 2 and S. mutans were investigat-ed in a flow cell model in the presence of different populations of surface-associated salivary proteins (Figure 1).

HUMAN SALIVARY MUCIN MUC5B-COATED SURFACES ENHANCE PROTEASE ACTIVITY

Figure1:Ibidi flow cell system with a continuous flow of nutrients allows biofilm formation. Confocal microscopy is used to visualise adherence and protease activity.

Figure2:Protease activity in S. mitis biovar 2 is upregulated follow-ing contact with a surface conditioned with human salivary MUC5B mucin. Cells were visualised in a CLSM after stain-ing with FITC Casein and SYTO 62. All cells are stained red and the green halo indicates protease active cells.

In conjunction with adherence to salivary proteins, we demonstrated a rapid up-regulation of proteolytic activity by both streptococcal species. This suggests that both species can sense surface-associated salivary proteins and respond by up-regulating proteases and/or proteolytic activity. This may represent a mecha-nism for the generation of nutrients through the degradation of salivary proteins.

Streptococcus mitis biovar 2 adhered well to surfaces coated with both an MUC5B-enriched fraction or a pool containing MUC7, gp340, amylase, lactoferrin, lysozyme, cystatin, statherin and IgA, whereas adherence of S. mutans was poor. These differences in adhesion may explain in part why S. mitis is an early coloniser of oral surfaces while S. mutans colonises later.

Kindblom C, Davies JR, Herzberg MC,

Svensäter G and Wickström C.

Surface-associated salivary proteins promote

proteolytic activity in Streptococcus mitis

biovar 2 and Streptococcus mutans.

Submitted to Molecular Oral Microbiology

December 2011.

7.


25

Mucins and commensal microorganisms have a strong tendency to associate with surfaces. The formation of adherent microbial communities prevents colonisation by pathogenic bacteria. This study is based on the hypothesis that early colonis-ers, such as Streptococcus gordonii, interact with mucins, resulting in sequential expression of cell wall and other surface proteins that are fundamental to adher-ence and survival of bacteria in the mouth.

CAN BACTERIA SENSE AND RESPOND TO MUCINS ON SURFACES?

Figure1: Cell wall proteins of Streptococcus gordonii.

Four LPXTG-linked proteins (recognised as putative adhesins), glyceraldehyde-3P dehydrogenase (gapdh) and phosphopyruvate hydratase (pph) dominated the cell wall proteome. Proteomic analysis of surface proteins revealed that most were down-regulated, except for superoxide dismutase, which showed significant up-regulation on contact with mucin. These data suggest a role for superoxide dismutase for bacterial survival in initial biofilms.

Method development:

Claes Wickström, Julia Davies, Mark

Herzberg, Agnethe Henriksson, Gunnel

Svensäter.

2DE: Agnethe Henriksson

The primary aim of this study was to establish the cell surface proteome of S. gordonii, including cell wall proteins (Figure 1). Protein extraction proce-dures were developed for cell surface and cell wall proteins and two-dimen-sional electrophoresis was optimised to allow separation of the cell wall proteins. Proteins were identified using mass spectrometry.

Figure2:Surface proteins of S. gordonii in the bulk phase (left) and after adherence to a mucin-coated flow cell. Mucins were prepared from saliva and used to coat flow cell surfaces to which bacteria were allowed to adhere for two hours.

Changes in cell surface protein expression during interaction with mucin-coated surfaces were then investigated (Figure 2).

8.


26

A NOVEL SALIVA-BINDING ADHESIN FROM STREPTOCOCCUS ORALIS

Figure1:2DE of cell wall proteins from three strains of S. oralis. Arrows indicating LPXTG-linked protein. Reference proteins are circled: 1, DnaK; 2, GroEL; 3, trigger factor.

In order to investigate the mechanisms of binding to saliva, bacterial cell wall proteins were isolated and analysed using proteomics (Figure 1). This showed that S. oralis strains bound to saliva expressed an LPXTG-linked protein, which was not present in the

non-adherent strain. Consequently, strains of S. oralis differ in their capaci-ty to bind to saliva-coated titanium and we propose that this is due to differen-tial expression of a novel adhesin. Dorkhan M., Chávez de Paz L. E., Skepö

M., Svensäter G. and Davies J. R. (2012).

Effects of saliva or serum coating on

adherence of Streptococcus oralis strains to

titanium. Microbiol. 159:390-397.

2DE: Agnethe Henriksson

The use of titanium dental implants to treat tooth loss has increased rapidly in recent years. If the implant becomes exposed to the oral cavity, bacteria will adhere to the surface, leading to the formation of microbial biofilms, which can induce peri-implant inflam-mation. Streptococcus oralis is an early coloniser of oral surfaces and has been recovered from titanium surfaces in vivo. The purpose of this study was

to examine the effect of a saliva- or serum-derived coating on adherence of clinical strains of S. oralis to titanium. Three different strains of S. oralis were tested and adherence was studied using a flow cell system and confocal laser scanning microscopy. The results indi-cated that while serum did not promote binding of any studied S. oralis strains, a saliva coating increased adherence in two out of three strains tested.

9.


27

SCIENTIFIC INSTRUMENTATION AND EQUIPMENT

(Contact person: Vitaly Kocherbitov)A Zeiss Axioplan Universal micro-scope equipped with differential inter-ference contrast and a High Resolution Microscopy Camera AxioCam MRm Rev. 3 FireWire, Illuminator HBO 100 as well as a 100W mercury short-arc

lamp and a system of filters to allow fluorescence microscopy observations. The microscope is also equipped with a high-sensitivity SIT video camera and an image processor, AxioVision 4 and Macintosh-based image analysis soft-ware.

(Contact person: Johan Engblom)Three alternative items of thermo-stated in vitro diffusion equipment are available in house, two set-ups with flow-through cells (15 + 7 cells) [R.L. Bronaugh & R.F. Stewart, J. Pharm. Sci. 74 (1985) 64–67] and 10 static Franz cells [Franz T, J. Invest. Dermatol. 64 (1975) 190-195]. Solute

diffusion over skin, oral mucosa, nasal mucosa, nails, plant cuticle and synthetic membranes has been studied and assayed spectrophotometrically (online) or using HPLC-UV. Electri-cal impedance spectroscopy has been appended to the Franz cells as a tool to explain diffusion properties of solutes through these membranes.

(Contact persons: Justas Barauskas, Johan Engblom)Small and Wide Angle X-Ray Scat-tering. Kratky compact camera with line collimation (slit focus), equipped with two linear MBraun detectors

(small and wide angle). Temperature-controlled sample stages (0-70°C or 25-300°C). Powder (semi-solid/solid) or capillary (liquid) sample holders, as well as a flow-through capillary sample holder.

(Contact person: Vitaly Kocherbitov)Differential scanning calorimeter DSC1 from MettlerToledo, equipped with an intracooler TC100 and HSS8 sensor. Temperature range: -90°C to +550°C. The calorimeter can be used

to study solid, soft and liquid substanc-es. Typical sample masses are 2-10 mg. The DSC can be run in a modulation regime to resolve reversing and non-reversing heat effects.

OPTICAL MICROSCOPY

IN VITRO DIFFUSION EQUIPMENT

SWAXD

DSC

1.

2.

3.

4.


28

(Contact person: Alejandro Barrantes)An automated Rudolph thin film ellip-someter (type 43603-200E, Rudolph Research, USA) equipped with a xenon arc lamp as a light source; an interfer-ence filter with UV and infrared block-ing (Melles Griot, The Netherlands) for working at a wavelength of 4429 Å. The experimental set-up is based on null ellipsometry in the PCSA arrange-ment. The components of the ellip-someter are controlled by means of the

Ellipso software, which automatically measures the ellipsometric angles Psi and Delta, allowing the calculation of the thickness, refractive index and adsorbed mass of the growing films. To perform experiments in liquid media, a peristaltic pump (Ole Dich Instruments) is used. The temperature is controlled by means of a Julabo 5B water bath. Data analysis is performed using the Ellipsometry software by Plamen Petrov.

(Contact persons: Zoltan Blum, Justas Barauskas)Scanning Electron Microscope, SEM, Zeiss EVO LS10. Apart from standard modern SEM features, i.e. 5 nm point-to-point resolution at high vacuum and fully conducting samples, the EVO LS10 also offers environmental control (hence “EVO”). By controlled water vapour introduction, with or without the added benefits of a Peltier Cool-

stage, fully hydrated samples can be imaged with accurate sample temper-ature control. Owing to the high output LaB6 filament and the beam sleeve technology, the loss in resolu-tion is only tenfold. The instrument is equipped with the appropriate detec-tors as well as an INCA EDX microa-nalysis system.

(Contact person: Alejandro Barrantes)The Q-Sense E4 quartz crystal micro-balance is equipped with the E4 meas-uring chamber that allows up to four experiments in the four temperature-controlled cells to be run simultaneous-ly. Changes in frequency and dissipation are registered by means of the E-series electronic unit, which is also respon-sible for temperature control. These changes are related to the adsorbed wet

mass and the viscoelastic properties of the films adsorbed onto the sensor. The QCM-D is also equipped with an electrochemistry and an ellipsometry module, enabling the simultaneous measurement of the electrochemical and optical properties of the adsorbed films. There is a four-channel IPC peri-staltic pump (Ismatec). Data analysis is performed using the Q-Tools software (Q-Sense).

ELLIPSOMETER

e-SEM

QCM-D

5.

6.

7.


29

(Contact person: Peter Lamberg)A combined Electrophoretic Light Scattering/Dynamic Light Scattering Zeta potential/particle sizing system with a 5 MW 632.8 nm HeNe laser, two insertable electrodes for high or low voltage, covering possible electrical

field strengths between 0.25-250 V/cm, and an adjustable temperature range of 4-100°C. The instrument has also been fitted with a Thermaltake Bigwater 760is liquid cooling system, to prevent cuvette fogging at high temperatures.

(Contact person: Vitaly Kocherbitov)Kruss Goniometer DSA100 Drop Shape Analyser System for measurement of static and dynamic contact angles. The goniometer is equipped with a single

dosing system DS3200/3201. Tempera-ture is controlled in the range 5-90°C using a TC30 measuring chamber. Humidity is controlled using a HC10 humidity chamber.

(Contact persons: Javier Sotres, Thomas Arnebrant)A Multimode SPM equipped with a Nanoscope VIII control unit (Bruker AXS). The SPM is also equipped with a PicoForce Unit, a Heater Unit, and

the ScanAsyst and Peak Force Tapping software (Bruker AXS). In addition, a digital oscilloscope (Tektronix TDS 2022C) and a Nikon optical micro-scope are connected to the system.

(Contact person: Tautgirdas Ruzgas)Franz cells (Ø = 0.90 cm, V = 6 ml, PermeGear, Hellertown, PA, USA) equipped with four electrodes and connected to a potentiostat from Ivium Technologies (Eindhoven, The Neth-erlands) are used to study impedance characteristics of membranes, includ-

ing skin, leafs, etc. Two platinum wires serve as working and counter electrodes and two Ag/AgCl/3M KCl electrodes from World Precision Instru-ments (Sarasota, FL, USA) are used as sensing and reference electrodes. The equipment allows impedance measure-ments at frequencies up to 6 MHz.

DLS/ZETA SIZING SYSTEM

GONIOMETER

AFM

IMPEDANCE MEASURING EQUIPMENT

8.

9.

10.

11.


30

(Contact person: Tautgirdas Ruzgas)Drop Volume Tensiometer TVT 2 consists of a step motor-driven syringe, including a needle for droplet forma-tion, set up for optical droplet falling

registration, and software to operate hardware and calculate the surface tension of liquid. This apparatus is used to determine the surface tension of liquids.

(Contact person: Johan Engblom)KSV 5000 is a programmable Lang-muir and Langmuir-Blodgett instru-ment for automated Langmuir film experiments and for deposition of

normal or alternating multilayers onto solid substrates. Surface pressure is measured using the Wilhelmy plate method.

(Contact person: Tautgirdas Ruzgas)Particle micro-electrophoresis appa-ratus Mark II (Rank Brothers, Cambridge, UK) consists of a flat, electrophoretic cell equipped with two platinum electrodes and optical micro-scope. The apparatus can be used to

determine particle electrophoretic mobility in solution. The mobility can be used to assess particle zeta potential and surface charge density. The appa-ratus allows measurements with parti-cles larger than 0.3 μm in diameter.

(Contact persons: Vitaly Kocherbitov, Alejandro Barrantes)Rheometer Bohlin CVO 100 Digital equipped with Peltier Cylinder C25. The rheometer system is suitable for

research, product development and quality control. The rheometer is equipped with 20 mm parallel plate, cone 4/40 and coaxial cylinder meas-uring systems.

(Contact person: Vitaly Kocherbitov)A double-twin sorption calorimeter for the study of hydration of powders and soft materials. The channel diameter is 28 mm. The calorimeter is equipped with two sorption cells, each consist-ing of a sorption chamber (on top)

and a vaporisation chamber (bottom). The chambers are connected by tubes with varying diameters to control the vapour flow. The double twin calorim-eter is inserted into an isothermal TAM 2277 calorimeter from Thermometric, equipped with a nanoamplifier.

DROP VOLUME APPARATUS

LANGMUIR SURFACE BALANCE

PARTICLE ELECTROPHORESIS

RHEOMETER

SORPTION CALORIMETER

12.

13.

14.

15.

16.


31

(Contact persons: Ryo Jimbo, Ann Wennerberg)White light microscopy, horizontal resolution 0.3 μm, vertical 0.1 nm,

equipped with a CCD camera, operat-ing also in phaseshift mode. The main application is topographical characteri-sation on the μm and sub-μm level.

(Contact person: Gunnel Svensäter)FPLC

Preparative electrophoresUltracentrifuge

(Contact person: Anette Gjörloff Wingren)High-performance Western blot and gel imaging. A self-contained digital darkroom, compact design with state-of-the-art CCD optical technology, an integrated computer and simplified

touch screen control. The FluorChem E is built with imaging speeds and a dynamic range that surpass film for low femtogram detection of proteins and DNA in chemiluminescent, colori-metric and UV fluorescent gels and blots.

(Contact person: Gunnel Svensäter)AutoclavesAnaerobic chamberFluorostar

IncubatorsSpectrophotometerPCRCentrifuges

(Contact person: Gunnel Svensäter)Epifluorescence microscopeConfocal laser scanning microscope

Flow cells with controlled continuous flowCell cultures

INTERFEROMETER

PURIFICATION OF MUCINS AND PROTEINS

FLUORCHEM E

MICROBIAL DIAGNOSTICS; SPECIES AND STRAIN LEVELS

BIOFILM FORMATION, INCLUDING ADHERENCE, DETACHMENT, POPULATION DIVERSITY AND PHENOTYPIC SWIFT

17.

18.

19.

20.

21.


32

(Contact person: Gunnel Svensäter)Immunohistochemistry

Western blotsELISA

(Contact person: Gunnel Svensäter)Multiphor

Two-dimensional electrophoresisSonicator

IDENTIFICATION OF PROTEINS

PROTEOMICS, INCLUDING RADIOACTIVE LABELLING OF PROTEINS

22.

23.


33

During 2011 the Center launched the 7th Annual Workshop. The focus this year was “Non-Invasive Biomedi-cal Monitoring and Devices” and the event attracted 100 participants from different universities as well as indus-try. The workshop was preceded by a PhD student day with Professor Pentti Tengvall, Gothenburg University, and Professor Marie Wahlgren, Lund University, as mentors.

The Center also launched an “Inspira-tion Day”, an interdisciplinary work-shop for sharing research progress, at Malmö University. The event attracted 50 participants from industry, both partners and Center members.

Vitaly Kocherbitov, who is co-ordi-nating the project “Hydration of nanostructured and biological materi-als”, brought researchers together for

a symposium at Malmö University. Around 30 researchers from Malmö University, Vilnius University, St. Petersburg State University and Lund University came together to exchange experience within the field of hydra-tion research. Johan Engblom organ-ised a partner meeting for the project “Humectants and their mechanisms in skin” at YKI AB. Johan Engblom also arranged a workshop with AkzoNo-bel and Bayer Crop Science at Malmö University. Peter Falkman arranged a training session on Intellectual prop-erty rights at Malmö University.

Our internal seminars were organised by Tautgirdas Ruzgas, Anette Gjör-loff Wingren, Julia Davies, and Ann Wennerberg. Gunilla Nordin Fredrik-son was responsible for the seminars at the Clinical Research Center, Lund University.

WORKSHOPS AND CONFERENCES ORGANISED BY THE CENTER

Each year, Biofilms - Research Center for Biointerfaces holds a workshop that addresses researchers, industry and other principal figures in the biomedi-cal field. This year, the topic was “Non-invasive Biomedical Monitoring and Devices”.

The annual Biofilms workshop this year attracted almost 100 guests from different universities and companies. During the two-day workshop, a wide range of interesting lectures were held

on the topic of non-invasive biomedical monitoring and devices with a special focus on skin, saliva and tears. This is an area in which research is moving rapidly forward.

“We are very happy to present our research in the field and at the same time have the opportunity to listen to many interesting lecturers from indus-try,” says Anna Holmberg, Director of Biofilms - Research Center for Bioint-erfaces.

SKIN, SALIVA AND TEARS


34

During the workshop, prominent researchers as well as representatives from industry talked about new meth-ods for measuring, monitoring and making diagnoses from skin, saliva and tears. Erik Thomsen, researcher at DTU Nanotech, lectured on the devel-opment of an electronic patch that can measure heart beat rate, oxygen satura-tion, and blood pressure. John McCor-mac, Mini FAB, explained about a device that can be used to sample small volumes of tears and that can be used to make a diagnosis.

From the Faculty of Health and Society, researcher Sergey Shleev talked about the development of an electrochemical bio-device that can be used to meas-

ure different substances in tears. Taut-girdas Ruzgas, also from the Faculty, lectured on how to study skin perme-ability using impedance spectroscopy.

“This is the third time I have participat-ed and I find the workshop very interest-ing. The wide range of lectures provides a good insight into what is new,” says Jan Alenfall, CEO of DemaGen.

Anna Karin Morén, product developer at Galenica, is also very positive.

“The interdisciplinary arrangement and the wide range of applications that have been presented are very interest-ing,” she says.

Malmö University Newsletter,

2011-10-31


35

PHD THESES SUPERVISED BY CENTER MEMBERS

Lory Melin SvanborgMah (Supervisors Ann Wennerberg, Martin Andersson):“On the importance of nanometer structures for implant incorporation in bone tissue”(Dissertation 2011-06-01)

Kostas BougasMah (Supervisors Ann Wennerberg, Pentti Tengvall (GU), Victoria Franke Stenport (GU)):“Protein coat and bone incorporation”(2009-)

Maria WigrenLU (Supervisor Gunilla Nordin Fredrikson):“Protective Immunity in Atherosclerosis”(Dissertation 2011-04-15)

Katarina BergLU (Supervisor Gunilla Nordin Fredrikson):“Lyso-PC and macrophage interplay”(Dissertation 2011-05-20)

Christel LarssonMah (Supervisor Per Vult von Steyern, Ann Wennerberg):“Zirconium dioxide based dental restorations”(Dissertation 2011-05-20)

Jildiz Hamit EminovskiMah (Supervisors Thomas Arnebrant and Krister Eskilsson (Kemira AB)):“Interactions of biopolymers and metal complexes at biological interfaces”(Dissertation 2011-09-15)

Maria PihlMah (Supervisors Gunnel Svensäter, Bertil Kinnby, Thomas Arnebrant):“Microbial Biofilms on Peritoneal Dialysis Catheters”(Dissertation 2011-03-25)

Daniel KolbusLU (Supervisor Gunilla Nordin Fredrikson):“Adaptive immune responses in atheroscle-rosis”(Dissertation 2011-03-17)

Liselott Ellmarker-LöfquistMah (supervisors: Liselott Lindh, Björn Söderfeldt):“A Clinical and subjective evaluation of single implant treatments. A retroscpective study.”(2009-)

THESES DEFENDED:

THESES IN PROGRESS:


36

Ulf HejmanMah (Supervisors Per Ståhle, Christina Bjerkén):“Biologically induced stress corrosion”(2005-, Lic thesis defended 2010, doctoral thesis will be defended autumn 2012)

Adnan SafdarLU (Supervisors Liu-Ying Wei, Per Ståhle):“Biocompatibility of ion beam melted materials”(2008-, Lic thesis defended Dec 2010, doctoral thesis will be defended spring 2012)

Alma MasicMah (Supervisors Per Ståhle, Johan Helsing):“Mathematical modeling of biofilms”(2007-, doctoral thesis will be defended spring 2012)

Sebastian BjörklundLU (Supervisors Emma Sparr (LU), Johan Engblom (Mah) and Krister Thuresson (Camurus AB)):“Water - a crucial factor in regulating biomembrane permeability”(2008-)

Anton FagerströmMah (Supervisors Johan Engblom, Vitaly Kocherbitov and Karin Bergström (AkzoNobel)):“Bioavailability of active ingredients used in agriculture”(2009-)

Yana ZnamenskayaMah (Supervisors Vitaly Kocherbitov and Johan Engblom):“Hydration of mucous gel”(2009-)

Christian KindblomMah (Supervisors Gunnel Svensäter, Claes Wickström, Madeleine Rohlin):“Biofilm activity as a marker to identify patients at risk – mechanisms underlying microbial stress tolerance”(2009-)

Marjan DorkhanMah (Supervisors Julia Davies, Gunnel Svensäter, Ann Wennerberg):“Activities of microbial biofilms on bioac-tive implant surfaces”(2009-)

Magnus FalkMah (Supervisors Sergey Shleev, Tautgirdas Ruzgas,):“Three-dimensional nanobiostructure-based self-contained devices for biomedical application”(2009-)

Jon LindLTH (Supervisors Ali Massih, Christina Bjerkén):“Methods for evaluation of evaluation of the hydride embrittlement of Ni-based super alloys” (2009-)


37

Tuerdi MaymaytilliLTH (Supervisors Christina Bjerkén, Per Ståhle):“Influence of plastic deformation on the formation and growth of embritteling metal hydride’s”(2009-)

Mariko Hayashi(Supervisors: Liselott Lindh, Ann Wennerberg, Ryo Jimbo):“Biological Responses to Amphiphilic Nano-size Structures”(2010-)

Peter LambergMah (Supervisors: Tautgirdas Ruzgas, Thomas Arnebrant):“Three-dimensional (3D) bioelectrochemi-cal systems assembled from nanoparticles”(2010-)

Cathrine AlbérMah (Supervisors: Johan Engblom, Vitaly Kocherbitov, Marie Lodén, Robert Corkery):“Humectants and their effects on skin barrier function – a mechanistic approach”(2010-)

Caitriona GrönbergLU (Supervisor: Gunilla Nordin Fredrikson):“The role of IL-16 in atherosclerosis devel-opment”(2010-)

Polyxeni MantaniLU (Supervisor: Gunilla Nordin Fredrikson):“Atheroprotective humoral and T cell immune responses against oxidized LDL”(2010-)

Sara RattikLU (Supervisor: Gunilla Nordin Fredrikson):“The role of IL-22 in atherosclerosis devel-opment”(2011-)

Zahra El-SchichMah (Supervisors: Anette Gjörloff Wingren, Anna Ketelsen, Gunilla Nordin Fredrikson):“Microscopy and digital holography for real-time applications and imaging: study-ing the cellular and molecular mechanisms of inflammatory cells and cancer cells”(2011-)

THESES IN PROGRESS:


38

COLLABORATIVE PARTNERS OF THE CENTER

ACO Hud Nordic AB

Akzo Nobel AB

Anordica AB

AnoxKaldnes AB

Acram AB

Arla Foods AB

Biogaia AB

Bioglan AB

Bioinvent International AB

Camurus AB

Euro-Diagnostica AB

Eka Chemicals AB

Eviderm Institute AB

Galenica AB

Gambro Lundia AB

InnoScandinavia AB

Innovator Skåne AB

Kemira AB

Layer Lab AB

Magle AB

Medeon AB

Medicon Valley Alliance

MiniFAB, Australia

Nares AB

NIL Technology A/S, Denmark

Nobel Biocare AB

Novosense AB

Novozymes A/S, Denmark

Obducat Technologies AB

Phase Holographic Imaging AB

Promimic AB

PVA-MV AG, Germany

Q-Med AB

QuNano AB

Quantumwise A/S, Denmark

Snemca Moteur, France

Stora Enso AB

Studsvik Nuclear AB

TetraPak AB

Volvo Aero AB

YKI AB

Ospol AB

INDUSTRY PARTNERS 1.


39

ACADEMIC COLLABORATORS

COLLABORATIONS WITH OTHER UNIVERSITIES AND RESEARCH INSTITUTIONS

IN SWEDEN

• Prof. em. Kåre Larsson, Camurus Lipid Research FoundationLund University

• Prof. Gunnar Bergenholtz, Dept. of Endodontics University of Gothenburg

• Prof. Gunnar Dahlén, Dept of Oral Microbiology, University of Gothenburg

• Prof. Ingegerd Johansson, Dept of Cariology, Umeå University

• Prof. Artur Schmidtchen, Department of Clinical Sciences, Lund University

• Prof. Swante Twetman, Specialist Clinic for Oral Health Care, Hallands Läns Landsting, Halmstad

• Prof. Kristian Reisbeck, Dept of Medical Microbiology, Lund University

• Prof. Per Claesson, The Royal Institute of Technology and Surface Chemistry Institute, Stockholm

• Prof. Lo Gorton, Biochemistry, Lund University

• Prof. Christer Hansson, Dermatology, Lund University

• Prof. Martin Malmsten, Pharmacy, Uppsala University

• Prof. Jan Nilsson, CRC UMAS, Lund University

• Prof. Tommy Nylander, Physical Chemistry 1, Lund University

• Prof. Per Uvdal, MAX lab, Lund University

• Prof. Martin Andersson, Dept Applied Chemistry, Chalmers University of Technology

• Prof. Pentti Tengvall, Dept Biomaterials, Sahlgrenska Academy, Gothenburg University

• Prof. Tomas Albrektsson, Dept Biomaterials, Sahlgrenska Academy, Gothenburg University

• Prof. Marie Wahlgren, Food Technology, LTH, Lund University

• Prof. Lars Montelius, Solid State Physics, Lund University

• Prof. Ulf Ryde, Department of Theoretical Chemistry, Lund University

• Prof. Olle Melander, CRC, Lund University Assoc. Prof. Lars Norlén, Cell- and Molecular biology, Karolinska Institute

• Assoc. Prof. Ola Bergendorff, Dermatology, Lund University

2.


40

• Assoc. Prof. Emma Sparr, Physical Chemistry 1, Lund University

• Assoc. Prof. Peter Siesjö, Department of Clinical Sciences, BMC, Lund University

• Dr. Karina Persson, Inst. of Odontology, Umeå University

• Dr. Christer Wingren, LTH, Lund University

• Dr. Lars G Petersson, Specialist Clinic for Oral Health Care, Hallands Läns Landsting, Halmstad

• Dr. Yngve Cerenius, MAX lab, Lund University

• Dr. Adam Feiler, The Royal Institute of Technology and Surface Chemistry Institute, Stockholm

• Dr. Isabel Goncalves, Ola Bergendorff, CRC UMAS, Lund University

• Dr. Robert Corkery, The Royal Institute of Technology and Surface Chemistry Institute, Stockholm

• Dr. Anna Westerlund, Odontology, Gothenburg University

• Dr. Ivan Maximov, Solid State Physics, Lund University

• Dr. Jenny Liao Persson, Center for Molecular Pathology, Lund University

• Dr. Birgit Brandner, Surface Chemistry Institute, Stockholm

• Dr. Tomás Plivelic, MAX lab, Lund University

• Björn Svensson, Specialist docent, Clinic for Oral Health Care, Örebro Läns Landsting, Örebro

• Per Rabe, Specialist Clinic for Oral Health Care, Hallands Läns Landsting, Halmstad

• Brånemark Clinic, Gothenburg


41

INTERNATIONAL COLLABORATIONS

• Prof. Arunas Ramanavicius, Center of Nanotechnology and Material Science, Faculty of Chemistry, Vilnius University, Vilnius, Lithuania

• Prof. Juozas Kulys, Department of Enzyme Chemistry, Institute of Biochemistry, Vilnius, Lithuania

• Prof. Renata Bilewicz, Faculty of Chemistry, University of Warsaw, Warsaw, Poland

• Prof. em. Ian Hamilton, University of Manitoba, Dept of Oral Biology, Winnipeg, Canada

• Prof. David Beighton, Guy’s Kings and St Thomas’ Dental Institute, Joint Microbiology Research Unit, London, UK

• Prof. Mark Herzberg, Department of Microbiology, University of Minnesota, Minneapolis, USA

• Prof. Jukka Meurman, Dept of Oral and Maxillofacial Diseases, Helsinki University Central Hospital, Helsinki, Finland

• Prof. Dennis Cvitkovitch, Dept of Microbiology, University of Toronto, Canada

• Prof. Jeannine Brady, Dept of Oral Biology, University of Florida, Gainesville, USA

• Prof. Phil Marsh, Health Protection Agency, Porton Down, UK

• Prof. Deirdre Devine, Leeds Dental Institute, UK

• Prof. Leslie Banks-Sills, Cornell University, Ithaca, NY, USA and University of Tel Aviv, Israel

• Prof. Jon E Dahl, NIOM, Oslo, Norway

• Prof. Iwona Beech, University of Portsmouth, UK

• Prof. Regine Willumeit, GKSS Research Centre, Geesthacht, Germany

• Prof. Christopher Exley, Keele University, UK

• Prof. Takashe Sawase, Dept Prosthodontics, University of Nagasaki, Japan

• Prof. Wolfgang Schuhmann, Ruhr-Universität Bochum, Germany

• Prof. Edmond Magner, University of Limerick, Ireland

• Prof. Dietmar Haltrich, Universität für Bodenkultur Wien, Austria

• Prof. Phil Bartlett, The University of Southampton, UK

• Prof. Kamal Mustafa, University of Bergen, Norway

• Prof. Alexander Yaropolov, Inst. of Biochemistry, Moscow, Russia


42

• Assoc. Prof. Marie Ranson, School of Biological Sciences, Scientific Director – Cancer, Illawarra Health and Medical Research Institute, University of Wollongong, Wollongong, Australia

• Assoc. Prof. Duncan Sutherland, iNano Centre, Aarhus University, Denmark

• Assoc. Prof. Tim Tolker-Nielsen, University of Copenhagen, Denmark

• Assoc. Prof. David Thornton, University of Manchester, UK

• Assoc. Prof. Pablo Galindo, Department of Oral surgery and Implant Dentistry, Granada University, Spain

• Dr. Vladimir A. Serezhenkov, N.N. Semenov Institute of Chemical Physics, Russian Academy of Sciences, Moscow, Russia

• Dr. Xiaoju Wang, Laboratory of Inorganic Chemistry, Process Chemistry Centre, Åbo Akademi University, Turku, Finland

• Dr. Hirotoshi Matsumurad, Department of Biomaterials Sciences, Graduate School of Agriculture and Life Sciences, The University of Tokyo, Tokyo, Japan

• Dr. Roland Ludwig, Research Centre Applied Biocatalysis, Graz, Austria

• Dr. Lubomír Rulíšek, Institute of Organic Chemistry and Biochemistry, Gilead Sciences Research Center at IOCB, Academy of Sciences of the Czech Republic, Praha, Czech Republic

• Dr. Peter Wejse, ArlaFoods, Aarhus, Denmark

• Dr. Sergei Lobov, Browitt Nanoparticle Lab, Dept of Applied Mathematics, Australian National University, Canberra, Australia

• Dr. Miguel Alcalde, Consejo Superior de Investigaciones Científicas, Applied Biocatalysis group, Spain

• Dr. Antonio L. De Lacey, Consejo Superior de Investigaciones Científicas, Bioelectrocatalysis group, Spain

• Dr. Donald Leech, National University of Ireland, Galway, Ireland

• Dr. Paulo G. Coelho, Department of Biomimetics, New York University, USA

• Dr. Yuusuke Kozai, Department of Radiology, Kanagawa Dental University, Japan

• Dr. Humberto Schwartz, Sao Paolo, Brasilien

• Dr. Jan-Tore Samuelsen, NIOM, Oslo, Norway


43

LIST OF PUBLICATIONS AT CENTER FROM 2011 AND ONWARDS

All titles listed under journal articles, review papers, and book chapters have been or are currently subject

to peer review. Center publications for 2010 can be found at http://www.mah.se/biofilms

• Bergenzaun, L., Gudmundsson, P., Öhlin, H., Düring, J., Ersson, A., Ihrman, L., et al. (2011). Assessing left ventricular systolic function in shock: Evaluation of echocardiographic param-eters in intensive care. [elektronisk resurs]. Critical care;4 Biomed Centralw Ltd.

• Beyl Y, Guschin D A, Shleev S, Schuhmann W, A chloride resistant high potential oxygen reducing biocathode based on a fungal laccase incorporated into an optimized Os-complex modified redox hydrogel. Electrochemistry Communications, 2011, 13(5), 474-476.

• Björkbacka H, Fredrikson GN and Nilsson J. Beyond CRP - looking for biomarkers for immune modulators of inflammation, J Am Coll Cardiol, pending revision.

• Björklund, Sebastian and Wadsö, Lars A calorimetric method to determine water activity, REVIEW OF SCIENTIFIC INSTRUMENTS 82, 114903 (2011)

• Bonfante EA, Granato, R, Marin C, Jimbo R, Giro G, Suzuki M, Coelho, PG. Biomechanical testing of microblasted, acid-etched/microblasted, anodized, and discrete crystalline deposi-tion surfaces. An experimental study in beagle dogs. Int J Oral Maxillofac Impl.MENTS 82, 114903 (2011)

• Browaeys H, Vandeweghe S, Johansson CB, Jimbo R, Deschepper E, De Bruyn H. The histo-logic evaluation of osseointegration of Osseotite and Nanotite microimplants immediately loaded in conjunction with sinus lifting in humans. Clin Oral Implants Res, In Press.

• Chávez de Paz LE, Lemos JA, Wickström Cand Sedgley CM. Role of (p)ppGpp in biofilm formation by E. faecalis Appl Environ Microbiol 2011, Accepted for publication.

• Chowdhary R, Jimbo R, Scharfe Thomsen C, Carlsson L, Wennerberg A. Biomechanical evaluation of macro and micro designed screw-type implants: An insertion torque and removal torque study in rabbits. Clin Oral Implants Res, Accepted for publication.

• Coelho PG, Granato R, Marin C, Jimbo R, Lin S, Witek L, Suzuki M, Bonfante EA. Effect of Si Addition on Ca- and P- Impregnated Implant Surfaces with Nanometer Scale Roughness. An Experimental Study in Dogs. Clin Oral Impl Res; 2011, Available on early viewpublication.

• Del Corso M, Vervelle A, Simonpieri A, Jimbo R, Inchingolo F, Sammartino G, Dohan Ehren-fest DM. Current knowledge and perspectives for the use of Platelet-Rich Plasma (PRP) and

JOURNAL ARTICLES AND INVITED REVIEW PAPERS IN JOURNALS

1.


44

Platelet-Rich Fibrin (PRF) in oral and maxillofacial surgery. Part 1: periodontal and dentoal veolar surgery. Current Pharmaceutical Biotechnology, Accepted for Publication.

• Dohan Ehrenfest DM, Bielecki T, Jimbo R, Barbé G, Marco Del Corso M, Inchingolo F, Sammartino G. Do the fibrin architecture and leukocyte content influence the growth factor release of platelet concentrates? An evidence-based answer comparing a Pure Platelet-Rich Plasma (P-PRP) Gel and a Leukocyte- and Platelet-Rich Fibrin (L-PRF). Current Pharmaceuti-cal Biotechnology, Accepted for Publication

• Dorkhan M, Chávez de Paz LE, Skepö M, Svensater G and Davies JR. (2012). Effects of saliva or serum coating on adherence of Streptococcus oralis strains to titanium. Microbiol 158; 390-397.

• Dunér P, To F, Björkbacka H, Fredrikson GN, Nilsson J, Bengtsson E. Immunization of apoE-/- mice with aldehyde-modified fibronectin inhibits the development of atherosclerosis. Cardio-vasc Res, 91(3):528-36, 2011.

• Engelbertsen D, Anand DV, Fredrikson GN, Hopkins D, Corder R, Shah PK, Lahiri A, Nilsson J, Bengtsson E. High levels of IgM against methylglyoxal-modified apolipoprotein B 100 is associated with less coronary artery calcification in patients with type 2 diabetes. J Int Med, in press. doi: 10.1111/j.1365-2796.2011.02411.x. [Epub ahead of print]

• Falk M., Blum Z., Shleev S. Direct electron transfer based enzymatic fuel cells. Invited review. Electrochimica Acta, 2012, Accepted for Publication

• Falkman P, Åberg C, Clemens A, and Sparr E, Lyotropic lipid phases confined in cylindrical pores: structure and permeability, J Phys Chem B 115(49), 14450-61, 2011

• Fransson H, Petersson K, Davies JR. Dentine sialoprotein and collagen I expression after experimental pulp capping in humans using emdogain gel. Int Endod J 2011, 44:259-267.

• Fröjd V, Chávez de Paz LC, Andersson M, Wennerberg A, Davies JR and Svensäter G. In situ analysis of multispecies biofilm formation on customized titanium surfaces. Mol Oral Micro-biol, 2011, 26:241-252

• Fröjd V, Linderbäck P, Wennerberg A, Chávez de Paz L, Svensäter G and Davies JR. Effect of nanoporous TiO2 coating and anodized Ca2+ modification of titanium surfaces on early micro-bial biofilm formation. BMC Oral Health. 2011 Mar 8;11:8.

• Gaidamauskas E., Rynkun P., Nazé C., Gaigalas G., Jönsson P., and Godefroid M., Tensorial form and matrix elements of the relativistic nuclear recoil operator, Journal of Physics B, 44 175003 (2011).

• Gonçalves I, Stollenwerk M, Lindholm MW, Dias N, Pedro L, Fernandes e Fernandes J, Moses J, Fredrikson GN, Nilsson J, Ares MPS. Activator Protein-1 in Carotid Plaques is related to Cerebrovascular Symptoms and Cholesteryl Ester Content. Cardiovasc Pathol, Jan-Feb;20(1):36-43, 2011.

• Halgren C, Kjaergaard S, Bak M, Hansen C, El-Schich Z, Anderson C, Henriksen K, Hjalgrim H, Kirchoff M, Bijlsma E, Nielsen M, den Hollander N, Ruivenkamp C, Isidor B, Le Caignec C, Zannolli R, Mucciolo M, Renieri A, Mari F, Anderlid BM, Andrieux J, Dieux A, Tommerup N, Bache I., Corpus callosum abnormalities, intellectual disability, speech impairment, and autism in patients with haploinsufficiency of ARID1B., Clin Genet. 2011 Jul 29. doi: 10.1111/j.1399-0004.2011.01755.x. [Epub ahead of print]


45

• Halldin A, Jimbo R, Johansson CB, Wennerberg A, Jacobsson M, Albrektsson T, Hansson S. The effect of increased installation torque on implant stability. Bone 2011 Oct; 49(4): 783-9.

• Hejman U, Bjerken C, Dissolution driven crack branching in polycarbonate. FATIGUE & FRACTURE OF ENGINEERING MATERIALS & STRUCTURES Volume: 34 Issue: 4 Pages: 227-239

• Henssge U, Do T, Gilbert SC, Clarke D, Wickström C, Ligtenberg AJM, Radford D and Beighton D. Application of multi-locus sequence typing and pilus gene (fimA and fimP) sequence compari-sons to investigate the population structure in Actinomyces naeslundii and Actinomyces oris. PLoS ONE, 2011 6:e21430. doi:10.1371/journal.pone.0021430.

• Hirakawa Y, Jimbo R, Shibata Y, Watanabe I, Wennerberg A, Sawase T. Accelerated bone forma-tion on photo-induced hydrophilic titanium implants: an experimental study in the dog mandi-ble. Clin Oral Impl Res, In Press

• Hu L.H., Farrokhnia M., Heimdal J., Shleev S., Rulisek L., Ryde U. Reorganization energy for internal electron transfer in multicopper oxidases. Journal of Physical Chemistry B, 2011, 115(45), 13111-13126.

• Ivan Alajbeg; Denise P Falcão, Simon D Tran, Rafael A Martín-Granizo, Gloria I Lafaurie, Gloria R Bautista Mendoza, Slavica Pejda, Lea Vuletić, Rubén Mantilla; Soraya C Leal, Ana C Barreto Bezerra, Henri A Ménard, Suguru Kimoto, Shaoxia Pan, María L Maniegas Lozano, Cheryl A Krushinski, Dario Melilli, Giuseppina Campisi, Carlo Paderni; Domenica Matranga, Juan F Yepes, Liselott Lindh, Meltem Koray, Gonca Mumcu, Sharon Elad, Itai Zeevi, Beatriz C Aldape, Rodrigo M López Sánchez, Claire L Lassauzay, Olivier Fromentin, Ben Z Beiski, Franz P Strietzel, Yrjö T Konttinen, Andy Wolff, Susan L Zunt., Intraoral Electrostimulator for Xeros-tomia Relief: A Long-term, Multicenter, Open-label Clinical Trial. Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology and Endodontology Accepted 2012-01-03.

• J. Li, C. Dong, P. Jönsson, and G. Gaigalas, MF -dependent Hyperfine Induced Transition Rates in an External Magnetic Field for Be-like 47Ti18+

, Physics Letters A 375 914 (2011).

• Jimbo R, Coelho PG, Vandeweghe S, Schwartz-Filho HO, Hayashi M, Ono D, Andersson M, Wennerberg A. Histological and three-dimensional evaluation of osseointegration to nano-structured calcium phosphate-coated implants. Acta Biomater, Accepted for Publication

• Jimbo R, Rodriguez-Fernandez J, Sul YT, Johansson CB. Principal component analysis: A novel analysis to evaluate the characteristics of osseointegration on different implant surfaces. Implant Dent, Accepted for Publication

• Jimbo R, Xue Y, Hayashi M, Schwartz HO, Andersson M, Mustafa K, Wennerberg A. Gene expression and biomechanical analysis of nano-structured calcium phosphate implants. J Dent Res, Accepted for publication

• Jimbo R., Sotres J., Johansson C., Breding K., Currie F., Wennerberg A. The biological response to three different nanostructures applied on smooth implant surfaces. 2011. Clinical Oral Implants Research. doi:10.1111/j.1600-0501.2011.02182.x

• Johansson CB, Gretzer C, Jimbo R, Mattisson I, Ahlberg E. Enhanced implant integration with hierarchically nano-structured implants; a pilot study in rabbits. Clin Oral Impl Res, Accepted for Publication

• Ketelhuth DFJ, Rios FJO, Wang Y, Liu H, Johansson ME, Fredrikson GN, Hedin U, Gidlund


46

M, Nilsson J, Hansson GK, Yan Z-Q. Identification of a danger-associated peptide from apoli-poprotein B100 (ApoBDS-1) that triggers innate pro-atherogenic responses. Circulation, Nov 29;124(22):2433-43, 2011.

• Kocherbitov, V.; Alfredsson, V. Assessment of Porosities of SBA-15 and MCM-41 Using Water Sorption Calorimetry. Langmuir (2011), 27, 3889-3897.

• Kolbus D, Wigren M, Ljungcrantz I, Söderberg I, Björkbacka H, Nilsson J and Fredrikson GN. Immunization with cationized BSA inhibits progression of disease in Apobec-1/LDL receptor deficient mice with manifest atherosclerosis. Immunobiology, 216:663-669, 2011.

• Lavant, E., Agardh, D., Nilsson, A., & Carlson, J. (2011). A new PCR-SSP method for HLA DR-DQ risk assessment for celiac disease. Clinica chimica acta; international journal of clinical chemistry

• Leisnert L, Karlsson M, Franklin I, Lindh L, Wretlind K., Improving teamwork between students from two professional programmes in dental education. Eur J Dent Educ. 16: 17-26, 2012

• Neilands J, Petersson LG, Beighton D and Svensäter G. Fluoride-supplemented milk inhibits acid tolerance in root surface biofilms. Caries Res 2011, Accepted for Publication

• Neilands J, Sutherland D, Resin A, Wejse PL and Chávez de Paz LE. Chitosan nanoparticles affect the acid tolerance response in adhered cells of Streptococcus mutans. Caries Res 2011, 45:501-505.

• Ohlsson B, Sjöberg K, Alm R, Fredrikson GN. Patients with irritable bowel syndrome (IBS) and dysmotility express antibodies against gonadotropin realising hormone (GnRH) in serum. Neurogastroenterol Motil, Nov; 23(11):1000-6, e459, 2011.

• Olof Svensson and Thomas Arnebrant, Antibody-Antigen interaction at polystyrene: An in situ ellipsometric study, Journal of Colloid and Interface Science, in press 2011, doi: 10.1016/j.jcis.2011.11.034

• Ono D, Jimbo R, Kawachi G, Ioku K, Ikeda T, Sawase T. Lateral bone augmentation with a newly developed beta-tri-calcium phosphate block: an experimental study in the rabbit mandi-ble. Clin Oral Impl Res; 2011, Available on early view

• P. Idelicato, J. Bieron, and P. Jönsson, Are MCDF calculations 101 % correct in the superheavy elements range?, Theoretical Chemistry Accounts: Theory, Computation, and Modeling (Theo-retica Chimica Acta) 129, Numbers 3-5, 495 (2011).

• P. Jönsson, P. Rynkun, and G. Gaigalas, Energies, Transition Rates, Hyperfine Structures, and Landé gJ Factors for the Fine-structure Levels of the 2s2 2p2, 2s2 p2, and 2p4 Configurations in Carbon-like Ions between F IV and Ni XXIII, Atomic Data and Nuclear Data Tables, 97 648 (2011).

• Penkova, A.; Polotskaya, G.; Toikka, A.; Kocherbitov, V. Effect of residual solvent on physico-chemical properties of poly(phenylene isophtalamide) membrane. Drying Technology (2011), 29, 633-641.

• Pihl M, Davies JR, Johansson AC and Svensäter G. Bacteria on catheters from patients undergo-ing peritoneal dialysis. Peritoneal Dialysis Int 2011, Accepted for Publication


47

• Pita M., Gutierrez-Sanchez C., Olea D., Velez M., Garcia-Diego C., Shleev S., Fernandez V.M., De Lacey A.L. High redox potential cathode based on laccase covalently attached to gold elec-trode. Journal of Physical Chemistry C, 2011, 115(27), 13420-13428.

• Reichhardt, N.; Nylander, T.; Klösgen, B.; Alfredsson, V.; Kocherbitov, V. Porosity and surface properties of SBA-15 with grafted PNIPAAM: A water sorption calorimetry study. Langmuir (2011), 27, 13838–13846.

• Santos, O., Lindh, L., Halthur, T., Arnebrant, T., Adsorption from saliva to silica and hydroxya-patite surfaces and elution of salivary films by SDS and delmopinol. Biofouling virtual special issue 2011

• Santos, O, Svendsen, IE, Lindh, L, Arnebrant, T, Adsorption of HSA, IgG and laminin-1 on model titania surfaces – effects of glow discharge treatment on competitively adsorbed film composition Biofouling, 27: 1003-1015, 2011

• Senadheera DB, Cordova M, Ayala EA, Chávez de Paz LE, Singh K, Downey JS, Svensäter G, Goodman SD and Cvitcovitch D. Regulation of bacteriocin production and cell death by the VicRK signaling system in Streptococcus mutans. J. Bact 2011, Accepted for Publicatio

• Simonpieri A, Del Corso M, Vervelle A, Jimbo R, Inchingolo F, Sammartino G, Dohan Ehrenfest DM. Current knowledge and perspectives for the use of Platelet-Rich Plasma (PRP) and Platelet-Rich Fibrin (PRF) in oral and maxillofacial surgery. Part 2: bone graft, implant and reconstruc-tive surgery. Current Pharmaceutical Biotechnology, Accepted for Publication

• Singh R. N, Viswanathan U K, Kumar Sunil, et al. Influence of hydrogen content on impact toughness of Zr-2.5Nb pressure tube alloy. NUCLEAR ENGINEERING AND DESIGN Volume: 241 Issue: 7 Pages: 2425-2436

• Sonesson M, Ericson D, Kinnby B and Wickström C. Glycoprotein 340 and sialic acid in minor-gland and whole saliva of children, adolescents and adults. Eur J Oral Sci 2011, 119:435-440.

• Sonestedt E, Wirfält E, Wallström P, Gullberg B, Drake I, Hlebowicz J, Nordin Fredrikson G, Hedblad B, Nilsson J, Krauss R, and Orhu-Melander M. High disaccharide intake associates with atherogenic lipoprotein. Br J Nutr, 2011 Oct 20:1-8. [Epub ahead of print]

• Sotres J., Barrantes A., Arnebrant T. Friction Force Spectroscopy as a Tool to Study the Strength and Lateral Diffusion of Protein Layers. 2011. Langmuir. 27: 9439-9448

• Sotres J., Svensson O., Arnebrant T. Friction force spectroscopy of β- and κ-casein monolayers. 2011. Langmuir. 27: 981-992.

• Sotres, J, Lindh, L, Arnebrant, T, Friction and force measurements on HWS and influence of pH changes on film stability. Langmuir, 27:13692-700, 2011, doi:10.1021/la202870c

• Stollenwerk M, Svensson O, Schiopu A, Jansson B, Arnebrant T, and Fredrikson GN. Adsorption of low density lipoprotein, its oxidation and subsequent binding of specific recombinant antibod-ies – an in situ ellipsometric study. Biochimica et Biophysica Acta, Feb;1810(2):211-7, 2011.

• Strietzel FP, Lafaurie GI, Bautista Mendoza GR, Alajbeg I, Pejda S, Vuletić L, Mantilla R, Falcão DP, Leal SC, Barreto Bezerra AC, Tran SD, Ménard HA, Kimoto S, Pan S, Martín-Granizo RA, Maniegas Lozano ML, Zunt SL, Krushinski CA, Melilli D, Campisi G, Paderni C, Dolce S, Yepes JF, Lindh L, Koray M, Mumcu G, Elad S, Zeevi I, Aldape Barrios BC, López Sánchez RM, Beiski BZ, Wolff A, Konttinen YT. Efficacy and safety of an intraoral electrostimulation device for


48

xerostomia relief: a multicenter randomized trial. Arthritis & Reumatism. 63: 180-190, 2011, DOI: 10.1002/art.27766

• Sumi T, Braian M, Shimada A, Shibata N, Takeshita K, Vandeweghe S, Coelho PG, Wennerberg A, Jimbo R. Characteristics of implant-CAD/CAM abutment connections of 2 different internal connection systems. J Oral Rehab, Accepted for Publication

• Svendsen, IE, Santos, O, Sotres, J, Breding, K, Wennerberg, A, Arnebrant, T, Lindh, L, Adsorption of HSA, IgG and laminin-1 on model hydroxyapatite surfaces – effects of surface characteristics Biofouling, 28: 87-97, 2012

• Svendsen, IE., Lindh, L. The composition of enamel salivary films is different from the ones formed on dental materials. Biofouling virtual special issue 2011

• Valverde GB, Jimbo R, Teixeira HS, Bonfante EA, Janal MJ, Coelho PG. Evaluation of surface roughness as a function of multiple blasting processing variables. Clin Oral Implants Res, In Press

• Wang X., Falk M., Ortiz R., Matsumura H., Bobacka J., Ludwig R., Bergelin M., Gorton L., Shleev S. Mediatorless sugar/oxygen enzymatic fuel cells based on gold nanoparticle-modified electrodes. Biosensors and Bioelectronics, 2012, 31(1), 219-225.

• Wennerberg A, Jimbo R, Allard S, Skarner G, Andersson M. In vivo Stability of hydroxyapatite nano-meter particles coated on titanium implant surfaces. Int J Oral Maxillofac Implants. 2011 Nov-Dec; 26(6): 1161-6

• Vidakovic-Koch, T, Ivanov I, Falk M, Shleev S, Ruzgas T, Sundmacher K, Impact of the gold support on the electrocatalytic oxidation of sugars at enzyme-modified electrodes. Electroanaly-sis, 2011, 23(4), 927-930.

• Wigren M, Kolbus D, Dunér P, Ljungcrantz I, Söderberg I, Björkbacka H, Fredrikson GN, Nilsson J. Evidence for a role of regulatory T cells in mediating the athero-protective effect of apolipoprotein B peptide vaccine. J Int Med, May 269(5):546-56, 2011.

• Albrektsson T, Wennerberg A. The science of osseointegration. In; Prosthodontic Treatment for Edentulous Patients, ed 13. Submitted.

• Alm K, Cirenajwis H, Gisselsson L, Gjörloff Wingren A, Janicke B, Mölder A, Oredsson S, Persson J. Digital holography and cell studies. Holography, Research and Technologies, In Tech, edited by Joseph Rosen February 2011. ISBN 978-953-307-227-2

• Blum, Z. and Eriksson, H. Dealuminated Zeolites in Biological Systems, in; Zeolites: Synthesis, Chemistry and Applications. Editors: M. K. Andreyev and O. L. Zubkov, Nova Science Publish-ers, Inc., N Y. In press 4.

• Davies, J.R., Wickström, C. and Thornton, D. J. Gel-forming and cell-associated mucins – prepa-ration for structural and functional studies. In: Mucins: Methods and Protocols, Methods in Molecular Biology, vol. 842, Eds: McGuckin MA and Thornton DJ DOI 10.1007/978-1-61779-513-8_2, © Springer Science+Business Media, LLC. Accepted for Publication

• Gotfredsen K, Wennerberg A, Gunne J. Implants and Their Role in FDP Treatment. Fixed Pros-thodontics eds Nilner, Karlsson, Dahl. Submitted

BOOKS AND BOOK CHAPTERS 2.


49

• Jimbo R. Abutment selection, screw or cementation? Prosthetic Practice, 2011 (Japanese)

• Johansson CB, Jimbo R, Rosen K. Histological analysis. Comprehensive biomaterials. Elsevier 2011

• Ono D, Jimbo R, Sawase T. Impression techniques in implant prosthetics. Prosthetic Practice, 2011 (Japanese)

• Wennerberg A, Rosén B-G, Albrektsson T. Surface topography and measuring techniques for dental implant applications. -Possibilities and obstacles In Press Implant Dentistry Research Guide: Basic, Translational and Experimental Clinical Research. Nova Science Publishers

• Winning T, Skinner V, Kinnell A, Townsend G, Svensäter G, Rohlin M and Davies J. The Influ-ence of Two PBL Curricula Contexts on First-year Students’ Understandings of PBL, Approaches to Learning and Outcomes; In: Problem-Based Learning in Clinical Education. Eds: Bridges S; McGrath C and Whitehill, T L. Springer Science + Business Media LLC, Accepted for Publication

• F Hasse, N Tibbelin and JR Davies. Protease activity in Lactobacillus salivarius and Actinomy-ces naeslundii in a model of root canal infection. Swed Dental J 2011, 35:226.

• H Fransson, K Petersson and JR Davies. Influence of bacterial products on the viability of odontoblast–like cells. Swed Dental J 2011, 35:227.

• Wennerberg A. Em Foco, Entrvista. Jornal Ilapedo 2011 vol 5, no 3. Brazil.

POPULAR ARTICLES3.


50

• Prof. Lars Wadsö, Lund Univeristy, Sorption calorimetry as a tool to study hydration, 2011-04-01

• Gediminas Niaura, Vibrational spectroscopy for hydration studies, 2011-04-01

• Audrius Misiunas, Jekaterina Latynis, Spectroscopic studies of hydration of lysozyme and cyto-chrome c, 2011-04-01

• Dr. Justas Barauskas, Hydration of lipid-based drug delivery systems, 2011-04-01

• Prof. Håkan Wennerström Interlamellar forces and the thermodynamic characterization of lamellar phospholipid systems, 2011-04-01

• Prof. Alexander Toikka, Department of Chemical Thermodynamics and Kinetics, Saint-Peters-burg University, Application of thermodynamic methods to the membrane processes: descrip-tion and modeling of pervaporation, 2011-04-01

• Prof. Irina Zvereva, Department of Chemical Thermodynamics and Kinetics, Saint-Petersburg University, Processes of hydration and cation exchange in layered oxide structures, 2011-04-01

• Sergei Gavrushov, Dielectric model of hydration shells of metal ions: an impact on electrostatics of DNA/RNA, 2011-04-01

• Prof. György Marko-Varga, Dept. of Measurement Technology and Industrial Electrical Engi-neering , Lund University, Clinical Analysis in Lung Cancer and COPD Studies, 2011-06-14

• Dr. Javier Sotres, Health and Society, Malmö University, Friction Force Spectroscopy of Protein Layers: Strength and Lateral Diffusion Studies, 2011-06-14

• Dr. Maria Pihl, Institutionen för kemi- och bioteknik, Chalmers, Microbial biofilms on perito-neal dialysis catheters, 2011-06-14

• Prof. Erik V Thomsen, DTU Nanotech, Technical University of Denmark, Sensors Everywhere: An Electronic Patch, 2011-10-24

• Malin Prydz, Product Designer, Mölnlycke Health Care AB, Wound Dressings - From Product Design Perspective, 2011-10-24

• Dr. Maria Wertén, Mölnlycke Health Care AB, In Vitro Testing of Bioactive Wound Dressings During Product Development, 2011-10-25

• John McCormack, MiniFAB, Nanolitre Tear Sampling in Diagnostics: Concept to Commercial Product, 2011-10-25

• Assoc. Prof. Sergey Shleev, Health and Society, Malmö University, Operation of Microscale Electrochemical (Bio)Devices in Human Lacrimal Liquid (Tears) , 2011-10-25

• Prof. Enno Veerman, Department Oral Biochemistry, Academic Centre Dentistry Amsterdam, Saliva-based Diagnostics: A Critical Appraisa, 2011-10-25

• Dr. Javier Sotres, Health and Society, Malmö University, Friction Force Spectroscopy as a Tool to Study Salivary Films, 2011-10-25

• Prof . Thomas Arnebrant, Health and Society, Malmö University, Interfacial Behavior of Salivary Molecules – Relevance to Diagnostics, Sensing and Device Construction, 2011-10-25

VISITORS AND SEMINARS AT THE CENTER


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• Assoc. Prof. Marie Lodén and Ulf Åkerström, Skin barrier function and humectants in moistur-izers, 2011-11-15

• Dr. Per Mercke, Immaterial property rights, 2011-12-08

• Laura Adamonyte, Master student, Vilnius University, 2 months. Phase studies of synthetic lipids (Erasmus scholarship)

• Marija Jankunec, PhD student, Vilnuis University, 3 months. Studies of hydration and phase behavior of lipids. Title of presentation: “Hydration of lipid systems. DOPC”, 2011-01-18. (Swedish institute Visby program)

• Jekaterina Latynis, PhD student, Vilnuis University, 3 months. Studies of hydration of proteins. Title or presentation: “Spectroscopic studies of hydration of lysozyme and cytochrome c”, 2011-04.01. (Swedish institute Visby program)

• Anastasia Penkova, Post-doc, St.Petersburg University, 1 month. Studies of diffusion of model compounds in biopolimers. (Swedish institute Visby program)

• Mikhail Chislov, PhD student, St.Petersburg University, 1 month. Experiments on hydration of layered oxides. (Swedish institute Visby program)

• Anastasiia Venediktova, PhD student, St.Petersburg University, 1 month. Experimental studies of phase behavior of surfactants. (Swedish institute Visby program)

• Dr. Karin Bergström, AkzoNobel, Sweden, 2011-02-14 – 15

• Dr. Christy Whiddon, AkzoNobel, Sweden, 2011-02-14 – 15

• Dr. Peter Westbye, AkzoNobel, Sweden, 2011-02-14 – 15

• Prof. Peter Bauer, Bayer Crop Science, Germany, 2011-02-14 – 15

• Dr. Shuaib A Khan, AkzoNobel, NY, USA, 2011-02-14 – 15

• Dr. Dmitrii Pankratov, a guest researcher from Institute of Biochemistry, Russian Academy of Sciences, Moscow, Russia, 2011-07-13 – 09-09

• Mr. Paul Santner, Master student of Biotechnology from BOKU University, Vienna, Austria, 2011-09-12 – 12-30

• Marius Dagys, PhD at Vilnius University, Vilnius, Lithuania. 2011-04-04 – 05-27.

• Vida Krikstolaityte, PhD at Vilnius University, Vilnius, Lithuania. 2011-09-01 – 2012-08-31

• Laura Abariute, Erasmus supported master student, Vilnius Gediminas Technical University, Vilnius, Lithuania. 2011-06-01 – 08-30

• Tetsuro Odatsu, 2 months

• Daisuke Ono, 10 days

• Kenji Miyahara, 10 days

• Ryota Muratomi, 10 days

• Koutaru Tsuiki, 10 days

• Kazuhiro Nakashima, 10 days

• Humberto Scwartz, 3 months

• Anders Zachrisson


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• Prof. Jeannine Brady, University of Florida, USA

• Prof. Gunnar Bergenholtz, University of Gothenburg

• Assoc. Prof. Anette Carlén, Gothenburg University

• Assoc. Prof. Marie Skepö, Lund University

• Assoc. Prof. Tim Tolker-Nielsen, University of Copenhagen

• Dr. Peter Weise, ArlaFoods

• Dr. Jan Hall, Nobel Biocare

• Dr. Helena Jeppsson, Gambro

• Dr. Anna Westerlund, Gothenburg University

• Dr. Torgny Sjödin, Sinclair Pharma

• Anthony P F Turner, Center for Biosensors and Bioelectronics, Linköping University, “Biosen-sors: meeting the demand for personal diagnostics”, 2011-02

• Debby Chang , Department of Physical Chemistry, Lund University, “Lubrication of biological joints”, 2011-02

• Sven Scholander, STP Kristiandstad, ”Singel implantats”, 2011-02

• Stefan Vandeweghe, Prosthetic Dentistry, Mah, “Factors for implant survival” , 2011-03

• Rolf Liedholm, Oral Surgery, and Liselott Lindh, Prosthetic Dentistry, Mah, “Dry mouth signs and treatments” , 2011-03

• Yvone Ceder, Department of Clinical Chemistry, Lund University, “MicroRNAs in prostate cancer”, 2011-03

• Henrietta Nielsen, Dept of Clinical Sci Malmö, Molecular Memory Research Unit, The Wallen-berg Lab, Skåne University Hospital Malmö, 2011-03

• Maria Pihl, Mah, “Biofilms on peritoneal dialysis catheters.”, 2011-03

• Marjan Dorkhan Mah, “Biofilms on titanium implant surfaces”, 2011-03

• Ingrid Collin-Bagewitz, STP Malmö and Kerstin Petersson, Endodontics, Mah, “National guide-lines in dentistry” , 2011-03

• Anders Sundh, Umeå University, “Zirconia in odontology” , 2011-04

• Eddie Thordarson, Magle Life Science AB, Kristianstad/Lund, “Biodegradable microspheres as medical devices”, 2011-04

• Maria Stollenwerk, Biomedical Laboratory Science / Technology, Malmö University, “Adsorp-tion of low-density lipoprotein, its oxidation, and subsequent binding of specific recombinant antibodies: An in situ ellipsometric study.”, 2011-05

• Henrietta Nielsen, Dept of Clinical Sci Malmö, Molecular Memory Research Unit, The Wallen-berg Lab, Skåne University Hospital Malmö, “The glial spect of neurodegenerative dementia”, 2011-05

• Olle Melander, Lund University Diabetes Centre & Skåne University Hospital in Malmö, “Biomarkers of cardiovascular diseases 2011-06

INTERNAL SEMINARS AT THE CENTER


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• Liv Kroona, Mah ”Lichenoid changes and Oral Biofilms”, 2011-06

• Lars Bondemark ” Nursing Research - Status and Future”, 2011-09

• Aje Carlbom, HS ” Qualitative methods - a general overview” , 2011-10

• Magnus Englander, HS ” Phenomenological Research Method”, 2011-10

• Andreas Pettersson & Krister Nilner, Prosthetic dentistry, Mah, “CAD/CAM-guided dental implant surgery” , 2011-10

• Magnus Falk, Mah, “Design and performance of biofuel cells”, 2011-11

• Peter Billsten, Mah, “Pharmaceutical applications of Raman spectroscopy and imaging”, 2011-11

• Mats Trulsson, KI, Stockholm, “Oral motor activity”, 2011-11

• Maria Alvarado-Kristensson, Department of Pathology, Lund University, Malmö, “Regulators of the restriction point and cancer”, 2011-12

• Cathrine Albér, Mah, “Penetration of model drug compounds through skin”, 2011-12


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CONTRIBUTIONS TO AND PARTICIPATION INCONFERENCES AND WORKSHOPS

ORAL PRESENTATIONS 2011

INTERNATIONAL• Björklund S, A water gradient can be used to regulate biophysical properties of skin, 2nd Skin Trail Blazer Workshop, Embassy Suites Boston Massachusetts Institute of Technology (USA) 5-6 Aug, 2011

• Davies JR and Bondemark L. Research Education at The Faculty of Odontology, Malmö University. University of Copenhagen, 2011. Invited speaker.

• Dorkhan M, Chavéz de Paz, Svensäter G and Davies JR. Effect of saliva and serum pellicles on adhesion of Streptococcus oralis to titanium surfaces. 10th European Oral Microbiology Work-shop, Zeist, The Netherlands, 2011.

• Jimbo R. Analysing modern implants and its clinical significance. Osseospeed Introductory meeting, 2011

• Jimbo R. Effect of nanostructures on osseointegration and new evaluation approaches. Nano-technology in Odontology, 2011

• Jimbo R. State of the art implant research topics and future paths, 2011

• Neilands J, Davies JR and Svensäter G. The effect of Lactobacillus strains on Streptococcus mutans acid adaptation. 10th European Oral Microbiology Workshop, Zeist, The Netherlands, 2011

• P. Jönsson, S. Gustafsson, J. Ekman, P. Bengtsson, P. Rynkun, and G. Gaigalas, CI calculations, 43st European Group for Atomic Systems, Fribourg (Switzerland), 28 Jun – 2 Jul, 2011

• Peter Lamberg, Marius Dagys, Javier Sotres, Thomas Arnebrant and Tautgirdas Ruzgas, Comparison of techniques for determination of nanoparticle diameter. 5th SMCBS’2011 Work-shop at Łochów, Poland, 4-8 Apr, 2011

• S. Verdebout, J.G. Li, C. Naze, M. Godefroid, P. Jönsson, and G. Gaigalas, Atomic properties of neutral Ne using the LCFI method, 43st European Group for Atomic Systems, Fribourg (Swit-zerland), 28 Jun – 2 Jul, 2011

• S. Verdebout, M. Godefroid, P. Rynkun, P. Jönsson, G. Gaigalas, and C. Froese Fischer, Inter-action of Variational Localized Correlation Functions for Atomic Properties, 43st European Group for Atomic Systems, Fribourg (Switzerland), 28 Jun – 2 Jul, 2011

• S. Verdebout, P. Rynkun, P. Jönsson, G. Gaigalas, C. Froese Fischer and M. Godefroid, Interac-tion of Variational Localized Correlation Functions for Atomic Properties of Be I, XXVII Inter-national Conference on Photonic, Electronic and Atomic Collisions Belfast, (Northern Ireland, UK), 27 Jul – 2 Aug, 2011

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• Schwartz-Filho HO, Wennerberg A, Jimbo R, Ramos-Junior ES, Morandini ACF, Marcantonio RAC, Marcantonio E Jr, Santos CF. Cytokine production in gingival fibroblasts cultured on distinct titanium surfaces. Latin American Osseointegration Congress, 2011

• Shleev S., Invited Lecture entitled: “Mediatorless sugar/oxygen enzymatic fuel cells based on gold nanoparticle-modified electrodes” at the 62st Annual Meeting of ISE, Niigata, Japan

• Shleev S., Invited Lecture entitled: “Miniaturized enzymatic fuel cells” at 2011 Workshop on Biomimetic and Bioanalytical Systems, Luckenwalde, Germany

• Shleev S., Invited Lecture entitled: “Nanostructured surfaces for biofuel cell application” at 5th Nanoschool, Vilnius, Lithuania

• Shleev S., Invited Lecture entitled: “Nanostructured surfaces for biofuel cells” at Creative Nano, Taastrup, Denmark

• Shleev S., Invited Lecture entitled: “Potentially implantable bioelectronic devices for biosensing and biofuel cell applications” at SMCBS’2011 International Workshop, Lochόw, Poland

• Shleev S., Invited Lecture entitled: “Three-dimensional nanobiostructure-based self-contained biodevicesfor biomedical application” at Update 2011, Helsingborg, Sweden

• Svensäter G. Control of Bacterial Function in Relation to Disease. 2nd European Congress on Microbial Biofilms, Copenhagen, Denmark, 2011. Invited speaker.

• Svensäter G. Infection control on the biofilm level. Scandinavian Society for Biomaterials 4th Annual Meeting, Fiskebäckskil, Sweden, 2011. Invited speaker.

• Wennerberg A. Biological and mechanical responses to implants, abutments and supraconstruc-tions. 2011 International conference, Southern Implants, 9-11 May 2011 Chobe, Botswana. Invited lecture.

• Wennerberg A. Finding optimal solutions for implant surfaces. Seminars of Turku Clinical Biomaterial Center TCBC, Turku, Finland, 1 Mar 2011. Invited lecture.

• Wennerberg A. Implant development. Past, present and future of implant surfaces. The 8th Annual MEGAGEN international symposium. Milan Italy, 11-12 Nov, 2011. Invited lecture.

• Wennerberg A. Implant surfaces and the importance for soft and bone tissue integration, 4 hour international course, In 2011, Latin American Osseointegration Congress, 2-5 July 2011. Invited lecture.

• Wennerberg A. Implant surfaces, In 2011, Latin American Osseointegration Congress, 2-5 July 2011. Invited lecture.

• Wennerberg A. Nanostructures for tissue adhesion. Pre conference. P-I Implants. In 2011, Latin American Osseointegration Congress, 2-5 July 2011. Invited lecture.

• Wennerberg A. Role of surface characterisation and improving osseointegration and related advancement. The Indian Society of Oral Implantologists. 21-23 Oct 2011, Hyderabad, Indien. Invited lecture.

• Wickström C, Chávez de Paz LE, Kindblom CA, Jonsson S and Svensäter G. Using salivary mucin MUC5B as a surface-associated conditioning film for bacterial studies. 9th European Symposium on Saliva, Egmond aan Zee, Netherlands, 2011. Invited speaker.


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• Fransson H, Petersson K and Davies JR. Influence of bacterial products on the viability of odontoblast–like cells. 47th Annual Congress of the Swedish Society, Stockholm, 2011

• Hasse F, Tibbelin N and Davies JR. Protease activity in Lactobacillus salivarius and Actinomy-ces naeslundii in a model of root canal infection. 47th Annual Congress of the Swedish Society, Stockholm, 2011

• Lindh L., “ Oral hygiene and dry mouth”, Mun- & Halscancerförbundets rehabiliteringskurs, Höör, 2011

• Svensäter G, Neilands J, Wickström C, Kinnby B and Davies JR. Infection Control & Biomark-ers on the biofilm level. Imego, Gothenburg, 2011

• Svensäter G, Neilands J, Wickström C, Kinnby B and Davies JR. Infection Control & Biomark-ers on the biofilm level. Brånemark Clinic, Gothenburg, 2011

NATIONAL

LOCAL• Dorkhan M. Activities of microbial biofilms on bioactive implant surfaces. PhD student work-shop, Faculty of Odontology Malmö University, 2011

• Javier Sotres, Health and Society, Malmö University, Friction Force Spectroscopy as a Tool to Study Salivary Films, 2011-10-25

• Javier Sotres, Health and Society, Malmö University, Friction Force Spectroscopy of Protein Layers: Strength and Lateral Diffusion Studies, 2011-06-14

• Johan Engblom, Health and Society, Malmö University, Hydration dependence of barrier func-tion of biomembranes, 2011-04-01

• Johan Engblom, Health and Society, Malmö University, Responding Biological Membranes - Skin and Mucosa, 2011-10-25

• Maria Pihl, Institutionen för kemi- och bioteknik, Chalmers, Microbial biofilms on peritoneal dialysis catheters, 2011-06-14

• Sergey Shleev, Health and Society, Malmö University, Operation of Microscale Electrochemical (Bio)Devices in Human Lacrimal Liquid (Tears) , 2011-10-25

• Tautgirdas Ruzgas, Health and Society, Malmö University, Characterisation of Biological Membranes by Impedance Spectroscopy, 2011-10-24

• Thomas Arnebrant, Health and Society, Malmö University, Interfacial Behavior of Salivary Molecules – Relevance to Diagnostics, Sensing and Device Construction, 2011-10-25

• Vitaly Kocherbitov, Health and Society, Malmö University, Driving forces of hydration of surfactants, 2011-04-01

• Vitaly Kocherbitov, Health and Society, Malmö University, Thermodynamics of hydration of proteins, 2011-04-01


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POSTERS 2011

INTERNATIONAL • Ahlgren C, Björnsson L, Diogo-Löfgren C, Fransson, H, Jansson H, Johansson V, Lager A, Ljunggren A, Neilands J, Papias E, Petrén S, Sonesson M and Wickström C. The Malmö Model: How to Achieve Future Sustainability in a Problem-Based Curriculum. 7th International Conference on PBL in Dentistry, Whistler, Canada, 2011

• Björklund, S, A water gradient can be used to regulate biophysical properties of skin, Barrier Function of Mammalian Skin, Waterville Valley Resort in Waterville Valley NH (USA), 7-12 Aug, 2011

• Bougas K, Franke-Stenport V, Currie F, Wennerberg A. Laminin is highly reactive with titanium surfaces in vitro. IADR/AADR/CADR San Diego, Calif., USA, 16-19March, 2011

• Chávez de Paz LE, Svensäter G and Wickström C. Compositional changes during biofilm formation on human salivary MUC5B conditioning film. 2nd European Congress on Microbial Biofilms, Copenhagen, Denmark, 2011

• Davies JR, Rohlin M and Svensäter G. The Oral Ecosystem as a Model for Dental Education and Research. 7th International Conference on PBL in Dentistry, Whistler, Canada, 2011

• Dorkhan M, Chavez de Paz LE, Svensäter G and Davies JR. Effect of surface roughness on adhesion of Streptococcus oralis to titanium surfaces. 2nd European Congress on Microbial Biofilms, Copenhagen, Denmark, 2011

• Falkman P, Pedersen L, Engblom J, Cubic phases in the DOPS/POPE/water system;Geometry at Interfaces, Primosten (Croatia), 3-7 Oct, 2011

• I.E. Svendsen, T. Arnebrant, L. Lindh, Effect of low pH rinsing on in situ formed salivary films. 9th European Symposium on Saliva. Saliva Symposium. 22-25 May, 2011 Egmond aan Zee, the Netherlands

• Jimbo R, Coelho PG, Schwartz-Filho HO, Xue Y, Mustafa K, Andersson M, Wennerberg A. Osseointegration enhanced by nano-structured CaP coated implants: a molecular, biomechanical and μCT analysis. European Association for Osseointegration, 2011

• Neilands J, Sutherland D, Resin A, Weise PL, Chavez de Paz LE. Chitosan nanoparticles affect the acid tolerance response in Streptococcus mutans biofilms. 2nd European Congress on Microbial Biofilms, Copenhagen, Denmark, 2011

• Pihl M, Svensäter G and Davies JR. Effects of extracellular products from Pseudomonas aeruginosa on Staphylococcus epidermidis biofilms on peritoneal dialysis catheters. 2nd European Congress on Microbial Biofilms, Copenhagen, Denmark, 2011

• Svensson B, Nieto C, Delfani P, Chia J, Gjörloff Wingren A, Ruzgas T, Engblom J, A modern hydrogen peroxide cream for wound healing, AAD, New Orleans (USA) 2011

• Svensson, B, Wingren, AG, Abad, CN, Chia, J, Delfani, P, A modern hydrogen peroxide cream for wound healing, 69th Annual Meeting of the American Academy of Dermatology, New Orleans, LA (USA) 4-8 Feb, 2011

• V. Krikstolaityte, A. Barrantes, T. Arnebrant, A. Ramanavicius, T. Ruzgas. Influence of ionic strength on physicochemical properties of gold nanoparticle multilayer structures. 13th International conference-school on “Advanced materials and technologies”, 27-31 August, 2011, Palanga, Lithuania

• Winning TA, Kinella A, Skinner V, Townsend G, Svensäter G, Rohlin M, Davies JR. First- and third-year students’ approaches to learning in two PBL dental curricula. 7th International

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Conference on PBL in Dentistry, Whistler, Canada, 2011

• Winning TA, Skinner V, Townsend G, Svensäter G, Rohlin M, Davies JR. The First- and third- year students’ understandings of PBL from two dental curricula. 7th International Conference on PBL in Dentistry, Whistler, Canada, 2011

• Albér C, Lodén M, Kocherbitov V, “The effect of topical hyaluronic acid on skin permeability and moisturization”, Soft and Hard Materials, Lund (Sweden), 25-27 Oct, 2011

• Dorkhan M, Svensäter G and Davies JR. “Adhesion of Streptococcus oralis to titanium surfaces- effects of surface roughness and conditioning films.” Workshop on development and regeneration of bones and teeth. Umeå (Sweden), 2011

• Albér C, Lodén M, Kocherbitov V, “The effect of topical hyaluronic acid on skin permeability and moisturization”, Soft and Hard Materials, Lund (Sweden), 25-27 Oct, 2011

• Alejandro Barrantes, Olga Santos and Thomas Arnebrant, “Viscoelastic Properties of Poly-L-Lysine/Heparin Multilayers”, Biofilms 7th Annual workshop, Malmö, 24-25 Oct, 2011

• Björklund, S, “A water gradient can be used to regulate biophysical properties of skin”, Biofilms 7th Annual workshop, Malmö, 24-25 Oct, 2011

• El-Schich, Z, Mölder, A, Tassidis, H, Sebesta, M, Lenart, T, Gustafsson, M , Gisselsson, L, Alm, K, Härkönen, P, Gjörloff Wingren A, “Using digital holography for measurements of growth, viability and death of adherent cells”, Biofilms 7th Annual workshop, Malmö, 24-25 Oct, 2011

• Falkman P, Pedersen L, Engblom J, “Cubic phases in the DOPS/POPE/water system”, Biofilms 7th Annual workshop, Malmö, 24-25 Oct, 2011

• I.E. Svendsen, T. Arnebrant and L. Lindh, “Effect of low pH rinsing on in situ formed salivary films”, Biofilms 7th Annual workshop, Malmö, 24-25 Oct, 2011

• Javier Sotres, Alejandro Barrantes, Olof Svensson, Liselott Lindh, and Thomas Arnebrant, “Friction Force Spectroscopy as a Tool to Study the Strength, Lateral Diffusion and Structure of Proteinaceous Layers”, Biofilms 7th Annual workshop, Malmö, 24-25 Oct, 2011

• Jekaterina Latynis, Justas Barauskas and Gediminas Niaura, “Hydration of Cytochrome C studied by vibrational spectroscopy and calorimetry”, Biofilms 7th Annual workshop, Malmö, 24-25 Oct, 2011

• Laura Adamonytė, Justas Barauskas, Daiva Tauraitė, “Behavior of Amide Derivatives of Monooleoyl and Monolinoleoyl Glycerol Synthesis and Aggregation”, Biofilms 7th Annual workshop, Malmö, 24-25 Oct, 2011

• M Dorkhan, L Chavez de Paz, G Svensäter and JR Davies, ”Effect of surface roughness on adhesion of Streptococcus oralis to titanium surfaces”, Biofilms 7th Annual workshop, Malmö, 24-25 Oct, 2011

• Magnus Falk, Viktor Andoralov, Zoltan Blum, Javier Sotres, Dmitry Suyatin, Tautgirdas Ruzgas, Thomas Arnebrant, and Sergey Shleev, “”, Biofilms 7th Annual workshop, Malmö, 24-25 Oct, 2011

• Marija Jankunec, Justas Barauskas, Markus Johnsson and Vitaly Kocherbitov, “The aqueous phase behavior of dioleoylphosphatidylcholine”, Biofilms 7th Annual workshop, Malmö, 24-25 Oct, 2011

NATIONAL

LOCAL


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• V. Krikstolaityte, A. Barrantes, J.Sotres, T. Arnebrant, A. Ramanavicius1, T. Ruzgas, “Physicochemical properties of gold nanoparticle multilayers”, Biofilms 7th Annual workshop, Malmö, October 24-25, 2011

• Znamenskaya Y, Engblom J, Arnebrant T, Kocherbitov V, “Effect of Hydration on Thermodynamic Properties of Pig Gastric Mucin”, Biofilms 7th Annual workshop, Malmö, 24-25 Oct, 2011

BSc AND MSc EDUCATION

BSc-LEVEL

MSc-LEVEL

• Biomedical laboratory science (HS)

• Biomedical Technology TELMah (TS & HS)

• Dentistry program (OD)

• Biomedical Methods and Technology (HS)

• Materials science (TS & HS)

• Dentistry program (OD)

OTHER ACTIVITIES BY CENTER MEMBERSAnn Wennerberg has been opponent for PhD theses at the University of Ghent, Belgium, and Gunilla Nordin Fredrikson has been opponent at a half-time control at the Department of Laboratory Medicine, Lund University. Tautgirdas Ruzgas, Gunilla Nordin Fredrikson, Håkan Eriksson, Anette Gjörloff Wingren, Johan Engblom, Thomas Arnebrant. Ann Wenner-berg, Ryo Jimbo, Liselott Lindh, Claes Wickström, Julia Davies, Gunnel Sven-säter, and Per Ståhle were members of examination committees for PhD

defences on 21 occasions during 2011. Ann Wennerberg has been an external reviewer for a promotion to professor at Umeå University. Gunnel Svensäter has been an expert reviewer for an application for promotion at the Karo-linska Institute.

Center members have been referees for international journals on a regu-lar basis. The list of journals includes; Acta Biomaterialia, Acta Odonto-logica Scandinavica, Atherosclerosis, Archives of Oral Biology, Biofouling,

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Biomaterials, Biomacromolecules, Bioelectrochemistry, Cancer Letters, Caries Research, Cellular Immunol-ogy, Chemistry and Physics of Lipids, Circulation, Clinical Chemistry, Clini-cal Oral Implants Research, Clini-cal Implant Dentistry and Related Research, Dental Materials Journal, Electroanalysis, Electrochemistry Communications, European Journal of Dental Education, European Jour-nal of Oral Sciences, Experimental Lung Research, Human Immunology, Experimental Biological Medicine, International Journal of Dentistry, International Journal of Fracture, International J. Nanomedicine, Inter-national Journal of Oral and Maxillo-facial Research, International Journal of Prosthodontics, International Jour-nal of Solids and Structures, Journal of Anatomy, Journal of Applied Mechan-ics, Journal of Inorganic Biochemistry, Journal of Artificial Organs, Journal of Biomedical Materials Research, Jour-nal of Biomedical Materials Research: Part B, Journal of Colloid and Interface Science, Journal of Dental Research, J Mechanical Engineering, Journal of Medical Microbiology, Journal of Oral Rehabilitation, Journal of Peri-odontal Research, Journal of Pros-thodontic Research, Journal of Royal Society Interface, Journal of Periodon-tal Research, Langmuir, Oral Oncol-ogy, PLoS ONE, Physical Review B, Process Biochemistry, Protein and Peptide Letters, Proteome Science, Stroke, Vitae, Vaccine, Thrombosis and Haemostasis.

Tautgirdas Ruzgas is on the editorial board for Nonlinear Analysis: Model-ling and Control, and Per Ståhle is on the editorial board for the Internation-al Journal of Fracture. Ann Wenner-berg is on the editorial board for Acta

Biomaterialia, International Journal of Prosthodontics, and Acta Bioma-terialia. Ryo Jimbo is on the editorial board for Clinical Implant Dentistry & Related Research, and Journal of Oral and Maxillofacial Research.

Ann Wennerberg was co-ordinator, and Gunnel Svensäter was the local co-ordinator at Malmö University, of the National Graduate Research School in Odontology. Gunilla Nordin Fredrikson was examiner of papers for the degree of Bachelor of Science at Health and Society, Malmö University. Thomas Arnebrant was examiner of the master’ s theses in the programme “Biomedical Methods and Technol-ogy”, Health and Society, Malmö University, and on the Faculty Board, the Board for Research and Research Education at the Faculty of Health and Society, Malmö University. Anette Gjörloff Wingren was chair of the Education Board at the Faculty of Health and Society (Malmö Univer-sity) as well as chair of the Board for Constitution of New Programmes (Malmö University). Gunnel Svensäter was chair of the Malmö University Associate Professor Board as well as a member of the Research Commit-tee, Faculty of Odontology, Malmö University. Julia Davies was Director of Postgraduate Studies as well as co-ordi-nator for Basic Biology in Undergrad-uate Curriculum and a member of the Research Committee, Faculty of Odontology, Malmö University. Taut-girdas Ruzgas was on the evaluation board for the appointment of associate professors at Malmö University. Johan Engblom was a member of the Faculty Board at the School of Technology, Malmö University, and the Education Board (UN) at the Faculty of Health and Society, Malmö University. Claes


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Wickström was Chairman of the Curriculum Committee and a member of the Education Committee, Faculty of Odontology, Malmö University. Claes Wickström and Jessica Neilands were members of the Staff Development and Education Group, Malmö University.

Christina Bjerkén and Gunilla Nordin Fredrikson were reviewers of grants for the Swedish Research Council during 2011. Gunilla Nordin Fredrikson has also been a reviewer on the Regional Ethical Review Board in Lund since 2010. Ann Wennerberg was a reviewer of health research awards 2011for The Irish Health Research Board and of centres of excellence for the Research Council of Norway. Vitaly Kocherbi-tov was project application reviewer for the Danish Council for Independ-ent Research. Anna Wennerberg was a

member of the Abstract Committee for the 20th Annual Scientific Congress of the European Academy for Osseointe-gration. Per Ståhle is a member of the International Union of Theoretical and Applied Mechanics.

Gunnel Svensäter has been a member of the Scientific Advisory Board, Swed-ish Council on Technology Assess-ment in Health Care (SBU). Gunilla Nordin Fredrikson was consulted as a judgement expert for biomedical scientist programmes in Sweden by the National Agency for Higher Education and was also convener of the Evalua-tion Committee of DPLU (Diabetes Programme Lund University). She was also a reviewer for PhD student and mobility grant applications within the programme.


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CONTACT INFORMATIONBiofilms – Research Center for BiointerfacesFaculty of Health and SocietyMalmö UniversitySE-205 06 Malmö, Sweden

Center Director: Dr. Anna HolmbergTel: +46-(0)40-665 79 35 (Anna Holmberg)+46-(0)40-665 75 52 (Administrative Co-ordinator Eva Nilsson)

E-mail: [email protected]/biofilms

Visiting address:Skåne University Hospital, SUS (Entrance 49)Malmö

Progress Report 2011 Biofilms

Documents

Transcript of Progress Report 2011 Biofilms