Production of clean seed
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description
Y Y Y Y Y. Y Oe Y Oe Y Oe Y Oe Y Oe. Y Oe Y Oe Y Oe Y Oe Y Oe. Y O Y O Y O Y O Y O. Production of clean seed. The virus-free program at WRPIS (Pullman, WA). - PowerPoint PPT Presentation
Transcript of Production of clean seed
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Production of clean seed
The virus-free program
at WRPIS (Pullman, WA)
1) History
2) The “TEST”
3) The “clean-up”
4) The future
History
• 1983 >70% infected w/ BCMV
• 1985 original protocol
• 1994 leaf tissue testing
• 23.3% of collection virus-free
The “TEST”
• Tissue harvest
• ELISA testing
• Clean
• “Dirty”
The “clean up”
• <10% BCMV infection
– rouging
• >10% BCMV infection
– single planting, harvest & testing
• Re-combining clean seed
¿ The future ?
• Widely recognized protocol
• Adapted by other germplasm collections
• Virus testing technician
• Part-time help? Soft money?
• Suggestions?????
