Product Guide 2013 - Bioline€¦ · Nucleic Acid Isolation 14 DNA Kits, RNA Kits, Column-free...

Product Guide 2013Product Guide 2013

The PCR Company

Real-Time PCR Mixes

PCR Enzymes & Mixes

RNA Analysis

Cloning and Protein Expression

Nucleic Acid Isolation

Nucleotides

Molecular Weight Markers

Essential Reagents

Bioline, The PCR Company, is changing. Following our acquisition by Meridian Biosciences Inc., a world-leading

manufacturer and supplier of antibodies, viral antigens and critical assay reagents, we have undergone a number

of exciting developments. Through new investment, we have made significant enhancements in both our R&D

and manufacturing capabilities, designed to develop new innovative products and further enhance the quality,

performance and reliability of our leading products.

Featured in this guide, are results from this investment:

• The SensiFAST™ range is a comprehensive set of highly-optimised products designed to deliver outstanding

performance and reproducibility for real-time experiments with DNA and RNA templates on all standard and

fast real-time PCR instruments.

• MyTaq™, MyFi™ and RANGER, based on the latest technology in PCR enzyme preparation, represent a new

generation of very high-performance PCR products engineered to increase affinity for DNA. This feature results

in significant improvements to yield, sensitivity, and speed giving access to high quality results faster than ever.

• There are also significant improvements to our ISOLATE DNA and RNA purification products. In the majority of

molecular biology experiments, the key to obtaining great results is the quality and purity of the starting nucleic

acid, ISOLATE II kits are designed to be easy-to-use and will outperform kits from the current market leaders.

With R&D labs In the UK, Germany and Australia and manufacturing at the same locations, continued investment

allows us to better support our customers, both existing and new. Bioline is committed to invest in innovative

technologies aimed at developing unique products for PCR and real-time PCR to bring you the reagents you

need to drive your research forward.

Bioline is one of the world’s few manufacturers of the highest purity dNTPs within a portfolio of more than 200

reagents and kits for PCR and molecular biology. It doesn’t stop there, we have ISO 9001:2008 certification and

we are continually looking at ways of improving our processes to bring you the best value reagents with class

leading performance, reproducibility and reliability.

Bioline products and services are available globally through subsidiary companies and our growing list of

distribution partners in more than 40 countries in the USA, Europe, the Middle East, Africa, Latin America and

the Pacific Rim.

At Bioline, we have been serving the life science community for over 20 years and we are looking forward to

even greater successes as we begin a new chapter in our history.

Welcome to your new 2013 Product Guide

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www.bioline.com | The PCR Companywww.bioline.com | The PCR Company

Contents at a GlancePCR Enzymes & Mixes 6

Hot-Start DNA Polymerases, High Fidelity

DNA Polymerases, Polymerases for Routine

Applications, Polymerases for Specialised

Applications

Real-Time PCR Mixes 10

Reagents with SYBR, Reagents for Probe,

Reagents for Other Techniques, Reagents for

SYBR One-Step, Reagents for Probe One-Step

Nucleic Acid Isolation 14

DNA Kits, RNA Kits, Column-free Isolation

Nucleotides 18

dNTP Sets and Mixes, NTPs

Molecular Weight Markers 20

DNA Markers, Crystal Loading Buffers,

HyperPAGE Prestained Protein Markers

Cloning 22

Cloning Cells, Protein Expression, Cloning

Reagents, Antibiotic Solutions

RNA Analysis 24

cDNA synthesis, RNA reagents

Essential Reagents 25

Agaroses, Reagents, Crystal Electrophoresis

Running Buffers, Crystal Loading Buffers, Crystal

General Buffers, PCR Buffers, PCR Additives and

Water, Antibiotic Solutions, Genomic DNA

Index

Ordering information 33

Terms and Conditions of Sale 35

Trademarks 35

3

Contents

For more information please visit www.bioline.com

Confidence and trustFor 20 years Bioline has been providing custom-made solutions to industry and

commercial partners, both large and small, for their specific product needs and

we welcome new partners who wish to take advantage of our expertise and

technologies. When the requirements for optimizing the performance of your

application are beyond the scope of our standard product range, we invite you

to use our custom-made, bulk and OEM services.

Each custom, bulk or OEM arrangement features a comprehensive service

package and includes:

• Custom product configurations

• Custom bulk packaging requirements

• Private label OEM manufacturing

• Validation support information

• Scheduled delivery to fit your needs

• Fast-track technical and scientific support

Quality is at the heart of what we do, our state-of-the-art manufacturing

sites in the UK, Germany and Australia are ISO 9001:2008 certified and we

continuously improve the excellence of our operational and quality assurance

systems. All stages of the manufacturing process are controlled and monitored

using stringent quality control procedures to guarantee the highest quality and

lot-to-lot reproducibility.

Our competence in molecular biology reagents manufacturing includes

extensive experience in bioreactors and fermentation, chemical synthesis,

nucleotide chemistry, PCR enzymes chemistry, molecular biology methods,

DNA and RNA purification, competent cell preparation, downstream processing

on any scale, as well as product fill and finish.

Excellence in PCR and bioreagents manufacturing

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www.bioline.com | The PCR Company

For the last 20 years, Bioline has been engaged in the development and manufacturing of one of the broadest

portfolios of premium quality PCR enzymes, for a wide range of applications. Each DNA polymerase is

different and conditions to achieve optimal results need varying characteristics. For your convenience and to

achieve optimal PCR, many of our most popular PCR enzymes are also available in practical, ready-to-use 2x

mastermixes which contain polymerase, dNTPs, MgCl2 and additional additives.

Standard DNA Polymerases

MyTaq™ is a new generation of very high

performance PCR polymerases, designed to deliver

outstanding results on all templates. MyTaq is

engineered to increase affinity for DNA, resulting

in significant improvements to yield, sensitivity and

speed. MyTaq is the perfect choice for all PCR

assays.

MangoTaq™ contains a colored reaction buffer to

make it an ideal choice for standard high-throughput

applications, as it allows the user to load directly on a

gel, without the need for separate gel-loading buffer.

BIOTAQ™ is a highly purified thermostable DNA

polymerase offering very high yield over a wide range

of PCR templates, and is suitable for routine assays.

DNA Polymerases for Specialized Applications

RANGER is a novel, antibody-mediated, hot-start

enzyme blend that offers 3.5x higher fidelity than

native Taq, designed to amplify templates from 10kb

or greater with extreme sensitivity.

BIO-X-ACT™ Short is specifically designed for

difficult/problematic PCR applications requiring both

high processivity and fidelity that would normally fail

with Taq DNA polymerase.

High-fidelity DNA Polymerases

ACCUZYME™ is a thermostable enzyme possessing

proofreading activities, offering high-fidelity, even

with demanding applications. ACCUZYME produces

blunt-ended amplicons up to 5kb.

VELOCITY is an ultra-fast thermostable enzyme

possessing proofreading exonuclease activity.

VELOCITY has high processivity, resulting in shorter

extension times, higher yield and the ability to amplify

long templates in a fraction of the time, even from low

template concentrations.

Hot-Start DNA Polymerases

MyTaq™ HS is a new generation of very high

performance, antibody-mediated hot-start PCR

products, designed to give outstanding results with

complex genomic DNA templates. MyTaq HS is

designed to increase affinity for DNA, so resulting

in significant improvements in yield, sensitivity and

speed.

IMMOLASE is a chemically-modified hot-start

containing a heat labile blocking group and requires

incubation at 95°C for 10 minutes, giving improved

specificity as compared to standard polymerases.

MyFi™ is a novel, antibody-mediated, hot-start

enzyme blend that offers higher fidelity and enhanced

specificity than native Taq. MyFi is ideally suited for

PCR amplification of difficult targets which require

both high processivity and higher fidelity.

Premium quality PCR enzymes

5

Prem

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CR

enzymes

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For more information please visit www.bioline.com/polymerases

• Rapid extraction protocol – High yield PCR-ready DNA in 15 minutes

• Replaces complicated DNA extraction procedures

• Perfect for high-throughput genotyping from mammalian tissues

• Convenient single-tube reaction minimizes contamination

• MyTaq™ HS Mix for fast and highly-specific amplification

MyTaq Extract-PCR Kit offers a quick and easy alternative for the extraction and amplification of DNA

from a variety of tissue types. Many other DNA extraction methods can be laborious and time consuming,

using phenol extraction, overnight incubations often combined with column purification. These methods

all contribute to the total time taken to prepare PCR-ready DNA and are rarely suited to medium or high-

throughput experiments. In addition, the longer protocols often require large amounts of starting material and

need to be further optimized for efficient extraction of DNA, particularly when samples are solid tissues such

as mouse tail or mouse ear.

When used with the same starting material, the MyTaq Extract-PCR Kit gave a better yield and was more

sensitive, compared to other suppliers of similar kits. Bioline’s MyTaq Extract-PCR Kit offers a convenient

alternative for the extraction of DNA for applications such as mouse genotyping and sequencing (Fig. 1).

MyTaq™ Extract-PCR kitA quantum leap for PCR

Fig. 1. MyTaq Extract-PCR was used to extract and amplify genomic DNA from 3mg pieces of mouse tail. Genomic DNA was extracted in a 100µl reaction and 1µl of the supernatant used for the PCR reactions. After an initial 1 in 30 dilution, serial two fold dilutions of the supernatant were used with MyTaq HS Mix for amplification of a 1kb fragment from mouse b-actin (Lanes 1 – 11). Marker - EasyLadder I (M).

New Product Highlights

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1 2 3 4 5 6 7 8 9 10 11 1 2 3 4 5 6 7 8 9 10 11

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• Unique blend of RT and MyTaq HS for highest specificity

• Ultra-sensitive detection from low-copy genes

• Overcomes secondary structure in difficult and GC-rich targets

• High-quality, full-length cDNA from as little as 3pg of total RNA

• Convenient to use all-in-one mix

MyTaq One-Step RT-PCR has been formulated for highly reproducible first-strand cDNA synthesis and

subsequent PCR in a single tube. The combination of the latest advances in buffer chemistry together with

a reverse transcriptase and hot-start DNA polymerase system ensures that MyTaq One-Step RT-PCR Kit

produces fast, highly-specific and ultra-sensitive one-step RT-PCR, from either total RNA or mRNA using

gene-specific primers. The reverse transcriptase is thermally stable, so for RNA transcripts that form stable

secondary structures at low temperatures (including GC-rich sequences), higher temperatures can be used

(fig. 1), ensures high cDNA yields from all RNA.

MyTaq One-Step RT-PCR specially formulated enzyme blend ensures highly efficient and sensitive

transcription from as little as 3pg total RNA and is robust enough to work under different cycling conditions

(fig. 2). Even greater sensitivity and specificity can be achieved by use of gene-specific primers, maximizing

amplification of these targets while minimizing amplification of other sequences.

MyTaq One-Step RT-PCR Kit reduces reaction-to-reaction variability and helps prevent contamination

by minimizing hands-on steps. It is ideal for determining the presence or absence of RNA templates and

quantifying expression through qualitative, semi-quantitative or quantitative analysis of RNA transcription levels.

The one-step format is also perfect for the synthesis of double-stranded cDNA products for subsequent gene-

expression analysis.

MyTaq™ One-Step RT-PCR KitAll together better for RNA analysis

Fig. 2. Sensitivity of MyTaq One-Step RT-PCR Kit A serial dilution of mouse total RNA (30ng, 3ng, 300pg, 30pg and 3pg respectively) in duplicate was used with RN18S-1000 primers (to produce a 1kb fragment), that was analysed on a 1% agarose gel (BIO-41025). HyperLadder 50bp (M) (BIO-33039).

M 0pg 3pg 30pg 300pg 3ng 30ng

Fig. 1. Broad temperature range of MyTaq One-Step RT-PCR Kit A serial dilution of mouse total RNA in duplicate (10ng, 2ng, 400pg, 80pg, 16pg and 3pg; lanes 1-6 respectively), was used in a reverse transcription reaction for 30min and then amplified with RN18S-1000 primers to produce a 1kb fragment. HyperLadder II (M) (BIO-33039). The reverse transcriptase in the MyTaq One-Step RT-PCR Kit was able to deliver high quality cDNA even at 50°C over a broad dynamic range.

1 2 3 4 5 6 M

50°C/30min

45°C/30min

42°C/30min


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With over 20 years of experience, Bioline now produces one of the broadest portfolios of premium quality

PCR Enzymes. This includes the new MyTaq DNA Polymerase product range, a new generation of very high

performance PCR products, designed for significant improvements to yield, sensitivity and speed.

* Includes dNTPs and MgCl2 in the polymerase buffer

Pro

per

ties

Tem

pla

te L

engt

h

Hot

-Sta

rt

Pro

ofre

adin

g

Hig

h P

roce

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ity

Ava

ilab

le M

ixes

Mix

es

Ap

plic

atio

ns

Long

Ran

ge P

CR

(ove

r 10

kb)

Hig

h S

pec

ifici

ty A

ssay

s

Blu

nt E

nd C

loni

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TA C

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GC

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late

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Hig

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de

Sam

ple

PC

R

Fast

PC

R

Dire

ct G

el L

oad

ing

Col

ony

PC

R

MyTaq™ HS * Up to 5kb

IMMOLASE™ Up to 5kb

MyFi™ * Up to 10kb

VELOCITY Up to 10kb

ACCUZYME™ Up to 5kb

RANGER * Up to 25kb

BIO-X-ACT™ Short Up to 8kb

MyTaq™ * Up to 5kb

MangoTaq™ Up to 5kb

BIOTAQ™ Up to 5kb

PCR Enzyme Selection Table

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Each DNA Polymerase has different characteristics and for optimal results, it is crucial to choose the enzyme

that suits your individual application. For your convenience and to achieve optimal PCR, many of our most

popular PCR enzymes are also available in practical, ready-to-use 2x mastermixes which contain polymerase,

dNTPs, MgCl2 and additional proprietary additives.

Pro

per

ties

Tem

pla

te L

engt

h

Hot

-Sta

rt

Pro

ofre

adin

g

Hig

h P

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ity

Ava

ilab

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ixes

Mix

es

Ap

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Long

Ran

ge P

CR

(ove

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kb)

Hig

h S

pec

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ty A

ssay

s

Blu

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nd C

loni

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TA C

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GC

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late

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Sam

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PC

R

Fast

PC

R

Dire

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oad

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Col

ony

PC

R

MyTaq™ HS * Up to 5kb

IMMOLASE™ Up to 5kb

MyFi™ * Up to 10kb

VELOCITY Up to 10kb

ACCUZYME™ Up to 5kb

RANGER * Up to 25kb

BIO-X-ACT™ Short Up to 8kb

MyTaq™ * Up to 5kb

MangoTaq™ Up to 5kb

BIOTAQ™ Up to 5kb

Suitable Recommended

9

PC

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nzyme S

election Guid

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Real-time PCR is one of the most powerful and sensitive gene analysis techniques available and has become

the standard in most laboratories. Its effectiveness, particularly at amplification and quantification of low levels of

nucleic acids, has driven the emergence of numerous applications, including cellular mRNA and quantification,

biomarker discovery and validation, microbial quantification, and detection of extremely low copy targets

for forensic investigations. The increase in the use of real-time PCR has increased the demand for a higher

throughput and faster assays, to reduce the overall protocol time. This has been achieved by improvements in

instruments, using faster enzymes (without sacrificing accuracy), and shortening or even combining PCR steps.

The new SensiFAST Kits have been designed to take up this challenge, with an antibody mediated hot-start and

very rapid enzyme-extension rates this enables fast cycling conditions, the SensiFAST formulations provide the

fastest cycling times whilst maintaining the performance, reliability and high reproducibility of conventional real-

time PCR reagents.

Selection of detection chemistryThe two types of chemistries that have been developed for real-time PCR are:

• Intercalating dye chemistry (including SYBR® Green I dye)

• Probe detection chemistry (including TaqMan®, Scorpions® and molecular beacon probes)

SYBR Green I dye chemistrySYBR Green I dye fluoresces when bound to double-stranded DNA, so the amount of fluorescence is directly

proportional the amount of double-stranded DNA. So if fluorescence is measured after each successive round

of amplification in a PCR, the increase in PCR product can be determined.

Probe detection chemistryAn oligonucleotide probe is constructed with a fluorescent reporter dye bound to the 5’ end and a quencher

on the 3’ end. The proximity of the quencher to the reporter dye greatly reduces the fluorescence emitted. If

the target sequence is present, the probe anneals and is cleaved by the 5’ nuclease activity of the Taq DNA

polymerase during extension, increasing the fluorescence emitted. Additional reporter dye molecules are cleaved

with each cycle, resulting in an increase in fluorescence intensity proportional to the amount of PCR product.

Advantages Disadvantages

SensiFAST SYBR

• Does not require expensive probes, so costs are reduced

• Multiple dye molecules can bind to a single amplified target sequence, increasing sensitivity

• The ability to perform melt curve analysis provides a quality control step to verify amplification of the desired amplicon

• Less specific than probes – only primers determine specificity

• Non-specific binding of SYBR dye to any double-stranded DNA may generate false positives

• Not possible to multiplex multiple gene targets

SensiFAST Probe

• Specific hybridization between probe and target, significantly reduces background and false positives

• Multiple targets can be detected in the same reaction, reducing cost and improving precision

• It is possible to distinguish between similar sequences with small differences e.g. SNPs

• A unique probe has to be synthesized for each target sequence

• Cannot perform quality control step melt curve analysis

SensiFAST™- Real-Time PCR Solutions

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• Superior allele calling – clear discrimination leading to fewer failures

• Tight fluorescence clusters – perfect for difficult SNP loci or very low template amounts

• Faster run time – enabling more experiments per day

• Designed for dual-labeled probe assays – including TaqManTM probe-based assays

The new Bioline SensiFAST Genotyping Kit has been developed for highly reproducible genotyping of

sequence variants, including difficult single nucleotide polymorphisms (SNPs) loci and has been validated on

commonly used real-time instruments. The SensiFAST Genotyping Kit contains a combination of the latest

advances in buffer chemistry and enhancers, together with an antibody-mediated hot-start DNA polymerase

system. This ensures that the SensiFAST Genotyping Kit produces highly-specific and ultra-sensitive real-time

PCR with clear allelic discrimination, providing tight allele clustering and outstanding separation to ensure high

call rates, enabling reproducible and accurate results.

SensiFAST™ Genotyping KitExceed the limit

DNA samples from three Caucasian patients were genotyped using a custom TaqMan™ Drug Metabolism Genotyping Assay with the SensiFAST Genotyping Kit, using a set of thirty replicates for each sample. Cycling conditions: 95oC 3min, 35 cycles 95oC 10s, 60oC 30s. The results illustrate the SensiFAST Genotyping Kit gives very tight clusters between the replicates.

A

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Endpoint Fluorescence Scatter Plot


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Bioline has a comprehensive range of highly optimised products designed to deliver outstanding results for real-

time experiments on both DNA and RNA templates.

SensiFAST has been specially developed to deliver optimal real-time PCR results in a fraction of the time using

standard as well as the new faster real-time PCR instruments.

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No-ROX Kit

SensiFAST™ SYBR Hi-ROX Kit

SensiFAST™ SYBR Lo-ROX Kit

SensiFAST™ SYBR & Fluorescein Kit

Pro

be

Ass

ays

SensiFAST™ Probe No-ROX Kit*

SensiFAST™ Probe Hi-ROX Kit

SensiFAST™ Probe Lo-ROX Kit

SensiFAST™ Genotyping Kit

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SensiFAST™ HRM Kit

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No-ROX One-Step Kit

SensiFAST™ SYBR Hi-ROX One-Step Kit

SensiFAST™ SYBR Lo-ROX One-Step Kit

SensiFAST™ SYBR & Fluorescein One-Step Kit

Pro

be

Ass

ays SensiFAST™ Probe

No-ROX One-Step Kit*

SensiFAST™ Probe Hi-ROX One-Step Kit

SensiFAST™ Probe Lo-ROX One-Step Kit

SensiFAST™ Real-Time PCR Selection Table

12

Sen

siFA

ST

Rea

l-Tim

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CR

Sel

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The Selection Table below will enable you to choose the most appropriate reagent for your real-time instrument

and application.

For SensiMix™ Selection Table see www.bioline.com/realtime

* Used for all instruments when multiplexing Recommended

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No-ROX Kit

SensiFAST™ SYBR Hi-ROX Kit

SensiFAST™ SYBR Lo-ROX Kit

SensiFAST™ SYBR & Fluorescein Kit

Pro

be

Ass

ays

SensiFAST™ Probe No-ROX Kit*

SensiFAST™ Probe Hi-ROX Kit

SensiFAST™ Probe Lo-ROX Kit

SensiFAST™ Genotyping Kit

HR

M

SensiFAST™ HRM Kit

RN

A T

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pla

tes

SY

BR

Gre

en

Ass


No-ROX One-Step Kit

SensiFAST™ SYBR Hi-ROX One-Step Kit

SensiFAST™ SYBR Lo-ROX One-Step Kit

SensiFAST™ SYBR & Fluorescein One-Step Kit

Pro

be

Ass

ays SensiFAST™ Probe

No-ROX One-Step Kit*

SensiFAST™ Probe Hi-ROX One-Step Kit

SensiFAST™ Probe Lo-ROX One-Step Kit

13

SensiFA

ST R

eal-Time P

CR

Selection Table

For more information please visit www.bioline.com/realtime

See p

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Bioline ISOLATE II Kits for nucleic acid isolation are perfect to start the analysis of your samples. The kits are designed

for fast and efficient isolation of DNA and RNA from a wide range of biological materials, including animal tissue,

cultured cells, buccal swabs, blood, bacterial cells, plant tissue as well as PCR products and agarose gels.

Bioline, the PCR company, has a well established and proven track record of providing superior quality molecular

biology reagents. The ISOLATE II range of nucleic acid isolation kits are designed to make it easier for our customers

to obtain high quality, reliable reagents for every step of their research from a single source. The kits perfectly

complement our well known range of downstream PCR reagents such as DNA polymerases, polymerase mixes, real-

time PCR kits, reverse transcriptase and cloning products.

Featured Kit: ISOLATE II RNA Mini KitFEATURES

• Rapid protocol: 30 min/6 preps

• High-purity RNA (A260/A280 ratio 1.9 - 2.1)

• RNA integrity number (RIN) >9

• RNA binding capacity up to 200µg

• Filters (shredders) included to enhance sample homogenization

• DNase I included for complete removal of genomic DNA

• Isolated RNA is ready for downstream applications

Fig. 1 High-quality RNA RNA was isolated from HeLa cells using ISOLATE II RNA Mini Kit and analyzed by the Bioanalyzer 2100 (Agilent Technologies). A) The quality of RNA was found to be exceptional (RIN: 9.2) and B) highly reproducible

B)

A)

New Nucleic Acid Isolation Kits

14

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• Outstanding purity and yields

• Rapid and easy to use

• Designed for applications requiring extreme sensitivity and reproducibility

• 10 kits optimized to cover a wide range of starting materials

• Over 40 detailed support protocols

The new range of Bioline ISOLATE II high performance nucleic acid isolation kits offer industry leading

performance for the most demanding downstream applications including real-time PCR, sequencing, arrays,

restriction analysis, cloning and genotyping.

ISOLATE IIA precious find

ISOLATE II DNA KitsSureClean

Proteinase K

Nu

cleic

Aci

d

Puri

�cat

ion

Ap

plic

atio

ns

Ap

plic

atio

ns

ISOLATE II RNA KitsTRIsure

BLOODWhole blood, serum

Plasma and buffy coatBacterial/viral DNA in blood

PCR AND GEL PCR products

DNA from agarose gels

EUKARYOTIC CELLSCultured mammalian cells

Cell linesYeast

BACTERIAL CELLSGram positiveGram negative

CLINICAL AND FORENSIC SAMPLESParaf�n embedded tissue

Buccal swabs, cryosections Biological �uids

PLANT TISSUEFresh/frozen/driedDung/fecal material

Compost

HUMAN AND ANIMAL TISSUETissue

Rodent tails

CLONINGCompetent cells*

Ligases*

Antibiotics*

Cloning reagents*

OTHERSequencingArrays Restriction AnalysisNorthern/Southern Blotting Electrophoresis*

* Products available at Bioline

END-POINT PCRDNA Polymerases* Hot-Start Polymerases* High-Fidelity Polymerases*

REAL-TIME PCRTwo-Step SYBR Kits*

Two-Step Probe Kits* HRM Kits*

REVERSE TRANSCRIPTION / CDNA SYNTHESISMyTaq One-Step RT-PCR Kit*

Tetro cDNA Synthesis Kit*

Tetro Reverse Transcriptase* RNA reagents*

REAL-TIME PCROne-Step SYBR Kits*

One-Step Probe Kits*

DNA

RNA

15

New

Product H

ighlights

For more information please visit www.bioline.com/isolate

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ation

Bioline ISOLATE II Kits use the latest development in silica-based technology, which selectively binds nucleic

acids allowing separation of contaminants to give highly pure DNA or RNA. For high throughput we offer ultra-

filtration technology in a 96-well format. The isolated nucleic acids are ready-to-use in downstream applications

such as end-point PCR, real-time PCR, cloning, reverse transcription, cDNA synthesis, sequencing, genotyping,

etc. Bioline also provides column-free products, TRIsure and SureClean for RNA and DNA isolation respectively.

Cru

de R

NA

DNA Kits

RNA Kits

Column-free isolation

Yeast

Vir

us

TAE

& T

BE

gels

Soil

Rod

en

t ta

il

Pla

nt

tiss

ue &

cells

P1 c

on

stru

cts

&

cosm

ids

PC

R p

rod

ucts

Para

ffin e

mb

ed

ded

tiss

ue

Inse

ct

Hair

Fu

ngi

Fecal

Cu

ltu

red c

ells

Cry

ose

cti

on

s

Com

post

an

d d

un

g

Cle

an

-up f

rom

re

acti

on m

ixes

Bu

ccal sw

ab

s

Blo

od

/seru

m

Bio

psy

Bio

logic

al fl

uid

s

Bacte

rial cells

An

imal ti

ssu

e

Plasmid Mini Kit

Genomic DNA Kit

PCR & Gel Kit

HT 96 Clean-Up Kit*

Blood DNA Kit

Plant DNA Kit

Fecal DNA Kit

RNA Mini Kit

RNA Micro Kit

RNA Plant Kit

RNA Micro Clean-Up Kit

SureClean (DNA)

TRIsure (RNA)

Nucleic Acid Isolation Selection Table

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Each kit is supplied with a comprehensive Product Manual which contains detailed protocols and additional

helpful information. The kits also include a separate, splash-proof bench-top protocol for quick reference at the

bench.

Cru

de R

NA

DNA Kits

RNA Kits

Column-free isolation

Yeast

Vir

us

TAE

& T

BE

gels

Soil

Rod

en

t ta

il

Pla

nt

tiss

ue &

cells

P1 c

on

stru

cts

&

cosm

ids

PC

R p

rod

ucts

Para

ffin e

mb

ed

ded

tiss

ue

Inse

ct

Hair

Fu

ngi

Fecal

Cu

ltu

red c

ells

Cry

ose

cti

on

s

Com

post

an

d d

un

g

Cle

an

-up f

rom

re

acti

on m

ixes

Bu

ccal sw

ab

s

Blo

od

/seru

m

Bio

psy

Bio

logic

al fl

uid

s

Bacte

rial cells

An

imal ti

ssu

e

Plasmid Mini Kit

Genomic DNA Kit

PCR & Gel Kit

HT 96 Clean-Up Kit*

Blood DNA Kit

Plant DNA Kit

Fecal DNA Kit

RNA Mini Kit

RNA Micro Kit

RNA Plant Kit

RNA Micro Clean-Up Kit

SureClean (DNA)

TRIsure (RNA)

* High Throughput

17

Nucleic A

cid Isolation Selection Table

For more information please visit www.bioline.com/isolate

See p

age 28

-29 for o

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inform

ation

dNTPs Directly from the ManufacturerFor over 20 years, Bioline has been actively involved in the development and manufacture of ultra-pure deoxynucleotides (dNTPs). Our state-of-the-art enzymatic manufacturing processes and facilities, combined with stringent quality assurance, enable us to manufacture dNTPs with the highest level of quality and performance, crucial for sensitive techniques and critical applications in molecular biology.

Bioline is constantly developing and enhancing its production capability and expertize in the nucleotide area. For example, Hydroxymethyl dCTP is a novel nucleotide analogue that can be used to generate PCR products in which cytosine nucleotides are uniformly replaced by hydroxymethylated cytosines, these have exciting applications in forensic DNA analysis and epigenetics.

Performance and SensitivityUltra-pure dNTPs have been validated for use in a variety of molecular biology applications including highly sensitive techniques such as real-time PCR, long range PCR, RT-PCR and low-copy or rare-message assays.

dATP dCTP dGTP dTTP dUTP Hydroxymethyl dCTP

ProductdATP lithium

100mM solutiondCTP lithium

100mM solutiondGTP lithium

100mM solutiondTTP lithium

100mM solutiondUTP lithium

100mM solutionHydroxymethyl dCTP lithium

100mM solution

Nomenclature 2’-deoxyadenosine-5’-triphosphate 2’-deoxycytidine-5’-triphosphate 2’-deoxyguanosine-5’-triphosphate 2’-deoxythymidine-5’-triphosphate 2’-deoxyuridine-5’-triphosphate 5 hydroxymethyl 2’-deoxycytidine-5’-triphosphate

Formula C10H12N5O12P3Li4 C9H12N3O13P3Li4 C10H12N5O13P3Li4 C10H13N2O14P3Li4 C9H12N2O14P3Li4 C11H12N3O14P3Li4

Molecular Weight 514.9g/mol 490.9g/mol 530.9g/mol 505.9g/mol 492.884g/mol 520.9g/mol

lmax pH 7.0 259nm 272nm 252nm 267nm 262nm 275nm

e at lmax @ pH7.0 15.4 E x mmol-1 x cm-1 9.1 E x mmol-1 x cm-1 13.7 E x mmol-1 x cm-1 9.6 E x mmol-1 x cm-1 10.0 E x mmol-1 x cm-1 7.7 E x mmol-1 x cm-1

A250/A260 0.78 ± 0.03 0.82 ± 0.03 1.16 ± 0.05 0.65 ± 0.03 0.75 ± 0.03 0.90 ± 0.03

A280/A260 0.15 ± 0.02 0.98 ± 0.03 0.66 ± 0.03 0.73 ± 0.02 0.38 ± 0.02 1.33 ± 0.03

Concentration 100mM ± 2% 100mM ± 2% 100mM ± 2% 100mM ± 2% 100mM ± 2% 100mM ± 2%

Appearance Clear Colorless Solution Clear Colorless Solution Clear Colorless Solution Clear Colorless Solution Clear Colorless Solution Clear Colorless Solution

pH of Solution 7.5 7.5 7.5 7.5 7.5 7.5

dNTP (HPLC Area) ≥99% ≥99% ≥99% ≥99% ≥99% ≥99%

dNDP (HPLC Area) <1% <1% <1% <1% <1% <1%

DNase, RNase, Nicking Activity

Negative Negative Negative Negative Negative Negative

Storage at -20°C at -20°C at -20°C at -20°C at -20°C at -20°C

Stability ≤24 months ≤24 months ≤24 months ≤24 months ≤24 months ≤24 months

Nucleotides Selection Table

18

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Quality ControlAll Bioline ultra-pure dNTPs undergo stringent quality control to guarantee reliable and outstanding results across manufacturing batches.

Extended Storage LifedNTPs from Bioline have the distinct advantage of being presented as lithium salts, these are more resistant to repeated freeze/thaw cycles helping to keep them free from bacterial contamination during storage (lithium ions being bacteriostatic). Lithium salts are also more soluble than sodium salts in ethanol, so their removal by ethanol precipitation is more efficient, reducing the chances of salt artifacts.

Bulk and Custom OrdersAs a primary manufacturer, Bioline can accommodate requests from micro-liter to multi-liter quantities, we can manufacture special batches with unique formulations, blends and mixes to your requirements.

Visit our dedicated micro site www.bioline.com/custom for more information.

dATP dCTP dGTP dTTP dUTP Hydroxymethyl dCTP

ProductdATP lithium

100mM solutiondCTP lithium

100mM solutiondGTP lithium

100mM solutiondTTP lithium

100mM solutiondUTP lithium

100mM solutionHydroxymethyl dCTP lithium

100mM solution

Nomenclature 2’-deoxyadenosine-5’-triphosphate 2’-deoxycytidine-5’-triphosphate 2’-deoxyguanosine-5’-triphosphate 2’-deoxythymidine-5’-triphosphate 2’-deoxyuridine-5’-triphosphate 5 hydroxymethyl 2’-deoxycytidine-5’-triphosphate

Formula C10H12N5O12P3Li4 C9H12N3O13P3Li4 C10H12N5O13P3Li4 C10H13N2O14P3Li4 C9H12N2O14P3Li4 C11H12N3O14P3Li4

Molecular Weight 514.9g/mol 490.9g/mol 530.9g/mol 505.9g/mol 492.884g/mol 520.9g/mol

lmax pH 7.0 259nm 272nm 252nm 267nm 262nm 275nm

e at lmax @ pH7.0 15.4 E x mmol-1 x cm-1 9.1 E x mmol-1 x cm-1 13.7 E x mmol-1 x cm-1 9.6 E x mmol-1 x cm-1 10.0 E x mmol-1 x cm-1 7.7 E x mmol-1 x cm-1

A250/A260 0.78 ± 0.03 0.82 ± 0.03 1.16 ± 0.05 0.65 ± 0.03 0.75 ± 0.03 0.90 ± 0.03

A280/A260 0.15 ± 0.02 0.98 ± 0.03 0.66 ± 0.03 0.73 ± 0.02 0.38 ± 0.02 1.33 ± 0.03

Concentration 100mM ± 2% 100mM ± 2% 100mM ± 2% 100mM ± 2% 100mM ± 2% 100mM ± 2%

Appearance Clear Colorless Solution Clear Colorless Solution Clear Colorless Solution Clear Colorless Solution Clear Colorless Solution Clear Colorless Solution

pH of Solution 7.5 7.5 7.5 7.5 7.5 7.5

dNTP (HPLC Area) ≥99% ≥99% ≥99% ≥99% ≥99% ≥99%

dNDP (HPLC Area) <1% <1% <1% <1% <1% <1%

DNase, RNase, Nicking Activity

Negative Negative Negative Negative Negative Negative

Storage at -20°C at -20°C at -20°C at -20°C at -20°C at -20°C

Stability ≤24 months ≤24 months ≤24 months ≤24 months ≤24 months ≤24 months

19

Nucleotid

es Selection Table

For more information please visit www.bioline.com/nucleotides

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Bioline offers a wide range of DNA Ladders premixed with Loading Buffer, enabling accurate sizing of DNA ranging between 25bp and 10,000bp and optional mass determination. 5x Sample Loading Buffer is supplied for a ready-to-use format, minimizing the time spent diluting and adding tracking dye to the DNA ladder. Simply transfer the Ladder from the vial to the gel.

The HyperLadder range has been created for accurate sizing of DNA fragments on agarose gels. EasyLadder is designed for shorter runs and can be run on standard or high-throughput agarose gels, the reduced number of bands being easier to distinguish in a short run.

Separation of DNA Ladders

Ladder Separation Range (bp) Leading Dye Color High Intensity Bands (bp)

HyperLadder 1kb 200 - 10,037 Blue 1000 & 10,037

HyperLadder 1kb Plus 200 - 12,007 Blue 1000 & 12,007

HyperLadder 50bp 50 - 2000 Blue 300, 1000 & 2000

HyperLadder 500bp 500 - 5000 Red 1000 & 3500

HyperLadder 100bp 100 - 1013 Blue 300 & 1013

HyperLadder 100bp Plus 100 - 2531 Green 1000

HyperLadder 25bp 25 - 500 Blue 100 & 200

EasyLadder I 100 - 2000 Red Even Bands

EasyLadder II 500 - 5000 Red Even Bands

HyperLadder™ 1kb Plus

1% agarose gel6µl per lane

HyperLadder™ 100bp Plus


3.5% agarose gel5µl per lane

HyperLadder™25bp(formerly HyperLadder™V)


HyperLadder™100bp(formerly HyperLadder™IV)


HyperLadder™500bp(formerly HyperLadder™III)


HyperLadder™50bp(formerly HyperLadder™II)


HyperLadder™1kb(formerly HyperLadder™I)

EasyLadder IIdeal for short runs


EasyLadder IIIdeal for short runs


Molecular Weight DNA Markers

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HyperPAGE is a broad range protein marker prestained with two fluorescent dyes that resolves into 9 bands from 10kDa to 190kDa. HyperPAGE is lyophilized for convenient storage and only needs to be reconstituted prior to use. HyperPAGE II is a broad range protein marker prestained with five fluorescent dyes that resolves into 9 bands 10 to 250kDa. HyperPAGE II is supplied in a convenient ready-to-load format; eliminating the need to heat, reduce, dilute or add loading dyes.

Both are suitable for monitoring the efficiency of electrophoretic migration in SDS-PAGE and the transfer of proteins from gel to membrane in Western blotting, without the need for additional staining dye. Prestained protein markers are suitable for determining the approximate molecular weights of proteins. For accurate sizing, the use of unstained markers is recommended.

190

125

80

50

40

25

20

15

SIZE (kDa)

10

HyperPAGEBand sizes are approximate

HyperPAGE250

140

95

70

55

45

35

25

SIZE (kDa)

10

HyperPAGE IIBand sizes are approximate

HyperPAGE II

HyperPAGE Prestained Protein Markers

21

Hyp

erPAG

E P

restained Protein M

arkers

For more information please visit www.bioline.com/ladders

See p

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Efficient DNA transformation of competent cells is essential for successful Cloning and Protein Expression applications. Bioline offers a wide range of E. coli host strains to meet your requirements.

We maintain rigorous quality control standards to ensure lot-to-lot consistency and the highest transformation efficiencies possible.

Cloning

StrainEfficiency

cfu/μg pUC19

Competency: Chemical C

Electrocomp E

Strain background

Blue white screening (lacZ)

Recombination deficient (recA)

Endonuclease deficient (endA)

Restriction deficient (hsdRK-)

Methyl restriction

deficient (mcrA, mcrB, mrr)

Single-strand ability (F’ episome)

Phage resistance (fhuA, tonA

orT1R)

Unmethylated DNA (dam

dcm)

a-Select Gold Efficiency ≥1 x 109 C K12

a-Select Silver Efficiency ≥1 x 108 C K12

a-Select Bronze Efficiency ≥1 x 107 C K12

a-Select Electrocompetent ≥5 x 109 E K12

a-Select Gold Efficiency T1-Res ≥1 x 109 C K12

a-Select Silver Efficiency T1-Res ≥1 x 108 C K12

CH3-Blue 108 Chemically competent cells ≥1 x 108 C K12


ElectroSHOX Electrocompetent cells ≥1 x 1010 E K12

BIOBlue 108 Chemically competent cells ≥1 x 108 C K12


dam-/dcm- Chemically competent cells ≥1 x 107 C K12

Protein ExpressionStrain Efficiency

cfu/μg pUC19Competency:Chemical C

Electrocomp E

Strain background

Restriction deficient (hsdSB)

Protease deficient (ompT)

T7 RNA polymerase

Deficient in cytosine

metabolism (dcm)

Deficient in galactose

metabolism (gal)

Ideal applications

BL21 ≥1 x 107 C B Non T7 promoter expression

BL21 (DE3) ≥1 x 107 C B T7 promoter expression

BL21 (DE3) PlysS ≥1 x 107 C B Regulation of T7 promoter expression

BL21 (DE3) PlysE ≥1 x 107 C B Regulation of T7 promoter expression

Competent Cells Selection Table

22

Com

pet

ent

Cel

ls S

elec

tion

Tabl

e


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The Bioline Competent Cell Guide is designed to help you select the most appropriate competent cells for your cloning or expression application. Each host strain of E. coli has different characteristics and for optimal results, it is important to use the strain that best suits your application. The Bioline Competent Cell Selection Table below provides a summary of the efficiencies, traits and ideal applications for each Bioline competent cell strain.

Recommended

Cloning

StrainEfficiency

cfu/μg pUC19

Competency: Chemical C

Electrocomp E

Strain background

Blue white screening (lacZ)

Recombination deficient (recA)

Endonuclease deficient (endA)

Restriction deficient (hsdRK-)

Methyl restriction

deficient (mcrA, mcrB, mrr)

Single-strand ability (F’ episome)

Phage resistance (fhuA, tonA

orT1R)

Unmethylated DNA (dam

dcm)

a-Select Gold Efficiency ≥1 x 109 C K12

a-Select Silver Efficiency ≥1 x 108 C K12

a-Select Bronze Efficiency ≥1 x 107 C K12

a-Select Electrocompetent ≥5 x 109 E K12

a-Select Gold Efficiency T1-Res ≥1 x 109 C K12

a-Select Silver Efficiency T1-Res ≥1 x 108 C K12



ElectroSHOX Electrocompetent cells ≥1 x 1010 E K12



dam-/dcm- Chemically competent cells ≥1 x 107 C K12

Protein ExpressionStrain Efficiency

cfu/μg pUC19Competency:Chemical C

Electrocomp E

Strain background

Restriction deficient (hsdSB)

Protease deficient (ompT)

T7 RNA polymerase

Deficient in cytosine

metabolism (dcm)

Deficient in galactose

metabolism (gal)

Ideal applications

BL21 ≥1 x 107 C B Non T7 promoter expression

BL21 (DE3) ≥1 x 107 C B T7 promoter expression

BL21 (DE3) PlysS ≥1 x 107 C B Regulation of T7 promoter expression

BL21 (DE3) PlysE ≥1 x 107 C B Regulation of T7 promoter expression

23

Com

petent C

ells Selection Table

For more information please visit www.bioline.com/cloning

See p

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ation

Bioline RNA Analysis products are manufactured and packaged under the most stringent conditions and are

guaranteed to be RNase/DNase free. The range includes TRIsure and RNA ISOLATE II kits, for column-free and

spin-column isolation of total RNA from cells and tissues, generating pure, intact, high-quality RNA suited to any

downstream application.

Bioline products for first-strand cDNA synthesis include Tetro Reverse Transcriptase, MyTaq One-Step RT-PCR

Kit, Tetro cDNA Synthesis Kit, Oligo (dT)18 and Random Hexamer Primers, and ultra-pure NTPs.

For an RNase free environment, our RiboSafe RNase Inhibitor provides complete inhibition of RNases A, B and

C. Our agaroses, in powder or the convenient ready-to-use pre-weighed tablets are also RNase free.

Cell culture Tissue

DNARNA

cDNA PCR Product

Store at -20°C

Other downstream

processes

Gel Electrophoresis

NTP Mix

TRIsure

ISOLATE II PCR and Gel Kit

ISOLATE II RNA Mini Kit

MyTaq One-Step PCR Kit(with gene-specific primers)

SensiFAST One-Step KitTetro Reverse Transcriptase

RiboSafe RNase InhibitorOligo (dT)18

Random Hexamer Primers

AgaroseAgarose Tablets

EasyLadderHyperLadder

Tetro cDNA Synthesis Kit

CRYSTAL Buffers

SureClean

MyTaq HS MixSensiFAST Kits

RNA Analysis

24

RN

A A

naly

sis

For more information please visit www.bioline.com/rna

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Trusted by scientists all over the world, the Bioline Essentials range provides ultra-pure, high-quality reagents

for molecular biology. This includes core products that deliver outstanding results and compliment our real-time

PCR SensiFAST kits, PCR enzymes, RNA analysis reagents, Markers, Cloning reagents and Competent cells,

offering the convenience of ordering all reagents from one source.

Bioline Essentials includes a comprehensive array of core products for everyday work. This includes Agarose,

available both as powder and convenient pre-weighed tablets and Crystal buffers ready-to-use for loading and

running electrophoresis gels. In addition there is a selection of optimized PCR reaction buffers, additives and

enhancers, antibiotic solutions and other general molecular biology reagents.

• Agaroses, Reagents

• Crystal Electrophoresis

• Running Buffers

• Crystal Loading Buffers

• Crystal General Buffers

• PCR Buffers

• PCR Additives and Water

• Antibiotic Solutions

• Genomic DNA

Essential Reagents

25

Essential R

eagents

For more information please visit www.bioline.com/essentials

See p

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ation

PRODUCT NAME PACK SIZE CAT. NO.

PCR Enzymes & Mixes

Hot-Start DNA Polymerases

MyTaq™ HS DNA Polymerase 250 Units BIO-21111



MyTaq™ HS Red DNA Polymerase 250 Units BIO-21114



MyTaq™ HS Mix 200 Reactions BIO-25045

MyTaq™ HS Mix 1000 Reactions BIO-25046

MyTaq™ HS Red Mix 200 Reactions BIO-25047

MyTaq™ HS Red Mix 1000 Reactions BIO-25048

MyTaq™ Extract-PCR Kit 100 Reactions BIO-21126

MyTaq™ Extract-PCR Kit 500 Reactions BIO-21127

IMMOLASE™ DNA Polymerase 250 Units BIO-21046



MyFi™ DNA Polymerase 250 Units BIO-21117



MyFi™ Mix 100 Reactions BIO-25049

MyFi™ Mix 500 Reactions BIO-25050

High Fidelity DNA Polymerases

VELOCITY DNA Polymerase 250 Units BIO-21098

VELOCITY DNA Polymerase 500 Units BIO-21099

Polymerases for Routine Applications

MyTaq™ DNA Polymerase 500 Units BIO-21105



MyTaq™ Mix 200 Reactions BIO-25041

MyTaq™ Mix 1000 Reactions BIO-25042

MyTaq™ Red DNA Polymerase 500 Units BIO-21108



MyTaq™ Red Mix 200 Reactions BIO-25043

MyTaq™ Red Mix 1000 Reactions BIO-25044

MangoMix™ 250 Reactions BIO-25033

MangoMix™ 1000 Reactions BIO-25034

MangoTaq™ DNA Polymerase 1000 Units BIO-21083



BIOTAQ™ DNA Polymerase 500 Units BIO-21040

BIOTAQ™ DNA Polymerase 2500 Units BIO-21060

BIOTAQ™ PCR Kit 500 Units BIO-21071

Polymerases for Specialised Applications

RANGER DNA Polymerase 250 Units BIO-21121

26

By

Pro

duct

Cat

egor

y | I

nd

ex





RANGER Mix 100 Reactions BIO-25051

RANGER Mix 500 Reactions BIO-25052

BIO-X-ACT™ Short DNA Polymerase 250 Units BIO-21064

BIO-X-ACT™ Short DNA Polymerase 500 Units BIO-21065

BIO-X-ACT™ Short Mix 100 Reactions BIO-25025

BIO-X-ACT™ Short Mix 500 Reactions BIO-25026

Real-Time PCR Mixes

Reagents with SYBR

SensiFAST™ SYBR Hi-ROX Kit 200 Reactions BIO-92002



SensiFAST™ SYBR Lo-ROX Kit 200 Reactions BIO-94002



SensiFAST™ SYBR No-ROX Kit 200 Reactions BIO-98002



SensiFAST™ SYBR & Fluorescein Kit 200 Reactions BIO-96002



Reagents for Probe

SensiFAST™ Probe Hi-ROX Kit 200 Reactions BIO-82002



SensiFAST™ Probe Lo-ROX Kit 200 Reactions BIO-84002



SensiFAST™ Probe No-ROX Kit 200 Reactions BIO-86002



SensiFAST™ Genotyping Kit 200 Reactions BIO-36002



SensiMix™ Capillary Kit 100 Reactions QT405-01



SensiMix™ II Probe Kit 250 Reactions BIO-91002



Reagents for Other Techniques

SensiFAST™ HRM Kit 200 Reactions BIO-32002



27

By P

roduct Category | In

dex



Reagents for SYBR One-Step

SensiFAST™ SYBR Hi-ROX One-Step Kit 100 Reactions BIO-73001

SensiFAST™ SYBR Hi-ROX One-Step Kit 500 Reactions BIO-73005

SensiFAST™ SYBR Lo-ROX One-Step Kit 100 Reactions BIO-74001

SensiFAST™ SYBR Lo-ROX One-Step Kit 500 Reactions BIO-74005

SensiFAST™ SYBR No-ROX One-Step Kit 100 Reactions BIO-72001

SensiFAST™ SYBR No-ROX One-Step Kit 500 Reactions BIO-72005

SensiFAST™ SYBR & Fluorescein One-Step Kit 100 Reactions BIO-75001

SensiFAST™ SYBR & Fluorescein One-Step Kit 500 Reactions BIO-75005

Reagents for Probe One-Step

SensiFAST™ Probe Hi-ROX One-Step Kit 100 Reactions BIO-77001

SensiFAST™ Probe Hi-ROX One-Step Kit 500 Reactions BIO-77005

SensiFAST™ Probe Lo-ROX One-Step Kit 100 Reactions BIO-78001

SensiFAST™ Probe Lo-ROX One-Step Kit 500 Reactions BIO-78005

SensiFAST™ Probe No-ROX One-Step Kit 100 Reactions BIO-76001

SensiFAST™ Probe No-ROX One-Step Kit 500 Reactions BIO-76005

Real-Time PCR Extraction Control

DNA Extraction Control 670 500 Reactions BIO-35028






RNA Extraction Control 560 100 Reactions BIO-38044






Nucleic Acid Isolation

DNA Kits

ISOLATE Fecal Kit 25 preps BIO-52037

ISOLATE Fecal Kit 100 preps BIO-52038

ISOLATE II Plasmid Mini Kit 10 preps BIO-52055



ISOLATE II PCR & Gel Kit 10 preps BIO-52058



ISOLATE II HT 96 Clean Up Kit 10 x 96 preps BIO-52061

ISOLATE II Blood Kit 10 preps BIO-52062



ISOLATE II Genomic Kit 10 preps BIO-52065


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ISOLATE II Plant DNA Kit 10 preps BIO-52068



RNA Kits

ISOLATE II RNA Mini Kit 10 preps BIO-52071



ISOLATE II RNA Micro Kit 10 preps BIO-52074

ISOLATE II RNA Micro Kit 50 preps BIO-52075

ISOLATE II RNA Plant Kit 10 preps BIO-52076

ISOLATE II RNA Plant Kit 50 preps BIO-52077

ISOLATE II RNA Micro Clean-up Kit 10 preps BIO-52080

ISOLATE II RNA Micro Clean-up Kit 50 preps BIO-52081

Column-free Isolation

SureClean Plus 5ml BIO-37047

SureClean Plus 25ml BIO-37048

TRIsure™ 100ml BIO-38032

TRIsure™ 200ml BIO-38033

Nucleotides

dNTP Mix, 100mM Final Conc. 50µmol (500µl) BIO-39028

dNTP Mix, 100mM Final Conc. 200µmol (4 x 500µl) BIO-39029

dNTP Mix, 10mM Final Conc. 10µmol (1ml) BIO-39044

dNTP Mix, 10mM Final Conc. 100µmol (10 x 1ml) BIO-39053

dNTP Mix, 40mM Final Conc. 20µmol (1 x 500µl) BIO-39043

dNTP Set, 100mM 4 x 25µmol (4 x 250µl) BIO-39025

dNTP Set, 100mM 4 x 100µmol (4 x 1ml) BIO-39049

dNTP Set, 100mM 4 x 100µmol (4 x 4 x 250µl) BIO-39026

dNTP Set, 100mM 4 x 500µmol (20 x 4 x 250µl) BIO-39027

dATP, 100mM 25µmol (250µl) BIO-39036

dCTP, 100mM 25µmol (250µl) BIO-39038

dGTP, 100mM 25µmol (250µl) BIO-39037

dTTP, 100mM 25µmol (250µl) BIO-39039

dUTP Mix, 50mM Final Conc. 25µmol (500µl) BIO-39041

dUTP, 100mM 25µmol (250µl) BIO-39035

Hydroxymethyl dCTP, 100mM 25µmol (250µl) BIO-39046

Molecular Weight Markers

DNA Markers

HyperLadder™ 1kb (Formerly Hyperladder™ I) 100 Lanes BIO-33053



HyperLadder™ 1kb Plus 100 Lanes BIO-33068



HyperLadder™ 50bp (Formerly Hyperladder™ II) 100 Lanes BIO-33054

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HyperLadder™ 500bp (Formerly Hyperladder™ III) 100 Lanes BIO-33055



HyperLadder™ 100bp (Formerly Hyperladder™ IV) 100 Lanes BIO-33056



HyperLadder™ 100bp Plus 100 Lanes BIO-33071



HyperLadder™ 25bp (Formerly Hyperladder™ V) 100 Lanes BIO-33057



EasyLadder I 100 Lanes BIO-33062



EasyLadder II 100 Lanes BIO-33063



Protein Electrophoresis

HyperPAGE Protein Marker 10-20 Lanes BIO-33065

HyperPAGE Protein Marker 50-100 Lanes BIO-33066

HyperPAGE II Protein Marker 25-50 Lanes BIO-33067

Cloning and Protein Expression

Cloning Cells

a-Select Competent Cells, Silver Efficiency 2ml (10 x 200µl) BIO-85026

a-Select Competent Cells, Gold Efficiency 1ml (20 x 50µl) BIO-85027

a-Select Bacteriophage T1-Resistant Gold Efficiency

1ml (20 x 50µl) BIO-85030

a-Select Bacteriophage T1-Resistant Silver Efficiency

2ml (10 x 200µl) BIO-85029

a-Select Bronze Efficiency 2ml (10 x 200µl) BIO-85025

a-Select Electrocompetent 1ml (10 x 100µl) BIO-85028

BIOBlue 108 Chemically Competent Cells 1ml (10 x 100µl) BIO-85036

BIOBlue 109 Chemically Competent Cells 1ml (20 x 50µl) BIO-85037

CH3-Blue 108 Chemically Competent Cells 1ml (10 x 100µl) BIO-85039

CH3-Blue 109 Chemically Competent Cells 1ml (20 x 50µl) BIO-85040

dam- dcm- Bacteriophage T1-Resistant 1ml (10 x 100µl) BIO-85044

ElectroSHOX 1ml (10 x 100µl) BIO-85038

Protein Expression

BL21 1ml (10 x 100µl) BIO-85031

BL21 (DE3) 1ml (10 x 100µl) BIO-85032

BL21 (DE3) pLysE 1ml (10 x 100µl) BIO-85034

BL21 (DE3) pLysS 1ml (10 x 100µl) BIO-85033

BL21 ComboPack 1.5ml (15 x 100µl) BIO-85035

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Cloning Reagents

IPTG 5g BIO-37036

IPTG Solution 10ml @ 1M, (240mg/ml) BIO-37082

IPTG Solution 5 x 10ml @ 1M, (240mg/ml) BIO-37083

SOC Medium 10 x 10ml BIO-86033

Antibiotic Solutions

Ampicillin Solution 10ml @ 100mg/ml, in Water BIO-87025

Carbenicillin Solution10ml @ 100mg/ml, in 50%

EthanolBIO-87026

Chloramphenicol Solution 10ml @ 50mg/ml, in Ethanol BIO-87027

Kanamycin Solution 10ml @ 100mg/ml, in Water BIO-87028

Neomycin Solution 10ml @ 50mg/ml, in Water BIO-87029

Tetracycline Solution 10ml @ 12.5mg/ml, in

EthanolBIO-87030

RNA Analysis

cDNA Synthesis

Tetro Reverse Transcriptase 10,000 Units BIO-65050

Tetro Reverse Transcriptase 4 x 10,000 Units BIO-65051

MyTaq™ One-Step RT-PCR Kit 10 Reactions BIO-65047



Tetro cDNA Synthesis Kit 30 Reactions BIO-65042

Tetro cDNA Synthesis Kit 100 Reactions BIO-65043

Oligo (dT)18 27µg BIO-38029

Random Hexamer Primer 25µg BIO-38028

RNA Reagents

RiboSafe RNase Inhibitor 2500 Units BIO-65027

RiboSafe RNase Inhibitor 10,000 Units BIO-65028

Essential Reagents

Agaroses

Agarose Tablets 150g BIO-41028

Agarose Tablets 300g BIO-41027

Agarose, Molecular Grade 100g BIO-41026

Agarose, Molecular Grade 500g BIO-41025

Reagents

Proteinase K 100mg BIO-37037

Proteinase K 1000mg BIO-37039

Proteinase K Solution 5ml @ 20mg/ml BIO-37084

Proteinase K Solution 5 x 5ml @ 20mg/ml BIO-37085

X-GAL 1g BIO-37035

Co-Precipitant, Pink 1.5ml (5mg/ml) BIO-37075

Crystal Electrophoresis Running Buffers

Crystal 10x TBE Buffer 10 Pouches BIO-37104

Crystal 50x TAE Buffer 5 Pouches BIO-37103

Crystal 1x TG Buffer 10 Pouches BIO-37106

Crystal SDS Reagent 50 Tablets BIO-37109

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Crystal General Buffers

Crystal 10x TE Buffer 10 Pouches BIO-37105

Crystal PBS Buffer 100 Tablets (0.5 Litre) BIO-37107

Crystal PBS Buffer 100 Tablets (1 Litre) BIO-37108

PCR Buffers

Hi-Spec Additive, 5x 3 x 1.2ml BIO-37032

MgCl2 Solution, 50mM 3 x 1.2ml BIO-37026

MyTaq™ Reaction Buffer Colorless 4 x 1ml BIO-37111

MyTaq™ Reaction Buffer Red 4 x 1ml BIO-37112

NH4 Buffer, 10x 3 x 1.2ml BIO-37025

PolyMate Additive, 2x 1 x 200µl BIO-37041

DEPC-treated Water 1 Litre BIO-38031

DEPC-treated Water 10 x 10ml BIO-38030

PCR Water (18.2MΩ) 10 x 10ml BIO-37080

Lockable Pipettes, Autoclavable

BioPette™ Plus Four Pack - P3942-SK4

BioPette™ Plus Multichannel (12) Pipettes 5-50µl P4812-50








BioPette™ Plus Single Channel Pipettes 10-100µl P3942-100

BioPette™ Plus Single Channel Pipettes 0.5-10µl P3942-10


BioPette™ Plus Single Channel Pipettes 1,000-10,000µl P3942-10000

BioPette™ Plus Single Channel Pipettes 0.1-2µl P3942-2




FastPette™ Plus Pipette Controller - P2001-W

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Order online at www.bioline.com

Or for general enquiries or to request further information,

please email our customer services at [email protected]

At Bioline we are dedicated to putting our expertise and technology at your service. Our teams of

knowledgable and experienced molecular biology scientists and technical support services are available to

assist you with any scientific or technical issues, product related questions not answered in our online FAQs,

or general troubleshooting. Contact us at [email protected]

Ordering Information

Technical Support

• The latest literature from all fields of life sciences featuring Bioline products

• Tried and tested protocols from the literature to accelerate your research

• Novel applications and approaches from the global scientific community

Bioline has extracted the best in peer-reviewed scientific publications from the global life science community

in order to create an easy to use repository of over 6,000 publications describing the successful use of

Bioline sample and assay technologies. Learn best practice from laboratory scientists around the world who

have published successfully with the Bioline range of industry-leading products – designed to accelerate

breakthroughs in all major fields of life science, from genetics to biochemistry, immunology, microbiology,

neuroscience, cancer research, ecology, plant sciences and veterinary research. The repository has advanced

search features, allowing scientists to find the most interesting and relevant papers in their application area and

covers a complete range of Bioline’s most useful products from SensiFAST to MyTaq.

Thousands of papers cite Bioline products, so if you think we have missed an interesting peer-reviewed

publication, featuring any of our products, please get in touch by email, Twitter, Facebook, or leave a comment

on the blog. With Bioline scholar, not only is it a valuable online resource, but you also gain valuable exposure

and profile for your group or company by letting us share your discoveries with the life science world.

Find out more at www.bioline.com/scholar

Ord

ering Information

For more information please visit www.bioline.com 33

Over the last few years Bioline has developed a comprehensive range

of highly optimized products designed to deliver outstanding results

for end-point and real-time PCR experiments. The SensiFAST™ range

is designed to deliver outstanding results for real-time experiments

with DNA and RNA templates, on all standard and fast real-time PCR

instruments. MyTaq™, MyFi™ and RANGER are a new generation of very

high performance PCR products based on the latest technology in PCR

enzyme preparation, engineered to increase affinity for DNA, resulting in

significant improvements to yield, sensitivity and speed.

Bioline PCR Challenge

Find out more at www.bioline.com/thepcrchallenge

MyFi DNA Polymerase

“MyFi was able to produce high yield clean PCR product when Roche high fidelity plus PCR system failed to produce any product.”The University of Birmingham, Cancer Sciences, Birmingham,

UK

"For verification of difficult to detect genes this product worked great, whereas my usual PCR reagents failed to amplify anything out of my samples."Duke University, Molecular Genetics and Microbiology,

Durham

MyTaq DNA Polymerase

“The speed of MyTaq is surprisingly fast! Only 20s for 1.8k and the amplifying efficiency is also great.”The University of Adelaide/Waite Institute, Plant Research Centre, Adelaide, Australia

"We had barely any signal with the other Taq. When we switch to MyTaq, the bands were there and clean! Nice product!"Institute/Department: UCSF, Surgery, San Francisco

SensiFAST Real-Time PCR

“It is a wonderful product, it makes all the difference between a successful and an unsuccessful experiment.”Alcorn State University, Lorman, Mississippi

“Ct values were much better for the same samples run with ABI 2X SYBR green PCR master mix.”The Ohio State University

We know that once you try these products you will want to keep on using them, so Bioline has issued the

PCR Challenge to any researcher using other polymerases from our competitors. Take the Bioline PCR

Challenge to see what you've been missing. Here is some of the feedback

35

Terms and C

onditions

1. Terminologya. The “Company” shall mean either Bioline Ltd or Bioline GmbH in line with the entity

with whom any order is placed.b. The “Goods” are products supplied by the “Company”. c. The “Buyer” means the person or organisation to whom the Company has

contracted to supply Goods. d. Headings are given for ease of reference only and shall not affect the products

described. e. References to individuals shall be treated as applying to corporations and vice

versa. 2. Contract Conditions These conditions shall apply to every sale made or agreed to be made by

the Company. They shall govern each contract defined as an agreement to sell, either by acknowledgment of the Buyer’s order, or by the dispatch of the Goods requested. These Conditions cannot be varied or waived except with an expressly written approval of the Company. Any conditions submitted by the Buyer, in whatever form, are expressly waived and excluded except where written agreement is given by the Company in exceptional cases.

3. Contract Cancellation The Buyer may not cancel the contract without written agreement of the Company.

If this is given, the Buyer shall indemnify the Company against all losses, damage, claims or action arising as a result of such cancellation.

4. Changes to Contract After accepting an order, the Company may make modifications to the Goods

without informing the Buyer, it being understood that such modifications are improvements, not adversely affecting the performance of the Goods.

5. Prices The price of the Goods does not include the following items: Packaging, delivery, insurance and taxes, duties and other impositions, all of which

shall be borne by the Buyer. The Company shall not modify prices before delivery to the Buyer, except to

reflect additional costs resulting from any alteration or addition to the Buyer’s requirements.

6. Payment Termsa. Payment shall be made in full in the currency invoiced, within 30 days from the date

of the invoice.b. In the event of late payment the Company reserves the right to: Suspend all further deliveries until payment has been made, or cancel the contract

with respect to Goods still to be delivered. In either case this shall not prevent recourse to other rights or remedies available to

the Company.c. The Company may charge interest at 2 per cent per 28 days on any sum overdue,

calculated on a day-to-day basis until final payment. The Buyer shall not be entitled to withhold payment on the grounds of having other claims against the Company.

7. Returns Owing to the temperature requirements of our products, the quality of returned

goods cannot be guaranteed. consequently, we regret that we cannot accept returned goods in the event of a purchasing error.

8. Deliverya. Unless otherwise agreed in writing, all Goods shall be dispatched by methods

judged the most appropriate to ensure the safe delivery of the Goods. Whilst expedient delivery is considered most important, any times quoted for delivery shall be treated as estimates only and the Company shall not be liable for any loss or damage arising from delays in delivery.

b. Delivery is on an ex-factory basis. After delivery, the Goods shall be solely the Buyer’s risk in respect of loss or damage arising from any cause, unless the Buyer notifies the Company in writing: of any damage, discrepancy or shortage in the Goods delivered, within 8 days of delivery or of total loss or non-receipt of the Goods, within 10 days of the Company’s invoice.

In such cases, the Company’s sole obligation shall be limited to the replacement of the Goods in question and the Company shall not be under any other liability whatsoever.

c. Where delivery of any product requires an Export Licence or other authorisation before shipment, the Company shall not be responsible for delivery delays resulting from a delay in the granting, or refusal of, such authorization.

9. Property Title to the Goods shall remain vested in the Company and shall not pass to the

Buyer until the purchase price has been paid in full and received by the Company. However from delivery the Buyer shall keep the Goods fully insured until the time of payment. If the Goods are lost or destroyed before payment, the Buyer shall hold the proceeds of insurance to the order of the Company pending payment. The Buyer agrees not to dispose of or re-sell the Goods before full payment is made for them.

10. Limited Product Warranty The Company warrants that its products will conform to the standards stated in its

product specification sheets in effect at the time of shipment. The Company will

replace free of charge any product that does not conform to the specifications. This warranty limits the Company’s liability only to the replacement of the product.

This warranty is exclusive and the Company makes no other warranty, expressed or implied, including without limitation any implied warranty of marketability or fitness for a particular purpose.

The stated express warranties and the remedy provided for breach thereof, are in lieu of all other liability or obligations of the Company for any damages whatsoever, arising out of or in connection with the delivery, or the inability to use any of its products.

In no event shall the Company be legally liable in the basis of contract, negligence, strict liability in tort, or warranty of any kind for any indirect, special, incidental, consequential or exemplary damages (including but not limited to lost profits).

Without limiting the effect of the preceding sentence, the Company’s maximum liability, if any, shall not exceed the purchase price paid by the Buyer for the product.

11. Patent Disclaimer Unless explicitly stated, no license or immunity under any patent is either granted or implied by the sale of any of our products.

The Company does not warrant that the resale or use of its products delivered will not infringe the claims of any patents, trademarks or copyright covering use of the product itself, or its use in combination with any other products, or its use in the operation of any process. Furthermore, the purchaser assumes all risks of patent, trademark or copyright infringement associated with any such use, combination or operation.

12. Claims and Liabilitya. To the extent permitted by law, all conditions, warranties or obligations

expressed or implied by statute, common law or otherwise are excluded and replaced by the provisions of these conditions.

b. No claim under the Warranty (see Condition 9) will be met if, in the opinion of the Company:

The defect is not due solely to defective materials or manufacture. The Goods have been misused, treated with carelessness, contaminated,

involved in an accident or dealt with in a manner at variance with the Company’s directions.

c. The warranty of condition 9 is specifically limited to the Buyer and does not apply to any other person.

d. The Buyer acknowledges that the Goods will be used for laboratory and research purposes and undertakes not to make them available for human consumption, drugs or household use, directly or indirectly. The Buyer shall indemnify the Company permanently against claims by any third party, arising out of the use or sale of the Goods by the Buyer.

e. All descriptions, representations, illustrations and samples furnished or statements made orally by the Company are given for general information purposes only. Any possible claim can be based only on written specifications agreed at the time of contract.

13. Assignment The Buyer shall not transfer to any other person, any contract to which these

conditions apply or the benefit thereof.

14. Force Majeure Any contract to which these conditions apply is subject to cancellation by the

Company or to any such variation as it might find necessary by reason of strike, lock-out, trade dispute, weather conditions, hostilities, legislation, Acts of God or any cause whatsoever, beyond the control of the Company.

15. Governing Law a. For Goods purchased from Bioline Ltd, the contract shall be governed by and

construed in accordance with the laws of England and Wales and the parties hereby submit to the exclusive jurisdiction of the English courts.

b. For Goods purchased from Bioline GmbH, the contract shall be governed by and construed in accordance with the German civil code (BGB) and the parties hereby submit to the non-exclusive jurisdiction of the German courts.

16. Trademarksa. The Bioline logo is a trademark of Bioline Ltd. The following trademarks have

been registered by Bioline Limited and/or Bioline USA Inc: ACCUZYME, BIOTAQ, BIO-X-ACT, HyperLadder, IMMOLASE, MangoMix,

MangoTaq, MyTaq, TRIsure.b. The following are trademarks registered by other companies: SensiMix™ and SensiFAST™ (Bioline Reagents Ltd), SYBR® Green (Molecular

Probes), PRISM®, StepOne™ (Applied Biosystems™) and ROX™ (Applera Corporation), iCycler®, MyiQ™, iQ™5, Opticon™, Chromo4™, CFX384™ and CFX96™ (Bio-Rad®), LightCycler® and TaqMan® (Roche Molecular Systems Inc), EvaGreen® (Biotium, Inc), HRM™ and Rotor-Gene™ (Qiagen), Mx4000®, Mx3000®, Mx3005P® (Stratagene®), Bioanalyzer (Agilent Technologies Inc), Scorpions™ (Sigma-Aldrich Co.), SmartCycler® (Cepheid®), Quantica® (Techne), Mastercycler® (Eppendorf®), CycleScanner® (Idaho).

c. The Company is an authorized distributor of SensiMix and SensiFAST products, which are manufactured by Bioline Reagents Ltd.

Terms and Conditions of Sale


www.bioline.com

United Kingdom

Bioline Reagents Ltd16 The Edge Business CentreHumber RoadLondon NW2 6EWTel: +44 (0)20 8830 5300Fax: +44 (0)20 8452 2822email: [email protected]

Germany

Bioline GmbHIm Biotechnologiepark, TGZ 2D-14943 LuckenwaldeTel: +49 (0)3371 68 12 29Fax: +49 (0)3371 68 12 44email: [email protected]

Australia

Bioline (Aust) Pty LtdPO Box 122Alexandria NSW 1435Tel: +61 (0)2 9209 4180Fax: +61 (0)2 9209 4763email: [email protected]

USA

Bioline USA Inc.305 Constitution DriveTaunton MA 02780Tel: +1 508 880 8990Fax: +1 508 880 8993Order Toll Free: +1 888 257 5155email: [email protected]

France

Bioline France34 Rue de PonthieuParis 75008Tel: +33 (0)1 42 56 04 40Fax: +33 (0)9 70 06 62 10email: [email protected]

Europe

AustriaVWR International GmbHTel: 01 97 002 0Fax: 01 97 002 [email protected] http://at.vwr.com

BelgiumGentaur Molecular Products Tel: + 32 (0)16 58 90 45Fax: + 32 (0)16 50 90 [email protected] www.gentaur.com

GC Biotech B.V.Tel: +31 172 782 170Fax: +31 172 782 174email: [email protected]

Czech RepublicLAB MARK a.s.Tel:+420 233 335 548Fax: +420 224 311 [email protected]

DenmarkSaveen & Werner ApSTel: +45 86 20 16 16Fax: +45 86 20 15 [email protected] www.swab.dk

FinlandBiotop OyTel:+358 22410099Fax: +358 [email protected]

GreeceaNadrasis Tel: +30 2310 813 122Fax: +30 2310 [email protected]

VARELAS S.A. Tel: +30 210 52 81 900 Fax: +30 210 52 20 926 [email protected]

HungaryCsertex Kft Tel: +36 1 250 4763 Fax: +36 1 250 1257 [email protected]

Izinta Trading Co., Ltd.Tel. No.: +36 1 392-2654...58Fax No.: +36 1 [email protected]

Ireland & Northern IrelandMyBio Ltd.Tel: +353(0)56 7700868Fax: +353(0)56 [email protected]

ItalyAurogene S.R.L.Tel: +39 0683398062Fax: +39 [email protected]

Duotech S.R.L.Tel: +39 02 331 06630Fax: +39 02 331 [email protected]

BioGenerica S.R.L. (Sicily)Tel: +39 (0)95 7916288Fax: +39 (0)95 [email protected]

NorwayNordic BioSite ASTel: +45 800 10301Fax: +46 (0)8 756 [email protected]

The NetherlandsGC Biotech B.V.Tel: +31 172 782 170Fax: +31 172 782 174email: [email protected]

PolandBLIRT S.A.DNA-Gdansk DepartmentTel: +48 587396150Fax: +48 [email protected]

Portugal Citomed Lda.Tel: +351 218421430 Fax: +351 [email protected] www.citomed.pt

RomaniaSC Filara BioMed SRLTel: +40 (0)364 118686Fax: +40 (0)364 [email protected]

SlovakiaLAB MARK a.s.Tel:+420 233 335 548Fax: +420 224 311 [email protected]

SpainEcogen, S.R.L.Tel/Fax: +34 93 4502601 [email protected]

SwedenSaveen & Werner ABTel: +46 40 51 00 00Fax: +46 40 16 45 [email protected]

Nordic BioSite ABTel: +46 (0)8 544 433 40Fax: +46 (0)8 756 94 [email protected]

SwitzerlandBiolabo Scientific Instruments SATel: +41 21 948 77 15Fax: +41 21 948 78 [email protected]

TurkeyArdi Medikal Ltd. Şti.Tel: +90 216 443 05 44Fax: +90 216 443 01 [email protected]

Africa

South AfricaCeltic Molecular Diagnostics (Pty) Ltd.Tel: +27 21 7627703Fax: +27 21 [email protected]

EgyptAmerican Trade Co.Tel: +02-03-4275671Fax: +02-03-4247591www.americantrade.com.eg

MoroccoBiotechnics SolutionTel: +212 5 22 83 31 73Fax: + 212 5 22 86 03 [email protected]

TunisiaBIOLOMEDICTel: +216 71 903 824Fax: +216 71 903 [email protected]

Middle East

IsraelOrigolab Ltd.Tel: +972 2 5669285Fax: +972 2 [email protected]

Saudi Arabia BiomedicaTel: +9661 456 0906Fax: +9661 494 [email protected]

UAEHexa Healthcare Equipment Trading L.L.C.Tel: +971 4 267 2501Fax: +971 4 267 [email protected]

Bioline products are available worldwide through our network of distributors. Each distributor has been

carefully selected for their ability to deal with our product line and to offer a high level of customer support.

Bioline Distributors

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