Proceeding of Cellular and Molecular Biology...

In the name of God

the Merciful, the Compassionate

20th

National and 8th

International Congress of Biology,

22-24 August, University of Maragheh, Iran

The congress was held as four distinct confrences included:

Applied, Animal, Cellular and Molecular, and Plant Biology Confrences

Proceeding of

Cellular and Molecular Biology

Conference

Welcome Message of Congress President

In the name of God

The history of biology goes back to long time ago, about 3.8 billion years, when the first living

creatures began to exist on the earth as amoebas. Although biology was confirmed as an independent

discipline in the 19th

century, it actually originated in ancient medical science in Mesopotamia, China,

India, and Egypt. Nevertheless, modern biology and its tendency to study the nature go back to ancient

Greece. During the Renaissance and early modern era, biological thoughts underwent major changes

due to development of an inclination toward empiricism and discovering many types of new living

creatures.

It is a pleasure for us to welcome eminent scholars and researchers to the 20th

National and 8th

International Biology Congress in Iran, Maragheh. Maragheh encompasses the most comprehensive of

all scientific, cultural and artistic treasures. Maragheh reminds us the memory of a "university as wide

as a city" culture because it first presented it to the history and world of science. The development of

the City- University of Maragheh, in its historical memory, commemorates the first girls' school (the

dynasty of the Ilkhani), is undoubtedly based on the revival and stabilization of scientific, cultural and

artistic School of Maragheh.

University of Maragheh is proud to hold this glorious scientific congress by the presence of honorable

professors, researchers, students, teachers, and other guests interested in biology.

Wishing you all a heartfelt welcome.

Mohammad-Ali Lotfullahi Yaghin

The Massage of Congress Chairman

In the name of Allah

We are very glad that the Iran’s 20 th national and 8th international Congress of biology 2018 took

place in the beautiful city of Maraghe with the estimable endeavor and cooperation of chancellor and

faculty members of the University of Maragheh and the managing council of Iranian society of

biology.

This gathering was an opportunity for all Iranian biologists inside and outside the borders of Iran to

obtain the basis of more progresses in this zone of mankind knowledge, with interchanging their

information and achievements together and with scientists from different countries of the world like

Italy, Canada, Turkey, Armenia and...

The society of biology is honored for obtaining the conditions for this affair, and is thankful to

Maraghe university, the academic staff at department of biology and other collaborators of that

university for their heartily host.

The endeavor of the scientific and administrative committees in this congress in creating scientific and

heartily atmosphere during the period of celebration is definitely praiseworthy.

I deem it necessary for myself to heartily appreciate the endeavor of the respectful directorship, vice

chancellors and specially the dear students of Maraghe university.

And to wish grace and success for all my valuable colleagues in Iranian society of biology’s executive

committee who endeavored in both administration and diplomacies of this congress.

And hope to see all of the participants from all over the world in the next congress.

Mohammad Nabiuni

Executive Board

President

Prof. Mohammad-Ali Lotfollahi Yaghin (Chancellor of the University of Maragheh)

Chairman

Prof. Mohammad Nabiuni (President of the Iranian Biology Society)

Secretary General

Dr. Saleh Shahabivand (University of Maragheh)

Vice-Chairman

Prof. Nader Chaparzadeh (Azarbaijan Shahid Madani University)

Executive Program Committee

Dr. Ahmad Aghaee (General Executive Secretary of Congress, University of Maragheh)

Dr. Reza Masoumi Jahandizi (Accommodation, University of Maragheh)

Dr. Raheleh Majdani (Registration, University of Maragheh)

Dr. Parisa Fathirezaei (Reception,University of Maragheh)

Dr. Mohammad Moshtary (Public Relations)

Dr. Reza Mohammadzadeh (International Affairs, University of Maragheh)

Dr. Mehdi Djahangiri (Secretariat Affairs, University of Maragheh)

Scientific Program Committee

Dr. Farrokh Karimi (General Scientific Secretary of Congress, University of Maragheh)

Dr. Farshad Darvishi (General Scientific Secretary of Congress, University of

Maragheh)

Dr. Parisa Fathirezaei (Scientific Secretary of Cellular and Molecular Biology

Conference, University of Maragheh)

Dr. Reza Masoumi Jahandizi (Scientific Secretary of Cellular and Molecular Biology

Conference, University of Maragheh)

Dr. Leila Zarandi-Miandoab (Proceeding Collection, Azarbaijan Shahid Madani

University)

Dr. Mehdi Djahangiri (Proceeding Collection, University of Maragheh)

Dr. Younes Aftabi (Abstracts Edition)

Zahra Khoshkam (Abstracts Edition)

Scientific Committee

Afshar Mohammadian Mansour

(University of Guilan)

Hamidiyeh Amir-Ali

(Tehran University of Medical Sciences)

Motallebi Mostafa

(NIGEB)

Aghaei Ahmad

(University of Maragheh)

Hasanpour Halimeh

(Aerospace Research Institute)

Mousavi Gargari Mir Latif

(Shahed University)

Aminzadeh Saeid

(National Institute of Genetic Engineering

and Biotechnology)

Hejazi Mohammad-Saeed

(Tabriz University of Medical Sciences)

Mousavi Movahedi Ali-Akbar

(University of Tehran)

Bahaedini Aminolah

(Shiraz University)

Hoseinpour Feizi Mohammad-Ali

(University of Tabriz)

Nabiuni Mohammad

(Kharazmi University)

Baharvand Hosein

(Royan Institute)

Hosseinzadeh Colagar Abasalt

(University of Mazandaran)

Naderimanesh Hosein

(Tarbiat Modares University)

Bahrami Mohammad-Kazem


Iranbakhsh Alireza

(Islamic Azad University,Tehran)

Nejad Falatoury Moghadam

Atiye

(Iranian Research Institute of Plant

Protection)

Barzegari Amir-Abbas


Karimi Farokh


Omidi Yadolah

(Tabriz University of Medical

Sciences)

Chaparzadeh Nader

(Azarbaijan Shahid Madani University)

Keykhosravi Alireza

(Hakim Sabzevari University)

Rastegar Pouyani Nasrolah

(Razi University)

Chehregani Rad Abdol-Karim

(Buali Sina University)

Khajeh Khosro


Roayaei Ardakani Mohammad

(Shahid Chamran University of

Ahvaz)

Darvishi Farshad


Maassoumi Ali-Asghar

(Research Institute of Forests and Rangelands)

Sari Alireza


Ebrahimzadeh Maboud Hasan


Majd Ahmad


Sariri Reyhaneh

(University of Guilan)

Ehsanpour Ali-Akbar

(University of Isfahan)

Majdani Raheleh


Sepehri Sepideh


Ejtehadi Hamid

(Ferdowsi University of Mashhad )

Malboubi Mohammad-Ali

(National Institute of Genetic Engineering and

Biotechnology)

Shahabivand Saleh


Eslimi Esfahani Delaram


Masoumi Jahandizi Reza


Shariati Mansour

(University of Isfahan)

Farid Alaei Gholam-Reza

(Maragheh University of Medical

Sciences)

Mehmannavaz Usef

(Maragheh Branch, Islamic Azad University)

Tanomand Asghar

(Maragheh Faculty of Medical

Sciences)

Fathirezaei Parisa


Mobasheri Hamid


Valilou Mohammad-Reza

(Maragheh Branch, Islamic Azad

University)

Feizabadi Mohammad-Mehdi

(Tehran University of Medical Sciences)

Moghadam Matin Maryam

(Ferdowsi University of Mashhad)

Zamani Mohammad-Reza

(NIGEB)

Ghareyazie Behzad

(Agricultural Biotechnology Research

Institute of Iran)

Mohajel Kazemi Elham

(University of Tabriz)

Zarandi Miandoab Leila

(Azarbaijan Shahid Madani

University)

Golkari Saber

(Dryland Agricultural Research Institute)

Mohammadzadeh Reza


Zare maivan Hassan


Gourabi Hamid

(Royan Institute)

Mohsen Sharifi


Zeinali Sirous

(Pasteur Institute of Iran)

Referees

Afshar Mohamadian Mansour

Akhavan Azadeh

Akmali Vahid

Alavi-Yeganeh Mohammad

Sadegh

Aliahmadi Atousa Alipanah Helen Alivand Mohammadreza Amirahmadi Atefeh

Amiri Gholamreza Amiri Hamzeh Ansarihadipour Hadi Arzani Nima

Asadollahi Mohammadali Ashengroph Morahem Ashrafi Osalou Mostafa Askari Nahid

Assadian Narenji Somayeh Attaran Fariman Gilan Badui Arastu Bagheri Zainab

Barshan Tashnizi Mohammad Barzegari Amir Abbas Basir Zahra Bathai Zahra

Chaparzadeh Nader Cheniany Monireh Dadfar Fereshteh Daihassani Behrokh

Daneshvar Abolfazl Darvishi Farshad Davoodi Parisa Divsalar Adeleh

Eagderi Soheil Ebadi Mostafa Ebrahimi Mohaddese Ebrahimi Vosta Soheila

Ebrahimi Raheleh Ehsanpour Ali Akbar Elikaei Amaneh Enteshari Shokofeh

Esfahani Kasra Esfandiari Neda Falatoury Atiye Farhad Talebi Ahmad

Farhoudi Roozbeh Farzamisepehr Mozhgan Fathi Rezaei Parisa Forghani Amir Hossein

Gashmardi Noushin Gavzan Hakimeh Ghaffarian Sara Ghanbari Sajad

Ghasemi Omran Vali Ollah Ghassemi Farangis Ghezelbash Gholamreza Gholamhosseini Ali

Gholami Parviz Gholipour Abbas Gholizadeh Mohammad Ghorani Mohammadreza

Ghoshooni Hasan Gohari Gholamreza Habibi Ghader Hajipour Orkideh

Hajipour Nasser Hajrasouliha Shadi Hashemipetroudi

SeyedHamidreza

Hasanshahi Mehdi

Hatamnia Ali Asghar Hekmat Azadeh Hosainzadegan Hassan Imani Mahdi

Jafari Yaser Jalali Amir Jalalvand Fateme Jamalomidi Masoomeh

Javanbakht Hossein Joudi Leila Kameli Maryam Karamiani Rasoul

Karimi Zohreh Karimi Shahri Mahmoud Reza Katiraee Farzad Kavyanifard Amirarsalan

Kelij Sedigheh Keshtmand Zahra Keykhosravi Alireza Khakpaay Roghayye

Khalaji Valiyollah Kohan-Baghkheirati Eisa Koochaknejad Emad Loghmani Mehran

MahmoodzadehHossein Hamideh Mahmoudi Fariba Mahmoudi Otaghvari Arman Majdani Raheleh

Maleki Masoume Malekzadeh Parviz Masoomi Jahandizi Reza Mehraban Pooyan

Mianabadi Manijeh Moghimi Hamid Mohadjerani Maryam Mohajel Kazemi Elham

Mohajjel Shoja Hanieh Mohamadi Ghasem Mohamadzadeh Reza Masoumi Seyed Mohammad

Mohammadi Parisa Mohammadi Hasem Mohammadi Habibollah Mobini-Dehkordi Mohsen

Mohseni Mojtaba Mohtadi Ahmad Mollania Nasrin Momeni Lida

Monsef Shokri Maryam Motamedi Hosein Motamedi Javad Mousavi Fateme

Mozafari Hossein Nazari Farzad Negaresh Kazem Nejadhabibvash Fatemeh

Niroomand Azadeh Nofouzi Katayoon Norizadeh Tazehkand Mostafa Nouri Sahar

Panahi Bahman Parishani Mohammad Reza Pazhang Mohamad Pazhang Yaghub

Pouresmaeil Vahid Raeghi Saber Maryam Rahimi Elham Rajabbeigi

Ramak Parvin Rasouli Sohrab Rastgar Somayeh Razavi Mehdii

Razavi Khadijeh Rezaei Tavabe Kamran Roudbari Fatemeh Roumi Vahid

Sadat Hosseini Afrouz Sadat Atri Maliheh Sadeghi Parvin Sadeghi Dehcheshmeh Rasoul

Sadeghi Akram Sajedi Reza Salavatifar Maryam Salehi-Eskandari Behrooz

Salehi- Lisar Seyed Yahya Sarafraz Ardakani MohammadReza Saraygord-Afshari Neda Sari Alireza

Shafaie Sepideh Shafiei Rasoul Shahbazi Samira Shahbazi Hadis

Shahbazi Parisa Shakeri Shahryar Shakhsi-Niaei Mostafa Shamili Mansooreh

Sharifi-Tehrani Majid Shaykh-Baygloo Nima Shirzadian Saeed Siasi Elham

Simaei Mehdi Soltanzadeh Hossein Tafrihi Mjiad Talebi Mehrdar Mahboobeh

Tanomand Asghar Teravati Ali Thidi Fatemeh Toghranegar Zohreh

Vahdatpour Tohid Valipour Masoumeh Yaghoobi Hanif Yaghubi Hashem

Yari Siamak Yazdanbakhsh Nima Zadeh Hosseingholi Elaheh Zamani Abbas

Zarandi- Miandoab Leila Zarrini Gholamreza Zeinalzadeh-Tabrizi Hossein Zolghadri Samaneh

Executive Committee Abbasi Robabeh Ghoutaslu Armita Naderahmadian Aylar

Abdoljabari Mahsa Habibeh Asgari Naghizadeh Parvin

Abedinzadeh Ali Hajian Mahboubeh Najafi Zahra

Adraki Fahimeh Hasanzadeh Saeed Najm Amir

Afkhami Saber Hassanzadeh Mona Narj-Abadian Alireza

Ahadi Adib Hosseini Amir Navaei Anahita

Ahmadi Gelavizh Hosseini Firouz Nemati Faezeh

Ahmadiyeh Amir Hossein Imamzadeh Rohallah Pooya Pegah

Ahmadpour Mohammad Isma'ilian Sara Radan Sahar

Akbari Tara Jaberi Mohsen Rahim fam Rasoul

Alizadeh Saeid Jalilian Elham Rahimi Samin

Amraei Mahtab Kalhor Narges Rahmani Robabeh

Artesh Rezaei Hosein Karbala'i Mehdi Mohammad Rezaie Ali

At'hayi Masoumeh Kermani Elham Rezaie Hanieh

Bandali Amir Hossein Khani Fatemeh Rousta Zainab

Barzegari Amir Abbas Kheirallahi Armin Sadeghi Moslem

Basak Nastaran Kheirallahi Sina Sardari Maryam

Bashiri Saba Khordlou Mona Sarmasti Siavash

Bidar Mohsen Mahdavi Javad Shaghaghi Neda

Danesh Maraghi Amir Majdani Raheleh Sharifi Motlagh Behdad

Darya JavadRashid Mansour Dehghani Sharifi Motlagh Behdad

Dehghan Yaghoub Mansouri Farhad Sharifi Motlagh Tanaz

Dinarvand Behnoosh Marami Rahele Soltani Elias

Djahangiri Mehdi Marzoukian Kimia Soltani Nima

Fathi Leila Moaafi Fatemeh Tafaghodi Behzad

Fathi Rezaei Parish Moghaddami Noushin Taghavi Hamid

Feizi Parisa Moghimi Fam Javad Tamkinvash Solaleh

Fonudi Ehsan Mohaddesi Javad Tanha Mehdi

Fouladi Maryam Mohammadi Arezoo Vand Jalili Mohammad

Ghaeli Bahman Mohammadi Shiva Yari Behnia

Ghasemi Hanieh Mohammadpour Samaneh Yavari Khadijah

Ghasemi Toraj Mohammadzadeh Reza Yousefi Marzieh

Ghasemzadeh Mahin Moshtary Mohammad

Ghasemzadeh Samaneh Mossadegh Arefeh

Sponsor Acknowledgement

The organizing committee sincerly thanks the support of the

following sponsors

Ministry of Science, Research and Technology, I.R. Iran

Iran National Science Foundation (INSF), I.R. Iran

ECO Science Foundation (ECOSF)

UNESCO Chair in Life Sciences, Armenia

Maragheh Office, Department of Environment, I.R. Iran

Dryland Agricultural Research Institute, Maragheh, I.R. Iran

Maragheh University of Medical Sciences, I.R. Iran

Research Institute for Astronomy and Astrophysics of Maragha, I.R. Iran

Islamic World Science Citation Center (ISC)

Regional Information Center for Science and Technology (RICeST), I.R. Iran

Islamic City Council of Maragheh, I.R. Iran

Maragheh Municipality, I.R. Iran

Maragheh Grand Hotel, I.R. Iran

Milad Noor Ofogh Company, I.R. Iran

Maragheh Athar Flour (Ard-e-Athar) Company, I.R. Iran

Kaveh Soda Company, I.R. Iran

1

Congress Plenary Invited Lectures

Prof. Khosrow Adeli (University of Torento, Canada)

RNA regulatory network in lipid metabolism: critical rols of micro RNAs and RNA

granuls

Prof. Sinerik N. Ayrapetian (Head of UNESCO Chair in Life Science, Armenia)

The quantum-mechanical nature of cell signaling system

Prof. Mohammad Ghannadi-Maragheh (Institute of Nuclear Science and Tecnology,

I.R. Iran)

Application of nuclear science and tecnology in biology and medicine

Prof. Ali Akbar Moosavi-Movahedi (University of Tehran, I.R. Iran)

Biomimetics and lifestyle

Prof. Luciano Sasso (Sapienza University of Rome, Italy)

Pharmacological applications of modulators of oxidative stress

2

Lectures

3

Cellular and Molecular Biology Conference Invited

Lectures Prof. Mohammad-Mehdi Feizabadi (Tehran University of Medical Sciences) Antibiotic

resistance of Gram-negative bacteria: A serious threat to global public health

Prof. Ali Hatef Salmanian (National Institute of Genetic Engineering and

Biotechnology) Revers vaccinology and a breakthrough in vaccine development

Prof. Abasalt Hosseinzadeh Colagar (University of Mazandaran)

Down regulation of WT1 gene transcript via stabilization of promoter G-quadruplexes

Assoc. Prof. Hamid Mobasheri (University of Tehran) Electromagnetic communication

between isolated cells, possible application in regenerative medicine

4

Lectures Contents

Role of β-diketonate group of curcumin on bilateral actions

of it in presencedifferent concentrations of catalase............... 5

Investigation of the hypoglycemic, antioxidant and anti-

bacterial effects of the waste of four rice varieties in Gilan

province .................................................................................. 5

Effects of magnetic fields on carbohydrate content in two

specious of Almond ................................................................ 6

The effect of gallium 67 on the serum level of calcium ......... 6

Spectroscopic investigation of groove binding interaction of

Fe3O4@CaAl LDH@L-Dopa with Calf Thymus DNA .......... 7

Study of betatrophin effects on Wnt signaling pathway ......... 7

DNA breaking and antibacterial effect of Metoclopramide ... 8

Homology modeling and site-directed mutagenesis of

chitinase fromStreptomyces griseolosporeus ......................... 8

Investigation of the NF-KB signaling pathway in whole

blood samples of patients with Multiple Sclerosis ................. 9

The G-quadruplex structure of COX-2 gene promoter: a novel

structure to inhibit the COX-2 ................................................ 9

Loading Jellyfish venom C-CfTXA-STxB chimeric antigen

in PLA-PEG-PLA copolymers with a tri-block configuration

.............................................................................................. 10

The effect of soluble form of VEGF8-109 on vein endothelial

cells ...................................................................................... 10

Identifying ovarian cancer micro RNA bio-Markers using a

sequential wrapper method ................................................... 11

Investigation of bacteriophage infections of potato soft-rot

causal bacteria in potato farms of Tabriz ............................. 11

Isolation of multiple drug-resistant genes on exfoliative toxin

B-encoding plasmid in Staphylococcus aureus obtained from

skin infection samples .......................................................... 12

The evaluation of antibiotic resistance pattern and frequency

of extended-spectrum beta-lactamases among clinical isolates

of Pseudomonas aeruginosa isolated from inpatients of Imam

Khomeini hospital, Kermanshah, Iran .................................. 12

An introduction to a plastic consumer microbial isolate from

soil samples of Iran .............................................................. 13

Isolation of Shewanella spp. from sediments of the Caspian

Sea for electricity generation from synthetic wastewater in

microbial fuel cells ............................................................... 13

Investigation of Bacillus licheniformis OT9 isolated from the

Tabriz refinery soil in microbial enhanced oil recovery ....... 14

5

Role of β-diketonate group of curcumin on

bilateral actions of it in presencedifferent

concentrations of catalase

Reza Yekta, Samaneh Rashtbari, Gholamreza Dehghan*

Department of Biology, Faculty of Natural Sciences, University of Tabriz, Tabriz, Iran

* Corresponding author: [email protected]

Curcumin as food additive extensively is being used

around the world daily. In the present study, we

investigated the role of β-diketonate group of curcumin on

its actions in presence different concentrations of catalase

using different spectroscopic and computational methods.

Experimental and theoretical studies revealed that by

rising concentrations of catalase, the enzymes were being

protected by interaction other catalases via β-sheet

domains and increases their stabilities. Following that,

curcumin couldn’t inhibit catalase in higher

concentrations, while in lower concentrations of catalase,

curcumin can strongly inhibit this enzyme. However, by

changing characteristic and structure of β-diketonate group

of curcumin with binding Mn ion, curcumin lost these

bilateral actions in presence different limited

concentrations of catalase. It is suggested that interactions

of catalases in higher concentrations prevented binding

curcumin to the enzyme appropriately, while in lower

concentrations of catalase, they cannot preserve

themselves from toxicity effects of curcumin.

Keywords: Catalase, Curcumin, β-diketonate group,

Inhibition, Activation

Investigation of the hypoglycemic, antioxidant

and anti-bacterial effects of the waste of four

rice varieties in Gilan province

Maryam Hemmati, Moslem Afsharnezhad, S. Shirin Shahangian,

Reyhaneh Sariri* Department of Biology, Faculty of Sciences, University of Guilan, Rasht,

Iran


The growing interest on the replacement of synthetic

antioxidants with natural ones has directed many

researches toward the plant-derived raw materials. The

special attention is focused on inexpensive and residual

sources from food agricultural industries. In the present

study, the antioxidant, anti-bacterial and anti-amylase

properties of the acetone extract of the bran of four rice

varieties of Guilan province, including Hashemi, Tarom,

Neda and Dasht were investigated. Anti-amylase activity

was evaluated by Bernfeld method using DNS reagent.

DPPH and FRAP methods were used to determine the

antioxidant activity of the extract. Also, total phenol and

flavonoid content and anthocyanin were also estimated.

Folin and ciocalteu methods were used to determine the

total phenol. Colorimetric aluminium chloride methods

were used to determine flavonoid and mita et al methods

were used to determine anthocyanin. Antibacterial

properties were studied by disk diffusion method. The

results showed that the highest ferric reducing ability

belongs to acetone extract of Dasht wastes (1/36 mM Fe

(II)/g DW) and the highest percentage of free radical

inhibitory belong to the acetone extract of Neda variety.

Also, the highest amounts of phenol (0.79 mg GAE/g DW)

flavonoids (55.49 µg QE/g DW) and anthocyanins

(0.016mg/g DW) belong to the acetone extract of Dasht

variety. Tarom variety showed the most efficient anti-

bacterial activity on Escherichia coli and Micrococcus

luteus bacteria, whereas Neda and Dasht varieties showed

anti-bacterial potential against Pseudomonas Aeruginosa

and on Staphylococcus aureus.The extracts were prepared

using mixed solvent possessed hypoglycemic activity.

Keywords: Rice waste, Hypoglycemic, Antioxidant,

Antibacterial

.

mailto:[email protected]

6

Effects of magnetic fields on carbohydrate

content in two specious of Almond

Fatemeh Abdollahi1*, Hamzeh Amiri1, Vahid Niknam2, Faezeh Ghanati3,

Kazem Mahdigholi2 1 Department of Biology, Faculty of Science, Lorestan University, Iran 2 Department of Plant Physiology, Faculty of Biology, University of

Tehran, Iran 3 Department of Plant Physiology, Scientific Boards, Tarbiat Modarres

University, Iran


During the past decade considerable evidence has been

accumulated with regard to the biological effects, both in

vivo and in vitro, of extremely low frequency electric and

magnetic fields, such as those originating from

residentially proximate power lines, household electrical

wiring and diagnostic apparatus and therapy devices. Also,

during the evolution process, all living organisms

experienced the action of the Earth's magnetic field, which

is a natural component of their environment. Previously

many scientists believed that permanent magnetic fields

are not biologically active. However, the results obtained

have revealed the high sensitivity of plants to permanent

magnetic fields. In the present research, seeds of Almond

(two specious of Amygdalus scoparia and A. eburnea)

were incubated in sterile conditions. Unique seeds were

selected and divided to control and treatment groups. The

treatment plant groups were exposed to a 10 mT static

magnetic field for four days, each 5 hours and then both

the treated seeds and the control one were harvested,

frozen with liquid N2 and used for biochemical

measurements. Exposure of seeds of almond to the static

magnetic field decreased fresh weight and dry weight of

seeds and increased water content. Magnetic fields also

decreased polysaccharide content and increased

oligosaccharides and soluble sugars in plant.

Keywords: Polysaccharide, Oligosaccharides, Magnetic

fields, Soluble sugars, Water content

The effect of gallium 67 on the serum level of

calcium

Kourosh Bamdad, Fereshteh Dadfar*, Majid Parak

Departement of Biology, Faculty of sceinces, Payame Noor University,

Iran


With of the advancement of science, the ionizing radiation

were used in medical applications such as radiology, CT

and nuclear medicine. Gallium 67 is used in nuclear

medicine imaging to detect soft tissue tumors and

inflammatory diseases. However, it should be considered

the dangers of radiation of it's because it can have the side

effects on the organs including the endocrine system and

subsequent serum blood changes. 60 male rat in the range

of 250-300 g were divided to two equal groups randomly.

The first group was considered as control. The second

group received 0.3 mg gallium 67 by Intraperitoneal

injection. After one week, it was taken 2 cc blood clots

from the heart all of them for serological studies. Data

were analyzed by statistical t-Test. The result was showed

the significant difference in the levels of calcium in

gallium 67 group with compare contol group. Therefore, it

can be concluded that gallium 67 may have the effect on

the level of calcium blood with the effect of hormones

involved in regulating calcium levels including

parathormone hormone.

Keywords: Gallium 67, Calcium, Serum changes



7

Spectroscopic investigation of groove binding

interaction of Fe3O4@CaAl LDH@L-Dopa

with Calf Thymus DNA

Mahtab Razlansari1*, Nahid Shahabadi2,3 1 Institute of Nano Science and Nano Technology, Razi University, Kermanshah, Iran 2 Medical Biology Research Center (MBRC), University of Medical

Sciences, Kermanshah, Iran 3 Faculty of Chemistry, Razi University, Kermanshah, Iran


The main goal of this study is the evaluation of groove

binding interaction of Fe3O4@CaAl LDH@L-Dopa with

calf thymus DNA (CT-DNA). The magnetic nanoparticles

were prepared by a chemical co-precipitation method and

the surface of the Fe3O4 nanoparticles was coated with

CaAl layered double hydroxides (CaAl LDHs). This core-

shell nanostructure was used as a carrier for the

antiparkinsonian drug “L-Dopa” to achieve the drug

delivery system with suitable properties for biological

applications. The structural features of Fe3O4@CaAl

LDH@L-Dopa were evaluated using various techniques

like XRD, FT-IR, TEM, and SEM. According to the

obtained results from the physicochemical analysis, the

core-shell structure of Fe3O4@CaAl LDH@L-Dopa with

about 120 nm average size, was confirmed. Also, the

interaction of Fe3O4@CaAl LDH@L-Dopa with CT-

DNA has been studied using, UV-Visible spectroscopy,

viscosity, circular dichroism (CD) and fluorescence

spectroscopy techniques. The results of investigations

clearly demonstrated that Fe3O4@CaAl LDH@L-Dopa

has interacted via groove binding to CT-DNA.

Keywords: L-Dopa, Layered double hydroxide, Magnetic

nanocarrier, Drug delivery, Drug release, Cell culture

Study of betatrophin effects on Wnt signaling

pathway

Nastaran Monzavi1*, Seyed Jalal Zargar1, Nematolah Gheibi2

1 Department of Cell & Molecular Biology, School of Biology, College of Science, University of Tehran, Tehran, Iran 2 Department of Biothecnoligy, Faculty of Paramedicals, Qazvin

University of medical sciences, Iran * Corresponding author: [email protected]

Wnt signaling has fundamental roles in survival, cell

proliferation, cell fate and any disorder in it, may lead to

numerous malignancies including HepatoCellular

Carcinoma (HCC). Nearly 95% of diagnosed HCC cases,

showed aberrance in the Wnt signaling pathway. Two key

genes in this pathway are WIF1 and β-catenin, the

subnormal function of them can cause the activation of the

Wnt pathway and subsequently, cell proliferation and

carcinogenesis occur. So any substance which can inhibit

this pathway is considered valuable. Betatrophin is a

newly identified protein which is upregulated in HCC. In

this study, we investigated the effect of this protein on the

expression level of WIF1 and β catenin. Recombinant

betatrophin was produced in the BL21 system and the

purification of it was performed by Ni-NTA

chromatography. After treatment of HepG2 cell lines with

different concentrations of betatrophin, total cell RNA was

extracted and Real-Time PCR was conducted in order to

analyze the mRNA levels of WIF-1 and β-catenin.

Betatrophin could increase the expression of WIF-1 up to

1.6 fold and decrease the expression of β-catenin by up to

0.4 fold. WIF-1 is the antagonist of Wnt proteins and it can

inhibit the Wnt pathway, down-regulation of it has been

shown in many malignancies and at the other hand

accumulation of β-catenin as well, has been observed in

tumors. So these results suggest that betatrophin by

increasing and decreasing of WIF1 and β catenin

respectively is capable to inhibit the Wnt signaling

pathway and it can be used as an anti-cancer drug.

Keywords: Wnt signaling pathway, Betatrophin, WIF-1,

β-catenin, Carcinogenesis



8

DNA breaking and antibacterial effect of

Metoclopramide

Mostafa Norizadeh Tazehkand*

Department of Pharmaceutical Biotechnology, Faculty of Pharmacy, Bulent Ecevit University, Zonguldak, Turkey


Metoclopramide is using for stomach and esophageal

problems. It is frequently used to treat and

prevent nausea and vomiting, to help withvacantof the

stomach in people with delayed stomach emptying, and to

help with gastroesophageal reflux disease. It is also used to

treat migraine headaches.This study was aimed to the

investigation of antimicrobial and genotoxic effects of

metoclopramide using different test systems. The

Minimum inhibitory concentration (MIC) and minimum

bactericidal concentration (MBC) was determined by

dilution assay using different concentrations of

metoclopramide against 2 bacteria (Bacillus subtilis as a

gram-positive bacterial strainand Pseudomonas aeruginosa

as a gram-negative bacterial strain). Disk diffusion assay

were prepared under sterile conditions disks of drugs

(three repeat for each concentration) containing three

different doses (50, 100 and 150 µg) were prepared. The

Mueller Hinton agar plates were incubated at 37oC for 12

hours. In addition, DNA breaking effects of

metoclopramide were analyzed on pET22b plasmid. MIC

values of metoclopramideagainst Pseudomonas

aeruginosa was 2 mg/ml, and MBC values was 5.4 mg/ml

and MIC values of metoclopramideagainst Bacillus subtilis

was 0.800 mg/ml, and MBC values was 5.4 mg/ml. The

results obtained from disk diffusion assay supported that

the metoclopramidehas not anti-bacterial effect against

Pseudomonas aeruginosa but has low antibacterial effect

on Bacillus subtilis. In this research metoclopramide not

demonstrated a cleavage activity on pET22b plasmid

DNA. These findings showed that metoclopramide did not

have significantly genotoxic and antimicrobial effects (on

normal bacterial flora). It can be said that metoclopramide

does not have a risk for humans.

Keywords: Antibacterial effects, Metoclopramide,

Pseudomonas aeruginosa, Bacillus subtilis

Homology modeling and site-directed

mutagenesis of chitinase fromStreptomyces

griseolosporeus

Vajiheh Eskandari*

Department of Biology, University of Zanjan, Zanjan, Iran * Corresponding author: [email protected]

Chitin is the second most abundant biopolymer on the

earth after cellulose. Chitinases are glycosyl hydrolases

that catalyze the conversion of chitin biopolymer to low

molecular weight chitooligomers, have a wide variety of

biotechnological and industrial applications. Template

crystal structure of chitinase from Streptomyces

griseus (PDB ID: 1wvu) was used for homology modeling

of the enzyme (UniProt ID:A0A0D0Q8E2) using Modeler

software. The model was loop refined and was validated

using RMSD, ProSA, and PROCHECK. The refined

model was submitted to the Protein Model Data Base. The

docking was carried out to elucidate the interaction

energies of amino acid residues with the chitin ligand,

obtained from the ChemSpider database. To enhance the

binding of chitin with the enzyme, mutation studies were

carried out by replacing Thr14 as it had a less interaction

energy. Out of 10 mutants were selected using the

PoPMuSiC server. The favorable mutant for binding of

chitin was chosen based on RMSD and RMSF of MD

simulations. Thus, modeling chitinase would aid in the

detailed understanding of its structural properties and

mutation studies would help in improving the enzyme

efficiency

Keywords: Chitinase, Homology modeling, Docking,

Mutation and molecular dynamics


https://en.wikipedia.org/wiki/Nausea

https://en.wikipedia.org/wiki/Vomiting

https://en.wikipedia.org/wiki/Gastric_emptying

https://en.wikipedia.org/wiki/Gastric_emptying

https://en.wikipedia.org/wiki/Gastroparesis

https://en.wikipedia.org/wiki/Gastroesophageal_reflux_disease

https://en.wikipedia.org/wiki/Migraine


http://www.uniprot.org/uniprot/A0A0D0Q8E2

9

Investigation of the NF-KB signaling pathway

in whole blood samples of patients with

Multiple Sclerosis

Seyed Alireza Mesbah-Namin1*, Hamid Zahednasab2 1 Department of Clinical Biochemistry, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran. 2 Department of Biochemistry, Institute of Biochemistry and Biophysics,

University of Tehran, Tehran, Iran * Corresponding author: [email protected]

The present research is on the signaling pathways of NF-

KB, on nearly 200 DNA blood samples of the patients

with Multiple Sclerosis (MS), focused on a set of genetic

and epigenetic alterations on promoters of that genes,

using PCR-RFLP, sequencing, andmethylation-specific

PCR. Autoimmune MS disease is characterized by

demyelination due to immune reactions against the myelin

sheath, and NF-KB is an inducible transcription factor in

lymphocytes and the immune system, presents in the

cytoplasm in association with inhibitory proteins (IkB),

involved in the regulation of major inflammatory

responses. NF-kB signaling pathways are initiated through

extracellular stimuli and following activation of the IκB

kinase (IKK) complex, signal leads to the degradation of

IkB and the resultant release and translocation of the

relevant NF-kB into the nucleus for transcriptional

activation. Most of MS patients had relapsing/remitting

status and healthy control individuals matched based on

sex and age. The results of these studies revealed there are

no significant differences in the frequency of the -94

ins/del ATTG polymorphism in the promoter of NF-KB

gene but the genotype frequency of IkBα -881 A/G was

significantly higher in the MS patients than in the controls.

Methylation pattern of IKK gene promoter was also

significantly correlated with the disease susceptibility. The

role of NF-kB in autoimmune diseases is undeniable and

the association between IkB promoter gene

polymorphisms/ hyper-methylation of related IKK

promoter gene and susceptibility of MS disease are partly

due to different transcriptional activities and the activation

of NF-kB.

Keywords: Multiple sclerosis, Transcription factor, NF-

KB, Polymorphism, Epigenetic

The G-quadruplex structure of COX-2 gene

promoter: a novel structure to inhibit the

COX-2

Tahereh Zahedi1, Abasalt Hosseinzadeh Colagar1*, Raoof Jahan-Bakhsh 2,

Habibollah Mahmoodzadeh3 1 Department of Molecular and Cell Biology, Faculty of Basic Science,

University of Mazandaran

2 Department Analytical Chemistry, Faculty of Chemistry, University of Mazandaran

3 Department of Surgical Oncology, Cancer Institute, Imam Khomeini

Hospital Complex, Tehran University of Medical Sciences * Corresponding author:[email protected], [email protected]

Overexpression of COX-2 gene, an inducible

inflammatory gene, has a special role to begin the

tumorigenesis and angiogenesis and to increase of

colorectal tumor polyps. The other studies have shown the

potential of G-quadruplex structure formation of

oncogenes promoter. So the formation of a G-quadruplex

structure on promoter region could be used as a strategy to

control cancer. One region of a COX-2 promoter which

has the G-quadruplex formation potential has been

identified by QGRS online software. The formation and

stability of the G-quadruplex structure of this sequence

have been assayed in the condition of 0-2 μM of G-

quadruplex stabilizer vs of control negative by PCR Stop

method which assayed the intensity of bands on PAGE

15%. The potential sequence which has been founded by

QGRS is a 29bp sequence and its G-score is 32 which

show this sequence has the potential to form a G-

quadruplex structure. The results have shown that the

intensity of PCR product bands has been decreased by

increasing the TMPyP4 concentration especially 1 and 2

μM vs the control sample which is without TMPyP4. But

there were not any decreasing of PCR product intensity in

control negative sequence by increasing of TMPyP4 vs of

the control sample. Based on this study, there is a change

structure has been formed in this sequence after its

treatment by G-quadruplex stabilizer compare to the

control negative sequence. So this changing is probably

because of the G-quadruplex formation.

Keywords: G-quadruplex, Prostaglandin-Endoperoxidase-

2, Downregulation, PCR stop assay




10

Loading Jellyfish venom C-CfTXA-STxB

chimeric antigen in PLA-PEG-PLA

copolymers with a tri-block configuration

Mahdi Hosseinzadeh*, Hossein Honari

Biological Research Center, Faculty of Basic Sciences, Imam Hussein (pbh) University, Tehran, Iran


The venom of C. fleckeri box jellyfish contains a variety of

bioactive proteins that are cytolytic, cytotoxic,

inflammatory or lethal. Poly Lactic Acid is a

biodegradable and biocompatible polymer approved by the

FDA. Adding PEG to PLA lowers the zeta potential, yields

a higher uptake and does not absorb plasma proteins,

resulting in a higher shelf life in the bloodstream. The

cytolytic C.fleckeri toxin C-CfTX1-STxB chimeric

recombinant protein was produced by the research group

in the laboratory previously. The aim of this study was to

Nanoencapsulate above-mentioned chimeric product in

PLA-PEG-PLA tri-block purchased copolymer. To

investigate the increase in the potential of action after

nanoencapsulation by double emulsion solvent evaporation

techniques, acetone, due to the low boiling point, easy

removal and less toxicity was selected as a solvent. The

antigenicity potency of encapsulated C-CFTX1-STXB was

compared with the naked one.Based on the results, the

efficiency of encapsulating C-CfTX1-STxB in PLA-PEG-

PLA nanoparticles was approximately 71%. The

Appearance of the nanoparticles was studied under SEM

revealed an apparent uniformity in the dimensions below

100 nm, spherical and smooth surface with a Proper

density. DLS was employed to evaluate the particle size.

The data obtained from the study of the hydrodynamic

radius and the electrical potential of the particle surface

(zeta potential) also indicate that the nanoparticles are in a

stable range.The release of C-CfTX1-STxB reached 49.6%

after 30 days.Encapsulated and naked antigen and PBS

were injected into mice, then the antibody titer in their

serum was measured by ELISA and its quality was

measured by Western Blot. Considering that recombinant

C-CfTX1-STxB protein doesn't have cardiotoxicity and

neurotoxicity effects on mice, and the high homology of

selected segment and due to controlled and slow release in

copolymer this produced protein can be used as a

candidate for a vaccine against Jellyfish venom.

Keywords: Chironex fleckeri, C-CFTX1-STxB chimeric

antigen, PLA-PEG-PLA tri-block copolymers,

Nanoencapsulation

The effect of soluble form of VEGF8-109 on

vein endothelial cells

Shokoufe Rezaei1, Zahra Rezaei2, Valilollah Babaeipour3, Ahmad Farhad

Talebi1, Reza H.Sajedi2*

1Department of Microbial Biotechnology, Faculty of Biotechnology,

Semnan University, Semnan, Iran

2Department of Biochemistry, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran

3Department of Bioscience and Biotechnology, Malek Ashtar University

of Technology, Tehran, Iran * Corresponding author: [email protected]

Vascular endothelial growth factor (VEGF) is a primarily

endothelial cell-specific mitogen that plays a pivotal role

in both vasculogenesis and angiogenesis. As a key

regulator of neovascularization, it promotes embryonic

development, wound healing and female reproductive

functions. The function of VEGF is associated with

various medical disorders, including tumor growth and

metastasis, proliferative retinopathies and inflammatory

conditions such as rheumatoid arthritis and psoriasis.

VEGF elicits cellular responses through binding to the

receptor tyrosine kinases, VEGFR1 and VEGFR2. VEGF

is a dimeric molecule, each polypeptide chain contains

multiple intrachain disulfide bonds, forming a cysteine

knot motif. Soluble expression of receptor binding domain

(RBD) of VEGF-A (residues 8-109) in SHuffle strain was

optimized by recruiting of taguchi software.SHuffle strain

is genetically engineered E. coli that is capable of

oxidizing cysteines within proteins to form disulfide

bonds. The quantification of expressed VEGF-A8-109 was

performed by ELISA. The angiogenic potency of

expressed VEGF-A8-109 was investigated by the

proliferation assay on human umbilical vein endothelial

cells (HUVEC). The results revealed that the recombinant

protein can induce proliferation of HUVECand showed a

significant increase in proliferation rate compared to

control.

Keywords: VEGF, Angiogenesis, SHuffle strain, HUVEC

proliferation assay



11

Identifying ovarian cancer micro RNA bio-

Markers using a sequential wrapper method

Hanif Yaghoobi*, Esmaeil Babaei 1 Department of Animal Biology, Faculty of Natural science, University of Tabriz


A microRNA (miRNA) is a small non-coding RNA

molecule. The main task of microRNA is the post-

transcriptional regulation of gene expression. miRNAs can

act as either oncogenes or tumor suppressors by targeting

the expression of cancer-related genes. So, miRNAs can be

used as biomarkers for the diagnosis, prognosis, and

treatment of cancer. Microarray-based expression analysis

is a common approach for detecting candidate miRNAs

which are differentially expressed in normal and malignant

tissue samples. Biomarkers finding is equivalent to a

feature selection problem. The selection of a subset of

features increases the accuracy of classification and

reduces the cost of computation, clinical costs and the

possibility of over-fitting, which is likely to be increased

by increasing the number of miRNAs relative to the

number of samples. In this study, a sequential wrapper-

based approach was used to select biomarkers from

miRNAs involved in ovarian cancer. This method provides

the best prediction for the classification of cancerous and

normal samples by selecting a subset of miRNAs

sequentially and uses the LDA classifier. The proposed

method identified 8 out of 2565 miRNAs as biomarkers

that they can separate healthy and cancerous samples using

10-fold cross-validation and achieved an accuracy of

100%. These eight miRNAs include: hsa-miR-760, hsa-

miR-320b, hsa-miR-1290, hsa-miR-3197, hsa-miR-4258,

hsa-miR-6131, hsa-miR-6800-5p .We evaluated the

selected miRNAs by using their target genes and analyzed

Gene-miRNA pathway by using Cytoscape Software. The

analysis confirms the significant relationship between

selected biomarkers and ovarian cancer.

Keywords: Micro RNA, Biomarker, Feature Selection

Method, Sequential Wrapper Method

Investigation of bacteriophage infections of

potato soft-rot causal bacteria in potato farms

of Tabriz

Sohrab Pajnameh, Reza Khakvar*, Nemat Sokhandan Bashir 1 Department of Plant Protection, Faculty of Agriculture, University of Tabriz, Iran


Pectobacterium carotovorum subsp. the carotovorum

causal agent of bacterial soft-rot disease of potato is one

the most destructive disease in the northwest of Iran. Due

to the presence of this pathogen in the plant tissue and soil,

bacteria control is difficult. One of the least considered

controlling methods, is using bacteriophage for controlling

of the bacterial populations in the farm and warehouses.

Since there is no report for phage infections in the region

and high damages of this disease; this study was conducted

for primary identification of phage infections of causal

agent of bacteria soft-rot disease in potato farms of the

East Azerbaijan province. Bacteriophage isolation from

potato farms’ soil was performed using precipitation and

enrichment methods. In total, 30 soil samples were

collected and examined. Based on electron microscopic

imaging, three different phages were isolated. TEM

images were indicated that these phages have typical

morphology related to Myoviridae (Caudovirales).

Laboratory assessments showed that both three phages

have biocontrol capabilities for controlling bacterial

populations in potato tubers

Keywords: Pectobacterium carotovorum subsp.

carotovorum, Potato, Bacteriophage, Precipitation and

enrichment methods

12

Isolation of multiple drug-resistant genes on

exfoliative toxin B-encoding plasmid in

Staphylococcus aureus obtained from skin

infection samples

Elham Siasi*, Farzaneh Hossieni, Zibasadat Majid Seyed biglou

Department of Microbiology, Faculty of science, University, of North Tehran Branch, Islamic Azad. Tehran, Iran


The exfoliativetoxin is Staphylococcus aureus various

factorsthat can cause colonization and virulence.The aim

of this study was to investigate the presence of several

antibiotic-resistant genes on exfoliative B-encoding

plasmids in S. aureus-infected skin specimens. 100 wound

samples collected from Tehran Hospitals. Bacteria were

identified by standard biochemical and microbiological

tests. Antibiogram test was performed for Staphylococcus

aureus isolates. DNA was extracted

fromresistanceStaphylococcus aureus. Presence of

antibiotic-resistantetb, aac, and msrA genes was identified

by PCR. From 100 samples, 60 samples were

Staphylococcus aureus. Resistance to erythromycin,

gentamicin, tobramycin, ciprofloxacin and linezolid was 3

(5%), 1 (2%), 1 (2%), 1 (2%) and 0 (0%) respectively.

Frequency of msrA, aac and etb genes were 29 (48.3%),

45 (75%) and 37 (61.7%) respectively. Frequency for

simultaneously presence of the etb / aac, etb / msrA, msrA

/ aac and msrA / aac / etb gene were 28 (46.6%), 22

(36.6%), 22 (36.6%) and 18 (30%) strains. In this study,

the etb gene was indicated in most strains. As also,

investigation of virulence genes in Staphylococcus aureus

skin infection samples showed frequency of resistance

pathogen genes were increased.

Keywords: Staphylococcusaureus, Resistance genes,

Exfoliative toxin, Plasmid, Skin infection

The evaluation of antibiotic resistance pattern

and frequency of extended-spectrum beta-

lactamases among clinical isolates of

Pseudomonas aeruginosa isolated from

inpatients of Imam Khomeini hospital,

Kermanshah, Iran

Arman Rostamzad1*, Javad Najafeian2 1 Department of Biology, Faculty of Science, University of Ilam, Iran 2Department of Biology, Faculty of Sciences, Ilam University, Iran * Corresponding author: [email protected]

Pseudomonas aeruginosa is one of the most important

cases of nosocomial infections. The resistance of this

bacterium to different antibiotics especially to beta-lactams

and carbapenems is increasing. The aim of this study was

to determine of antibiotic resistance pattern and extended

spectrum beta-lactamases frequency in Pseudomonas

aeruginosa isolates. In this cross-sectional study, a total of

66 Pseudomonas aeruginosa isolates from Imam

Khomeini hospital in Kermanshah were collected. The

sensitivity of isolates using agar disk diffusion method

(Kirby- Bauer) to followed antibiotics: ceftazidime (30µg),

cefotaxime (30µg), imipenem (10µg), kanamycin (30µg),

co-trimoxazole (25µg), ciprofloxacin (5µg), ceftriaxone

(10µg), clindamycin (30µg), Cefoxitin (30µg), gentamycin

(10µg), was determined. The ESBL producing isolates

were determined using the combined disk and frequency of

PER and OXA beta-lactamases genes by using PCR was

evaluated. Among 86 isolates of Pseudomonas aeruginosa,

the highest rate of resistance was related to cefotaxime, co-

trimoxazole, kanamycin, ceftriaxone, clindamycin,

Cefoxitin, ceftriaxone was 100% and resistance to

gentamicin, imipenem, ciprofloxacin, and ceftazidime was

87.27 and 83.33 and 81.80 and 80.30% respectively. The

frequency of phenotypic ESBL producing of isolates was

91.91% and frequency of OXA was 87.5% and frequency

of PER gene was 78.12%. The results of this study showed

the high rate of resistance to different antibiotics among

Pseudomonas aeruginosa isolates so it’s necessary to

improved treatment methods.

Keywords: Extended-spectrum beta-lactamases,

Pseudomonas aeruginosa, Antibiotic resistance,

Kermanshah



13

An introduction to a plastic consumer

microbial isolate from soil samples of Iran

Mohadesseh Asadi, Neda Karami, Ehsan Azin*

Department of Biology, Faculty of Science, University of Tehran, Iran * Corresponding author:[email protected]

Impranil as one of the main polymer components in

plastics manufacturing is one of the degradation resistance

pollutants in the environment. Serious health problems

caused by these chemicals have highlighted developing the

efficient degradation methods. Therefore, this project was

aimed at isolation of impranil consuming microorganisms

as the sole source of carbon .Four soil samples were

collected from hydrocarbon contaminated regions and

transported to the laboratory under standard condition.

Isolation of capable microorganism in using impranil as

the carbon source was conducted in M9 minimal salt

medium with 1% impranil as the sole source of carbon.

Evaluation of clear zone formation on the M9 medium as

an indicator of impranil degradation capability confirmed

the growth of one fungal isolate in the presence of 1%

impranil. Microscopic and macroscopic characterization

of the selected isolated showed that the fungi belong to

Aspergillus genus. The fungi were cultured in M9 medium

with 2.5%, 5%, 7.5% and 10% NaCl in the presence of 1%

impranil in three replicates. At the end of incubation

period, the fungi showed to tolerate the salt up to 7.5%.

The further analysis should be performed to determine the

capacity of the fungal isolate in impranil removal.

Keywords: Impranil, M9 medium, Aspergillus sp., Plastic

degradator

Isolation of Shewanella spp. from sediments of

the Caspian Sea for electricity generation

from synthetic wastewater in microbial fuel

cells

Seyedeh Maryam Ekrami*, Mojtaba Mohseni

Department of Molecular and Cell Biology, University of Mazandaran, Babolsar, Iran


In recently with the increasing population, industrialization

and energy demand, the natural energy resources are being

exploited. A microbial fuel cell is a bio-electrochemical

system that converts chemical energy in organic

compounds to electrical energy through catalytic reactions

of microorganisms under anaerobic conditions. Isolation

and characterization of Shewanella spp.from sediments of

the Caspian Sea and evaluation of its ability to produce

electricity from synthetic wastewater were the aims of this

study.Samples collected from the sea sediments were

cultured in Kligler agar. After incubation at 30 °C, black

colonies were selected. After identification of isolates

based on morphology, physiology and molecular

characteristics, one was chosen and its ability to produce

electricity was evaluatedusing microbial fuel cells. The

isolate ME1 was inoculated in LB medium and incubated

at 30 ºC for 24 hours. Then the culture was transferred into

the anode chamber containing the synthetic wastewater.

Neutral red was used as an intermediate electron transport

and electrodes were made of graphite. Results showed that

the isolate was able to produce electricity with a maximum

open circuit voltage of 642 mV. In addition, a maximum

power density and a maximum current density was

evaluated 58.267 mW/m2 and 254.4 mA/m2, respectively.

The COD removal efficiency was 76%. The results of this

study demonstrated that the Shewanella ME1 isolated from

the sediments had high ability to produce electricity from

synthetic wastewater in microbial fuel cells.

Keywords: Microbial fuel cell, Shewanella, Synthetic

wastewater, Produce electricity


14

Investigation of Bacillus licheniformis OT9

isolated from the Tabriz refinery soil in

microbial enhanced oil recovery

Fatemeh Notghi Oskui1, Gholamreza Zarrini2*, Hassan Aghdasinia1,

Mohammad Ali Hoseinpour Feizi2, Farideh Zayermashhad Bonab2 1 Faculty of Petroleum and Chemical Engineering, University of Tabriz,

Iran 2 Laboratory of Microbiology, Department of Animal Sciences, Faculty of Natural Sciences, University of Tabriz, Iran

* Corresponding author: g [email protected]

Crude oil is the most important energy source in most of

the industrial countries. Microbial enhanced oil recovery

(MEOR) is a cost-effective method that uses

microorganisms or their metabolites to extract the residual

oil. The aim of this study is an investigation of effective

bacteria in MEOR. In this research, oil contaminated soil

and water samples were gathered from Tabriz refinery and

the effective bacteria were separated. Identification of

selected isolates was performed by sequencing of the

16SrRNA gene. MEOR efficiency of these bacteria was

studied using the syringe method. Investigations were done

in 1, 2, 4, 7 and 14 days of heating times with different

amounts of bacterial inoculation (0.1, 0.2, 0.5 and 1 mL) at

a temperature of . To measure the oil recovery factor,

3ml of 5% brine was injected and an adequate time was

given to the system until the oil was extracted. Finally,

recovered oil value was calculated from the results.

Among all microbial isolates, Bacillus licheniformis OT9

was characterized based on molecular and biochemical

methods and its performance in MEOR was investigated.

The highest recovery factor was attained in the heating

time case of 24 and 48 hours, due to accessibility of

nutrients for the bacterium and the highest values of

inoculation were 0.5 and 1 ml, since the number of

bacteria and produced metabolite value were higher and so

the effectiveness was better. The highest recovery factor

was 89.66 %, for to the injection value of 1 ml and heating

time of 48 hours.

Keywords: Microbial enhanced oil recovery, Bacillus

licheniformis, Soil, Oil contamination


15

Posters

16

Posters Contents

Inhibitory effects of phenolic compounds on human lipase

activity ............................................................................. 21

Isolation of immunodominant proteins of Naja Naja

(oxiana) snake venom ...................................................... 21

Plant anticancer peptides, a meta-analysis study ............. 22

Study of ct-DNA interaction with celecoxib (celberx) .... 22

Investigation of absorption wavelength changes in Griess

microassay for detection of nitric oxide .......................... 23

Association between blood Lead levels with Age ........... 23

The pH stability and the influence of salts on the activity

of a milk-clotting enzyme from ....................................... 24

F. johannis latex .............................................................. 24

Caseinolytic and milk-clotting activities of a new protease

from Ficus johannis and its enzymatic activity on the

different substrate ............................................................ 24

A spectroscopic and molecular docking approach to

investigate the interaction of thioridazine with ct-DNA .. 25

Effects of farnesiferol A on structure and activity of

bovine liver catalase: using experimental and simulation

methods ........................................................................... 25

Binding interaction of perphenazine with calf thymus

DNA: a spectroscopic and molecular docking study ....... 26

Oxytocin stands against 3-NP induced HD like disease in

male and female rats ........................................................ 26

Comparative studies on the interaction between glycerol

polyol with bovine trypsin: spectroscopic and theoretical

approaches ....................................................................... 27

A comparison of flavonoid and phenolic content of

different parts of Eryngium planum ................................ 27

Improved thermal stability of laccase immobilized on

carboxyl functionalized chitosan magnetic nanoparticles 28

Evaluation of cystatin-C by ELISA assay in rats with

diabetes mellitus supplemented by zinc oxide

nanoparticles ................................................................... 28

Evaluation of Zinc level in the serum of hypo and

hyperthyroidism patients in Urmia County ..................... 29

Immobilization of laccase enzyme on Iron Oxide

nanoparticle and determination of its activity ................. 29

Thiolation of Chitosan nanoparticle and immobilization of

Laccase enzyme............................................................... 30

Immobilization of α-amylase on nanoporous zeolite:

improved stability and reusability ................................... 30

Measurement of 17α-hydroxyprogesterone (17α-OHP) by

using solid-phase extraction and HPLC method ............. 31

The improvement of the protein profiling of Ailanthus

altissima pollen extract using high-length immobilized pH

gradient as the first dimension for 2-DE ......................... 31

Alpha-glucosidase inhibitory activity by hexane extract

from different aerial parts of plants, Haplophyllum

acutifolium DC. and Ferula haussknechtii Wolff ex Rech

......................................................................................... 32

Investigation of covalent attachments between

functionalized tungsten disulfide and anti- Hepatitis B

antibody ........................................................................... 32

Study of the beneficial effects of Tsukamurella

inchonensisin STZ induced type I diabetic rat intestine ... 33

Study of the protective effects of Gordonia bronchialisin

heart injury of diabetic rats .............................................. 33

Evaluation of the inhibitory effect of Trachyspermum

copticum fractions on AGEformation in the diabetic model

on in vitro ......................................................................... 34

Determination of the effect of cinnamaldehyde of

cinnamon on the urease activity ....................................... 34

Inhibition of urease activity by the main chemical

component of clove oil ..................................................... 35

Measuring ofcatalase activity in liver tissue of mice treated

with acetaminophen and Spirulina alga ........................... 35

Investigation of the effect of a sedative drug, Donepezil,

on the activity of peroxidase ............................................ 36

Tyrosinase inhibitory and antioxidant activity of

methanolic extract from various aerial organs of

Astragalus siliquosusBioss and Verbascum phoeniceum L.

......................................................................................... 36

Baicalein incorporated nanoliposome disaggregates alpha-

synuclein fibrils ................................................................ 37

The acetylcholinesterase inhibitory activity and

antioxidant properties of methanol extract of different

organs of Euphorbia macrocladaBioss and Glaucium

grandiflorumBioss and Huet ............................................ 37

Synthesis of diazo dyes from 2, 6-diamino-4-

chloropyrimidine compound and the biological evaluation

of their effects on tyrosinase activity ............................... 38

Design, synthesis and biological evaluation of 2,4,6-

triaminopyrimidine derivatives as tyrosinase inhibitors... 38

Isolation and characterization of the c-type lysozyme-

encoding gene from Acipenser persicus........................... 39

New derivatives of imine as inhibitors of

acetylcholinesterase (AChE): Synthesis, biological

evaluation, antioxidant activity and molecular docking ... 39

Adsorption and desorption studies of cadmium by cross-

linked chitosan/κ-carrageenan .......................................... 40

Study of cinnamaldehyde and eugenol binding to catalase

using molecular docking approach ................................... 40

Purification of a protease from an organic-solvent tolerant

alkalophilic Bacillus sp. ................................................... 41

Artificial neural network for monitoring the antioxidant

status of human plasma .................................................... 41

Design and construction of intra-chain disulfide urate

oxidase in Aspergillus flavus ............................................ 42

Comparison of antioxidant properties of two herbal

carotenoids with crab (Portunus pelagicus) hemolymph . 42

Extraction of saponins fromTribulus terrestris and

evaluation of its effects on human serum albumin (HSA)

structure by UV-visibleandFT-IR spectroscopies ............ 43

17

Investigation of benzene biological effect on hen egg white

lysozyme structure and function ...................................... 43

Study of eye lens alpha crystalline structural changes upon

interaction with methyl tert-butyl ether (MTBE) ............ 44

Investigation of the binding mechanism and inhibitory

effect of 2-hydroxy-1,4-naphthoquinone on catalase

activity and structure: multi-spectroscopic and

computational study ........................................................ 44

Inhibitory effect of Orange Yellow S on the structure and

the function of catalase: Spectroscopic methods combined

with theoretical studies .................................................... 45

Stabilizing of catechol 1,2 dioxygenase on the surface of

magnetic nanoparticles and investigating the enzyme

activity ............................................................................. 45

Investigation of activity the catechol 2, 3 dioxygenase

immobilized on the surface of silica nanoparticles ......... 46

Investigating the activity of cyclomaltodextrinase

stabilized on the surface of superparamagnetic iron oxide

nanoparticles with modified core-shell ........................... 46

Detection of H2O2 and glucose using peroxidase

mimicking activity of CuO/GFs aerogel ......................... 47

Effect of 3-beta-hydroxybutyrate on the formation of

human serum albumin amyloid fibrils ............................. 47

Investigation of the inhibitory effect of two new phenol-

ninhydrin derivatives on humansalivary α-amylase enzyme

......................................................................................... 48

Keywords: Diabetes, Human salivary alpha-amylase,

Ninhydrin pyrogallol derivatives ..................................... 48

Lipase immobilization on aluminum-based periodic

mesoporous organosilica (PMO) support as a biocatalyst

for biodiesel production .................................................. 48

The study of protective effects of Propolis against X

radiation on MCF-7 cell line ........................................... 49

Study of the interaction between indinavir and complex

(DNA-H1) by multispectroscopic techniques ................. 49

Study of the interaction between Nelfinavir and complex

(DNA-H1) bymultispectroscopic techniques .................. 50

Changes of serum level of prolactin following the X-ray

radiation........................................................................... 50

Studies on the interaction of the drug Indinavir with calf

thymus DNA by resonance light scattering and circular

dichroism spectroscopy ................................................... 51

Studies on the interaction of the drug Nelfinavir with calf

thymus DNA by resonance light scattering and circular

dichroism spectroscopy ................................................... 51

The effect of different coating surface on Silver

nanoparticles interaction with human serum album (HSA)

......................................................................................... 52

The structural changes of hormone Human Chorionic

Gonadotropin (hCG) in Ultrasound exposure ................. 52

Effect of ultraviolet radiation on total phenol in Thymus

vulgaris L. ....................................................................... 53

Magnetic nanocomplex design for transferring cisplatin 53

Purposeful transfer of polyethyleneimine polymer using

magnetic nanoparticles and static magnetic fields to

normal and cancerous cells.............................................. 54

The effect of electromagnetic pulses on the glutamate-

aspartate transporter (GLAST) and glutamine synthase

(GS) in the hippocampus of male rats .............................. 54

The magnetic Co1-xZnxFe2O4 nanostructure interaction

with DNA molecule study by multiple spectroscopies .... 55

Using a genetic algorithm to find promoter and motif ..... 55

The effects of L-dopa on hypothalamic NPY gene

expressions in PCOS model rats ...................................... 56

Ancient DNA extraction, identification, molecular cloning

and enzymatic enhancement ............................................ 56

In silico study of the effect of two N-terminal SNPs in E-

cadherin protein on its structure, function, and stability .. 57

Identification of genes involved in herbal docetaxel-

resistant prostate cancer ................................................... 57

Identification of genes involved in enzalutamide-resistant

prostate cancer ................................................................. 58

Effect of silica-chitosan nanocomposite encapsulated

epigallocatechin gallate on SKOV3 ................................. 58

Study of the simultaneous coating of electrospun

nanofibers with bioactive molecules for stem cell

osteogenesis in vitro ......................................................... 59

Clinicopathologic features in HER2-positive breast cancer

women in Kermanshah ..................................................... 59

The study of the effects of Cucurbitacin E from Ecballium

elaterium (L.) A. Rich on LC-3 gene expression in human

gastric cancer cell line AGS ............................................. 60

Hepatocyte growth factor (HGF) serum concentration and

promoter polymorphism in risk prediction of autism

spectrum disorder ............................................................. 60

Study of MACC1 gene expression in blood samples of

patients with colorectal cancer in west and northwest of

Iran ................................................................................... 61

Evaluation of APC gene mutation in ctDNA of patient

with colorectal cancer in northwest of Iranwest and ........ 61

Y-chromosome identification in circulation cell-free fetal

DNA by PCR ................................................................... 62

In Silico studies of Congenital Adrenal Hyperplasia

(CAH), caused by CYP21A2 gene mutation.................... 62

In silico modeling and characterization of L-asparaginase

from bacteria, plants and fungal sources, using

computational tools and online servers ............................ 63

Bioinformatics comparison ofSOX9, FOXP2,

DUF1220,APOC1,SIGLEC13,CLLU1, AQP7, PDYN and

HAR1genes in human and chimpanzee ........................... 63

Contribution of the bHLH-transcription factor gene family

to male infertility: a comprehensive gene prioritization

analysis ............................................................................. 64

In silico analysis of immune system stimulation by

asparaginase enzymes produced by bacterial endophytes 64

In Silico design of multimeric antigen as a highly

immunogenic peptide vaccine against Bordetella pertussis

......................................................................................... 65

Comparative evaluation of silibinin effect on apoptosis in

human breast cancer MCF-7 cell line in vitro and in vivo

......................................................................................... 65

18

Genetic evaluation of cold atmospheric plasma, Jasmonic

acid and Spermine treatments on Catharanthus roseus (L.)

seeds by TRAP marker .................................................... 66

A deep insight into the existed introns in the 18S rDNA

gene of Dunaliella species .............................................. 66

Presenting a novel method for classification of Dunaliella

species: a new approach, which uses 18S ribosomal DNA,

ITS and rbcL molecular markers ..................................... 67

The effect of cold plasma, methyl jasmonate and

putrescine on genetic variation of Catharanthus roseus

(L.) ................................................................................... 67

Investigation of repeatability of presence and effect of the

rs3918242 in patients with autism spectrum disorder ..... 68

The study of resistin gene expression changes in adipose

and stomach tissues of male rats subjected to chronic

immobilization stress ....................................................... 68

The Effect of hydroalcoholic extract of spinach on leptin

gene expression changes in adipose and muscle tissues of

male rats subjected to chronic immobilization stress ...... 69

CXCL8 (IL-8) genetic variation (rs4073) in the patients

with ASD in Guilan population ....................................... 69

Insulin-like growth factor-1 circulating concentrations and

Promoter Polymorphism in Risk Prediction of children

with autism spectrum disorders ....................................... 70

Bioinformaticsanalysisoflong- non-coding RNA

(LncRNA) in azoospermia .............................................. 70

Prediction of the effect of hsa-miR-3680-3p and hsa-miR-

671-5p on azoospermia ................................................... 71

Structure and distribution of WD40 genes in sunflower

(Helianthus annuus L.) chromosomes ............................. 71

Assessment of relationships of phylogenetic WD40 protein

in sunflower (Helianthus annuus L.) by a bioinformatics

approach .......................................................................... 72

Bioinformatics investigation of the structure and function

of mnemiopsin2 following proline 181 substitutions using

a site-directed mutagenesis .............................................. 72

Effect of Valine 172 residue alteration using a site-directed

mutagenesis on the structure and function of

mnemiopsin2: a bioinformatics study ............................. 73

Comparison of TCEB3 gene expression between breast

cancer tissues and the adjacent non-tumor tissues ........... 73

Microarray s gene expression analysis in breast cancer

using system biology approaches .................................... 74

Expression of miR-7, miR-409 and miR-93 in patients

with colorectal cancer who referred to Tehran hospitals by

Real Time PCR ............................................................... 74

Association between SGSM3 gene (rs 17001868)

polymorphism and breast cancer in East Azarbaijan

population ........................................................................ 75

The study of IDOL gene expression changes in adipose

tissue of male rats subjected to chronic immobilization

stress ................................................................................ 75

The effect of simulated microgravity on RKIP tumor

suppressor gene expression in MCF-7 breast cancer cell

line ................................................................................... 76

Evaluation of nerve growth factor (NGF) methylation

status in patients with schizophrenia ............................... 76

Determination of 3D structure and properties of

cytochrome P450 enzymes in entomopathogenic fungus

Beauveria bassiana .......................................................... 77

Evaluation of Hsa-miR-940 expression in tumoral and

marginal tissues of the patients with breast cancer .......... 77

Expression analysis of Long non-coding RNA SNHG17 in

breast cancer ..................................................................... 78

Evaluation of methylation and expression of miR-96 in

tumor tissue versus margin in patients with breast cancer78

Evaluation of methylation and expression of miR-196b in

tumor tissue versus margin patients with breast cancer ... 79

Overexpression of α-Synuclein inSHSY5Y cell to generate

a model for Parkinson’s disease ....................................... 79

Sequencing of the acetolactate synthase gene in the milk

thistle, Silybum marianum (L.) Gaertn ............................. 80

DSCAM-AS1 lncRNA upregulates in ductal breast cancer

tumoral tissues ................................................................. 80

Identification of the key genes/proteins in hepatitis B virus

and hepatocellular carcinoma via functional clusters in a

protein-protein interaction network .................................. 81

Evaluating and designing contraceptive vaccine and

recombinant fusion protein based on IZUMO, SPRASA

and PH-20 epitopes .......................................................... 81

DNA methylation analysis of the pro-inflammatory IL6

gene in Type 2 Diabetespatients ...................................... 82

DNA methylation analysis of pro-inflammatory genes in

patients affected with type 2 diabetes .............................. 82

Isolation and identification of the c-type lysozyme-

encoding gene from Salmo trutta caspius ........................ 83

Resveratrol and breast cancer: the survival of cancer cells

and expression of caspase gene 3 ..................................... 83

Improvement the effect of green synthesized nano-oxali

palladium in comparison with oxali palladiumagainst

human colon cancer cell line HCT116 ............................. 84

Design of diazo dyes based on 2, 6-diamino-4-

chloropyrimidine compound and the analysis of their

interaction with tyrosinase using molecular docking

method ............................................................................. 84

Investigation of the gene expression profiling of

photoreceptors in separated reproductive and somatic cells

in multicellular green algae Volvox carteri at low intensity

of UV-B radiation ............................................................ 85

A comparative transcriptome analysis of two cell-types of

colonial green alga Volvox carteri ................................... 85

Molecular docking of 2,4,6-triaminopyrimidine derivatives

as tyrosinase inhibitors ..................................................... 86

Association between ADSL gene (rs 3788577)

polymorphism and breast cancer in East Azarbaijan

population ........................................................................ 86

Investigation ofinteraction of a novel synthetic acridine-

derived inhibitor with acetylcholinesterase enzyme by

molecular docking ............................................................ 87

Investigating the conformity of the second law of Chargaff

on variable-length homopolymer fragments in the human

genome ............................................................................. 87

Study of MFN2gene expression in tissue samples of

patients with breast cancer ............................................... 88

19

Association of 87851G>A, LMTK2 nucleotide transition

with benign prostatic hyperplasia: a case-control study in

Mazandaran population ................................................... 88

Jellyfish venom C-CFTX1-STxB chimeric antigen

subcloning, expression and its antigenicity assay in

laboratory mouse ............................................................. 89

Investigation of the expression of two long non-coding

RNAs (KCNQ1OT1 and MALAT1) in peripheral blood of

patients with acute myeloid leukemia ............................. 89

Overexpression of VOPP1 and PIK3C2B genes in chronic

lymphoblastic leukemia ................................................... 90

Expression of recombinant EGFP viasurface display of ice

nucleation protein and TEV protease cleavage site ......... 90

Comparison of two conventional molecular methods in

detecting jak2v617f mutations in patients with

myeloproliferative neoplasms ......................................... 91

Study of the differentiation of rat omentum stem cells to

nerve cells using brain tissue extract of rat ..................... 91

Study of gene Polymorphisms correlated with allergic

rhiniris disease in the northwest of Iran .......................... 92

Decreasing of viability in H2O2treated of ITPA down-

regulated human umbilical vein endothelial cells ........... 92

Bioinformatically study of D1 and D2 proteins in two

species of Chlorella ......................................................... 93

Application of bioinformatics in the design of anti-VEGF

peptide ............................................................................. 93

Bioinformatics predictionof long-non coding RNAs as

expression regulatory candidates of genes involving in

myelination ...................................................................... 94

Designing, synthesizing and cloning of equine follicle

stimulating hormone in prokaryotic host ......................... 94

Investigating the anti-Alzheimer's properties of Desf:

studying the inhibitory effects of the Desf extract on the

production of amyloid nanobiofibrils and measuring its

antioxidant activity .......................................................... 95

Evaluation of Limonene synthase gene expression in

Peppermint plants under abiotic stresses ......................... 95

Study of the expression of isopiperitenone reductase gene

in peppermint (Mentha piperita) ..................................... 96

Investigation of changes in the expression of RNA-

helicase (MOV10L1) gene in transgenic embryonic stem

cells of rat exposed to retinoic acid ................................. 96

Investigation of MOV10L1 gene expression in embryonic

stem cells of OCT4-GFP in rats affected by retinoic acid

and human follicular fluid ............................................... 97

Post-training administration of morphine alters expression

of mir33 in rat .................................................................. 97

A study on bioinformatical properties of gene 5a among

different strains of infectious bronchitis virus ................. 98

Bioinformatic analysis of gene 5b from different strains of

infectious bronchitis virus ............................................... 98

Spermatogenic and phylogenetic characterizations of

isolated fasciola sp. from natural host (cattle) in north west

of iran .............................................................................. 99

Investigation of microbial agent damaging to historical and

cultural monuments ......................................................... 99

Assessment of new antibiotics application for controlling

of bacterial canker of stone fruits in laboratory conditions

....................................................................................... 100

The effect of the common pesticides in the Khoy city on

bacterial canker of stone fruits ....................................... 100

Frequency of TEM beta-lactamase resistance gene in

patients with urinary tract infections in Bonab County .. 101

Effect of Bifidobacterium strains isolated from baby feces

on Acinetobacter biofilm ............................................... 101

Study of the antibiotic resistance pattern and frequency of

streptomycin, trimethoprim, gentamycin, sulfonamides and

chloramphenicol resistance genes in Escherichia coli

isolated from urinary tract infections of women in Tabriz

city ................................................................................. 102

Oral administration of Lactobacillus rhamnosus has

improving effects on burn wound healing in rats ........... 102

Identification and isolation of the Iranian native bacteria

producing cellulase enzyme ........................................... 103

Antibiotic resistance pattern and molecular characteristics

of Staphylococcus aureus isolated from the nasal carriage

of health care workers in two private hospitals in Tabriz,

Iran ................................................................................. 103

Antibiotic resistance pattern of Escherichia coli isolated

from patients with urinary tract infection in Sarab, Iran 104

Evaluation of the frequency of class I integron gene and

antibiotic resistance pattern in Escherichia coli isolated

from patients with urinary tract infections in Imam

Khomeini Hospital in Sarab, Iran ................................... 104

The efficiency of the cold argon-oxygen plasma for

controlling the fungal of documents in cultural heritage 105

Molecular diagnosis of class 1 integrons in Acinetobacter

baumannii strains isolated from patients admitted in

hospitals of Sari .............................................................. 105

A proteomics approach to identify metacyclogenesis

regulated proteins in Iranian clinical isolates of

Leishmania tropica ........................................................ 106

Determination of antibiotic resistance pattern in

Aeromonas hydrophila isolated from Reared

Oncorhynchus mykiss in Marand, Iran ........................... 106

Evaluation of the effects of silver nanoparticles and

alcoholic extract of Achillea wilhelmsii on pathogenic

bacteria Staphylococcus aureus, Bacillus cereus,

Escherichia coli and Pseudomonasaeruginosa .............. 107

Assessment of the antimicrobial effect of Cistanche sp. on

the planktonic forms and biofilm structures of pathogen

bacteria ........................................................................... 107

Antimicrobial effect of 5 Nepeta native species of Kerman

Province ......................................................................... 108

Studying antimicrobial effect of fermented probiotic milk

using Lactobacillus and Bifidobacteria strains .............. 108

Effect of salt on saliva antibacterial property on

Staphylococcus mutants and Staphylococcus aureus

bacteria ........................................................................... 109

Aqueous and ethanolic extracts of Allium hirtifolium and

Allium sativumon growth inhibition of Candida tropicalis

in a systemic candidiasis mouse model .......................... 109

20

Determination of antibiotic resistance pattern and

molecular diagnosis of β -lactamase genes (blaSHV,

blaTEM, blaCTX-M) in Klebsiella pneumonia isolates

from in-patient of Ilam hospitals ................................... 110

The evaluation of beta-lactamases genes in E.coli species

isolated from hospitals sewages in Ilam city ................. 110

Screening of L-asparaginase producing strains isolated

from honey in different regions of Iran ......................... 111

Isolation of fast growth and acid resistance probiotics from

Golpayegan yogurt ........................................................ 111

Designing a novel signal peptide for secretion of

recombinant Human activin A protein through the twin-

arginine translocation (Tat) pathway in E. coli ............. 112

Investigation of antimicrobial properties of n-hexane

extract of Onosmastraussii ............................................ 112

Effect of synergist Aloe Vera extract and supernatant

Lactobacillus fermentum from a local cheese (Kozeh) on

Klebsiella bacteria ......................................................... 113

The effect of probiotic Lactobacillus on the control of

weight and on serum leptin and adiponectin status in

streptozotocin-induced diabetic rats .............................. 113

Anticoccidial effect of metabolites of native Streptomyces

on prevalent eimeria of Iran .......................................... 114

Histopathological evaluation of liver and spleen after

immunized mice with recombinant PilQ, b-flagellin

vaccine ........................................................................... 114

Immuno-magnetic diagnosisof Brucella abortus based on

of iron and graphene nanoparticles ................................ 115

Isolation and identification of nitrogen-fixing rhizospheric

bacteria from Narcissus flower ...................................... 115

Evaluation of antibacterial activity of methanolic extract of

the polypore fungus Phellinus sp.isolated from

Mazandaran forests ........................................................ 116

Antibacterial activity of Stachys persica from Labiatae 116

Isolation and identification of an Actinomycete isolate

capable of producing anti-MRSA compound from soil

samples and optimization of production in liquid culture

....................................................................................... 117

Prevalence of coagulase positive Staphylococcus

pseudintermedius in some domestic animals ................. 117

Study and evaluation of antibacterial properties of clove

essential oil..................................................................... 118

21

Inhibitory effects of phenolic compounds on

human lipase activity

Ghazal Khooshehchin1, Farideh Razi2, Parichehreh Yaghmaei1, Azadeh

Ebrahim Habibi3 1

Department of Biology, Science and Research Branch, Islamic Azad

University, Tehran, Iran 2 Diabetes Research Center, Endocrinology and Metabolism Clinical Sciences

Institute, Tehran University of Medical Sciences, Tehran, Iran 3 Biosensor Research Center, Endocrinology and Metabolism Molecular -

Cellular Sciences Institute, Tehran University of Medical Sciences, Tehran,

Iran

* Corresponding author:[email protected]

Lipase plays an important role in lipid digestion, therefore by using of lipase

inhibitors, fat absorption can be decreased and potentially result into weight

loss. Phenolic compounds have been reported as lipase inhibitors. In this

study, the effects of 14 phenolic compounds on lipase activity were examined.

Our results showed that 4,4′-isopropylidenediphenol, bithionol,

hexachlorophene, and diethylstilbestrol can be considered as a lipase inhibitor, while diethylstilbestrol has the highest inhibitory effect on lipase. Human

lipase (LPS) is a single-chain glycoprotein with 449 amino acids and a

molecular weight of 52 KD that hydrolyze glycerol esters of triacylglycerols

LPS can be inhibited by various natural and chemical compounds In this

research, the inhibitory effect of phenolic compounds have been investigated

on LPS. In this in vitro study we analyzed the effect of these 14 compounds on

lipase activity: 2,6-Diisopropylphenol, 3,5-Diiodosalicylic acid, Carvacrol, 4-

Chloro-2-isopropyl-5-methylphenol Cumylphenol, Bithionol, Bis(4-hydroxyphenyl) methane, 4,4′-Cyclohexylidenebisphenol, Diethylstilbestrol,

Diflunisal, 4,4′-Isopropylidenediphenol, 4,4′-Isopropylidenebis(2,6-

dimethylphenol), Hexachlorophene and Tolmetin. All these compound

purchased from Sigma (ST Louis USA). Dimethylsulfoxide (DMSO)

(Merck/Darmstadt/Germany) was used as a solvent for the preparation of

different concentrations (65, 325, 650, 3250 mMOL) of above-mentioned

compounds. Lipase activity was measured in normal (Trulab N) and

pathologic (Trulab P) control sera using the commercial kit (Pars azmun, Tehran, Iran) and automated chemistry analyzer ( Hitachi 902 Made in Japan )

before and after addition of each prepared solutions to kit’s reagent. The pdb

format compounds were downloaded from zinc.docking.org and docked into

LPS structure (1LPB pdb) with the use of www.swissdock.ch and the result

wasanalyzed by UCSF chimera 1.11rc, LIGPLOT⁺ , and PYMOL. Our results

showed that double- ring phenolic compounds have a stronger capability to

inhibit LPS than compounds with one ring which may be due to the increased number of phenolic hydroxyl groups. In double- ring phenolic compounds, the

presence of chloride or methane groups (and also their number) can also

influence the inhibitory effects. The overall results have been reported in

table1. Few compounds with one ring such as 2,6-diisopropylphenol, 3,5-

diiodosalicylic acid and 4-chloro-2-isopropyl-5-methylphenol had an activator

effect on LPS. We found Lowest Delta G for the interactionof bithionol,

hexachlorophene, 4,4′-isopropylidenediphenol and diethylstilbestrol with LPS.

The maximum inhibitory effect was obtained by diethylstilbestrol. The highest number of hydrophobic and hydrogen bonds with LPS were found for

bithionol and 4,4′-cyclohexylidenebisphenol respectively. For the best

compounds hexachlorophene, 4,4′-isopropylidenediphenol and

diethylstilbestrol, half maximal inhibitory concentration (IC50) was separately

estimated for Trulab N and Trulab P using linear regression and the results

were as follows: Diethylstilbestrol: 3.87 and 3.91 mMol, Hexachlorophene:

4.5 and 7.3 mMol,4,4′-Isopropylidenediphenol: 4.1 and 8.5 mMol. Using C11-

alkyl-3- phosphonate as a well-known LPS inhibitor (5) for comparative means, we evaluated the potential amino acids involved in the interaction

between LPS and diethylstilbestrol, hexachlorophene, bithionol, and 4,4′-

isopropylidenediphenol. The results demonstrated that His263- Phe215- Phe77

and Gly76 are common amino acids in the reactiontoabove-mentioned

compounds and LPS. The results of the present study showed that

bithionol,hexachlorophene, 4,4′-isopropylidenediphenol,and diethylstilbestrol

have an inhibitory effect on LPS. Among them, diethylstilbestrol is the most powerful inhibitor with 3 hydrogen bonds, 15 hydrophobic bonds, delta G (-

8.2) and 41% of LPS inhibition. The second one is 4,4′-

isopropylidenediphenol with 4 hydrogen bonds, 13 hydrophobic bonds, delta

G (-7.5) and 33% of LPS inhibition. In third place, we have hexachlorophene

with 16 hydrophobic bonds, delta G (-8.3) and 32% of LPS inhibition and the

last one is bithionol with 21 hydrophobic bonds, delta G (-8.0) and 23% of

LPS inhibition.

Keyword: Lipase, Inhibitor, Phenolic compound

Isolation of immunodominant proteins of

Naja Naja (oxiana) snake venom

Mahboobeh Talebi Mehrdar

Department of science, Faculty of biochemistry, University of Payame noor, Tehran, Iran


Snake venom is a complex mixture of proteins, peptides,

enzymes, carbohydrate, and mineral. Snake venom

contains a variety of chemicals including pharmacological

and toxicological properties. The innate immune system is

the first line of defense against toxin and microbe. The aim

of the present study is to investigate and isolate

immunodominant proteins of Naja oxiana snake venom.

Identification was performed by SDS-PAGE 15% and

western blot analysis. Subsequently, four sharp protein

bonds 14, 22, 32, 64 kDa, were appeared in nitrocellulose

paper. The next step identified proteins were isolated

directly by Electro-elution from preparative gel

electrophoresis. To the best our knowledge, these proteins

may be a candidate for specific antivenom or antiserum

against Naja oxiana.

Keywords: Naja Naja, Snake venom, Immunodominant

protein, Electro-elution



22

Plant anticancer peptides, a meta-analysis

study

Leila Zarandi-Miandoab*, Elaheh Zadeh Hosseingholi Department of Biology, Faculty of Basic Science, Azarbaijan Shahid

Madani University, Tabriz, Iran


Despite significant advances in cancer treatment, the interest in

designing new drugs has increased due to the increased resistance

of cancer cells to current anticancer drugs. Recent studies suggest

that some of the antimicrobial peptides (Anti-Microbial peptides)

have a wide range of cytotoxic activity against cancer cells.

These anticancer peptides alone or in combination with other

conventional drugs can be considered as a promising strategy in

cancer treatment.It seems that the use of herbal peptides with

high and stable anticancer activity in the serum, due to easy oral

administration, is the appropriate option in clinical cases. The

aim of this study was to investigate the detection of herbal anti-

cancer peptides and also to find the most common features

among them. In this regard, a list and information of the

antimicrobial peptides was exploitation from The Antimicrobial

Peptide Database. Statistical analyzes were performed using

RStudio software. The results indicated that the total number of

herbal peptides with proven anti-cancer effects were 55 cases.

Taxonomically, most of the peptides belong to Malpighiales

order. The order of Malpighiales is one of the largest flowering

plants orders, accounting about 7.8% of the total dicots from

Salix to Violets and Cacao. The Gentianales,

Fabales,andSantalales orders are in the next ranks. Also, the

Violaceae (violets) family has the largest share in the anti-cancer

peptides. It is noteworthy that all parts of violets (roots, stems,

leaves, flowers, and seeds) have anticancer effects. The

Rubiaceae, Fabaceae, andSantalaceae families were ranked next.

Varieties of peas and beans, chassalia, some types of mistletoe,

wild coffee, green coconut water, avocado fruits and hedyotis

(Chinese herb) could inhibit all types of tumors and cancers. The

length of about 44% of peptides was in the range of 25 to 30

amino acids. Histidine and methionine had the lowest abundance

among peptide amino acids. Cysteine, serine, and glycine were

high abundant amino acids. About 85.5% of the total peptides

contained 20 to 40% of the hydrophobic amino acids. 91% of

peptides had less than 10 acidic amino acids and 71% of peptides

had less than 10 basic amino acids. Approximately 96% of the

peptides had more than 40% neutral amino acids. A pure charge

of 76% peptides was between -2 and 2. 64% of peptides had a

Bowman index of less than 1. The low index indicates high

hydrophobicity of these peptides and increases their chances for

interacting with other proteins. Also, the most known important

three-dimensional structure for plant anti-cancer peptides was the

presence of 3 disulfide bridges. A three-dimensional structure of

a number of peptides had not yet been identified; however, there

were peptides with a combine Helix and Beta structure. 51% of

the peptides were only anti-cancer, but 49%, had anti-viral, anti-

microbial, anti-fungal, and anti-mammalian cells effects in

addition to the anti-cancer effects. The most of peptides had been

discovered in 2011 and 2012.

Conclusion: Manufacturers and drug designers can synthesize or

discover new effective drugs with low side effects using the

properties of anti-cancer plant peptides and the commonality of

most anti-cancer peptides extracted from advanced statistical

analyzes.

Keywords: Anticancer Peptides, Meta-Analysis study, RStudio

Software

Study of ct-DNA interaction with celecoxib

(celberx)

Nasrin Kazempour*

Department of Biology, Faculty of Science, Urmia University, Iran * Corresponding author: [email protected]

In this paper, the interaction of celcioxide with

cytomegalovirus DNA (DNA) using ultraviolet-visible

spectroscopy, fluorescence spectroscopy, electrophoresis,

infrared spectroscopy, molecular docking, in different

concentrations of ct-DNA and celecoxib, with the goal of

designing more effective countermeasures Cancer has been

studied with low side effects. The visible UV-absorbance

spectrum of celecoxib showed an effect of

hyperchromicity in the presence of DNA, and a constant

binding of cecoxib with a DNA of 2/7 ×104 was estimated.

The results show that celecoxib bonded to the DNA

through the groove (connection of the groove type).All of

the above experimental techniques confirmed the results of

visible-ultraviolet absorption spectroscopy in order to

detect the interaction of cell coccyb with the type of

groove (large or small) of Docking studies. The results of

this study, in addition to confirming the experimental

results, gave the type of interaction The action was

determined through a small groove with high affinity with

guanine cytosine.

Keywords: Interaction, Celecoxib, DNA of calf thymus,

spectroscopy, molecular docking


23

Investigation of absorption wavelength

changes in Griess microassay for detection of

nitric oxide

Soheila Shir Mohammadi1, Hossein Nahrevanian2*, Nematollah Razmi1 1 Department of Biochemistry, Islamic Azad University of Shiraz, Shiraz, Iran 2 Department of Parasitology, Pasteur Institute of Iran, Tehran, Iran


Griess colorimetric assay is based on azo dyes, which has

changed a lot today. This simple and inexpensive method

is used indirectly to detect nitric oxide (NO) in biological

and non-biological samples by means of RNI (Reactive

Nitrogen Intermediates). In this study, we evaluate some

wavelengths to improve this colorimetric assay using the

Griess microassay (GMA). Nowadays, the increasing use

of this assay in laboratories and research centers and even

kits have encouraged the researchers to study the physical

factor of optimized wavelength based on the Griess assay.

Although different wavelengths have been reported based

on this essay, it is still ambiguous to find out which

wavelength brings us closer to the best results in

measuring RNI. First, the Griess solution was prepared,

and then the sodium nitrite solution, prepared at specified

concentrations was added to draw the standard graph of

linear regression. Finally, wavelengths 480, 490, 500, 510,

520, 530, 540 were read and evaluated using a microplate

reader. Linear regression analysis at 480-510 wavelengths

had an increasing trend so that we observed a reduction in

linear regression as the wavelength changed from 510 to

540 nanometer (nm). By studying different wavelengths in

the GMA for detecting of NO to optimize it, the best linear

regression observed at 510 nm wavelength, which showed

the highest absorption in the standard curve, leading for

best detection of NO by GMA.

Keywords: Colorimetry, Griess, NO, Nitric oxide, Nitrite,

RNI, Regression

Association between blood Lead levels with

Age

Hanieh Babaei1, Maryam Sadat Daneshpour2*, Maryam Ghobeh1 1 Department of Biology, Faculty of Science and Research, Islamic Azad University, Tehran, Iran 2 Department of Biochemistry, Shahid Beheshti University of medical

sciences, Tehran, Iran * Corresponding author: [email protected]

Lead has many applications in the industry but does not

play a specific physiological role in the body. According to

performed studies, lead has an undesirable effect on the

nervous, gastrointestinal, respiratory, and endocrine

systems. People who are highly exposed to this element,

due to their occupation or living place, are affected by its

harmful effects. The aim of this study was to evaluate the

correlation between blood lead level and age. This cross-

sectional study was carried out on patients with high

exposure level lead in Tehran. After filling out the consent

form and questionnaire, the blood samples were collected

from them and their levels of blood leadwere evaluated in

a laboratory. Normal distribution of data was assessed by

Kolmogorov Smirnov test by Med Calc 14.8.1. There was

a weak link between blood lead levels and age (r = 0.25).

In his study, we observe the association between blood

lead level and age.

Keywords: Blood lead level, Lead, Age



24

The pH stability and the influence of salts on

the activity of a milk-clotting enzyme from

F. johannis latex

Moslem Afsharnezhad, S. Shirin Shahangian*, Reyhaneh Sariri

Department of Biology, Faculty of Science, University of Guilan, Rasht, Iran


Numerous studies have been carried out to replace calf

rennet with other milk-clotting proteases because of

limited supply and the high price of calf rennet. The latex

of Ficus johannis (Moraceae), rich in milk-clotting

proteases, have been traditionally used as a plant coagulant

for cheese-making. No systematic study on the

characterization of F. johannis milk-clotting protease has

been conducted so far. The purpose of this study was to

investigate pH stability and effect of salts on the activity of

the purified protease. The enzyme was extracted from the

latex of F.johannis and purified viacation exchange

chromatography.The proteolytic and milk-clotting activity

of the protease was evaluated using casein and skim milk

as a substrate, respectively.The pH stability determined by

examining the residual activity after incubating the

protease in different buffers with pH ranging from 3.0 to

11.0. The effect of different concentrations (0–3 mol/L) of

NaCl and CaCl2 ions on the enzyme activity was

determined.The molecular mass of the purified protease

was estimated to be 25 kDa by SDS–PAGE. The results

showed that the protease was almost completely active in

the presence of high salt concentrations.The protease is

stable under a broad range of pH between (4.5-9.5),

maintaining almost its complete activity. F.johannis

protease was efficiently active under different salts

concentration and in a wide range of pH. The purified

protease can be suggested as a suitable alternative to

commercial calf rennet in the dairy industry.

Keywords: Ficus johannis, Milk-clotting, Enzyme

activity, pH stability

Caseinolytic and milk-clotting activities of a

new protease from Ficus johannis and its

enzymatic activity on the different substrate

Moslem Afsharnezhad, S. Shirin Shahangian*, Reyhaneh Sariri

Department of Biology, Faculty of Science, University of Guilan, Rasht, Iran


Coagulation of milk is the main step for producing cheese,

and coagulating enzymes have been utilized for centuries

in cheese making. Chymosin (EC 3.4.23.4), as the main

enzymatic compound in calf rennet, has been widely used

as a coagulating agent in cheese making. Regarding the

high price of calf rennet as well as ethical considerations

of its, a great deal of effort has been made to find

appropriate milk coagulant substitutes produced either in

plants or microorganisms. Here, we evaluated the

caseinolytic and milk-clotting activitiesof a protease from

Ficus johannis. The protease with milk-clotting activity

was purified and the caseinolytic and milk-clotting

activities were assessed using casein and skim milk as a

substrate, respectively. The effects of protease inhibitors

on milk-clotting activity were also reported. Additionally,

the enzymatic activity of the purified protease on the other

different substrates was examined. The protease was

purified 9-fold with 44% activity recovery. The enzyme

was strongly inhibited by iodoacetamide, a typical cysteine

protease inhibitor, indicating that it belongs to the cysteine

protease family. The highest caseinolytic activity was

detected at pH 6.5 and 60°C temperature. The purified

protease exhibited considerable activity towards κ-casein

in comparison to α-casein and β-casein. According to the

present study, a novel milk-clotting cysteine protease from

F. johannis was characterized. The high degree of

hydrolysis on casein, in particular on κ-casein, high milk-

clotting activity and high clotting activity/proteolytic

activity ratio of the isolated enzyme could be useful in the

dairy industry.

Keywords: Plant rennet, Ficus johannis, Caseinolytic

activity, Milk-clotting activity


25

A spectroscopic and molecular docking

approach to investigate the interaction of

thioridazine with ct-DNA

Samaneh Rashtbari, Reza Yekta, Gholamreza Dehghan*



Study the interaction of various drugs with DNA is the

mainapproach to new drug design. In this work, the

interaction of thioridazine (TR), an antipsychotic

medication, which is widely used to treat psychotic

disorders such as schizophrenia was investigated with calf

thymus DNA (ct-DNA) using spectroscopic methods

including UV-visible absorption and fluorescence

spectroscopy along with molecular docking studies and

viscosity measurements. The binding constant (Kb) value

was calculated to be 5.3 ×103 M

-1, that is comparable to the

Kb calculated for groove binders. Competitive binding

study with methylene blue (MB) showed that TR cannot

be substituted for the MB probe (an intercalator agent),

confirming the groove binding of TR. Viscometric studies

show a slight change in the viscosity of the ct-DNA, which

suggests TR binds to DNA through a groove-binding

mode. Molecular docking studies confirmed that TR can

form complex with DNA and this drug interact with DNA

through groove binding mode. These outputs are in good

agreement with experimental results and all the results

indicated that TR is fitted into the minor groove of ct-

DNA.

Keywords: Thioridazine, Calf thymus DNA, Groove

binding, Molecular docking

Effects of farnesiferol A on structure and

activity of bovine liver catalase: using

experimental and simulation methods

Samaneh Rashtbari, Reza Yekta, Gholamreza Dehghan*



Farnesiferol A as a natural sesquiterpene coumarin

includes a variety range of pharmacological and biological

activities. In the present study, effects of farnesiferol A on

the structure and activity of bovine liver catalase (BLC)

were assessed by different spectroscopic and simulation

methods. Kinetic studies showed that farnesiferol A has a

significant inhibitory activity on BLC in a non-competitive

manner. UV–vis absorption, CD spectroscopy,

synchronous fluorescence and fluorescence spectroscopy

investigations indicated conformational changes in the

structure and active site of BLC in the presence of various

concentrations of farnesiferol A. Fluorescence studies

showed that farnesiferol A is able to quench intrinsic

emission of BLC through the static type of quenching. The

obtained data in the thermodynamic analyses suggested

that electrostatic interactions have a major role in the

binding process of farnesiferol A on BLC. Furthermore,

the role of Tyr 357 residue in the mechanism of inhibition

was recognized by simulation methods.

Keywords: Catalase, Farnesiferol A, Non-competitive

inhibition, Simulation



26

Binding interaction of perphenazine with calf

thymus DNA: a spectroscopic and molecular

docking study

Reza Yekta, Samaneh Rashtbari, Gholamreza Dehghan*



In this work, study the interaction of ct-DNA (calf thymus

DNA), as a main intracellular target for various small

molecules, with perphenazine (PP), an antipsychotic agent,

was performed using different techniques including

fluorescence spectroscopy, UV-vis absorption

spectroscopy, viscometry measurement and molecular

docking studies. UV–vis absorption spectroscopy results

revealed that the interaction of PP with ct-DNA is a groove

binding mode and the binding constant (Kb) value was

calculated to be 8 × 103 M

-1. Competitive fluorimetric

studies with methylene blue (MB) displayed that PP

possibly fits into the minor groove of ct-DNA. The results

of viscometric studies indicated that no significant change

occurs in the length of ct-DNA and confirm the groove

binding mode of PP with ct-DNA. All experimental results

were confirmed by molecular docking outputs.

Keywords: Perphenazine, Groove binding, Molecular

docking, Calf thymus DNA

Oxytocin stands against 3-NP induced HD

like disease in male and female rats

Mehdi Moslemi*, Fariba Khodagholi, Fereshteh Motamedi Neuroscience Research Center, Shahid Beheshti University of Medical

Sciences, Tehran, Iran


Several studies implicate the role ofOxytocin (OXT ( in

anti-oxidative and anti-apoptotic pathways that are

involved in various pathophysiological processes but these

effects can vary depending on sex and environmental

circumstance. Therefore, this study examined the effects of

OXT on learning and memory impairment induced by 3-

nitropropionic acid (3-NP) in Huntington like a model.

Furthermore, acetylcholinesterase (AChE) activity, the

enzyme that degrades the neurotransmitter acetylcholine

which is important for learning and memory function, was

also evaluated in the Hippocampus (HIP) of male and

female rats. In this regard, three-month-old male and

female rats were injected intracerebroventricular OXT

(10µg) and the next day 3-NP injected for 5 days

(20mg/kg). The results of this study showed that oxytocin

had no effect in improving motor dysfunction caused by 3-

NP in male and female rats but illustrated that OXT

improved learning and memory impairment caused by 3-

NP in both sexes. In a way that the time latency in step-

through passive avoidance task reached from 200 ±10 s, in

male and 210 ± 10 s, in female control groups to 40 ±10 in

male and 45 ±10 in female rats in 3-NP injected ones. In

these conditions, OXT injection increased the time latency

to 150 ±10 in male and 100 ±10 in female rats. Besides

OXT caused a decrease in the activity of the AChE in

different brain regions. In a way that the amount of

enzyme activity in the HIP reached from 12±2 in male and

12±2 in female control groups to 18 ±2 in male and 16 ±2

in female rats compared to 3-NP injected ones. In this

case, oxytocin injection reduced this amount to 15±2 in

male and 10±2 (µM/min/mg tissue) in female rats.

Collectively the obtained data determined the protective

effect of oxytocin in HD like a disease.

Keywords: Oxytocin, Acetylcholinesterase, 3-NP,

Learning, memory


27

Comparative studies on the interaction

between glycerol polyol with bovine trypsin:

spectroscopic and theoretical approaches

Lida Momeni*, Nabat Naqshbandi

Department of Biology, Faculty of Sciences, Payam Noor University, Iran


The aim of the present investigation was to study how

polyols could affect the structure, stability, and the activity

of the protease. Different spectroscopic methods, kinetics,

and molecular dynamics (MD) studies were carried out to

study the effect of glycerol on the activity and structure of

trypsin in 50 mM Tris–HCl buffer.It was demonstrated

that polyol quenched the intrinsic fluorescence of trypsin

by the static quenching process. The calculated

thermodynamic parameters (ΔH°<0, ΔS°<0) showed that

the acting forces of complex formation between trypsin

and polyol were hydrogen bonds and van der Waals forces

with an overall favorable Gibbs free energy change

(∆G°<0). The increase in the absorption of trypsin in the

presence of glycerol was as a result of the formation of the

glycerol-trypsin complex. In addition, the kinetic studies

revealed that this polyol enhances enzyme activity of

trypsin, in a concentration-dependent manner. Also,

molecular docking, as well as thermodynamic parameters,

indicated that hydrogen bonds and van der Waals forces

play important role in stabilization of trypsin- polyol

complexes. Molecular docking results showed the

presence of one binding site on the surface of trypsin with

a negative value for the Gibbs free energy (∆G˚) of the

binding of glycerol to trypsin. Near-UV and Far-UV

circular dichroism studies also demonstrated the transfer of

Trp, Phe, and Tyr residues to a more flexible environment.

Keywords: Bovine trypsin, Polyol, Enzyme activity,

Intrinsic fluorescence, Molecular docking

A comparison of flavonoid and phenolic

content of different parts of Eryngium planum

Zeinab Fadaei1, Moslem Afsharnezhad2, S. Shirin Shahangian2*, Majid Rajabiian1, Reyhaneh Sariri2

1 Department of Biology, Payamnoor University, Mashad Branch,

Mashad, Iran 2 Department of Biology, Faculty of Sciences, University of Guilan,

Rasht, Iran


Chochagh is a local name for Eryngium planum which can

grow widely in Gilan province. The plant contains

limonene, alpha pinnen,and folic acid. Besides, tannins

and sucrose are also present in its roots. Traditionally, it is

used to balance the appetite, as antibacterial/antifungal, to

ease pains from arthritis and reduce inflammation, to

increase breast milk and as a natural diuretic. It is also

used as a traditional food flavoring. Here, we investigate

and compare the antioxidant activity in different parts of

the plant. Ethanol extracts from various parts of the plant

were first prepared. Aluminum chloride colorimetry and

Folin–Ciocalteu reagentswere used to measure the total

flavonoid and phenol contents, respectively. In brief, after

adding the reagents to each extract, the absorption was

measured at 514 and 720 nm, respectively. It was found

that all parts of the plant contained flavonoids and phenols.

However, the flavonoid and phenol content of leaves was

significantly higher than all other parts. The traditional

medicinal plant, chochagh, possesses a considerable

antioxidant activity in the form of flavonoids and phenols.

It can, therefore, be considered as a possible candidate for

use in pharmaceutical applications.

Keywords: Eryngium caeruleum, Flavonoids, Phenols,

Antioxidant

28

Improved thermal stability of laccase

immobilized on carboxyl functionalized

chitosan magnetic nanoparticles

Houman Maftoon, Ali Taravati

Department of Molecular and Cell Biology, Faculty of Basic Sciences, University of Mazandaran, Babolsar, Iran


Laccase is an enzyme with remarkably broad substrate

specificity and high catalytic activity, so this enzyme has a

good potential for industrial applications. However, the

industrial applications are limited due to the low stability

and poor reusability of free laccase. In an attempt to

improve the thermal stability of laccase, a novel

immobilized laccase on carboxyl functionalized

chitosan/Fe2O3 magnetic nanoparticle with high activity

was synthesized and characterized by transmission

electron microscopy, Fourier-transform infrared

spectroscopy (FTIR) and X-ray diffraction (XRD). The

optimal conditions for laccase immobilized onto

carboxychitosan/Fe2O3 magnetic nanoparticle were 20 mM

of 1-(3-(dimethylamino) propyl) 3-ethylcarbodiimide

hydrochloride (EDC), 400 mM of N-hydroxysuccinimide

(NHS), 100 µl enzyme solution (approximately 0.5 U/mL)

and 24 h immobilization time. Based on our results, the

optimum temperature for free and immobilized laccase

was found at 50ºC and 55

ºC, respectively. So, the

immobilized laccase showed enhanced resistance to

thermal denaturation compared with free laccase. Results

of this study demonstrated that the immobilized enzyme

could be used in many applications due to the better

immobilization efficiency and thermal stability of

immobilized laccase.

Keywords: Chitosan, Magnetic nanoparticle, Laccase,

Thermal stability

Evaluation of cystatin-C by ELISA assay in

rats with diabetes mellitus supplemented by

zinc oxide nanoparticles

Roya Salari Moghadam*, Shahin Ehtesham Far

Department Biology, Faculty of Science, Islamic Azad University of

Bonab, Iran


Diabetes mellitus is a metabolic disorder with an essential

property in increasing plasma glucose that finally extra

glucose is excreted by urine and ends up losing an

important source of energy. In this disorder metabolism of

lipids and proteins are disturbed as well. In the present

study effects of oral administration of zinc oxide

nanoparticles (ZnO-NPs) in different doses were assessed

on diabetes mellitus compared with control rats. 120 with

Wistar male rats were randomized into two groups, healthy

and diabetic rats (n=60). The diabetes was induced

intraperitoneal by using streptozotocin in a dose of 45 mg

per each kg of weight mass. Rats were attendant by ZnO-

NPs with (1-3-10) mg dose and a bloodsample was taken

in 7-7-56 days and these levels measured in the mentioned

days. Glucose level by using the enzymatic colorimetric

method, Insulin by ELISA, Cystatin-c by Latex Enhanced

Immunoturbidometric, creatinine by an enzymatic

colorimetric method without elimination of proteins were

assayed. Statistical analyses were performed using SPSS

Version 22. Normalization of data were performed using

Kolmogorov-Smirnov and Tukey’s test and the level of

significance was set (p<0.05). The results of the present

study showed that oral administration of ZnO-NPs e in

various doses significantly decrease in glucose, urea,

creatinine and cystatin-c levels and significant increase of

insulin in comparison with control group (P<0.01), most

alteration in compare to other groups was observed in

diabetic rats that administrated by ZnO-NPs 10 mg.

Induction of diabetes mellitus along with oxidative stress

induction resulted in nephropathy in rats. This was

accompanied by an increase in urea, Cystatin-c.

Administration of ZnO-NPs reduced blood glucose levels

and inhibited oxidative stress, hence, prevented renal

failure in rats.

Keywords: Diabetes, Nephropathy, Zinc oxide

nanoparticles, Cystatin-c

29

Evaluation of Zinc level in the serum of hypo

and hyperthyroidism patients in Urmia

County

Roya Salari Moghadam1, Siamak Asri Rezayi2 1 Department Biology, Faculty of Science, Islamic Azad University of Bonab, Iran 2 Department Clinical Pathology, Faculty of Veterinary, University of

Urmia, Iran * Coresponding author: [email protected]

Zinc plays a crucial role in biochemical reactions and the

immune system, and it is an important part of

metalloenzyme, translation factors, and other proteins.

Elemental Zinc is involved in several functions in organs

including endocrine regulation and secretion, thyroid

hormones and Insulin. This element is required for the

accurate function of 5' Iodotyrosine deiodinase type 1 for

conversion of thyroxine (T4) to active triiodothyronine

(T3) and triiodothyroninereceptors for biological activity.

In this cross-sectional study, 120 patients were randomly

entered to the study. The patients, 43 male and 77 female

average age of 38 ±16, were randomly divided into

hypothyroid group, hyperthyroid and control groups

(n=40).3 mL venous blood sample was taken and after

serum division, hormone levels of T3, T4, TSH and

elemental zinc were analyzed by ELISA and chemical

methods, respectively . Statistical analyses were performed

using SPSS Version 22. Normalization of data was

performed using Kolmogorov-Smirnov and Tukey’s test

and the level of significance was set (p<0.05). The

findings of the present study showed that in the

hypothyroid group there was a significant increase in TSH

serum level (P<0.001) and a significant decrease in serum

levels of T4 and zinc (P<0.001). No significant difference

was observed in serum level of T3. In the

hyperthyroidgroup, there was a significant increase in TSH

serum level (P<0.001) and a significant increase in serum

levels of T4 and zinc (P<0.005).No significant difference

was observed in serum level of zinc. This study showed

parallel results with other researchers who showed there

was significant decrease in hypothyroid patients zinc value

in comparison with the controlgroup. One of the

explanations for these results is that zinc absorption in

hypothyroid patients is disrupted and zinc deficiency

causes disorders in 5'Iodotyrosin deiodinase type ı activity

and deiodination of thyroxine to triiodothyronine. In this

study zinc level of hyperthyroidism, patients did not show

the significant change that was in agreement with the

results of other researchers which reported a significant

increase. Zinc levels in hypothyroidism and

hyperthyroidism showed no significant changes. This was

in agreement with the results of others. It seems that

elemental zinc supplementation may improve the function

of the thyroid gland in hypothyroid patients.

Keywords: Hypothyroid, Hyperthyroid, Tiroxin,

Triiodothyronine, Zinc

Immobilization of laccase enzyme on Iron

Oxide nanoparticle and determination of its

activity

Kiyanoush Bakhshande, Ali Taravati*, Maryam Mohajerani

Department of Molecular and Cell Biology, Faculty of Basic Sciences, University of Mazandaran, Babolsar, Iran


Immobilization of enzymes improves their properties for

efficient utilization in various processes. Iron oxide

nanoparticle is appropriate for different functions due to its

valuable specification such as availability,

inexpensiveness, nontoxicity, easy separation and high

surface-to-volume ratio. In this study, iron oxide

nanoparticle used in immobilization of laccase enzyme.

This magnetic nanoparticle was prepared using co-

precipitation method from inorganic salts of Fe (III) and

Fe (II) with a stoichiometric ratio of 2:1 (Fe3+

/Fe2+

). This

magnetic nanoparticle activated by 400 mM of N-

Hydroxysuccinimide (NHS) and 20 mM of 1-Ethyl-3-(3-

dimethylaminopropyl) carbodiimide (EDC) and

eventually, laccase enzyme (0.025 g/ml) loaded into the

solution pH of 5.5 and immobilization process

accomplished by incubation lasting 24hours at room

temperature.Under these optimum circumstances, laccase

enzyme immobilized onto iron oxide nanoparticle by

efficiency up to 90%.Overall, iron oxide nanoparticle is a

proper support for immobilization of laccase and other

enzymes.

Keywords: Iron oxide, Magnetic nanoparticle, Enzyme

immobilization, Laccase


30

Thiolation of Chitosan nanoparticle and

immobilization of Laccase enzyme

Seyede Tahere Mir-Salehi1, Ali Taravati1*, Fatemeh Tohidi2 1 Department of Molecular and Cell Biology, Faculty of Basic Sciences, University of Mazandaran, Babolsar, Iran 2 Department of Medical Biotechnology, Faculty of Medicine, Babol

University of Medical Sciences, Babol, Iran * Corresponding author: [email protected]

Laccase has a lot of importance in biotechnology and

many industrial applications.Immobilized enzyme present

enhanced activity and storage time, pH and thermal

stability compared with free enzyme. In this study,

Laccase enzyme immobilized onto the thiolated chitosan

nanoparticle. The thiolated chitosan nanoparticle

characterized by X-ray diffraction (XRD) and Fourier

transform infrared spectroscopy (FTIR). Chitosan

nanoparticle was synthesized using sodium

tripolyphosphate (TPP) as a polymerizing agent. Thiolated

chitosan nanoparticle was prepared by adding thioglycolic

acid (TGA). Finally, to performing immobilization,

laccase enzyme (0.025 g/ml) loaded onto thiolated

chitosan nanoparticle in the solution with pH of 5.5 by 24

hours incubation time at room temperature. Under these

optimum circumstances, the efficiency of laccase

immobilization was on the range of 42-48%. Based on our

results, thiolated chitosan nanoparticle can be used as an

ideal support for laccase immobilization in the

biotechnological application.

Keywords: Chitosan, Thiolated nanoparticle, Laccase,

Enzyme immobilization

Immobilization of α-amylase on nanoporous

zeolite: improved stability and reusability

Leila Sadeghi1*, Vahid Yousefi Babadi2

1 Department of Animal Biology, Faculty of Natural Science, University of Tabriz, Tabriz, Iran 2 Department of Physiology, Payam Noor University of Iran, Iran


α-Amylase is one of the important biocatalyststhat

hydrolysis of internal α-1,4-glucosidic linkage in starch

and related oligosaccharides that widely used in the food

and detergent industry. Enzymes are sensitive against

harsh condition such as acidic pH and heating and could

not be recovered also. These restrictions could be

improved by enzyme immobilization that converts water

soluble enzymatic proteins into the solid form.

Nanoporous zeolite with special features such as

mechanical and thermal stability, non-toxicity, high

stability against microbial attack and insolubility in

organic solvents could be used as a matrix for enzyme

immobilization. This study immobilized α-amylase from

Aspergillus oryzaeon the nanoporous zeolite bed with

aldehyde group adding method. Free and immobilized α-

amylase enzyme activity was measured by the

dinitrosalicylic acid method at 450 nm with starch as

substrate. Nanoporous zeolite was activated by

Glutaraldehyde and the yield of covalent immobilization

calculated as 59.34 . Optimum temperature range for

free and immobilized enzyme activity measured between

45-55 C and optimum pH assessed 8-8.5. Thermostability

of immobilized enzyme was significantly increased rather

than free enzyme and stability against high pH also was

more for fixed enzyme. Storage stability in 4C and thermal

stability in 65 C for immobilized enzyme estimated more

than free enzyme (near to 5 folds), which is important for

industrial application of enzymes. Our immobilized

enzymes are reusable and could be recovered 10 times

without significant decrease in starch hydrolyzing ability.

Improved functional stability of immobilized enzyme

makes it as an economic enzyme in industry.

Keywords: α-Amylase, Functional stability,

Thermostability, Storage stability

31

Measurement of 17α-hydroxyprogesterone

(17α-OHP) by using solid-phase extraction

and HPLC method

Farahnaz Asadi1, Maryam Monsef Shokri2, Maryam Ghobeh1, Yousef

Abdossalami3* 1 Department of Biology, Science and Research Branch, Islamic Azad

University, Tehran, Iran. 2 International Sturgeon Research Institute, Agricultural Research, Education & Extension Organization (AREEO), Rasht, Iran. 3 Department of Occupational and Environmental Health, Faculty of

Health, Iran University of Medical Sciences, Tehran, Iran. * Corresponding author: [email protected]

Hydroxylase enzyme deficiency is one of the most

common forms of congenital adrenal hyperplasia.

Depending on the amount of enzyme deficiency, clinical

symptoms may be severe or if the enzyme deficiency is not

significant, the clinical symptoms would be manifested

during puberty. The best way to diagnose this disease is to

measure serum 17α-hydroxyprogesterone (17α-OHP)

marker in infants' blood. In this study, a new method for

the detection of 17α-OHP metabolite has been introduced.

For this purpose, the solid phase extraction method

combined with liquid chromatography has been applied.

First, the effective processes on the efficiency of the

proposed method such as solvent type, sample volume,

extraction solvent volume, and salt percentage were

identified and optimized. Then, 17α-OHP was measured

using HPLC. After optimization, the detection limit was 4

ng/ml and the measurement was achieved linearly in the

range of 10 to 5000 ng/ml. The results indicate that this

method can be used to measure the plasma concentration

of the intended analyte in infants with congenital adrenal

hyperplasia.

Keywords: Congenital adrenal hyperplasia, 17α-

hydroxyprogesterone, Solid phase extraction, HPLC

The improvement of the protein profiling of

Ailanthus altissima pollen extract using high-

length immobilized pH gradient as the first

dimension for 2-DE

Fatemeh Mousavi1*, Yousef Shahali2

1 Space Biology and Environment center, Aerospace Research Institute, Ministry of Science Research and Technology, Tehran, Iran

2 Razi Vaccine and Serum Research Institute, Agricultural Research,

Education and Extension Organization (AREEO), Karaj, Iran. * Corresponding author: [email protected]

Two-D gel electrophoresis (2DE) is known as a powerful

technique for the separation of individual proteins from

complex samples based on their isoelectric points and

molecular weights. This technique expands the number of

proteins that could be identified. Recently, coupling 2-DE

with immobilized pH gradients has provided higher

resolution of this method. The aim of this study was the

improvement of the protein profiling of Ailanthus

altissima pollen extract using 18 cm IPG strips (pH 3–10

nonlinear). For this purpose, the pollen proteins were

extracted in phosphate-buffered saline (PBS). Dialysis

is used to remove salts from protein solutions. The protein

content of A. altissima pollen extract was studied using the

Bradford protein assay followed by a 2-DE.

Approximately 400 µg A. altissima protein extracts

wasadded to the sample rehydration buffer. Results showed

that the total protein concentration of AP extract was 4.3

μg per μl. Two-D electrophoresis of AAP extracts revealed

400 protein spots distributed in a wide range of pI and

molecular masses. However, our previous study using 7

cm IPG strips (pH 3–10 nonlinear) allowed the detection

of only 125 protein spots. Therefore, IPG strip length and

gel dimensions significantly influence the resolution and

sample throughput on two-dimensional gels and proven

experience the maximal resolution is obtained using 18

and 24 cm IPG strips.

Keywords: 2D gel electrophoresis, Strip, Protein,

Dialysis, Pollen, Protein profile


32

Alpha-glucosidase inhibitory activity by

hexane extract from different aerial parts of

plants, Haplophyllum acutifolium DC. and

Ferula haussknechtii Wolff ex Rech

Hero Ghafaryan, Mohammad Ali Zarei*

Department of Biological Sciences, Faculty of Science, University of Kurdistan


The dietary carbohydrates should first be broken down to

monosaccharides by some gastrointestinal enzymes since

only monosaccharides can be absorbed from the intestinal

lumen. α-Glucosidase is the key enzymes involved in the

digestion of carbohydrates. Liberated glucose is then

absorbed by the gut and results in postprandial

hyperglycemia. The control of postprandial hyperglycemia

is an important strategy in the management of diabetes

mellitus. Since the effect of hexane extracts of

Haplophyllum acutifolium DC. and Ferula haussknechtii

Wolff ex Rech has already been demonstrated, the purpose

of this study is to investigate and identify organs of the

above plants that show maximum inhibitory activity, as

well as the measurement of the antioxidant activity of

these organs. Hexane extracts of isolated organs of the

plants were prepared using a rotary evaporator. The

inhibitory effects of the extracts were investigated in six

concentrations at 405 nm wavelength using a microplate

reader. Antioxidant activity of the extracts was measured

using the DPPH scavenging test. The highest inhibitory

activity of Ferula haussknechtii Wolff ex Rechwas

observed at the 100 mg/ml concentration (100% inhibition

and IC50= 0.0001mg/ml). The most inhibitory activity

ofHaplophyllum acutifolium DC.was related to a 1000

mg/ml concentrationflower organs(100% inhibition and

IC50 = 0.01 mg/ml) and leaf. (100% inhibition and IC50 =

0.06 mg/ml). Haplophyllum acutifolium DC.organs have

antioxidant activity of 100% and EC50 = 6.21mg/ml,

EC50 = 2.37mg/ml for flower and leaf respectively. Ferula

haussknechtii Wolff ex Rech leaves and stem also have

100% antioxidant activity and EC50 = 0.96 mg/ml EC50

=22.4 mg/ml respectively. The results of this study

indicate that the hexane extract of these two organs

possesses effective inhibitors, and the attempt to isolate

these materials and their interactions with the enzyme is a

good subject for future research with the aim of accessing

inhibitors with pharmaceutical uses.

Keywords: α-Glucosidase, Inhibitor, Hexane extract,

Haplophyllum acutifolium, Ferula haussknechtii

Investigation of covalent attachments between

functionalized tungsten disulfide and anti-

Hepatitis B antibody

Mostafa Shourian* , Zeinab Soleimani sardo

Department of Biology, Faculty of Sciences, University of Guilan, Rasht, Iran


Hepatitis B can cause two types of acute and chronic

illness and cause damage to the liver and even death. So

the early diagnosis of this virus is very effective. To

enhance the signals and to increase the sensitivity and

detection of hepatitis B viruses, the active tungsten

disulfide mineral nanotubes are used.Tungsten disulfide

nanoparticles (INTs-WS2) are used to polycarboxylate or

polyaminoisation the surfaces of these nanotubes, which is

responsible for the solubility of these nanotubes in

conventional solvents.In fact, PolyCoH f -INT-WS2 was

created by polycarboxylation, which uses the Vilsmeier-

Haack electrophilic property (VH). In this method, the

mixture containing DMF (secondary N-formyl amine), O-

alkylating 2-Bromo-acetic acid (2-BrCH2COOH), and

silver acetate (Ag (L) OAc) as a bromophilic agent to

enhance abstracting the halogen from bromo acetic acid, is

used for polycarboxylation of INT-WS2. The DMF and

the ionization of silver bromophyll cations are very

necessary, and their absence prevents polycarboxylation.

In this test, the binding of anti-hepatitis B virus antibody to

the functionalized WS2 nanotubes was performed by

creating a covalent bond between carboxyls at the surface

of the WS2 nanotubes functionalized, and a surface amine

of lysine or arginine of anti-hepatitis B virus antibodieswas

conducted. This method can be a useful and effective way

to detect the hepatitis B virus faster and help people with

the disease.

Keywords: Functionalized tungsten disulfide, Anti-

Hepatitis B antibody, covalent attachment



33

Study of the beneficial effects of Tsukamurella

inchonensisin STZ induced type I diabetic rat

intestine

Monire Khordadmeh1, Mehran Mesgari Abbasi2 , Solin Ghaderi1*,

Katayoon Nofouzi1 1 Department of Pathobiology, Faculty of Veterinary Medicine,

University of Tabriz, Iran 2 Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran


This study was performed to evaluate the probable

protective effects of Tsukamurella inchonensis (Ti), as

heat-killed Actinomycetales, on the histological and

biochemical changes of Wistar rat after experimental

diabetes mellitus induced by intraperitoneal injection of 55

mg/kg streptozotocin (STZ). The experiment was carried

out by oral administration of Ti at two doses of 105 (low

dose) and 107 (high dose) mg/kg body weight in ten rats

(in each group) continuously for 14 days. The third group

received normal saline as a diabetic control group and the

last group mentioned as a negative control (no diabetic, no

treatment). The effects of this bacteria were determined on

21st days post study by histopathological examination of

the small intestine with a common method. Moreover,

biochemical oxidative stress parameters including MDA

(Malondialdehyde), SOD (Superoxidase dismutase) and

CAT (Catalase) were evaluated on the intestinal tissue. In

the present findings, significant differences were observed

in the pathological lesions such as degenerative changes in

the intestinal mucosa, capillary congestion, submucosal

edema and inflammatory cells infiltration which were less

severe in both treatment groups, particularly in high dose

group. Additionally, MDA, SOD and CAT measurement

showed significant differences in both treatment groups

compared to diabetic rats. On the basis of the current

results, it seems that oral administration of Ti (particularly

in high dose) can improve tissue damage induced by

diabetes mellitus in Wistar rat. In conclusion, the present

results suggest that the heat killed Tsukamurella

inchonensiscan have protective effects on diabetes mellitus

complications.

Keywords: Tsukamurella inchonensis, Diabetes mellitus,

Pathological lesions, Biochemical changes

Study of the protective effects of Gordonia

bronchialisin heart injury of diabetic rats

Monire Khordadmeh1, Mehran Mesgari Abbasi2, Solin Ghaderi1*,

Hossein Tayefi Nasrabadi3, Katayoon Nofouzi1 1 Department of Pathobiology, Faculty of Veterinary Medicine,

University of Tabriz, Iran 2 Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran 3 Department of Basic Sciences, Faculty of Veterinary Medicine,

University of Tabriz, Iran * Corresponding author: [email protected]

The present study was conducted to evaluate the protective

effects of Gordonia bronchialis (Gb), as heat-killed

Actinomycetales,on the biochemical and histological

changes in Wistar rat after experimental diabetes mellitus

induced by intraperitoneal injection of 55 mg/kg

streptozotocin (STZ). The experiment was performed by

oral administration of Gb at two doses of 105 (low dose)

and 107 (high dose) mg/kg body weight in ten male rats (in

each group) continuously for 14 days. The third group

received normal saline as a diabetic control group and the

last group mentioned as a negative control (no diabetic, no

treatment). The effects of this bacteria were determined on

21st days post study by histopathological examination of

the heart with a common method. Moreover, biochemical

parameters including CK (Creatinine Kinase), serum

glucose level, cholesterol, and triglyceride were measured

on the blood samples. In the present results, significant

differences were observed in the pathological lesions such

as degenerative changes in myocardial cells, focal

hemorrhage, capillary congestion and edema which were

less severe in both treatment groups compared to the

diabetic group. Additionally, CK, serum glucose level,

cholesterol, and triglyceride measurement showed

significant differences in both treatment groups compared

to diabetic rats. On the basis of the current findings, it

seems that oral administration of Gb can improve heart

damage induced by STZ-induced diabetes mellitus in

Wistar rat. In conclusion, the present results suggest that

the heat-killed Gb can have beneficial effects on diabetes

mellitus complications.

Keywords: Gordonia bronchialis, Diabetes mellitus,

Pathological lesions, Biochemical change


34

Evaluation of the inhibitory effect of

Trachyspermum copticum fractions on

AGEformation in the diabetic model on in

vitro

Fatemeh Golshahi*, Seifollah Bahramikia

Department of Biology, Faculty of Basic Sciences, University of Lorestan, Iran


Increasing the level of intracellular glucose leads to the

formation of advanced glycation end products (AGEs)

through non-enzymatic glycation in intracellular and

extracellular proteins, and as a result, proteins lose their

normal function. Active oxygen species are involved in the

process of glycosylation of proteins. Therefore, in this

study, we investigated the antioxidant activity and

inhibitory effect of Trachyspermum copticum organic

fractions on the formation of AGE compounds in the

diabetic model on in vitro.The phenolic and flavonoid

content of the organic fractions was measured by Folin-

Ciocalteu and Ammonium Chloride method, and their

antioxidant activity was measured by trapping DPPH

radicals. Hydroxyl radicals were produced by sugar

autoxidation and measured by benzoate hydroxylation.

The diabetic model was designed with glycation of BSA

(bovine serum albumin) on in vitro. AGE formation was

measured by fluorescence absorption at the excitation and

emission maxima at 335 nm and 385 nm, respectively. Our

results show that the fraction of ethyl acetate of

Trachyspermum copticum at different concentrations (10-

500 μg/ml) has the highest antioxidant activity, phenolic,

and flavonoid content, respectively, IC50: 7.9 μg / ml,

275.7 ± 3 and 175.7 ± 3.2 mg of gallic acid per gram of

dried extract as well as the highest inhibitory effect on the

formation of AGE compounds and autoxidation of sugars.

Keywords: Advancedglycation endproducts, Diabetes,

Trachyspermum copticum, Antioxidant Activity

Determination of the effect of

cinnamaldehyde of cinnamon on the urease

activity

Boshra Mohammadi Ahmadvandi*, Maliheh Sadat Atri, Maryam

Mohajerani Department of Molecular and Cell Biology, University of Mazandaran,

Babolsar, Iran


Urease-producing bacteria have a negative effect on

human health and the environment. Forexample,

Helicobacter pylori is a pathogenic bacterium that is

associated with infection and peptic ulcer. Pathogenicity is

mediated by urease. Cinnamaldehyde is the most important

compound of cinnamon essential oil, which has anticancer

and antimicrobial properties. Cinnamaldehyde effect on

the urease activity is examined in this study. The activity

of urease in the presence and absence of cinnamaldehyde

was measured spectrophotometrically by microplate reader

during two minutes which was required for releasing

enough ammonia to raise the pH of the assay mixture.

Phenol red was used to indicate ammonia production with

a color change from yellow to pink that is measured at the

wavelength of 570 nm. Cinnamaldehyde inhibited the

activity of urease after 5 minutes of preincubation which

caused an increase in the Km value while the Vmax value

remained constant. Inhibition decreased as a result of the

substrate concentration rise. Consequently, in this study

cinnamaldehyde is described as a competitive inhibitor of

urease. Inhibition of urease enzyme by cinnamaldehyde

may play an important role in the antimicrobial activity of

cinnamon extract as well as in cinnamon's medicinal

properties against peptic ulcer.

Keywords: Urease, Cinnamaldehyde, Enzyme assay,

Enzyme competitive inhibition

35

Inhibition of urease activity by the main

chemical component of clove oil

Boshra Mohammadi Ahmadvandi*, Maliheh Sadat Atri, Maryam

Mohajerani Department of Molecular and Cell Biology, University of Mazandaran,

Babolsar, Iran


The aim of this study was the elucidation of the inhibitory

influence of eugenol, the principal chemical component of

clove oil on the activity of urease. Urease activity can be

the cause of some disease and pathogen infections

including Helicobacter pylori which causes peptic ulcers.

More than 50% of the populations harbor H. pylori. The

increasing resistance of bacteria towards antibiotics is

problematic and as an alternative, effective non-antibiotic

compounds should be highly desirable since their use may

be safer and more suitable to eradicate H. pylori. In this

study, urease activity was measured by assaying the

ammonia released as a result of enzyme activity which

caused a rise in pH. Phenol red was used to indicate

ammonia production. The activity was studied for 2

minutes. As a result of ammonia release phenol red color

changed from yellow to pink that was measured in 570 nm

wavelength. Urease inhibition by eugenol was time and

concentration dependent. Inhibition raised with increasing

the concentration of eugenol and time of incubation. After

5 minutes of incubation with eugenol, the enzyme activity

was inhibited. Eugenol is described as a mixed inhibitor of

urease enzyme and showed to bean effective inhibitor of

urease and might serve as a template in the development of

urease inhibitors in pharmaceuticals.

Keywords: Urease, Eugenol, Enzyme assay, Enzyme

inhibition

Measuring ofcatalase activity in liver tissue of

mice treated with acetaminophen and

Spirulina alga

Elham Hajian Kelarijani*, Maryam Mohadjerani, Ali Taravati

Department of Cellular and Molecular Biology, Faculty of Basic Sciences, University of Mazandaran, Iran


Acetaminophen (Paracetamol) was used as 1893 to treat

pain and fever. The aim of this study was to evaluate the

effect of Spirulina algae on the catalase activity of male

mice treated with acetaminophen. In this study, 42 adult

male mice were divided into seven groups of six. The first

group, the control group that received only the standard

diet and water. The second group (sham group) which

received saline. The third group was treated with

acetaminophen (600 mg/kg b.w). The fourth group was

treated with Spirulina (600 mg/kg b.w). The fifth group

was treated with spirulina (300 mg/kg b.w). The sixth

groups of micewere treated with Spirulina 600 mg/kg b.w

plus acetaminophen. The seventh groups of micewere

treated with Spirulina 300 mg/kg b.w plus acetaminophen.

In all groups, the drug was administered through the oral

route. All animals were allowed free access to food and

water throughout the experiment which lasted for 14 days.

Twenty four hours after the last dose was administered

(had free access to water), the animals were anesthetized

with chloroform vapor, sacrificed and then the catalase

activity was measured in liver tissue. In the present study

catalase levels in the group treated with acetaminophen

showed a significant difference compared with the control

group. The sharp decline significantly in the group treated

with high doses of acetaminophen plus Spirulina,

respectively. The results showed that Spirulina at a dose of

300 mg/kg b.w, shows better protection against damage

caused by acetaminophen. The results showed too that the

use of Spirulina in combination with acetaminophen

modified catalase activity and antioxidant defense system,

which showed increased resistance to oxidative stress from

drug toxicity.

Keywords: Spirulina algae, Acetaminophen, Antioxidant

enzyme, Catalase, Liver, Mice



36

Investigation of the effect of a sedative drug,

Donepezil, on the activity of peroxidase

Niloufar Ghayoumipour, Moslem Afsharnezhad*, S. Shirin Shahangian,

Reyhaneh Sariri Department of Biology, Faculty of Sciences, University of Guilan, Rasht,

Iran


Oxidative stress, induced by free radicals, has been

implicated in the pathogenesis of many serious systemic

diseases such as diabetes, cancer and neurodegenerative

disorders such as Alzheimer’s disease. Peroxidase, as an

antioxidant enzyme, may be induced under conditions of

oxidative stress. Peroxidasesbelong to the class of oxido-

reductase enzyme that catalyzes organic and non-organic

substrates oxidation reaction. Due to the high catalytic

power and the critical role of this enzyme to eliminate free

radicals it is widely used as a model enzyme in the medical

research. This study aims to examine whether a sedative

drug for Alzheimer’s disease, donepezil, could affect the

activity of peroxidase. Peroxidase activity was measured in

a time course mode by monitoring the absorbance changes

at 510 nm, using the reaction mixture containing

aminoantipyrine/phenol/H2O2 and the enzyme. The effect

of different concentration of donepezil was evaluated on

the enzyme activity. The results showed that the drug

possesses a negligible inhibitory effect (10%) on the

enzyme activity. Donepezil as a classical drug for the

treatment of Alzheimer’s disease does not interfere with

the activity of peroxidase, as an important antioxidant

enzyme.

Keywords: Peroxidase, Antioxidant enzyme, Donepezil,

Alzheimer’s disease

Tyrosinase inhibitory and antioxidant activity

of methanolic extract from various aerial

organs of Astragalus siliquosusBioss and

Verbascum phoeniceum L.

Masomeh Babaee, Mohammad Ali Zarei*

Department of Biological Sciences, Faculty of Science, University of Kurdistan, Iran


The process of melanogenesis is responsible for the

pigmentation of the skin, eyes, and hair of human.Melanin

production is also responsible for the browning of fruits,

vegetables, and fungi. Melanogenesis begins with the

oxidation of L-tyrosine to L-Dopa by tyrosinase .The

accumulation of melanin in the skin causes complications

such as skin lesions, eczema, and melasma in humans .

Inhibition of tyrosinase could be effective in treatment of

those complications.The aim of this study was to

determine inhibitory activity on tyrosinase and antioxidant

activity of methanolic extract of various aerial parts of

Astragalus siliquosusbioss and Verbascum phoeniceum

L.Methanolic extracts of the isolated organs of the plants

were prepared using rotary evaporator. Inhibitory effects

of the extracts were evaluated in nine concentrations in 96

wells at 492 nm wavelengths using a microplate reader.

The antioxidant activity of the extracts was evaluated

using DPPH free radical scavenging index.The most

inhibitory activity of the Astragalus siliquosusBioss was at

1000 mg/ml concentration of its flower extract (97%

inhibition and IC50 = 1.58 mg/ml) and 1500 mg/ml

concentration of its stem extract (100 mg / ml and IC50 =

13.225 mg/ml). For Verbuscumphoeniceum L. the highest

inhibitory activity was observed in the 1500 mg/ml

concentration of its leaf extract (87% inhibition and IC50

= 155.3 mg/ml). All aerial parts of the Astragalus

siliquosus had 100% antioxidant activity with EC50s of

0/089, 1/775, 1.25 mg/ml for its flower, leaf, and stem

respectively. On the other hand, just leaves of the

Verbascumphoeniceumhad a 100% antioxidant activity,

with EC50 of 0.129 mg/ml. According to the results of this

study, methanolic extracts of flower and leaf organs of

Astragalus siliquosusBioss, and just leaf organ of

Verbascumphoeniceum L. had a reasonable inhibitory

effect on tyrosinase activity. So feature studies could be

focused on those organs to separate potential agents with

pharmaceutical and food industrial applications.

Keywords: Tyrosinase, Inhibitor, Methanolic Extract,

Astragalus siliquosus, Verbascumphoeniceum



37

Baicalein incorporated nanoliposome

disaggregates alpha-synuclein fibrils

Farhang Aliakbari1, 2, 3, Dina Morshedi1*, Hossein Mohammad-Beigi1, 2, 4,

Daniel E. Otzen2* 1 Institute of Industrial and Environmental Biotechnology, National

Institute of Genetic Engineering and Biotechnology, Tehran, Iran 2 Interdisciplinary Nanoscience Center (iNANO) and Department of Molecular Biology and Genetics, Aarhus University, Denmark 3 Department & Center for Biotechnology Research, School of Medicine,

Semnan University of Medical Sciences, Semnan, Iran 4 Faculty of Chemical Engineering, Tarbiat Modares University, Tehran,

Iran

* Corresponding author: [email protected],[email protected]

The aggregation and oligomerization of alpha-synuclein

(α-Syn), a protein which is available at high concentration

in the brain’s neuronal cells, is believed to play an

important role in the pathology of Parkinson’s disease.

There are several molecules which have the potential to

inhibit aggregation but a few could depolymerize mature

fibrils. Baicalein, a small molecule derived from

Scutellaria baicalensis Georgi, showed to have potential to

inhibit fibrillization. Nevertheless, its low solubility and

stability disrupt its function in the body fluid. Nowadays,

using nanoparticles as candidates for drug delivery is

welcome worldwide as they can unravel some problems

regarding drugs characters like their high hydrophobicity,

instability and low solubility in physiological fluids.

Herein, we report on the potential of baicalein

incorporated nanoliposome (NLP-Ba) to

disaggregate/depolymerize α-Syn aggregates in vitro. The

protein was undertaken during the fibrillation process and

the adopted mature fibrils were exposed to NLP-Ba either

without or with or shaking. NLP-Ba showed to have high

potential to depolymerize fibrils by using ThT fluorescens

intensity and FPLC (SuperoseTM

6 10/300 GL, Prep Grade

column). This result demonstrated that the potential of

baicalein in the NLP could still be preserved. This study

could open a new avenue in using potential but unstable

drug in neurodegenerative diseases.

Keywords: Alpha-synuclein, Baicalein, Nanoliposome,

Parkinson’s disease

The acetylcholinesterase inhibitory activity

and antioxidant properties of methanol

extract of different organs of Euphorbia

macrocladaBioss and Glaucium

grandiflorumBioss and Huet

Maryam Jokar, Mohammad Ali Zarei*

Department of Biological Sciences, Faculty of Science, University of Kurdistan, Iran


The oldest hypothesis for Alzheimer's disease, which many

available drugs have been made upon it, is the cholinergic

hypothesis. This hypothesis suggests that Alzheimer's

disease results from the reduction of acetylcholine. One of

the main reasons for acetylcholine reduction is the high

activity of acetylcholinesterase, so inhibition of this

enzyme can contribute to the control of the disease. Since

the effect of methanolic extracts of Euphorbia macroclada

Bioss and Glaucium grandiflorum Bioss and Huet has

already been demonstrated, the purpose of this study is to

investigate and identify organs of the above plants that

show maximum inhibitory activity, as well as the

measurement of the antioxidant activity of these organs.

Methanol extracts of isolated organs of the plants were

prepared using a rotary evaporator. The inhibitory effects

of the extracts were investigated in six concentrations at

405 nm wavelength using a microplate reader. Antioxidant

activity of the extracts was measured using the DPPH

scavenging test. The highest inhibitory activity of

Glaucium grandiflorum was observed at the 8 mg/ml

concentration (100% inhibition and IC50= 0.01mg/ml).

The most inhibitory activity of Euphorbia macrocladawas

related to an 8 mg/ml concentration (100% inhibition and

IC50= 1/12 mg/ml). Glaucium grandiflorum organs have

antioxidant activity of 100% and EC50 = 0/36mg/ml,

EC50 = 0/2mg/ml for flower and leaf respectively.

Euphorbium flower and leaves also have 100% antioxidant

activity and EC50=0/035 mg/ ml EC50= 0/032 mg/ml

respectively. The results of this study indicate that

methanol extract of Glaucium grandiflorum and

Euphorbium flower and leaf organs have a significant

inhibitory effect on acetylcholine aesthetic activity.

Therefore, it may be possible in feature studies of these

extracts to find potential sources of new drugs for the

treatment of acetylcholinesterase related diseases.

Keywords: Acetylcholinesterase, Inhibitor, Methanol

extract, Euphorbia macroclada, Glaucium grandiflorum



38

Synthesis of diazo dyes from 2, 6-diamino-4-

chloropyrimidine compound and the

biological evaluation of their effects on

tyrosinase activity

Mahdie Farvandi1*, Hossein Ghafouri 1, Asadollah Mohammadi2

1 Department of Biology, Faculty of Science, University of Guilan, Iran 2 Department of chemistry, Faculty of Science, University of Guilan, Iran


Tyrosinase is a membrane bound copper-containing

glycoprotein andthe binuclear center together with three

specific histidine residues forms a square-pyramidal

structure. In melanocytic cells biosynthesis of melanin

regulated by tyrosinase. In mammals tyrosinase oxidizeL-

tyrosine todopaquinone through L-3,4-

dihydorxyphenylalanine (L-DOPA). Thenthrough several

reactions Dopaquinone change into brown to black

melanin. Melanin determines the colorof human skin, hair,

and eyes. Various skin disorders result in the accumulation

of an excessive level of epidermal pigmentation. Thus,

inhibiting the formation of melanin may result in a

reduction in skin darkness. Numerous efforts have so far

been made to develop new tyrosinase inhibitors around the

world and various chemical or naturalcompounds have

been nominated.Two novel compounds based on 2,6-

diamino-4-chloropyrimidine were synthesized and

evaluated as tyrosinase inhibitors. In tyrosinase assay L-

DOPA was used as the substrate for diphenolase activity.

The reaction media for enzyme activity assay contained L-

DOPA and mushroom tyrosinase in sodium phosphate

buffer (pH 6.8) and different concentrations (10-100μM)

of inhibitors. Theabsorbance was taken at 490 nm. The

structures of the compounds were confirmed by FT-IR, H

NMR and C NMR spectroscopic techniques. Compounds a

and bbased on 2,6-diamino-4-chloropyrimidin exhibited

inhibitory potential with IC50 values 24.25 μM and 29.74

μM, respectively, as compared with standard kojic

acid.Thecompounds showed potent inhibitory

potentialsagainst mushroom tyrosinase.

Keywords: Tyrosinase, L-DOPA, Melanin, 2, 6-diamino-

4-chloropyrimidine, Inhibitor

Design, synthesis and biological evaluation of

2,4,6-triaminopyrimidine derivatives as

tyrosinase inhibitors

S. Shohreh Mirmortazavi1*, Hossein Ghafouri1, Asadollah Mohammadi2

1 Department of Biology, Faculty of Science, University of Guilan, Iran 2 Department of Chemistry, Faculty of Science, University of Guilan, Iran


Tyrosinase is a copper-dependent monooxygenase

enzyme, which is considered as the rate-limiting enzyme

in the melanin production pathway. Given this

background, melanin increment due to the tyrosinase

hyperactivity can give rise to hyperpigmentation disorders,

which is followed by wrinkles, skin irritation and melasma

in extreme cases. Besides, tyrosinase is regarded as a key

agent in the risk of malignant melanoma cancer. Therefore,

compounds inhibiting tyrosinase activity are of special

interest for clinical medicine and cosmetic industry. Two

novel derivatives of 2,4,6-triaminopyrimidine were

synthesized and confirmed by C NMR, H NMR, and FT-

IR. The inhibitory potential was evaluated based on

dopaquinone production by an enzyme assay and the

absorbance was taken at λ=490nm,

spectrophotometrically. The synthesized compounds a and

b displayed significant inhibition of tyrosinase with IC50

34.17 µM and 35.37 µM, respectively, and the activities

were compared with kojic acid.

Keywords: 2,4,6-triaminopyrimidine, Inhibitor,

Tyrosinase



39

Isolation and characterization of the c-type

lysozyme-encoding gene from Acipenser

persicus

Raheleh Hasanzadeh, S. Shirin Shahangian*, Mahmoudreza Aghamaali,

Laleh Mirzanezhad Department of Biology, Faculty of Sciences, University of Guilan, Rasht,

Iran


The innate immune system is the only and first line

defense of invertebrates and a fundamental defense

mechanism of fish. Lysozymes are critical components of

the innate immune system against bacterial infection. Due

to the less developed specific immune system of fish than

that of mammals, lysozyme has devoted considerable

attention in fish immunity. Considering their economic

importance, fish belonging to the family Acipenseridae

have long been of great interest. Persian sturgeon,

Acipenser persicus is one of the most commercially

important species in the Caspian Sea. In this study, we

report the isolation, amplification, and characterization of

chicken-type (c-type) lysozyme gene from Acipenser

persicus. Total RNA was isolated from the head kidney of

the fish using TRIzol and the complementary DNA

(cDNA) was synthesized by cDNA synthesis kit. The

cDNA was used as the template for the PCR using

degenerate primers that annealed to the conserved regions

of C-type lysozyme gene from a different genus of fish.

Analysis of the amplicon sequence revealed that the cDNA

contains an open reading frame (ORF) of 432 bp, encoding

a 143 amino acidprotein.

Keywords: Acipenser persicus, immune system, C-type

Lysozyme, cDNA synthesis, Gene amplification

New derivatives of imine as inhibitors of

acetylcholinesterase (AChE): Synthesis,

biological evaluation, antioxidant activity and

molecular docking

Samira Aslani1*, Hossein Ghafouri2, Asadollah Mohammadi 3

1 Department of Biology, University of Guilan, University Campus, Rasht, Iran 2 Department of Biology, Faculty of Science, University of Guilan, Iran 3 Department of Chemistry, Faculty of Science, University of Guilan, Iran * Corresponding author: [email protected]

Alzheimer’s diseases (AD) is a neurodegenerative disorder

of the central nervous system that connected to memory

loss, cognitive impairment. There are some drugs that

decreased symptoms of AD patients. The AD is associated

with oxidative stress condition and increased free radical,

protein oxidation in the brain of patients. AChE plays an

important role in the AD that hydrolyzes Acetylcholine

(Ach) and it is a member of the serine hydrolase family

that belonging to the α/β hydrolase. One of the

biochemical symptoms of the AD is the reduction of Ach.

Inhibitors of AChE are the most positive treatment strategy

for AD therapy that resulted in improving the cognitive

and memory. New derivatives of 2-chloro-3-hydrazino

pyrazine were designed and synthesized that show the

inhibitory activity of AChE for developing AD

therapeutics. Novel imine compounds of hydrazino

pyrazine with 4-methoxy benzaldehyde (S1), chromen-3-

benzaldehyde (S2), 3-hydroxybenzaldehyde (S3) were

produced by reflux column and purified by

recrystallization. FT-IR and NMR analysis were

performed to characterize the structure of compounds. The

inhibitory activities of S1, S2, S3 were evaluating by

Ellman’s method. Both compounds were able to reduce the

DPPH radical. They had antioxidant activity but S2 had

more antioxidant properties. Molecular docking of these

synthetic compounds was carried out and results of the

interaction between synthetic compounds and enzyme

were confirmed.

Keywords: Acetylcholinesterase (AChE), Acetylcholine

(Ach), Inhibitors



40

Adsorption and desorption studies of

cadmium by cross-linked chitosan/κ-

carrageenan

Farahnaz Dashbolaghi1, Sara Mola ali abasiyan1*, Gholam Reza

Mahdavinia2 1 Soil Chemistry Laboratory, Department of Soil Sciences, Faculty of

Agriculture, University of Maragheh, Maragheh, Iran 2 Polymer Research Laboratory, Department of Chemistry, Faculty of Science, University of Maragheh, Maragheh, Iran


The binding efficiency of cross-linked chitosan/ κ-

carrageenan for Cd2+

has been evaluated in order to

consider its application to remediate cadmium-

contaminated water. Adsorption and desorption of

cadmium by the chitosan bioadsorbent was investigated

using batch experiments. The experiments were conducted

in the definite concentration of modified chitosan (1.11 g

L-1

) with different cadmium concentrations (0- 1.97 mmol

L-1

of cadmium) at pH 7.6 and ionic strength 8mmol L-1

. In

order to determine desorption of Cd, the modified chitosan

loaded with cadmium ions was treated with 90 ml of 0.1M

EDTA. The data were described by using Freundlich and

Langmuir models. The values of the correlation coefficient

(r2) and root mean square error (RMSE) were calculated

for determining the best model. The correlation

coefficients for the adsorption of Cd2+

in water system are

0.99 and 0.99; the RMSE are 4.42 and 6.83 for Freundlich

and Langmuir equations, respectively. The correlation

coefficients for the desorption of Cd2+

in this system are

0.99 and 0.97 and the RMSE are 0.93 and 3.47 for

Freundlich and Langmuir equations, respectively. As

shown, the adsorption and desorption data at the ranged of

the Cd2+

concentration studied can be well described by

the Freundlich model. The maximum adsorption capacity

of Cd2+

onto the bioadsorbent was determined 750 µmol/g.

the results indicate that the present bioadsorbent as a great

adsorbent for Cd2+

. But, it should be noted that the

adsorbent cannot be reused for the cadmium adsorption

easily due to its low amounts of cadmium desorption.

Keywords: Chitosan, κ-carrageenan, Cadmium,

Langmuir, Freundlich

Study of cinnamaldehyde and eugenol binding

to catalase using molecular docking approach

Maede Moradi, Maliheh Sadat Atri*, Maryam Mohadjerani

Department of Molecular and Cell Biology, University of Mazandaran, Babolsar, Iran


Catalase enzyme is the main regulator of hydrogen

peroxide metabolism that degrades hydrogen peroxide and

protects cells from oxidative damages. Catalase activity

changes have been observed in some diseases such as

diabetes, Alzheimer's disease, and cancer. Studies have

shown that inhibition of this enzyme activity can help to

treat Alzheimer's and eliminate cancer cells. Cinnamon is a

well-known and commonly used spice in the food

industry. Cinnamon has several beneficial health effects

such as anti-tumor, anti-diabetes and anti-Alzheimer's

activities. Cinnamaldehyde and eugenol are the major

components of cinnamon and cinnamaldehyde allocate a

high percentage of cinnamon essential oil. According to

the therapeutic effect of cinnamon on some catalase

enzyme associated diseases, the interaction of

cinnamaldehyde and eugenol as the main bioactive

compounds of cinnamon with catalase studied in this

research. Crystal structure of catalase was obtained from

RCSB and molecular structure of cinnamaldehyde and

eugenol were downloaded from zinc database. AutoDock

tools, VMD and Ligplot used to determine the best binding

site of catalase enzyme for cinnamaldehyde and eugenol

and also for data analysis. The result elucidated that

hydrophobic interactions and hydrogen bonds play the

main role in the binding of these ligands to catalase.

Among amino acid residues involved in the interaction,

Tyr357 and His74 play important role in catalysis reaction

too. Therefore, the binding of these ligands to catalase

enzyme probably change its catalytic activity. The binding

energy of eugenol was more than cinnamaldehyde, so

eugenol stronger interaction with catalase rather than and

decreased catalase activity.

Keywords: Catalase, Molecular docking,

Cinnamaldehyde, Eugenol



41

Purification of a protease from an organic-

solvent tolerant alkalophilic Bacillus sp.

Shohreh Mohamadi, Maryam Mehrabi* 1 Department of Biology, Faculty of Sciences, Razi University, Kermanshah, Iran


Microbial proteases are important because they are able to

tolerate specific conditions, such as high temperatures,

alkaline pH and organic solvents present in various

industries. These proteases have contributed a lot to the

global enzyme market, and various types of them have

been purified from microorganisms such as Bacillus,

which have many applications due to their biological

diversity and the ease of genetic manipulation in various

fields. In this study, Bacillus sp. was isolated from the

Dehloran hot springs in Ilam, Iran, and it was grown in

Luria–Bertani (LB) medium enriched with cyclohexane

and toluene. The desired protease activity was determined

from clear zone diameter around the colonies grown in

Skim milk agar-plates (SMA) medium. The purification of

the protease enzyme was carried out using ammonium

sulfate precipitation (85%). The saturated solution was

centrifuged at 10,000×g for 10 minutes. The resulting

sediments were dissolved in a 50mM Tris-HCl buffer, pH

9 and dialyzed against the same buffer for 24h with three

times buffer replacement. The dialysate solution was

loaded onto the DEAE-Sepharose column. A salt

concentration gradient (NaCl 0-1M) was used to separate

proteins attached to the column. Purification efficiency

was checked using SDS-PAGE.Purified enzyme appeared

as a single band of molecular weight of about 27.5 kDa.

One of the prominent features of the purified enzyme is the

high activity in a broad range of pH from 4 to 11.

Furthermore, the enzyme remained active over a range of

temperature from 30 to 55 ◦C. Maximum enzyme activity

was observed at 50 °C and pH 10. The enzyme retained

20% and 30% of its activity in the presence of 10% and

30% of toluene and cyclohexane, respectively.Considering

its high activity in a broad range of pH and

temperaturetolerance and stability in the presence of

organic solvent, the purified protease may find potential

application in laundry detergents and other industrial

processes.

Keywords: Organic solvent, Protease, Bacillus sp.,

Enzyme activity

Artificial neural network for monitoring the

antioxidant status of human plasma

Hadi Ansarihadipour*

Department of Biochemistry and Genetics, School of Medicine, Arak University of Medicine, Arak, Iran


We evaluated theperformance of a mathematical method to

predict the antioxidant power of plasma and the

importance of oxidative parameters in humanplasma. One

hundred sixty-five blood samples from donors were

analyzed in this experimental study. Age, weight, and sex

were determined as demographic parameters. Albumin,

creatinine, FBS, triglyceride, uric acid and Hb absorbances

at 280 to700 nm were analyzed as biochemical parameters.

The ferric reducing ability of plasma (FRAP) and carbonyl

content of proteins (PCO) were calculated as oxidative

markers. An artificial neural network (ANN) was

developed as a multilayerfeedforward architecture using

IBM SPSS statistics. The best ANN model was performed

by a four-layer perceptron method (19-10-10-1) with

hyperbolic tangent and identity activation functions for

hidden and output layers, respectively. A significant

positive correlation (R2

=0.912) was observed between

predicted and observed values of FRAP. According to the

normalized importance, the main parameters were uric

acid (100%), oxyHb (66.8%), A560 (65%), BUN (55%),

A420 (52.9%) and creatinine (51.2%). This study

demonstrated the ability of the ANN to predict the most

important oxidative markers in human plasma.

Identification of important parameters can eliminate less

important parameters from laboratory procedures and

performs a cheaper and faster experiments. Keywords: Antioxidant status, Artificial neural network,

Human plasma



42

Design and construction of intra-chain

disulfide urate oxidase in Aspergillus flavus

Ramin Soleimani*, Mehdi Imani

Departamn Biochemistry Faculty of Veterinary University of Urmia * Corresponding author: [email protected]

The urate oxidase catalyzes the transformation of uric acid

(low solubility) into 5 hydroxyiso-urea and ultimately

alanthine (solution).The accumulation of uric acid in the

blood is prone to acute or chronic kidney disease with gout

and kidney disease. Uricase Aspergillus felucus enzymes

have been used as a therapeutic enzyme, including

reduction of urate, and in the Clinical treatment of gout

disorders, nephropathy and hyperuricemia due to

lymphatic drainage syndrome (TLS). In spite of having a

high tendency and high potential in substrate conversion to

the product, its low stability In the face of heat, its use is

limited. There are several solutions to this challenge,

including protein engineering and genetic manipulation. It

has been shown that the di sulfide bond plays an important

role in the stability of proteins by decreasing entropy of

unfolded stateThe enzymes of uricase Aspergillus flavus

have three cysteines in their normal state, but none have

the ability to form a disulfide bond.Therefore, in this

study, in order to increase the stability of the uricase

enzymes of Aspergillus flavus and to create a mutated

enzyme with new featuresone sites were modeled with the

help of MODIP server to create one new mutated enzymes.

The goal of this study is to locate the enzyme to produce a

disulfide bond. In this study, with the help of the MODIP

server, the place was chosen to be changed to the captured

Cys amino acid. Then, it was designed with the aid of the

Gene runner mutated primer software and was targeted by

the SOEing-PCR method of mutagenesis.The

bioinformatics studies showed that with the point mutation

of Ser145 and Thr185 to Cys, there was a possibility of the

formation of a di-sulfide bond. The results show that with

the help of the MODIP server, it is possible to find the

right place to create a disulfide bond. The findings from

mutagenicity suggest that SOEing-PCR can successfully

mutate

Keywords: Urate oxidase, Disulfide bond, Mutagenesis,

SOEing-PCR, Aspergillus flavus

Comparison of antioxidant properties of two

herbal carotenoids with crab (Portunus

pelagicus) hemolymph

Aliyeh Daryanavard, Saber Khodabandeh*

Department of Marine Biology, Faculty of Marine Science, University of Tarbiat Modares, Iran


Free radicals are destructive compounds that affect the

health of the cell and body.Antioxidants are useful

compounds that can react with free radicals and convert

them into harmless molecules. The human body normally

has an antioxidant defense system that is able to balance

between the body's antioxidants and free radicals by the

enzymatic antioxidants and non-enzymatic antioxidants;

the occurrence of any imbalance in this balance causes the

appearance of diseases such as cancer and early aging.

Previously, plants were the only known sources of

antioxidants, but in recent years, extensive research has

shown that many marine species are rich in beneficial

compounds, including antioxidants and anti-tumor.In the

present study, crab (Portunus pelagicus) hemolymph

isolatedwith Ethanol was used to evaluate the antioxidant

properties.The free radical inhibitory ability of crab

hemolymph was compared with two plant carotenoids

Panax ginseng and Opuntia ficus as natural antioxidants

and BHT and vitamin C as synthetic antioxidants by

DPPH method. In 1 mg/ml concentration, the free radical

inhibitory ability for vitamin C 88%, BHT 67%, crab

hemolymph 62%, ginseng 57%, and Opuntia ficus 41%

were obtained and in 0.5 mg/ml concentration, for Vitamin

C 71%, BHT 50%, crab hemolymph 44%, ginseng 42%

and Opuntia ficus 14% were obtained. The hemolymph

isolated from crab showed a relatively good free radical

inhibitory ability compared to herbal and synthetic

antioxidants, which can be considered in studies of marine

antioxidants.

Keywords: antioxidant activity, DPPH, hemolymph,

Portunus pelagicus


43

Extraction of saponins fromTribulus terrestris

and evaluation of its effects on human serum

albumin (HSA) structure by UV-

visibleandFT-IR spectroscopies

Roya Boroumand Gohar1, Azadeh Hekmat1*, Maryam Monsef Shokri2,

Kambiz Larijani3 1 Department of Biology, Faculty of Science and Research, Islamic Azad

University, Tehran, Iran 2 International Sturgeon Research Institute, Agricultural Research, Education & Extension Organization (AREEO), Rasht, Iran 3 Department of Chemistry, Faculty of Science and Research, Islamic

Azad University, Tehran, Iran


Tribulus terrestris is a medicinal plant, which widely

distributed around the world. Saponins from this plant are

responsible for its biological activities. Saponins as

secondary metabolites that are found in many plants and

some animals are high molecular weight glycosides,

consisting of a sugar moiety linked to a triterpene or

steroid aglycone.Saponins have cytotoxic, pro-apoptosis

and antimetastatic effects on cancer cells. Furthermore, the

total saponins extracted from the plant have beneficial

effects on various ailments such as urinary tract infections,

inflammations, leucorrhea, edema, and ascites.In this

research after extraction of Tribulus terrestrisfractions,

saponins were isolated by vacuum liquid chromatography

(VLC) and thin layer chromatography (TLC). Then the

effects of saponins were studied on the structure of human

serum albumin (HSA) by UV-Visible and FT-IR

spectroscopies. The results showed that Tribulus terrestris

saponins can disrupt the HSA structure and can interact

through the C=O and C-N groupsof the polypeptide chain.

These results provided valuable clues to the

pharmacokinetics and the pharmacologic activities of

Tribulus terrestrissaponins.

Keywords: Tribulus terrestris, Human Serum Albumin

(HSA), Saponins, Spectroscopy

Investigation of benzene biological effect on

hen egg white lysozyme structure and

function

Masoumeh Valipour1, Parvaneh Maghami2* 1 Department of Biolog, Faculty of Science, Azarbaijan Shahid Madani University, Tabriz, Iran 2 Department of Biology, Faculty of Science and Research, Islamic Azad

University, Tehran, Iran * Corresponding author: [email protected]

Benzene is an organic and aromatic chemical substance

which has the worst effect on biological systems among all

the pesticides. Too many deaths have been reported by

medical approaches that are caused by benzene. Industrial

activities are the main source of the benzene pollution. In

order to study the biological effects of benzene at the

molecular level, the interaction of lysozyme with benzene

was investigated. Lysozyme has an important role in non-

specific immunity and plays a critical role in defense

against microbial invasion. It has an obvious role in the

antimicrobial activity. As a carrier agent, lysozyme is

involved in antitumor processes. The interaction of

benzene with hen egg white lysozyme has been

investigated in this study. The change of protein structure

and function is determined by spectroscopy methods. In

order to understand the mechanism of benzene effect on

lysozyme, chemiluminescence method was performed.

Lysozyme absorbance was increased in the presence of

different concentrations of benzene. The extrinsic

fluorescence of protein was decreased by benzene

addition. Results show the increasing production of free

radicals according to increase in benzene concentration. It

can be concluded that the damaging effect of benzene on

lysozyme is through the production of reactive oxygen

radicals. So we can conclude that the mentioned structural

changes can induce functional alterations in protein.

Malfunctions of this protein affect different biological

processed at cellular levels.

Keywords: Benzene, Lysozyme, Chemiluminescence


44

Study of eye lens alpha crystalline structural

changes upon interaction with methyl tert-

butyl ether (MTBE)

Masoumeh Valipour1, Parvaneh Maghami2*, Aida Jalili Bolhasani2

1 Department of Biolog, Faculty of Science, Azarbaijan Shahid Madani

University, Tabriz, Iran 2 Department of Biology, Faculty of Science and Research, Islamic Azad University, Tehran, Iran


Methyl tert-butyl ether (MTBE) is an oxygenate additive

to increase the octane quality of gasoline and decrease air

CO content. MTBE can cause environmental pollution and

has the cancer-inducing potential as shown by different

researches on laboratory animals. Since MTBE is released

to air in large amount especially in the polluted air, it is

important to examine its interaction with proteins. In this

study, the effect of MTBE on eye lens alpha crystalline has

been investigated. In order to investigate this interaction,

different concentrations of MTBE were added to the

protein solution (from 5 to 35 µM). Different

spectroscopic methods such as UV- Vis, fluorescence, and

chemiluminescence have been applied to show the

structural alterations. The protein absorbance was

increased by increasing MTBE concentration. Alpha

crystalline extrinsic fluorescence emission was also

increased as a result of MTBE addition. Furthermore, the

amount of reactive oxygen species was increased in the

presence of MTBE. Results show that MTBE can induce

significant structural changes to alpha crystalline. This

may lead to consequent functional problems which cause

different eye illnesses.

Keywords: Alpha crystalline, MTBE, Spectroscopy

Investigation of the binding mechanism and

inhibitory effect of 2-hydroxy-1,4-

naphthoquinone on catalase activity and

structure: multi-spectroscopic and

computational study

Simin Khatayi, Gholamreza Dehghan*, Samaneh Rashtbari

Department of Biology, Faculty of Natural Sciences, University of Tabriz * Corresponding author: [email protected]

2-hydroxy-1,4-naphthoquinone; HNQ as a naphthoquinone

derivative, is the biologically active component of Henna

leaves. In this study, the effects of HNQ on the structure

and activity of bovine liver Catalase (BLC), has been

studied using various kind of spectroscopic and theoretical

methods including ultraviolet-visible (UV-vis) absorption,

fluorescence, ATR-FTIR spectroscopy, and molecular

docking studies. In vitro kinetic study showed that by

adding gradual concentrations of HNQ, catalase activity

was significantly decreased through noncompetitive

inhibition mechanism. UV–vis and ATR-FTIR

spectroscopic results illustrated that additional

concentration of HNQ leads to significant change in

secondary structure of the enzyme. The fluorescence

spectroscopic results at two different temperatures

demonstrated that HNQ can quench the intrinsic

fluorescence of BLC through a dynamic mechanism.

Changing the microenvironment around aromatic amino

acids (tryptophan (Trp) and tyrosine (Tyr)) resulted from

synchronous fluorescence. The thermodynamic parameters

were implied thatBLC has one binding site for HNQ the

hydrophobic interactions play an important role in the

formation of the HNQ-BLC complex by a spontaneous

process. Molecular docking data in agreement with

experimental results in this study confirmed that

hydrophobic interactions are dominant and there is only

one binding site in the middle of the β-barrel, helical

domain and wrapping domain of BLC for HNQ. Inhibitory

effect of HNQ on BLC activity indicated that in addition to

their beneficial impacts, they should not beoverlooked for

their side effects.

Keywords: Bovine liver catalase, 2-hydroxy-1,4-

naphthoquinone, Antioxidant

45

Inhibitory effect of Orange Yellow S on the

structure and the function of catalase:

Spectroscopic methods combined with

theoretical studies

Simin Khatayi, Gholamreza Dehghan*, Samaneh Rashtbari

Department of Biology, Faculty of Natural Sciences, University of Tabriz * Corresponding author: [email protected]

The study on the conformational and functional changes of

catalase as an antioxidant defense system of the liver, in

the presence of various compounds has been very

important. In the present study, the interaction between

Orange Yellow S and catalase were investigated using

multiple spectroscopic (fluorescence, ATR-FTIR, and

UV–vis) and molecular docking methods. Orange Yellow

S; OYS is a synthetic food additive that have been used

widely in food industry. Results of in vitro kinetics study

demonstrated that OYS leaded to a significant reduction in

the catalase activity through competitive inhibition

mechanism. Also, the fluorescence quenching data showed

a gradual decrease in the intrinsic fluorescence emission at

two different temperatures by a dynamic mechanism and

an alteration in the micro-region around aromatic amino

acids (tryptophan (Trp) and tyrosine (Tyr)).

Thermodynamic parameters were illustrated that OYS has

more than one binding site on BLC. UV–vis and ATR-

FTIR spectroscopic have been used for monitoring the

changes in secondary structure of the enzyme. Molecular

docking study results were in good agreement with

experimental data. For the assurance of consumer health, it

is important to control OYS in foodstuff. Because it may

lead to several serious health problems by affecting on

macromolecules.

Keywords: Bovine liver catalase, Orange Yellow S, Food

dyes

Stabilizing of catechol 1,2 dioxygenase on the

surface of magnetic nanoparticles and

investigating the enzyme activity

Nasrin Ahmadpour*, Soraya Mohammadzadeh, Vahab Jafarian, Bahram

Amini, Jamshid Mehrvand Department of Biology, Faculty of Science, University of Zanjan,

Zanjan, Iran

* Corresponding authors: [email protected]

Catechol 1,2 dioxygenase is one of the important enzymes

in the removal and analysis of phenolic compounds from

the environment. In the process of biodegradation, the non-

stabilized enzymes are inactivated as affected by

environmental cues. One of the strategies to cope with the

problem is to stabilize the enzyme. In the present study,

catechol 1, 2 dioxygenase was stabilized on the surface of

magnetic nanoparticles (MNPs). Firstly, the MNPs were

synthesized by tetraethoxysilane and 3-

aminopropyltriethoxysilane containing modified hydroxyl

and amine groups, respectively. To investigate the

structure and the size of synthesized MNPs,

ScanningElectron Microscope (SEM) and dynamical light

scattering (DLS) were used. The enzyme was then

stabilized on MNPs by EDC and NHS linkers at

concentrations of 5 μg/mL. The concentration of MNPs,

catechol 1, 2 dioxygenase, EDC and NHS linkers, the

reaction temperature, and the time required for

determining the enzyme activity were also optimized.

Finally, the enzyme activity was determined under

optimum conditions at 260 nm wavelength by

spectrophotometry. The results of the SEM and DLS

showed that the size of the synthesized MNPs was 23-35

nm. The results of comparing the activity of enzyme

showed that the stabilized enzyme had an approximately

1.5 times more than a non-stabilized enzyme.

KeyWords: Catechol 1, 2 dioxygenase, Enzyme

stabilization, MNPs

46

Investigation of activity the catechol 2, 3

dioxygenase immobilized on the surface of

silica nanoparticles

Soraya Mohammadzadeh*,Nasrin Ahmadpour, Vahab Jafarian, Bahram

Amini, Jamshid Mehrvand. Department of Biology, Faculty of Science, University of Zanjan, Zanjan,

Iran


Today, various pollutants such as metallic, organic,

phenolic and petroleum compounds are increasing in the

environment. Therefore, it is essential to serve as a

strategy for decomposing and decontaminating of these

compounds. One of the important methods of

detoxification is to use of microbial enzymes. The catechol

2, 3 dioxygenase is one of the important enzymes for the

decomposition of phenolic compounds. In the present

study, the results of expression and purification of the

catechol 2, 3 dioxygenase were determined by SDS-

PAGE. Silica nanoparticles were synthesized and their size

and shape were studied by scanning electron microscopy

(SEM) and dynamic light scattering (DLS). The enzyme

was stabilized on the surface of silica nanoparticles by

EDC and NHS linkers at concentrations of 5 μg mL-1

.

Then, the activity of both stabilized and the non-stabilized

enzyme was determined at 375 nm by spectrophotometry

and compared with each other. The results showed that the

size of the silica nanoparticles was 85-93 nm. The activity

of stabilized catechol 2, 3 dioxygenase was approximately

2 to 2.2 times more than that of the non-stabilized enzyme.

Keywords: Catechol 2, 3 dioxygenase, Enzyme

immobilization, Silica nanoparticles

Investigating the activity of

cyclomaltodextrinase stabilized on the surface

of superparamagnetic iron oxide

nanoparticles with modified core-shell

Jamshid Mehrvand1*, Vahab Jafarian2, Bahram Amini2, Khosrow

Khalifeh2, Nasim Hayati Roodbari1, Leila Hasani3, Nasrin Ahmadpour2, Souraia Mohammadzadeh2

1 Department of Biology, Science and Research branch, Islamic Azad

University (IAU), Tehran, Iran 2 Department of Biology, Faculty of Science, University of Zanjan,

Zanjan, Iran 3 Department of Biological Science, Institute in Advanced Studies in

Basic Sciences (IASBS), Zanjan, Iran


The cyclomaltodextrinase (CDase) belongs to the enzymes

of the glycohydrolase family that participates in the starch

degradation process. The enzyme specifically decomposes

cyclomaltodextrin (CD) into maltose and glucose. In the

present study, CDase was stabilized on the surface of

superparamagneticiron oxide nanoparticles (SPIONs).

Firstly, the SPIONs were synthesized in the presence of

ammonia in a sedimentary method. The surface of the

nanoparticles was coated using tetraethoxysilane (TEOS)

and 3-Aminopropyltriethoxysilane(APTES) containing

hydroxyl and amine functional groups, respectively.

Finally, the enzyme was coupled to the SPIONs using 5 μg

and 2 μg EDC and NHS, respectively. The activity of both

stabilized and the non-stabilized enzyme was measured

and compared with each other by the DNS method. The

structure of synthesized SPIONs was also studied by

scanning electron microscope (SEM). According to the

SEM results, the shape of the synthesized SPIONs was

spherically and they were 35 nm in size. Our results also

showed that the efficiency of conjugation enzyme on the

SPIONs using Bradford method was 98%, demonstrating

that the stability of the immobilized enzyme increased in

comparison with the free enzyme. The results of such

studies could improve CDase properties enabling it to be

applicable in a wide range of temperature, and after a long-

term storage.

Keywords: Cyclomaltodextrinase, Enzyme stabilization,

Superparamagnetic Iron Oxide Nanoparticles (SPIONs)

47

Detection of H2O2 and glucose using

peroxidase mimicking activity of CuO/GFs

aerogel

Navvabeh Salarizadeh1, Shirin Shahangian2, Ali Foroutan2, Reza Hassan

Sajedi3 1 Department of Biochemistry & Biophysics, Education and Research

Center of Science and Biotechnology, Malek Ashtar University of

Technology, Iran 2 Department of Biology, Faculty of Sciences, University of Guilan, 3 Department of Biochemistry, Faculty of Biological Sciences, Tarbiat

Modares University * Corresponding author: [email protected]

In this research, CuO nanoparticles decorated on graphene-

based frameworks (CuO/GFs) were prepared by

hydrothermal method. The structure, composition, and

morphology of the prepared nanocomposite were

characterized using X‐ray diffraction and scanning

electron microscopy with energy dispersive X‐ray

spectroscopy, respectively. A colorimetric system

involving nanozyme, 3,3´,5,5´-tetramethylbenzidine

(TMB) and H2O2 was utilized for the determination of

peroxidase-like catalytic assay. Also, The Michaelis–

Menten constants (Km) and maximum initial velocities

(Vmax) of CuO/GFs was calculated using Lineweaver–

Burk plot. The obtained results confirmed that the

synthesis of CuO/GFs nanostructures was successful. It

was found that CuO/GFs nanohybrid exhibited peroxidase

mimetic activity. Peroxidase - like activity was highest at a

temperature of 30° Can dp H3.0 but was moderately active

at a temperature of 20-60°C. Also, a combination of the

peroxidase‐like catalytic activity of the CuO/GFs

nanohybrid with glucose oxidase (GOX) allowed the

system of a sensitive colorimetric assay for glucose

monitoring.

Keywords: CuO/GFs aerogel, Peroxidase-like activity,

Characterization, Tetramethylbenzidine

Effect of 3-beta-hydroxybutyrate on the

formation of human serum albumin amyloid

fibrils

Hojat Mohammadnia1, Mansour Ghaffari Moghaddam1, Neda Poormolaie1, Mousa Bohlooli2*

1 Department of Chemistry, Faculty of Science, University of Zabol, Iran 2 Department of Biology, Faculty of Science, University of Zabol, Iran * Corresponding author: [email protected]

The overall flexibility of the protein influences on its

biological function.Changing the structure of the normal

protein prone to aggregation.Also, factors such as amino

acid sequencing, mutation and environmental stresses, pH,

temperature, anxiety, chemical species and oxidative

agents lead to disruption of the protein structure,

denaturation, aggregation, and consequently protein

dysfunction. Glycation is one of the factors which results

in a change in the protein structure and function and the

production of the fibril. Formation of accumulated protein

forms, especially fibrils and their toxic intermediates is the

main cause of some diseases, such as Alzheimer's,

Huntington's and Parkinson's diseases. In this study, the

human serum albumin fibrillation process in the presence

of 3-beta-hydroxybutyrate was studied in diabetic

conditions. For this purpose, human serum albumin (HSA)

with glucose and 3-beta-hydroxybutyrate was treated for a

long time under physiological conditions. To evaluate the

effect of 3-beta-hydroxybutyrate, circular dichroism

spectroscopy, UV-Vis spectroscopy, fluorescence

spectroscopy and atomic force microscopy were used. The

results show that the structural changes of HSA and the

sugar-protein proximity products decreased in the presence

of 3-beta-hydroxybutyrate. Therefore, based on this study,

3-beta-hydroxybutyrate ketone body can play an inhibitory

effect on fibrillation of human serum albumin protein and

the disease control.

Keywords: Fibril, 3-beta-hydroxybutyrate, Ketone body,

Glycated human serum albumin



48

Investigation of the inhibitory effect of two

new phenol-ninhydrin derivatives on

humansalivary α-amylase enzyme

Narges Alipour Saqa1, Shiva Khalil- Moghaddam2*, Ashraf Shahvelayati2

1 Department of Biology, Yadegar-e-Imam Khomeini (RAH) Shahre Rey Branch, Islamic Azad University, Tehran, Iran 2 Young Researchers and Elite Club, Yadegar- e- Imam Khomeini (RAH)

shahr-e-Rey Branch, Islamic Azad University,Tehran, Iran * Corresponding author: [email protected]

Inhibition of α-amylase, an enzyme that plays a role in the

digestion of starch and glycogen, is considered asa strategy

for the treatment of disorders in carbohydrate uptakes,

such as diabetes and obesity. Inhibitors of carbohydrate-

digesting enzymes, such as alpha-amylase and alpha-

glucosidase, are now actively searched for since they could

ultimately make useful medicines against diabetes and

obesity. In the present study, by considering the structural

requirements, two new phenol- ninhydrin derivatives as α-

amylase inhibitor were synthesized. Inhere, pyrogallol has

been used as a polyphenol compound. Ninhydrin-

Pyrogallolmonoadduct andNinhydrin-Pyrogallolbisadduct

derivativescharacterized by spectral analysis and finally

evaluated for the inhibition of human salivary α-amylase

activity by the method of Bernfeld. Mono and bis adduct

derivatives exhibited their best α-amylase inhibitory

activity at a 1 millimolar concentration (23and 69.4

Percentageof inhibition). Results showed that bis adduct

derivative can be explored as a strong anti-hyperglycemic

agent. Putative binding mode of two derivatives with the

target enzyme was also explored by the docking studies.

Keywords: Diabetes, Human salivary alpha-amylase,

Ninhydrin pyrogallol derivatives

Lipase immobilization on aluminum-based

periodic mesoporous organosilica (PMO)

support as a biocatalyst for biodiesel

production

Soroush Soltani1, Parisa Fathi Rezaei1, Esmail Doustkhah2*, Yunske Ide2,

Behrouz Nasseri1, Hamid Taghavi Dehaghani1 1 Department of Biology, Faculty of Basic Sciences, University of

Maragheh, Iran 2 International Center for Materials Nanoarchitechtonics (MANA), Tsukuba, Japan


Nowadays, a tendency towards immobilization of enzyme

has been increased due to its advantages including

retaining activity after each cycle, easy recovery and

raising the stability. Furthermore, concerns around the use

of fossil fuels and emission of toxic gases into the

atmosphere have made a crisis for human being and

environment. Diminishing fossil fuel resources,

environmental hazards associated with fossil fuel

combustion emissions and the increasing necessity for

energy uses are the main reasons for researches to replace

it with alternative fuels. Biodiesel production has attracted

a considerable interest as sustainable fuels because of its

low sulfur content, low hydrocarbon aroma, high octane

number, high flash point, and low environmental impact.

Biodiesel is a mixture of long-chain fatty acid methyl

esters (FAME) produced from the transesterification of

triglycerides. Here, porcine pancreatic lipase (PPL) was

selected to immobilize inside the pore channels of

aluminum-based PMO. Enzyme immobilization was

confirmed by scanning electron microscopy (SEM),

Transmission electron microscopy (TEM), low angled

XRD, FTIR, EDAX, and BET. Lipase activity was

determined spectrophotometrically using p-NPP as the

substrate. One unit of lipase activity was defined as the

amount of enzyme required to release 1 μmol of p-

nitrophenol per milliliter per minute. Protein content was

monitored by the Bradford method. In a normal laboratory

condition, adding substrate (Triglyceride) to immobilized

enzyme was done to testify the activity of the immobilized

lipase in transesterification. All the analyses were in

agreement with our predictions. We successfully reached

the results where the biodiesel was produced very

efficiently.

Keywords: Enzyme immobilization, Biodiesel, Fuel

49

The study of protective effects of Propolis

against X radiation on MCF-7 cell line

Hamid Azizi1, Ayub Karimzadeh2,3 *, Saber Zahri1 1 Department of Cell & Molecular Biology, University of Mohaghegh Ardabili, Ardabil, Iran 2 Department of Biochemistry, Higher Education Institute of Rab-Rashid,

Tabriz, Iran

3 Drug Applied Research Center, Tabriz University of Medical Sciences,

Tabriz, Iran


Breast cancer is a major health problem for women all

over the world. It is the second most common cancer after

the lung cancer and the most common cause of cancer

death among women. Propolis contains flavonoids and

polyphenols that have notable antioxidant and anti-cancer

and anti-microbial effects. Propolis samples were extracted

with 96% ethanol. MCF-7 cells were cultured in RPMI

medium. EEP extract was dissolved in DMSO and used for

cell treatment. The effect of Propolis on the viability of

MCF-7 cells was determined by MTT assay. Effects of X-

radiation along with extracts by assessment of the

viability, cell apoptosis, and molecular methods were

determined. The results showed that Propolis extracts in

low concentrations had not significant lethal effect. As

well as X-ray treatment had not lethal effect at the dose of

1 Gy, while the X-ray treatment with extracts showed

significant cytotoxic effect. Ic50 of X-radiation and extract

treatments was 14 micrograms/ml. In this study, AO/EB

and Annexin-V/PI tests were used in order to search for

apoptosis in the treated cells. The rate of apoptosis in cells

treated by X-ray along with 15 micrograms/ml of extract

was significantly increased in compared with the control.

These findings suggested that Propolis had not strong

cytotoxicity effects in the low concentrations. However,

the extract treatments along with the other compounds (X-

ray) were significantly cytotoxic.

Keywords: Apoptosis, X-ray, Propolis, MCF-7, MTT

Study of the interaction between indinavir

and complex (DNA-H1) by multispectroscopic

techniques

Mozhgan Mohammad Hosseinzadeh Noghondari1, Mohammad Reza

Saberi2, Jamshid Khan Chamani3* 1 Department of Biochemistry and Biophysics, Faculty of Sciences,

Mashhad Branch, Islamic Azad University, Mashhad, Iran 2 Department of Medicinal Chemistry, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran 3 Department of Biochemistry and Biophysics, Faculty of Sciences,

Mashhad Branch, Islamic Azad University, Mashhad, Iran * Corresponding author: [email protected]

The interaction of small molecules with DNA plays an

important role in many biological processes .DNA binding

studies have remarkable relevance and implications in

drug design aspects and cancer chemotherapy. Histones act

as spools around which DNA winds. This enables the

compaction necessary to fit the large genomes of

eukaryotes inside cell nuclei .Histones undergo

posttranslational modifications that alter their interaction

with DNA and nuclear proteins. Indinavir group of HIV

drugs called protease inhibitors. The enzyme in HIV called

protease is blocked by PI. Pls, prevent HIV from

multiplying and can reduce the amount of HIV in the

body. The chemical formula of Indinavir is

CH36H47N504, and its molecular weight is 613.79. The

interaction between complex (DNA-H1) and indinavir has

been studied by using different spectroscopic techniques

vis., resonance light scattering (RLS) spectra and circular

dichroism (CD) spectra, in physiological buffer pH=6.8.

The RLS method determined the critical aggregation

concentration of drug on complex (DNA-H1). When

indinavir was added to (DNA-H1) complex, the RLS

intensity of the system was increased. It is known that RLS

enhancement could be the result of the enlargement of the

molecular volume and large size of the complex Circular

dichroism is a suitable method for monitoring the

secondary and tertiary structure of proteins. Quantitative

analyses of the CD spectra of the interaction between

(DNA-H1) and drugs secondary structure. Change of the

secondary structure of DNA-H1 involving an increased of

α-helix, and chirality has increased Electrostatic forces and

hydrogen bonds.

Keywords: Indinavir, Complex-DNA-H1, RLS, CD



50

Study of the interaction between Nelfinavir

and complex (DNA-H1) bymultispectroscopic

techniques

Mozhgan Mohammad Hosseinzadeh Noghondari1, Mohammad Reza

Saberi2, Jamshid Khan Chamani* 1 Department of Biochemistry and Biophysics, Faculty of Sciences,

Mashhad Branch, Islamic Azad University, Mashhad, Iran 2 Department of Medicinal Chemistry, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran 3 Department of Biochemistry and Biophysics, Faculty of Sciences,

Mashhad Branch, Islamic Azad University, Iran * Corresponding author:[email protected]

The interaction of small molecules with DNA plays an

important role in many biological processes .DNA binding

studies have remarkable relevance and implications in

drug design aspects and cancer chemotherapy. Histones act

as spools around which DNA winds. This enables the

compaction necessary to fit the large genomes of

eukaryotes inside cell nuclei .Histones undergo

posttranslational modifications that alter their interaction

with DNA and nuclear proteins. Nelfinavir (brand name

Viracept) is an antiretroviral drug used in the treatment of

the human immunodeficiency virus (HIV). Nelfinavir

belongs to the class of drugs known as protease inhibitors

(PIs) and like other PIs is almost always used in

combination with other antiretroviral drugs .Its chemical

formula is CH32H45N5O4S and its molecular weight is

568.784 g / mol. The interaction between complex (DNA-

H1) and Nelfinavir it has been studied by using different

spectroscopic techniques vis., resonance light scattering

(RLS) spectra and circular dichroism (CD) spectra, in

physiological buffer pH=6.8. The RLS method determined

the critical aggregation concentration of drug on complex

(DNA-H1). When nelfinavir was added to (DNA-H1)

complex, the RLS intensity of the system was increased. It

is known that RLS enhancement could be the result of the

enlargement of the molecular volume and large size of the

complex Circular dichroism is a suitable method for

monitoring the secondary and tertiary structure of proteins.

Quantitative analyses of the CD spectra of the interaction

between (DNA-H1) and drugs secondary structure.

Change of the secondary structure of DNA-H1 involving

an increase of α-helix and chirality has increased

Electrostatic forces and hydrogen bonds.

Keywords: Nelfinavir, Complex-DNA-H1, RLS, CD

Changes of serum level of prolactin following

the X-ray radiation

Fereshteh Dadfar, Kourosh Bamdad, Zahra Mortazavi

Departement of Biology, Faculty of Sciences, Payame Noor University, Iran


Today, many diseases are treatable with x-rays. On the

other hand, the rays have harmful effects on human health.

Regarding the destructive effects of X-rays on the activity

of endocrine glands and possible dysfunction of the

hormone caused by their functional impairment, the aim of

this study was to investigate changes in serum levels of

prolactin following X-rays. 40 male rats were at a

weighing range of 250 to 300 g selected randomly. The

first group (n=20) was considered as the control group and

the second group (n= 20) was exposed to the X-ray once.

After the anesthesia of the mice, 2cc of blood were taken

from the heart of all of them for hormonal studies. The

ELISA method was used to measure serum levels of

prolactin. T-Test analysis was used to compare the mean

of prolactin level in experimental groups. Data analysis did

not show the significant difference in prolactin levels in

the X-ray group (19.21±0.76) with compared to the control

group (18.98±0.68), (P>0.05). Therefore, it can be

concluded that radiation therapy with X-rays does not have

any significant effect on some of the glands and their

related hormonal changes.

Keywords: Prolactin, X-ray radiation, Serum changes



51

Studies on the interaction of the drug

Indinavir with calf thymus DNA by resonance

light scattering and circular dichroism

spectroscopy

Ladan Shakoorzadeh Ardebili1*, Mohammad Reza Saberi2, Jamshid Khan

Chamani1 1 Department of Biochemistry and Biophysics, Faculty of Sciences,

University of Islamic Azad, Mashhad Branch, Iran 2 Department of Medicinal chemistry, Faculty of Pharmacie, University of Ferdosi, Mashhad Branch, Iran


The molecular DNA contains all the information necessary

for theDevelopment and biological functioning of the

living organisms and the virus.DNA is one of the

biological macromolecules that plays a role in the

transmission of genetic information. This macromolecule

is a long polymer of simple building blocks called

nucleotides And according to Watson & Creek Model,

DNA is Right-handed double-helix with a major and a

minor grooveSo that The organic bases are located inside

the helix and the sugar-phosphate “backbone” on the

outside and connection of the sugar and the phosphate

groups is carried out through the ester bond.Bases that are

opposite each other are paired according to defined rules

as a result of hydrogen bonds. Indinavir is a viral protease

inhibitor used to treat HIV and results in the release of

abnormal and non-infectious viruses. In this study, the

interaction between Indinavir drug and calf thymus DNA

at ambient temperature and pH=6.8 was investigated by

resonance light scattering and circular dichroism

spectroscopy. According to the results arise from

resonance light scattering we conclude that a change in the

macromolecular structure, which confirms the formation

of a complex between the Indinavir and the calf thymus

DNA. Thechart Critical Point of Creation ofSediment in

the presence of increasing amounts of indinavir, in

addition to the emphasis on the formation of a complex

between drug and DNA, was used to determine the critical

concentration of sediment generation. Cd spectroscopy

was used to study the structural changes in DNA in

interaction with Indinavir the results of the cd spectra

showed that the interaction of Indinavir with DNA caused

a change in the structure of the second DNA. Also,

according to the results obtained, it is likely that this

interaction occurs between the base pair of DNA.

Keywords: Indinavir, DNA, Resonance light scattering,

Circular dichroism

Studies on the interaction of the drug

Nelfinavir with calf thymus DNA by

resonance light scattering and circular

dichroism spectroscopy

Ladan Shakoorzadeh Ardebili1*, Mohammad Reza Saberi2, Jamshid Khan

Chamani1 1 Department of Biochemistry and Biophysics, Faculty of Sciences,

University of Islamic Azad, Mashhad Branch, Iran 2 Department of Medicinal Chemistry, Faculty of Pharmacie, University of Ferdosi, Mashhad Branch, Iran


The DNA molecule carries all the information necessary to

form the physical properties of the individual, and these

properties are essentially determined by the protein.

Therefore, DNA contains instructions for making a

protein. In fact, DNA has included in its structure the

information that inherits the living entities of its

predecessors. A slight change in the structure of DNA may

have serious consequences and, if it is degraded to an

irreversible extent, causes the death of the cell. Nelfinavir

is an antiretroviral agent that, as an HIV protease inhibitor,

can inhibit both type 1 and type 2 HIV protease. Usually,

this drug is used to treat HIV infection with a reverse

transcriptase inhibitor. In this study, the interaction

between Nelfinavir drug and calf thymus DNA at ambient

temperature and pH= 6.8 was investigated by resonance

light scattering and circular dichroism spectroscopy. The

resonance light scattering results showed a change in the

macromolecule structure, indicating the formation of a

complex between the nelfinavir and the calf thymus DNA.

The chart Critical Point of Creation Sediment on the

nelfinavir concentrations emphasized the formation of the

complex between the drug and the DNA. Further, it

showed that the drug in the lower concentrations could

also cause changes in the DNA structure. cd spectroscopy

was used to investigate the structural changes in DNA in

interaction with nelfinavir. Spectrometry results show that

the interaction of nelfinavir with DNA leads to a change in

the structure of the second macromolecule. Also,

according to the results obtained, it is likely that this

interaction occurs between the pair portions of DNA.

Keywords: Nelfinavir, DNA, Resonance light scattering,

Circular dichroism


52

The effect of different coating surface on

Silver nanoparticles interaction with human

serum album (HSA)

Zahra Roshani1, Azadeh Hekmat1*, Mohammad Yousefi2

1 Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran

2 Department of Chemistry, Science and Research Branch, Islamic Azad

University, Tehran, Iran * Corresponding author: [email protected],[email protected]

Among the inorganic nanoparticles, silver nanoparticles

(Ag NPs) has been utilized most widely in the

nanotechnology and medical industry due to its anti-

infections and anti-tumor activities. The aim of this study

is to evaluate the effects of Polyethylene glycol (PEG) and

Sodium citrate coating on Ag NPs interaction with human

serum albumin (HSA) with Fluorescence spectroscopy,

Fourier-transform infrared spectroscopy (FTIR) and Zeta

potential studies. The Fluorescence data and binding

constants ‎dewohsthat PEG-coated Ag NPs could interact

with HSA more strongly and make structural changes

compare with Sodium citrate-coated Ag NPs. FTIR studies

and Zeta-potential analysis also confirmed the structural

changes of HSA after interaction with PEG-coated Ag NPs

strongly. The results obtained from this study proffer a

good strategy for designing new drugs and standardization

in nanotechnology.

Keywords: Human serum albumin (HSA), PEG-coated-

silver nanoparticles, Sodium citrate coated- silver

nanoparticles

The structural changes of hormone Human

Chorionic Gonadotropin (hCG) in Ultrasound

exposure

Atieh Gheisari1, Azadeh Hekmat1*, Adeleh Divsalar2 1 Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran 2 Department of Cell and Molecular Biology, Faculty of Biological

Sciences, Kharazmi University, Tehran, Iran * Corresponding author: [email protected] ,[email protected]

The hormone human chorionic gonadotropin (better

known as hCG) is a glycoprotein produced during

pregnancy. It is made by cells formed in the

placenta.Identifying the various effects of noise pollution

on physiological characteristics of living organisms in

order to help reduce its effects must be taken seriously. So,

in this research, the structural changes of hCG in

ultrasound exposure was studied with UV-Visible

spectroscopy, Fluorescence spectroscopy and Fourier-

transform infrared spectroscopy (FTIR). The hyperchromic

in UV-Vis and a clear increase in the Trp fluorescence

suggest that the protein structure changed after 30 min

ultrasound exposureand unfolded. Furthermore, the FTIR

studies evaluated the changes in bending and stretching

bonds of functional groups of the hormone. Furthermore,

the secondary structure of hCG changed and a transition

from α-helix to β-structure after 30 min

ultrasoundappeared. According to the findings of this

study, it seems that sound pollution threats the health of

pregnant women and fetus. Consequently, it is

recommended to present necessary training about the

hazardous effects of sound pollution on the pregnancy of

women residing in crowded areas of the city and also to

adopt ways to diminish this pollution.

Keywords: Human Chorionic Gonadotropin (hCG),

Ultrasound, UV-Visible spectroscopy, Fluorescence

spectroscopy, Fourier-transform infrared spectroscopy

(FTIR)

53

Effect of ultraviolet radiation on total phenol

in Thymus vulgaris L.

Mojtaba Norouzi*1, Forough Sanjarian1, Samira Shahbazy2

1 Department of Plant Bio-Product, Institute of Agricultural Biotechnology, National Institute of Genetics and Biotechnology, Iran

2 Nuclear Agriculture Research School, Nuclear Science and Technology

Research Institute (NSTRI), Atomic Energy Organization of Iran (AEOI) * Corresponding author: [email protected]

Thyme (Thymus vulgaris L.) is a medicinal plant

belonging to the Lamiaceae family, which has valuable

and useful compounds used in the pharmaceutical and

sanitary industry. Nowadays, the study of the essential oil,

anti-spasmodic, antioxidant and antimicrobial properties

(anti-bacterial and anti-fungal) have been proven. The

analysis of thyme essential oil by GC / MS method showed

a high concentration of phenolic compounds than other

compounds. This indicates the importance of these

compounds on the medicinal properties of the plant. In this

study, the effect of ultraviolet radiation on the

concentration of phenolic compounds of thyme was

investigated. The dried aerial parts of the plant were

exposed to radiation UV-B (280-315nm) for 18 hours.

Then, the total phenol content was measured at 20,100 and

150μl concentration in 3 replicates by the Folin-ciocalteau

colorimetric method. Statistical analysis was performed

between the data.Total phenol content in UV treatment

(mean 205.28) was significantly higher than control (mean

195.45).

Keywords: Thymus vulgaris L., Total phenol, Ultraviolet

radiation

Magnetic nanocomplex design for

transferring cisplatin

Farank Mohammad Ashoori, Mohammad Sattari, Parviz Abdolmalaki

Tarbiat Modares University, Faculty of Life Sciences, Department of Biophysics


The abundant use of platinum drugs, including cisplatin,

has been associated with the development of cellular

resistance in the treatment of various cancers. The

resilience of the cells leads to reduced efficacy, increased

dosage of the drug, and ultimately increased side effects of

the drug on normal cells and tissues. The purpose of this

study is to transfer the drug using a magnetic nanocomplex

to increase the effectiveness of the drug and subsequently

reduce the dose of the drug. This nanocomplex was formed

by attaching magnetic nanoparticles of iron oxide to a

polyethylene amine cationic polymer and eventually

adding the drug to it. The accuracy of the formation of the

nanocomplex (magnetic nanoparticles-polyethyleneimine-

cisplatin) was evaluated using FTIR and DLS tests.

Finally, the cell death rate induced by this nanocomplex

compared to the single drug (at the same concentration) on

A2780 sensitive and resistant to cisplatin cells was

investigated by MTT technique and IC50 of each treatment

group was calculated. The results of the FTIR and DLS

showed that nanocomplex magnetic is formed and its size

is suitable for transmission. The results from the MTT test

showed that the nanocomplex significantly induced cell

death in the drug. Studies have shown that drug delivery

with this nanocomplex regulates drug release in the cell,

resulting in increased drug exposure and reduced

resistance.

Keywords: Platinum drugs, Nanocomplex, Cisplatin



54

Purposeful transfer of polyethyleneimine

polymer using magnetic nanoparticles and

static magnetic fields to normal and cancerous

cells

Farank Mohammad Ashoori, Mohammad Sattari, Parviz Abdolmalaki*

Tarbiat Modares University, Faculty of Life Sciences, Department of Biophysics


Magnetic nanoparticles have the ability to function at

cellular and molecular levels due to their properties,

including magnetic properties, which has made them an

attractive vector for delivery of medication. Cationic

polymers, such as polyethyleneimine, are positive-

polymeric polymers that are active in active groups and

have therefore been used extensively in biology, and their

most important application is gene transfer. The main

problem with these polymers is their biodegradability,

which induces cell toxicity. Studies show that

polyethyleneimine polymer, by binding to negative

pregnant proteins within the cell, leads to induction of

apoptosis. The purpose of this study was to transfer

polyethylene immune polymer as a drug using magnetic

nanoparticles for induction of cell death of cancer cells. To

this end, the binary polyethylene amine-magnetic

nanoparticle complex (MNP-PEI) was formed by attaching

magnetic nanoparticles of iron oxide synthesized to the

cationic polymer and confirmed by FTIR test. In the

following, normal fibroblast cells and breast cancer with

different concentrations of binary complex and polymer

were treated only in presence and absence of a static

magnetic field. The rate of cell death in different treatment

groups was calculated using the MTT test. IC50 of

different groups was calculated. Results showed that in the

presence of a magnetic field of binary complex (MNP-

PEI), compared to polymer only, the percentage of cell

survival significantly decreased.

Keywords: Magnetic nanoparticle complex,

Polyethyleneimine, Gene transfer

The effect of electromagnetic pulses on the

glutamate-aspartate transporter (GLAST)

and glutamine synthase (GS) in the

hippocampus of male rats

Mohammad Ali Mohammadalizadeh, Kataneh Abrari*, Taghi

Lashkarblouki, Mohammad Taghi Ghorbanian, Departement of Biology, University of Damghan, Iran


The imbalance of inhibitory and excitatory

neurotransmitters in the nervous system causes many brain

disorders. Glutamate is the most important excitatory

neurotransmitter in the synapses which absorbed by

glutamate-aspartate transporter (GLAST) in astrocytes

membrane, then it has been converted to glutamine by

glutamine synthetize enzyme (GS) Within the cytoplasm

and then enters to vesicles.The purpose of this study was

to investigate the effect of electromagnetic pulses on the

expression of GLAST and GS genes and also GS activity,

in the hippocampus.Twenty male Wistar rats were

randomly divided into control and treatment groups.

Treatment group was submitted to daily pulsed

electromagnetic field (7 mT, 30 Hz for 16 min daily/ 14

days). Control group submitted to off electromagnetic

apparatus. At the end of the experiment, the hippocampus

of the mice was extracted. GS activity was measured and

GLAST and GS gene expression was evaluated using real

time PCR.The level of GS activity in the treatment group

was significantly higher than the control group (P≤0.05).

However, there was no significant difference in GS gene

expression in these two groups. The level of GLAST gene

expression did not show any significant difference

between the two groups. The 14-day treatment with 30 Hz,

7mT electromagnetic pulses, without affecting the GS

gene expression, resulted in increased activity of the

glutamine synthase astrocyte enzyme. The treatment was

not effective in expressing the carriers of glutamate-

aspartate astrocytes.

Keywords: Electromagnetic pulse, Astrocyte, Glutamine

Synthetize, Glutamate-Aspartate transporter



55

The magnetic Co1-xZnxFe2O4 nanostructure

interaction with DNA molecule study by

multiple spectroscopies

Samaneh Montazery1, Azadeh Hekmat1*, Adeleh Divsalar2, Alireza

Iranbakhsh1 1 Department of Biology, Science and Research Branch, Islamic Azad University,

Tehran, Iran 2 Department of Cell and Molecular Biology, Faculty of Biological Sciences,

Kharazmi University, Tehran, Iran

* Corresponding author: [email protected] ,[email protected]

Over the past few years, magnetic nanoparticles (NPs)

have become more and more significant for applications in

biomedicine and biotechnology. Cobalt ferrites NPs are

appropriate for the isolation and purification of genomic

DNA and particularly in hyperthermia treatment. In this

research, we have studied the interaction between

synthesized Co1-xZnxFe2O4nanostructure and DNA

molecule using UV-Visible spectroscopy, Fluorescence

spectroscopy and Fourier-transform infrared spectroscopy

(FTIR) at 37 ˚C. The UV-Visible spectroscopy results

suggested that after adding different concentrations of the

Co1-xZnxFe2O4 nanostructure to the DNA solution, the

DNA structure changed. Furthermore, the fluorescence

studies revealed the strong interaction between DNA and

Co1-xZnxFe2O4 nanostructure occurred. The FTIR

studies displayed the variations in bending and stretching

bonds of functional groups of DNAdue to the interaction

with Co1-xZnxFe2O4. The results obtaining from this

present study probably provide useful information to

design more efficiency magnetic anti-cancer drugs in the

future.

Keywords: DNA, Cobalt–Zinc Ferrite Nanostructure,

Spectroscopy, Magnetic nanostructure

Using a genetic algorithm to find promoter

and motif

Haleh Homayoni, Mohammad Zarei*

Department computer, Faculty of Higher Education, University of Apadana Shiraz, Iran


Identifying transcription factor binding sites in genes is an

important problem in biology. To predict the binding site

locations efficiently, many algorithms have been

developed. However, the prediction accuracy is not

satisfactory and binding site prediction thus remains a

challenging problem. In this promotional article,

we introduce develop an approach that can be used to

predict binding site promoters using a genetic algorithm.

Based on the generic framework of a genetic algorithm,

the approach explores the search space of all possible

starting locations of the binding site promoters and motifs

in different target sequences with a population that

undergoes evolution. Individuals in the population

compete to participate in the crossovers and mutations

occur with a certain probability. Initial experiments

demonstrated that our approach could achieve high

prediction accuracy in a small amount of computation

time. A promising advantage of our approach is the fact

that the computation time does not explicitly depend on

the length of target sequences and hence may not increase

significantly when the target sequences become very long.

To avoid the exhaustive search, many computational tools

have been developed to identify the common binding sites

of homologous genes based on the stochastic Gibbs

sampling algorithm that we used in this paper.

Keywords: Evulution algorithm, Genetic Algorithm,

Promoter, Genome


56

The effects of L-dopa on hypothalamic NPY

gene expressions in PCOS model rats

Leila Nezhaddadghar, Fariba Mahmoudi*, Saber Zahri, Alireza Panahi

Department of Biology, Faculty of Sciences, University of Mohaghegh Ardabili, Iran


Luteinizing hormone (LH) levels are high in polycystic

ovary syndrome(PCOS). Neuropeptide Y(NPY) and

Dopaminergic neurons activity are low in this patient.

Dopamine increases NPY release. In the present study, the

effects of L-dopa and dopamine receptor antagonists were

investigated on relative NPY gene expressions in the

hypothalamus of PCOS model rats. PCOS condition was

induced in fifteen Wistar female rats weighing 180-220g

by intramuscular injections of estradiol valerate. The

PCOS rats in three groups received saline, L-dopa

(100mg/kg), simultaneous injections of Sulpiride

(10mg/kg), SCH23390 hydrochloride (1mg/kg) and L-

dopa (100mg/kg) via intraperitoneal injection respectively.

Five intact estrous rats as a control group received saline

intraperitoneally. Hypothalamic samples were dissected.

Mean relativeNPY gene expressions were determinedby

real-time polymerase chain reaction (RT-PCR) method.

Hypothalamic NPY gene expressions significantly

increased in PCOS ratsin comparison to intact ones.L-dopa

significantly decreases hypothalamic NPY gene

expressions in comparison to PCOS rats. Injections of L-

dopa following Sulpiride and SCH23390 did not

significantly increase the NPY gene expressions compared

to L-dopa group. Increasing the Dopaminergic neurons

activity may be useful in the understanding of the

infertility mechanism of PCOS via regulating the synthesis

of neuropeptides of its neuroendocrine axis.

Keywords: Sulpiride, NPY, L-dopa, SCH23390

Ancient DNA extraction, identification,

molecular cloning and enzymatic

enhancement

Parastoo Erfanmanesh*, Kamran Ahmadi

Research Institute of Cultural Heritage and Tourism, Iran * Corresponding author: [email protected]

In the field of scientific research, it is anticipated that the twenty-

first century, contrary to the twentieth century, was dominated by

physics, a century of biology. Genetic information derived from

the DNA of human bones can help to study the relationships

existing between ethnic groups and in smaller dimensions of

groups and individuals buried in a cemetery, and can be useful in

determining the sex of skeletons. In the study of paleopathology

on bone samples, it is possible to identify inherited diseases and

infectious diseases that have contaminated the DNA in the bones.

The DNA obtained from archeological and paleontological

remains allows for the timely return and study of the genetic

linkages of extinct organisms to their contemporary family. This

poses a new perspective on the evolution of organisms and their

DNA sequencing. However, there are many technical problems

in this field and they require precise criteria to ensure the

credibility of the findings, especially in the study of human

remains. In recent years, the study of aDNA has expanded and

attracted many enthusiasts. The study of ancient DNA helps to

clarify the historical events from a more comprehensive

viewpoint and can be used in various areas of research and

research. At present, it is necessary to use biotechnology in the

development of cultural heritage, because its use in

understanding the culture of the Iranian ethnic groups can reveal

some dark points in Iranian history. One of its major

achievements is the acquisition of necessary information for the

development and analysis of genetic changes that have been

made among Iranian ethnic groups during the history of

immigration, wars, and so on. It should be noted that studies in

the field of ancientgenetics require specialized laboratories with

their own standards. The method used in ancient DNA studies is

that; the specimen is taken from the tooth structure, femur,

pterosa, tibia and humerus, and genetic studies are done in the D-

loop region of the mitochondria section. The sample conditions

are performed according to the skeleton conditions obtained from

the ancient area of the mentioned sections. In addition, the person

responsible for this should be prominent in its entirety, in order

not to create an error during the operation. The conditions of

work are carried out in completely specialized laboratories. To

remove any bone marrow, separate gloves should be used to

prevent any transmission of contamination from person to

sample. Also, the environmental conditions of the laboratory are

such that no other genetic and molecular studies in this

environment are accepted. The cultural heritage research

institute, as the representative of the Institute for the

Conservation and Restoration of Historical-Cultural Works, has

taken steps in this field over the past two years with the

assistance of reputable scientific centers such as the Human

Genetic Research Center Baqiyatallah Azam (AS).

Keywords: Ancient DNA, mtDNA, Genetic information, PCR


57

In silico study of the effect of two N-terminal

SNPs in E-cadherin protein on its structure,

function, and stability

Majid Tafrihi*

Department Molecular and Cell Biology, Faculty of Basic Sciences, University of Mazandaran, Iran


E-cadherin is a 120 kDa transmembrane protein that is

encoded by CDH1 gene and involved in cell-cell adherens

junctions. This protein is expressed in epithelial cells and

its downregulation is related to cancer cell invasion and

metastasis. There are many cancer-related polymorphisms

have been observed in this gene, that some of them affect

the protein structure and function. In this in silico study,

we examined the effect of rs878854691 (M1I) and

rs587780537 (G239R) single nucleotide polymorphisms

on the structure and function of E-cadherin protein. Our

analyses showed that rs878854691SNP is disease related.

The PhD-SNP server showed that this SNP is neutral.

Also, this substitution increased the absolute surface

accessibility and results in protein instability. Analyses

performed using Polyphen, PROVEAN and SNAP online

servers showed that this amino acid substitution has no

effect on the protein secondary structure.

Similar studies on the rs878854691 polymorphism showed

that this SNP is not disease-related and did not affect the

surface accessibility of this region. The rs878854691

polymorphism did not affect the protein function but the

MUpro showed the opposite results. On the other hand,

analyses performed using Port PARAM online tool

showed that this substitution results in significant decrease

in protein stability in vivo, but does not affect the protein

secondary structure.

Keywords: E-cadherin, Single nucleotide polymorphism,

Protein structure and function, Protein stability, In silico

Identification of genes involved in herbal

docetaxel-resistant prostate cancer

Alireza Tarinejad, Saba Sherkat Khabazi*

Department of Agricultural Biotechnology, College of Agriculture, Azarbaijan Shahid Madani University, Tabriz, Iran.


Docetaxel is an anti-cancer drug used to treat various

cancers, including breast, lung and prostate metastasis.

Docetaxel, by attaching to the microtubules, inhibits the

mitosis and thus leads to slow growth and release of cancer

cells. The purpose of this research is to identify the genes

involved in prostate cancer, to investigate biological

processes and to identify the genes involved in key

pathways. In this study, data on GSE47040 were analyzed

by Flex Array software and performed by T-test. The

analyzed data was filtered with Fold change (> 2, <-2).

Then, based on the abbreviation of the genes, biological

processes were involved and key pathways were identified.

Based on the analysis, 68 genes in treating with docetaxel

in the resistant cell line (TaxR) increased expression

versus the sensitive cell line (C4-2B) and 72 genes reduced

expression. By investigating the gene ontology of

identified genes, involved processes of biology included:

cellular process, response to stimuli, metabolic process,

immune process and biology (increased), and locative and

reproductive processes (reduced). Key genes identified in

different pathways included YWHAH, BCL3, CCL4L1,

and PRKCA.

Keywords: Gene ontology, Docetaxel, Metastases prostate

cancer, TaxR and C4-2B Cell lines

https://www.ncbi.nlm.nih.gov/projects/SNP/snp_ref.cgi?rs=587780537

58

Identification of genes involved in

enzalutamide-resistant prostate cancer

Alireza Tarinejad,Saba Sherkat Khabazi*

Department of Agricultural Biotechnology, College of Agriculture, Azarbaijan Shahid Madani University, Tabriz, Iran


Enzalutamide is the second generation of prostatic

hormonal medications and is used to treat docetaxel

resistance patients in advanced prostate cancer. This drug

reduces their growth inhibitory effect on testosterone (a

hormone required to grow cancer cells). The purpose of

this study was to identify the genes involved in prostate

cancer in treatment with enzalutamide and

dihydrotestosterone, to investigate their gene ontology and

identifying the genes involved in the key pathways of

cancer. In this study, data on enzalutamide and DHT were

extracted from NCBI GEO and analyzed using Flexarray

software, Expression Console, and altered genes were

detected by treatment with enzalutamide and DHT. Also,

by examining the gene ontology, the biological processes

involved were also identified. Based on the analysis, 29

genes were reduced by DHT treatment and decreased by

30 genes, which are involved in biological processes of

cellular expansion and cell differentiation, adjustment of

homeostasis, and endothelial cell migration regulation,

respectively. 22 genes were treated with

dihydrotestosterone and enzalutamide, increased

expression and 33 genes decreasing expression, which play

a role in biological processes for cell death and cell

migration, respectively. Information from biological

processes showed that dihydrotestosterone alone led to an

increase in cancer and, when treated with enzalutamide,

reduced cancer by activating the processes involved in

apoptosis.

Keywords: Enzalutamide, Dihydrotestosterone, Gene

ontology

Effect of silica-chitosan nanocomposite

encapsulated epigallocatechin gallate on

SKOV3

Leila Alizade1, Mohammad Rahmati Yamchi2*, Roya Salehi3, Elham

Ahmadi1 1 Department of Biotechnology, Faculty of Advanced Medical Science,

Tabriz University, Iran 2 Department of Clinical Biochemistry, Faculty of Medicine, Tabriz University, Iran 3 Department of Nanotechnology, Faculty of Advanced Medical Science,

Tabriz University, Iran * Corresponding author: [email protected]

Ovarian cancer is one of the most common gynecological

cancer among women. It has an inactive and asymptomatic

course which led to the lately diagnosis in the advanced

stages that cause cancer cells became resistant to the most

of the drugs. Epigallocatechin gallate is one of the

polyphenols which is derived from flavonol and have

antioxidative, antiproliferative and pro-apoptotic effect

against ovarian cancer cell lines. Chitosan is one of the

most attractive natural polysaccharides because of its

special characteristics such as biodistribution,

biodegradation, and low toxicity. In this study, Chitosan-

silica nanocomposites were synthesized through the sol-gel

method in the presence of APTES and glutaraldehyde as a

linker. Then, their absorbance and particle size were

characterized by Fourier transforms infrared (FTIR)

spectroscopy and TEM. Then, EGCG encapsulated in the

nanocomposite. SKOV3 cell lines were treated with free

and encapsulated EGCG. FTIR spectroscopy approved the

process of nanoparticle synthesis. Size of nanoparticles

was between 75-150nm. The survival rate of ovarian

cancer cell lines which treated with EGCG alone and

encapsulated EGCG was evaluated by MTT assay.

Encapsulated EGCG could decrease the survival rate in

SKOV3 cell line through a dose and time-dependent

manner and also it has less cell viability in contrast with

cells which treated with EGCG alone.

Keywords: Epigallocatechin gallate, Silica, Chitosan,

SKOV3


https://scholar.google.com/citations?view_op=view_org&hl=en&org=3726537592650218827

https://scholar.google.com/citations?view_op=view_org&hl=en&org=3726537592650218827

59

Study of the simultaneous coating of

electrospun nanofibers with bioactive

molecules for stem cell osteogenesis in vitro

Mehrdad Zahiri-Tous, Marzieh sadat Ahmadi*, Seyed Jalal Zargar, Ehsan

Seyedjafari Department of Cell & Molecular Biology, School of Biology, College of

Science, University of Tehran, Tehran, Iran


The loss of skeletal tissue that can accompany trauma,

injury, the disease can result in significant morbidity and

significant socio-economic cost and emphasize the need

for new, more reliable skeletal regeneration strategies. To

address the unmet need for bone augmentation, tissue

engineering that includes stem cell, scaffold, and growth

factor has come to the fore in recent years with new

approaches for de novo skeletal tissue formation. in order

to analyze the impact of bioactive factors onOsteogenic

differentiation and growth of Mesenchymal stem cells

three type of scaffolds were made including poly_L_

lactide acid scaffold coated with collagen, poly_L_ lactide

acid scaffold coated with particles of Hydroxyapatite and

poly_L_ lactide acid scaffold coated with both collagen

and nanoparticles of hydroxyapatite. After fabrication,

morphology and porosity of scaffolds checked using

scanning electron microscope (SEM). Then Mesenchymal

stem cells derived from adipose tissue were seeded on

scaffolds and the levels of attachment and growth

measured using MTT assay on days 3,5,7 after cell seeding

and last level of differentiation measured using osteogenic

indicators like alkalinephosphate enzyme activity (ALP),

amount of calcium sediments and expression of RUNX2

gene (RUNX2 protein acts as a "master switch," regulating

a number of other genes involved in the development of

cells that build bones). The overall results showed the

positive impact of electrospun poly_L_ lactide acid

scaffold that coated simultaneously with collagen and

hydroxyapatite on attachment, growth, anddifferentiation

of Mesenchymal stem cells toward osteogenic tissue

comparedto other groups.

Keywords: Osteogenic differentiation, Mesenchymal stem

cell, PLLA scaffold, RUNX2 gene

Clinicopathologic features in HER2-positive

breast cancer women in Kermanshah

Mehrdad Payandeh1, Reza Masoomi Jahandizi2*, Saba Yari3 1 Department of Hematology and Medical Oncology, Kermanshah University of Medical Sciences, Kermanshah, Iran.

2 Department of Cellular and Molecular Biology, Faculty of Science,

University of Maragheh, Maragheh, Iran.

3 MSc Graduated, Department of Cellular and Molecular Biology, Faculty

of Science, University of Maragheh, Maragheh, Iran


Breast cancer is the most frequent malignancy among

women with a peak in 40–50 years in Asia. Despite the

high frequency of breast cancer among Iranian women, the

epidemiological characteristics of breast cancer among

Iranian patients are yet unknown. The aim of this study

was to analyze clinicopathologic characteristics to identify

prognostic factors for women with HER2-positive breast

cancer in Kermanshah. Between 1393 and 1396, 70

patients with breast cancer, who were referred to

oncologist laboratories, Kermanshah, Iran were studied.

They were surveyed for age, size of the tumor, family

history of the disease, laterality, type of pathology, grade,

stage, tumor markers and metastasis. The mean age of

patients at diagnosis was 47.11±12.39 yearsold. Size of

tumor in 14 patients (20%) was 0.1-2 cm, 46 patients

(66.6%) between 2.1-5 cm and 9 patients (13.4%)>5 cm.

four patients (5.7%), 37 patients (52.9%) and 27 patients

(41.4%) had grade I, grade II and grade III, respectively.

30 patients (42.85%) had metastasis to other organs. 6

patients (8.57%) have a family history of any cancer. The

mean age at diagnosis of breast cancer in Kermanshah is

around 45 to 50 yearsold. Significant clinical features were

observed in the Kermanshah patients with HER2-positive

breast cancer. Furthermore, targeted anti-HER2 therapy

may improve the prognosis of these patients.

Keywords: Breast cancer, Epidemiology, Pathology,

HER-2 positive


60

The study of the effects of Cucurbitacin E

from Ecballium elaterium (L.) A. Rich on LC-

3 gene expression in human gastric cancer cell

line AGS

Naser Jafargholizadeh*, Seyed Jalal Zargar

Department of Cell & Molecular Biology, School of Biology, College of Science, University of Tehran, Tehran, Iran


Medicinal plants are both used as phytotherapy and to

isolate phytochemicals with interesting pharmacological

features. Ecballium elaterium is a plant indigenous to the

Mediterranean region that produces cucurbitacin

molecules. Cucurbitacins target several signaling pathways

and exhibit a range of anti-cancer functions. In this study,

we investigated the effects of cucurbitacin E purified from

E. elaterium fruits on LC-3 gene expression in AGS cell

line. Using quantitative reverse transcription polymerase

chain reaction (qRT-PCR), the expression of the LC-3

gene was quantified in AGS cells 24 hours after treatment

with cucurbitacin E. Purified cucurbitacin E upregulated

LC-3 in AGS cells (p-value <0.05). In conclusion,

cucurbitacin E purified from E. elaterium fruits

upregulates LC-3 gene in human gastric cancer cell line

AGS. The present study provides new insights into the

molecular mechanisms underlying cucurbitacin-mediated

cell death in gastric cancer.

Keywords: Cucurbitacin E, Cancer, LC-3 gene, E.

elaterium

Hepatocyte growth factor (HGF) serum

concentration and promoter polymorphism in

risk prediction of autism spectrum disorder

Masomeh Khalili1, Farhad Mashayekhi1, Elham Bidabadi2 1 Department of Biology, Faculty of Sciences, University of Guilan, Rasht, Iran 2 Faculty of Medicine, Guilan University of Medical Sciences, Rasht, Iran


Autism spectrum disorder (ASD) is a neurodevelopmental

disorder that characterized by impairments in social

communication and behavior. A number of environmental

and genetic factors are suggested to be involved in the

pathogenesis of ASD. Hepatocyte growth factor (HGF) is

suggested to be involved in the development of ASD. HGF

gene located on human chromosome 7 (7 q 21.11). HGF

and its receptor, c-MET, are expressed in the nervous

systems and regulate many aspects of neuronal physiology

including, plasticity, dendrite maturation, and patterned

connectivity. The study group consisted of 170 patients

with autism, (8 ± 3.8) and 120 healthy children (6.3 ± 3.7).

Genotypes and allele frequencies were determined by

polymerase chain reaction-restriction fragment length

polymorphism (PCR-RFLP). The concentration of HGF in

serum was measured by enzyme-linked immunosorbent

assay (ELISA). The genotype frequencies of CC, CT, and

TT in children with autism were 25.71%, 52.85%, and

22.42%, respectively, and in control group were 26.66%,

68.33%, and 5%, respectively (p<0.05). The allele

frequencies of C and T in children with autism were 0.52%

and 0.48%, and in the control group were 0.61 and 0.39%,

respectively (p<0.05). So the HGF TT genotype conferred

a 4.4-fold increased risk to ASD relative to the CC

genotype (OR=4.44, 95% CI=1.63 –2.41, P=0.003). HGF

T allele has also conferred increased risk to ASD relative

to the C allele (OR=1.42, 95%CI=01.00- 2.02, p=0.04). A

significant change in serum HGF concentration in ASD

patients was seen as compared to controls. It is suggested

that HGF serum concentration and its genetic variation are

involved in the pathophysiology of ASD.

Keywords: Polymorphism, Promoter, HGF, Serum

concentration, Autism


61

Study of MACC1 gene expression in blood

samples of patients with colorectal cancer in

west and northwest of Iran

Zohreh Taheri, Farokh Karimi*

Department of Genetics, Faculty of Basic Sciences, University of Maragheh, Iran


Colorectal Cancer (CRC) is the third most common cancer

in the world, with a high prevalence in women and men. In

the past three decades, its incidence has increased in Iran.

This cancer is usually seen in both hereditary and non-

hereditary forms. Today, different biomarkers have been

introduced for detection this cancer in early stages. The

purpose of this study was to evaluate the expression of

MACC1 gene as a metastasis metastasis biomarker in

people with colorectal cancer in west and northwestern

Iran. over expression of MACC1 by increasing the

expression of Met, β-catenin and its downstream genes,

including c-Myc, cyclin D1, MMP9, phos-GSK3β (Ser9),

MACC1, vimentin, and E-Cadherin HCT116 expression

suppression causes colorectal cancer. In this research,

blood samples were collected from patients with colorectal

cancer and healthy patients for control. After extracting

serum RNA from the MACC1 gene, its expression was

measured using RT-PCR. In the results, the increase in

MACC1 gene expression was observed in 67% of patients

with colorectal cancer, which indicates the important role

of MACC1 in carcinogenesis and CRC progression.

Similar to these results, have been reported in previous

studies. In conclusion, according to the results of this

research and previous studies, the MACC1 gene can be

considered as a biomarker for early detection of metastatic

colorectal cancer.

Keywords: Colorectal Cancer, MACC1, RT-PCR

Evaluation of APC gene mutation in ctDNA

of patient with colorectal cancer in northwest

of Iranwest and

Zohreh Taheri, Farokh Karimi*



Colorectal Cancer (CRC) is the third most common cancer

in the world. Over the past three decades, its incidence has

increased in Iran. This cancer is seen in both hereditary

and non-hereditary forms. Many researchers have

identified and investigated various biomarkers for

detection this cancer. One of the most important these

biomarkers are mutations in APC gene. It has been seen

that various types of mutations in this gene cause

colorectal cancer in 87% of cases. In this study, blood

samples were collected from patients with colorectal

cancer and healthy controls in the western and

northwestern region of Iran. After extraction of serum

ctDNA, mutations in the exon 18 of the APC gene were

evaluated using ARMS-PCR assay. The results showed

that mutations in this region of APC gene in males and

females with colorectal cancer were 19.2% and 20.1%.

Finally, the results of this study indicate that APC gene

can be used as a biomarker for CRC detection.

Keywords: Colorectal cancer, APC, ARMS-PCR, ctDNA



62

Y-chromosome identification in circulation

cell-free fetal DNA by PCR

Saeid Mohebbi1, Farrokh Karimi*



Prenatal diagnosis of fetal sex requires invasive methods

such as chorionic villus sampling (CVS) and

amniocentesis, which carry a risk of miscarriage of around

1% and can only be safely conducted after 11 weeks of

pregnancy. After the discovery of cell-free foetal

DNA/RNA (cffDNA/RNA) in maternal plasma in 1977,

the possibility to use this cffDNA/RNA for non-invasive

prenatal diagnosis (NIPT) has been investigated many

times. cffDNA has been found to be fragmented (smaller

than 200 bp) that in most cases originated from placental

trophoblast cells. cffDNA has been identified by a variety

of fetus specific markers, such as chromomosome Y-

specific sequences (SRY gene), epigenetic markers, and

SNPs. In this study, non-invasive determination of fetal

sex was performed by polymerase chain reaction (PCR),

and detection of Y-chromosome specific sequences (SRY

gene) in maternal plasma. Absence of Y-chromosome

sequences in maternal plasma implies that the fetus is

female. In this study, early determination of fetal gender

using cffDNA can be considered as a non-invasive pre-

test.

Keywords: NIPT, cffDNA, Sex determination, SRY gene,

PCR

In Silico studies of Congenital Adrenal

Hyperplasia (CAH), caused by CYP21A2 gene

mutation

Saeid Mohebbi1, Farrokh Karimi*



The Congenital Adrenal Hyperplasia (CAH), comprise a

family of autosomal recessive disorders that disrupt

adrenal steroidogenesis. The most common from is due to

21-hydroxylase deficiency associated with mutations in the

CYP21A2 gene. CAH is the most common cause of the

ambiguous genitalia in neonatal girls. In this study, the

effect of g.89C>T(P30L), g.655A/C>G(I2G) and

g.1683G>T(V281L) mutations in structure and function of

CYP21A2 expressed protein analyzed via an in silico

approach. NCBI, UniProt, and ExPASY databases were

used for access to the nucleotide and protein sequences.

Mapviewer tool showed that the CYP21A2 gene encoding

21-OH consist of ten exons which are located on the short

arm of chromosome 6 (6p21.3) in the class III region of

the major histocompatibility complex (MHC). CD search

and Motif scan program was used for detection of active

domain and the Cytochrome P450 domain was found.

Using the online servers of Psipred and RaptorX along

with Mega7 software, some analysis like prediction of

secondary and 3D protein structure and drawing of the

phylogenetics tree carried out. Phylogenetic tree was

designed to examine the evolutionary relationships of the

human CYP21A2 gene with other animal species. The

information in this study can be useful in developing a

method for diagnosis and control of the disease.

Keywords: CAH, Mutation, Bioinformatics

63

In silico modeling and characterization of L-

asparaginase from bacteria, plants and fungal

sources, using computational tools and online

servers

Ali Mohammadi1, Reza Mohammadzadeh1*, Mohammad

Mohammadzadeh2*, Mohsen Sagha 1 Department of Cell and Molecular Biology, Faculty of Science,

University of Maragheh, Iran 2 Research Laboratory for Embryology and Stem Cells, Department of Anatomical Sciences and Pathology, Faculty of Medicine, Ardabil

University of Medical Sciences, Ardabil, Iran


L-Asparaginase as a chemotherapeutic agent plays a very

important role in the treatment of patients with acute

lymphocytic leukemia )ALL), chronic myeloid leukemia

(CML) and Hodgkin's lymphoma. In this study, in order to

find resistant structures or less susceptibility to cysteine,

the first, second and third structure of the protein,

molecular weight and isoelectric point of the enzyme were

investigated in three sources of bacterial, herbal and

fungal . Asparaginase enzyme from the origin of

bacteria Escherichia coli and Campylobacter jejuni, an

herb of Withania somnifera and fungus, Fusarium

equiseti was studied. Amino acid sequences were extracted

from the NCBI site. The second structure was studied

using the software Psipred and the third structure through

SWISS-MODEL software. To evaluate the domain

function, PROSITE software was used. The molecular

weight, isoelectric point and the number and types of

amino acids of the asparaginase structure in the studied

origins were obtained from isoelectric.ovh.org site. The

results showed a significant difference in nucleotide,

amino acid, and the number of alpha and beta structures

and the third structures of protein in the studied sources.

But significantly, the asparaginase enzyme in the three

studied sources had functional indices such as the domain

performance, molecular weight, and the same isoelectric

domain, which could confirm the Codon usage

phenomenon. Regarding the sensitivity of this enzyme to

the cysteine-proteases, it is important to find the structures

of asparaginase without cysteine or low cysteine amino

acids in comparison with asparaginases with prokaryotic

and eukaryotic origin. Asparaginase enzyme in E.coli has

2 cysteines, C.jejuni without cysteine, 4 cysteines in

Withania somnifera and 6 cysteines in Fusarium equiseti.

In this study, by comparing the structures of asparaginase,

an appropriate structure for in vitro studies, such as

genome editing, has been attempted to produce a higher-

yielding drug. Investigating the structural and functional

characteristics of this enzyme can be useful in optimizing

the design of the drug and eliminating its side effects, in

addition to providing more information about functional

and structural characteristics.

Keywords: L-Asparaginase, Acute lymphoblastic

leukemia, In silico

Bioinformatics comparison ofSOX9, FOXP2,

DUF1220,APOC1,SIGLEC13,CLLU1, AQP7,

PDYN and HAR1genes in human and

chimpanzee

Reza Mohammadzadeh*, Zohreh Taheri



In the present era, with the development of advanced tools

such as molecular technique and bioinformatics software, a

new way has been developed in the study of the human

genome and other primates, as well as their genome

comparison, which can be the beginning of the golden era

for the molecular genetic study. Despite the fact that there

are more than 90% of the similarities between the human

genome and large monkeys, several millions of structural

and single nucleotide differences are also visible between

them. It seems that these differences are due to the

evolution of a new phenotype. In this study, using

bioinformatics data base and software, nine genes

including; SOX9, FOXP2, DUF1220, APOC1,

SIGLEC13, CLLU1, AQP7, PDYN and HAR1 in human

and chimpanzeecompared. The results show that these

genes in two species despite significant similarities, but

have seen nucleotide changes such as deletion, duplication,

increase in length, and changes in the number of copies of

the genes in them, which is likely to be in the direction

natural selection have been made for human reconciliation.

Keywords: Bioinformatics, Human, Chimpanzee, Natural

selection, Genetic

64

Contribution of the bHLH-transcription

factor gene family to male infertility: a

comprehensive gene prioritization analysis

Younes Aftabi1*, Abasalt Hosseinzadeh Colagar1, Faramarz Mehrnejad2 1 Department of Molecular and Cell Biology, Faculty of Basic Sciences, University of Mazandaran, Babolsar, Mazandaran, Iran 2 Department of Life Science Engineering, Faculty of New Sciences &

Technologies, University of Tehran, Tehran, Iran * Corresponding author: [email protected]

The bHLH transcription factors form a conserved gene

family in eukaryotes and play a crucial role in

differentiation, development, and signaling. Some

members of this family are highly expressed in the

mammalian reproductive system and control its molecular

events. Then, their malfunction could lead to inefficiency

of reproduction and infertility. However, exact knowledge

about the importance level of these genes in infertility

could be helpful for idea development and prevents

devoting sources to non-priority studies. For this purpose,

bioinformatics approaches provide beneficial

opportunities. Gene prioritization is an algorithm, which

works on the base of molecular data and identifies the

most promising associated genes with a biologic event or

disease among candidates. In the present research, 110

members of the human bHLH gene family (Candidates)

and four sets of male infertility associated genes (Training)

were deduced from the databases. Then, using gene

prioritization servers: ToppNet, ToppGene, pBRIT and

Endeavour, 20 sets of priority levels were produced.

Finally, ranking analysis revealed that NCOA1, NCOA3,

NCOA2, HIF1A, TCF3, MYC, AHR, and ARNT are the

most prioritized members of bHLH genes in association

with male infertility.

Keywords: Transcription factors, bHLH, Infertility, Gene

prioritization

In silico analysis of immune system

stimulation by asparaginase enzymes

produced by bacterial endophytes

Elaheh Zadeh Hosseingholi*, Neda Neghabi, Nader Chaparzadeh

Department of Biology, Basic Science Faculty, Azarbaijan Shahid Madani University, Iran


Unlike normal cells, cancer cells receive asparagine amino

acids from extracellular sources. In order to evacuate this

amino acid from the surrounding of cancerous cells, some

cancer patients receive the commercially available

asparaginase enzyme producing by Escherichia coli as a

medicine. Due to the immunogenicity of this enzyme,

allergic reactions and immune responses can be observed

in the patient's body. In recent years, regardless of

immunological characteristics, many studies have been

conducted on the production of this enzyme by endophyte

bacteria. In the present study, the possibility of production

of L-asparaginase with more appropriate immunological

parametersby this kind of bacteria was investigated

through bioinformatics methods. To investigate the

presence of this enzyme in endophytic bacteria BLAST-P

with E.coli asparaginase enzyme sequence against 275

endophytic bacteria were performed. The results with

identity more than 35%, coverage of more than 50%, and

E-value less than 4-10

were selected. To prediction of

allergenicity, antigenicity and the number B and T

lymphocytes epitopes, E.coli asparaginase enzyme

sequence and selected sequences were analyzed with

Algpred, Antigenic Peptide Prediction, VaxiJen, ABCpred,

ProPredI and ProPred software. According to the results, a

total of 9 sequences of known and hypothetical proteins

were identified in 6 bacteria. The comparison of

thementioned immunological characteristics of these

sequences showed that some of the asparaginase enzymes

produced by endophytic bacteria possess more suitable

immunological indices. Therefore, the therapeutic use of

these enzymes is possible.

Keywords: Asparaginase, Escherichia coli, Endophytic

bacteria, Bioinformatics software

65

In Silico design of multimeric antigen as a

highly immunogenic peptide vaccine against

Bordetella pertussis

Ebrahim Valipour1, Reza Valipour2 1 Department of Molecular Biology and Genetics, Faculty of Arts and Sciences, Bulent Ecevit University, Zonguldak, Turkey 2 Department of Biotechnology, Institute of Basic and Applied Sciences,

Cukuruva University, Adana, Turkey * Corresponding author: [email protected], [email protected]

Raising concern about adverse effects of whole-cell

vaccines against Bordetella pertussis has led to a reduction

in public vaccine acceptance and a consequent increase in

the incidence of the whooping cough disease. Thus, efforts

were made to develop acellular subunit vaccines based on

key virulence factors. One of the major advantages of

subunit vaccines is that they can be also used in older

children and adults. Adolescents and adults become

gradually susceptible, as demonstrated by the increased

incidence of atypical whooping cough cases in these age

groups. Therefore, in order to in-silico design of a good

recombinant chimeric vaccine against Bordetella pertussis,

the amino acid sequence of the pertussis toxoid and

pertactin were extracted from the NCBI and UniProtKB

databases. Then the high-level immunogenic and antigenic

regions of pertussis toxoid and pertactin were determined

using immunoinformatic programs. By assembling these

epitope-rich parts, a chimeric protein was designed that in

in-silico analysis, this protein exhibited a high ability to

induce the immune system against Bordetella pertussis.

Epitope predictions have shown that this hypothetical

structure can induce B and T cells and cause high immune

responses.

Keywords: Bordetella pertussis, Peptide vaccine,

Bioinformatics

Comparative evaluation of silibinin effect on

apoptosis in human breast cancer MCF-7 cell

line in vitro and in vivo

Zohreh Jahanafrooz, Ghazale Dini

Department of Biotechnology, Higher Education Institute of Rab-Rashid, Tabriz, Iran


Silibinin, a natural flavonoid from the seeds of milk thistle,

has been used for over 2000 years to treat a range of liver

disorders, because of its strong antioxidant effects. In

recent times it has been shown that silibinin has anti-

cancer activities, including growth inhibition, inhibition of

angiogenesis, cell cycle arrest, anti-proliferative effects,

apoptosis induction and inhibition of invasion and

metastasis. Due to its non-toxic character, silibinin is well

tolerated and largely free of any adverse effects. The aim

was to evaluate and compare the effect of silibinin on

apoptosis in human breast cancer cell line MCF-7 in vitro

and in vivo. For the first time, we evaluated the silibinin

apoptosis effect in MCF-7 cell line in vivo by CAM assay.

Cancer cells were grafted onto a chicken chorioallantoic

membrane (CAM) and xenografts were analyzed

immunohistochemically. The apoptosis was detected via

TUNEL assay.For comparison, we also performed 2D cell

culture apoptosis assay with Annexin/Pl with the same

concentration and time exposure. In 2D cell culture,

silibinin induced significant apoptosis cell death in MCF-7

cells. Flow cytometry experiments indicated 25.9 ± 1.8%,

p<0.05 apoptosis by both Annexin V+ and Annexin V+PI+

evaluations and 12±1.7 necrosis (only PI+) under 150 µM

silibinin supplementation at 48h. CAM assay results

confirmed apoptosis induction of silibinin in vivo. Our

study confirms the ability of silibinin to suppress breast

cancer progression through the induction of apoptosis.

Keywords: Silibinin, MCF-7, Apoptosis



66

Genetic evaluation of cold atmospheric

plasma, Jasmonic acid and Spermine

treatments on Catharanthus roseus (L.) seeds

by TRAP marker

Donya Fahmi1*, Zahra Noormohammadi1, Seyed Mohammad Atyabi2,

Farah Farahani3

1 Department of Biology, Science and Research Branch, Islamic Azad

University, Tehran, Iran 2 Department of Nanobiotechnology, Pasteur Institute, Tehran, Iran 3 Department of Microbiology, Qom Branch, Islamic Azad University,

Qom, Iran


Catharanthus roseus (L.) from the Apocynaceae family

produces more than 130 terpenoid indole alkaloids (TIAs),

including vinblastine and vincristine which are used as

anti-cancer drugs. These alkaloidal drugs are still in

clinical use and this plant is the only source of them. In the

present study, we used cold atmospheric plasma Jet to treat

C. roseus seeds and investigated the impact of Cold

Atmospheric Plasma (CAP) on genetic diversity using

Target Region Amplification Polymorphism (TRAP) as a

molecular marker. TRAP-PCR was performed by fixed

primer designed for TDC or STR genes which have a key

role on alkaloids biosynthesis pathway, and arbitrary

primers that target the ORF sequences. The C. roseus

seeds were divided into six groups: 1-untreated seeds as

controls, 2-treated group by cold plasma with 50 seconds

(the 50s), 3-treated seeds by Jasmonic acid, 4-treated seeds

by a combination of Jasmonic acid and cold plasma, 5-

treated seeds by spermine and 6-treated seeds by a

combination of spermine and cold plasma. Our results

showed genetic differentiation between 6 studied groups.

Among these groups, Cold plasma+ Jasmonic acid-treated

plants showed the highest genetic diversity values

including a number of effective alleles, Nei’s genetic

diversity, Shannon index and percentage of polymorphism

(Ne= 1.332, He= 0.249, I= 0.176 and P%= 39%). NJ

clustering showed three main groups. Each group

consisted of plants from different groups. Although TRAP

marker could show genetic variations, more molecular

markers are necessary to bring more genetic differentiation

among samples. However, hormone and cold atmospheric

plasma treatments revealed genetic changes on C. roseus

and may be potential sources for introducing genetic

variation in this medicinal plant.

Keywords: Catharanthus roseus, Genetic diversity, Cold

Atmospheric Plasma Jet, TRAP, Jasmonic acid, Spermine

A deep insight into the existed introns in the

18S rDNA gene of Dunaliella species

Azam Afaghi*

Department of Biology, University of Sofian, Iran * Corresponding author: [email protected]

The halotolerant green microalga Dunaliella by having a

high potential for the production of valuable

pharmaceutical compounds especially carotenoids as well

as the production of biofuels has been attracted the many

of researchers. Surprisingly, the study of 18S rDNA gene

in D. parva and D. salina showed that this gene

containsintron (s), belonging to group І of introns.

Accordingly, later studies revealed that the 18S rDNA

gene in D. tertiolecta is ~1770 and lacks intron, in D.

salina is ~2170 and has one intron after the first exon and

in D. parva and D. bardawilare ~2570 which associated

with two introns after the first and second exon

respectively (refer to intron I and II). However, the 18S

rDNA gene of D. viridis is ~ 2570 bp and has a larger

intron than the other Dunaliella strains between the first

and second exons. Despite the same size of 18S rDNA

gene, the capacity of β- carotene production is the only

character for separating D. parva, D. bardawil and D.

viridis, so that, these strains are hyper, low and none

producer of β- carotene respectively. Herein, we focus on

the introns and the insertion sites based on bioinformatics

approaches. The 18S rDNA sequences of Dunaliella

species and some members of Chlamydomonas (about 40

sequences) were obtained from the NCBI database.

Consequently, the data were studied by Bioedite and Mega

version 6 software.Analyses of different members of

Chlamydomonas order were showed the 18S rDNA gene

contains two exons with high conserved sequences. So

that, the difference between Dunaliella species and the

members of Chlamydomonas order were only 3 and 5

percent respectively. Alignment of the sequences was

showed the insertion site of the introns in the 18S rDNA

were highly conserved. Importantly, the aligning of the

sequences showed all of those begins with 5′ TTAAC and

terminate to AACGG 3′. These sequences exist in the

different genus with introns in their 18S rDNA gene.The

conservatory of the intron insertion sites in the 18S rDNA

of Chlamydomonas order is showed that we need to

explore the evolutional keys about this process. Moreover,

what is the main function of these introns? What is

happening that cause these introns, as the variable

elements, to be inheritable?

Keywords: Dunaliella, 18S rDNA gene, Intron,

Chlamydomonales



67

Presenting a novel method for classification of

Dunaliella species: a new approach, which

uses 18S ribosomal DNA, ITS and rbcL

molecular markers

Azam Afaghi*

Department of biology, University of Sofian, Iran


The genus Dunaliella (Dunaliellacae,

Chlamydomonadales) contains green bi-flagellate and cell

wall-less microalgae that exist in hypersaline environments

the members of this genus are known as photosynthetic

eukaryotes, which can grow in a wide range of salt

concentrations, varying from 0.05–5.0 M NaCl. Different

species of this genus possess unique features, biological

characteristics, and biotechnological potentials. Thus, it is

necessary to have a clear and reliable taxonomic method to

identify different species. Although several taxonomic

systems based on morphological, physiological and

molecular features exist for Dunaliella, none of these

methods is trustworthy enough and some controversies

exist between different strategies. In the current study,

molecular techniques and bioinformatics tools have been

used to re-evaluate the phylogenetic position of Dunaliella

species based on 18S ribosomal DNA (18S rDNA), ITS

and rbcL regions. The findings based on these markers

together provide a new and more reliable tool for the

phylogenetic analysis of Dunaliella species and strains.30

sequences of 18S rDNA, ITS (ITS 1 + ITS 2) and rbcL

markers about Dunaliella species that registered in NCBI

database were extracted for phylogenetic analyses by

MEGA 6 software. Consequently, Neighbour Joining (NJ)

and Minimum Evolution (ME) analyses were performed

by using the Maximum Composite Likelihood model with

1000 bootstrap replicates. Furthermore, the sequences of

18S rDNA, ITS 1, ITS 2 and rbcL of Chlamydomonas

reinhardtiiwas selected as an outgroup.Combined

phylogenetic analysis based on 18S rDNA, ITS and rbcL

were showed that D. lateralis strain Nepalhas high

divergenicitythan the other Dunaliella species. Moreover,

D. primolecta UTEX 1000 and D. bioculata UTEX 199

were clustered with D. tertiolecta strains. The other

ambiguous case is the phylogenetic position of D.

bardawil UTEX 2538. Based on morphological features of

D. bardawil UTEX 2538, it should be considered as D.

salina. However, our method strongly revealed that D.

bardawil UTEX 2538 was classified with D. bardawil

strain KMMCC 1346. All of the data were consistent with

the validated reports about Dunaliella

taxonomy.Conclusively, the present method offers more

validate to the system for accurate phylogenetic evaluation

of the Dunaliella genus. Altogether, additional

investigations are necessary to shed more light on

Dunaliella phylogeny and taxonomy.

Keywords: Dunaliella, 18S rDNA, ITS, rbcL

The effect of cold plasma, methyl jasmonate

and putrescine on genetic variation of

Catharanthus roseus (L.)

Mahnoush Mohammadzadeh Shahir1*, Zahra Noormohammadi1, Farah

Farahani2, Seyed Mohammad Atyabi3

1 Department of Biology, Science and Research Branch, Islamic Azad

University, Tehran, Iran 2 Department of Microbiology, Qom Branch, Islamic Azad University, Qom, Iran 3 Department of Nanobiotechnology, Pasteur Institute of Iran, Tehran


Catharanthus roseus is a medical plant belonging to the

family Apocynaceae. This plant plays a considerable role

in medicine for treatment of various diseases because of

production more than 130 terpenoid indole alkaloids.

Despite its importance, sources of the compounds are still

limited. Genetic changes would be a possible way to

increase the TIA productions. Sequence Related Amplified

Polymorphism (SRAP) is a novel molecular marker

system which is based on open reading frames (ORFs).

The purpose of this study was to evaluate the effect of cold

plasma jet and plant hormones on genetic variation. The

cold helium plasma jet operated at 13.5 KV and 50

seconds, for hormones treatment seeds were soaked in

methyl jasmonate (100 µM) and putrescine (100mg/L),

cold plasma + methyl jasmonate and plasma+ putrescine.

Genetic diversity was determined by using 10 primers of

(SRAP) marker. The results showed that the highest

genetic variation was for putrescine treated plants (Ne

=1.414, I = 0.299, He = 0.214 and P% = 44.44). Neighbor-

Joining and PCoA ordination based on SRAP data showed

the genetic distance between MJ treated plants and the rest

of the groups studied. Cold plasma treated plants spread in

four main NJ clusters. However, the SRAP markers

revealed low genetic variations because of its nature

(coding sequences). The further study is necessary

toevaluating the production of alkaloid components of

treated plants.

Keywords: C. roseus, SRAP, Putrescine, Methyl

jasmonate, Cold plasma


68

Investigation of repeatability of presence and

effect of the rs3918242 in patients with autism

spectrum disorder

Javid Rezaei1, Farhad Mashayekhi1, Elham Bidabadi2

1 Department of Biology, Faculty of Sciences, University of Guilan, Rasht, Iran 2 Faculty of Medicine, Guilan University of Medical Sciences, Rasht, Iran


Autism spectrum disorder (ASD) is a clinically

heterogeneous neurologic diseases collection characterized

by deficits in social and communication. There are some

essential genes including CNTNPA2 and MMP9. Matrix

Metalloproteinase-9 (MMP9) is expressed by astrocytes,

and microglia which play an important role in

neuroinflammation. In this project, we aimed to analyze

the association of MMP-9 (rs3918242) gene

polymorphism and its serum levels with ASD. 170 patients

with ASD and 125 controls subjects were enrolled in this

study. Genomic DNA was extracted from peripheral blood

samples using triton X100 solution kit and the MMP-9

nucleotide polymorphism were determined by polymerase

chain reaction restriction fragment length polymorphism

(PCR-RFLP). The serum level of MMP-9 was measured

by enzyme-linked immunosorbent assay (ELISA). The

results showed that the frequencies of CC, CT and TT

genotypes of MMP-9 were 67%, 31% and 2% in controls

and 31%, 57% and 12% in ASD, respectively (P=0.0001).

The frequencies of C and T allele in ASD patients were

59%, 41% and the controls was 83% and 17%,

respectively (P=0.0001). Significant association was

observed in genotypes and allele distributions of between

two groups (P<0.05). We have also showed that there is a

significant change in serum MMP-9 expression in ASD

patients as compared to controls. It is concluded that there

is a significant association between rs3918242 MMP9

gene polymorphism and serum levels of MMP-9 with

autism in the studied population. It is also suggested that

MMP-9 may play a role in pathophysiology of ASD.

Keywords: ASD, Polymorphism, MMP9, rs3918242

The study of resistin gene expression changes

in adipose and stomach tissues of male rats

subjected to chronic immobilization stress

Shadi Babaei1, Masoumeh Asl Rousta2*, Sanaz Mahmazi1 1 Department of Genetic, Zanjan Branch, Islamic Azad University, Zanjan, Iran 2 Department of Physiology, Zanjan Branch, Islamic Azad University,

Zanjan, Iran * Corresponding author: [email protected]

Chronic immobilization stress disrupts the function and

physiology of the body. Resistin is a hormone that

produced from adipose tissue. Increasing the expression of

resistin gene is associated with metabolic syndrome. The

aim of this study was to determine the changes in the

expression of resistin gene in the adipose and stomach of

tissue rats exposed to chronic immobilization stress. 10

male Wistar rats were divided into groups of Control and

chronic immobilization stress. Rats group of chronic

immobilization stress were exposed to 6 hours for 21

consecutive days. At the end of this period, animal adipose

and stomach tissues were removed. Further, RNA

extraction and cDNA synthesis were performed and

changes in the expression level of resistin gene in these

tissues were evaluated quantitatively by Real-Time

PCR.The results of the data analysis show that chronic

immobilization stress increased the expression of resistin

gene in adipose tissue and decreased its expression in the

stomach tissue compared with the control group. Chronic

immobility stress causes a metabolic syndrome with

resistin gene disruption. Chronic immobility stress has

increased resistin in adipose tissue. Increasing resistin

causes changes in glucose levels and increased insulin

resistance. The most important effects of resistin increase

are inflammation. Chronic immobilization increases the

inflammation of the rats by increasing the expression of

resistin.

Keywords: Resistin, Chronic immobilization stress, Rat

69

The Effect of hydroalcoholic extract of

spinach on leptin gene expression changes in

adipose and muscle tissues of male rats

subjected to chronic immobilization stress

Ghazaleh Farhadi1, Sanaz Mahmazi1*, Mahdi Rahnama2 1 Department of Genetic, Zanjan Branch, Islamic Azad University, Zanjan, Iran. 2 Department of Physiology, Zanjan Branch, Islamic Azad University,

Zanjan, Iran. * Corresponding author: [email protected]

Leptin gene regulates stability, energy, and metabolism,

and it is most important function is to reduce body weight

by reducing appetite and reducing feed intake and

increasing energy production from body fat stores. Stress

disrupts the function and physiology of the body and

changes the secretion of related hormones in different

tissues. Spinach has beneficial properties, including

cholesterol reduction and anti-anxiety, and is used to treat

many diseases. The present study examined the effect of

spinach extract on leptin gene expression changes in

Adiposeand Muscle of rats under chronic immobilization

stress. 30 male Wistar rats were divided into 6 groups: 1)

Control group. 2 and 3) Spinach extract treatment group

with 200 mg/kg and 400 mg/kg. 4) chronic immobilization

stress group. 5 and 6) chronic immobilization stress group

with spinach extract at 200 mg/kg and 400 mg/kg. Group

2, 3, 5 and 6 mice received hydroalcoholic extract of

spinach for 21 consecutive days. Mice Group 4, 5 and 6 of

6 hours for 21 consecutive days were bound. After the

Adipose and Muscle tissue separation, RNA extraction and

cDNA synthesis were performed and the changes in leptin

gene expression in these tissues were quantitated using

Real-Time PCR. Data analysis showed that the level of

leptin gene expression in adipose tissue was significantly

increased by chronic immobilization stress. But the

spinach extract reduces this change of expression. In

muscle tissue, leptin expression is reduced by immobility

stress and spinach extract increases its rate. Leptin, which

secretes from the adipose tissue, is a controlling factor in

metabolism. The male spinach extract has moderated its

expression level. Therefore, it may be possible to spinach

as a substance to prevent the effects of stress.

Keywords: Chronic immobile stress, Spinach extract,

Leptin gene

CXCL8 (IL-8) genetic variation (rs4073) in

the patients with ASD in Guilan population

Reza Javan1, Farhad Mashayekhi1*, Elham Bidabadi2 1 Department of Biology, Faculty of Sciences, University of Guilan, Rasht, Iran 2 Faculty of Medicine, Guilan University of Medical Sciences, Rasht, Iran


Autism spectrum disorders (ASD) alludes to a deep-rooted

condition that typically shows up in early youth, and is

described by a gathering of neurodevelopmental conditions

portrayed levels of incapacity. Many genes including

Neuropilin-2, methionine synthase, SHANK3 and CXCL8

(IL-8) were shown to play a key role in the development of

ASD. The location of the IL-8 gene in humans is on

chromosome 4. Changes in the serum IL-8 concentration

and expression were shown in ASD. The aim of this study

was to investigate the relationship of IL-8 gene variation

with ASD. A total of 100 autistic children and 120

nonautistic children were included in this study. DNA was

extracted from peripheral blood (leukocytes) using the

Triton X 100 extraction method. Extracted DNA was

confirmed by electrophoresis on 1% agarose gel

containing safe stain. For genotyping of the CXCL8

polymorphism (rs4073), polymerase chain reaction-

restriction fragment length polymorphism (PCR-RFLP)

method was performed using MfeI. The frequencies of AA,

AT and TT in autistic children were 27%, 44%, and 29%,

respectively, while in controls were 10%, 44/16% and

45/83, respectively. The allele frequencies of A and T in

autistic children were 49% and 51%, and in controls were

32% and 68%, respectively. Statistical analysis showed

that there is significant association in CXCL8 (rs4073)

gene polymorphism between patients and control groups.

AT genotype seems to be the protective factor in our

population (P=0.013, OR 0.36, 95% CI (0.167-0.812). The

results showed that there is a significant association

between CXCL8 (IL-8) genetic polymorphism and autism

in our population.

Keywords: CXCL8, rs4073, Genetic variation, Autism

spectrum disorders


70

Insulin-like growth factor-1 circulating

concentrations and Promoter Polymorphism

in Risk Prediction of children with autism

spectrum disorders

Mahsa Abedini1, Farhad Mashayekhi1*, Elham Bidabadi2 1 Department of Biology, Faculty of Sciences, University of Guilan, Rasht, Iran 2 Faculty of Medicine, Guilan University of Medical Sciences, Rasht, Iran


Autism spectrum disorder (ASD) is a set of

neurodevelopmental disorders characterized by a deficit in

social behaviors and nonverbal interactions such as

reduced eye contact, facial expression. It is not a single

disorder, and it is broadly considered to be a multi-

factorial disorder resulting from genetic and non-genetic

risk factors and their interaction. One of the genes is likely

to be the insulin-like growth factor-1 (IGF-1) that is

located on the long arm of chromosome 12q23–23 which

is known to have diverse effects on brain structure and

function. The aim of this project was to study the

association of IGF-1gene (rs 12579108) polymorphism

and its serum levels with autism patients. DNA was

extracted from blood samples of 51 patients (28 boys and

23 girls) and 50 people as a control group (27 boys and 23

girls) and genotyped by polymerase chain reaction-

restriction fragment length polymorphism (PCR-RFLP).

IGF-1 serum concentration was studied by enzyme-linked

immunosorbent assay (ELISA).The results showed that the

incidences of AA, AC and CC genotypes of were 0%, 88%

and 12% in controls and 2%, 22% and 76% in ASD

patients, respectively (P=0.0001). The allele frequencies

of A and C in the control group were 40% and 60% and in

ASD patients were 12% and 88 %, respectively P=0.0001).

A significant change in serum levels of IGF-1 was also

found in ASD patients as compared to normal controls.

Our results suggest IGF-1 serum levels and polymorphism

as potential independent prognostic markers for

susceptibility to ASD.

Keywords: Autism, IGF-1, Polymorphism

Bioinformaticsanalysisoflong- non-coding

RNA (LncRNA) in azoospermia

Fatemeh Rajabi Dehnavi*, Majid Motovali-Bashi, Seyed-Morteza

Javadirad Genetic Division, Department of Biology, Faculty of Sciences, University

of Isfahan, Iran


Azoospermia is the cause of about 20% of infertility in

men and disorder in regulating the expression of genes

involved in fertility can be effective in the creation of this

problem. A high percentage of transcripts within a

eukaryotic cell are non-coding and regulatory, and these

regulatory molecules include miRNAs and LncRNAs. One

of the tasks of LncRNAs is the regulation of miRNAs

balance, and by removing LncRNAs, the effect of

miRNAs on target mRNAs increases. In this study,

functional miRNA (hsa-miR-7-1-3p) in testicular tissue of

azoospermic individuals were identified by using the HMDD

database. It should be noted that the target genes of this

miRNA were previously predicted via TargetScan and

DAVID databases. Then using the NONCODE and

DIANA databases and with the help of LncBase v.2, the

hsa-miR-7-1-3p interaction with probable LncRNA was

investigated. The results from the databases showed that

IPW as an LncRNA is very likely to have negative

regulatory effects on hsa-miR-7-1-3p.It can be predicted

that IPW has been reduced in azoospermia and as a result,

the expression of miRNAs affected by it increases. Finally,

reduced expression occurs in Rb1 and Pik3r3 as target

genes of has-miR-7-1-3p. These target genes are expressed

in testicular tissue and play a crucial role in apoptosis of

the germ cells. By decreasing the expression of these

genes, the number of non-differentiated sperm increased

and as a result azoospermia occurs. Therefore, it may be

possible to use these regulatory molecules as new

biomarkers to diagnose azoospermia.

Keywords: Azoospermia, LncRNA, miRNA


71

Prediction of the effect of hsa-miR-3680-3p

and hsa-miR-671-5p on azoospermia

Fatemeh Rajabi Dehnavi*, Majid Motovali-Bashi, Seyed-Morteza Javadirad

Genetic Division, Department of Biology, Faculty of Sciences, University

of Isfahan, Iran * Corresponding author: [email protected]

Spermatogenesis is a significant process involving several

stages of proliferation, differentiation and cell death. Any

factor that disrupts each of these steps can lead to

disruption of sperm production and cause problems like

azoospermia. Azoospermia or lack of sperm in semen is

one of the main causes of male infertility, which can be

due to obstructive of the sperm duct or a genetic defect in

the production of sperm. One of the disrupters of

spermatogenesis is an aberrant expression of miRNAs.

miRNAs are a class of regulatory non-coding RNAs that

have about 21-25 nt. These RNAs control gene expression

by targeting mRNA or repression of translation. In this

bioinformatics study, two key genes in spermatogenesis

including crem and Bmp8b were selected by using KEGG

software and corresponding literature mining. We

predicted possible interactions between these genes and

miRNAs (expression of these miRNAs in testicular tissue

confirmed by TissueAtlas database), by using

computational tools such as miRwalk2.0, TargetScan,

picTar, andmiRanda. Based on studies, increase in

expression level of hsa-miR-3680-3p and hsa-miR-671-5p

in testis, these miRNAs may interact with transcripts of

crem gene and Bmp8b gene, respectively and by inhibiting

these two genes, the spermatogenesis is inhibited and

probably causing disorders including azoospermia.

Therefore, considering the role of these miRNAs in

inhibiting the crem and Bmp8b genes (which play an

important role in spermatogenesis), these miRNAs may be

used as pharmaceuticaland therapeutic potentials and

azoospermic biomarkers.

Keywords: Azoospermia, miRNA, Biomarker

Structure and distribution of WD40 genes in

sunflower (Helianthus annuus L.)

chromosomes

Masomeh Tartifi1*, Karim Sorkheh1, Khosro Mehdi-Khanlo1, Ilker

Buyuk2

1 Department of Agronomy and Plant Breeding, Faculty of Agriculture,

Shahid Chamran University

2 Department of Biology, Faculty of Science, Ankara University, Ankara, Turkey


The WD40 protein family plays an important role in

response to abiotic stresses. This research led to the

identification of 266 WD40 genes in sunflower. CDS

sequences and genomic sequences were extracted from the

helia gene database. The position of the intron and exons

each of WD40 genes was then determined using the gsds

database. In additions, the physical location of genes,

including the length of the gene, the starting point, and the

end of the point was determined and so mapped on 17

sunflower chromosome using Mapchart software. The

results of the analysis showed that chromosome 4 had the

highest number of WD40 genes and chromosome 7 had

the lowest number of WD40 genes.

Keywords: Transcription factor, WD40, Sunflower,

Chromosome, Gene structure



72

Assessment of relationships of phylogenetic

WD40 protein in sunflower (Helianthus

annuus L.) by a bioinformatics approach

Masomeh Tartifi1*, Karim Sorkheh1, Khosro Mehdi-Khanlo1, Ilker

Buyuk2

1 Department of Agronomy and Plant Breeding, Faculty of Agriculture,

Shahid Chamran University, Iran 2 Department of Biology, Faculty of Science, Ankara University, Ankara, Turkey


Transfusion factors play an important role in many

biological processes such as developmental, physiological

and chemical changes. Also, WD40 is considered to be

very important in tolerance to abiotic stresses (drought,

salinity, cold) and important cellular pathways, such as the

transmission of stress signals, RNA processing, cell

division regulation, especially transcriptional regulation in

chromatin. The aim of this study was to investigate the

phylogenetic relationships of WD40 protein in sunflower

plant. The phylogenetic tree of WD40 family genes was

divided into five groups. In addition, 266 WD40 genes

identified in sunflower are classified into 12 sub-families

according to their composition, which plays an important

role in responding to stress and stress tolerance in

sunflower.

Keywords: Transcription factor, WD40, Sunflower

Bioinformatics investigation of the structure

and function of mnemiopsin2 following

proline 181 substitutions using a site-directed

mutagenesis

Maryam Hosseinnia*, Vahab Jafarian

Department of biology, Faculty of science, University of Zanjan, Iran * Corresponding author: [email protected]

Mnemiopsin2 belongs to the family of calcium-regulated

photoproteins, which like other photoproteins, begins to

emit light in the presence of substrates such as

coelenterazine, molecular oxygen, and calcium initiator

ion. Proline 181 in mnemiopsin is the eleventh residue of

the EF-hand IV loop. Since EF-hand IV is one of the

active loops in binding to calcium, we replaced the proline

181 with residues of alanine (neutral, in order to reduce the

space interference of this position), lysine (a positive

charge residue, in order to strengthen the dipolar moment

at C terminus), and aspartic acid (a negative charge residue

in order to follow the first structure of aequorin

photoprotein in the same position). Thereafter, the effect of

these changes was evaluated on mnemiopsin2 function.

For this, the mutation models were designed using the

Moderller9v19 software. Then, the best wild and mutated

models were selected and evaluated using ModEval,

SAVES, SPdbViewer software and PIC Server. In the

following, the status of interactions and biochemical-

physical properties of wild and mutated proteins were

investigated. The 3D structure was also designed by

Chimera software. The results of the interactions analysis

by the PIC server have been shown to that the hydrophobic

interactions between the main chain and the mutated

P181D have decreased, and in two other mutations, the

increase in the wild type. The study of ionic interactions

has shown that the mutation in P181A decreased and the

two mutants showed an increase. Also, the index of

Instability Index derived from the Prot Param server has

shown that to enhance the structural stability all of the

three has been mutations.

Keywords: Photoprotein; Mnemiopsin2; Site-directed

mutagenesis; Proline 181 residue; Stability


73

Effect of Valine 172 residue alteration using a

site-directed mutagenesis on the structure and

function of mnemiopsin2: a bioinformatics

study

Ashraf Asadi*, Vahab Jafarian, Khosrow Khalifeh, Maryam Hosseinnia

Department of biology, Faculty of science, University of Zanjan, Iran * Corresponding author: [email protected]

Calcium-regulated photoproteins have been used as

appropriate tools for intracellular and extracellular studies

due to their unique properties, such as high sensitivity,

low-level background signal, etc. Mnemiopsin2 is a

member of calcium-regulated photoproteins, which like

other photoproteins, emits light in the presence of

substrates such as coelenterazine, molecular oxygen, and

calcium initiator ions. The residue of valine 172 is the

second residue in the EF-hand IV loop of mnemiopsin2.

Since the EF-hand IV loop is one of three active loops for

calcium binding, valine 172 was replaced with alanine

residue (as a neutral residue, in order to reduce the spatial

interference of this position), isoleucine and leucine (as the

residues similar to valine in terms of the side chain amino

acid), and the effect of these changes was evaluated on

mnemiopsin2 performance. For this, the mutation models

were designed using the Modeller 9v19 software, and the

best model was selected using ModEval, SAVES,

SPdbViewer software and PIC Server. After that, the

three-dimensional structure of the mutants was designed

using Chimera software. The investigation of the

interactions via the PIC server has been shown to increase

only in the mutation of the V172A hydrophobic

interactions. In all three mutations, the main chain

reactions, ionic and aromatic decreased, as well as the

main chain-side chain and cationic interactions in all three

mutated increase. The results of the Instability Index index

obtained from the Prot Param server indicate a decrease in

the stability of the mutations, which seems to be due to the

replacement of the side chain of the residues.

Keywords: Mnemiopsin2, Site-directed mutagenesis,

Structural stability, Valine 172

Comparison of TCEB3 gene expression

between breast cancer tissues and the

adjacent non-tumor tissues

Mina Zafarpiran, Mohammad Khalaj-kondori*

Department of Genetics, Animal Biology Group, Faculty of Natural Science, University of Tabriz, Tabriz, Iran


As the most common cancer among women in the world,

breast cancer needs more to determine biomarkers for

diagnosis and prognosis of it. Elongin A (ELOA or

TCEB3) is one of the subunits of RNA polymerase II that

potently activate the rate of it s transcription elongation.

Elongin is a multimeric elongation factor comprising three

subunits, Elongins A, B, and C. Here we aimed at

evaluation of elongin A or TCEB3 expression in breast

cancer. So, total RNA was extracted from twenty-five

pairs of breast tumor tissues and their marginal normal

tissues using RNX-plus reagent and also their cDNA was

synthesized by PrimeScriptTM

RT reagent kit(Takara)

according to the manufacture's instructions. Expression of

this target gene was evaluated by quantitative RT-PCR. In

this study, GAPDH was used as an nternal control gene.

Results showed significantly overexpression in tumoral

tissues compared to the paired non-tumoral samples.

Moreover, our findings showed that there was sa tatistical

significant correlation between clinical-pathological

features of tumors and ethe xpression level of TCEB3.

TCEB3 expression was upregulated in ER+, PR

+ and her2

+

breast cancer subtypes.

Keywords: Breast cancer, TCEB3, q-RT-PCR,

Overexpression


74

Microarray s gene expression analysis in

breast cancer using system biology

approaches

Mina Zafarpiran, Mohammad Khalaj-kondori* Department of Genetics, Animal Biology Group, Faculty of Natural

Science, University of Tabriz, Tabriz, Iran.


In recent years, cancer research has benefited from a large

number of available high throughputs gene expression

datasets. The results of such studies suggest new candidate

biomarkers for cancers. The NCBI gene expression

omnibus (GEO) datasets are being used to specify the list

of differentially expressed genes (DEGs) between cancer

tissues and the adjacent non-tumor tissues. Breast cancer

as the most common cancers among women is the main

target of this study. GSE103512 (ductal breast cancer, 49

samples) was selected from GEO datasets and the

preprocessing and normalization steps were performed on

the downloaded CEL files (raw data) using an AffylmGUI

package in R software version x64 3.2.2. After

normalization, the probe IDs were mapped to gene

symbols according to AffyMetrix annotation files provided

for each platform. The Limma package in AffylmGUI was

applied to identify the set of DEGs in our target cancer.

DEGs with adjusted p-values ≤ 0.05 were considered as

significant. Subsequently, DEGs with logFC (fold change)

values ≥1 or 1≤ that respectively correspond to a two-fold

increase and decrease in the expression levels were

selected. Functional enrichment analysis was carried out

for the identified DEGs using the Database for Annotation,

Visualization and Integrated Discovery (DAVID) tool. In

total, 2524 DEGs with 395 down genes and 105 up genes

were detected in breast cancer. Also, differential co-

expression analysis was performed using the ARACNE

algorithm. The results of the differential expression study

introduced new genes in breast cancer and provided better

insights into the molecular characteristics of this

malignancy.

Keywords: GEO, GSE103512, AffylmGUL, Limma,

LogFC, DAVID

Expression of miR-7, miR-409 and miR-93 in

patients with colorectal cancer who referred

to Tehran hospitals by Real Time PCR

Robabe Narimani, Hossein Soltanzadeh*

Bonab Islamic Azad University, Iran * Corresponding author: [email protected]

Neoplasm including colorectal cancer is often diagnosed in

the late stages and is dumped with weak prognosis.

Although tumor markers greatly improve diagnosis, but

there is numerous problem yet because of the nature of

invasive, inconvenient and troublesome of the current

methods. Hence, there is an urgent need to identify non-

invasive biomarkers for early detection of cancers. Also,

recently the changes in miRNA expression in various

cancers have been reported. Present study was designed to

investigate the relationship between the expression of miR

and colorectal cancer. The expression level of miR-7, miR-

409 and miR-93 in patients with colorectal cancer and

control groups was assessed by Real Time PCR.The study

was included 30 patients with colorectal cancer and 30

healthy individuals with no history of colorectal disease.

The age and conditions of the patient group was

conducted. In order to examine the expression level of

miR-7, miR-409 and miR-93 in patients with colorectal

cancer and healthy people qRT-PCR techniques were used.

First, RNA was extracted from plasma samples and finally,

using the qRT-PCR, expression levels of miRs were

measured and compared with the control group. By doing

statistical analysis (t-Student Test) changes of miRs

expression in patients and controls group was

analyzed.After statistical analysis, results showed that the

expression levels of miR-7 and miR-409 in the plasma of

patients with colorectal cancer was significantly higher

than the controls group, 4.3 and 4.2-fold respectively. Also

did not observed significant change between patients and

controls group in expression levels of miR-93.The results

showed that over-expression of miR-7 and miR-409 in

plasma may be associated with colorectal cancer. These

miRs probably have had the potential to use as the

noninvasive diagnostic markers to detect colorectal cancer.

Keywords: Non-Invasive Biomarker, Rapid Diagnostic

Test, miRNA


75

Association between SGSM3 gene (rs

17001868) polymorphism and breast cancer in

East Azarbaijan population

Elham Behruz1, Mohammad Reza Alivand2*, Hossein Soltanzadeh1 1 Bonab Islamic Azad University, Iran 2 Tabriz University of Medical Sciences, Iran


Breast cancer is one of the most common types of cancer

that causes many deaths among women and men every

year. Despite the many advances that have been made in

early diagnosis and proper treatment of this disease, the

most common causes of death are breast cancer among

women. According to Iran's statistics, in our country every

10 to 15 women have a chance of having breast cancer.The

aim of this study was to investigate the association of

SGSM3L-dependent rs17001868 polymorphism with

breast cancer in the East Azarbaijan population. In this

study, 100 blood samples of patients with breast cancer

and 100 blood samples from healthy persons as control

group were selected. Then DNA was extracted from all

samples using kit of DNA extraction according to the

protocol. Then, in order to ensure the quality of the DNA

extracted, electrophoresis was performed and quantitated

with spectrophotometer. In the next step, the specimens

were amplified by PCR and electrophoresis was performed

again. Ultimately, PCR products were treated with the

restriction enzyme Ssp 1 (Sphaerotilus species) and

electrophoresed on the agarose gel and the polymorphism

was observed as bands. Data were analyzed by SPSS

software Version 21 and evaluated by descriptive and chi-

square test. The level of significance was less than 0.05.

The results of this study indicate that the percentage of

allele A is higher in healthy people, but the difference

between healthy and patient is not significant. There is not

probably a correlation between the increase in the A allele

and the incidence of breast cancer. Therefore, the

relationship between rs17001868 polymorphism and breast

cancer can be revealed through more detailed studies.

Given that current treatments for treating various types of

cancers have serious complications, the discovery of new

methods for early detection of the disease by identifying

the specific biomarkers is essential and could open new

therapeutic approaches.

Keywords: Breast Cancer, Polymorphism, rs17001868,

SGSM3

The study of IDOL gene expression changes

in adipose tissue of male rats subjected to

chronic immobilization stress

Raana Hasanlo1, Sanaz Mahmazi1*, Mahdi Rahnama2 1 Department of Genetic, Zanjan Branch, Islamic Azad University, Zanjan, Iran. 2 Department of Physiology, Zanjan Branch, Islamic Azad University,

Zanjan, Iran. * Corresponding author: [email protected]

Immobilization stress is effective on physiological

systems. So that it increases, the risk of cardiovascular

disorders and disturbs cholesterol and increases the level

of LDL in the blood. IDOL is a protein that regulates

cholesterol and LDL levels by decomposing LDL

receptors. IDOL reduces the absorption of LDL from the

blood and increases its serum levels. The aim of this study

is to determine the changes in IDOL gene expression in

adipose tissue of rats exposed to chronic immobilization

stress. 10 male Wistar rats were divided into groups of

Control and chronic immobilization stress. Rats group of

chronic immobilization stress were exposed to 6 hours for

21 consecutive days. At the end of this period, animal

adipose tissue was removed. Further, RNA extraction and

cDNA synthesis were performed and changes in the

expression level of IDOL gene in this tissue were

evaluated quantitatively by Real-Time PCR. Based on the

results, immobility stress reduced the expression of IDOL

gene in adipose tissue of rats. Because IDOL gene

expression has been reduced due to immobility stress,

cholesterol and LDL cholesterol levels are likely to be

lower in this group. The results show that the effects of

immobility stress are not similar to low-mobility

complications.

Keywords: Idol gene, Chronic immobilization stress,

Adipose tissue

http://www.bonabiau.ac.ir/en/default.aspx

https://portal-en.tbzmed.ac.ir/

76

The effect of simulated microgravity on RKIP

tumor suppressor gene expression in MCF-7

breast cancer cell line

Maryam Salavatifar*

Space biology and Environment Center, Aerospace Research Institute, Ministry of Science, Research and Technology, Tehran, Iran


The living organisms on the surface of the earth are

affected by the natural gravity force (1g) and if this

gravitational force undergoes changes, it will surely be

under the influence of a unique shock and make changes to

accommodate it. Weightlessness exerts exhibitiveeffects

on cell functions by participation with biochemical

pathways and gene expressions and study of these

alterations would be beneficial to aid astronauts and

improving the quality of human life. It has been shown

that in simulated microgravity, the expression of some

genes and protein levels produced in cultured cells or

laboratory animals have been altered. However, very little

information is available on the effects of simulated

microgravity on the gene expression. Raf kinase inhibitory

protein (RKIP) is a regulator of kinase activity and a cell

balancing agent that also acts as a metastatic inhibitor in a

variety of solid tumors, including breast cancer, and has

diverse physiological functions. Overall, RKIP expression

in progressive tumors is reduced and its increase decreases

the invasive potency of cancer cells without affecting

primary tumor growth. In this study, we investigated the

changes of RKIP gene expression in human MCF-7 breast

cancer cells after 24 and 72 hours exposure to microgravity

conditions. Our consequences show that microgravity has

altered the expression levels of RKIP gene. In summary,

microgravity is a valuable instrument for prospecting new

aims in cancer therapy and can be simulated in some

aspects in ground-based conditions.

Keywords: Weightlessness, Microgravity, RKIP gene,

MCF-7 cells

Evaluation of nerve growth factor (NGF)

methylation status in patients with

schizophrenia

Amir Charkaneh*, Zivar Salehi, Robabeh Soleimani, Farzam Ajamian


Corresponding author E-mail: [email protected]

Nerve growth factor (NGF) is the first and best-

characterized member of the neurotrophin family. NGF

abnormality may be potentially involved in cognitive

deficits, such as those observed in schizophrenia. This

hypothesis is supported by the finding of decreased plasma

levels of NGF in schizophrenia patients compared to

healthy controls. Although genetic factors are risk factors

for schizophrenia, some environmental factors are thought

to be required for the manifestation of the disease.

Epigenetic mechanisms regulate gene functions without

causing a change in the nucleotide sequence of DNA. It is

established that methylation status of the NGF gene is

associated with fear learning, memory, and stressful social

interactions. This study included 30 patients (20 male and

10 female) with schizophrenia and 40 unrelated healthy

controls (20 male and 20 female). Determination of

methylation pattern of CpG islands was based on the

principle that bisulfite treatment of DNA results in the

conversion of unmethylated cytosine residues into uracil,

whereas methylated cytosine residues remain unmodified.

Methylation-specific PCR was performed with primers

specific for either methylated or unmethylated DNA.

Statistical analysis was performed using the chi-square

test. No substantial difference between patient and control

groups was found (p>0.05). In conclusion, our finding in

this research suggests that the hypermethylation of NGF

promoter isn't related to schizophrenia formation.

However, longer studies with more patient and controls are

needed to confirm this result.

Keywords: Schizophrenia, Methylation, Promoter, CpG

islands, NGF


77

Determination of 3D structure and properties

of cytochrome P450 enzymes in

entomopathogenic fungus Beauveria bassiana

Maryam Rashki1*, Mojtaba Mortezavi2 1 Department of Biodiversity, Institute of Science and High Technology and Environmental Sciences, Graduate University of Advanced

Technology, Kerman, Iran 2 Department of Biotechnology, Institute of Science and High Technology and Environmental Sciences, Graduate University of

Advanced Technology, Kerman, Iran


To view the motifs in the sequence of the cytochrome

P450 amino acids in the pathogenic fungus, Beauveria

bassiana, the MOTIF Search site was used and then the

motifs were further examined with the Weblogo.v.2.8.2

program. In all sequences, a motif has identified including

about 200 amino acids that are related to the P450. This

motif was in the sequence 45-96 to about 477-520. The

EXXRin helix K and CXG motif with a well-preserved

cysteine were identified. While glycine and phenylalanine

can be variable. The glycine, dominant amino acid, was at

the third position of the motif. In these sequences,

asparagine was more dominant. For modeling the proteins,

the Mode base program was used. Among the selected

models, the model with the lowest e-value and the highest

coverage was selected as the best model. Finally, the

quality of the designed models was evaluated using the

ProSA program with energy calculation and Z-score. In all

cases, the energy below zero and the Z-score indicated that

the model was appropriate. All models were in the range

of 3D structures determined by the X-ray method.

Counting the number of alpha helixes and beta pages were

carried out with the Stride Web Interface and were 7-18

and 4-13 respectively. The presence of a glycine in the

interval between the four amino acids before cysteine and

another glycine in the interval between two amino acids

afterward led to the formation of two helices in the 3D

protein structure. It should be noted that the 3D structure

of these seven enzymes was first determined in this study.

Keywords: Amino acids, Motifs, Cytochrome P450,

Entomopathogenic fungus, Modelling

Evaluation of Hsa-miR-940 expression in

tumoral and marginal tissues of the patients

with breast cancer

Ali Abedinzadeh Geshlagi 1, 2, Mohammad Khalaj-Kondori2*, Raheleh

Majdani1, Mohammad Ali Hosseinpour Feizi2 1 Department of Cellular and Molecular Biology, Faculty of Basic

Science, University of Maragheh, Maragheh, Iran. 2 Department of Animal Biology, Faculty of Natural Science, University of Tabriz, Tabriz, Iran.


Breast cancer is one of the most common cancers and the

second cause of cancer-related death among women.

During the recent years, microRNA (miRNA) has become

increasingly recognized as an important regulator of

cancer cell biology. miRNAs are small non-coding RNAs

with 18–22 nucleotides that bind the 3′UTR of target

mRNAs to reduce their stability and/or translation. The

expression of Has-miR-940 has been shown to play an

important role in various cancers. The present study aimed

at evaluation of Hsa-miR-940 expression in breast cancer.

The tumoral and marginal tissues of the breast cancer

patients collected and stored at -80 C until use. Total RNA

was isolated from the breast cancer tissue using RNX-Plus

according to the manufacturer’s protocol. Then cDNA was

synthesized by PrimeScriptTM RT reagent Kit (TaKaRa).

Has-miR-940 expression was analyzed by real-time PCR.

U6 was used as an internal control gene. Primary data

from Real-time PCR indicated downregulation of Has-

miR-940 in tumoral tissues in comparison with the

marginal tissues.

Keyword: Breast cancer, Has-miR-940, miRNA



78

Expression analysis of Long non-coding RNA

SNHG17 in breast cancer

Saeid Amirian-ghatar, Mohammad Khalaj-kondori*, Mina Zafarpiran,

Mohammadali Hosseinporfeizi Department of Genetics, Animal Biology Group, Faculty of Natural

Science, University of Tabriz, Tabriz, Iran.

*corresponding author; [email protected]

Breast cancer is a common type of cancer among women

worldwide. In recent decades despite of impressive

advances in diagnostic and therapeutic strategies, mortality

of breast cancer is still significant. Studies demonstrated

that Long non-coding RNAs (lncRNAs) as an important

group of non-coding RNAs play key roles in development

and progression of different cancers, including breast

cancer. SNHG17 (Small nucleolar RNA host gene 17) is a

novel lncRNA that have been reported in some cancers

such colorectal cancer. This study aimed to evaluate the

expression of SNHG17 in breast cancer. Breast tumor

tissues and their non-tumoral marginal samples were

obtained from 30 patients with breast cancer from the

Nejat hospital of Tabriz between 2014 and

2015.Demographical data like age, grading of the samples

and were collected. Total RNA was purified with RNX-

Plus and cDNA was synthesized by PrimeScript™ RT

reagent Kit )TaKaRa(, then expression of lncRNA

SNHG17 was quantified using qRT-PCR. GAPDH was

used as internal control gene. The qRT-PCR results

indicated that SNHG17 expression in tumor tissues was

rather lower than to margin tissues. However, SNHG17

expression did not show correlation with demographics of

patients.

Keywords: Breast cancer, lncRNA, SNHG17, qRT-PCR

Evaluation of methylation and expression of

miR-96 in tumor tissue versus margin in

patients with breast cancer

Samaneh Heydarzadeh1, Mohammad reza Alivand2, Farrokh Karimi1* 1 Department Genetic, Faculty of Basic Sciences, University of maragheh, Iran 2 Department of Medical Genetics, Faculty of Medicine, Tabriz

University of Medical Science * Corresponding author: [email protected]

Breast cancer and its metastatic progression are mainly

driven by changes in epithelial to mesenchymal state, a

phenomenon that relies on specific transcription agents

and miRNAs. MicroRNAs, as key regulators of the

expression of genes after transcription, can participate in

the control of physiologic and pathologic cellular

processes. Given that a microRNA can act as an oncogene

or tumor suppressor and can also control the expression of

several genes, the change in the methylation pattern of the

promoter region of the gene encoding it in the microRNA

and as a result of its expression change can lead to various

cancers. Several microRNAs have been studied in a

variety of cancers. This study examines miR-96, which is

related to the conserved miR cluster of 183/182/96. In this

study, the tissue samples extracted from the Tumor tissue

and its margin of 100 patients with breast cancer were

crushed using liquid nitrogen and the DNA samples were

extracted manually and the RNA sample was extracted

using a kit. Then, cDNA of this samples were synthesized

and performed RT-PCR and the results were analyzed,

there was a significant relationship between the expression

and the methylation of this microRNA in breast cancer.

The results showed that miR-96 had a significant

upregulation in tumor tissue in breast cancer due to

hypomethylation.

Keywords: Cancer, Methylation, Expression, microRNA


79

Evaluation of methylation and expression of

miR-196b in tumor tissue versus margin

patients with breast cancer

Samaneh Heydarzadeh1, Farrokh Karimi1, Mohammad reza Alivand2*

1 Department Genetic, Faculty of Basic Sciences, University of maragheh, Iran 2 Department of Medical Genetics, Faculty of Medicine, Tabriz

University of Medical Science, Iran * Corresponding author: [email protected]

There are many indications that the change in the

methylation pattern of the promoter region of the miRs

encoding genes is the most epigenetic change observed in

cancers. Previous studies have shown that miR-196b act as

an oncogene or tumor suppressorin various cancers. The

aim of this study was to investigate the role of this miR in

breast cancer as one of the most common cancers among

women and the effect of methylone on its expression. In

this research, by evaluating the expression levels of miR-

196b extracted from 100 tumor tissue and tumor margins

by Real-time PCR, and evaluating the methylation of the

mir-196b gene promoter by treating DNA samples with

sodium bisulphate and performing MS-PCR and

comparing the methylation state of this miR with Its

expression in the tumor tissues relative to its margins was

shown that have a significant relationship between the

aberrant methylation of the promoter region and its

expression level. As a result, its alteration of expression

patterns could be involved in the process of invasion of

breast cancer into secondary tissues and convert this miR

to a Diagnostic biomarker in breast cancer and as one of

the therapeutic goals of this cancer, Should be considered.

Keywords: Expression, Methylation, microRNA, Cancer,

Invasion

Overexpression of α-Synuclein inSHSY5Y cell

to generate a model for Parkinson’s disease

Faezeh Dehghani Esmatabad, Dina Morshedi*, Mehdi Eskandarian, Sina

Mehrpooyan, Farhang Aliakbari Bioprocess Engineering Research group, Institute of Industrial and

Environmental Biotechnology, National Institute of Genetic Engineering

and Biotechnology, Tehran, Iran. * Corresponding author: [email protected]

The aggregation of α-synuclein (α-Syn), a protein found at

high concentration in the brain, has been shown to be

involved in Parkinson’s disease (PD) and other α-

synucleinopathies. To unravel the complex pathological

processhappening in PD, developing the controllable

cellular models is welcome worldwide including numerous

α-Syn based models. One of the events that cause PD is

the overexpression of α-Syn and so making a cell model

using the high expression of the heterologous protein could

be useful in the studies related to the mechanism of PD as

well as related pharmaceutical investigation. SH-SY5Y

cells possess a complete dopaminergic system that uses

widely for modeling PD. Herein, we used a lentiviral

vector to overexpress α-Syn in SH-SY5Y cells as a model

of PD due to it's high-level and long-term transgene

expression. Accordingly, αSN cDNA complemented with

Kozak sequence was cloned into the pLEX-JRed-

TurboGFP lentiviral plasmid (Stem Cell Technology

Research Center). The obtained construct along with

packaging and envelope plasmids were transfected to the

HEK293T cell line. The supernatant of the cell culture

medium was collected and concentrated using PEG6000.

HEK293T cells were infected with concentrated viruses

and the viral titer was determined. SHSY5Y cells

overexpressing α-Syn were prepared using infection with

obtained lentiviruses. This cell line can be used for more

pharmaceutical investigation and more genetic mechanism

of PD.

Keywords: Alpha-synuclein, Parkinson disease, SHSY5Y

cell, HEK293T

80

Sequencing of the acetolactate synthase gene

in the milk thistle, Silybum marianum (L.)

Gaertn

Negin Bermeh*, Mohammad Farkhari, Elham Elahifard

Department of Plant Production and Genetics, Khuzestan Agricultural Sciences, and Natural Resources University, Iran


Milk Thistle (Silybummarianum (L.) Gaertn) is a common

weed species for wheat, barley and corn fields in, Iran.

Acetolactate synthase (ALS) is a chloroplastic enzyme that

encoded by a nuclear gene. ALS catalyzes the first step in

the biosynthesis of branched-chain amino acids.

Sulfonylureas herbicidesinhibit the growth of plants by

blocking ALS enzyme activity Resistance to this family of

herbicides has been observed in some of the weed species

due to a mutation in the ALS gene. Therefore, ALS gene

sequencingsurveyof herbicide-resistant biotypes is

necessary for detecting unknown mutations. In this study,

for the first time sequencing of the ALS gene in Milk

Thistle was done. To this end, were designed a total of five

degenerate primer pairs based on the sequence of this gene

in other species of the Asteraceae family, so that neighbor

produced amplicons, overlapped together about 200-300

bp. Then, they were amplified by polymerase chain

reaction (genomic DNA used as a template). Sequencing

of PCR products was done based Sanger method. Then,

the sequenced parts were assembled using their

overlapping sections. A total of 1921 bp of the gene was

sequenced in Milk Thistle, which covered 88% of the ALS

gene sequence in Arabidopsis (2009 bp) with a similarity

of 74%.

Keywords: Mutation, Herbicide resistance, Sulfonylureas

herbicides, Silybum marianum L.

DSCAM-AS1 lncRNA upregulates in ductal

breast cancer tumoral tissues

Mahsa Tarighi1, Mohammad Khalaj-Kondori2*, Mohammad Reza

Mashayekhi1 1 Department of Genetics, Faculty of Basic Science, Islamic Azad

University, Tabriz Branch, Tabriz, Iran 2 Department of Animal Biology, Faculty of Natural Science, University of Tabriz, Tabriz, Iran


Breast cancer as the most lethal malignancies among

women in the world is a heterogeneous disease and its

clinical management is difficult. So, determining

biomarkers for diagnosis and prognosis of this cancer is

important. Long non-coding RNAs (lncRNAs) are

transcripts with more than 200bp in length that do not code

any protein. They are an important category of RNAs

involved in many important biological processes including

cancer. One of the most novel lncRNAs that have been

reported in cancer progression is DSCAM-AS1. Here we

aimed at evaluation of the DSCAM-AS1 expression in

ductal breast cancer. To test this hypothesis, tumor

samples from 25 patients with breast tumor were collected

(breast tumor tissues and their marginal normal ones).

Total RNA was extracted using RNX-plus and cDNA was

synthesized by PrimeScriptTM

RT reagent kit (TaKaRa).

Level of the DSCAM-AS1 lncRNA expression was

measured using Real-Time PCR and the results compared

between the two groups. GAPDH was used as an internal

control gene. We observed increased expression of the

DSCAM-AS1 in tumor tissues compared with marginal

ones (fold change: 5, p-value ≤ 0.05). In conclusion, this

study further implicates DSCAM-AS1 lncRNA as a strong

genetic candidate in breast cancer. However, more

specimens might be analyzed.

Keywords: Ductal breast cancer, lncRNA, DSCAM-AS1,

qRT-PCR


81

Identification of the key genes/proteins in

hepatitis B virus and hepatocellular

carcinoma via functional clusters in a protein-

protein interaction network

Mahboubeh Mehmankhah1, Syed Naqui Kazim2*, Zarrin Minuchehr3 1 Center of Interdisciplinary Research in Basic Sciences, Jamia Millia Islamia, India and Systems Biotechnology Department, National Institute

of Genetic Engineering and Biotechnology, Tehran, Iran 2 Tehran- Systems Biotechnology Department, National Institute of Genetic Engineering and Biotechnology, Tehran, Iran 3 Center for Interdisciplinary Research in Basic Sciences, Jamia Millia

Islamia, India


Hepatitis B virus (HBV) has been known as a major cause

of hepatocellular carcinoma (HCC). The aim of the current

study was to identify the hub genes/proteins which are

common between hepatitis B virus (HBV) and

hepatocellular carcinoma (HCC) and investigate the

molecular mechanisms of those using Protein-Protein

network. According to previous studies, we gathered 146

HBV-targeted human protein (HHBV) and 666 unique

human proteins that interact with HCC (HHCC). 75 genes

were considered as a seed for making the PPI network,

which were common between HBV and HCC, using

Cytoscape3.4. Subsequently, ClusterMaker app was

applied to conduct a cluster analysis of the constructed

network. Finally, the Cytoscape 2.1 app was also

performed for each cluster and according to reports of

centiscape 2.1 the hub genes/proteins were extracted. Then

Gene ontology (GO) enrichment was applied to our

derived data. Analysis of the PPI networks introduced 7

hub proteins, namely AKT1, SKP2, PPARG, CD81,

CCNB1, STAT1, CCND1, CDKN2A which were

presented in 7 PPI clusters. In view of this, the screened

key genes/proteins may have the potential to become a

candidate for diagnosing and treatment of HCC

transformed from HBV.

Keywords: Hepatocellular carcinoma, Hepatitis B virus,

PPI network, Hub genes/proteins

Evaluating and designing contraceptive

vaccine and recombinant fusion protein based

on IZUMO, SPRASA and PH-20 epitopes

Behnam Mortazavi1*, Najaf Allahyari Fard2. Farid Heidari1 1 Department Animal and Marin Biotechnology, National Institute of Genetic Engineering and Biotechnology (NIGEB) 2 Department of Systemms Biotechnology, National Institute of Genetic

Engineering and Biotechnology (NIGEB) * Corresponding author: [email protected]

Contraceptive vaccines can be used as a valuable and

alternative method to providePurposeful infertility in

humans and animals. There are several targets for

producing these vaccines, such as the use of superficial

sperm proteins. We designed a novel chimeric protein that

contains IZUMO, SPRASA and PH-20 epitopes of

superficial sperm protein. In this study, IZUMO, SPRASA

and PH-20 isoforms were investigated and then in high

conservation regions and by using various tools including

IMED, IEDB and EMBOSS as well as based on the

structure and sequence techniques and various indices such

as specificity, availability, weight and length of epitopes,

antigenicity intensity, topological studies and evaluation of

related-plots to determine the IZUMO, SPRASA and PH-

20 protein's epitope. Protein epitopes selected based on

mentioned criteria and Then epitopes joint together by

using linkers. The final structure was simulated by

GROMACS and Force field of AMBER software. The

final protein was introduced as a recombinant protein for

the development of contraceptive vaccine. The

recombinant protein has a higher ability to induce immune

system instead of using mentioned proteins, because we

make a protein consist of 3 epitopes with high antigenicity

plots. Therefore it can induce immune system 3 times

more than normal proteins.

Keywords: Contraceptive vaccine, Fusion protein,

Epitope, Sperm superficial protein, Bioinformatics

82

DNA methylation analysis of the pro-

inflammatory IL6 gene in Type 2

Diabetespatients

Naeimeh Roshanzamir*, Vahideh Hasanzadeh

Department of Cellular&Molecular Biology, Faculty of Biology, University of Tehran


Type 2 diabetes (T2D) is a metabolic disorder,

characterized by progressive dysfunction of pancreatic β-

cells and insulin resistance, resulting from impaired insulin

signaling. The prevalence of T2D is rising sharply in

association with increases in obesity. Obesity-induced

T2D is recognized as an auto-inflammatory disease. A

state of chronic low-grade inflammation promoted by

obesity, which is reflected by an increased production of

pro-inflammatory cytokines, may contribute to the

development of T2D. Many studies now converge to show

that several cytokines, such as IL-1β and IL-6 contribute to

the pathology and physiology of T2D through their

interaction with insulin signaling pathways and β-cell

function.Type 2 diabetes is the result of interaction

between epigenetic factors and a strong hereditary

component. Epigenetics has been defined as “the study of

changes in gene function that are heritable and that do not

entail a change in DNA sequence”. The aim of this case-

control study, was to investigate the changes in

methylation pattern of IL-6 in peripheral blood

mononuclear cells from individuals with normal (n = 15),

moderately high (n = 15), and high (n =15) blood glucose

levels, using bisulfite sequencing. Considering IL6 dual

pre- and anti-inflammation behavior in the inflammation,

there was not a significant change in methylation pattern

of IL-6 in individuals with diabetes and pre-diabetes

compared with controls.

Keyword: Methylation, Diabetes, Inflammation

DNA methylation analysis of pro-

inflammatory genes in patients affected with

type 2 diabetes

Naeimeh Roshanzamir*, Vahideh Hasanzadeh

Department Cellullar& Molecular Faculty of Biology University of Tehran, Iran


According to epidemiological studies, around 1.5 million

individuals in Iran are affectedby diabetes and between

14.5 to 25.5 % of the population suffer from impaired

glucose tolerance. Type 2 diabetes (T2D) is a metabolic

disorder characterized by insulin resistance and decreased

the production of insulin in pancreatic β-cells, which

consequently lead to reduced glucose transport into

adipose tissue, the liver, and muscle cells. Obesity is

strongly associated with the prevalence of T2D. It is

currently well-accepted that obesity induces a state of

chronic low-grade inflammation, which is reflected by an

increased production of pro-inflammatory cytokines.

Increasing data suggest that numerous cytokines, such as

IL-1β and IL-6, could contribute to the development of

T2D through their detrimental effect on the insulin

signaling pathway and β-cell function. In the present study,

we used bisulfite conversion of DNA and quantitative

techniques to examine the changes in DNA methylation

patterns of regulatory regions in inflammatory genes

namely IL1β in three groups with different plasma glucose

levels. Statistical analysis was performed by using

"Prism7" and "Plasmid editor". Compared with control

subjects, T2D patients, and pre-diabetic showed

significantly lower levels of DNA methylation in IL1β.

Inflammation. However, no significant change was

observed in methylation in comparison with pre-diabetic

diabetics with diabetes. Based on these results,

methylation pattern changes could be used to evaluate the

progression of type 2 diabetes.

Keyword: Diabetes, Epigenetics, Pro-inflammatory genes,

Methylation

83

Isolation and identification of the c-type

lysozyme-encoding gene from Salmo trutta

caspius

Mahboubeh Kaviani, Mahmoudreza Aghamaali*, S. Shirin Shahangian



The innate immune system of fish is considered to be the

first line of defense against a broad spectrum of pathogens.

Lysozymes are key proteins of the innate immune system

against bacterial infection. Non-specific antimicrobial

agents, such as lysozyme, may be more important in fish

comparing tomammalsbecause fish apparently has a less

developed specific immune system. Salmonid fishes have

long been of great interest due to the commercial

importance as model systems for addressing a wide range

of evolutionary and ecological question. In this study, we

report the isolation, amplification, and characterization of

chicken-type (c-type) lysozyme gene from Salmo trutta

caspius. After total RNA extraction from the apical

compartment of the fish kidney using TRIzol reagent,

complementary DNA (cDNA) was synthesized by cDNA

Synthesis kit. The cDNA was used as a template for the

PCR using specific primers that annealed to the conserved

regions of the lysozyme gene from another genus of

Salmonidae family. Analysis of the sequence of amplified

gene revealed that the cDNA contains an open reading

frame (ORF) of 432bp, encoding 143 amino acid, with

97% identity toLysozyme C of Rainbow

trout(Oncorhynchusmykiss).

Keywords: Salmo trutta caspius, Immune system,

Lysozyme C, cDNA synthesis, Gene amplification

Resveratrol and breast cancer: the survival of

cancer cells and expression of caspase gene 3

Ehsan Ghodrati Shatori1*, S. Kazem Sabbagh2, Narges Nikonahad1 1 Yazd University of Art and Science, Iran 2 Department of Biology, Yazd University, Iran


Breast cancer, the most common type of cancer, is a major

health concern, and after lung cancer is the second leading

cause of death and mortality among women. Regarding the

side effects of chemotherapy, there has been a growing

interest in using natural drug sources to treat this disease.

In this study, the effect of Red Vint Resveratrol on the

survival of MCF-7 breast cancer cells and the expression

of the Caspase 3 gene was investigated. For this purpose,

the cells were treated with different concentrations of the

drug (25, 50, 75, 100, 150, ppm) for 24, 48 and 72 hours

and stored under sterile conditions and harvested at the

above time intervals. The results indicated that with

increasing concentration and time, the vitality of the cells

had a significant decrease compared to the control samples

and by a 50 ppm treatment 50% of the cells (IC50) were

loosed. The results of gene expression analysis showed

that caspase 3 expression in 24 hours increased

significantly compared to the control group as well as

other time intervals (48 and 72 hours). According to the

obtained data, it can be concluded that resveratrol or

similar natural drug compounds can be used as an

alternative to breast cancer treatment. Keywords: Breast cancer, Resveratrol, IC50, Caspase 3,

Gene expression


84

Improvement the effect of green synthesized

nano-oxali palladium in comparison with

oxali palladiumagainst human colon cancer

cell line HCT116

Nasim Golestannejad1, Adeleh Divsalar1*, Saeed Irian1, Arefeh

Seyedarabi2 1 Department of Cellular& Molecular Sciences, Faculty of Biological

Sciences, Kharazmi University, Tehran, Iran 2 Institute of Biochemistry and Biophysics (IBB), Tehran University, Tehran, Iran


Previous reports showed the anti-cancer activity of oxali-

Palladium and turmeric extract in colon cancer treatment.

In this study, we investigated the cytotoxic effects of green

synthesized nano-oxali palladium using turmeric extract by

green chemistry method in comparison with free oxali-

palladium against human colon cancer cell line of

HCT116. At first, the nano-oxali palladium had to be

synthesized by green chemistry method, then the

cytotoxicity and antiproliferative activities of nano-oxali

palladium, oxali palladium, and turmeric extract were

examined by 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyl

tetrazolium bromide (MTT) assay after 24 and 48 hours

incubation times. To synthesize of nano-oxali palladium,

the alcoholic extract of turmeric incubated with an oxali-

palladium solution at 500 C for 24 h in shaker incubator

then physical and chemical properties of nano particle such

as size, shape and etc.were studied. The 50% cytotoxic

concentration (Cc50) of the nano-oxali palladium was

calculated 78 and 57 (µM) after 24 and 48 h incubation

times whereas this value was evaluated 600 and 433 µM

for free oxali palladium after 24 and 48 h incubation times.

Respectively, 45 and 32 (mg/ml) of turmeric extract after

24 and 48 h incubation times were induced death in 50%

of HCT116 cell line. The results show that green

synthesized nano-oxali palladium using turmeric extract

has better or more cytotoxic effect on HCT116 colon

cancer cell line to induce death in 50% of cells after

different incubation times of 24 and 48 hours. Also, the

above results illustrate that the anti-cancer activity of

turmeric extract which is used as the cover of nano-

oxalipalladium is amplifying in nano form and increases

the cytotoxicity of this compound.

Keywords: Colon cancer, Oxali Palladium, Turmeric

extract, Green chemistry

Design of diazo dyes based on 2, 6-diamino-4-

chloropyrimidine compound and the analysis

of their interaction with tyrosinase using

molecular docking method

Mahdieh Farvandi1*, Hossein Ghafouri1,Asadollah Mohammadi2, Mostafa

Shourian1 1 Department of Biology, Faculty of Science, University of Guilan, Iran 2 Department of Chemistry, Faculty of Science, University of Guilan, Iran


Tyrosinase is a copper-containing enzyme which belongs

to the oxidasesuperfamily protein. This enzyme catalyzes

the main reaction of melanin biosynthesis; however, its

abnormal accumulation is responsible for

hyperpigmentation related disorders like senile lentigines,

freckles, melasma and other forms of melanin

hyperpigmentation which causes serious esthetic problems.

The reactions catalyzed by this enzyme, the hydroxylation

of a monophenol and the conversion of an ο-diphenol to

the corresponding o-quinone lead to melanin production

which plays a vital protective role against skin photo-

carcinogenesis, and hence knowledge of tyrosinase

catalytic mechanisms and regulation may have medical,

cosmetic and agriculture application.Numerous efforts

have so far been made to develop new tyrosinase inhibitors

around the world and various synthetic chemical

compounds have been nominated. Two novel compounds

based on 2 ,6-diamino-4-chloropyrimidine were

synthesized and evaluated as tyrosinase inhibitors. In order

to know the binding of the synthesized compounds,

molecular docking studies of the compound were carried

out against tyrosinase enzyme and make it possible to gain

a better understanding of the tyrosinase inhibition

mechanism. The chemical structure of all compounds was

designed using the chemdrawprogramand then

subjectedinto Hyperchem software for energy

minimization.Themushroom tyrosinase (PDBID 2Y9X)

was dockedby Autodock 4.2 program with synthesized

compounds. The structure of the compounds was

confirmed by FT-IR, H NMR, and C NMR spectroscopic

techniques. We simulation the docking between tyrosinase

and compounds, and results suggested that these

compounds inhibit tyrosinase activity, and this result

confirmed by tyrosinase assay in experimental methods.

Keywords: Tyrosinase, Molecular docking, Inhibitors,

2 ,6-diamino-4-chloropyrimidine

http://www.pubfacts.com/author/Saeed+Irian


85

Investigation of the gene expression profiling

of photoreceptors in separated reproductive

and somatic cells in multicellular green algae

Volvox carteri at low intensity of UV-B

radiation

Soulmaz Ekhtari1*, Jafar Razeghi1, Karim Hasanpur2, Arash

Kianianmomeni3, Ali Movafeghi1

1 Department of Plant Biology, Faculty of Natural Sciences, University of

Tabriz, Tabriz, Iran 2 Department of Animal Science, Faculty of Agriculture, University of Tabriz, Tabriz, Iran 3 Department of Cellular and Developmental Biology of Plants, Faculty

of Natural Sciences, University of Bielefeld, Bielefeld, Germany * Corresponding author: [email protected]

Light is an important source of energy for the

photosynthetic organisms. Volvox carteriis a simple

multicellular green alga with many features that

recommend it as a lower eukaryote model organism for

studying the development of photoreception. In the current

work, the effect of UV-B radiation (0.056 mw.cm-2

) was

studied on gene expression of 13 photoreceptors using

RNA-seq data. These photoreceptors are required for

accurate light-monitoring and adaption of its physiological

activities to environmental changes. According to our

results, under the low intensity of UV-B radiation, the

photoreceptors were differentially expressed neither in

reproductive cells nor in somatic cells as compared to their

corresponding control groups. However, comparing the

transcriptome of somatic cells with reproductive cells,

revealed that Phot, CRYp, and ChR1-2, HKR1-4 and Vop

(VR1) photoreceptors exhibited a cell-type specific

expression pattern while photoreceptors such as UVR8,

CRYd1-2, and CRYa were differentially expressed.

However, it seems that due to significantly transcript

accumulations in somatic cells, likely UV-B may

indirectly affect gene transcription in this organism.

Somatic cells differ in reproductive cells in function,

biochemical composition, size, and structure. Therefore,

they have different energy balance and possess their own

circadian rhythms and metabolic profiling. Moreover,

depends on their localization in an organism, they are

subjected to various light intensities. The cell-type specific

transcriptome pattern shows the different nature of somatic

and reproductive cells, which is the first step in the

differentiation and initial division of work between cells.

Keywords: Cell types, Light signaling, Photoreceptor,

UV-B radiation, Volvox carteri

A comparative transcriptome analysis of two

cell-types of colonial green alga Volvox carteri

Soulmaz Ekhtari1*, Karim Hasanpur2, Jafar Razeghi1, Arash

Kianianmomeni3, Ali Movafeghi1 1 Department of Plant Biology- Faculty of Natural Sciences, University of

Tabriz, Tabriz, Iran 2 Department of Animal Science- Faculty of Agriculture, University of Tabriz, Tabriz, Iran 3 Department of Cellular and Developmental Biology of Plants- Faculty

of Natural Sciences, University of Bielefeld, Bielefeld, Germany * Corresponding author: [email protected]

The evolutionary origin of some of the multicellular

organisms is not yet comprehensively studied. Germ-soma

differentiation is an obvious characteristic of complex

multicellular organisms. The multicellular green alga,

Volvox carteri composed of only 2000–4000 terminally

differentiated somatic cells, which build a monolayer at

the surface of a spheroid, and around 16 much larger

reproductive cells within the surface. Finding out how

unicellular organisms can develop in to multicellular

organisms over the course of evolution is a central issue in

biological research. V. carteri is a simple organism which

provides a unique opportunity to study the molecular

mechanisms of transmission from unicellularity to

multicellularity and to discover universal rules of cellular

differentiation in eukaryote. Here, the RNA-seq approach

was employed to investigate transcriptome profiling of two

separated cell types of V. carteri. Our results revealed that,

almost half of the V. carteri genes (11783 genes out of

21000 genes) were differentially expressed between the

two cell-types. This strongly suggested different functions

for genes of each cell type and demonstrated a more

complex process of differentiation. Almost 30% of

differentially expressed genes were from loci without any

annotation and can be considered as novel genes that need

to be identified. Therefore, a comprehensive comparative

analysis is required to identify the determined cell type

function of each differentially expressed gene and to solve

their rules in differentiation process.

Keywords: Cell types, Cellular differentiation, Volvox

carteri, RNA-seq, Transcriptomics

86

Molecular docking of 2,4,6-

triaminopyrimidine derivatives as tyrosinase

inhibitors

S. Shohreh Mirmortazavi1*, Hossein Ghafouri1, Asadollah Mohammadi2,

Mostafa Shourian1 1 Department of Biology, Faculty of Science, University of Guilan, Iran 2 Department of chemistry, Faculty of Science, University of Guilan, Iran


Tyrosinase is a copper-containing monooxygenase that

catalyzes the oxidation of tyrosine and produces melanin

in melanocytic cells. The excessive production of melanin

leads to hyperpigmentation disorders, wrinkling, and

melasma and skin cancer. Inhibition of melanin synthesis

is being considered as a valid therapeutic strategy for the

treatment of advanced melanotic melanomas and other

pigmentation disorders. Studies on the synthetic

compounds with inhibitory potential have resulted in the

discovery of some effective agents. In this study, two

novel 2, 4, 6-triaminopyrimidine derivatives were

synthesized and evaluated as tyrosinase inhibitors. The

mushroom tyrosinase (PDBID 2Y9X) was docked with

synthesized compound and the binding energy was

calculated. The chemical structure of compounds was

designed using the ChemDraw program. It then subjected

to HyperChem software for energy minimization. Docking

study was performed by Autodock 4.2 program. The

docking between tyrosinase and synthetic compounds

suggest that these compounds significantly inhibit

tyrosinase activity. The structures of the compounds were

confirmed by FT-IR, C NMR, and H NMR.

Keywords: 2, 4, 6-triaminopyrimidine, Tyrosinase,

Inhibitor, Molecular docking

Association between ADSL gene (rs 3788577)

polymorphism and breast cancer in East

Azarbaijan population

Parisa Malekpour1, Mohamad Reza Alivand2, Hossein Soltanzadeh1*

1 Bonab Islamic Azad University 2 Tabriz University of Medical Sciences


Breast cancer is one of the most common types of cancer

that causes many deaths among women and men every

year. The aim of this study was to investigate the

association of ADSL-dependent rs3788577 polymorphism

with breast cancer in the East Azarbaijan population.In this

study, 100 blood samples of patients with breast cancer

and 100 blood samples from healthy persons as control

group were selected. Then DNA was extracted from all

samples using kit of DNA extraction according to the

protocol. In the next step, the specimens were amplified by

PCR and electrophoresis was performed again. Ultimately,

PCR products were treated with the restriction enzyme

Hpa II * (BsiS I) (Msp I) and electrophoresed on the

agarose gel and the polymorphism was observed as bands.

Data were analyzed by SPSS software Version 21 and

evaluated by descriptive and chi-square test. The

percentage of allele A in healthy persons and patients was

85.5% and 41.5% respectively, and G allele in healthy

persons and patients was 14.5% and 58.5%, respectively.

The results of this study indicate that the G allele in

patients is elevated by 44% in comparison to healthy

persons. There is probably a correlation between the

increase in the G allele (44%) and the incidence of breast

cancer. Therefore, the relationship between rs3788577

polymorphism and breast cancer can be revealed through

more detailed studies.

Keywords: Breast Cancer, Polymorphism, rs3788577,

ADSL

http://www.bonabiau.ac.ir/en/default.aspx

https://portal-en.tbzmed.ac.ir/


87

Investigation ofinteraction of a novel synthetic

acridine-derived inhibitor with

acetylcholinesterase enzyme by molecular

docking

Maryam Hatami1, Safa Lotfi*, Elham Rezvannejad, Mojtaba Mortazavi

Department of Biotechnology, Institute of Science and High Technology and Environmental Sciences, Graduate University of Advanced

Technology, Kerman, Iran.

Corresponding author: [email protected]

Acetylcholinesterase enzyme is a serine hydrolase, most

commonly located at the site of neuromuscular attachment

and cholinergic brain synapses, which terminates synaptic

transmission through hydrolysis of acetylcholine

neurotransmitter. In cholinergic hypothesis which is one

of the proposed hypotheses about the cause of Alzheimer's

disease, the Alzheimer-related dementia is attributed to the

reduced level of acetylcholine neurotransmitter in the

cortex and other regions of the brain. Therefore, the

administration of acetylcholinesterase inhibitors serves as

one of the powerful Alzheimer therapeutic strategies. In

this enzyme, the residues involved in substrate binding and

catalytic activity are located at the depth of a narrow

hydroponic gorge located on the surface of the enzyme.

The entrance of this gorge is called the peripheral anionic

site. In the present work, the interaction of a synthetic

novel acridine derivative with the acetylcholinesterase

enzyme has been investigated using molecular docking

method. It should be noted that based on the previous

experimental work, the high ability of this compound to

inhibit acetylcholinesterase enzyme had been proven. In

order to conduct this research, PDB file of the enzyme was

downloaded from PDB site and PDB file of the inhibitor

was prepared using ChemDraw software. Then the

required molecular docking inputs were provided by

AutoDockTools software. Finally, the molecular docking

was performed using AutoDock Vina software and the

results were analyzed using PyMol, Chimera, and LigPlot

software. The results of this study indicate that this

compound is longitudinally introduced into the

hydrophobic catalytic gorge, but does not penetrate to the

depth of the gorge. In fact, this inhibitor interacts with the

residues of the peripheral anionic site and thus blocks the

entrance of substrate to the active site. The results obtained

from this study could be used to design new effective

drugs for the treatment of Alzheimer's disease.

Keywords: Acetylcholinesteraseinhibitor, Acetylcholine,

Alzheimer's disease, Acridine derivative, Molecular

docking

Investigating the conformity of the second law

of Chargaff on variable-length homopolymer

fragments in the human genome

Reza Sotoudeh1, Javad Zahiri2, Hossein Naderi-Manesh* 1 Department of Nanobiotechnology/Biophysics, Faculty of Biological Science, Tarbiat Modares University, Tehran, Iran 2 Bioinformatics and Computational Omics Lab (BioCOOL), Department

of Biophysics, Faculty of Biological Science, Tarbiat Modares University, Tehran, Iran


Chargaff studies on thefour DNAnucleotides showed that

the frequency of A and C is equal to T and G respectively

in an organism's genome. This equality in the double-

stranded DNA known as Chargaff's first law, which is the

result of the formation of The Watson-Creek pairing in the

double-helix structure of DNA.The Chargaff's second rule

states that the first rule also holds in ssDNA. These

tworulescover all the double-stranded genomesexcept

organelle genomes1. In this study, we have analyzed the

human genome to investigate the evidence of a generalized

form of the Chargoff's second rule. Interestingly enough,

results reveal that the frequency of the A and C

homopolymers is equal to the frequency of T and G

homopolymers, respectively.

Keywords: Chargaff's first law, Chargaff's second law,

Homopolymer, Chromosome, Watson-Creek pairing



88

Study of MFN2gene expression in tissue

samples of patients with breast cancer

Seyede Saramohsenizade1, Farnoosh Khosrobakhsh1 1 Department of Biological Sciences, Faculty of Sciences, University of Kurdistan


Cancer is a disease characterized by inappropriate cell

proliferation, uncontrolled cell cycle, and defect in

apoptosis. Mitochondrial dynamics is involved in cell

cycle and apoptotic mechanisms. Previous studies have

been shown the relation between mitochondrial dynamic

processes and progress of various type of cancer.

Mammals have MFN2 (Mitofusin-2) gene. which is

involved in mitochondrial membrane fusion. The aim of

this study was to determine the expression level of mfn2

gene in tissue samples of patients with breast cancer in

Iran.In this study, breast cancer and non-cancerous breast

tissue were taken from 50 Iranian cancer patients for RNA

extraction, cDNA production, and further investigation of

mfn2 gene expression using Real-time PCR method. The

results of this study showed a significant decrease in mfn2

gene expression in tumor tissues compared to the non-

cancerous breast tissues (p<0.01). The results of this study

are consistent with results from previous studies. This

reduction of mfn2 gene expression, which was reported for

the first time in Iran, could indicate an inhibitory effect of

mfn2 on mTORC2 protein (one of the major proteins

involved in growth signal, metabolism and cell

proliferation), confirming the overgrowth of tumor in

comparison to surrounding non-cancerous breast tissues.

Keywords: Dynamic mitochondrial, Fission, Fusion,

Mitofusin

Association of 87851G>A, LMTK2 nucleotide

transition with benign prostatic hyperplasia:

a case-control study in Mazandaran

population

Fereshteh Jozaghkar1, Abasalt Hosseinzadeh Colagar1*, Emaduddin

Moudi2 1 Department of Molecular and Cell Biology, Faculty of Basic Sciences,

University of Mazandaran, Babolsar, Mazandaran, Iran. 2 Department of Urology, Babol University of Medical Sciences, Babol, Iran.


Recently, biomarkers have been used for diagnosis and

prevention of several types of malignant or benign tumors

such as the prostate. One of these markers is the LMTK2

that codes a transmembrane serine/tyrosine kinase which

plays role in membrane transferring. LMTK2 has been

identified to regulate prostate-specific antigen (PSA) and

vascular endothelial growth factor (VEGF) which are

related to tumorigenesis. Also, downregulation of LMTK2

might contribute to prostate cancer formation. To

investigate the correlation of rs7791463 which is located

on intron 9 of LMTK2 and benign prostatic hyperplasia

(BPH), the Boiling DNA extraction and the PCR-RFLP

methods have been used for the blood sample of 70

patients and 70 normal controls participants. The PCR-

product has been 257 bp which has been cut by NcoI

restriction enzyme in the G allele. The frequencies of

genotypes that obtained in the controls were 22.8% GG,

51.4% AG and 25.7% AA, and in the cases were 12.8%

GG, 61.42% AG and 25.7% AA. Also, the

allelicfrequencies that calculated in controls were 51.4 and

48.5 percent for A and G allele, and in cases were 56.4 and

43.5 percent for A and G allele. Statistical analysis

revealed that the distribution of genotypes for

87851G>Atransition does not show Significant different

(P= 0.36, OR=1.26; 95% CI: 0.76-2.1) in case and control

groups. In conclusion, the present study suggests that

87851G>A transition is not associated with BPH.

Keywords: LMTK2, Benign Prostatic Hyperplasia,

Polymorphism

https://www.ncbi.nlm.nih.gov/projects/sviewer/?id=NG_013375.1&search=NG_013375.1:g.87851G%3eA&v=1:100&content=5




89

Jellyfish venom C-CFTX1-STxB chimeric

antigen subcloning, expression and its

antigenicity assay in laboratory mouse

Mahdi Hosseinzadeh1*, Hossein Honari 2 1 Biological Research Center, Faculty of Basic Sciences, Imam Hussein (pbh) University, Tehran. 2 Biological Research Center, Imam Hussein (pbh) University, Tehran.


Box jellyfish inflicts painful stings and may be life-

threatening. The venom of C. fleckeri contains a variety of

bioactive proteins that are cytolytic, cytotoxic,

inflammatory or lethal. Two of the most abundant proteins

contained in the nematocysts of C. fleckeri; CfTX-1 and

CfTX-2 were difficult to separate using electrophoretic or

chromatographic methodsRecombinant expression

technology may offer an alternative to the isolation of

native C. fleckeri venom protein. The aim of this study is

to undertake novel expression studies of the cytolytic

C.fleckeri toxin C-CfTX1-STxB in E.coli and study its

antigenicity in Syrian mice.cftx1 construct based on

bioinformatics designed and synthetic gene prepared in

plasmid pUC57. 576 bp C-cftx1 cloned with PCR and was

subcloned in a pET28a-stxB expression vector with

BamHI and SalI restriction enzyme sites and transformed

into E.coli BL21 (DE3) competent cells. C-cftx1-stxB

gene expression was artificially induced by IPTG, and

protein purified from the gel. Also, Mice were challenged

by the Rhopilema nomadica's venom. In this experimental

study, C-CfTX1-STxB 822 bp gene was confirmed by

PCR, sequencing and enzymatic analysis. Besides

Recombinant protein was confirmed by SDS-PAGE and

Western blotting. Serum was prepared from immunized

mice after blood sampling, and the produced antibody was

quantitated by ELISA. The results showed that immunized

mice in a challenge after 60 days tolerated 50x LD50 of

jellyfish venom. Considering that recombinant C-CfTX1-

STxB protein doesn't have cardiotoxicity and neurotoxicity

effects on mice, this produced protein can be suggested as

a jellyfish venom vaccine candidate for mice or at a later

stage of a clinical trial for humans.

Keywords: Chironex fleckeri, Jellyfish venom, C-CFTX1-

STxB chimeric antigen, Antigenicity assay

Investigation of the expression of two long

non-coding RNAs (KCNQ1OT1 and

MALAT1) in peripheral blood of patients

with acute myeloid leukemia

Zahra Barati Shourijeh1, Zahra Faghih2*, Mahmoud Vesal1, Amin

Ramezani2, Reza Vojdani3

1 Department of Biochemistry, Shiraz Branch, Islamic Azad University,

Shiraz, Iran 2 Shiraz Institute for Cancer Research, Shiraz University of Medical Sciences, Shiraz, Iran 3 Department of Hematology and Medical Oncology, Shiraz University of

Medical Sciences, Hematology Research Center, Shiraz, Iran


Non-coding RNAs (ncRNAs), as the post-transcriptional

regulators, plays a critical role in many cancers’

development and progression. Long non-coding RNAs

(lnc-RNAs) are a heterogeneous class of ncRNAs with

longer than 200 nucleotides which has been introduced to

have an effective role through the complicated processes

including transcription modulation. MALAT1 and

KCNQ1OT1 are two well-known lncRNAs which have

been reported to have a higher expression level in many

types of cancer. In the present study, we aimed to evaluate

the expression level of these two lnc-RNAs in Acute

Myeloid Leukemia (AML) patients in comparison to

healthy individuals. Thirty-two AML patients, as well as

30 sex and age, matched healthy individuals voluntarily

enrolled in the study. RNA was extracted from whole

blood samples using TRIZOL reagents. Following treating

RNA samples with DNase enzyme, complementary DNA

(cDNA) synthesis was performed using TAKARA reverse

transcriptase kit. The expression level of MALAT1,

KCNQ1OT1, and ACTB (as reference gene) was

measured by SYBR green real-time PCR. Relative

expression was calculated by REST 2009 software. Our

results showed a slight increase in the expression of

KCNQ1OT1 (1.75 fold) in one hand and a minor decrease

in the expression levels of MALAT1 (0.87 fold) on the

other in the AML patients compared to the healthy

individuals, however, the differences were not statistically

significant (p≥0.05). In conclusion, although we could not

find any differences in the expression of KCNQ1OT1 and

MALAT1 between patients and controls, more researches

with larger sample size requirements to reveal the exact

role of this lncRNAs in AML.

Keywords: Long non-coding RNA, Acute myeloid

leukemia, MALAT1, KCNQ1OT1



90

Overexpression of VOPP1 and PIK3C2B

genes in chronic lymphoblastic leukemia

Zahra Barati Shourijeh1*, Mohamad Moghadam2 1 Department of Biochemistry, Shiraz Branch, Islamic Azad University, Shiraz, Iran 2 Hematology Research Center, Shiraz University of Medical Sciences,

Shiraz, Iran * Corresponding author: [email protected]

Chronic lymphocytic B cell leukemia (CLL) is a mature B-

cell neoplasm which is more common in men older than

60. Although some biomarkers such as chromosomal

abnormality, b2microglobulin (b2M), CD38 expression,

high expression of unmutated immunoglobulin heavy

chain variable gene (IGHV) and ZAP-70 have been proved

as a predictor of this disease, lot of unknown factors still to

discover. In this study, we evaluated the expression level

of two genes (VOPP1 and PIK3C2B), which involved in

many cancers, in CLL patients for the first time. VOPP1

(Vesi1AQKJKWcular Overexpressed in cancer

Prosurvival Protein 1) boosts the transcriptional activity of

NFKB1 by helping its nuclear translocation. Also, VOPP1

overexpression has been reported in tumorigenesis and

decreasessusceptibility of apoptosis. PIK3C2B

(phosphatidylinositol-4-phosphate 3-kinase catalytic

subunit type 2 beta) plays a critical role in signaling

pathways involved in cell proliferation, oncogenic

transformation, cell survival, cell migration, and

intracellular protein trafficking. After quality control, total

RNA peripheral blood of at least 58 patients with CLL and

60 age and sex-matched healthy individuals extracted

using TRIZOL. After evaluation of concentration and

integrity of extracted RNA, About 500 ng of the

total RNA reverse transcribed into cDNA. Quantitative

real-time polymerase chain reaction (PCR) was performed

using the SYBR green method to determine the expression

level of each gene. Relative gene expression levels were

calculated against the expression of the housekeeping

gene(ACTB) as internal controlswith the Livak

method.Our results showed a significant higher expression

of VOPP1 and PIK3C2B in CLL patients in comparison

with healthy control group (P≤0.05) which is compatible

with findings in another type of cancers.

Keywords: CLL, VOPP1, PIK3C2B, Overexpression

Expression of recombinant EGFP viasurface

display of ice nucleation protein and TEV

protease cleavage site

Fereshteh Ramezani Khorsand1, Mahdi Zeinoddini2, Ehsan Dehnavi3,

Reza H.Sajedi1*

1 Department of Biochemistry, Faculty of Biological Sciences, Tarbiat

Modares University, Tehran, Iran. 2 Institute of Bioscience and Biotechnology, Malek Ashtar University of Technology, Tehran, Iran. 3 Gene Transfer Pioneers (GTP) Research Group, Incubation center of

Pharmaceutical Technologies, Shahid Beheshti University of Medical Science, Tehran, Iran.


The cell-surface display is the expression of proteinson the

surface of prokaryotic and eukaryotic cells. Several

bacteria have cell surface-anchoring proteins such as ice

nucleation protein (INP) that can be displaying passengers

on the outer membrane. INP is an outer membrane protein,

have N-terminal domain, which is important for linkage to

the outer membrane. Green fluorescent protein (GFP) is a

protein that fluorescence green in the presence of light and

used in a variety of applications to study the organization

and function of living systems. Enhanced GFP (EGFP) is

one of the brightest variants of GFP. In this study, we

expressed a gene construct in the form of InaK-N (a

truncated form of Inak variant of INP)-TEV protease

cleavage site-EGFP on the surface of E. coli BL21 (DE3)

and JM109 (DE3) as host strains.The pallet of induced E.

coli was collected by centrifuging and resuspended in the

TEV protease reaction buffer. After incubation with TEV

protease, a 27 KD EGFP band was observed in SDS–

PAGE. To estimate the level of surface expression

([surface EGFP]/[total EGFP]), we recorded the

fluorescence intensity of EGFP in intact E. coli cell before

and after TEV cleavage reaction and then calculated the

concentration of EGFP with standard plot using purified

cytoplasmic EGFP. The requirement of cell disruption and

the chromatographic process is totally omitted and proteins

prone to accumulation as inclusion bodies and have

disulfide bonds could be successfully expressed using this

strategy.

Keywords: Bacterial surface display; INP; EGFP




91

Comparison of two conventional molecular

methods in detecting jak2v617f mutations in

patients with myeloproliferative neoplasms

Aida Mohamad Amoie1, Hamid Rezanezhad2 1 Department of Zhenetic, faculty of Biological Sciences, Tonekabon Branch, Islamic Azad University, Tonekabon, Iran 2 Department of Hematological Oncology Faculty of Hematology,

university of Oloom pezeshki, Tehran, Iran * Corresponding author: [email protected]

Myeloproliferative neoplasms (MPNs) are a heterogeneous

group of diseases in which a clonal disorder of

hematopoietic stem cells leads to an increase in the level of

production in one or more blood cell lines. A link

determined more between these groups of diseases through

the identification of an acquired mutation tyrosine kinase

JAK2. The purpose of this study is two molecular methods

as: ARMS-PCR and PCR-RFLP in mutation detection of

JAK2V617F.In this study two methods of ARMS-PCR

and PCR-RFLP were conducted whit some changes from

the previous research study. In order to compare of

specificity of these two methods 30 samples were taken

from laboratory centers and the reaction of ARMS-PCR

and also PCR-RFLP was performed on the samples

separately and then the samples were compared whit PCR-

SEQUENCING. Our results showed us that both ARMS-

PCR and RFLP-PCR methods have ability to identify and

recognize for JAK2V617F, but the method of ARMS-PCR

is easier and cheaper than PCR-RFLP. Why the mutation

exists in a small proportion of granulocyte population, it is

required a sensitive methods for its detection. And in

accordance whit the importance of mutation of jak2v617f

in chronic myeloid disorders, the discovery of this

mutation in the diagnosis and prediction of this response is

a useful treatment.

Keywords: MPNs, JAK2V617F, ARMS-PCR and PCR-

RFLP

Study of the differentiation of rat omentum

stem cells to nerve cells using brain tissue

extract of rat

Fateme Zahra Rezanejad Keshteli*, Kazem Parivar

Department of Biology, Faculty of Biology, Islamic Azad University, Tehran North Branch


Stem cells have two typical feature self-renewal and

potential of different cell lineages. Omentum stem cells

could be induced to differentiate into neural cells under

certain condition. The main goal of this present study is to

neural differentiation of OSCs induced by using Rat brain

extract. Omentum stem cells were isolated from

peritoneum and brain extract from neonatal rat brain, then

the cells were co-cultured in DMEM and brain extract.

After 3 passage OSCs were morphologically observed and

processed for RT-PCR. The number of cells neural

morphology increased at 1-2 weeks dramatically. The cells

were labeled by neural markers including Map2 which

assessed by immunocytochemistry. Our finding

demonstrated that OSCs efficiently differentiate into the

neural cell when cultured in brain extract.

Keywords: Omentum, Mesenchymal stem cells, Rat

neonate brain extract, Neural cell differentiation



92

Study of gene Polymorphisms correlated with

allergic rhiniris disease in the northwest of

Iran

Nazaila Valatabar¹¸ Mohhamad hossein Pourfeizi1*, Reza Safaralizadeh1,

Mahnazsadegi Shabestari2 1 Faculty of Natural Science, Tabriz University, Iran 2 Tabriz University of Medical Science, Iran


An allergen is an otherwise harmless substance that causes

an allergic reaction, especially in the spring and summer.

Allergic rhinitis is one of a variety of allergic diseases that

affects the upper respiratory tract, The prevalence of AR

has been estimated to be between 15% and 20% and it is

associated with numerous short-and long-term

complications that impact the patient’s overall life quality,

that contributes to the development of other medical

conditions or health issues, such as asthma, sinusitis,

anosmia, otitis media, nasal polyps, lower airway

infection. There is clear evidence to support the concept

that allergic rhinitisinfluenced by genetic predisposition

and environmental exposure. The purpose of this study

was to identify the key polymorphism of genes for AR. In

this study, two groups, allergic rhinitis patients diagnosed

by an allergy specialist as the case group and non-allergic

rhinitis patients were selected as the control. DNA was

extracted from peripheral blood of groups were evaluated

for the genetic polymorphism aspect by PCR-RFLP and

the results were analyzed with SPSS software.It has been

shown that polymorphisms of candidate genes have been

associated with clinical expression of these diseases in the

northwest population of Iran. This study will help us to

further develop new avenues for genetically oriented

diagnosis and more effective measures of prevention and

intervention. However, more epidemiological studies are

needed to accurately identify genes affecting the

Northwest population of Iran.

Keywords: Allergic rhinitis, Polymorphism, Northwest

population of Iran

Decreasing of viability in H2O2treated of ITPA

down-regulated human umbilical vein

endothelial cells

Seyedeh Maral Marashi1*, Zahra Abedi kichi1, Amir Hossain Ahmadi2,

Mehrdad Behmanesh1 1 Department of Genetics, Faculty of Biological Sciences, Tarbiat

Modares University, Tehran, Iran 2 Department of Biology, Faculty of Basic Sciences, Persian Gulf University, Bushehr, Iran


Human inosine triphosphatase (ITPase), encoded by the

ITPA gene is required for high-fidelity DNA and RNA

replication. ITPA has been identified as a key gene

involved in the removal deaminated nucleotides from the

cellular nucleotide pool, maintains the stability of the

genome. Defects in ITPA can result in inosine

triphosphatase deficiency and accumulation of modified or

damaged bases in genomic DNA or cellular RNAs that is a

major cause of altered genetic information and

mutagenesis.Oxidative deamination is a common chemical

modification that damages DNA and RNA molecules. Free

radicals of oxygen and nitrogen produced by oxidative

stress in cells contribute to cell dysfunction via the

apoptotic induction of endothelial cells (ECs). This study

was focused on investigating the survival of stable ITPA

down-regulated HUVEC compared to normal HUVEC in

the presence of H2O2. To evaluate the cell viability assay,

we used the 3-(4, 5-dimethylthiazol-2-yl) -2, 5-

diphenyltetrazolium bromide (MTT) method. Briefly,

1x104 cells were incubated in a 96-well plate in the

presence of various concentrations of H2O2 for 12-24

hours to determine the effect on endothelial cell

proliferation. H2O2 decreases proliferative activity in ITPA

down-regulated compared to normal HUVEC cells. The

proliferation of treated cells was significantly lower than in

the control wells (p<0.05).

Keywords: Inosine triphosphatase (ITPA), H2O2,

Endothelial cells

https://www.healthline.com/health/allergies

https://www.healthline.com/health/allergies/allergic-reaction

93

Bioinformatically study of D1 and D2 proteins

in two species of Chlorella

Mona Hassanzadeh*, Saleh shahabivand, Ahmad Aghaee

Department of Biology, Faculty of Basic Sciences, Maragheh University, Iran

* Corresponding Author: [email protected]

Green algae are the main group of algae that have the

highest number of species with the highest global

distribution. The genus of Chlorellahas a thin and

spherical with side or cupola chloroplast. In this work, the

nucleotide sequences of Chlorella vulgaris and Chlorella

sorokiniana and the protein sequences were obtained from

NCBI and UniProt database. Then by using the Blast tool,

PSIPRED, RaptorX and MEGA7 software programs, two

different green algal species were examined. By using

these software programs analysis such as similarities

between different sequences, prediction of second and

third structures of the protein anddrawing phylogenetic

tree were carried out. The Blast tool was used to determine

similarities between the obtained sequences of these genes

and the sequences of the same gene in NCBI gene bank as

well as sequences itself. Nucleotide sequences of D1 and

D2 in vulgaris and sorokiniana were obtained and the

percentages of homology between D1-D1 and D2-D2 were

93% and 90% respectively. PROSITE software was used

to evaluate the domain function. These proteins have one

major domain and amino acid length in protein D1 in both

species were 353 and in D2 were 352, molecular weight,

isoelectric point and number of structural amino acids in

D1 and D2 were obtained from isoelectric.ovh.org. The

results showed a remarkable similarity in the nucleotide

sequences, amino acids and number of alpha and beta

structures and third structure of the protein in studied

species. In order to study phylogenetic relationships

between different green algal species, multiple

alignmentswas performed using Clustalw software.

Drawing phylogenetic tree was performed using the

neighbor-joining method of MEGA7 software.

Keywords: Photosystem (II), Green alga, Protein,

Bioinformatical study

Application of bioinformatics in the design of

anti-VEGF peptide

Samaneh Ghasemali1, 2, Safar Farajnia2*, Abolfazl Barzegar3, Mohammad

Rahmati-Yamchi4, Roghayyeh Baghban1, 2 1 Medical Biotechnology Department, Faculty of Advanced Medical

Science, Tabriz University of Medical Sciences, Tabriz, Iran 2 Biotechnology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran 3 Research Institute for Fundamental Sciences (RIFS), University of

Tabriz, Tabriz, Iran 4 Department of Clinical Biochemistry, Faculty of Medicine, Tabriz

University of Medical Sciences, Tabriz, Iran

* Corresponding Author: [email protected]

New blood vessel formation (angiogenesis) is fundamental

to tumor growth, invasion, and metastatic dissemination.

The vascular endothelial growth factor (VEGF) signaling

pathway plays pivotal roles in regulating tumor

angiogenesis.VEGF-A shows prominent activity with

vascular endothelial cells, primarily through its

interactions with the VEGFR-1 and VEGFR-2 receptors

found prominently on the endothelial cell membrane.The

aim of this study was the design of an anti-VEGF-A

peptide based on VEGFR2 binding regions.The effective

amino acid sequences in the interaction of VEGF-A and

VEGFR-2 were investigated by using the valid

bioinformatics software including Chimera and SPDBV

and the binding sites of the VEGF-A molecule on the

receptor were identified. These areas were considered as

the basis for peptide design.In the next step, the sequence

was mutated in the binding regions.The binding ability and

peptide tendency were then analyzed using HadDock, Hex

and ClusPro software. The bioinformatics analysis showed

that the CDR3 region of the receptor played a major role in

binding to VEGF-A And the peptide derived from this

region after a computerized mutation, has the ability to

bind with a greater tendency than VEGFR-2 to target the

molecule. The present study showed that anti-angiogenesis

peptide design is an effective method for inhibiting cancer

growth.

Keywords: VEGF-A, VEGFR-2, Angiogenesis


https://www.pubfacts.com/author/Mohammad+Rahmati-Yamchi

https://www.pubfacts.com/author/Mohammad+Rahmati-Yamchi


https://en.wikipedia.org/wiki/Endothelium

94

Bioinformatics predictionof long-non coding

RNAs as expression regulatory candidates of

genes involving in myelination

Shahrzad Askari*, Fatemeh Khani-Habibabadi, Mehrdad Behmanesh

Department of Genetics, Faculty of Biological Sciences, University of Tarbiat Modares, Tehran, Iran

* Corresponding authorl: [email protected]

Multiple sclerosis (MS) is a chronic neuroinflammatory

disease of the central nervous system, afflicting

approximately 2.5 million people worldwide, and imposes

a great personal and socioeconomic burden. In the most

common form of MS, relapsing-remitting, the

demyelinated lesions will undergo remyelination and result

in remission, after each rout. But in the passing time,

improvement during each remission wanes, and about 80%

of patients go on to develop secondary progressive

multiple sclerosis, which shows accumulative disabilities.

Therefore, understanding dysregulated mechanisms

involved in repair of lesions and remyelination would be

important in order to help to improve the quality of

patient's life. CNTF, NTF3, FGF2, and PDGFC as secreted

factors from astrocytes and affecting myelination, were

chosen. All of the transcription factors (TFs) affecting

these genes were searched. Using the UCSC database, the

valid TFs, and using JASPAR database, the predicted ones

with a high score were chosen. The TFs common among

all of the target genes were chosen. Long non-coding

RNAs (lncRNAs) related to these TFs were obtained using

Lncrna2Target database and literature review. Exerting

some criteria, like the studied role of candidate lncRNAs

in inflammation or other neurodegenerative diseases,

narrowed down the list of candidate lncRNAs as a possible

regulator of transcription of target genes. Obtained data

showed that about 5 lncRNAs have the potential to affect

the expression regulation of target genes. Differential

expression and functional analysis of lncRNAs as the

important factors involved in the regulation of gene

expression would pave the way in understanding the

impaired mechanisms of repair and remyelination.

Keywords: Multiple sclerosis, Myelination, Long non-

coding RNA

Designing, synthesizing and cloning of equine

follicle stimulating hormone in prokaryotic

host

Mohsen Mobini*, Mehdi Rahimpour, Mostafa Shakhsi Niaie

Department of Genetics, Faculty of Basic Sciences, Shahrekord University


Biotechnology is increasingly engaged in various fields of

science and industry to create new products or improve

existing conditions, and industry and animal science are no

exception. One of the most important biotechnology

approaches in this regard is the reproductive screening of

livestock by biological means and processes for the

production and regeneration of livestock with particular

and prominent traits or increasing the proliferation of a

particular type of one. This issue is important not only in

terms of economics but also in aspects such as the survival

of species at risk of destruction and the preservation and

management of alleles and genes in the environment. In

this study, after evaluating and optimizing the horse's

follicular stimulus hormones gene (eFSH), the gene was

synthesized in the puc57 vector that also possesses the

antibiotic resistance gene of ampicillin. The vector was

transferred to the host of the E. coli prokaryotic virus to

propagate and after recombinant colonization was cultured

on the selected medium. The vector was transferred to the

prokaryotic host E. coli virusto propagate and after

culturing on Selective culture medium the recombinant

colonies was extracted. For these colonies, the molecular

parameters of colony PCR were defined and performed,

which confirmed the amplification of the desired gene.

Keywords: Prokaryotic host, Cloning, eFSH

95

Investigating the anti-Alzheimer's properties

of Desf: studying the inhibitory effects of the

Desf extract on the production of amyloid

nanobiofibrils and measuring its antioxidant

activity

Mahdieh Daneshjo, Amir Arasteh*

Department of Biology, Rasht Branch, Islamic Azad University, Rasht, Iran


Desf, known as Heracleum persicum and the English name

Desf, is a flowering plant and perennial and which is from

the Apiaceace family. This plant grows in the mountainous

regions of Iran and despite the various antioxidants among

pimpinellin, It can have significant therapeutic effects in

the treatment of Alzheimer's disease. The purpose of this

study is to investigate the anti-Alzheimer's properties of

Desf by the inhibitory effect of the Desf extract on the

production of amyloid nanobiofibrils and measuring the

antioxidant activity of Desf. Firstly, powdered Desf and

with using 96% ethanol, The hydroalcoholic extract of the

product was prepared. The anti-Alzheimer's effect of Desf

was investigated by investigating the inhibitory effect of

Desf extract on the production of amyloid nanobiofibrils

from bovine serum albumin as a protein model, At

concentrations of 0/4, 0/8, 1/2, 1/6 and 2 mg/ml the extract

was examined by spectrophotometry method and the

percentage of antioxidant activity in doses of 1 to 10

mg/ml was determined by the DPPH method. It was found

that hydroalcoholic extract of Heracleum persicum had the

most inhibitory effect on the production of amyloid strands

at a concentration of 1/6 mg/ml and the highest antioxidant

activity was observed at 3 mg/ml of extract. Inhibition of

the production of amyloid strands and antioxidant effect,

they confirm the anti-Alzheimers properties of the

Heracleum persicum and it can be introduced as one of the

most effective medicines to reduce the effects of

Alzheimer's disease in humans. Keywords: Desf, Anti-Alzheimer, Amyloid

nanobiofibrils, Bovine serum albumin

Evaluation of Limonene synthase gene

expression in Peppermint plants under abiotic

stresses

Shadi Farahmand, Yousef Mohammadi* 1 Department of Biology, Faculty of Basic Sciences, University of Islamic Azad, Tabriz Branch


Peppermint is a very valuable medicinal plant that has

many uses in the pharmaceutical, cosmetic and sanitary

industries due to the presence of menthol. In the

production of menthol, the Limonene synthase gene

converts Geranyl Diphosphate to Limonene. Abiotic

stresses play an important role in the quality and quantity

of menthol. In order to investigate the role of abiotic

stresses in decreasing or increasing the gene expression,

rhizomes of peppermint were cultured in Murashige and

Skoog (MS) medium under drought (0-50-100 150 mM

mannitol), salinity (0-50-100 mM sodium chloride) and

temperature (23, 26 and 29 °C). All experiments were

performed based on a completely randomized design with

two replications. First, the extraction of RNA and cDNA

synthesis was performed for all plants. Real-time PCR

method was used to determine the expression of the

limonene synthase gene and then the expression of the

gene was measured under these stresses. The results

showed that the highest expression of the gene was in the

treatment of zero mannitol, 100 mM sodium chloride and

23°C. In general, it was concluded that the expression of

the limonene synthase gene under the stresses of salinity,

drought, and temperature in the peppermint plant is

affected. Gene expression is decreasing under drought

stress and temperature (with increasing intensity of applied

stresses), while in salt stress by increasing the amount of

NaCl, the expression of the limonene synthase gene is

increased.

Keywords: Abiotic stresses, Gene expression, Limonene

synthasegene, Peppermint

96

Study of the expression of isopiperitenone

reductase gene in peppermint (Mentha

piperita)

Amir Ekhtiyari, Yousef Mohammadi*, Mohammad Reza Mashayekhi

Department of Biology, Faculty of Basic Sciences, University of Islamic Azad, Tabriz Branch


Peppermint (Mentha piperita) has been considered due to

the presence of menthol secondary metabolite.

The isopiperitenone reductase enzyme plays an important

role in the production of menthol, and decreasing or

increasing the expression of the isopiperitenone reductase

gene leads to a decrease or increase in the amount of

menthol. The amount of menthol production in this plant is

heavily influenced by environmental factors. In order to

investigate the effect of salinity, drought and temperature

stress on the amount of isopiperitenone reductase gene

expression in the pathway of menthol biosynthesis, 36

peppermint plants under drought (0, 50, 100 and 150 mM

mannitol), salinity (0 , 50 and 100 mM sodium chloride)

and temperature (23, 26 and 29°C) were cultured. After

two weeks, RNA extraction and cDNA synthesis were

performed and the gene expression level was evaluated

using Real-Time PCR. The results showed that with

increasing mannitol and sodium chloride level, a

significant decrease was observed in gene expression, but

with increasing the temperature from 23° to 29

°C, there

was no significant decrease in the expression

of isopiperitenone reductase gene. Also, the results

showed increasing the temperature from 26°C to 29

°C,

there was a significant decrease in the expression of

isopiperitenone reductase gene. Overall, the results of this

study show that the expression of this gene decreases with

increasing temperature, drought, and salinity.

Keywords: Drought, Isopiperitenone reductase,

Peppermint, Salinity, Temperature

Investigation of changes in the expression of

RNA-helicase (MOV10L1) gene in transgenic

embryonic stem cells of rat exposed to retinoic

acid

Mohammad Shokrzadeh1, Ali asghar Ahmadi2, Azadeh Kazemi3*

1 Pharmaceutical Sciences Research Center AND Department of Toxicology, School of Pharmacy, Mazandaran University of Medical

Sciences, Sari, Iran 2 Fatemeh Zahra Infertility and Reproductive Health Research Center, BabolUniversity of Medical Sciences, Babol, Iran 3 Genetic Department, Sana institute of Higher Education, sari, Iran


The Moloney leukemia virus 10-like 1 (Mov10l1) gene is

specifically expressed in germ cells. Changes in the

expression of this gene in presence of retinoic acid in the

ESCs have been investigated. We conducted an

experimental study to evaluate the Mov10l1 variation in

transgenic OCT4-GFP ESCs (Gift from Max-Planck-

Institute) when incubated at 5 and 10 μM retinoic acid.

Time dependency at 7, 14 & 21th days was also figured

out. SPSS and Excel software were used for data analysis.

By comparing the CT of MOV10L1 gene with the

housekeeping gene (HPRT), results indicated that the gene

expressed 301 times than ESC in all experimental groups.

The expression rate of the gene in the 5 μm group over the

three-week period was higher than the 10 μm group.

Various studies showed that; retinoic acid is a potential

inducer for differentiation of stem cells. It can differentiate

ESC into the neuron, germ like cell and other cells,

depending on the concentration and exposure time.

According to scientific sources, the corresponding protein

of this gene has been observed only in testicular tissue and

mRNA is expressed mainly in testicular tissue and less in

epididymal, endometrial and bile ducts. Therefore, based

on the results, it could be expected the germ cells and

reproductive-related tissues in the experimental conditions.

Keywords: Stem Cells, Mov10l1, Retinoic acid


97

Investigation of MOV10L1 gene expression in

embryonic stem cells of OCT4-GFP in rats

affected by retinoic acid and human follicular

fluid

Mohammad Shokrzadeh1, Ali asghar Ahmadi2, Azadeh Kazemi3* 1 Pharmaceutical Sciences Research Center AND Department of Toxicology, School of Pharmacy, Mazandaran University of Medical

Sciences, Sari, Iran 2 Fatemeh Zahra Infertility and Reproductive Health Research Center, BabolUniversity of Medical Sciences, Babol, Iran 3 Genetic Department, Sana institute of Higher Education, sari, Iran


Nowadays, the researchers have taken promising steps to

treat infertility using stem cell technology by

differentiating stem cells, e.g. embryonic stem cells

(ESCs), into male and female germ cells in vitro. The aim

of this study is to examine the effect of human follicular

fluid (hFF) on the differentiation of mouse ESCs. In this

experimental study, transgenic OCT4-GFP ESC (Gift from

Max-Planck-Institute) cultured with hFF alone (two

concentrations: 25% and 15%) and along with retinoic acid

at three times (7, 14, 21 days). The results showed that in

all periods of time, hFF resulted in decrement of Mov10l1

expression in comparison with control group. The hFF

25% group reduced Mov10l1gene expression more than

the hFF 15% group, in all three time periods studied. In

combined groups, the expression of this gene is higher

than those groups treated merely with hFF. hFF contains

various compounds and hormones, can be effectively play

a role in differentiation. The present study has shown that

mouse ESCs could be differentiated in the presence of

hFF, but there is no evidence to suggest that it should be

male germ cells. However, the presence of retinoic acid is

able to stimulate gene expression.

Keywords: Follicular fluid, Stem Cells, Mov10L1, Gene

Expression, Retinoic acid

Post-training administration of morphine

alters expression of mir33 in rat

Behrang Alani1*, Abolfazl Ardjmand2, Sadegh Moradi2 1 Department of Applied Cell Sciences, Faculty of Medicine, Kashan University of Medical Sciences, Kashan, Iran 2 Physiology Research Center, Kashan University of Medical Sciences,

Kashan, Iran * Corresponding author: [email protected]

The importance of non-coding RNA involved in biological

processes such as learning and memory has become

prominent in recent years. Micro RNAs (miRNAs)

represent a class of small regulatory non-coding RNAs that

mediate gene silencing by identifying specific sequences in

the target messenger RNAs (mRNAs). In addition,

morphine administration at different times relative to

training or testing has different effects on animals learning

and memory. The aim of the current study was to

investigate the expression of mir33 following post-training

administration of morphine in the rat.To determine the

expression of mir33 we used PCR on hippocampal

samples. We used post-training administration of different

doses of morphine (2.5, 5, or 7.5 mg/kg/ip) in an inhibitory

avoidance model of memory. The relative expression of

mir33 in different morphine-treated groups was compared

with the sham and control groups. the comparison of

relative expression of mir33 between sham and control

groups showed no difference (p>0.05). However, the

comparison of relative expression of mir33 between the

morphine-treated groups and the control showed a

significant difference (p<0.05).The post-training

administration of different doses of morphine in the rat

alters the relative expression of hippocampal mir33.

Keywords: Mir33, Learning, Memory, Rat, PCR


98

A study on bioinformatical properties of gene

5a among different strains of infectious

bronchitis virus

Raheleh Majdani*, Zeinab Rusta

Department of Biology, Faculty of Basic Sciences, University of Maragheh, Maragheh, Iran


Infectious Bronchitis (IB) is a highly contagious viral

respiratory disease of poultry characterized by

tracheitis,coughing, and sneezing. It affects the quality and

quantity of meat and egg production in poultry worldwide.

Controlling of the disease is based on regular vaccination

against homologous strains. The identification and

molecular analysis of circulating strains could be very

helpful in poultry farms. IBV (Infectious Bronchitis

Virus), the causative agent of IB and a member of the

coronaviridaefamily, has an RNA genome with the length

of about 27.6 kb. The genome of the virus encodes four

structural proteins (S, E, M, N) and two nonstructural

proteins (3 and 5). Gene 5 has been explained as an

important factor in the pathogenesis of the virus. In this

study, 5a gene nucleotide sequences of different IBV

reference strains were obtained from GeneBank and

molecular analysis of the strains was analyzed using

Bioedit and Mega7 software programs bioinformatically.

Based on sequence similarity analysis, the rate of

relatedness of the strain pairs was between 81%- 100%.

Strains from US, Netherland, and China had 100%

nucleotide similarity and some strains of China showed the

lowest similarity with Taiwan IBV strains. Results of

phylogenetic tree revealed that some Asian strains were

clustered with Australian strains together while some other

Asians were grouped with American strains. Clustering of

IBV strains in different groups regardless of their

geographical regions, can confirm the possibility of

important functional properties for gene 5a.

Keywords: Infectious bronchitis virus, Poultry,

Bioinformatics, Phylogenetic tree, Gene 5a

Bioinformatic analysis of gene 5b from

different strains of infectious bronchitis virus

Raheleh Majdani*, Roya Hatefirad, Mohadeseh Ghaffari, Hamideh

Lamakan

Department of Biology, Faculty of Basic Science, University of

Maragheh, Maragheh, Iran


Infectious bronchitis virus (IBV) is a pathogen causes

acute and highly contagious disease infectious bronchitis

(IB) in domestic chickens. The virus belongs to group III

of genus Coronavirus in the family of Coronaviridae. It

has an unsegmented, positive sense, single-stranded RNA

(ssRNA) genome of approximately 27.6 kb in length and

contains at least 10 open reading frames (ORFs) included:

5 -1a-1b-S (S1, S2)-3a-3b-3c (E)-M-5a-5b-N-Poly (A)-3.

Gene 5, encoding two accessory proteins 5a and 5b, has

been explained as an important factor in the pathogenesis

ofthe virus. In this study, the nucleotide sequences of

OpenReadingFrame (ORF) 5b of gene 5 of different IBV

reference strainswere obtained from GeneBank and

molecular properties of the strains were analyzed

usingbioinformatics software programs, Bioedite

andMEGA7. Based on 5b nucleotide analysis, the rate of

similarity among different strains of IBV was 86%-100%.

Some strains from distinct geographical regions also had

100% similarity. The lowest similarity was observed

between Australian strains with other IBVs (86%-95%).

Based on the results of the phylogenetic tree of gene 5b,

Australian strains grouped together in a distinct cluster

while other regions IBVs were classified differently

regardless to their originated regions. The high rate of

similarity between different strains from distinct

geographicalregions could confirm the important role of

gene 5b in virus vital functions.

Keywords: Infectious bronchitis virus, Gene 5b,

Phylogenetic tree, Bioinformatics, Open reading frame

99

Spermatogenic and phylogenetic

characterizations of isolated fasciola sp. from

natural host (cattle) in north west of iran

Saber Raeghi1*, Jamal Hallajzadeh1, Ali Soleimani1, Mehrdad Rostami3

1 Department of Laboratory Sciences, Maragheh University of Medical Sciences, Maragheh, Iran 2 Department of Public Health, Maragheh University of Medical

Sciences, Maragheh, Iran 3 Student Research Committee, Maragheh University of Medical

Sciences, Maragheh, Iran


Fascioliasis is a parasitic disease that very important in

livestock industry that caused with Fasciola hepatica or

Fasciola gigantica with different hosts. The objective of

this study was to identify these two species F. hepatica

and F. gigantica based on spermatogenesis and using

nuclear and mitochondrial genes (ITS1, ND1 and CO1)

and have been employed to analyze intraspecific

phylogenetic relations of Fasciola sp. Approximately 150

Fasciola specimens were collected from cattle and stained

with haematoxylin-carmine dye and observed under an

optical microscope to examine for the existence of sperm.

The ITS1 marker was used to identify different Fasciola

and phylogenetic analysis based on ND1 and CO1

sequence data were conducted by maximum likelihood

algorithm. Fasciola samples were separated into 2 groups.

Almost all specimens had many sperms in the seminal

vesicle (spermic fluke) and one fluke did not contain any

sperm in the seminal vesicle. The aspermic sample had F.

gigantica RFLP pattern with ITS1 gene. Phylogenetic

analysis based on NDI and COI sequence data were

conducted by maximum likelihood showed a similar

topology of the trees obtained particularly for F. hepatica

and F. gigantica. This study demonstrated that aspermic

Fasciola found in this region of Iran has same genetic

structures through the spermic F. gigantica populations in

accordance to phylogenetic tree.

Keywords: Fasciola, Phylogeny, North West, Iran

Investigation of microbial agent damaging to

historical and cultural monuments

Parastoo Erfanmanesh, Fateme Hajian

Laboratory of Research Institute of Cultural Heritage and Tourism, Iran * Corresponding author: [email protected]

Maintenance and restoration, restoration and technical study of

cultural and historical property is a category that has a special

place in the arts and sciences in different parts of the world, and

this is important for two reasons: first, that human progress in

this century is unique which in turn has created processes of

destruction and collapse of cultural and historical works, but also

multiplied the speed of the past destructive processes, and,

secondly, rapid progress in various sciences, In particular,

biology has led to new materials and methods to serve this

important. The cultural and historical works of libraries,

reservoirs, and stone memorials are continuously influenced by

the environment and are threatened by harmful agents, including

environmental factors. Many biologic agents cause damage to

fungi, bacteria, insects, lichen, and algae. During research

conducted in the library and documentation center of the Cultural

Heritage Institute from 2008 to 1394, fungi are the most abundant

creatures found in places where organic matter is present. Due to

the high volume of documents, sampling was carried out in

different years in a large number so that statistical analysis can be

carried out on the basis of statistical standards of the results. The

chemical reaction that occurs in fungi leaves a clear burning

effect on the appearance of the effects. Sampling was carried out

based on specific characteristics in terms of specimen conditions,

environmental conditions, type of the genus, proximity to

contaminated samples, and so on. Evaluation of samples taken at

five locations was carried out as follows: 1. Air pollution of the

storage tank documents and the storage location of the films; 2.

Contamination of maps and landscapes; 3. The amount of

contamination of documents on the shelves; 4. Archaeological

static documents (in cartons - classified); 5. The films in the tank.

Selected ships' environments were designed to conduct these

studies in specific skids. Which is based on specific percentages

of fungal and antibiotic environments. The conditions of the

culture were cultured in biological incubators at 27 ° C for 3-7

days (at this time interval, regularly controlled). Then,

macroscopic studies were evaluated in terms of fungal colony,

colony count, and colony growth rate. Some of the fungi

observed on the effects of Aspergillus flavus, Cladosporium and

Alternaria have the highest risk of contamination on books and

historical documents. Samples of lichens were also sampled on

the works of the World Heritage Site of Pasargad. By

microscopic loops and staining them, most of the lichens were

Acarospora stapfiana and Acarospora rosulata. There are

different views on how to deal with them from a repair

perspective. The purpose of this research is to identify the

biologic factors of damage to the historical-cultural effects in

different parts of the country in order to provide protection

strategies and deal with them. In addition, the use of

interdisciplinary science in the world to protect cultural works is

one of the characteristics of the disciplines.

Keywords: Biological factors, Fungi, Bacteria, Insects, Lichens,

Historical-Cultural Heritage


100

Assessment of new antibiotics application for

controlling of bacterial canker of stone fruits

in laboratory conditions

Sevil Nematollahi1*, Kosar Sokri1, Reza Khakvar2 1 Department of Plant Protection, Tabriz Branch, Islamic Azad University, Tabriz, Iran 2 Department of Plant Protection, Faculty of Agriculture, University of

Tabriz, Iran * Corresponding author: [email protected]

Bacterial canker caused by Pseudomonas syringae pv.

syringae is one of the most important bacterial diseases of

plants that can causes canker, leaf spot and die-back in

stone fruit trees and considerable yield losses in these

trees. During the study years, 2015-2016 in different areas

of the Khoy (Firooragh- Rahal- Boozghoosh) were

surveyed and some suspected plant samples with canker

symptom were collected.Based on biochemical tests the

cause of bacterial canker of stone fruits primarily in the

area was identified. Molecular identification of selected

isolates, polymerase chain reaction was performed by the

specific primer. PCR with primers B1 and B2 test results

pathovars Pseudomonassyringaepv. syringae (Pss),

fragment 752 bp from the 3 isolates were amplified view.

Then, the effect of 10 new antibiotics, including

Ciprofloxacin, Clindamycin, Co-amoxiclav, Cefalexin,

Azithromycin, Erythromycin, Ceftizoxime, Cefazolin,

Tetracycline on the population of bacterial isolates of Pss

were evaluated in vitro. Based on the results, all selected

antibiotics can decrease the bacterial population but there

are significant differences in their control capability. Based

on the results, Cefalexin and Erythromycin have the

highest and lowest impact on bacterial growth

respectively.

Keywords: Stone fruit trees, Bacterial canker, Antibiotics,

Pseudomonas syringae pv. syringae

The effect of the common pesticides in the

Khoy city on bacterial canker of stone fruits

Sevil Nematollahi1*, Elham Ojaghi1, Reza Khakvar2 1 Department of Plant Protection, Tabriz Branch, Islamic Azad University, Tabriz, Iran 2 Department of Plant Protection, Faculty of Agriculture, University of

Tabriz, Iran * Corresponding author: [email protected]

Pseudomonas syringae pv. syringae is one of the most

important plant pathogenic bacteria that causes canker, leaf

spot and die-back in stone fruit trees and considerable

yield losses in these trees. During this study, from 2015 to

2016 in different areas of the Khoy (Firooragh- Rahal-

Boozghoosh) many stone fruits orchards were surveyed

and some suspected plant samples with canker symptom

were collected.Based on biochemical tests the causal agent

of bacterial canker of stone fruits in this area was primarily

identified. For molecular identification of selected isolates,

polymerase chain reaction (PCR) was performed using a

specific primer. In PCR assay with primers B1 and B2 on

pathovars of Pseudomonassyringaepv. syringae (Pss), a

752bp fragment was amplified from the 3 isolates.

Theeffect of 10 common and mostly used pesticides in

Khoy, including Diazinon, Phosalone, Chlorpyrifos,

Imidacloprid, Captan, Penconazol, Benomyl, Hexythiazox,

Bromopropylate, and Haloxyfop-R-Methyl were evaluated

on the population of bacterial isolates of Pss in vitro.

Among the selected pesticides, Chlorpyrifos increases the

bacterial population and nine other pesticides include

Diazinon, Phosalone, Benomyl, Penconazol, Haloxyfop-R-

Methyl, Bromopropylate, Imidacloprid, Hexythiazox and

Captan were reduced the pathogen population.

Keywords: Stone fruit trees, Bacterial canker, Antibiotics,

Pseudomonas syringae pv. syringae


101

Frequency of TEM beta-lactamase resistance

gene in patients with urinary tract infections

in Bonab County

Reza Masoomi Jahandizi1*, Mir Kamyar Musavi2 1 Department of Cellular and Molecular Biology, Faculty of Science, University of Maragheh, Iran 2 Department of Biology, Faculty of Science, University of Maragheh,

Iran * Corresponding author: [email protected]

TEMbeta lactamase gene is one of the important plasmid

genes in Enterobacteriaceae which is the cause of over

90% of Escherichia coli isolates resistance to beta lactam

antibiotics. The aim of this study was to detect the

prevalence of antibiotic resistance and TEM gene.During 6

months (June to November 2015) 266 clinicalisolates of

E.coli were collected from laboratories in Bonab County.

Phenotypicscreening and confirmation tests for extended

spectrum beta lactamases (ESBLs)were carried out using

disk diffusion (Kirby Bauer) method. All of the

ESBLproducing isolates were tested by PCR using specific

primers. Our results showed that, the maximum resistance

was seen for ampicillin (67.3 %) and the maximum

sensitivity was seen for imipenem (92.5%). In this study

45 % isolates were multidrug resistance, which showed at

least resistance for three antibiotics. Out of 154 isolates, 58

(37.7%) cases were ESBL producers which 65.51% of

isolates contained TEM gene. This study showed that,

TEM gene encodes over 50% of ESBLs in E.coli.

Therefore, we recommend detection of this gene as a

routine bacteriologic procedure in management of the

nosocomial infections caused by enteric bacteria.

Keywords: Escherichia coli, ESBLs, TEMbeta lactamase

gene, Bonab

Effect of Bifidobacterium strains isolated from

baby feces on Acinetobacter biofilm

Masoumeh Moradi Moghadami, Farzaneh Hosseini*

Department microbiology, Faculty of Basic Sciences, University of Islamic Azad University of Tehran North Branch, Iran


Probiotics are beneficial organisms that have their

therapeutic effects by replacing the microbial flora.

Acinetobacter is one of the most important bacteria that

causes the infection of the hospital and has high resistance

to antimicrobial agents. The probiotic Bifidobacterium has

a significant effect on Acinetobacter biofilms and can be

considered as an alternative antibiotic product. In the

laboratory, 100 samples of skin scars and urine twelve

strains of Acinetobacter were confirmed by standard

biochemical tests and for biofilm formation, they were

selected on a polystyrene plate. Biofilm formation was

confirmed through ELISA Reader. The stools of six

infants were cultured in MRS Broth (Man-Rogasa-Sharpe)

medium. After isolation and identification,

Bifidobacterium strains were added to biofilm in different

dilutions. According to the average results of both series of

repeat tests, 75% of Acinetobacter strains were affected by

bifidobacterium and 62.5% of the strains were influenced

by the antibiotic ciprofloxacin. The probiotic

Bifidobacterium has a significant effect on Acinetobacter

biofilms and can be considered as an alternative antibiotic

product.

Keywords: Bifidobacterium, Biofilm, Acinetobacter

102

Study of the antibiotic resistance pattern and

frequency of streptomycin, trimethoprim,

gentamycin, sulfonamides and

chloramphenicol resistance genes in

Escherichia coli isolated from urinary tract

infections of women in Tabriz city

Saman Mahdavi1*, Masoumeh Hasannezhad2, Samaneh Sarrafzadeh3,

Naeemeh Kazemzadeh1

1 Department of Microbiology, Faculty of Basic Science, Islamic Azad

University, Maragheh Branch, Iran 2 Department of Microbiology, Faculty of Basic Science, Islamic Azad

University, Sarab Branch, Iran 3 Department of Biotechnology, Faculty of Basic Science, Islamic Azad

University, Maragheh Branch, Iran * Corresponding author: [email protected]

Urinary tract infection is one of the most common diseases

in human societies. Unfortunately, exorbitance

consumption of antibiotics has caused gradual resistance in

bacteria. The aim of this research was tostudy of the

antibiotic resistance pattern and frequency of

streptomycin, trimethoprim, gentamycin, sulfonamides and

chloramphenicol resistance genes in Escherichia coli

isolated from urinary tract infections of women in Tabriz

city. In this cross-sectional descriptive study, 20 samples

of E. coli isolated from urinary tract infections in women

were tested for surveying of frequency of aadA1, dfrA1,

aac, sul1, cmlA and cat1 genes by PCR method.

Furthermore, antibiogram test was performed for studying

of antibiotic resistance of these isolates by Kirby Bauer

method. The frequency of sul1, cat, dfrA1, aac, cmlA and

aadA1 genes were 10%, 0%, 0%, 0%, 0% and 0%,

respectively. The most antibiotic sensitivity was related to

cotrimoxazole (60%) and nalidixic acid (45%). The most

antibiotic resistance was reported to gentamicin (85%) and

streptomycin (75%), respectively. The increase of

antibiotic resistance can represent the exorbitance

consumption of antibiotics, therefore more appropriate

actions are taken for using of common therapeutic methods

and doing the exact antibiogram test is an inevitable

necessity before prescribing an antibiotic.

Keywords: Escherichia coli, Antibiotic resistance,

Urinary tract infection, PCR

Oral administration of Lactobacillus

rhamnosus has improving effects on burn

wound healing in rats

Amir Abbas Barzegari1*, Masood Hashemzaei1, Reza Alihemmati2,

Mahdi Emdadi1 1 Department of Biology, Faculty of Basic Science, University of

Maragheh, Iran 2 Department Histology and Embryology, Faculty of Medicine, Tabriz University of Medical Science, Iran


Probiotics are microorganisms that when are administered

in enough quantities to their host have beneficial effects

for them. The use of oral administration of probiotics for

the treatment of some skin problems, especially the

wounds, have been shown in previous research. Because

the effects of probiotics depend on the strain that is used,

the purpose of the current study was to evaluate the effects

of Lactobacillus rhamnosus on the wound healing process.

For this, with a heated aluminum bar deep second- degree

burn wounds were induced on the back of 60 male Wistar

rats. The rats randomly assigned to experimental (received

bacteria in distilled water by gavage), negative control

(received no treatment) and vehicle control (received

distilled water by gavage). The period of the experiments

was 14 days. Measurement of wound healing percent and

microscopic evaluation of the wound area in the days 1, 3,

7 and 14 post-burn were conducted. The results showed

that the wound that received the probiotic bacteria had the

higher percent of wound healing compared to the control

alternatives in the days 7 (P<0.05) and 14 (P<0.01) of the

experiments. Moreover, in the wounds that received the

bacteria, inflammatory response was reduced but the rate

of fibroblastic migration, granulation tissue formation,

andreepithelializationwere increased. Overall,

Lactobacillus rhamnosus had positive effects on the deep

second-degree burn wound healing process.

Keywords : Lactobacillus rhamnosus, Rats, Deep second-

degree burn wounds


103

Identification and isolation of the Iranian

native bacteria producing cellulase enzyme

Farzane Kargar1, Mojtaba Mortazavi2*, Mahmood Maleki2, Shahryar

Shakeri2, Masoud Torkzadeh Mahani2

1 Institute of Science and High Technology and Environmental Sciences,

Graduate University of advanced technology, Iran 2 Department of Biotechnology, Institute of Science and High Technology and Environmental Science, Graduate University of

Advanced Technology, Kerman, Iran


The burning of fossil fuels has created a concern for

unstable petroleum sources, as well as, the rising cost of

fuels. These concerns have shifted global efforts to utilize

renewable resources for the production of a 'greener'

energy. Cellulose is the most abundant biomass on Earth.

Cellulase enzymes are the class of enzymes that produced

by the fungi, bacteria, and protozoans that use the cellulose

as a carbon source and generate cellulolytic. This process

is the hydrolysis of cellulose. Economic and geopolitical

factors (high oil prices, environmental concerns, and

supply instability) led policy-makers to focus more on

renewable energy sources. New scientific advances in the

field of biology and basic technology can generate

significant scientific excellence through metabolic

engineering. There is justified that the full potential of

biofuel production from cellulosic biomass will be

obtainable in the next 10 to 15 years. In this study for this

regard, 72 strains of bacteria were collected from different

regions (Khorasan, Hamedan, Ardebil, Kerman) of Iran,

which had a high chance of producing cellulase. These

bacteria were grown ina culture that containing the CMC.

To ensure the synthesis of cellulase, the liquid M9 media

containing CMC were used and the growth of the bacteria

was investigated. After the screening of cellulases bacteria,

the DNA of these bacteria was extracted. The 16S rDNA

gene of cellulases producing strains was amplified using

standard PCR protocol. Primers 8F and 1541R were used

to amplify the 16S ribosomal gene and analyzed by 1%

agarose gel electrophoresis. Finally, the clean-up PCR

product was subjected to cycle sequencing. The results

show that 12 samples were able in the formation of a clear

area in the culture medium indicates the decomposition of

cellulose and production of the cellulase enzymes. In

following, these strains were identified using 16S rDNA.

The results show that some of these screened bacteria

belonged to the Bacillus sp. In the end, the bioinformatics

analysis and phylogenetic tree of these strains will be

conducted.

Keywords: Cellulosic biomass, Geopolitical, CMC,

Economic

Antibiotic resistance pattern and molecular

characteristics of Staphylococcus aureus

isolated from the nasal carriage of health care

workers in two private hospitals in Tabriz,

Iran

Khalil Maleki Chollu1, Yousef Lotfi Hadi Biglu2, Ali Sadighi3, Leili

Hasheminejad1, Kamyyar Khadivi3, Marjan Rahimi3, Fahimeh Feyzie Sani3, Ali Bahadori4*

1 Sarab Faculty of Medical Sciences, Sarab, Iran. 2 Islamic Azad University, Sarab branch, Sarab, Iran. 3 Medical Laboratory Sciences, Sarab Faculty of medical sciences, Sarab,

Iran. 4 Department of Medical Microbiology, Sarab Faculty of medical sciences, Sarab, Iran.


The pathogenic potential and commensal nature of

Staphylococcus aureus allow for easy transmission

especially the nasal cavity is the main colonization site of

Staphylococcus aureus in the human body. The resistance

of Staphylococcus aureus to commonly used antibiotics is

linked to their ability to acquire and disseminate

antimicrobial-resistant determinants in nature. This study

aimed to determine the molecular characteristics and

antibiotic susceptibility pattern of S. aureus isolates

obtained from the nasal carriage of health care workers

(HCWs). Our study was performed between January 2017

and March 2017 at two privatehospitals in Tabriz, Iran.

Nasal samples were collected from the nasal cavity of

HCWs. Standard microbiological methods were used for

identification of S. aureus isolates. Antibiotic

susceptibility pattern was determined by the disc diffusion

method. Determination of virulence genes was performed

by the PCR method. From a total of 150 nasal swab

samples of HCWs, 34 S. aureus strains (22.6%) including

13 (38.2%) MRSA were isolated. In MRSA isolates the

highest sensitivity was for vancomycin and rifampicin,

with 94%. Overall, 23.5% (8/34) and 94.1% (32/34) of S.

aureus isolates were positive for pvl and hla genes,

respectively. This study also shows that nasal isolates of S.

aureus from healthy ruminants might be a potential

reservoir of antimicrobial-resistance. The most common

risk factors for S. aureus carriage in risk groups were

being male, age ≤ 30 years, and nasal cavity cleaning

habits. The results of the present study indicate that S.

aureus nasal carriage with potential virulence ability still

remains a significant healthcare problem, especially in

hospital environments.

Keywords: Staphylococcus aureus, Antibiotic resistance,

MRSA, Nasal carriage


104

Antibiotic resistance pattern of Escherichia

coli isolated from patients with urinary tract

infection in Sarab, Iran

Khalil Maleki Chollu1, Yousef Lotfi Hadi Beyglu1, Ali Sadighi2, Parizad

Azami 2, Leyla Hashemi Nezhad 1, Mohamad Pourhasan1, Hamed Hasanpour1, Ali Bahadori3

1 Department of Nursing, Sarab Faculty of Medical Sciences, Sarab, Iran 2 Department of Medical Laboratory Sciences, Sarab Faculty of Medical Sciences, Sarab, Iran 3 Department of Medical Microbiology, Sarab Faculty of Medical

Sciences, Sarab, Iran * Corresponding author: [email protected]

Urinary tract infection (UTI) is one of the most prevalent

infectious diseases and Escherichia coli is its common cause.

Therapy for these infections is usually begun before results of

microbiological tests are known. The rationale for this

approach is based on the highly predictable spectrum of

etiologic agents causing UTI and their antimicrobial

resistance patterns. Escherichia coli remains the predominant

uropathogenic (80%) isolated in acute community-acquired

uncomplicated infections. Recent advances in molecular

biology may facilitate the identification of new etiologic

agents for UTI. The need for accurate and updated population

surveillance data is apparent, particularly in light of concerns

regarding antimicrobial resistance. This information will

directly affect the selection of empiric therapy for UTI.The

aim of this study was to assess the resistance patterns of E.

coli in urinary tract infections and to determine the

susceptibility of E. coli to commonly used antimicrobials and

also to evaluate the options for empirical treatment of UTI.

Our study was carried out in the Imam Khomeini Teaching

Hospital of Sarab Medical Sciences Faculty. Sample

collection was done between March 2017 and November

2017; antimicrobial susceptibility tests were done by disk

diffusion method. The diagnosis of UTI WAS based on a

quantitative urine culture yielding greater than 100,000

colony-forming units per milliliter (105 CFU/ml) of urine.

Results were analyzed after using ten commonly used

antibiotics for susceptibility test according to CLSI protocol.

E. coli grew in 115 (115/141) urine samples. Imipenem,

ofloxacin, ciprofloxacin were the most effective antibiotics

(89.3%, 88.1%, and 84.8% respectively). Maximum

resistance was detected for cotrimoxazole, cefixime,

cefotaxime,and ceftriaxone. Knowledge of local susceptibility

patterns is important for the selection of appropriate empirical

therapy for UTI. In conclusion, data from local laboratories

exaggerate the fluoroquinolone resistance problems among E.

coli urine isolates from general practice. Imipenem,

ofloxacin,and ciprofloxacin should be used in empirical

therapy of UTI. For optimal interpretation of cumulative

susceptibility data in the primary healthcare setting, it is

necessary to take into account the type of UTI (uncomplicated

vs. complicated), as well as the sex and age of each patient.

Keywords: Urinary tract infection, Escherichia coli,

Antibiotic susceptibility, Disk diffusion

Evaluation of the frequency of class I integron

gene and antibiotic resistance pattern in

Escherichia coli isolated from patients with

urinary tract infections in Imam Khomeini

Hospital in Sarab, Iran

Ali Bahadori1*, Khalil Maleki Chulu2, Yousef Lotfi Hadi Beyglu2, Ali

Sadighi3, Kamiar Khadivi3, Leyla Hashemi Nezhad2

1 Department of Microbiology, Sarab faculty of Medical Sciences, Sarab-

Iran 2 Department of Nursing, Sarab faculty of Medical Sciences, Sarab-Iran 3 Department of Medical Laboratory Sciences, Sarab Faculty of Medical

Sciences, Sarab, Iran


Urinary tract infections are the second most common cause of

infection in the body of human. Among the causes of urinary

tract infection, E. coli is the most common causative agent of

urinary tract infection (UTI), and UTI-induced antibiotic

resistant E. coli isolates are increasing. Treatment of urinary

tract infections due to antibiotic resistance in agents is getting

difficult every day. Integrons are genetically mobile agents

that promote the spread of antibiotic resistance genes among

bacteria. The purpose of this study is to determine the

antibiotic resistance pattern, to determine the prevalence of

class I integrons and to determine its association with

resistance to antibiotics in isolated Escherichia coli isolated

from urinary tract infections in Imam Khomeini Hospitalin

Sarab. Among 105 positive urine culture samples of patients

with urinary tract infection from patients referred to Imam

Khomeini Hospital in Sarab, 81 samples of Escherichia coli

were isolated and determined by routine biochemical and

microbiological tests. Antimicrobial resistance pattern of the

samples was determined by the diffusion method in

comparison with the antibiotics Ceftazidime,

Imipenem,Trimethoprim, Ampicillin, Gentamicin,

Ceftriaxone, Nalidixic Acid, and Chloramphenicol. After

extraction of bacterial DNA by boiling and freezing method,

the frequency of Integron Class I gene in E. coli isolated from

urinary tract infection was determined using PCR and specific

primers. In isolated samples of patients, the highest antibiotic

resistance was seen to trimethoprim (85%) and nalidixic acid

(74%) and the least resistance to gentamicin (11%). All of

isolated Escherichia coli was sensitive to Imipenem and

showed sensitivity to gentamicin (89%) and chloramphenicol

(85%). The frequency of class I integrin gene was observed in

67 isolated Escherichia coli samples (82.7%). According to

the findings of this study,it has the necessity to use the best

treatment against urinary tract infections and considering the

results of this study, it is advisable to use fewer antibiotics of

Trimethoprim and Nalidixic acid in the primary treatment of

urinary tract infections. The identification of the genes

involved in the development of these resistances is one of the

main needs of the study in the treatment of infections and

class I integrons rate in Escherichia coli in the study is high,

which may have a role in facilitating the spread of multiple

drug resistance Strains.

Keywords: Antibiotic resistance pattern, Escherichia coli,

Class I integron, Urinary tract infection

105

The efficiency of the cold argon-oxygen

plasma for controlling the fungal of

documents in cultural heritage

Nazanin Abedinzadeh1, Hossain Riahi1*, Somayeh Keypour2 1 Faculty of Life sciences and Biotechnology, Shahid Beheshti University, Evin, Tehran, Iran

2 Department of Biology, Farhangian University, Tehran, Iran.


Different artworks, pharmacopeia as well as the history of

different ancient tribes can be found in the paper

manuscript left by them. Fungi are among the most

degradative micro-organisms which deteriorate paper-

based items of cultural heritage. Appropriate conservation

treatments to deal with fungal infections include

mechanical, chemical and biological methods, which can

cause some undesirable effects on the paper itself and

health hazards for humans. Using different radiations are

among those methods which are used for controlling

cellulolytic fungi. Plasma in the physical sciences refers to

an intermediate or almost ionized medium. When the gas

is exposed to an electrical drain, the plasma is produced.

The degree of ionization can vary from 100% to very low

values. Therefore, it seems that the plasma is suitable for

sterilization. The aim of this study is to control Alternaria

sp. and Cladosporium species causing biodeterioration on

some books in the national library of Iran. For testing the

antifungal effect, the cold Argon-oxygen plasma jet was

used against the 250 µl of spores poured into a petri dish.

The treatment was carried out using 1.2 Watt of plasma

and four replications were made for each treatment. The

result showed that the radiation energy of the cold Argon-

oxygen plasma jet had the full control efficiency on

Alternaria sp. but it couldn’t prevent and control

Cladosporium sp. growth.

Keywords: Cultural Heritage, Controlling, Alternaria sp.,

Cladosporium sp., Cold argon-oxygen plasma

Molecular diagnosis of class 1 integrons in

Acinetobacter baumannii strains isolated from

patients admitted in hospitals of Sari

Mina Owrang*, Alam Ara Gholami, Mir Naghi Fazeli Ghadi

Department Microbiology, Faculty of Basic SciencesUniversity of Islamic Azad University Sari branch, Iran


Acinetobacter bumannii is opportunistic, gram-negative,

aerobic, and non-fasting. The increasing resistance to the

latest anti-dementia and high environmental resistance has

made it difficult to control and eradicate this bacterium.

The resistance pattern of this bacterium varies from region

to region. Due to the increased mortality in patients,

determining this pattern for It is necessary to determine the

strategy of coping with this bacterium and the optimal use

of antidepressants. Therefore, the aim of this study is to

investigate the Class II Intronic molecular detection in A.

berumani strains.This study was performed on 28 isolates

of A. bumanni isolated from patients hospitalized in burns

and infectious wards in Imam, Zare and Shafa Hospitals of

Sari in the 6-month period of Bahman 95 to Tir 96. The

antidepressant susceptibility of isolates was determined by

disc diffusion agar method. The presence of Class 1

integrin was investigated by PCR method.In this study, the

highest resistance to amikacin (95%) and the highest

susceptibility to cefotaxime (85%) was observed. Class, I

incontinence was 75% among strains isolated.In this study,

high resistance to different classes of antidepressants in

isolates and the high prevalence of class 1 integron was

observed. Statistical analysis also revealed the relationship

between Class 1 integron and drug resistance in these

strains.

Keywords: Acinetobacter baumannii, Antibiotic

resistance, Integrons

106

A proteomics approach to identify

metacyclogenesis regulated proteins in

Iranian clinical isolates of Leishmania tropica

Nasrin Amiri Dashatan1, Mehdi Koushki2, Nayebali Ahmadi1

1 Proteomics Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran 2 Departments of Biochemistry, Faculty of Medicine, Tehran University

of Medical Sciences, Tehran, Iran * Corresponding author: [email protected]

Leishmania as a protozoan parasite is the etiological agent

of leishmaniasis, which is responsible for a spectrum of

disease. Leishmania parasites including a dimorphic life-

cycle are transmitted by the sand-fly vector. Promastigote

forms in the alimentary tract of sand-fly are extracellularly

flagellated. Metacyclogenesis defines as differentiation

from procyclic to metacyclic promastigotes that correlates

with high Leishmania infectivity. We compared the

proteomes of procyclic and metacyclic promastigotes of L.

tropica in Iranian isolates. Proteins of cell lysates were

extracted and the protein concentration of stages

determined by using BCA assay. After digestion of

proteins with trypsin/LysC, Proteome profiling was done

by LC-MS/MS. in the present study more than 100

proteins were identified in each understudy stages. Among

these, 40S ribosomal protein SA, Proteasome

endopeptidase complex, Putative serine peptidase and

Mitochondrial RNA binding protein 1 were procyclic

specific proteins. Tubulin beta chain and dynein light

chain were detected in the metacyclic stage. This study has

shown that a number of proteins differentially expressed in

procyclic and metacyclic stages of L. tropica isolates.

According to results, proteins associated with protein

synthesis pathway were expressed in procyclic. Motion

proteins were identified in a metacyclicstage in accordance

with infectivity power of metacyclic form.

Keywords: Leishmania tropica, Proteomics, LC-MS

Determination of antibiotic resistance pattern

in Aeromonas hydrophila isolated from

Reared Oncorhynchus mykiss in Marand, Iran

Abolfazl Jafari-Sales1*, Farnaz Rasi-Bonab2, Haedeh Mobaiyen3,

Behboud Jafari4

1 Department of Microbiology, Kazeroon branch, Islamic Azad

University, Kazeroon, Iran. 2 Department of Microbiology, Marand Branch, Islamic Azad University, Marand, Iran. 3 Department of Microbiology, Tabriz Branch, Islamic Azad University,

Tabriz, Iran 4 Department of Microbiology, Ahar Branch, Islamic Azad University,

Ahar, Iran.

* Correspondence author: [email protected]

Aeromonas hydrophila is a gram-negative, positive

oxidase, anaerobic, and opportunistic bacteria that, under

certain conditions, become a pathogen (in humans and

fish). This bacterium causes toxin and host infection in

which different antibiotic resistance in isolated strains has

been reported in different regions of the world. The aim of

this study was to determine the prevalence of this

bacterium and its susceptibility to common antibiotics in

Marand city. 30 fish from 3 Reared Oncorhynchus mykiss

in Marand (For each farm, 10 numbers) were randomly

assigned to suspected fish to the disease. By using

biochemical tests, 9 samples were identified as Aeromonas

hydrophila. Antibiogram for these specimens showed that

the bacterium had the highest resistance to vancomycin

(88.9%) and clindamycin (77.8%) antibiotics. Considering

the different antibiotic resistance pattern in this study and

other similar studies, the necessity of examining the

pattern of resistance in each region seems necessary.

Keywords: Aeromonas hydrophila, Oncorhynchus mykiss,

Antibiotic resistance


107

Evaluation of the effects of silver nanoparticles

and alcoholic extract of Achillea wilhelmsii on

pathogenic bacteria Staphylococcus aureus,

Bacillus cereus, Escherichia coli and

Pseudomonasaeruginosa

Farnaz Rasi-Bonab*, Abolfazl Jafari-Sales, Masoud Taheri

Young Researchers and Elite Club, Marand Branch, Islamic Azad University, Marand, Iran.


In recent years, the use of medicinal plants has increased

in the treatment of infections rather than antimicrobial

drugs. Nanotechnology also plays a major role in the

medical arena, which uses nanoparticles like silver to fight

microbial resistance. The aim of this study was to compare

the effects of silver nanoparticles and alcoholic extract of

Achillea wilhelmsii on some pathogenic bacteria and also

silver nanoparticles.The plant was examined from the

natural areas of Marand city in East Azarbaijan province

and was identified as Achillea wilhelmsii according to

herbarium characteristics in Islamic Azad University of

Marand district. The alcoholic extract of this plant was

prepared and the effect of concentrations of 50 mg / ml,

100 mg/ml, 200 mg/ml, 400 mg / ml of this extract and

concentrations of 10 mg / ml, 20 mg / ml, 40 mg / ml, 80

mg / ml of silver nanoparticles by Well Diffusion Method

on S. aureus, B. cereus, E. coli and P. aeruginosa.

Minimum Inhibitory Concentration and Minimum

Bactericidal Concentration (MIC/MBC) was determined

on bacteria by a dilution method.The results showed that

the inhibition effect of Achillea wilhelmsii on gram-

positive bacteria is more than gram-negative bacteria while

the inhibitory effect of silver nanoparticles on gram-

negative bacteria is more than gram-positive bacteria. The

effect of the combination of Achillea wilhelmsii and silver

nanoparticles was much greater than the effect of each of

them. The composition showed the highest effect on P.

aeruginosa and the lowest sensitivity to S. aureus. The

results of this study showed that the alcoholic extract of

Achillea wilhelmsii is considered to be an antibacterial

properties Medicinal Plants group that can be considered

as an adjunct to further studies for use in vivo conditions

and replacement with common chemical agents in the

treatment of infections.

Keywords: Medicinal plants, Silver nanoparticles,

Achillea wilhelmsii

Assessment of the antimicrobial effect of

Cistanche sp. on the planktonic forms and

biofilm structures of pathogen bacteria

Mouj Khaleghi*, Mehdi Abbasnejad, Farideh Mohammad Hossein Zadeh

Department of Biology, Faculty of Science, University of Shahid Bahonar University of Kerman, Kerman, Iran.

* Corresponding author: [email protected].

Nowadays, the resistance of microorganisms to various

antibiotics is a serious concern. Also, study on herbal

plants is very important. In this study the antimicrobial

effect of the juice of Cistanche sp was investigated on

planktonic and biofilm forms against some pathogens.

Antimicrobial effect of Cistanche sp was determined by

two methods, well and disk diffusion. Seven strains of

standard bacteria, 4 strains of clinical bacteria and 3 strains

of fungi were used. Minimum inhibitory concentration and

Minimum lethal concentration (0.2-50 mg/ml) were

determined by broth macrodilution. Antibiofilm effects,

biofilm formation and biofilm degradation, were studied

by microtiter plate in 5, 10, 15 and 20 mg/ml. According to

the results, the disk diffusion method was better than well

method. The average of inhibition zone in 36.4% of

bacteria was ≥ 10 mm, whereas it was ≤ 9mm in 9.09 of

bacteria. The juices of Cistanche sp in 5 mg/ml could stop

biofilm formation in 63.64% of bacteria. The most stop

biofilm formation was in Escherichia coli PTCC1330 and

Micrococcus luteus PTCC1110 (>80%). The greatest

eradication of biofilm structures was observed in

Micrococcus luteus PTCC1110 (58.75%) and

Pseudomonas aeruginosa (58%). In this study was

investigated that the juice of Cistanch sp has not only

antibacterial but also anti-biofilm effects.

Keywords: Cistanche sp, Herbal Plants, Antimicrobial,

Pathogen Bacteria, Biofilm

http://jhd.iaushk.ac.ir/?_action=article&au=36653&_au=Masoud++Taheri

108

Antimicrobial effect of 5 Nepeta native species

of Kerman Province

Atfeh Iranmanesh, Moj Khaleghi*, Mahdi Hasan Shahian, Firozeh

Bordbar

Faculty of Science, Department of Biology, Shahid Bahonar University

of Kerman, Iran


In recent years, due to the side effects and drug resistance

created by the use of antibiotics, scientists' attention to the

use of medicinal herbs has been reduced with

complications, toxicity and resistance. In this study, the

antimicrobial effects of 5 Nepeta spp. Strains have been

investigated against a number of pathogenic microbes. The

studied microbs included Escherichia coli, Enterococcus

faecalis, Pseudomonas aeruginosa, Staphylococcus

aureus, Bacillus cereus, Klebsiella menomonie, Bacillus

subtilis, Streptococcus mutans, Acinetobacter,

Micrococcus, Staphylococcus epidermidis, Staphylococcus

saprophyticus and Candida albicans. From N. assargens

(extract 1), N. mahanensis (extract 2), N. rivolaris (extract

3) and two N. fissa (N. fissa) subpopulations from the

Dehbakri Mountains (Extract 4) and N. fissa (b) from

Sardoeian Mountains (Extract 5), methanolic extract was

prepared by maceration method. Then, the inhibition zone

of microbs was investigated in a whole method. The

results showed that only bacterial isolates of Bacillus

cereus, Staphylococcus saprophyticus, Klebsiella

menomonie, and Staphylococcus epidermidis had the

inhibition zone, the most effective in these bacteria were

extract 4(17±7 ) Extract 4 (17.3 ± 2.5), Extract 2 (20 ± 8.6

mm) and Extract 1 (25.33 ± 5.33 mm). On the other hand,

it has been shown that these extracts have bacteriostatic

and bactericidal effects.

Keywords: Medicinal plants, native to Kerman,

pathogenic bacter

Studying antimicrobial effect of fermented

probiotic milk using Lactobacillus and

Bifidobacteria strains

Nafisseh Farazandehnia1, Mohamad Reza Fazeli2, Hosein Jamalifar2* 1 IC Pharmacy School, Tehran University of medial science, Iran 2 Pharmaceutics Quality Assurance Research Center and Department of

Drug and Food Control, Faculty of Pharmacy, Tehran University of

Medical Sciences, Tehran, Iran * Corresponding author: [email protected]

Production of the probiotic product could be effective in

health and prevention of diseases especially

gastrointestinal and infections. The aim of this study was

to prepare probiotic fermented milk using various strains

of Lactobacillus casei, Lactobacillus plantarum and

Bifidobacterium bifidum, Bifidobacterium angulatum. In

this study the stability of probiotic milk in three

temperatures condition (4, 25, 37ºC), change of pH, and

antimicrobial effect of probiotic product with using two

standard bacteria Staphylococcus aureus and Salmonella

typhimurium was studied. Maximum growth of probiotic

bacteria (107 Cfu/m) in 18 hours (t=18) after the first time

(t=0), any change in pH of probiotic fermented milk was

not seen and the number of pathogenic bacteria in

probiotic product approximately 3 log reduce that

represents the antimicrobial effect of the probiotic product .

Keywords: Probiotic, Lactobacillus, Bifidobacter,

Antimicrobial effect, Staphylococcus aureus, Salmonella

typhimurium

109

Effect of salt on saliva antibacterial property

on Staphylococcus mutants and

Staphylococcus aureus bacteria

Nasrin Fattahi*, Maryam Mirbagheri

University of Ashraphi, Esfahan, Iran * Corresponding author: [email protected]

Saliva is one of the first defense dams against

microorganisms which enter the body through mouth and

cause diseases, moreover, it decreases affliction to

bacterial diseases by decreasing the microbial population

of bacteria. This research aims to assess the effect of

different salt concentrations and over-consumption of salt

on antibacterial property of saliva on bacteria such as

Staphylococcus aureus, which cause tooth decays, and

Staphylococcus mutase, which cause food poisoning.In

this research, by sampling healthy people’s saliva and

mixing it with different concentrations of sea salt and

iodine-containing table salt using a hole and cultivation

meadow, Staphylococcus aureus and Staphylococcus

mutase bacteria were separately studied. The halo created

in the pure saliva hole in both bacteria was decreased from

1cm to 1mm in the holes containing saliva mixed with

salt.the effect of salt in different concentrations using

micro-dilution was also investigated. Consuming table salt

with 4%, 10%, and 30%, and its mixture with the saliva

and persistence of saltiness in the mouth can decrease the

antibacterial effect of saliva on Staphylococcus aureus and

Staphylococcus mutase, thus, increasing affliction to decay

in most teeth, and food poisoning, and consumption of too

much salt can decease the performance of the first defense

dam in the body against microorganisms.

Keywords: Salt, Saliva, Staphylococcus mutants,

Staphylococcus aureus

Aqueous and ethanolic extracts of Allium

hirtifolium and Allium sativumon growth

inhibition of Candida tropicalis in a systemic

candidiasis mouse model

Alireza Diba, Fahimeh Alizadeh*

Department of Microbiology, Yasooj Branch, Islamic Azad University, Yasooj, Iran


Usually, antifungals such as fluconazole are used for the

treatment of candidiasis, but one of the major clinical

problems is the resistance of Candida species to antifungal

agents. The search for antifungal agents derived from

plants could help in solving this problem. This study

evaluated theeffectsof Allium hirtifolium and Allium

sativum extracts on Candida tropicalisboth in vitro and in

a systemic candidiasis mouse model.In this cross-sectional

study, ten clinical isolates of C. tropicalis were isolated

and identified from immunocompromised patients.

Antifungal susceptibilities and time-kill study of aqueous

and ethanolic extracts of A. hirtifolium and A. sativum

were carried out against C. tropicalis. The efficacy of A.

hirtifolium and A. sativum extracts compared with

fluconazole in alleviating systemic C. tropicalis infections

was evaluated in vivo through a systemic candidiasis

mouse model. The antifungal susceptibilities and time-kill

study results revealed that the A. hirtifolium and A. sativum

extracts were able to inhibit C. tropicalis growth(p < 0.05).

Our results showed that treatments of BALB/c mice with

A. hirtifolium and A. sativum (at 1 mg/kg/day) were

slightly less efficacious than that of fluconazole in terms of

the fungal burden reduction and host survival time, it was

still effective against C. tropicalis. These results

demonstrate the efficacy of anticandidal effects of A.

hirtifolium and A. sativum extracts both in vitro and in an

animal model of candidiasis and affirm the potential of A.

hirtifolium and A. sativum extracts as an adjuvant therapy

in the management of Candida infections.

Keywords: Allium hirtifolium, Allium sativum, Candida

tropicalis, Antifungals


110

Determination of antibiotic resistance pattern

and molecular diagnosis of β -lactamase genes

(blaSHV, blaTEM, blaCTX-M) in Klebsiella

pneumonia isolates from in-patient of Ilam

hospitals

AzarAbassi1*, Mohammadreza Nahaei1, Ahad Mokhtarzadeh1, Arman

Rostamzad2 1 Department of Biology, Higher education Institute of Rab-Rashid,

Faculty of Science. 2 Department of Biology, Faculty of Sciences, Ilam University, Ilam,Iran * Corresponding author: [email protected]

Extended-spectrum β-lactamases producing bacteria have

spread widelythroughout the world. Production of these

enzymes induces resistance to a wide range ofantibiotics in

bacteria, which leads to a limitation of infection control

and correct treatment. The aim of thepresent study was to

investigate the antibiotic susceptibility of Klebsiella

pneumonia isolates to beta-lactams antibiotics and

presenceof β-lactamases (SHV, TEM, CTX-M) genes, in

Klebsiella pneumoniae isolated from clinical samples in

the Ilam city hospitals. In this study, during a one-year

period, 100 Klebsiella pneumoniae isolates obtained from

patients hospitalized in the Ilam city. All isolates were

identified by standard biochemical tests and susceptibility

to 11 antibiotics that traditionally use in the treatment of

bacterial infection was evaluated using the disk diffusion

method. The ESBL producing isolates were identified

using the combined disc Test and extended-spectrum beta-

lactamase (TEM, SHV, CTX-M) genes were detected

using PCR method. The highest rate of antibiotic

resistance among Klebsiella pneumonia isolates was

related to ciprofloxacin (100%) and the minimum rate of

resistance was related to Imipenem (20 ) (pv≤0.05). Out

of 91 isolates were identified as ESBL producing using

combined disc method. The PCR results showed that 79

isolates (79%) consisted of CTX-m gene, 91 isolates

(91%) were consisting of SHV gene and 77 isolates were

containing TEM gene. The high rate of resistance to ESBL

enzymes in this study is a greatconcern, so to infection

controlling and preventing of spreading drug resistance

bacteria highlights the need for infection control measures

including antibacterial management andidentification of

resistant isolates.

Keywords: Klebsiella pneumoniae, Ilam, Beta-lactamase,

blaSHV, blaTEM, blaCTX-M

The evaluation of beta-lactamases genes in

E.coli species isolated from hospitals sewages

in Ilam city

Arman Rostamzad1*, Mina Taheri2 1 Department of Biology, Faculty of Science, University of Ilam, Iran 2 Department of Biology, Faculty of Sciences, Ilam University, Iran


The wide application of antimicrobial agents in hospitals

has led to large-scale dissemination of antibiotic-resistant

bacteria in different environments. The aims of present

study were to evaluation of antibiotic resistance in E.coli

species isolated from Ilam hospitals wastewater and

detection of extended-spectrumbeta-lactamasesblaTEM,

blaSHV, blaCTX-M and FOX genes. Out of 81 E.coli

species during one year period (from September 2014 to

September 2015) isolated from wastewater samples given

from hospitals in Ilam city. Samples were identified using

routine microbiological and biochemical tests. The

antibiotic resistance pattern of isolates to 14 different

antibiotics has done by disk diffusion method and ESBL

producing strains were characterized by phenotypic

method as CLSI guideline and combination disk,

phenotypic detection of AMPC producing isolates was

performed using sensitivity to cefoxitin (30µg) and

frequency of blaTEM, blaSHV, blaCTX-M-15 and blaFOX

genes were evaluated by polymerase chain reaction (PCR).

The antibiogram results showed that, the highest rates of

resistance were related to amoxicillin (100%) and

ampicillin-sulbactam (91.36%), and the highest rate of

sensitivity were related to meropenem and imipenem

(100%), among 81 species totally 30 isolates (37.3%) were

detected as ESBL producing by phenotypic and double

disk tests, and results of PCR showed that frequency of

blaTEM, blaSHV, blaCTX-M-15 and FOX genes were

43.33%, 6.66%,20% and 4.93% respectively. The results

of this survey showed that, thebacterial strains isolated

from the hospital wastewater exhibited about 44% of the 4

investigated resistance genes, which could be further

disseminated among environmental bacteria.

Keywords: Antibiotic resistance, beta-lactamases genes,

E.coli, Hospital sewage


111

Screening of L-asparaginase producing

strains isolated from honey in different

regions of Iran

Ziba Babazadeh fardi1, Babak Elyasifar2, Azita Dilmaghani* 1 Department of Biology, Faculty of Sciences, University of Maragheh, Iran 2 Department of Pharmaceutical Biotechnology, Faculty of Pharmacy,

Tabriz University of Medical Sciences, Tabriz, Iran * Corresponding author: [email protected]

L-asparaginase is a well-known enzyme that is used as one

of the most effective anti-leukemic drugs. Also, another

important application is in the food industry.L-

asparaginase is the first anti-tumor activity enzyme that

has been widely studied in humans. In this study, the

production of L-asparaginase by 30 microorganisms

isolated from honey from different regions of Iran was

screened. Modified M-9 agar medium was used for

qualitative analysis. Three strains produced L-

asparaginase. Most of L-asparaginase producers belonged

to Bacillus sp. based on 16S rRNA analysis. Since L-

asparaginase isolated from different bacteria has different

anticancer effects, searching for microorganisms that

produce this enzyme is one of the main ways to achieve an

enzyme with ideal therapeutic properties.

Keywords: Cancer, 16S rDNA, Bacillus, L-asparaginase

Isolation of fast growth and acid resistance

probiotics from Golpayegan yogurt

Nadia Ramezani, Mozhdeh Torkzaban, Mohammad Pooya

Naghshbandi1* Department of Microbial Biotechnology, School of Biology, College of

Science, University of Tehran, Tehran, Iran


Probiotic microorganisms are nutritional supplements that

play an important role in human health. Daily use of

industrial dairies has led to an increase in the elimination

of these probiotics. The purpose of this experiment is to

isolate acid-resistant probiotic microorganisms with a

rapid growth rate from local yogurts in Golpayegan and

use them in industrial production. Material and Method: In

this experiment, which was carried out in 2018,

Microorganisms were isolated from a sample of local

yogurts in Golpayegan province using MRS. Resistance

effects of these microorganisms on gastric and uric acid

were investigated through culture in acidic media, also

their effect on pathogenic microorganisms was analyzed

using disk diode method. Results: At this stage, acid-

resistant yeasts were found in a specific culture medium

(MRS). After culture in acidic media, the resistance to pH

2.5 was confirmed. The diameter of the growth halo of

pathogenic bacteria was also measured. Result: The

isolated yeast has a much higher growth rate than other

microorganisms within the medium and has the ability to

colonize in the acidic environment of the stomach and

intestines. It also has the ability to prevent the growth of

pathogenic bacteria.

Keywords: Probiotic, Acid resistance, Yoghurts,

Golpayegan

http://bjm.ui.ac.ir/?_action=article&kw=89032&_kw=L-asparaginase

112

Designing a novel signal peptide for secretion

of recombinant Human activin A protein

through the twin-arginine translocation (Tat)

pathway in E. coli

Zahra Hajihassan, Farshid Zandsalimi* 1 Faculty of New Sciences and Technologies, University of Tehran, Tehran, Iran


One of the most prominent features of the E. coli, which

makes it widely used in the production of recombinant

proteins, is the ability of secretion of the desired protein

into the extracellular space. The twin-arginine pathway

(Tat), due to the possibility of secretion of the recombinant

protein in the folded state, has a high potential for

facilitating downstream processes of restoring the 3D-

structure and hence the function of proteins. In this project,

regarding the key role of signal sequences in bacterial

protein secretion, we gathered the signal sequences from

two Archaea Haloferax volcanii and Halobacterium

salinarumand E. coli itself from the UniProt database and

then the eight sequences were aligned by Clustal Omega

software. The final sequence with conserved and common

amino acids was obtained. To confirm the secretion of

human activin A linked to the designed signal peptide,

further studies were carried out with TatP, PRED-Tat, and

Phobius software, all of which are signal peptide indicative

tools. The output scores, in addition to confirming the

ability of the designed signal peptide in Human Active A

secretion, indicating its specificity for the Tat pathway.

The key step in translocation of protein to the extracellular

space is the identification of the signal sequence by its

receptor on the inner surface of the membrane.

Comparison of the interaction energy of the designed

signal peptide and its receptor with the homologous in E.

coli with the foldX software showed more efficient

bacterial secretion.

Keywords: The twin-arginine translocation (Tat), Human

Activin A, Signal peptide

Investigation of antimicrobial properties of n-

hexane extract of Onosmastraussii

Amirarsalan Kavyanifard1*, Nasrollah Soori2

1 Department of Biology, Faculty of Basic Sciences, Payame Noor University. 2 Department of Horticulture, Faculty of Agriculture, Payame Noor

University. * Corresponding author: [email protected]

Onosma is an important genus of Boraginaceae family.

Lipophilic extracts of roots of various species of this genus

are used for wound and burns healing in west regions of

Iran, of them Lorestan, for decades. The positive effect of

this extracts is for their antimicrobial properties. In this

study, using disk diffusion method, the effect of n-hexane

extract of Onosmastraussii root on Acinetobacter

baumannii, Bacillussubtilis, Staphylococcusaureus and

Pseudomonasaeruginosa bacteria and Aspergillusflavus,

Candidaalbicans and Fusariumsolani was investigated.

Our results showed that the extract inhibits S. aureus, A.

baumannii, P. aeruginosa, A. flavus and C. albicans

growth, but not about B. subtilis and F. solani. This is the

first investigation about antimicrobial effects of n-hexane

extract of O. straussii.

Keywords: Staphylococcusaureus, Acinetobacter

baumannii, Pseudomonasaeruginosa, n-hexane extract,

Onosma

113

Effect of synergist Aloe Vera extract and

supernatant Lactobacillus fermentum from a

local cheese (Kozeh) on Klebsiella bacteria

Saeede Heydarzadeh1, Amir Tukmechi2* 1 Department of Biology, Faculty of Basic Scince, Azad University of Urmia, Iran 2 Department of Microbiology, Faculty of Animal medicine, University of

Urmia, Iran * Corresponding author: [email protected] @gmail.com

Common problems in the medical world have been

including bacteria’s resistance against antibiotics.

Considerably, Aloe Vera has active biological compounds

that it abilities antibacterial. And in the past, in some

different countries used live of Microorganisms in foods

for human health. Recently, researchers pay attention to

foods identify that it contains Microorganisms Probiotics

such as Lactobacillus. In the present study, Aloe Vera and

Lactobacillus fermentum from a local cheese (Kozeh) used

antimicrobial material. Antimicrobial effects this material

carried out method of Agar spread and Diffusion Disk

according to Klebsiella standard and minimum inhibitor

concentration of each material got measured. The results

showed Aloe Vera extract has fewerantimicrobialeffect

upon Klebsiella bacteria, but after 72 hours culture, we

saw Supernatant lactobacillus fermentum has more

antimicrobial effect on Klebsiella. And when we use

extract and Supernatant together, we can see synergy

effect. Also it observed, this material have minimum

inhibitor concentration Klebsiella bacteria. But it didn’t

have minimum bactericidal concentration.

Keyword: Watery extract Aloe Vera, Lactobacillus

Fermentum, Cheese (Kozeh), Synergy effect, Klebsiella

The effect of probiotic Lactobacillus on the

control of weight and on serum leptin and

adiponectin status in streptozotocin-induced

diabetic rats

Alireza Dehnad1, 2, 3, Rokhsareh Ghaderi2, Maryam Taghavi Narmi 2*

1 Biotechnology Department, East Azerbaijan Research and Education

Center Agricultural and Natural Resources, AREEO, Tabriz, Iran 2 Higher Education Institute of Rab-Rashid, Tabriz, Iran 3 Research Center of Infectious and Tropical Illnesses of Medical

Sciences University, Tabriz, Iran * Corresponding author: [email protected]

Diabetes is of the most prevalence glands illnesses

throughout the world which happens at the result of a

deficiency of insulin secretion or insulin action or

sometimes both of them. Adiponectin and leptin are

proteins secreted from adipose tissue and influence on

some of the effective factors in diabetes including fatness

and insulin resistance. Probiotic bacteria are of the dietary

supplements that have useful effects on human health. The

main purpose of this research was to investigate the effects

of probiotic lactobacillus on weight control, leptin and

adiponectin status in streptozotocin-induced diabetic rats.

In this research 32 male rats of Wistar race were divided

randomly into 4 groups of 8 sets. First group: normal

control, which was fed only by standard food and 0/2 ml

phosphate-buffered saline daily. Second group: normal

control with lactobacillus that received 109cfu/ml

lactobacillus by gavage and standard food daily. Third

group: the diabetic group (using streptozotocin) that were

fed only by 30 g of fatty food daily. Fourth group: diabetic

group with lactobacillus received 109cfu/ml lactobacillus

by gavage in addition to fatty food diet. After the treatment

period (6 weeks), a liquid profile in the blood was

measured. The results showed that probiotic lactobacillus

in diabetic rats with lactobacillus in proportion to diabetic

control, could decrease the serum level of leptin

significantly (p<0.05) and increase the serum level of

adiponectin and rats’ weight significantly (p<0,05). By

considering the results, it was defined that the probiotic

lactobacillus can influence adiponectin and leptin and also

the overweight caused by diabetes.

Keywords: Lactobacillus, Probiotic, Leptin, Adiponectin,

Diabetes


114

Anticoccidial effect of metabolites of native

Streptomyces on prevalent eimeria of Iran

Alireza Dehnad1, 2, Maryam Taghavi Narmi2* 1 Biotechnology Department, East Azerbaijan Research and Education Center Agricultural and Natural Resources, AREEO, Tabriz, Iran 2 Higher Education Institute of Rab-Rashid, Tabriz, Iran


The aim of this study is the isolation and characterization

of anticoccidial metabolite producing Streptomyces sp.

from Azerbaijan, Iran. Primary screening of the seven

strains by TLC and reagent guided to select a novel

Streptomyces sp. 6. The antibacterial susceptibility of

crude extracts against pathogen microorganisms was

assessed by agar disc diffusion method and broth

microdilution method. Based on data analysis, HPLC at

the 280 nm wavelength at the flow rate of 0.5 ml/min, in

the C18 column was used for 2 isolates 6 and 8 which

isolate 6 shows peaks as salinomycin antibiotics. Then it

was used for OPG test on eimeriosis chickens. It lowered

morbidity/mortality rate, decreased oocysts per gram of

feces. Collectively, these data demonstrated the potential

of isolate 6 to control chicken coccidiosis. Isolate 6 can,

therefore, probably be used as an effective means to

control coccidiosis. According to the results Improvement

of cell culture, media and metabolite production in

suspension culture and more can be suggested.

Keywords: Anticoccidial, Metabolites, Streptomyces,

Eimeria

Histopathological evaluation of liver and

spleen after immunized mice with

recombinant PilQ, b-flagellin vaccine

Narges Bagheripour, Iraj Nikokar*, Sobhan Faezi, Seyedeh Golchereh

Mirlahiji, Soheila Rasooly, Sima Bakhtazad

Medical Biotechnology Research Center, Paramedicine Faculty, Guilan

University of Medical Sciences, Rasht, Iran


Pseudomonas aeruginosa is an opportunistic pathogen that

caused nosocomial infection in compromised patients. The

aim of this study was to determine the histopathological

changes in the liver and spleen of infected mice after

treatment with recombinant PilQ and type b-flagellin

antibodies in the burn mouse model. In the present study,

the BALB/c mice were infected by Pseudomonas

aeruginosa and then treated with antibodies raised against

PilQ and type b-flagellin in different groups. The mice

were sacrificed and the spleen and liver were aseptically

removed. For investigation of the histopathological

changes, the Hematoxylin and Eosin staining was

performed. Studies showed that when mice were treated

with anti-PilQ and type b-flagellin antibodies, reducing

necrosis and hemorrhage, the reduction of inflammatory

cells among hepatocyte cells and concentrations around

the central vein was observed. In this group, there was an

increase in megakaryocyte and decreased inflammatory

cells and low changes in the red and white pulp of the

spleen. In the tissue sections of the treated group with

Pseudomonas aeruginosa suspension, changes in the white

and red pulp occurred, and also inflammatory cells were

increased compared to test groups. These results showed

that co-administration of antibodies raised against

recombinant PilQ and b-flagellin in the challenged mice

could make less histopathological changes in the liver and

spleen of mice compared to other treated groups.

Keywords: Pseudomonas aeruginosa, PilQ, b-flagellin,

Nosocomial infection

115

Immuno-magnetic diagnosisof Brucella

abortus based on of iron and graphene

nanoparticles

Hamid Raza Taheri1, Mojtaba Salouti1, Bahram Amini2*, Reza Shapouri1,

Arash Shams1, Mohsen Kalantari1 1 Department of Biology, Faculty of Sciences, Zanjan Branch, Islamic

Azad University, Zanjan, Iran 2 Department of Biology, Science and Research Branch (IAU), Islamic Azad University, Tehran, Iran

* Correspondingauthor: [email protected]

Brucella is one of the common diseases between livestock

human, which is transmitted to human by livestock. There

are many common methods for diagnosing Brucella. But

these methods don’t have requisite sensitivity and

efficiency. In this study,the iron nanoparticles (MNPs) and

graphene nanoparticles(GQD) were synthesized.Poly-

clonal antibodies against Brucella (800 μgmL-1, 5μL)

associated with Nano think acid-probe and 50mg of GQD

were conjugated using EDC and NHS linkers. GQD were

separated by centrifugation at 8000 rpm, for10 min. Then,

the GQDswere washed 3 times with distilled

water.Next;MNPs (30mg) were conjugated to antibody by

EDC and NHS linkers.The MNP-IgG was separated by

magnetic field.Subsequently, the MNP-IgGwere washed 3

times with distilled water. For determining the sensitivity

of the method, MNP-IgG (3 mg) and GQDs-IgG(5mg)

were added to the different concentrations of Brucella

(101-108CFU mL-1) at the LB-broth medium for 45 min.

The MNP-IgG-Brucella-IgG-GQDs Complex was

separated by magnetic field. In order to release the signal

probe, the complex was dissolved in 1 mL of0.5M DTT

solution at 65 °C. The supernatant was separated and then

was read by fluorescencespectrophotometry. The specific

of the method was checked at the presence of the different

species of the bacteria. Theresults showed that the size of

the synthesized MNPand GQDswere 23-35nm and 205-

242 nm,respectively. The sensitivity of the method was

determined as 102 CFU mL-1.

Keywords:Brucella, MNP-IgG, GQDs, Immuno-magnetic

Isolation and identification of nitrogen-fixing

rhizospheric bacteria from Narcissus flower

Seyyed Mansour Seyyed Nejad1, Hossein Motamedi1, 2 *, Zinat Zirrahi1 1 Department of Biology, Faculty of Science, Shahid Chamran University of Ahvaz, Ahvaz, Iran 2 Biotechnology and Biological Science Research Center, Shahid

Chamran University of Ahvaz, Ahvaz, Iran * Corresponding author: [email protected]

Narcissus flower is one of the economic agricultural

products of Behbahan in Khouzestan province that is

marketed as a cut flower and also as a valuable source for

essential oil production. Hence this increasing the yield of

this flower is of significant economic importance. Using

plant growth promoting bacteria is a suitable approach for

this purpose. Nitrogen fixation is one of the mechanisms

that is used by these bacteria and promote the growth of

the plant. Nitrogen is a growth limiting nutrient for plant

growth and yield and in contrast to this fact that it is one of

the most elements in soil, only a small fraction of it is

available for plant growth. The aim of this study was to

isolation and identification of nitrogen-fixing bacteria from

the rhizosphere of Narcissus. In order to isolate

rhizospheric bacteria, 13 soil samples were collected from

the root of Narcissus flower in Behbahan city. 10 g of soil

was dispersed in 90 ml of physiological saline and stirred

for one hour. The supernatant was then cultured on

nutrient agar medium and incubated at 30 °C for 48 h. The

isolates were screened for nitrogen fixation according to

Dobereiner method and identified based on the enzymatic

and biochemical analysis. Separated bacteria were cultured

on a Dobereiner medium to determine nitrogen fixation

capability. As a result, from 37 bacterial isolates, 28

isolates have nitrogen fixation capability that belongs to

the genus Bacillus and Pseudomonas. The use of nitrogen

fixation bacteria isolated from the rhizosphere of Narcissus

flower can improve nitrogen uptake and subsequently

increase plant growth and yield. These isolates can also be

a good alternative to chemical fertilizers.

Keywords: Nitrogen fixation, Rhizosphere, Narcissus

flower



116

Evaluation of antibacterial activity of

methanolic extract of the polypore fungus

Phellinus sp.isolated from Mazandaran

forests

Samaneh Chaharmiri Dokhaharani1*, Masoomeh Ghobad-Nejhad1, Abbas

Farazmand1, Hamid Moghimi2, Hossein Rahmani3

1Department of Biotechnology, Iranian Research Organization for

Science and Technology (IROST), Tehran, Iran 2 Department of Microbial Biotechnology, School of Biology, College of Science, University of Tehran, Tehran, Iran 3 Department of Chemical Technologies, Iranian Research Organization

for Science and Technology (IROST), Tehran, Iran

*Corresponding author: [email protected]

Some polypore fungi have diverse pharmaceutical and

medical applications. Since Staphylococcus aureus and

Pseudomonas aeruginosa are major causes of hospital-

acquired infection (HAI) and raise antibiotic-resistant

isolates, the aim of this study was to assess the

antibacterial effect of the polypore fungus Phellinus sp.

methanolic extract against these bacteria. Phellinus sp. was

collected in November 2017 from Mazandaran forests.The

methanolic extract of the fruiting body of Phellinus sp.

was prepared by maceration method. Then the antibacterial

activity of the extract was evaluated by the broth micro

dilution method against S. aureus ATCC25923 and P. aeruginosa ATCC9027. The MIC of the methanolic

extract of Phellinus sp. against S. aureus and P.

aeruginosa were 0.7, 1.5 mg/ml, and the MBC of that were

6.25, 12.5 mg/ml. The results of this study showed that

methanolic extract of Phellinus sp. has antibacterial

activity. It is concluded that the studied polypore fungus

may be a source of antibacterial agent and more studies

about its antibacterial properties is required.

Keywords: Antibacterial, Phellinus sp., Polypore fungus

Antibacterial activity of Stachys persica from

Labiatae

Leila Joudi*

Department of Agriculture and Animal Science, Shabestar Branch, Islamic Azad University, Shabestar, Iran


The extract or essential oils of some plants show strong

antimicrobial activities and could be used as antimicrobial

drugs or food preservation. Many spices of Labiatae

family are aromatic plants, which have large amounts of

volatile oils and frequently show antimicrobial properties. In this research antimicrobial activity of essential oil and

water extract, ethanol extract, acetone extract, methyl-

ethyl-ether extract and toluene extract of Stachys persica

(Labiatae), were studied on Staphylococcus aureus in

vitro. Disk diffusion method used todetermining inhibitory

caused by the effect of the plant extract essential oils on

bacteria. The results of experiments demonstrated

that water extract of the plant was not effective on the

microorganism. Ethanol and acetone extracts hadn’t

antibacterial activities. In contrast,methyl ethyl ether and

toluene extracts showed a significant antibacterial effect.

Among the extracts, toluene extract had the strongest

effect, and then methyl ethyl ketone was effective. Also,

the volatile oil of S. persica on S. aureus had a strong

effect on the microorganism and essential oil was more

effective than extracts.

Keywords: Stachys persica, Essential oil, Extract, Disk

blank


117

Isolation and identification of an Actinomycete

isolate capable of producing anti-MRSA

compound from soil samples and optimization

of production in liquid culture

Maryam Nooshadokht1*, Bagher Amirheidari2, Maryam Shamsadini2

1 Department Pathobiology, Faculty of Veterinary, University of Shahid Bahonar Kerman, Iran 2 Department Pharmaceutical Biotechnology, Faculty of Pharmacy,

University of Medical scienceof Kerman, Iran * Corresponding author: [email protected]

The increasing resistance to commonly used antibiotics

has become a major public health concern. This

phenomenon can lead to higher treatment costs and length

of stay in the hospital. Purpose of this study Streptomycess

trains isolated from soil samples of native Morphological

and biochemical basis of their inhibition effect against

MRSA, high chosen the isolation, Therefore, further

molecular identification and optimization of the production

of antibiotic substances. Ten strains of actinomycetes

based on morphological characteristics and uses a semi-

differential medium were isolated and purified. Three field

isolates identified by morphological characteristics,

biochemical and molecular (16S rDNA) was performed.

Drug Susceptibility Testing of MRSA cases were

antibiotic disk diffusion method than 6. Antagonistic effect

of Dvmtdpen isolated-Linear and agar well diffution were

used. Four solvent extraction method of antibiotic liquid-

liquid used and the dried extract wasre-dissolved, and agar

well diffution method was investigated. Three antagonistic

actinomycetes against MRSA isolates showed that the

growth inhibitory effect of two strains of considerable

value. The most effective antibiotic isolated from cultures

obtained on the seventh day of the etherextract showed the

best effect. Ten isolates identified by conventional

methods and by molecular methods and PCR amplification

of 16S rDNA sequencing and comparison with gene bank

was completed. The soil can bean important source of

actinomycetes produce antibiotics against organisms that

are resistant. Researchers believe that such a plan because

the country needs to obtain new sources of bacteria to

antibioticsis necessary.

Keywords: PCR, 16S rDNA, Streptomyces, Methicillin-

resistant Staphylococcus

Prevalence of coagulase positive

Staphylococcus pseudintermedius in some

domestic animals

Mojtaba Mohseni1, Bahar Malek1*, Rahem Khoshbakht2

1 Department Microbiology, Faculty of Science, University of Mazandaran, Babolsar, Iran 2 Department Pathobiology, Faculty of Veterinary Medicine, Amol

University of Special Modern Technologies, Amol, Iran * Corresponding author: [email protected]

Staphylococcus pseudintermedius is a new staphylococcal

species which has been recognized as an opportunistic

pathogen in many kinds of domestic animals, especially in

dogs and cats. In recent years, the occurrence of

methicillin resistance in Staphylococcus pseudintermedius

has increased significantly. Isolation and prevalence of

coagulase positive S. pseudintermedius in some domestic

animals was the aim of the current study. In order to

isolate the S. pseudintermedius, clinical specimens were

taken by a sterile swab from the snouts and noses of

domestic animals including dogs, cats and their owners

who referred to veterinary clinics of Mazandaran province.

The specimens were directly inoculated on mannitol salt

agar and incubated at 37°C for 48h. Staphylococci were

identified by their morphological and physiological

characteristics. In addition, oxacillin screening plate

method was used for determination of methicillin-resistant

isolates. The results of this study showed that in 65 clinical

specimens, 54 isolates were Staphylococcus. Moreover,

the results of the tube coagulase test showed that 11

isolates were coagulase positive. These results

demonstrated that the prevalence ofcoagulase positive S.

pseudintermediusin clinical specimens collected from

dogs, cats and pet owners were 12.3, 3.02 and 1.5 percent,

respectively. This study indicated that coagulase positive

S. pseudintermedius was present in domestic dogs and

cats. Due to the constant interaction between these animals

and their owners, it is important to prevent the

transmission of antibiotic-resistant staphylococci between

animals and humans. Achieved data were assessed by

SPSS software and KHI 2 test. P<0.05 was designated as

the meaningful level.

Keywords: Staphylococcus pseudintermedius, Coagulase,

Domestic animals

118

Study and evaluation of antibacterial

properties of clove essential oil

Masoomeh Ghadimi1, Hamideh Vaghari1,2*, Mohammad Javad Najian2,

Yahya Najian2, Hoda Jafarizadeh Malmiri1

1 Faculty of Chemical Engineering, Sahand University of Technology,

East Azarbaijan, Tabriz, Iran. 2 Research and Development Department, Najian Herbal Group, East Azarbaijan, Tabriz, Iran.


Medicinal herbs have long been used traditionally for

treatment of human diseases and recent scientific and

technology advances have made much more importance

and role of medicinal herbs in meeting human needs,

especially in the field of medicine and treatment. These

include the role of medicinal plants in the treatment and

prevention of gum disease and dental caries. Streptococcus

mutans is known as one of the most important etiologic

factors in plaque formation and tooth decay. Streptococcus

mutans is a spherical gram-positive bacterium, have a

capsule, produce acid and remain stable at the mucosal

surfaces exposed to saliva flow, with colony formation and

replication. Clove as an important plant is an effective herb

in ancient and modern medicine, and the essential oil of

Clove has a significant anti-bacterial effect. In this study,

the effect of Clove on Streptococcus mutans, dental caries

was investigated. So that the distillate and essential oil of

dried Clove blooms were prepared using a clevenger

apparatus through distillation with water and steam.

Antimicrobial activity of Clove distillate and essential oil

against Streptococcus mutans was investigated using an

antimicrobial laboratory method of agar well diffusion

method. In this method, plate surface containing nutrient

agar is inoculated by spreading a volume of the microbial

inoculum over the entire agar surface. Then, a hole with a

diameter of 6 mm is punched aseptically using gel puncher

and a volume (20 µL) of the antimicrobial agent solution is

introduced into the well. Then, agar plates are incubated at

37°C for 24h. The antimicrobial agent diffuses in the agar

medium and inhibits the growth of the microbial strain

tested. Based on the results, the distillate from Clove

flowers did not have an antibacterial effect on

Streptococcus mutans. In contrast, Clove essential oil

showed a significant effect on inhibiting the growth of the

bacteria and the inhibition zones in some repetitions was

obtained about 22 to 31 mm in average.

Keywords: Clove, essential oil, antibacterial property,

inhibition zone


119

Authors Index

A Abasalt Hosseinzadeh Colagar .......................... 3, 9, 64, 88

Abbas Farazmand .......................................................... 116

Abolfazl Ardjmand .......................................................... 97

Abolfazl Barzegar ........................................................... 93

Abolfazl Daneshvar ........................................................... 6

Abolfazl Jafari-Sales ............................................. 106, 107

Adeleh Divsalar ............................................... 6, 52, 55, 84

Ahad Mokhtarzadeh ...................................................... 110

Ahmad Aghaee ................................................................ 93

Ahmad Farhad Talebi .................................................. 6, 10

Aida Jalili Bolhasani ....................................................... 44

Aida Mohamad Amoie .................................................... 91

Alam Ara Gholami ........................................................ 105

Ali Abedinzadeh Geshlagi............................................... 77

Ali asghar Ahmadi .................................................... 96, 97

Ali Bahadori .......................................................... 103, 104

Ali Foroutan .................................................................... 47

Ali Mohammadi .............................................................. 63

Ali Movafeghi ................................................................. 85

Ali Sadighi ............................................................ 103, 104

Ali Soleimani .................................................................. 99

Ali Taravati ....................................................28, 29, 30, 35

Alireza Dehnad ...................................................... 113, 114

Alireza Diba .................................................................. 109

Alireza Iranbakhsh .......................................................... 55

Alireza Panahi ................................................................. 56

Alireza Tarinejad ....................................................... 57, 58

Aliyeh Daryanavard ........................................................ 42

Amin Ramezani ............................................................... 89

Amir Abbas Barzegari ................................................... 102

Amir Arasteh ................................................................... 95

Amir Charkaneh .............................................................. 76

Amir Ekhtiyari ................................................................ 96

Amir Hossain Ahmadi ..................................................... 92

Amir Jalali ......................................................................... 6

Amir Tukmechi ............................................................. 113

Amirarsalan Kavyanifard .............................................. 112

Arash Kianianmomeni ..................................................... 85

Arash Shams .................................................................. 115

Arefeh Seyedarabi ........................................................... 84

Arman Mahmoudi Otaghvari ............................................ 6

Arman Rostamzad ................................................... 12, 110

Asadollah Mohammadi ..................................38, 39, 84, 86

Ashraf Asadi .................................................................... 73

Ashraf Shahvelayati ........................................................ 48

Atfeh Iranmanesh .......................................................... 108

Atieh Gheisari ................................................................. 52

Ayub Karimzadeh ........................................................... 49

Azadeh Ebrahim Habibi .................................................. 21

Azadeh Hekmat ................................................... 43, 52, 55

Azadeh Kazemi ......................................................... 96, 97

Azam Afaghi ............................................................. 66, 67

AzarAbassi .................................................................... 110

Azita Dilmaghani .......................................................... 111

B Babak Elyasifar .............................................................. 111

Bagher Amirheidari ....................................................... 117

Bahar Malek ................................................................... 117

Bahram Amini .................................................... 45, 46, 115

Behboud Jafari ............................................................... 106

Behnam Mortazavi ........................................................... 81

Behrang Alani .................................................................. 97

Behrouz Nasseri ............................................................... 48

Boshra Mohammadi Ahmadvandi ............................. 34, 35

D Daniel E. Otzen ................................................................ 37

Dina Morshedi ........................................................... 37, 79

Donya Fahmi .................................................................... 66

E Ebrahim Valipour............................................................. 65

Ehsan Azin ....................................................................... 13

Ehsan Dehnavi ................................................................. 90

Ehsan Ghodrati Shatori .................................................... 83

Ehsan Seyedjafari............................................................. 59

Elaheh Zadeh Hosseingholi ....................................... 22, 64

Elham Ahmadi ................................................................. 58

Elham Behruz .................................................................. 75

Elham Bidabadi .............................................. 60, 68, 69, 70

Elham Elahifard ............................................................... 80

Elham Hajian Kelarijani .................................................. 35

Elham Mohajel Kazemi ..................................................... 6

Elham Ojaghi ................................................................. 100

Elham Rajabbeigi ............................................................... 6

Elham Rezvannejad ......................................................... 87

Elham Siasi ...................................................................... 12

Emaduddin Moudi ........................................................... 88

Esmaeil Babaei ................................................................ 11

Esmail Doustkhah ............................................................ 48

F Faezeh Dehghani Esmatabad ........................................... 79

Faezeh Ghanati .................................................................. 6

Fahimeh Alizadeh .......................................................... 109

Fahimeh Feyzie Sani ...................................................... 103

Farah Farahani ........................................................... 66, 67

Farahnaz Asadi ................................................................ 31

Farahnaz Dashbolaghi ...................................................... 40

Faramarz Mehrnejad ........................................................ 64

Farank Mohammad Ashoori ...................................... 53, 54

Farhad Mashayekhi ........................................ 60, 68, 69, 70

Farhang Aliakbari ...................................................... 37, 79

Fariba Khodagholi ........................................................... 26

Fariba Mahmoudi ............................................................. 56

Farid Heidari .................................................................... 81

Farideh Mohammad Hossein Zadeh .............................. 107

Farideh Razi ..................................................................... 21

Farideh Zayermashhad Bonab ......................................... 14

Farnaz Rasi-Bonab ................................................. 106, 107

Farnoosh Khosrobakhsh ................................................... 88

Farokh Karimi .................................................................. 61

120

Farrokh Karimi ................................................ 4, 62, 78, 79

Farshad Darvishi ........................................................... 4, 6

Farshid Zandsalimi ........................................................ 112

Farzad Nazari .................................................................... 6

Farzam Ajamian .............................................................. 76

Farzane Kargar .............................................................. 103

Farzaneh Hosseini ......................................................... 101

Farzaneh Hossieni ........................................................... 12

Fateme Hajian ................................................................. 99

Fateme Zahra Rezanejad Keshteli ................................... 91

Fatemeh Abdollahi ............................................................ 6

Fatemeh Golshahi ............................................................ 34

Fatemeh Khani-Habibabadi ............................................. 94

Fatemeh Mousavi ............................................................ 31

Fatemeh Nejadhabibvash .................................................. 6

Fatemeh Notghi Oskui .................................................... 14

Fatemeh Rajabi Dehnavi ........................................... 70, 71

Fatemeh Tohidi ............................................................... 30

Fereshteh Dadfar ......................................................... 6, 50

Fereshteh Jozaghkar ........................................................ 88

Fereshteh Motamedi ........................................................ 26

Fereshteh Ramezani Khorsand ........................................ 90

Firozeh Bordbar ............................................................ 108

Forough Sanjarian ........................................................... 53

G Ghazal Khooshehchin ..................................................... 21

Ghazale Dini .................................................................... 65

Ghazaleh Farhadi............................................................. 69

Gholam Reza Mahdavinia ............................................... 40

Gholamreza Dehghan .................................5, 25, 26, 44, 45

Gholamreza Gohari ........................................................... 6

Gholamreza Zarrini ........................................................... 7

H Habibollah Mahmoodzadeh .............................................. 9

Hadi Ansarihadipour ................................................... 6, 41

Hadis Shahbazi .................................................................. 6

Haedeh Mobaiyen ......................................................... 106

Haleh Homayoni ............................................................. 55

Hamed Hasanpour ......................................................... 104

Hamid Azizi .................................................................... 49

Hamid Moghimi ............................................................ 116

Hamid Raza Taheri........................................................ 115

Hamid Rezanezhad .......................................................... 91

Hamid Taghavi Dehaghani .............................................. 48

Hamid Zahednasab ............................................................ 9

Hamideh Lamakan .......................................................... 98

Hamideh Vaghari .......................................................... 118

Hamzeh Amiri ................................................................... 6

Hanieh Babaei ................................................................. 23

Hanieh Mohajjel Shoja ...................................................... 6

Hanif Yaghoobi ............................................................... 11

Hassan Aghdasinia .......................................................... 14

Hero Ghafaryan ............................................................... 32

Hoda Jafarizadeh Malmiri ............................................. 118

Hojat Mohammadnia ....................................................... 47

Hosein Jamalifar ............................................................ 108

Hossain Riahi ................................................................ 105

Hossein Ghafouri............................................38, 39, 84, 86

Hossein Honari .......................................................... 10, 89

Hossein Mohammad-Beigi .............................................. 37

Hossein Motamedi ......................................................... 115

Hossein Naderi-Manesh ................................................... 87

Hossein Nahrevanian ....................................................... 23

Hossein Rahmani ........................................................... 116

Hossein Soltanzadeh ............................................ 74, 75, 86

Hossein Tayefi Nasrabadi ................................................ 33

Houman Maftoon ............................................................. 28

I Ilker Buyuk ................................................................ 71, 72

Iraj Nikokar .................................................................... 114

J Jafar Razeghi .................................................................... 85

Jamal Hallajzadeh ............................................................ 99

Jamshid Khan Chamani ....................................... 49, 50, 51

Jamshid Mehrvand ..................................................... 45, 46

Javad Najafeian ................................................................ 12

Javad Zahiri ...................................................................... 87

Javid Rezaei ..................................................................... 68

K Kambiz Larijani ............................................................... 43

Kamiar Khadivi .............................................................. 104

Kamran Ahmadi ............................................................... 56

Kamyyar Khadivi ........................................................... 103

Karim Hasanpur ............................................................... 85

Karim Sorkheh ........................................................... 71, 72

Kataneh Abrari ................................................................. 54

Katayoon Nofouzi ............................................................ 33

Kazem Mahdigholi............................................................. 6

Kazem Parivar .................................................................. 91

Khadijeh Razavi ................................................................. 6

Khalil Maleki Chollu ............................................. 103, 104

Khalil Maleki Chulu ...................................................... 104

Khosro Mehdi-Khanlo ............................................... 71, 72

Khosrow Khalifeh ...................................................... 46, 73

Kiyanoush Bakhshande .................................................... 29

Kosar Sokri .................................................................... 100

Kourosh Bamdad ......................................................... 6, 50

L Ladan Shakoorzadeh Ardebili .......................................... 51

Leila Alizade .................................................................... 58

Leila Hasani ..................................................................... 46

Leila Joudi ..................................................................... 116

Leila Nezhaddadghar ....................................................... 56

Leila Sadeghi ................................................................... 30

Leila Zarandi-Miandoab .................................................. 22

Leili Hasheminejad ........................................................ 103

Leyla Hashemi Nezhad .................................................. 104

Lida Momeni .................................................................... 27

M Maede Moradi .................................................................. 40

Mahboobeh Talebi Mehrdar............................................. 21

Mahboubeh Kaviani ...................................................... 83

Mahboubeh Mehmankhah................................................ 81

Mahdi Emdadi ................................................................ 102

Mahdi Hasan Shahian .................................................... 108

Mahdi Hosseinzadeh .................................................. 10, 89

121

Mahdi Rahnama ........................................................ 69, 75

Mahdi Zeinoddini ............................................................ 90

Mahdie Farvandi ............................................................. 38

Mahdieh Daneshjo........................................................... 95

Mahdieh Farvandi ........................................................... 84

Mahmood Maleki .......................................................... 103

Mahmoud Vesal .............................................................. 89

Mahmoudreza Aghamaali ......................................... 39, 83

Mahnazsadegi Shabestari ................................................ 92

Mahnoush Mohammadzadeh Shahir ............................... 67

Mahsa Abedini ................................................................ 70

Mahsa Tarighi ................................................................. 80

Mahtab Razlansari ............................................................. 7

Majid Jadidi ..................................................................... 54

Majid Motovali-Bashi ............................................... 70, 71

Majid Parak ....................................................................... 6

Majid Rajabiian ............................................................... 27

Majid Tafrihi ................................................................... 57

Maliheh Sadat Atri .............................................. 34, 35, 40

Mansour Ghaffari Moghaddam ....................................... 47

Marjan Rahimi .............................................................. 103

Maryam Ghobeh ........................................................ 23, 31

Maryam Hatami............................................................... 87

Maryam Hemmati ............................................................. 5

Maryam Hosseinnia................................................... 72, 73

Maryam Jokar .................................................................. 37

Maryam Mehrabi ............................................................. 41

Maryam Mirbagheri ...................................................... 109

Maryam Mohadjerani ................................................ 35, 40

Maryam Mohajerani ............................................ 29, 34, 35

Maryam Monsef Shokri ............................................ 31, 43

Maryam Nooshadokht ................................................... 117

Maryam Rashki ............................................................... 77

Maryam Sadat Daneshpour ............................................. 23

Maryam Salavatifar ..................................................... 6, 76

Maryam Shamsadini ...................................................... 117

Maryam Taghavi Narmi ........................................ 113, 114

Marzieh sadat Ahmadi .................................................... 59

Masomeh Babaee ............................................................ 36

Masomeh Khalili ............................................................. 60

Masomeh Tartifi ........................................................ 71, 72

Masood Hashemzaei ..................................................... 102

Masoomeh Ghadimi ...................................................... 118

Masoomeh Ghobad-Nejhad ........................................... 116

Masoud Taheri .............................................................. 107

Masoud Torkzadeh Mahani ........................................... 103

Masoumeh Asl Rousta .................................................... 68

Masoumeh Hasannezhad ............................................... 102

Masoumeh Moradi Moghadami .................................... 101

Masoumeh Valipour .................................................. 43, 44

Mehdi Abbasnejad......................................................... 107

Mehdi Eskandarian .......................................................... 79

Mehdi Imani .................................................................... 42

Mehdi Koushki .............................................................. 106

Mehdi Moslemi ............................................................... 26

Mehdi Rahimpour ........................................................... 94

Mehran Mesgari Abbasi .................................................. 33

Mehrdad Behmanesh ................................................. 92, 94

Mehrdad Payandeh........................................................... 59

Mehrdad Rostami ............................................................. 99

Mehrdad Zahiri-Tous ....................................................... 59

Mina Owrang ................................................................. 105

Mina Taheri .................................................................... 110

Mina Zafarpiran ................................................... 73, 74, 78

Mir Kamyar Musavi ....................................................... 101

Mir Naghi Fazeli Ghadi ................................................. 105

Mohadeseh Ghaffari ......................................................... 98

Mohadesseh Asadi ........................................................... 13

Mohamad Moghadam ...................................................... 90

Mohamad Pourhasan ...................................................... 104

Mohamad Reza Alivand .................................................. 86

Mohamad Reza Fazeli .................................................... 108

Mohammad Ali Hoseinpour Feizi .................................... 14

Mohammad Ali Hosseinpour Feizi .................................. 77

Mohammad Ali Mohammadalizadeh ............................... 54

Mohammad Ali Zarei ........................................... 32, 36, 37

Mohammad Farkhari ........................................................ 80

Mohammad Javad Najian............................................... 118

Mohammad Khalaj-kondori ................................. 73, 74, 78

Mohammad Khalaj-Kondori ...................................... 77, 80

Mohammad Mohammadzadeh ......................................... 63

Mohammad Pooya Naghshbandi ................................... 111

Mohammad Rahmati Yamchi .......................................... 58

Mohammad Rahmati-Yamchi .......................................... 93

Mohammad reza Alivand ........................................... 78, 79

Mohammad Reza Alivand ............................................... 75

Mohammad Reza Mashayekhi ................................... 80, 96

Mohammad Reza Saberi ...................................... 49, 50, 51

Mohammad Sattari ..................................................... 53, 54

Mohammad Shokrzadeh ............................................ 96, 97

Mohammad Taghi Ghorbanian ........................................ 54

Mohammad Yousefi ......................................................... 52

Mohammad Zarei ............................................................. 55

Mohammadali Hosseinporfeizi ........................................ 78

Mohammadreza Nahaei ................................................. 110

Mohhamad hossein Pourfeizi ........................................... 92

Mohsen Kalantari ........................................................... 115

Mohsen Mobini ................................................................ 94

Mohsen Sagha .................................................................. 63

Moj Khaleghi ................................................................. 108

Mojtaba Mohseni ................................................. 6, 13, 117

Mojtaba Mortazavi ................................................... 87, 103

Mojtaba Mortezavi ........................................................... 77

Mojtaba Norouzi .............................................................. 53

Mojtaba Salouti .............................................................. 115

Mona Hassanzadeh .......................................................... 93

Monire Khordadmeh ........................................................ 33

Morahem Ashengroph ....................................................... 6

Moslem Afsharnezhad ..................................... 5, 24, 27, 36

Mostafa Norizadeh Tazehkand ...................................... 6, 8

Mostafa Shakhsi Niaie ..................................................... 94

Mostafa Shourian ................................................. 32, 84, 86

Mouj Khaleghi ............................................................... 107

Mousa Bohlooli ................................................................ 47

Mozhdeh Torkzaban ...................................................... 111

Mozhgan Mohammad Hosseinzadeh Noghondari ..... 49, 50

122

N Nabat Naqshbandi ........................................................... 27

Nader Chaparzadeh ......................................................... 64

Nadia Ramezani ............................................................ 111

Naeemeh Kazemzadeh .................................................. 102

Naeimeh Roshanzamir .................................................... 82

Nafisseh Farazandehnia ................................................. 108

Nahid Askari ..................................................................... 6

Nahid Shahabadi ............................................................... 7

Najaf Allahyari Fard ........................................................ 81

Narges Alipour Saqa ....................................................... 48

Narges Bagheripour ....................................................... 114

Narges Nikonahad ........................................................... 83

Naser Jafargholizadeh ..................................................... 60

Nasim Golestannejad ....................................................... 84

Nasim Hayati Roodbari ................................................... 46

Nasrin Ahmadpour .................................................... 45, 46

Nasrin Amiri Dashatan .................................................. 106

Nasrin Fattahi ................................................................ 109

Nasrin Kazempour........................................................... 22

Nasrollah Soori .............................................................. 112

Nastaran Monzavi ............................................................. 7

Navvabeh Salarizadeh ..................................................... 47

Nayebali Ahmadi........................................................... 106

Nazaila Valatabar ............................................................ 92

Nazanin Abedinzadeh ................................................... 105

Neda Karami ................................................................... 13

Neda Neghabi .................................................................. 64

Neda Poormolaie ............................................................. 47

Negin Bermeh ................................................................. 80

Nemat Sokhandan Bashir ................................................ 11

Nematolah Gheibi ............................................................. 7

Nematollah Razmi ........................................................... 23

Niloufar Ghayoumipour .................................................. 36

Nima Shaykh-Baygloo ...................................................... 6

O Orkideh Hajipour............................................................... 6

P Parastoo Erfanmanesh ............................................... 56, 99

Parichehreh Yaghmaei .................................................... 21

Parisa Fathi Rezaei .......................................................... 48

Parisa Malekpour............................................................. 86

Parizad Azami ............................................................... 104

Parvaneh Maghami .................................................... 43, 44

Parviz Abdolmalaki ................................................... 53, 54

R Raana Hasanlo ................................................................. 75

Raheleh Ebrahimi .............................................................. 6

Raheleh Hasanzadeh ....................................................... 39

Raheleh Majdani ....................................................... 77, 98

Rahem Khoshbakht ....................................................... 117

Ramin Soleimani ............................................................. 42

Raoof Jahan-Bakhsh .......................................................... 9

Reyhaneh Sariri ............................................... 5, 24, 27, 36

Reza Alihemmati ........................................................... 102

Reza H.Sajedi ............................................................ 10, 90

Reza Hassan Sajedi ......................................................... 47

Reza Javan ....................................................................... 69

Reza Khakvar ........................................................... 11, 100

Reza Masoomi Jahandizi ......................................... 59, 101

Reza Mohammadzadeh .................................................... 63

Reza Safaralizadeh ........................................................... 92

Reza Shapouri ................................................................ 115

Reza Sotoudeh ................................................................. 87

Reza Valipour .................................................................. 65

Reza Vojdani .................................................................... 89

Reza Yekta ............................................................. 5, 25, 26

Robabe Narimani ............................................................. 74

Robabeh Soleimani .......................................................... 76

Roghayyeh Baghban ........................................................ 93

Rokhsareh Ghaderi......................................................... 113

Roya Boroumand Gohar .................................................. 43

Roya Hatefirad ................................................................. 98

Roya Salari Moghadam .............................................. 28, 29

Roya Salehi ...................................................................... 58

S S. Kazem Sabbagh ........................................................... 83

S. Shirin Shahangian ............................ 5, 24, 27, 36, 39, 83

S. Shohreh Mirmortazavi ........................................... 38, 86

Saba Sherkat Khabazi ................................................ 57, 58

Saba Yari.......................................................................... 59

Saber Khodabandeh ......................................................... 42

Saber Raeghi .................................................................... 99

Saber Zahri ................................................................. 49, 56

Sadegh Moradi ................................................................. 97

Saeed Irian ....................................................................... 84

Saeede Heydarzadeh ...................................................... 113

Saeid Amirian-ghatar ....................................................... 78

Saeid Mohebbi ................................................................. 62

Safa Lotfi ......................................................................... 87

Safar Farajnia ................................................................... 93

Saleh shahabivand ............................................................ 93

Saleh Shahabivand ............................................................. 3

Saman Mahdavi ............................................................. 102

Samaneh Chaharmiri Dokhaharani ................................ 116

Samaneh Ghasemali ......................................................... 93

Samaneh Heydarzadeh ............................................... 78, 79

Samaneh Montazery ......................................................... 55

Samaneh Rashtbari..................................... 5, 25, 26, 44, 45

Samaneh Sarrafzadeh ..................................................... 102

Samira Aslani ................................................................... 39

Samira Shahbazi................................................................. 6

Samira Shahbazy .............................................................. 53

Sanaz Mahmazi .................................................... 68, 69, 75

Sara Ghaffarian .................................................................. 6

Sara Mola ali abasiyan ..................................................... 40

Seifollah Bahramikia ....................................................... 34

Sevil Nematollahi........................................................... 100

Seyed Alireza Mesbah-Namin ........................................... 9

Seyed Jalal Zargar .................................................. 7, 59, 60

Seyed Mohammad Atyabi .......................................... 66, 67

Seyed Yahya Salehi-Lisar .................................................. 6

Seyede Saramohsenizade ................................................. 88

Seyede Tahere Mir-Salehi ................................................ 30

Seyedeh Golchereh Mirlahiji ......................................... 114

Seyedeh Maral Marashi ................................................... 92

123

Seyedeh Maryam Ekrami ................................................ 13

Seyed-Morteza Javadirad .......................................... 70, 71

Seyyed Mansour Seyyed Nejad ..................................... 115

Shadi Babaei .................................................................... 68

Shadi Farahmand ............................................................. 95

Shahin Ehtesham Far ....................................................... 28

Shahryar Shakeri ........................................................... 103

Shahrzad Askari .............................................................. 94

Shirin Shahangian ........................................................... 47

Shiva Khalil- Moghaddam .............................................. 48

Shohreh Mohamadi ......................................................... 41

Shokoufe Rezaei .............................................................. 10

Siamak Asri Rezayi ......................................................... 29

Sima Bakhtazad ............................................................. 114

Simin Khatayi ............................................................ 44, 45

Sina Mehrpooyan ............................................................ 79

Sobhan Faezi ................................................................. 114

Soheila Ebrahimi ............................................................... 6

Soheila Rasooly ............................................................. 114

Soheila Shir Mohammadi ................................................ 23

Sohrab Pajnameh ............................................................. 11

Solin Ghader .................................................................... 33

Solin Ghaderi .................................................................. 33

Somayeh Keypour ......................................................... 105

Soraya Mohammadzadeh .......................................... 45, 46

Soroush Soltani ............................................................... 48

Soulmaz Ekhtari .............................................................. 85

Souraia Mohammadzadeh ............................................... 46

Syed Naqui Kazim .......................................................... 81

T Taghi Lashkarblouki ....................................................... 54

Tahereh Zahedi .................................................................. 9

V Vahab Jafarian ................................................45, 46, 72, 73

Vahid Niknam .................................................................... 6

Vahid Yousefi Babadi ...................................................... 30

Vahideh Hasanzadeh ........................................................ 82

Vajiheh Eskandari .............................................................. 8

Valilollah Babaeipour ...................................................... 10

Y Yahya Najian ................................................................. 118

Younes Aftabi .................................................................. 64

Yousef Abdossalami ........................................................ 31

Yousef Lotfi Hadi Beyglu .............................................. 104

Yousef Lotfi Hadi Biglu ................................................ 103

Yousef Mohammadi ................................................... 95, 96

Yousef Shahali ................................................................. 31

Yunske Ide ....................................................................... 48

Z Zahra Abedi kichi ............................................................ 92

Zahra Barati Shourijeh ............................................... 89, 90

Zahra Faghih .................................................................... 89

Zahra Hajihassan ............................................................ 112

Zahra Mortazavi ............................................................... 50

Zahra Noormohammadi ............................................. 66, 67

Zahra Rezaei .................................................................... 10

Zahra Roshani .................................................................. 52

Zarrin Minuchehr ............................................................. 81

Zeinab Fadaei ................................................................... 27

Zeinab Rusta .................................................................... 98

Zeinab Soleimani sardo .................................................... 32

Ziba Babazadeh fardi ..................................................... 111

Zibasadat Majid Seyed biglou .......................................... 12

Zinat Zirrahi ................................................................... 115

Zivar Salehi ...................................................................... 76

Zohreh Jahanafrooz .......................................................... 65

Zohreh Taheri ............................................................ 61, 63

Zohreh Toghranegar ........................................................... 6

Proceeding of Cellular and Molecular Biology...

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