Membrane Separation

Process

Background of Membrane separation

technology

• Simple filtration technology which can separate

molecules according to their molecular size

• No heat treatment→ Low Initial Cost, Small Energy, → Low Initial Cost, Small Energy,

High Quality of Products

Name of membranes Molecular weight

Pore size

Permeate flux of water at 38 bar,24°С (LMH)

Å Ångström nanometer

UM 05UM 2UM 10

5001 000

10 000

212430

2.12.43.0

1734

102UM 10PM 10PM 30XM 50XM 100AXM 300

10 00010 00030 00050 000

100 000300 000

30384766

110480

3.03.84.76.6

11.048.0

102935850425

11052215

Objective of the Work

Development of a membrane separation

process which can efficiently purify anserine-process which can efficiently purify anserine-

carnosine (AC) extracted from chicken meat

Materials

(preparation of chicken extract)

Chicken carcass

Extraction

Water

water:carcasses=3:1

100˚C, 4-6 h

Ion exchange resin

NF membrane

Removal AC: 6.25 g/L (235)

Creatinine: 2.31 g/L (113)

Na+: 0.27 g/L (56)Acidic and neutral

amino acids

Apparatus

Lab-Module type 20.(DSS)

Maximum membrane area:

7200 cm2 (180 cm2×40 sheets)Maximum pressure: 6 MPaMaximum pressure: 6 MPa

Maximum temperature: 100˚C

pH-range: 0-14

Membranes used in this study

MembraneNaCl rejection(*MW cut-off)

Manufacturer Material

NFT50 55 DSS Polypiperazine /polyamide/

DRA4510 45 DAISEN Polyamide

Desal DL 15 Desalination Polyamide (aromatic)

Desal DK 50 Desalination Polyamide (aromatic)

NTR7430 30 NITTO DENKO Sulfonated polyether sulfone

NTR7450 50 NITTO DENKO Sulfonated polyether sulfone

NTR7250 60 NITTO DENKO Polyvinyl alcohol

MPF34 35 Abcor Polysulfone

MPF36 10 Abcor Polysulfone

MPF44 25 Abcor Polyacrylonitrile (PAN)

MPF50 700* Abcor Polyacrylonitrile (PAN)

G-5 1000* Desalination Polyamide

G-10 2500* Desalination Polyamide

Total Circulation Experiment

Feed

PI

Ret

enta

te

F

I

PI

Feed flow rate: 5.8 - 11.3 L/minPressure: 1 - 6 MPaTemperature: 25 ˚C

FeedRet

enta

te

Permeate

Pump

Feed tank

Batch Wise Experiment

Permeate

Ret

enta

te PI

FI

PI

Creatinine and Sodium Ion

Area of membrane: 360 cm2

Temperature: 25 ˚CInitial weight of Feed: 11.2 kgFinal weight of Feed: 3 kg

Ret

enta

te

Feed

Pump

Feed tank

Analyses and Calculation of Rejection Value

� HPLC using column (TSKG2, 500PWXl) with 45% acetonitrile (pH3.0).

� ICP-AES (JICP-PS3000UV; Leeman Lab).

.. b C� .. b

Robs: Observed rejectionCp: Concentration of permeateCr: Concentration of retentate

r

p

obsC

CR −=1

Results of Total circulation experiment

Effect of operating pressure (�P) on permeate flux (Jv) and

observed rejection for anserine-carnosine (RAC), creatinine (RCr)

and sodium ion (RNa) with NFT50 membrane

Results of Total circulation experiment

Effect of flow rate on permeate flux (Jv) and observed

rejection for anserine-carnosine (RAC), creatinine (RCr) and

sodium ion (RNa) with NFT50 membrane

Result of total circulation experiment

Membrane Jv x106 [m3/(m2 s)] RAC RCr RNa

NFT50 61.1 0.998 0.765 0.811

DRA4510 54.9 0.994 0.813 0.835

Desal DK 42.4 0.992 0.713 0.733

Desal DL 36.8 0.997 0.439 0.446

MPF36 34.7 0.751 0.490 0.257MPF36 34.7 0.751 0.490 0.257

NTR7250 29.2 0.888 0.564 0.234

MPF50 28.5 0.017 0.035 -

NTR7430 27.8 0.925 0.600 0.719

NTR7450 13.9 0.941 0.704 0.842

MPF34 11.8 1.000 0.990 0.980

G-10 8.8 0.453 0.214 0.588

MPF44 6.3 0.940 0.886 0.757

G-5 4.6 0.406 0.070 0.593

Results (Batch-Wise Concentration)

Changes in purity, yield, concentration of anserine-carnosine and

permeate flux value (Jv) during Batch wise experiment with NFT50

membrane (flow rate:10 L/min, pressure:4 MPa)

Proposal of a Mathematical Model

A: membrane area

Jv: Permeate flux

V: volume of solution

t: time

C: concentration of components)1(

)(ACacv

fACRCJA

dt

VCd−−=

ρv

JAdt

dV−= (1)

(2)

C: concentration of components

R: rejection of components

CF: concentration factor

D: constant

k: mass transfer coefficient

dt

)ln(CFkDJv −=

fV

VCF 0=

(3)

(4)

Simulation Results

Changes in purity, yield, concentration (CAC) of anserine-carnosine

and permeate flux value (Jv) with NFT50 membrane (experimental

value and calculation line)

Process design

Chicken carcass

Extraction

Ion exchange resin

5 t/d

4-6 h

RemovalEluted solution 1,400 kg

Water 15 t

Extract 15,000 kg

NF membrane

Anserine-Carnosine

Creatinine and sodium ion

Acidic and neutral

amino acids NFT50 membrane

1.2 m2, 6 hPermeate

Eluted solution 1,400 kg

Retentate: 150 kg

(conc.: 7.5%, purity: 90%, yield: 98%, AC: 11.2 kg)

Whey

Purification /centryfuge and cake filter/

UF membraneRetentate

Permeate

Permeate

3 kind of UF

membrane

Curd and

Retentate

NF membrane

Lactulose

Solvent

Solvent protein’s 3 kind of NF

membranePermeate

RetentatePermeate

Retentate

(purity: 90%, yield: 95%)

membrane

Thank you very much for

your kind attention