Presentasi Camag DKI

8/4/2019 Presentasi Camag DKI

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Planar Chromatography


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What you need:

a method TLC or HPTLC precoated

layers

suitable equipment

know how

Planar Chromatography...


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A method...

Developed andvalidated byCAMAG



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CAMAG Laboratory


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www.camag.com


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Download methods for free...

www.camag-laboratory.com


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What you need :

a method

TLC or HPTLC precoated

layers suitable equipment

know how



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TLC/HPTLC precoated layers

available at CAMAG or atyour lab supplier



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TLC vs. HPTLC TLC HPTLC

Mean particle size: 10 - 15 m 5 - 7 m

Particle distribution: wide narrow

Layer thickness: 250 m 100, 200 m

Number of samples: max. 12 36 - 72

Migration distance: 100 - 150 mm 30 - 70 mm

Migration time: 30 - 200 min 3 - 20 minSolvent use: 50 ml 5 - 10 ml

Detection limit: Abs 100 - 1000 ng 10 - 100 ng

Fluor 1 - 100 ng 0,1 - 10 ng


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Comparison TLC-HPTLC

Flavonoids (Ph.Eur.4)

HPTLC plate 20 x 10 cm7 cm migration

10 mL solvent

10 min.

TLC plate 20 x 20 cm

12 cm migration

25 mL solvent

32 min.


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What you need:

a method

TLC or HPTLC precoated


know how



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Sample application

Chromatogram development

Detection & quantitative evaluation

Documentation

Post-Chrom. Derivatization


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Sample application is the mostcritical step inthe TLC procedure.

Errors which occur during sample application,cannot be compensated for in chromatogramdevelopment or evaluation.

Sample application


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step by step

Sample Application



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Sample Application

Nanomat

volume volume volume

1L

Linomat

Spray-on technique

2L 1L 2L 10L5L

Spot versus Band application


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Sample application - semi automatic

LINOMAT5

Sprays on samples as narrow bands

Larger volumes can be applied (1 - 200 L)

Precise sampling of programmed volumes Sample application for:

qualitative

quantitative

preparative separation

LINOMAT


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Linomat 5

Keypad & Display

Plate holderTurret with dosing syringe


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Sample application: fully automatic ATS4

PC - controlled

Application of spots, bands or rectangles

Reduced time for application

Improved reproducibility

GLP compliant

Suitable for danger substances


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Automatic TLC Sampler 4

DisplayRack for sample vialsor micro-titer plates

Turret with dosing syringe

Protection cover Waste bottleRinsing bottle

Plate holder


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Automatic TLC Sampler 4

Contact transfer = Spots

Spray-on = Bands

Spray-on = rectangular


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step by step

Sample Application Chromatogram Development

Evaluation

Documentation



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Chromatogram development

Fully automatic gradient separation via AMD2

Manual separation via Glass tank

Automatic isocratic separation via ADC


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ADC2

Chromatogram developmentwith standardized conditions

in a conventional glass tank fully automatic

saturation controlled

humidity controlled

unsurpassed reproducible

qualifiable IQ/OQ


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TLC -sprayer


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step by step


Evaluation

Documentation



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Visual inspection in Viewing box


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Densitometry

TLC Scanner 3


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Scanning the chromatogram

Integrated track


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Densitometry


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Spectrum Scan

Display of the selected spectra


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Spectrum LibraryAnalysis spectrum compared

with a spectrum from the library


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Detection multi wavelength scan

A selection of up to

31 WL is possible


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Classical densitometry Multi Wavelength Scan

One track at all wavelengths


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step by step


Evaluation

Documentation



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TLC VISUALIZER:

Documentation, Evaluation, Archiving


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TLC Visualizer Design has been enhanced to further improve the

homogeneity of the illumination of the plate:

254 nm, short wave UV - Reflectance

366 nm, long wave UV - Reflectance

White light - Reflectance and/orTransmission.

Ergonomic design: Easy access in to the illuminationcompartment via large sliding door.

separate viewing window with UV-protection

Timer for automatic switch-off of the light sources


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Documentation system TLC VISUALIZER


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Manual Optimization

If desired, a region of interest can be defined, in which the brightestspot is automatically found.


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RF-Tool to measure RF of the substances

winCATS with TLC Visualizer


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is GLP/GMP compliant:

All significant parameters of

a TLC method are recordedand electronically stored.



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What you need:

a method TLC or HPTLC precoated


know how



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Know How...

Application LabTraining courses

individual Training



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Why CAMAG?

> 40 years of experience

satisfied customers

individual solutions

competent After Sales Service

training courses



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CAMAG worldwide CAMAG Muttenz / Switzerland

www.camag.ch

CAMAG China Supporting Unit Hong Kong & Guangzhouwww.camag-china.com

CAMAG Berlin / Germanywww.camag-berlin.de

CAMAG Scientific Inc. North Carolina / USA

www.camagusa.com

CAMAG Regional Support Unit Bombay / India

www.hptlc-anchrom.in


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..... no laboratory is

complete without amodern HPTLC system!

Conclusion ...


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Thanks for your attention !

Presentasi Camag DKI

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