Practical courses

PLANT PHYSIOLOGY

http://kfrserver.natur.cuni.cz

1. Conduct yourself in a responsible manner at all times in the laboratory.

2. Follow all written and verbal instructions carefully. If you do not understand a direction or part of a procedure, ASK YOUR TEACHER BEFORE PROCEEDING WITH THE ACTIVITY.

3. Do not eat food, drink beverages, or chew gum in the laboratory. Do not use laboratory glassware as containers for food or beverages.

4. Observe good housekeeping practices.Work areas should be kept clean and tidy at all times.

5. Dispose of all chemical waste properly. Never mix chemicals in sink drains. Sinks are to be used only for water. Check with your teacher for disposal of chemicals and solutions.

6. Know the locations and operating procedures of all safety equipment including: first aid kit(s), and fire extinguisher. Know where the fire alarm and the exits are located.

Laboratory safety rules

Topic:

PLANT PIGMENTSPlastid •••• Vacuolar

Why plants are green?

Buchanan et al. 2000

Taiz a Zeiger 2002

Photosynthetic pigments - chlorophyls- carotenoids

Ross Koning -Plant Physiology Information Website.http://plantphys.info/index.html

Ross Koning -Plant Physiology Information Website.http://plantphys.info/index.html

Light harvesting complex

Taiz a Zeiger 2002

Carotenoids – protective function

Taiz a Zeiger 2002

Transport of e- and H+ on the thylacoid membrane

Today:

1a) Extract membrane-bound pigments from the leaves of ivy-berry (Hedera helix)

1b) What is the ability of extracted pigments to absorbe the light, and mediate the transfer of electrons from thedonor to the acceptor molecule.

Plastid pigments

Vacuolar pigmentsflavonoids -anthocyans, flavons, flavonols

Buchanan et al. 2000

Vacuolar PigmentsFlavonoids - antocyans, flavons, flavonols

Taiz a Zeiger 2002

Experiment (2)

Vacuolar pigments

2a) Separate flavonoids bypaper chromatography. According to thetable try to identify what kinds of flavonoids are onthe chromatograme.

Fig.2: Scheme of paper chromatography

Experiments:

1a) Prepare an extract of plastid pigments from Hedera helix leaves

1b) Try to reduce a dye (methyl red) byan electron transported from a donor (ascobic aacid) by means of the pigmentextract.

Plastid pigments

β-carotene

violaxanthin

chlorophyll a

chlorophyll b

pheophytin

lutein

neoxanthin