Polymerase chain reaction (PCR) يلسلستلا ةرملبلا لعا فت · PDF...

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Transcript of Polymerase chain reaction (PCR) يلسلستلا ةرملبلا لعا فت · PDF...

Page 1: Polymerase chain reaction (PCR) يلسلستلا ةرملبلا لعا فت · PDF filePolymerase chain reaction (PCR) ... The PCR reaction will amplify the DNA section between the
Page 2: Polymerase chain reaction (PCR) يلسلستلا ةرملبلا لعا فت · PDF filePolymerase chain reaction (PCR) ... The PCR reaction will amplify the DNA section between the

Polymerase chain reaction (PCR)تف اعل البلمرة التسلسلي

المحاضرة الثامنة

طلة المللي. د

Page 3: Polymerase chain reaction (PCR) يلسلستلا ةرملبلا لعا فت · PDF filePolymerase chain reaction (PCR) ... The PCR reaction will amplify the DNA section between the
Page 4: Polymerase chain reaction (PCR) يلسلستلا ةرملبلا لعا فت · PDF filePolymerase chain reaction (PCR) ... The PCR reaction will amplify the DNA section between the
Page 5: Polymerase chain reaction (PCR) يلسلستلا ةرملبلا لعا فت · PDF filePolymerase chain reaction (PCR) ... The PCR reaction will amplify the DNA section between the
Page 6: Polymerase chain reaction (PCR) يلسلستلا ةرملبلا لعا فت · PDF filePolymerase chain reaction (PCR) ... The PCR reaction will amplify the DNA section between the
Page 7: Polymerase chain reaction (PCR) يلسلستلا ةرملبلا لعا فت · PDF filePolymerase chain reaction (PCR) ... The PCR reaction will amplify the DNA section between the

DNA templateقالب الدنا : أوال

Page 8: Polymerase chain reaction (PCR) يلسلستلا ةرملبلا لعا فت · PDF filePolymerase chain reaction (PCR) ... The PCR reaction will amplify the DNA section between the
Page 9: Polymerase chain reaction (PCR) يلسلستلا ةرملبلا لعا فت · PDF filePolymerase chain reaction (PCR) ... The PCR reaction will amplify the DNA section between the
Page 10: Polymerase chain reaction (PCR) يلسلستلا ةرملبلا لعا فت · PDF filePolymerase chain reaction (PCR) ... The PCR reaction will amplify the DNA section between the
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dATP, dCTP, dGTP, dTTPتحوي على •

: النكليوتيد•أساس آزوتي+ مجموعة فوسفاتية(+ بالنسبة للدنا)هو سكر خماسي ديوكسي ريبوز

Page 12: Polymerase chain reaction (PCR) يلسلستلا ةرملبلا لعا فت · PDF filePolymerase chain reaction (PCR) ... The PCR reaction will amplify the DNA section between the
Page 13: Polymerase chain reaction (PCR) يلسلستلا ةرملبلا لعا فت · PDF filePolymerase chain reaction (PCR) ... The PCR reaction will amplify the DNA section between the

DNA Polymerase: رابعا

Page 14: Polymerase chain reaction (PCR) يلسلستلا ةرملبلا لعا فت · PDF filePolymerase chain reaction (PCR) ... The PCR reaction will amplify the DNA section between the

Primersالبادئات: خامسا

Page 15: Polymerase chain reaction (PCR) يلسلستلا ةرملبلا لعا فت · PDF filePolymerase chain reaction (PCR) ... The PCR reaction will amplify the DNA section between the

كيف يتم التفاعل؟

Page 16: Polymerase chain reaction (PCR) يلسلستلا ةرملبلا لعا فت · PDF filePolymerase chain reaction (PCR) ... The PCR reaction will amplify the DNA section between the
Page 17: Polymerase chain reaction (PCR) يلسلستلا ةرملبلا لعا فت · PDF filePolymerase chain reaction (PCR) ... The PCR reaction will amplify the DNA section between the

األخرى

Page 18: Polymerase chain reaction (PCR) يلسلستلا ةرملبلا لعا فت · PDF filePolymerase chain reaction (PCR) ... The PCR reaction will amplify the DNA section between the
Page 19: Polymerase chain reaction (PCR) يلسلستلا ةرملبلا لعا فت · PDF filePolymerase chain reaction (PCR) ... The PCR reaction will amplify the DNA section between the
Page 20: Polymerase chain reaction (PCR) يلسلستلا ةرملبلا لعا فت · PDF filePolymerase chain reaction (PCR) ... The PCR reaction will amplify the DNA section between the
Page 21: Polymerase chain reaction (PCR) يلسلستلا ةرملبلا لعا فت · PDF filePolymerase chain reaction (PCR) ... The PCR reaction will amplify the DNA section between the
Page 22: Polymerase chain reaction (PCR) يلسلستلا ةرملبلا لعا فت · PDF filePolymerase chain reaction (PCR) ... The PCR reaction will amplify the DNA section between the
Page 23: Polymerase chain reaction (PCR) يلسلستلا ةرملبلا لعا فت · PDF filePolymerase chain reaction (PCR) ... The PCR reaction will amplify the DNA section between the
Page 24: Polymerase chain reaction (PCR) يلسلستلا ةرملبلا لعا فت · PDF filePolymerase chain reaction (PCR) ... The PCR reaction will amplify the DNA section between the
Page 25: Polymerase chain reaction (PCR) يلسلستلا ةرملبلا لعا فت · PDF filePolymerase chain reaction (PCR) ... The PCR reaction will amplify the DNA section between the
Page 26: Polymerase chain reaction (PCR) يلسلستلا ةرملبلا لعا فت · PDF filePolymerase chain reaction (PCR) ... The PCR reaction will amplify the DNA section between the
Page 27: Polymerase chain reaction (PCR) يلسلستلا ةرملبلا لعا فت · PDF filePolymerase chain reaction (PCR) ... The PCR reaction will amplify the DNA section between the

Gel electrophoresis

Page 28: Polymerase chain reaction (PCR) يلسلستلا ةرملبلا لعا فت · PDF filePolymerase chain reaction (PCR) ... The PCR reaction will amplify the DNA section between the
Page 29: Polymerase chain reaction (PCR) يلسلستلا ةرملبلا لعا فت · PDF filePolymerase chain reaction (PCR) ... The PCR reaction will amplify the DNA section between the
Page 30: Polymerase chain reaction (PCR) يلسلستلا ةرملبلا لعا فت · PDF filePolymerase chain reaction (PCR) ... The PCR reaction will amplify the DNA section between the
Page 31: Polymerase chain reaction (PCR) يلسلستلا ةرملبلا لعا فت · PDF filePolymerase chain reaction (PCR) ... The PCR reaction will amplify the DNA section between the

هنتغتون

Page 32: Polymerase chain reaction (PCR) يلسلستلا ةرملبلا لعا فت · PDF filePolymerase chain reaction (PCR) ... The PCR reaction will amplify the DNA section between the

هنتغتون

هنتغتون

هنتغتون

Page 33: Polymerase chain reaction (PCR) يلسلستلا ةرملبلا لعا فت · PDF filePolymerase chain reaction (PCR) ... The PCR reaction will amplify the DNA section between the

هنتغتون

Page 34: Polymerase chain reaction (PCR) يلسلستلا ةرملبلا لعا فت · PDF filePolymerase chain reaction (PCR) ... The PCR reaction will amplify the DNA section between the
Page 35: Polymerase chain reaction (PCR) يلسلستلا ةرملبلا لعا فت · PDF filePolymerase chain reaction (PCR) ... The PCR reaction will amplify the DNA section between the
Page 36: Polymerase chain reaction (PCR) يلسلستلا ةرملبلا لعا فت · PDF filePolymerase chain reaction (PCR) ... The PCR reaction will amplify the DNA section between the
Page 37: Polymerase chain reaction (PCR) يلسلستلا ةرملبلا لعا فت · PDF filePolymerase chain reaction (PCR) ... The PCR reaction will amplify the DNA section between the

DNA fingerprinting البصمة الوراثية

Page 38: Polymerase chain reaction (PCR) يلسلستلا ةرملبلا لعا فت · PDF filePolymerase chain reaction (PCR) ... The PCR reaction will amplify the DNA section between the
Page 39: Polymerase chain reaction (PCR) يلسلستلا ةرملبلا لعا فت · PDF filePolymerase chain reaction (PCR) ... The PCR reaction will amplify the DNA section between the

Polymerase Chain Reaction (Pcr) k

Polymerase Chain Reaction (Pcr) k

3. PCR Polimerase Chain Reaction

3. PCR Polimerase Chain Reaction

Chain reaction: 30 years of PCR

Chain reaction: 30 years of PCR

Polymerase Chain Reaction: Types, Utilities and Limitations · Polymerase Chain Reaction: Types, Utilities and Limitation s 161 1.4 Reverse Transcriptase PCR (RT-PCR) This PCR was

Polymerase Chain Reaction: Types, Utilities and Limitations · Polymerase Chain Reaction: Types, Utilities and Limitation s 161 1.4 Reverse Transcriptase PCR (RT-PCR) This PCR was

Polymerase Chain Reaction (PCR) - ITK diagnostics bv Polymerase... · Polymerase Chain Reaction (PCR) ... DNA polymerase, dNTPs, MgCl2 and reaction buffers are provided at optimized

Polymerase Chain Reaction (PCR) - ITK diagnostics bv Polymerase... · Polymerase Chain Reaction (PCR) ... DNA polymerase, dNTPs, MgCl2 and reaction buffers are provided at optimized

POLYMERASE CHAIN REACTION - Medical Genetics · PDF file06/06/2002 · Contents Polymerase Chain Reaction PCR Reaction Components Standard PCR Reaction Avoiding Contamination

POLYMERASE CHAIN REACTION - Medical Genetics · PDF file06/06/2002 · Contents Polymerase Chain Reaction PCR Reaction Components Standard PCR Reaction Avoiding Contamination

Polymerase chain reaction PCR - humsc.net

Polymerase chain reaction PCR - humsc.net

REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION RT-PCR€¦ · REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION RT-PCR Tünde Szatmári, PhD NRIRR, Budapest, 2016 . RT-PCR AND TRADITIONAL

REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION RT-PCR€¦ · REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION RT-PCR Tünde Szatmári, PhD NRIRR, Budapest, 2016 . RT-PCR AND TRADITIONAL

Analysis of Xbp-mRNA RT-PCR. Polymerase Chain Reaction (PCR)

Analysis of Xbp-mRNA RT-PCR. Polymerase Chain Reaction (PCR)

PCR - San Diego Miramar Collegefaculty.sdmiramar.edu/bhaidar/bhaidar 210A web uploads/Course... · • Setting‐up a PCR reaction ... PCR: polymerase Chain Reaction ... Microsoft

PCR - San Diego Miramar Collegefaculty.sdmiramar.edu/bhaidar/bhaidar 210A web uploads/Course... · • Setting‐up a PCR reaction ... PCR: polymerase Chain Reaction ... Microsoft

Polymerase Chain Reaction ( PCR ) - utoledo.edu

Polymerase Chain Reaction ( PCR ) - utoledo.edu

Polymerase Chain Reaction (PCR)

Polymerase Chain Reaction (PCR)

Metode PCR (Polymerase Chain Reaction)-1

Metode PCR (Polymerase Chain Reaction)-1

PCR (polymerase chain reaction). PCR Scelta dello stampo Scelta dei primer.

PCR (polymerase chain reaction). PCR Scelta dello stampo Scelta dei primer.

Polymerase Chain Reaction What is PCR History of PCR How PCR works Optimizing PCR Fidelity, errors & cloning PCR primer design Application of PCR.

Polymerase Chain Reaction What is PCR History of PCR How PCR works Optimizing PCR Fidelity, errors & cloning PCR primer design Application of PCR.

Inspiration. Challenge. Eppendorf. · 2018-02-15 · Microarrays Real-time PCR cyclers PCR reaction plates PCRR reaction plates PPC reaction Objectives ... as industrial companies

Inspiration. Challenge. Eppendorf. · 2018-02-15 · Microarrays Real-time PCR cyclers PCR reaction plates PCRR reaction plates PPC reaction Objectives ... as industrial companies

The polymerase chain reaction (PCR). Experiment Goals Understand how PCR technique works. Perform PCR experiment. Analyze PCR products.

The polymerase chain reaction (PCR). Experiment Goals Understand how PCR technique works. Perform PCR experiment. Analyze PCR products.

PCR quantitativo. What is Real-Time PCR? Real-Time PCR is a specialized technique that allows a PCR reaction to be visualized “in real time” as the reaction.

PCR quantitativo. What is Real-Time PCR? Real-Time PCR is a specialized technique that allows a PCR reaction to be visualized “in real time” as the reaction.

Polymerase Chain Reaction - PCR

Polymerase Chain Reaction - PCR

POLYMERASE CHAIN REACTION - dhiantika.staff.ugm.ac.iddhiantika.staff.ugm.ac.id/files/2011/04/PCR-LDW.pdfPolymerase Chain Reaction Tehnik PCR merupakan metode amplifikasi (penggandaan)

POLYMERASE CHAIN REACTION - dhiantika.staff.ugm.ac.iddhiantika.staff.ugm.ac.id/files/2011/04/PCR-LDW.pdfPolymerase Chain Reaction Tehnik PCR merupakan metode amplifikasi (penggandaan)