PLASTOX WP 3: MPs!uptake!and!food!web!transfer · Maria1 Grannberg4, Susanne1 Kuhen 3,1 Rocco1...
Transcript of PLASTOX WP 3: MPs!uptake!and!food!web!transfer · Maria1 Grannberg4, Susanne1 Kuhen 3,1 Rocco1...
Project info
This project has received funding iunder the framework of JPI Oceans
Project logo
Project info
Acknowledgement of relevant funding agencies. Please find all logo’s on the following website: https://epss-jpi-oceans.ptj.de/partners
Project logo
3.5 3.8 4.1 4.4 4.7 5.0 5.3 5.6
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Cl/biphe
nyl
Incuba/on Time (weeks)
MPs-‐PCBs in sediment
PE PET PS PP PVC
3.5 3.8 4.1 4.4 4.7 5.0 5.3 5.6
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Cl/biphe
nyl
Incuba/on Time (weeks)
MPs-‐PCBs in water
PE PET PS PP PVC
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-‐ Clear inges;on of PS and GF -‐ Possible preferen;al inges;on of PS. -‐ No evidence of excre;on.
Fig. 8: Reduc;ve dechlorina;on of PCB (Aroclor 1254) adsorbed to different MPs in sediment (A) and water (B) PCB-‐dechlorina;ng marine cultures.
Microplas;cs (MPs) are increasingly documented in marine organisms, including species involved in human consump;on such as molluscs, crustaceans and fishes. Physiochemical proper;es (size, density, shape, chemical composi;on) of MPs might influence their inges;on by organisms and subsequent food web transfer. Most knowledge is from laboratory work, and there is li[le empirical informa;on on the distribu;on and detec;on of MPs in nature. The aims of the work are to: • Quan;fy and characterise MPs
occurrence in key marine species with different feeding strategies from relevant coastal ecosystems around Europe.
• Iden;fy relevant spa;al distribu;on pa[erns of MPs
• Understand which proper;es tend to favour MPs inges;on and reten;on by the organisms
PLASTOX is supported by national funding agencies in the framework of JPI Oceans and other institutions
PLASTOX WP 3: MPs uptake and food web transfer
• The presence of MPs in our organisms was consistently rather small compared to previous data from the literature.
• The
Organisms from different trohic levels were processed for MPs extrac/on, quan/fica/on and characteriza/on (µ-‐FTIR).
h[ps://www.sintef.no/projectweb/plastox/
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Background and aims
Field-‐based and laboratory studies at sta/ons represen/ng a wide range of European marine environments (Adria/c, North Sea, Atlan/c Ocean) and habitats (saltmarshes, sedimentary coastal habitats and pelagic coastal systems)
MPs in food webs_ field studies
Location B
Location C
Site 1 Site 2 Site 3
Site 1 Site 2 Site 3
Site 1 Site 2 Site 3
C.galucum
M.galloprovincialis
C.aestuarii
Nereis
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Baiona Bellocchio Chioggia
Average of M
Ps/organ
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1Alma Mater Studiorum -‐ University of Bologna, Italy; ; 2 FCT-‐NOVA, Portugal; 3IMARES Wageningen UR, Netherlands; 4 IVL Swedish Environmental Research Ins;tute, Sweden; 5 Ghent University, Belgium; 6Na;onal University of Ireland Galway; 7Norwegian University for Science and Technology; 8SINTEF Materials and Chemistry, Norway.
Stefania Piarulli1, Laura Airoldi1, Filipa Bessa2, Paolo Comandini1, Thomas Doyle6, Marco Capolupo1, Elena Fabbri1, Jan van Franeker3, Maria Grannberg4, Susanne Kuhen3, Rocco Mazzeo1, Kers/n Magnusson4, Silvia Pra/1, Antonella Rosato1, Lurgi Salaverria7, Sara Scapinello1, Giorgia Sciu^o1, Paula Sobral2, Carl Van Colen5, Brecht Vanhove5, Alina Madita Wieczorek6, Joanne XW Wong1,Giulio Zanaroli1, Andy Booth8
Location A
Uptake & accumula/on_lab experiments
PE
PS
GF -‐ MP concentra;on: 75, 750 & 7500 par;cles mL-‐1 -‐ Exposure ;me: O. marina: ≤1 h; C. finmarchicus: ≤96 h -‐ Sta;c exposure on custom made plankton wheels
PCB-‐dechlorina/ng marine cultures -‐ Incuba;on in sediment vs. water -‐ PCB-‐contaminated MPs (Aroclor 1254 30 mgPCBs/kgMP) vs. pris;ne MPs
MPs in marine food webs Experimental approaches
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Diplodus vulgaris Dicentrarchus labrax
Platichthys flesus Mic
ropl
astic
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y (%
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Uptake & accumula/on Oxyrrhis marina
Calanus finmarchicus
• Adults and nauplii ingested all MP types.
• MPs excreted in faecal pellets.
• Faecal pellet produc;on reduced in the absence of algae.
• MPs may be retained for prolonged period.
PCB-‐dechlorina/ng marine cultures
-‐ The presence of MPs consistently rather small compared to previous data. -‐ The majority of MPs extracted were fibres par;cularly in invertebrates species. -‐ Higher presence of MPs in organisms from areas with stronger anthropogenic ac;vi;es -‐ Very high variability in the MPs gastrointes;nal contents among individuals
Fig 1: Red circles indicates european marine stations where field and laboratory studies have been performed.
Fig 2: Experimental design for organisms collection along the trophic chain.
A B C
Fig 3: (A) organism dissection; (B) digestion solution; (C) Filters (20 µm) with inorganic and synte;c par;cles aker filtra;on
Fig 4: Commercially available microplas;cs:
PS (10 µm) PE (3-‐16 µm) Fluorescent (GF; 1-‐µm)
Fig. 5: Microcosms with MPs pellets inoculated with PCB-‐dechlorina;ng culture enriched from a marine sediment
Fig. 7:(A-‐B) P and fluorecent GF inside Oxyrrhis marina; (C-‐D-‐E) all plas;cs pellets inside Calanus finmarchicus
A BC
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Fig 6: MPs distribu;on in different species from four european marine sta;ons: (a) Adria;c Saltmarshes; (b) North sea saltmarshes; (c) Atlan;c Ocean; (d) west-‐Sweden coastal areas; (e) MPs found in mesopelagic fishes from Atlan;c Ocean iden;fied with µ-‐FTIR.
(e)