Photofabrication of 3D Protein Hydrogels for Nanobiotechnology Applications
description
Transcript of Photofabrication of 3D Protein Hydrogels for Nanobiotechnology Applications
Photofabrication of 3D Protein Hydrogels for Nanobiotechnology Applications
BAYINDIR GROUP JOURNAL CLUB-1
Erol Özgür
MOTIVATIONS
• Microfabricated platforms in cellular biology: valuable research tools
• Microfluidics technology• Major problem is biocompatibility• Limited functionality of interfacial materials• No perturbation during experiment process is possible
• Aim: Platforms for cellular research that could be modified in situ
Protein Cross-linking for Formation of 3D Hydrogels
• BSA (Bovine Serum Albumin) is a soluble protein with 607 residues
• BSA(aq) BSA(prec.)
• UV light: Inefficient, little control on the shape
• Multiphoton excitation: Precisely controlled submicrometer sized shapes
• Photosensitizers increase the efficiency of the process
• Optical parameters:Tsunami Ti:Sapphire laser at 730-740 nm, Zeiss Axiovert IM 100X Fluar 1.3 NA objective, with a confocal scanner
hν
In Situ Formation of Protein Hydrogel
3D Protein Hydrogels Created by Photofabrication with Photomasks
Chemical Response of Protein Hydrogels: Na2SO4
Chemical Response of Protein Hydrogels: Na2SO4
Chemical Response of Protein Hydrogels: pH from 7 to 12.2 with NaOH
Microchambers with E. coli Directionality
Microvortex Created by E. coli
Axial Fluid Flow in an Arched Microchannel Produced by Flagellar Motion of E. coli
Orbital Revolution of a PMMA microdisk by E. coli
Different Strategies in Photofabrication
With Photomask and Confocal Scanner
With Digital Micromirror Device
Fabrication Process with DMD
Some Structures Fabricated with DMD
References
Kaehr, B. and J. B. Shear (2008). "Multiphoton fabrication of chemically responsive protein hydrogels for microactuation." PNAS 105(26): 8850-8854.
Kaehr, B. and J. B. Shear (2009). "High-throughput design of microfluidics based on directed bacterial motility." Lab on a Chip 9(18): 2632-2637.
Nielson, R., B. Koehr, et al. (2009). "Microreplication and Design of Biological Architectures Using Dynamic-Mask Multiphoton Lithography." Small 5(1): 120-125.