They can’t tell you what they’re allergic to That’s where we come in

Ground-breaking technology

New-generation CCD blockers

3 unique monoclonal antibodies from recombinant IgE

100% specificity with high sensitivity

One step ahead in allergy management

What are CCDs and why is it important to block them?

What is ? Next+ is an innovative allergy test for detecting specific immunoglobulin E (IgE) in the serum of dogs and cats. It has all the benefits of our ground-breaking technology it employs a new generation of CCD blockers.

Specific allergen protein Carbohydrate chains

CCDs are cross-reactive carbohydrate determinants – the carbohydrate chains found in glycoproteins. CCDs are part of the structure of many allergy-causing proteins, especially plant-based allergens like pollen. During an allergic reaction, IgE is produced against the carbohydrate chains as well as the allergen proteins. Studies have confirmed that this occurs in

30% of humans, dogs and cats [1-4].Other research shows that IgE against CCDs is clinically irrelevant. But it is now known that removing CCD-specific IgE makes in vitro test correlate well with the sensitivity and specificity of intradermal skin tests [4]. Blocking CCDs simply means that the specificity of the in vitro test is enhanced.

STEP 1 – CCD SCREENING STEP 2 – CCD BLOCKING

CCD-specific IgE Specific allergen protein CCD chain CCD-blockerAllergen-specific IgE

slgE

Relevant positive

SAMPLE 3 SAMPLE 3

Irrelevant positive

SAMPLE 4 SAMPLE 4

slgE

Relevant positive

SAMPLE 2

slgE

Relevant positive

slgE

Relevant negative

slgE

Serum

Relevant negative

SAMPLE 1

slgE

Why is the IgE test of choice?1 Three proprietary monoclonal antibodies

derived from recombinant IgE• Unique monoclonal antibodies that target

specific IgE sites• Developed from canine recombinant IgE• No cross-reactions with other

immunoglobulins like IgG• 100% specificity and superior sensitivity • Fewer in-lab steps to minimize the risk

of inaccuracy

2 New-generation semi-synthetic CCD blockers

• First test with next-generation CCD blockers • Highly specific blocking of CCDs• No interference with any tested allergens

3 Excellent accuracy and reproducibility• Best-in-class intra-assay and inter-assay

reproducibility• Daily calibration curve removes variability

IgE, IgG and BSA as antigens in differents wells

IgE

O.D

.

3,5

3

2,5

2

1,5

1

0,5

0 0,05 0,10 0,20 0,39 0,78 1,56 3,13 6,25 12,50

IgG BSA

Protein Amount [Nanogram / Well]N

orm

aliz

ed A

.U.

OD IgE curve

0 0,2 0,4 0,6 0,8 1 1,2 1,4

y = 1,1571x - 0,0116R2 = 0,9996

1,4

1,2

1

0,8

0,6

0,4

0,2

0

ALLERGENS WITHOUT CCD BLOCKER

WITH CCD BLOCKER

Phleum pratense 486 375

Poa pratensis 205 52

Dactylis glomerata 259 32

Lolium perenne 181 81

Rumex acetosella 352 52

Plantago lanceolata 316 66

Urtica spp. 205 50

Chenopodium album 358 108

Artemisia vulgaris 235 35

Ambrosia eliator 409 49

Betula pendula 428 415

Corylus avellana 247 47

Salix viminalis 100 60

Ulmus americana 121 21

Positive allergens 7 2

[1] Petersen A et al Ubiquitous structures responsible for IgE cross-reactivity between tomato fruit and grass pollen allergens J Allergy Clin Immunol 1996 Oct; 98(4):805-15

[2] Mari A et al Specific IgE to cross-reactive carbohydrate determinants strongly affect the in vitro diagnosis of allergic diseases J Allergy Clin Immunol 1999; 103(6): 1006-1011

[3] Gedon NKY et al Agreement of serum allergen test results with unblocked and blocked IgE against cross-reactive carbohydrate determinants (CCD) and intradermal test results in atopic dogs Vet Dermatol 2019; 30(3):195

[4] Álvarez J Unpublished data Alergovet S.L.

ArtuvetVijzelweg 11, 8243 PM LelystadNetherlandsinfo@artuvet.com+31 320 783 100artuvet.com

Alergovet Valentin Beato 24, 28037 MadridSpaininfo@alergovet.com+34 914 134 472alergovet.com

Dr BaddakySnickarvägen 7, 673 32 CharlottenbergSwedenpost@drbaddaky.com+46 (0)571-202 30drbaddaky.com

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One step ahead in allergy management