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Once in a while, a new product comes along that changes how people work. Fragment Analyzer ™ Automated CE System

Transcript of Once in a while, a new productsuperior instrument, superior results 8li *vekqirx %rep]^iv %yxsqexih...

Page 1: Once in a while, a new productsuperior instrument, superior results 8li *vekqirx %rep]^iv %yxsqexih ') 7]wxiq lew fiir hiwmkrih jsv ¾i\mfpi stivexmsr tvszmhmrk lmkl witevexmsr viwspyxmsr

Maximum Sample Throughput: Array dependent, either 12 or 96 samples

Maximum Unattended Sample Capacity: Up to 288 samples; unlimited with robotic arm

Minimum Sample Volume: 20 µL of liquid for injection; as little as 1 µL of actual sample required

Gel Sizing Ranges: 35-500 bp; 35-1,500 bp; 75-15,000 bp; 75 -20,000 bp

Kits Available: NGS Fragment Analysis, Genomic DNA, Total RNA, mRNA and TILLiNG

Resolution: Gel dependent; to as low as 2 bp

Sizing Accuracy: Typically 5% or better

Detection Sensitivity: As low as 5 pg/µL; 100pg/µL smear

Light Source: 1,000 mW LED, 470 nm excitation wavelength

Detector: High sensitivity CCD; 500-600 nm emission wavelength

Software: Fragment Analyzer™ instrument control, PROSize™ data analysis

Data Export Format: Microsoft® Excel® spreadsheet, binary output option

Environmental Conditions: Indoor use, normal laboratory environment 20-23°C

Relative Humidity Range: < 80% (non-condensing)

Electrical: � ��������:%'��������,^�����%��%PXIVREXI�GSR½KYVEXMSRW�EZEMPEFPI

Instrument Dimensions: � *YPP]�GSR½KYVIH����GQ�,�\����GQ�;�\����GQ�(�����\����\����MR

Instrument Weight: 38.5 Kg (85 lbs)

Advanced Analytical Technologies, Inc.2711 South Loop Drive, Suite 4150Ames, IA 50010 USAPhone: +1-515-296-6600Fax: +1-515-294-7141E-mail: [email protected]

Advanced Analytical Technologies, GmbH.Im Neuenheimer Feld 583D-69120 HeidelbergPhone: ++49 6221 868058-20Fax: ++49 6221 868058-99E-mail: [email protected]

Copyright © 2010 Advanced Analytical Technologies, Inc. All Rights Reserved. Reproduction, adaptation or translation without prior written permission is prohibited, except as allowed under copyright laws. Fragment Analyzer™ Automated CE System is a trademark of Advanced Analytical Technologies, Inc.

Once in a while, a new product comes along that changes how people work.

Fragment Analyzer™ Automated CE System

Fragment Analyzer™ Automated CE System Technical Specifications

Page 2: Once in a while, a new productsuperior instrument, superior results 8li *vekqirx %rep]^iv %yxsqexih ') 7]wxiq lew fiir hiwmkrih jsv ¾i\mfpi stivexmsr tvszmhmrk lmkl witevexmsr viwspyxmsr

SUPERIOR INSTRUMENT, SUPERIOR RESULTS8LI�*VEKQIRX�%REP]^IV��%YXSQEXIH�')�7]WXIQ�LEW�FIIR�HIWMKRIH�JSV�¾I\MFPI�STIVEXMSR��TVSZMHMRK�LMKL�WITEVEXMSR�VIWSPYXMSR� over a wide range of DNA/RNA sizes and concentrations. Different gel kits are interchangeable with the same capillary array.

Instrument CapabilitiesFragment Analyzer™ Automated CE System Technical Specifications

Typical Separation — Speed and Resolution

Fast Run Times — Separate Fragments In Under 15 Minutes

(IXIGX�776W�;MXL�'SR½HIRGI�°�(S[R�XS���FT�6IWSPYXMSR

Separate and Quantify Genomic DNA

Automate Mutation Detection Analysis Detect induced (and natural) point mutations in genomic DNA. High detection sensitivity (as low as 5 pg/µL) combined with E�WMQTPM½IH�GPIEZEKI�TVSGIWW��RS�HIWEPXMRK�WXIT�SV�)(8%� EHHMXMSR �VIWYPX�MR�XLI�IJ½GMIRX�HIXIGXMSR�SJ�QYXEXMSRW��8LI�streamlined cleavage process and fast separation times yield improved throughput over manual TILLiNG methods.

Analyze Total RNA QualityNo exchange of capillary array is required, streamlining operation and reducing costs compared to alternative methods

Other Applications

DNA and RNA Quantity and Quality Measurements

Restriction Digestion of DNA Fragments (CAPS Analysis)

NGS Fragment Library Preparation

Mixture of GeneRuler™100 bp Plus Ladder (Fermentas) plus 35 bp (lower) and 6,000 bp (upper) marker fragments

Fast Separations: Parent 1, Parent 2 and Hetero SSR samples. The DNA fragments sized as 313 bp and 423 bp corresponding to < 10% sizing error. Outer peaks represent 200 bp/700 bp markers. Migration time for 700 bp upper marker was 13 min.

DNF-900 Gel: Separation demonstrating resolution of 113 bp, 115 bp, and122 bp DNA fragments.

Genomic DNA Kit: Separation of Ѥ DNA/HindIII Marker (Promega®) with intact (D1) and degraded (B1) genomic DNA.

ADVANCED ANALYTICAL > WWW.AATI-US.COM Fragment Analyzer™ AUTOMATED CE SYSTEM ADVANCED ANALYTICAL > WWW.AATI-US.COM Fragment Analyzer™ AUTOMATED CE SYSTEM

Fragment Analyzer™ Analytical Sofware

IMPROVE THROUGHPUT BY DECREASING ANALYSIS TIME PROSize™ data analysis software is designed to simplify JVEKQIRX�MHIRXM½GEXMSR�ERH�EYXSQEXI�VSYXMRI�WGSVMRK�ERHreporting of fragments.

Flexible Data DisplayData can be displayed in electropherogram or digital gel views for any combination of rows, columns or individual samples. Overlay random wells and adjust contrast, show/hide markers, zoom/autoscale and copy to other applications.

���SV����'ETMPPEV]�%VVE]�9WEKI – Direct, parallel injectionand separation of 12 or 96 samples at once.

Short Run Times – As short as 15 min for <3,000 bp. Fast separation of 12 samples or an entire 96-well plate cycle. Get more done in less time.

High Sensitivity – Detection limits as low as 5 pg/µL without desalting, allows direct injection of PCR and digest samples.

High Separation Resolution – Resolve a wide range of DNA fragments and RNA. Achieve as low as 2 bp resolution.Custom gels can be developed.

Wide Dynamic Range – Covers 3 orders of magnitude. 0IWW�STXMQM^EXMSR�RIIHIH��IEGL�OMX�KIP�LEW�E�HI½RIH�STXMQYQ� fragment concentration range.

Low Cost per Sample – Lower operational cost compared to agarose/acrylamide gels and other analytical instruments.

User-Friendly Software – Automate data scoring. Calculate fragment size and concentration, display data in multiple formats, create and save customized scoring parameters, export and report results with ease.

Real Time Viewing of Data – See results as they are being generated, change conditions during a run if desired.

Fragment Analyzer™ Key Features and Benefits

4SSPIH�(2%��IUYMZEPIRX�SJ���HMTPSMH�SVKERMWQW ��EQTPM½IH��GPIEZIH�ERH�WITEVEXIH��8LI�YRGYX�fragment is 632 bp; cut fragments are 415 bp and 217 bp.

Scoring and Output'VIEXI��WEZI�ERH�MQTSVX�¾EK�GVMXIVME�SV�WQIEV�EREP]WMW�GVMXIVME� for scoring any data set. Results are displayed immediately in the Flag Analysis or Smear Analysis Table. Results can be exported directly from the Table by mouse functions or exported into either an Excel® or PDF reporting formats.

Page 3: Once in a while, a new productsuperior instrument, superior results 8li *vekqirx %rep]^iv %yxsqexih ') 7]wxiq lew fiir hiwmkrih jsv ¾i\mfpi stivexmsr tvszmhmrk lmkl witevexmsr viwspyxmsr

SUPERIOR INSTRUMENT, SUPERIOR RESULTS8LI�*VEKQIRX�%REP]^IV��%YXSQEXIH�')�7]WXIQ�LEW�FIIR�HIWMKRIH�JSV�¾I\MFPI�STIVEXMSR��TVSZMHMRK�LMKL�WITEVEXMSR�VIWSPYXMSR� over a wide range of DNA/RNA sizes and concentrations. Different gel kits are interchangeable with the same capillary array.

Instrument CapabilitiesFragment Analyzer™ Automated CE System Technical Specifications

Typical Separation — Speed and Resolution

Fast Run Times — Separate Fragments In Under 15 Minutes

(IXIGX�776W�;MXL�'SR½HIRGI�°�(S[R�XS���FT�6IWSPYXMSR

Separate and Quantify Genomic DNA

Automate Mutation Detection Analysis Detect induced (and natural) point mutations in genomic DNA. High detection sensitivity (as low as 5 pg/µL) combined with E�WMQTPM½IH�GPIEZEKI�TVSGIWW��RS�HIWEPXMRK�WXIT�SV�)(8%� EHHMXMSR �VIWYPX�MR�XLI�IJ½GMIRX�HIXIGXMSR�SJ�QYXEXMSRW��8LI�streamlined cleavage process and fast separation times yield improved throughput over manual TILLiNG methods.

Analyze Total RNA QualityNo exchange of capillary array is required, streamlining operation and reducing costs compared to alternative methods

Other Applications

DNA and RNA Quantity and Quality Measurements

Restriction Digestion of DNA Fragments (CAPS Analysis)

NGS Fragment Library Preparation

Mixture of GeneRuler™100 bp Plus Ladder (Fermentas) plus 35 bp (lower) and 6,000 bp (upper) marker fragments

Fast Separations: Parent 1, Parent 2 and Hetero SSR samples. The DNA fragments sized as 313 bp and 423 bp corresponding to < 10% sizing error. Outer peaks represent 200 bp/700 bp markers. Migration time for 700 bp upper marker was 13 min.

DNF-900 Gel: Separation demonstrating resolution of 113 bp, 115 bp, and122 bp DNA fragments.

Genomic DNA Kit: Separation of Ѥ DNA/HindIII Marker (Promega®) with intact (D1) and degraded (B1) genomic DNA.

ADVANCED ANALYTICAL > WWW.AATI-US.COM Fragment Analyzer™ AUTOMATED CE SYSTEM ADVANCED ANALYTICAL > WWW.AATI-US.COM Fragment Analyzer™ AUTOMATED CE SYSTEM

Fragment Analyzer™ Analytical Sofware

IMPROVE THROUGHPUT BY DECREASING ANALYSIS TIME PROSize™ data analysis software is designed to simplify JVEKQIRX�MHIRXM½GEXMSR�ERH�EYXSQEXI�VSYXMRI�WGSVMRK�ERHreporting of fragments.

Flexible Data DisplayData can be displayed in electropherogram or digital gel views for any combination of rows, columns or individual samples. Overlay random wells and adjust contrast, show/hide markers, zoom/autoscale and copy to other applications.

���SV����'ETMPPEV]�%VVE]�9WEKI – Direct, parallel injectionand separation of 12 or 96 samples at once.

Short Run Times – As short as 15 min for <3,000 bp. Fast separation of 12 samples or an entire 96-well plate cycle. Get more done in less time.

High Sensitivity – Detection limits as low as 5 pg/µL without desalting, allows direct injection of PCR and digest samples.

High Separation Resolution – Resolve a wide range of DNA fragments and RNA. Achieve as low as 2 bp resolution.Custom gels can be developed.

Wide Dynamic Range – Covers 3 orders of magnitude. 0IWW�STXMQM^EXMSR�RIIHIH��IEGL�OMX�KIP�LEW�E�HI½RIH�STXMQYQ� fragment concentration range.

Low Cost per Sample – Lower operational cost compared to agarose/acrylamide gels and other analytical instruments.

User-Friendly Software – Automate data scoring. Calculate fragment size and concentration, display data in multiple formats, create and save customized scoring parameters, export and report results with ease.

Real Time Viewing of Data – See results as they are being generated, change conditions during a run if desired.

Fragment Analyzer™ Key Features and Benefits

4SSPIH�(2%��IUYMZEPIRX�SJ���HMTPSMH�SVKERMWQW ��EQTPM½IH��GPIEZIH�ERH�WITEVEXIH��8LI�YRGYX�fragment is 632 bp; cut fragments are 415 bp and 217 bp.

Scoring and Output'VIEXI��WEZI�ERH�MQTSVX�¾EK�GVMXIVME�SV�WQIEV�EREP]WMW�GVMXIVME� for scoring any data set. Results are displayed immediately in the Flag Analysis or Smear Analysis Table. Results can be exported directly from the Table by mouse functions or exported into either an Excel® or PDF reporting formats.

Page 4: Once in a while, a new productsuperior instrument, superior results 8li *vekqirx %rep]^iv %yxsqexih ') 7]wxiq lew fiir hiwmkrih jsv ¾i\mfpi stivexmsr tvszmhmrk lmkl witevexmsr viwspyxmsr

Maximum Sample Throughput: Array dependent, either 12 or 96 samples

Maximum Unattended Sample Capacity: Up to 288 samples; unlimited with robotic arm

Minimum Sample Volume: 20 µL of liquid for injection; as little as 1 µL of actual sample required

Gel Sizing Ranges: 35-500 bp; 35-1,500 bp; 75-15,000 bp; 75 -20,000 bp

Kits Available: NGS Fragment Analysis, Genomic DNA, Total RNA, mRNA and TILLiNG

Resolution: Gel dependent; to as low as 2 bp

Sizing Accuracy: Typically 5% or better

Detection Sensitivity: As low as 5 pg/µL; 100pg/µL smear

Light Source: 1,000 mW LED, 470 nm excitation wavelength

Detector: High sensitivity CCD; 500-600 nm emission wavelength

Software: Fragment Analyzer™ instrument control, PROSize™ data analysis

Data Export Format: Microsoft® Excel® spreadsheet, binary output option

Environmental Conditions: Indoor use, normal laboratory environment 20-23°C

Relative Humidity Range: < 80% (non-condensing)

Electrical: � ��������:%'��������,^�����%��%PXIVREXI�GSR½KYVEXMSRW�EZEMPEFPI

Instrument Dimensions: � *YPP]�GSR½KYVIH����GQ�,�\����GQ�;�\����GQ�(�����\����\����MR

Instrument Weight: 38.5 Kg (85 lbs)

Advanced Analytical Technologies, Inc.2711 South Loop Drive, Suite 4150Ames, IA 50010 USAPhone: +1-515-296-6600Fax: +1-515-294-7141E-mail: [email protected]

Advanced Analytical Technologies, GmbH.Im Neuenheimer Feld 583D-69120 HeidelbergPhone: ++49 6221 868058-20Fax: ++49 6221 868058-99E-mail: [email protected]

Copyright © 2010 Advanced Analytical Technologies, Inc. All Rights Reserved. Reproduction, adaptation or translation without prior written permission is prohibited, except as allowed under copyright laws. Fragment Analyzer™ Automated CE System is a trademark of Advanced Analytical Technologies, Inc.

Once in a while, a new product comes along that changes how people work.

Fragment Analyzer™ Automated CE System

Fragment Analyzer™ Automated CE System Technical Specifications


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Warkah LMKL - June 2013

Warkah LMKL - June 2013

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