JOURNAL OF CLINICAL MICROBIOLOGYVOLUME 2 * 1975

EDITORIAL BOARDALBERT BALOWS, Editor-in-Chief(1979)

Centerfor Disease Control,Atlanta, Ga.

LORRAINE FRIEDMAN, Editor (1979)Tulane University Medical School,

New Orleans, La.

NATHALIE J. SCHMIDT, Editor (1975)California Department ofHealth,

Berkeley, Calif.

HENRY D. ISENBERG, Editor(1979)Long IslandJewish-Hillside Medical Center,

New Hyde Park, N.Y.

JOHN A. WASHINGTON II, Editor(1978)Mayo Clinic

Rochester, Minn.

Daniel Amsterdam (1977)Libero Ajello (1976)Hugo L. David(1975)Walter R. Dowdle (1976)V. R. Dowell, Jr. ( 1975)Richard W. Emmons (1977)John C. Feeley (1977)James D. Fenters ( 1976)Sydney M. Finegold (1976)Maxwell Finland (1975)Tbomas L. Gavan (1977)Morris A. Gordon (1975)Dieter Growchel (1977)James L. Hardy (1975)Melvin T. Hatch (1975)Lillian V. Holdeman (1976)

S. S. Kalter (1975)Leo Kaufman (1977)George E. Kenny (1976)Morton Klein (1975)George P. Kubica (1976)Howard W. Larsh (1977)James D. MacLowry (1977)William J. Martin (1975)John M. Matsen (1977)Joseph L. Melnick (1976)William 1. Metzger (1977)Maurice A. Mufson (1977)Demosthenes Pappagianis ( 1977)Robert H. Purcell (1977)William E. Rawls(1977)

Jon E. Rosenblatt( 1977)Margarita Silva-Hutner (1977)James W. Smith (1977)Louis DS. Smith (1975)Peter B. Smith (1976)Alex C. Sonnenwirth (1977)Welton 1. Taylor (1977)John H. Thompson, Jr. (1977)Marvin Turck ( 1977)Alexander von Graevenitz (1977)Kenneth W. Walls (1977)Lewis W. Wannamaker (1975)Ronald A. Ward (1977)Robert E. Weaver (1975)Robert P. Williams (I1978)Marion E. Wilson( 1977)

Robert A. Day, Managing EditorGisella Pollock, Director ofEditorial Services

Leslie Frymire, Production Editor1913 1St., N. W., Washington, D.C. 20006

EX OFFICIOHelen R. Whiteley, President (1975-1976)

J. Mehsen Joseph, Secretary

The Journal of Clinical Microbiology, a publication of theAmerican Society for Microbiology, 1913 I St., N.W., Washing-ton, D.C. 20006, is devoted to the dissemination of new knowl-edge concerning the applied microbiological aspects of humanand animal infections and infestations, particularly regardingtheir etiologic agents, diagnosis, and epidemiology. Papersdealing with antibiotics and antimicrobial agents and chemo-therapy, with fundamental aspects of infection and immunity.and with food or dairy microbiology fall within the scope ofother ASM publications. The Journal is published monthly,

Harlyn 0. Halvorson, Vice-President (1975-1976)Brinton M. Miller, Treasurer

and the twelve numbers are divided into two volumes per year.The nonmember subscription price is $50 per year. The mem-ber subscription price is $12 per year. Single copies are $5.Correspondence relating to subscription, reprints, defectivecopies, availability of back issues, lost or late proofs, dispo-sition of submitted manuscripts. and general editorial mattersshould be directed to the ASM Publications Office. 1913 1 St..N.W., Washington, D.C. 20006 (area 202 833-9416).

Application to mail at second-class postage rates is pending at

Washington, D.C.Made in the United States of America.Copyright 0 1975, American Society for Microbiology.1975, American Society for Microbiology.All Rights Reserved.

Author IndexAllen, Richard, 524Anderson, Roger L., 486Anhalt, John P., 510

Bennett, John V., 486Berger, J., 541Bredt, W., 541

Carter, Philip L., 474Chow, Anthony W., 546Crandell, Robert A., 465

Dee, Thomas H., 482Deidrick, Velma R., 478Dowdle, W. R., 531

Edberg, Stephen C., 469

Flanigan, S. M., 559Flodstrom, Anders, 504

Gray, Ernest D., 528Greenwood, J. R., 559

Grossgebauer, K., 516Guze, Lticien B., 546

Hallander, Hans O., 504Hedlund, Kenneth, 524Holmberg, Kenneth, 504

Kerner, H., 516Kuwert, E., 516

Lam, W., 541

Mackel, Donald C., 486Maki, Dennis G., 486Martin, W. J., 559Melloh, Ann J., 465Mostow, S. R., 531

Novak, Marta, 469

Patten, Valerie, 546Pemberton, John R., 549Peters, J., 516

Pickett, M. J., 559Pohle, H. D., 516

Rhame, Frank S., 486Rubin, Sally Jo, 556Ryan, Terence J., 474

Schild, G. C., 531Singer, Jacques MI., 469Slater, Helen, 469Slechta, Thomas F., 528Sorlie, Pamela, 465Spicher, G., 516Sumaya, Ciro V., 520Symington, Dorothy A., 498

Tilton, Richard C., 556Toma, S., 478

von Riesen, V. Lyle, 552, 554

Wood, R. J., 531

Yu, Pauline K. W., 510

JOURNAL OF CLINICAL MICROBIOLOGY

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ACKNOWLEDGMENTThe following have served as ad hoc reviewers from 11 September 1974 to 14 October 1975 and

their help is greatly appreciated.

H. E. AdlerDonald G. AhearnWilliam L. AlbrittonAaron D. AlexanderStephen D. AllenJohn P. Anhalt

Jack BaldwinR. A. BankowskiSamuel BaronArthur L. BarryJohn E. BennettDon J. BrennerSonja M. Buckley

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Judith K. DomerRobert M. DoughertyGordon DreesmanH. L. DuPont

Earl A. EdwardsHenry T. EigelsbachBennett L. ElisbergH. C. EllinghausenWallace Epstein

Richard R. FacklamJ. J. Farmer IIIPeter J. FischingerRobert H. Fitzgerald, Jr.

Murray B. GardnerJames H. GillespieNorman L. GoodmanDavid S. GordonDonald P. Gustafson

Adeline J. HackettCarlyn Halde

Leanor D. HaleyM. Marsha HallBerge HamparW. Knox HarrellLeonard HayflickGeorge HealyG. Ann HebertGertrude HenleJohn E. HerrmannKenneth L. HerrmannWilliam R. HessJohn HierholzerF. Blaine HollingerIan H. HolmesJohn E. HotchinRudolph HughMilton Huppert

Duane M. Ilstrup

Norman F. Jacobs

William KaplanAlfred G. KarlsonDouglas S. KelloggEdwin D. KilbourneGeorge C. KleinGeorge S. KobayashiStephen J. KrausKwon-Chung, Kyung-Joo

E. Y. LasfarguesMary P. LechevalierFlorence S. LiefJohn ListonH. Craig LyerlaWilliam R. McCabeBetty McGrewRoger M. McKinneyHarold MarkowitzRichard MasonGordon MeiklejohnP. J. MiddletonJames N. MillerRadmila MirkovicArnold S. MontoGeorge K. MorrisC. Wayne Moss

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Leslie A. PageBarbara G. PainterDavid ParetskyJ. W. PayneBertie PittmanAlfred M. Prince

Lucy RasmussenGladys H. ReynoldsJohn L. RiggsJohn W. RipponRoy E. Ritts, Jr.John B. RobbinsGlenn D. RobertsE. Arthur RobertsonLeon RosenArthur F. RosenthalPhilip K. Russell

A. J. SbarraJulius SchachterWilliam F. SchererJack H. SchiebleJohn D. SchneidauNathan J. SchneiderJohn SeverH. Jean ShadomyJoel B. SheffieldVella P. SilcoxJames T. SinskiKendall 0. SmithThomas F. SmithS. S. SocranskyWalter E. StammJohn D. StoboStanley S. StoneM. Sam SudmanNadine SullivanCatherine Sulzer

Eng M. Tan

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Ross J. WoodRichard E. Wooley

R. YamamatoViola Mae YoungPauline K. W. Yu

Ronald J. ZabranskyDonald W. Ziegler

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Announcing the definitive reference bookin clinical microbiology...

NEW SECOND EDITION OF Manual ofCLINICAL MICROBIOLOGY

Edited by EDWIN H. LENNETTE, Viral and Rickettsial Disease Laborat-ory, California Department of Health, Berkeley; EARLE H.SPAULDING,De-partment of Microbiology and Immunology, Temple University HealthSciences Center, Philadelphia; and JOSEPH P.TRUANT, Advance Medi-cal Laboratories, Pontiac, Michigan.

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AUTHOR INDEX

VOLUME 2

Abe, Takemaru, 89Adock, G. B., 206Adess, Michael, 382Ahearn, D. G., 354Allen, Richard, 524Altemeier, William A., 337Amstein, Charles F., 46Anderson, Roger L., 486Anhalt, John P., 510Ash, Ronald J., 270Ashley, C. R., 327Atherton, John M., 318

Bagley, Lauren R., 121Balcerzak-Raczkowski, I. B.,305

Balows, Albert, 235Banffer, J. R. J., 361Bannister, E. R., 149Barnhart, E. R., 270Bauman, David S., 77Beheshti, Firooz, 345Bennett, John V., 486Berger, J., 541Blank, F., 115Blazevic, Donna J., 266Blouse, Louis E., 318Bor, Eva, 226Bowman, P. I., 354Bredt, W., 541Brenner, Don J., 235Burdash, N. M., 149Burger, Gary T., 121Butler, Diane A., 322

Caie, Ian S., 153Calisher, Charles H., 198Caprioli, T., 311Carter, Philip L., 474Caul, E. O., 327Champion, Margaret L., 268Chappell, W., Adrian, 287Chow, Anthony W., 546Cosgrove, Jeanne O., 139Crandell, Robert A., 465Cummins, C. S., 104

Darland, Gary, 391Dee, Thomas H., 482Deidrick, Velma R., 478Dietel, John W., 430Delanerolle, V., 21Dick, C. R., 403Dick, E. C., 403Dilworth, John A., 453Dowdle, W. R., 531Driesens, Robert J., 85Drucker, D. B., 162

Duncan, R. C., 149Dyar, C., 149

Eckert, Herbert L., 42Edberg, Stephen C., 469Ederer, Grace M., 266Ehret, Josephine M., 142Eickhoff, Theodore C., 142Ellen, R. P., 305Emmons, Richard W., 81Eng, Sam, 67Eskenazy, M., 368Evans, James B., 14Everett, E. Dale, 142

Falcao, D. P., 349Farmer, J. J., III, 349, 359, 397Farrow, Wendell M., 419Faur, Yvonne C., 178Feldman, Harry A., 136Fink, Jordan N., 55Fischer, John B., 111Flanigan, S. M., 559Flodstrom, Anders, 504Forghani, Bagher, 410Foy, Hjordis, 226Frisch-Niggemeyer, W., 377Friz, Mary, 218Furukawa, Toru, 332

Gamble, William C., 287Gavan, Thomas L., 322Glosser, James W., 218Goldberg, I. D., 387Gordon, M. A., 74Goto, Hitoshi, 89Gray, Ernest D., 528Greenwood, J. R., 559Groschel, Dieter, 96Grossgebauer, K., 516Group$, Vincent, 419Guze, Lucien B., 546Gwaltney, Jack M., Jr., 453

Hahon, Nicholas, 42Hall, Marsha, 18Hall, Marsha, 296Hallander, Hans O., 504Hammar, Sherrel, 226Hanker, Jacob S., 463Harding, Sally A., 222Harkness, John L., 296Harper, Gloria, 21Hart, E. J., 387Hartman, Paul A., 46Hatch, Milford H., 231Hauser, K. J., 1Hedlund, Kenneth, 524

Hendley, J. Owen, 453Hickman, Frances W., 349Hickman, Robert, 226Hill, Edward O., 337Hiscott, Thomas, 186Ho, Helen H., 183Holdeman, Lillian V., 272Holland, Joseph W., 337Holmberg, Kenneth, 504Hopfer, Roy L., 96, 115Hopkins, J. M., 206Hornberger, Elizabeth, 332Horstmeier, Carlyle, 18Hubbard, Mary E., 186Hulst, A. M., 361Huppert, M., 21

Ilstrup, Duane M., 296Inhorn, S. L., 403Isenberg, Henry D., 99, 139Ivanova, R., 368

Jacobs, Norman F., Jr., 292Jarnagin, Jerald L., 268Jimenez-Ulate, Franklin, 441Jones, Lois F., 397Jones, Wallis L., 218Kalinova, R., 368Kanamitsu, Masatsugu, 89Kane, Judith A., 35Kane, Julius, IIIKarakawa, Walter K., 35Kasatiya, S. S., 311Kenny, George, 276Kenyon, Richard H., 121Kenyon, Richard H., 300Kerner, H., 516Kertesz, Vera, 461Klein, Dolph, 425Kolonel, Laurence, 318Kouroupis, G. M., 8Krause, George W., 345Kurup, Viswanath P., 55Kuwert, E., 516

Lagios, Michael D., 62Lam, W., 541Land, G. A., 206Larson, D., 253Lawman, Michael J. P., 153Lee, S. M., 162Lee, Wie-Shing, 62Leers, W. D., 8Legakis, N. J., 373, 459Lennette, David A., 81Lennette, Edwin H., 81, 183,410

Leonards, Richard, 62

ii AUTHOR INDEX

Lewis, Vester J., 193Lo, James W., 129Lobregat, Corazon M., 322Lunceford, C. D., 456Lund, Marlys E., 266

McGlynn, Doris M., 186McGonagle, Lee Anne, 67Mackel, Donald C., 486Maker, Myron D., 70Maki, Dennis G., 486Malinin, Theodore I., 243Malone, Beatrice H., 272Maness, Kathryn S. C., 198Mirrett, Stanley, 243Martin, R. S., 438Martin, W. J., 559Matsen, John M., 425Mayyasi, S., 253Melloh, Ann J., 465Merz, William G., 222Minor, T. E., 403Mirrett, Stanley, 247, 266Mitzel, J. R., 126Mizrahi, A., 11Monroe, Patrick W., 382Montes de Oca, Hector, 243Morgan, Patrick M., 382Mostow, S. R., 531Munch, K., 255

Norden, Carl W., 136Novak, Marta, 469

Osterman, Joseph V., 430

Page, L. A., 165Painter, Barbara G., 99Papavassiliou, J., 373, 459Patten, Valerie, 546Patterson, William R., 130Pedersen, Carl E., Jr., 121Pedersen, Carl E., Jr., 300Pemberton, John R., 144, 147,549

Peters, J., 516Pickett, M. J., 559Plotkin, Stanley A., 332Pohle, H. D., 516Probert, M., 157

Raahave, Dennis, 277

Rabin, Albert N., 463Rabkin, Elizabeth, 35Raghoenath, S., 361Reddick, Anne, 72Reller, L. Barth, 247, 266Rhame, Frank S., 486Richmond, Shirley J., 327Robart, E. M., 438Roberts, Glenn D., 18, 261Robertson, P. W., 461Rogers, Faye, 218Rubin, Sally Jo, 556Russell, S. M., 157Ryan, Terence J., 474Rytel, Michael W., 173

Sakuma, Sadatoshi, 332Salkin, I. F., 74Sammons, Lenora S., 121Sammons, Lenora St. C., 300Sande, Merle A., 453Scales, Roy W., 292Schelhart, Dorothy, 281Scherer, Jane S., 139Schild, G. C., 531Shimizu, Kiheiji, 89Schmidt, Gertrud, 67Schmidt, Nathalie J., 183, 410Schmitt, Martin W., 419Schneider, Nathan, J., 272Schreider, Minnie, 272Shayegani, Mehdi, 186Shepard, M. C., 456Shirai, Akira, 430Silberg, Stanley L., 382Simao, Emilia, 397Singer, Jacques M., 469Skaggs, Romulus, 292Slater, E. A., 126Slater, Helen, 469Slechta, Thomas F., 528Smith, Andrew G., 345Smith, Coy D., 77Smith, Kendall 0., 136Smith, Thomas F., 134Sol-Vernin, Carlos, 397Sorlie, Pamela, 465Sottile, Margaret I., 173Spicher, G., 516Spindler, Joyce A., 425Stalheim, 0. H. V., 165, 169Stauffer, Larry R., 337

Stephens, R., 253Stewart, John, 42Stewart, Paula, 453Strahilov, D., 368Strating, A., 126Stone, S. S., 169Sullivan, James D., Jr., 151Sulzer, Catherine R., 218Sumarah, R. K., 438Sumaya, Ciro V., 520Sun, Sung H., 21Symington, Dorothy A., 498

Taylor, Welton I., 281Tess, Bernard R., 129Thacker, W. Lanier, 193Thoen, Charles 0., 268Thomas, Evan T., 397Tilton, Richard C., 5,56Toma, S., 478Trabulsi, L. R., 349Traul, K., 253

Van Scoy, Robert E., 94Vinton, E. C., 206von Riesen, V. Lyle, 552, 554

Walter, Paul D., 193Washington, John A., II, 18, 70,

94, 296Watkins, Charles A., 318Watson, Stanley W., 151Weed, Lyle A., 134Weisburd, Martin H., 178West, M. E., 149White, Lendell A., 287Wilkins, Tracy D., 441Wilson, Marion E., 178Wilson, Walter R., 94Winkler, W. Gerald, 193Wirsing, Karla, 67Wolff, J., 253Wood, R. J., 531

Xilinas, M. E., 459

Yeager, Anne S., 448Yewdall, Russell C., 186Yu, Pauline K. W., 510

Zabranksy, R. J., 1Zaccour, F., 311

J. CLIN. MICROBIOL.

SUBJECT INDEX

VOLUME 2

Acetoin, 162Actinomyces nae8lundii, 305Actinomyces ViSCOsu8s, 305Agarose beads, 292Anaerobic bacteriaevaluation of new column packing material, 1modification of gas-liquid procedure, 1

Anaerobic growth of staphylococci, 14Anaerobic specimen transport device, 441Anaerobic strains of C. vaginale (H. vaginalis), 272Antibiotic susceptibility, 391Antibodyantinuclear determinations, 42influenza virus complement-fixing, 287B. canis, 382

Antigensformalinized bacterial, 359hepatitis B, 8, 231pneumococcal types VII and XIV, 510

Antinuclear antibody determinations, 42API 20E system, 322Arthritis

identification of M. agalactiae subsp. bovis from,169

of cattle, 165Aspergillus differential medium, 74A8pergillus fumigatus, 482Assay, plaque, 126Auxanographic method, 206

Bacilli, 149Bacteremia

detection of in culture bottles, 296incidence of in adults, 94

Bacteriaanaerobic, 1classification by direct inoculation procedure,469

recovery from peritonsillar abscesses, 504Bacterial identification, 391Bacterial phospholipases, 373Bactericidal test, 136Bacteriological sampling, 277Bacteroides, 337Batch adsorption, 528Blood culture bottles, 296Blood culture tube, 99Bovine group B streptococci, 35Bovine rhinotracheitis virus, 46.5Brucella canis antibodies, 382

Candida albicans, 345Carbohydrate fermentation test, 12Catalase-positive microorganisms, 463Chiamydia, 134Chlamydia trachomatis, 327Chlamydospore, 345Choriomeningitis virus infection, 193

iii

Chromatography, gas-liquid, 1, 162Classification of bacteria, 469Clinical isolation of Y. enterocolitica, 559Color reach on streak test, 463Complement fixation test, 77Congenital infection, 461Conjugates, 296Contamination of intravenous products, 486Corynebacterium vaginale, 272Counterimmunoelectrophoresiscomparison with Reiter protein, 361for subtyping hepatitis B antigen, 231of pneumococcal antigens, 510to detect A. fumigatus, 482to identify S. pneumoniae, 173

Coxsackieviruses (group A), 183Cryptococcus neoformans

identification of, 115identified by six-hour pigmentation test, 96

Culturesblood, bottle, 296blood, tube, 99human lymphocyte cell line, 11

Cytomegalovirus infections, 448

Dental plaque bacteria, 305Diacetyl, 162Differential medium, 305Dual-staining method, 149

Encise II, 139Enteric Analyzer

to identify Enterobacteriaceae, 186, 235Enteric pathogens, 281Enterobacteriaceae

identification by Enteric Analyzer, 186, 235identification of enteric pathogens, 247lactose-positive S. typhimurium, 349resembling E. coli, 70

Enterotube, 139Epidemiologic study, 520Equine influenza, 89Escherichia coli, 70

Fifty-milliliter vacutainer blood culture tube, 99Formaldehyde

retention of preservative levels, 144vapor, 516

Formalinized bacterial antigens, 359Four culture media, 438Fungi, 261Fusobacterium, 337

Gas-liquid chromatographyanalysis of acetoin and diacetyl, 162to identify anaerobic bacteria, 1

Genito-urinary tract, 178Glow discharge, 46

iv SUBJECT INDEX

Haemophilus influenzae, 136Hemagglutinationcompared with radioimmunoassay method, 8reversed passive, 8rubella virus, 524

Heparin, 151Hepatitis B antigenscomparison of methods for, 8counterelectrophoresis method, 231

Herpes simplex viral antibodies, 410Herpes simplex virus, 270Histoplasmosis, 77Human cytomegalovirus, 332Hypersensitivity pneumonitis, 55

Immunodiffusion test, 77Immunoelectrophoretic patterns, 129Immunofluorescence method, 81Immunofluorescence of yeast in urine, 142Immune fluorescence microscopy, 121Immunoglobulin coupled to agarose beads, 292Immunoglobulin G antibodies, 377Immunoglobulin M antibodies, 377Immunoglobulin G receptors, 332Indirect antibody technique, 153Indirect fluorescent antibody procedure, 337Indirect hemagglutination test

for diagnosis of human leptospirosis, 218for human antibody to typhus and rickettsiae,430

Indirect immunoferritin technique, 327Indole production, 425Infectionchoriomeningitis virus, 193cytomegalovirus, 448mumps virus, 81wound, 62, 556

Influenza A vaccine, 403Influenza type A virus, 531Influenza virus complement-fixing antibody, 287Intravenous products, 486

Klebsiella bacillus, 425

Japanese quail, 419

Leptospiral microscopy agglutination test, 474Leptospirosis, 218Less commonly used media, 554Leukocyte-transforming agent, 520Limulus test, 151Lymphocytic choriomeningitis virus infection, 193

Media table, 67Medium

Aspergillus differential, 74caffeic acid-containing, 115differential, 305motility-indole-lysine, 247motility-whole-lysine sulfide, 266

Mercurial preservatives in desiccated biologicalproducts, 549

Methodauxanographic, 206

counterelectrophoresis, 231dual-staining, 149hemagglutination, 8immunofluorescence, 81microscopy, 541serum-soft agar, 35

Microscopyimmune fluorescence, 121method to identify M. pneumoniac, 541phase-contrast, 261

Microtechnique, 474Modified fluorescent antibody technique, 461Motility-indole-lysine medium, 247Motility-indole-lysine-sulfide medium, 266Mouse ascitic fluid, 287Mumps virus infections, 81Mycobacterium paratuberculosis, 268Mycobacterium tuberculosis, 438Mycoplasma agalactiae subsp. bovis, 169Mycoplasma hominis, 226Mycoplasma pneumoniae, 541Mycoplasmal arthritis, 165

Neisseria, 72Neisseria gonorrhoeae

conditions required for colony-type stability of,387

improved transport system for, 498Neisseria meningitidis, 178

Oncornaviruses, 253

Parainfluenza-3 virus antibody, 157Peritonsillar abscesses, 504Phage typing scheme

for group D streptococci, 311S. epidermidis strains, 318

Phase-contrast microscopy, 261Phenol, 147Phospholipases, 373Photostatic copying of immunoelectrophoretic

patterns, 129Plaque assay of rabies virus, 126Pneumococcal antigens, 510Pneumococcimethods to improve detection of, 448, 453

Polyols, 11Polypectate digestion by Yersinia, 552Porcine kidney cell monolayers, 126Precipitin tests, 104Preparation of media, 554Procedure of gas-liquid chromatography, 1Propionibacterium acnes, 104Proteus, 459Prototheca wickerhamii, 62Pseudomonas aeruginosa, 397Pyruvate, 14

Quail cell cultures, 419

Radioimmunoassaycompared with hemagglutination method, 8for measurement of viral antibody, 130to determine antibody frequencies, 136

J. CLIN. MICROBIOL.

SUBJECT INDEX v

RD cells, 183Reiter protein, 361Renal cells, 243Respiratory secretions, 453Rickettsiae, 430Rickettsia rickettsii, 121Rocky Mountain spotted fever vaccines, 300Rubella infections, 377Rubella virus hemagglutination, 524

Saprophytic alga, 62Salmonella antisera, 368Salmonella lipopolysaccharides, 368Salmonella typhimuriumendemic in Brazil, 349unusual Enterobacteriaceae, 349

Seroagglutination test, 268Seroepidemiological investigation

of B. canis antibodies, 382on equine influenza, 89

Serum-soft agar method, 35Single radial hemolysis technique, 157Single radial diffusion test, 531Six-hour pigmentation test, 96Smallpox, 516Solid phase radioimmunoassay, 410Staphylococci, 14Staphylococcus epidermidis, 318Streptococcal nuclease B, 528Streptococcibovine group B, 35isolated from human urogenital tract, 311phage typing scheme, 311

Streptococcus pneumoniae, 173Syphilis, 361

Techniquecounterimmunoelectrophoresis, 173, 231, 361,

482, 510indirect antibody, 153indirect immunoferritin, 327single radial hemolysis, 157thin-layer chromatographic, 373

Temperature effect on media for enteric patho-gens, 281

Testbactericidal, 136carbohydrate fermentation, 72color reaction streak test, 463complement fixation, 77immunodiffusion, 77indirect hemagglutination, 218leptospiral microscopy agglutination, 474precipitin, 104radioimmunoassay, 136

seroagglutination, 268single radial diffusion, 531six-hour pigmentation, 96

Thermophilic actinomycetes associated with hy-persensity pneumonitis, 55

Thin-layer chromatographic technique, 373Tissue culture, 46Transport system for N. gonorrhoeae, 498Trichophyton megninii, 111Toxoplasma gondii, 461Typhoid vaccine, 85Typhus, 430

Ultraviolet fluorescence, 546Uni-Yeast-Tek kit, 354Uracil, 14Ureaplasma urealyticumprevalence in adolescents, 226unusual colonies of, 456

Urinary tract infection, 222Urogenital tract, 311

VaccineAlice, 403Rocky Mountain spotted fever, 300typhoid, 85

Velvet pads, 277Veillonella, 546Venezuelan equine encephalitis virus, 198Vibrio alginolyticus, 556Viral antibody, 130Virusbovine rhinotracheitis, 465choriomeningitis, 193herpes simplex, 270influenza type A, 531mumps, 81rabies, 126rubella, 524Venezuelan equine encephalitis, 198

Wound infectionby P. wickerhamii, 62isolation of V. alginolyticus, 556

Yeastsevaluation of antibody coating, 222identification by Uni-Yeast-Tek kit, 354immunofluorescence of, 142rapid methods to identify, 21recovery from blood culture bottles, 18

Yersinia, 552Yersinia enterocolitica

clinical isolation of, 559isolation from swine, 478

VOL. 2, 1975

INDEX TO DATE OF ISSUE

Month Date of Issue Pages

July 5 August 1975 1-75August 2 September 1975 77-151September 30 September 1975 153-276October 30 October 1975 277-360November 3 December 1975 361-464December 31 December 1975 465-560

JOURNALOF

CLINICAL

MICROBIOLOGY

VOLUME 2

WASHINGTON, D.C. 20006

1975

JOURNAL OF CLINICAL MICROBIOLOGYVOLUME 2 *NUMBER 6* DECEMBER 1975

EDITORIAL BOARDALBERT BALOWS, Editor-in-Chief(1979)

Centerfor Disease Control,Atlanta, Ga.

LORRAINE FRIEDMAN, Editor (1979)Tulane University Medical School,

New Orleans, La.

NATHALIE J. SCHMIDT, Editor(1975)California Department ofHealth,

Berkeley, Calif.

HENRY D. ISENBERG, Editor(1979)Long Island Jewish-Hillside Medical Center,

New Hyde Park, N. Y.

JOHN A. WASHINGTON II, Editor(1978)Mayo Clinic

Rochester, Minn.

Daniel Amsterdam (1977)Libero Ajello (1976)Hugo L. David (1975)Walter R. Dowdle (1976)V. R. Dowell, Jr. (1975)Richard W. Emmons (1977)John C. Feeley (1977)James D. Fenters (1976)Sydney M. Finegold (1976)Maxwell Finland (1975)Thomas L. Gavan (1977)Morris A. Gordon (1975)Dieter Groschel (1977)James L. Hardy (1975)Melvin T. Hatch (1975)Lillian V. Holdeman (1976)

S. S. Kalter (1975)Leo Kaufman (1977)George E. Kenny (1976)Morton Klein (1975)George P. Kubica (1976)Howard W. Larsh (1977)James D. MacLowry (1977)William J. Martin (1975)John M. Matsen (1977)Joseph L. Melnick (1976)William I. Metzger (1977)Maurice A. Mufson (1977)Demosthenes Pappagianis (1977)Robert H. Purcell (1977)William E. Rawls (1977)

Jon E. Rosenblatt (1977)Margarita Silva-Hutner (1977)James W. Smith (1977)Louis DS. Smith (1975)Peter B. Smith (1976)Alex C. Sonnenwirth (1977)Welton 1. Taylor (1977)John H. Thompson, Jr. (1977)Marvin Turck (1977)Alexander von Graevenitz (1977)Kenneth W. Walls (1977)Lewis W. Wannamaker (1975)Ronald A. Ward (1977)Robert E. Weaver (1975)Robert P. Williams (1978)Marion E. Wilson (1977)

Robert A. Day, Managing EditorGisella Pollock, Director ofEditorial Services

Leslie Frymire, Production Editor1913 I St., N. W., Washington, D.C. 20006

EX OFFICIOHelen R. Whiteley, President (1975-1976)

J. Mehsen Joseph, Secretary

The Journal of Clinical Microbiology, a publication of theAmerican Society for Microbiology, 1913 I St., N.W., Washing-ton, D.C. 20006, is devoted to the dissemination of new knowl-edge concerning the applied microbiological aspects of humanand animal infections and infestations, particularly regardingtheir etiologic agents, diagnosis, and epidemiology. Papersdealing with antibiotics and antimicrobial agents and chemo-therapy, with fundamental aspects of infection and immunity,and with food or dairy microbiology fall within the scope ofother ASM publications. The Journal is published monthly,

Harlyn 0. Halvorson, Vice-President (1975-1976)Brinton M. Miller, Treasurer

and the twelve numbers are divided into two volumes per year.The nonmember subscription price is $50 per year. The mem-ber subscription price is $12 per year. Single copies are $5.Correspondence relating to subscription, reprints, defectivecopies, availability of back issues, lost or late proofs, dispo-sition of submitted manuscripts, and general editorial mattersshould be directed to the ASM Publications Office, 1913 I St.,N.W., Washington, D.C. 20006 (area 202 833-9416).

Application to mail at second-class postage rates is pending atWashington, D.C.Made in the United States of America.Copyright i 1975, American Society for Microbiology.1975, American Society for Microbiology.All Rights Reserved.

Volume 2 Contents for July Number 1Modification of the Gas-Liquid Chromatography Procedure and Evaluation of a

New Column Packing Material for the Identification of Anaerobic Bacteria.K. J. HAUSER* AND R. J. ZABRANSKY .1

Comparison of the Reversed Passive Hemagglutination with RadioimmunoassayMethods for Hepatitis B Antigen. W. D. LEERS* AND G. M. KOUROUPIS 8

Pluronic Polyols in Human Lymphocyte Cell Line Cultures. A. MIZRAHI .. 11Uracil and Pyruvate Requirements for Anaerobic Growth of Staphylococci. JAMES

B. EVANS.14

Recovery of Yeasts from Blood Culture Bottles. GLENN D. ROBERTS, CARLYLEHORSTMEIER, MARSHA HALL, AND JOHN A. WASHINGTON II* ..18

Rapid Methods for Identification of Yeasts. M. HUPPERT,* GLORIA HARPER, SUNGH. SUN, AND V. DELANEROLLE.21

Demonstration of the Capsular Antigens of Bovine Group B Streptococci by theSerum-Soft Agar Method. JUDITH A. KANE,* ELIZABETH RABKIN, AND WALTERW. KARAKAWA.35

Evaluation of Cellular Substrates for Antinuclear Antibody Determinations.NICHOLAS HAHON,* HERBERT L. ECKERT, AND JOHN STEWART.42

Adaptation of Plastic Surfaces for Tissue Culture by Glow Discharge. CHARLESF. AMSTEIN AND PAUL A. HARTMAN*.46

A Scheme for the Identification of Thermophilic Actinomycetes Associated withHypersensitivity Pneumonitis. VISWANATH P. KURUP* AND JORDAN N. FINK .. 55

Wound Infection by Prototheca wickerhamii, a Saprophytic Alga Pathogenic forMan. WIE-SHING LEE,* MICHAEL D. LAGIOS, AND RICHARD LEONARDS.62

A Space-Saving Media Table. LEE ANNE MCGONAGLE,* GERTRUD SCHMIDT, SAMENG, AND KARLA WIRSING.67

Unclassified, Lactose-Fermentating, Urease-Producing Member of the FamilyEnterobacteriaceae Resembling Escherichia coli. JOHN A. WASHINGTON II*AND MYRON D. MAKER.70

A Simple Carbohydrate Fermentation Test for Identification of the PathogenicNeisseria. ANNE REDDICK.72

Evaluation of Aspergillis Differential Medium. I. F. SALKIN* AND M. A. GORDON .. 74

Volume 2 Contents for August Number 2Comparison of Immunodiffusion and Complement Fixation Tests in the Diagnosis

of Histoplasmosis. DAVID S. BAUMAN* AND CoY D. SMITH.77Rapid Diagnosis of Mumps Virus Infections by Immunofluorescence Methods.

DAVID A. LENNETTE, RICHARD W. EMMONS, AND EDWIN H. LENNETTE*.81Effect of Glass on pH-Dependent Stability of Typhoid Vaccine. ROBERT J.DRIESENS.85

Sero-Epidemiological Study on Equine Influenza in Japan. HrrOSHI GOTO,*KIHEIJI SHIMIZU, TAKEMARU ABE, AND MASATSUGU KANAMITSU.89

Incidence of Bacteremia in Adults Without Infection. WALTER R. WILSON, * ROBERTE. VAN SCOY, AND JOHN A. WASHINGTONII.94

Six-Hour Pigmentation Test for the Identification of Cryptococcus neoformans.RoY L. HOPFER* AND DIETERGROSCHEL.96

Clinical Laboratory Evaluation of the Fifty-Milliliter Vacutainer Blood Culture*Asterisk refers to person to whom inquiries regarding the paper should be addressed.

ix

Tube. BARBARA G. PAINTER* AND HENRY D. ISENBERG.99Identification of Propionibacterium acnes and Related Organisms by Precipitin

Tests with Trichloroacetic Acid Extracts. C. S. CUMMINS.104Occurrence of Trichophyton megninii in Ontario. Identification with A Simple

Cultural Procedure. JULIUS KANE AND JOHN B. FISCHER* ..i..l....l....illCaffeic Acid-Containing Medium for Identification of Cryptococcus neoformans.

Roy L. HOPFER* AND F. BLANK.115Demonstration of Rickettsia rickettsii in the Rhesus Monkey by Immune Fluores-

cence Microscopy. CARL E. PEDERSEN, JR.,* LAUREN R. BAGLEY, RICHARD H.KENYON, LENORA S. SAMMONS, AND GARY T. BURGER.121

Plaque Assay of Rabies Virus on Porcine Kidney Cell Monolayers. A. STRATING,*J. R. MITZEL, AND E. A. SLATER.126

Photostatic Copying of Immunoelectrophoretic Patterns with the Use of ColorPrint Filters. JAMES W. Lo* AND BERNARD R. TESS.129

Improvements of a Radioimmunoassay for Measurement of Viral Antibody inHuman Sera. WILLIAM R. PATrERSON* AND KENDALL 0. SMITH.130

Comparison of Urethral Swabs, Urine, and Urinary Sediment for the Isolation ofChlamydia. THOMAS F. SMITH AND LYLE A. WEED.134

Haemophilus influenzae, Type b, Antibody Frequencies Determined with Bacteri-cidal and Radioimmunoassay Tests. CARL W. NORDEN* AND HARRY A.FELDMAN.136

Clinical Laboratory Evaluation of the Further Improved Enterotube and EnciseII. HENRY D. ISENBERG,* JANE S. SCHERER, AND JEANNE COSGROVE......... 139

Immunofluorescence of Yeast in Urine. E. DALE EVERETT,* THEODORE C. EICKHOFF,AND JOSEPHINE M. EHRET.142

Retention of Preservative Levels of Formaldehyde in Dessiccated BiologicalProducts. JOHN R. PEMBERTON.144

Retention of Preservative Levels of Phenol in Desiccated Biological Products.JOHN R. PEMBERTON.147

Evaluation of a Dual-Staining Method for Acid-Fast Bacilli. N. M. BURDASH,*M. E. WEST, E. R. BANNISTER, C. DYAR, AND R. C. DUNCAN.149

Inhibitory Effect of Heparin on the Limulus Test for Endotoxin. JAMES D. SULLI-VAN, JR.,* AND STANLEY W. WATSON.151

Volume2 Contents for September Number3Design and Construction of an Apparatus for the Growth of Micro Cell Cultures

on Standard Glass Microscope Slides and Its Application for Screening LargeNumbers of Sera by the Indirect Antibody Technique. MICHAEL J. P. LAW-MAN* AND IAN S. CAIE.153

Measurement of Parainfluenza-3 Virus Antibody by the Single Radial HemolysisTechnique. M. PROBERT* AND S. M. RUSSELL.157

Analysis of Acetoin and Diacetyl in Bacterial Culture Supernatants by Gas-Liq-uid Chromatography. S. M. LEE AND D. B. DRUCKER*.162

Naturally Occurring and Experimentally Induced Mycoplasmal Arthritis of Cattle.0. H. V. STALHEIM* AND L. A. PAGE.165

Isolation and Identification of Mycoplasma agalactiae subsp. bovis from ArthriticCattle in Iowa and Nebraska. 0. H. V. STALHEIM* AND S. S. STONE.169

x CONTENTS

Application of Counterimmunoelectrophoresis in the Identification of Streptococcuspneumoniae in Clinical Isolates. MARGARET I. SOTTILE* AND MICHAEL W.RYTEL.173

Isolation of Neisseria meningitidis from the Genito-Urinary Tract and Anal Canal.YVONNE C. FAUR,* MARTIN H. WEISBURD, AND MARION E. WILSON.178

Propagation and Isolation of Group A Coxsackieviruses in RD Cells. NATHALIE J.SCHMIDT, HELEN H. Ho, AND EDWIN H. LENNETTE*.183

Evaluation of the Enteric Analyzer for Identification of Enterobacteriaceae.MEHDI SHAYEGANI,* MARY E. HUBBARD, THOMAS HISCOTT, DORIS M. McGLYNN,AND RUSSELL C. YEWDALL.186

Comparison of Three Tests for Serological Diagnosis of Lymphocytic Choriomen-ingitis Virus Infection. VESTER J. LEWIS, * PAUL D. WALTER, W. LANIERTHACKER, AND W. GERALD WINKLER.193

Laboratory Studies of Venezuelan Equine Encephalitis Virus in Equines, Texas,1971. CHARLES H. CALISHER* AND KATHRYN S. C. MANESS.198

Improved Auxanographic Method for Yeast Assimilations: a Comparison withOther Approaches. G. A. LAND, * E. C. VINTON, G. B. ADCOCK, AND J. M.HOPKINS.206

Evaluation of an Indirect Hemagglutination Test for the Diagnosis of HumanLeptospirosis. CATHERINE R. SULZER,* JAMES W. GLOSSER, FAYE ROGERS,WALLIS L. JONES, AND MARY FRIZ.218

Evaluation of Antibody Coating of Yeasts in Urine as an Indicator of the Siteof Urinary Tract Infection. SALLY A. HARDING AND WILLIAM G. MERZ* .... 222

Prevalence of Mycoplasma hominis and Ureaplasma urealyticum (T Strains) inUrine of Adolescents. HJORDIS FOY,* GEORGE KENNY, EVA BOR, SHERRELHAMMAR, AND ROBERT HICKMAN.226

Modified Counterelectrophoresis Method for Subtyping Hepatitis B Antigen.MILFORD H. HATCH.231

Evaluation of the Enteric Analyzer, an Instrument to Aid in the Identification ofEnterobacteriaceae. DON J. BRENNER* AND ALBERT BALOWS.235

Dispersion and Cultivation of Renal Cells After Short-Term Storage of Kidneys.HEcrOR MONTES DE OCA AND THEODORE I. MALININ*.243

Motility-Indole-Lysine Medium for Presumptive Identification of Enteric Path-ogens of Enterobacteriaceae. L. BARTH RELLER* AND STANLEY MIRRETT .... 247

New Method for the Removal of Extraneous Proteins from Purified Oncornaviruses.K. TRAUL,* D. LARSON, R. STEPHENS, J. WOLFF, K. MUNCH, AND S. MAYYASI ... 253

Detection of Fungi in Clinical Specimens by Phase-Contrast Microscopy. GLENND.ROBERTS.261

Motility-Indole-Lysine-Sulfide Medium. GRACE M. EDERER,* MARLYS E. LUND,DONNA J. BLAZEVIC, L. BARTH RELLER, AND STANLEY MIRRETT.266

Seroagglutination Test for Identification of Mycobacterium paratuberculosis.JERALD L. JARNAGIN,* MARGARET L. CHAMPION, AND CHARLES 0. THOEN ...... 268

Optimal Cooling and Warming Rates in the Preservation of Herpes Simplex Virus(Type 2). RONALD J. ASH* AND E. R. BARNHART.270

Obligately Anaerobic Strains of Corynebacterium vaginale (Haemophilus vaginalis).BEATRICE H. MALONE, MINNIE SCHREIBER, NATHAN J. SCHNEIDER, AND LILLIAN V.HOLDEMAN.272

ERRATUMRapid Methods for Identification of Yeasts. H. HUPPERT,* GLORIA HARPER, SUNG

H. SUN, AND V. DELANEROLLE.276

XiCONTENTS

Volume2 Contents for October Number4New Technique for Quantitative Bacteriological Sampling of Wounds by Velvet

Pads: Clinical Sampling Trial. DENNIS RAAHAVE.277Effect of Temperature on Transport and Plating Media for Enteric Pathogens.

WELTON I. TAYLOR* AND DOROTHY SCHELHART.281Production of Influenza Virus Complement-Fixing Antibody in Mouse Ascetic

Fluid. LENDELL A. WHITE,* WILLIAM C. GAMBLE, AND W. ADRIAN CHAPPELL .. .. 287

Use of Immunoglobulin Coupled to Agarose Beads for Examining the Specificity ofConjugates. Roy W. SCALES,* NORMAN F. JACOBS, JR., AND ROMULUS SKAGGS .. 292

Effects of Atmosphere of Incubation and of Routine Subcultures on Detection ofBacteremia in Vacuum Blood Culture Bottles. JOHN L. HARKNESS, MARSHAHALL, DUANE M. ILSTRUP, AND JOHN A. WASHINGTON II*.296

Comparison of Three Rocky Mountain Spotted Fever Vaccines. RICHARD H.KENYON,* LENORA ST. C. SAMMONS, AND CARL E. PEDERSEN,JR. 300

Differential Medium for Detecting Dental Plaque Bacteria Resembling Acti-nomyces viscosus and Actinomyces naeslundii. R. P. ELLEN* AND I. B.BALCERZAK-RACZKOWSKI.305

Phage Typing Scheme for Group D Streptococci Isolated from Human UrogenitalTract. T. CAPRIOLI, F. ZACCOUR, AND S. S. KASATIYA*.311

Efficacy of Phage Typing Epidemiologically Related Staphylococcus epidermidisStrains. LOUIS E. BLOUSE,* LAURENCE N. KOLONEL, CHARLES A. WATKINS, ANDJOHN M. ATHERTON .. .. .. .. .. .. .. .. .. .. .. .. .. .. .. .. .. .. .. .. .;.. .. .. .. .. 318

Reproducibility of the Analytab (API 20E) System. DIANE A. BUTLER, CORAZONM. LOBREGAT, AND THOMAS L. GAVAN*.322

Identification of the Elementary Bodies of Chlamydia trachomatis in the ElectronMicroscope by an Indirect Immunoferritin Technique. C. R. ASHLEY,SHIRLEY J. RICHMOND, * AND E. 0. CAUL.327

Demonstration of Immunoglobulin G Receptors Induced by Human Cytomega-lovirus. TORU FURUKAWA,* ELIZABETH HORNBERGER, SADATOSHI SAKUMA, ANDSTANLEY A. PLOTKIN.332

Indirect Fluorescent Antibody Procedure for the Rapid Detection and Identifica-tion of Bacteroides and Fusobacterium in Clinical Specimens. LARRY R.STAUFFER,* EDWARD 0. HILL, JOSEPH W. HOLLAND, AND WILLIAM A. ALTEMEIER . 337

Germ Tube and Chlamydospore Formation by Candida albicans on a New Medium.FIROOz BEHESHTI, ANDREW G. SMITH,* AND GEORGE W. KRAUSE.345

Unusual Enterobacteriaceae: Lactose-Positive Salmonella typhimurium Which isEndemic in Sao Paulo, Brazil. D. P. FALCAO, L. R. TRABULSI, FRANCES W.HICKMAN, AND J. J. FARMER III* 349

Evaluation of the Uni-Yeast-Tek Kit for the Identification of Medically ImportantYeasts. P. I. BOWMAN AND D. G. AHEARN*.354

Formalinized Bacterial "Antigens" as a Potential Infection Hazard. J. J.FARMER III.359

Volume 2 Contents for November Number 5Comparison of the Counter-Immunoelectrophoresis Technique with the Reiter

Protein and Three Other Serological Tests as First Line Test for Syphilis.J. R. J. BXNFFER,* S. RAGHOENATH, AND A. M. HULST.361

xii CONTENTS

Insolubilized Salmonella Lipopolysaccharides as Efficient Immunoadsorbents forthe Preparation of Specific Salmonella Antisera. M. ESKENAZY,* D. STRAHILOV,R. IVANOVA, AND R.KALINOVA.368

Thin-Layer Chromatographic Technique for Rapid Detection of' Bacterial Phos-pholipases. N. J. LEGAKIS* AND J. PAPAVASSILIOU.373

Rapid Separation of Immunoglobulin M from Immunoglobulin G Antibodies for Re-liable Diagnosis of Recent Rubella Infections. W. FRISCH-NIGGEMEYER . 377

Seroepidemiological Investigation of Brucella canis Antibodies in Different HumanPopulation Groups. PATRICK W. MONROE,* STANLEY L. SILBERG, PATRICK M.MORGAN, AND MICHAELADESS.382

Conditions Required for the Attainment of Colony-Type Stability of' Neisseriagonorrhoeae in Liquid Culture. E. J. HART* AND I. D. GOLDBERG .387

Discriminant Analysis of Antibiotic Susceptibility as a Means of Bacterial Identifi-cation. GARYDARLAND.391

Epidemiology of Pseudomonas aeruginosa in a General Hospital: a Four-YearStudy. EVAN T. THOMAS,* Lois F. JONES, EMILIA SIMAO, CARLOS SOLE'-VERNIN,AND J. J. FARMERIII.397

Attenuated Influenza A Vaccine (Alice) in an Adult Population: Vaccine-RelatedIllness, Serum and Nasal Antibody Production, and Intrafamily Transmission.T. E. MINoR, E. C. DICK,* C. R. DICK, AND S. L.INHORN.403

Solid Phase Radioimmunoassay for Typing Herpes Simplex Viral Antibodies inHuman Sera. BAGHER FORGHANI, NATHALIE J. SCHMIDT, AND EDWIN H. LEN-NETTE .410

Responses of Isolator-Derived Japanese Quail and Quail Cell Cultures in SelectedAnimal Viruses. WENDELL M. FARROW,* MARTIN W. SCHMITr, AND VINCENTGROUP'E.419

Relationship of Indole Production and Antibiotic Susceptibility in the Kiebsiellabacillus. DOLPH KLEIN, JoycE A. SPINDLER, AND JOHN M. MATSEN* ......425

Indirect Hemagglutination Test for Human Antibody to Typhus and Spotted FeverGroup Rickettsiae. AKIRA SHIRAI, JOHN W. DIETEL, AND JOSEPH V. OSTERMAN* 430

Comparison of Four Culture Media for the Isolation of Mycobacterium tuberculosis:a 2-Year Study. R. S. MARTIN,* R. K. SUMARAH, AND E. M. ROBART .--------438

Anaerobic Specimen Transport Device. TRAcy D. WILKINS* AND FRANKLIN JIME-NEZ-ULATE.441

Longitudinal, Serological Study of Cytomegalovirus Infections in Nurses and inPersonnel Without Patient Contact. ANNE S. YEAGER.448

Methods to Improve Detection of Pneumococci in Respiratory Secretions. JOHNA. DILWORTH, PAULA STEWART, JACK M. GWALTNEY, JR., J. OWEN HENDLEY, AND45MERLE A.SANDE*45

Unusual Colonies of Ureaplasma urealyticum (T Mycoplasmas) in Primary AgarCultures of Certain Urine Sediments. M. C. SHEPARD AND C. D. LUNCE- 456FORD.

Selective Medium for Growth of Proteus. M. E. XILINAS,* J. T. PAPAVASSILIOU,AND N. H.LEGAKIS.459

Modified Fluorescent Antibody Technique to Detect Immunoglobulin M Antibodiesto Toxoplasma gondii in Congenital Infection. P. W. ROBERTSON* AND VERAKERTESZ.461

Color Reaction Streak Test for Catalase-Positive Microorganisms. JACOB S.HANKER* AND ALBERT N.RABIN.463

CONTENTS xiii

Volume 2 Contents for December Number6Sensitivity of Infectious Bovine Rhinotracheitis Virus to Ether. ROBERT A. CRAN-

DELL,* ANN J. MELLOH, AND PAMELA SORLIE.465

Direct Inoculation Procedure for the Rapid Classification of Bacteria from BloodCulture. STEPHEN C. EDBERG,* MARTA NOVAK, HELEN SLATER, AND JACQUESM. SINGER.469

New Microtechnique for the Leptospiral Microscopy Agglutination Test. PHILIPL. CARTER* AND TERENCE J. RYAN.474

Isolation of Yersinia enterocolitica from Swine. S. TOMA* AND VELMA R.DEIDRICK.478

Detection of Aspergillus fumigatus Serum Precipitins by Counterimmunoelec-trophoresis. THOMAS H. DEE.482

Nationwide Epidemic of Septicemia Caused by Contaminated Intravenous Prod-ucts: Mechanisms of Intrinsic Contamination. DONALD C. MACKEL, * DENNISG. MAKI, ROGER L. ANDERSON, FRANK S. RHAME, AND JOHN V. BENNETT .... ... 486

Improved Transport System for Neisseria gonorrhoeae in Clinical Specimens.DQOROTHY A. SYMINGTON.498

Influence of the Collection and Transport of Specimens on the Recovery ofBacteria from Peritonsillar Abscesses. HANS 0. HALLANDER,* ANDERS FLOD-STROM, AND KENNETH HOLMBERG.504

Counterimmunoelectrophoresis of Pneumococcal Antigens: Improved Sensitivityfor the Detection of Types VII and XIV. JOHN P. ANHALT AND PAULINE K.W. Yu.510

Experiments on Terminal Disinfection by Formaldehyde Vapor in the Case ofSmallpox. K GROSSGEBAUER,* G. SPICKER, J. PETERS, E. KUWERT, H. D.POHLE, AND H. KERNER.516

Epidemiologic Study of a Leukocyte-Transforming Agent in a General Population.CIRO V. SUMAYA.520

Simple Procedure for the Removal of Nonspecific Inhibitors of Rubella VirusHemagglutination. RICHARD ALLEN AND KENNETH HEDLUND*.524

Isolation of Streptococcal Nuclease B by Batch Adsorption. THOMAS F. SLECHTAAND ERNEST D. GRAY*.528

Application of the Single Radial Diffusion Test for Assay of Antibody to In-fluenza Type A Viruses. S. R. MOSTOW,* G. C. SCHILD, W. R. DOWDLE, ANDR. J. WOOD 531

Evaluation of a Microscopy Method for Rapid Detection and Identification ofMycoplasma pneumoniae. W. BREDT,* W. LAM, AND J. BERGER.541

Rapid Screening of Veillonella by Ultraviolet Fluorescence. ANTHONY W. CHOW,*VALERIE PATTEN, AND LUCIEN B. GUZE.546

Retention of Mercurial Preservatives in Desiccated Biological Products. JOHNR. PEMBERTON.549

Polypectate Digestion by Yersinia. V. LYLE VON RIESEN.552Convenient, Simplified Preparation of Less Commonly Used Media. V. LYLE

VON RIESEN.554Isolation of Vibrio alginolyticus from Wound Infections. SALLY Jo RUBIN*

AND RICHARD C. TILTON 556Clinical Isolation of Yersinia enterocolitica: Cold Temperature Enrichment. J. R.

GREENWOOD, S. M. FLANIGAN, M. J. PICKETT,* AND W. J. MARTIN.559

CONTENTSX1V