NIST Standard Reference Materials for Nucleic Acids - … Holden - SoGAT 2012... · NIST Standard...
Transcript of NIST Standard Reference Materials for Nucleic Acids - … Holden - SoGAT 2012... · NIST Standard...
NIST Standard Reference Materials for Nucleic Acids
Marcia Holden, Ross Haynes, Margaret Kline, John Butler
National Institute of Standards and Technology
Applied Genetics Group
Gaithersburg, Maryland, USA
SoGAT Blood Virology meeting Vilnius, Lithuania April 16 – 17 2012
NIST is a non-regulatory federal agency within the U.S. Department of Commerce. NIST’s primary mission is to promote economic growth by working with industry to develop and apply technology, measurements, and standards.
Measurement and Standards Program planned and conducted in cooperation with industry and focused on infrastructural technologies
NIST is the national measurement institute for the United States
Hollings Manufacturing Extension Partnership Program a nationwide network of extension centers that provides hands-on technical assistance to smaller manufacturers
Baldridge National Quality Program an outreach program recognizing organizational performance excellence
Applied Genetics Group Biochemical Science Division
Mission Statement
Advancing technology and traceability through quality genetic measurements and standard reference materials (SRMs) to aid work in
• Forensic DNA testing
• DNA biometrics
• Clinical diagnostics – Infectious disease
– genetic biomarkers
– cancer-linked biomarkers
NIST DNA Reference Materials (1)
Forensic Applications • STR PCR DNA Profiling (SRM 2391b)
• Human Y-Chromosome DNA Profiling (SRM 2395)
• Human DNA Quantitation (SRM 2372)
• Mitochondrial DNA Sequencing (SRM 2392-1
SRM 2392)
NIST DNA Reference Materials (2)
Clinical Diagnostics • Cytomegalovirus (SRM 2366)
• Fragile X Human DNA triplet repeat (SRM 2399)
• Huntington's Disease CAG Repeats (SRM 2393)
Platform Testing • Heteroplasmic mtDNA Mutation Detection (SRM
2394)
• DNA Sequence Library for External RNA Controls (SRM 2374)
Certified Reference Material (CRM)
• Reference material (RM), one or more of whose property values are certified by a procedure which establishes its traceability to an accurate realization of the unit in which the property values are expressed, and for which each certified value is accompanied by an uncertainty at a stated level of confidence and accompanied by a certificate
• Standard Reference Materials (SRMs) are CRMs certified by NIST
Traceability to the International System of Units (SI)
• We want to develop characterized, stable, homogeneous nucleic acid SRMs that are traceable to the SI unit, the mole (amount of substance)
• The approach to quantitative traceability is to count the number of molecules of a dilute solution of DNA in a known volume.
• Our goal is to achieve accurate counting that is assay independent
Why quantitative SRMs for clinical diagnostics
• To provide higher order standards for clinical diagnostics that can be used in the validation of a measurement system or to assign values to calibrants
Characterization - What and How
• DNA sequence of regions of the genome is verified using Sanger sequencing
• The copy number per volume is certified using digital PCR
Cytomegalovirus SRM 2366
• CMV material consists of the Towne Strain cloned into a bacterial artificial chromosome
• The BAC (Towne 147) was propagated, the DNA was purified and packaged in Teflon tubes
• Three different concentrations were prepared and tubes were randomly selected for quantification
Sequenced regions of the CMV genome
• Regions that were chosen to be sequenced were selected because they are targets for amplification assays, based on 65 published PCR assays and information from commercial assay manufacturers
• Sequencing was performed in both directions • The sequencing primers were designed to have significant overlap • The sequenced regions (total of 15,000 bp) was an exact match for
the Towne strain as deposited in GenBank with the exception of one base (UL54, base 78651)
Digital PCR (dPCR) for certification of SRM 2366
• dPCR is a process where individual molecules are counted using the fluorescent signal generated by a PCR reaction when amplification of DNA occurs
• This concept has been around since the 1999 but was only practical very recently with the development of microfluidic and droplet platforms where thousands of reactions are conducted simultaneously in chambers with nanoliter volumes/chamber
• No calibration curve is needed
X 1 2 3 4 5 6 7 8 9101112 X H H
Pressurized valve
Samples: 1-12
Water: H
Pressurized valve
dPCR microfluidics array
765 individual chambers / panel 12 panels/chip = 9180 chambers Each chamber – 6 nL
Concentration
• Saturated
≥ 1 copy in each well
• Binary detection
Calculate concentration
• No amplification
No target
Recommendations for the optimal range/765 chamber panel to minimize measurement uncertainty
Institution Positive wells Targets/panel Average Copies/well
Platform 200 to 700 232 to 1902 0.3 to 2.5
manufacturer
National Cancer 300 to 600 382 to 1178 0.5 to 1.5
Institute USA
Published* 409 to 734 585 to 2453 0.8 to 3.2
* Bhat S. et al. 2009 Anal. Bioanal. Chem. 394:457-467
dPCR
Determines absolute concentration of DNA
through the use of counting and statistics 550 wells with target (+)
215 wells without target (-)
550 positive wells statistics 212 copies/ L
765 wells / sample
Poisson Distribution
• Probability statistics of counting rare events
• Rare not every partition (time or space) has event (in this case, DNA)
• dPCR If you know how many wells have target you can estimate the total number of targets
0%
10%
20%
30%
40%
50%
60%
70%
0 1 2 3 4 5 6 7 8 9 10
We
lls
(%
)
molecules/well
5,000 molecules
10,000 molecules
30,000 molecules
Poisson Distribution
Thousands of molecules over 10,000 wells
Positive wells Negative wells
More conc. means more likely to have well with >1 molecule/well Change in % negative wells with
concentration 10,000 molecules 25% of wells with >1 copy
30,000 molecules 80% of wells with >1 copy
Quantification of SRM 2366 components
• Each component was separately quantified using multiple digital arrays
• Utilized separate assays targeting different regions of the genome, and found that the results were not significantly different – no bias
• One assay was used for the rest of the measurements
Commutability
• Component B of SRM 2366 was included in the Quality Control for Molecular Diagnostics (QCMD) 2010 CMV EQA program
• Participants were asked to add the DNA directly to the assay rather than extracting the DNA along with the QCMD test samples
• 178 participants submitted data. Laboratory developed assays were used to generate 78 datasets and commercial assays were used for 100 datasets
• The median consensus value was 5.9 log10 (Component B = 6.2 log10 based on dPCR)
Commutability
0.0
0.1
0.2
0.3
0.4
0.5
0.6
0.7
0.8
1 2 3 4 5 6 7 8 9
Log10(Copies per mL)
Centered Histogram
Kernal Density
Gaussian Model
FWHM
Re
lative
Are
a, #
Bin
/ (
#T
ota
l Δ
x)
QCMD CMV EQA - Participants & assays # Data sets Mean SD Median MADe
Total Datasets 178 5.845 0.674 5.900 0.486
Conventional Commercial 5 5.670 0.672 5.854 0.872
Real-Time Laboratory developed - Total 78 5.909 0.756 6.002 0.650
Real-Time Commercial - Total 95 5.795 0.600 5.826 0.451
Commercial kit A (1) 6 5.859 0.386 5.864 0.150
Commercial kit A (2) 15 6.224 0.345 6.205 0.332
Commercial kit B 21 5.632 0.880 5.733 0.794
Commercial kit C 28 5.767 0.321 5.821 0.326
Commercial kit D 12 5.789 0.233 5.776 0.298
dPCR
• Digital PCR has the possibility to be a realized reference method for quantification of nucleic acids
• NIST is working with other national measurement institutions such as IRMM, LGC, NMI Australia and KRISS in collaboration with each other and through BIPM (CCQM) to validate digital PCR
• NIST is working on other candidate SRMs and will be using dPCR for quantification
Thank you for the opportunity to make a presentation at SoGAT
Clinical Diagnostics Team Ross Haynes
Marcia Holden
Margaret Kline
John Butler, Group Leader