Nic mab3 project ås 16.11.12
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Transcript of Nic mab3 project ås 16.11.12
The MacroAlgaeBiorefinery -
sustainable
production of bioenergy carriers and high
value aquatic fish feed from macroalgae
- the MAB3 project
Anne-Belinda Bjerre, Teknologisk Institut, DenmarkLars Nikolaisen, Teknologisk Institut, Denmark16th November 2012. Workshop in Nordic Algae Network and Blue Bio, Ås, Norway
Algae
More than 1500 species in the Danish sea waters all with different chemical compositions.
More than 1500 species in the Danish sea waters all with different chemical compositions.
Title: Sustainable production of 3G energy carriers (ethanol, butanol og biogas) and fish feed from macroalgae (Laminaria digitata and Saccharina latissima)
Project period: 1st of March 2012 - 1st of March 2016
Financied by the Danish Strategic Research Council (20,4 mill. DKK total budget på 24 mill. DKK)
12 Partnere from Denmark, Irland, Italy, Germany
Education of 4 ph.d. and 2 post docs
Coordinator: Danish Technological Institute v/ Anne-Belinda Bjerre
The MacroAlgaeBiorefinery : MAB3
Partners
Danish Technological Institute (Coordinator) Aarhus University (AaU) (2 institutes) Technical University of Denmark (DTU) (2 institutes) National University of Ireland, Galway University of Hamburg University of Siena Danish Shellfish Centre Orbicon A/S DONG Energy A/S Aller Aqua A/S Vitalys I/S Dangrønt Products A/S
Novozymes participates as affiliated partner (delivery of enzymes and participating in the advisory board)
Biorefinery
Definition:
Integrated and combined processes for the conversion of biomass into a variety of food, feed, chemicals, biomaterials, and energy – at the same time maximising the value of the biomass and minimising the waste
Transportation fuel from algae
Microalgae: High contents of lipids (25-35%)
Macroalgae: High contents of carbohydrates (45-65%)
History of bio-ethanol (fuel ethanol) production in USA , the driver in biorefinery development
Ethanol Production in US
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In MAB3, fish feed (protein) will be the value added product, derived from production of energy carriers e.g. ethanol or biogas
In MAB3, fish feed (protein) will be the value added product, derived from production of energy carriers e.g. ethanol or biogas
Introduction to MAB3
Hypothesis: 1) Two brown macroalgae can be upgraded to energy carriers (either bioethanol, buthanol or biogas or combinations hereof) by conversion of 80% of the fermentable sugars, leaving behind a concentrated solid fraction rich in protein and (for liquid biofuels also) lipids, which can be used for fish feed.
2) A substantial amount (90%) of the remaining, undigested sugars i.e. the C5 sugars can be converted to additional value-added amino acids (isoleucine and arginine) for fish feed supplement.
Best practical methods, processes and technologies will be tested and optimised to meet these goals Best practical methods, processes and technologies will be tested and optimised to meet these goals
The project
MAB3: Financed by the Danish Strategic Research Counsil
WP1: Cultivation and harvesting
WP2: Pretreatment and storage
WP3: Liquid biofuels.Ethanol and butanol
WP4: Gaseous biofueland amino acids
WP5: Fish feed
WP7: DisseminationWP6: Sustainability and feasibility
WP8: Management
How brown algae are composed Brown Algae lack real, distinct, secondary cell walls (no lignin).
The cell walls in brown algae thalli are made up mainly of cellulose “micro-
fibrils” or fibrils forming a felty network.
The fibrils are rarely ordered in parallel manner as in higher plants or even
some green algal species.
In brown algae, these felty fibre networks are layered and embedded in a
polysaccharide matrix.
Gentle pretreatment technologies will be needed for disrupting the biomass before enzyme hydrolysis
Production of ethanol (or butanol) and protein from algae biomass
Ethanol (l)
Protein (s)
Ethanol fermenting strains
Auger pressing of Laminaria digitata from August harvest 2012
Ethanol production from Chaetomorpha linum testing different pretreatment conditions
Schultz-Jensen et al 2012, in preparation
Ball milling most efficient
pretreatment method for
ethanol production
(19 g/100g)
Ball milling most efficient
pretreatment method for
ethanol production
(19 g/100g)
Conclusions:
Brown algae are fine substrates for ethanol production due to high contents of polysaccharides. Challenges are:
– Identification of most suitable enzyme mixtures for fully hydrolysis to monomeric sugars
Washing and auger pressing were efficient pretreatment methods for water and salt removal in green algae, new test on brown algae have been performed.
– Room for improvement e.g. by enzyme treatment.
Brown algae lack real, distinct, secondary cell walls (no lignin). – Pretreatment conditions (prior to enzymatic hydrolysis) are less severe
than for lignocellulosic biomass materials:
Ball milling was the most effective pretretment of Chatamorpha linum prior to SSF with Baker’s yeast fermentation in combination with Celluclast and Novozym 188.
– Improved yields are expected using more targeting enzymes and other microorganisms during fermentation.
Acknowledgements
Danish Strategic Research Council, Programkomiteen for Bæredygtig Energi og Miljø, for financial support
Project partners for co-financing the project Novozymes for delivery of enzymes and chairing the
advisory board
Thank you for your attention
Web-site about MAB3 www.mab3.dk
Contacts about MAB3: Anne-Belinda Bjerre: [email protected]
Karin Svane Bech: [email protected] Nikolaisen: [email protected]
Annette Bruhn: [email protected]