Biogas Production - SINTEF · PDF fileBiogas Production – Microbial Teamwork and...

Biogas Production – Microbial Teamwork and Enzymology

CenBio Final Conference Ås Campus 13-14 March 2017

Live H. HagenKBM, Norwegian University of Life Sciences (NMBU), Ås, Norway Contact: [email protected]

Overview Background on biogas production with focus on the microbial teamwork behind

Project objectives

How we explore the microbial world

What we found - and what remains unknown

Current status and Future perspective

Organic waste management

Microbial digestion under anaerobic condition

Production of biogas (CH4, CO2)

Electricity – Heath – Biofuel

Norwegian University of Life Sciences 4

Biogas production

Organic waste management

Microbial digestion under anaerobic condition

Production of biogas (CH4, CO2)

Electricity – Heath – Biofuel


Biogas production

Enzymology During anaerobic digestion, microbes produce different enzymes that catalyzes different degradation events

Enzymology is the study of enzymes, their kinetics, structure, and function, as well as their relation to each other

Hydrolysis – substrate-composition dependent

Reconstruction of metabolic pathways

Norwegian University of Life SciencesTittel på presentasjon 6

Goals:Identify key organisms and metabolic pathways in biogas production

–Enhanced understanding of the process–More stable biogas reactors–Improved biogas production–Monitor and control the microbial process


MethodsTraditional Cultivation High-Throughput Sequencing


• In depth studies of specific organisms

• Compositions and Interactions

• Metagenomics• Closer to reality

MetagenomicsA Metagenome is all the genetic material present in an environmental sample, consisting of the genomes of many individual organisms

Metagenomics is the direct genetic analysis of metagenomes



Direct Sequencing of the Metagenome

• Identified microorganisms related to unstable performance

• Tested the tolerance for storage

• Studied the effect of recyclingthe effluent to dilute food waste

• Observed an improved process when adding cow manure


Experiments with Laboratory Biogas Reactors

Biogas lab facility at Vollebekk, Ås


Plant Scale Thermophilic Reactor FREVAR FK (Fredrikstad):• Receives food waste and industrial

waste• Produce biofuel for ~100 busses • Stable for decades at high

temperature• In-depth characterization


MetaproteomicsMetagenomics16S rRNA gene

Who is there? What’s their physiological potential?

What are they doing?

Whois doingwhat?


Hagen et al., Appl. Environ. Microbiol. January 2017

Having a Genome Sequence, We Can Understand a Microbe’s Metabolism

Plant scale Food Waste Reactor

Oslo EGE:• Receives food waste from the city

of Oslo• Produces biofuel and bio-fertilizer • First microbial characterization of

this plant, from start to stable


Achievements and Current Status

• From lab-scale studies to plant scale application

• Methodology: Functional analysis provides a more complete picture

• On a stage where we can start monitoring the process:–Study trends to foresee coming events–Detect the process problems at an earlier stage–Control the microbial community


Future Perspective• Still a lot unexplored!

• Down to basic: Design less complex lignocellulose-degrading communities–Can we discover novel enzymes and novel metabolic functions? –Learn from the Nature

• Apply in industry • Biogas production in a biorefinery context

–Anaerobic digestion of waste streams–Production of higher-value compounds


AcknowledgementsNMBU teamPEP/BTB group:Svein J. HornPhil B. PopeVincent G.H. Eijsink

Jeremy Frank Magnus Ø. ArntzenChris GabyMirzaman Zamanzadeh


Thank you for your attention!Contact: [email protected]

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Thank you for your attention!Contact: [email protected]

Biogas Production - SINTEF · PDF fileBiogas Production – Microbial Teamwork and...

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