Electrophysiological tests for the CNS

� Concept� Positioning

• High Quality Service• Central Nervous System• Functional Electrophysiology

Summary

• Functional Electrophysiology• High Skill Expertise

� Studies• Models• Portfolio• Scope

� Commitments� Benefits� Goal

NEUROSERVICE provides advanced tests and expertise in Functional Electrophysiology for the CNS :

� In vitro physiologyAcute Brain Slices recording : MEA, Patch-Clamp

Concept

� Acute Brain Slices recording : MEA, Patch-Clamp

� Standardized & Customised tests

[NBQX] in µM0.001 0.01 0.1 1 10 100

Nor

mal

ized

fEP

SP

0.0

0.2

0.4

0.6

0.8

1.0

1.2

n = 5 electrodes (one slice)

Positioning

FunctionalElectrophysiology

High QualityService

Central Nervous System

High SkillExpertise

High Quality Service

FlexibilityFlexibility

Expert supportExpert support

Short time Short time

processprocess

Reliability Reliability

of testsof tests

Quality InsuranceQuality Insurance

& Confidentiality& Confidentiality

The best quality ofService to satisfy

your needs

Central Nervous System

Acute Acute brainbrain

slicesslices

CNS disorders CNS disorders

Single neuron Single neuron TargetsTargets

A wide rangeof recordings

to fit your needs

Functional Electrophysiology

StandardizedStandardized

protocolsprotocols

CustomizedCustomized

protocolsprotocols

Mid troughoutputMid troughoutput MEA&PatchMEA&Patch

recording recording

The bestElectrophysiologyto empower yourscientific analysis

High Skill Expertise

ElectrophysiologyElectrophysiology Drug DiscoveryDrug Discovery

NeuroNeuro

PharmacologyPharmacology

NeuroNeuro

PhysiologyPhysiology

A High Expertise to fulfill your needs

� Molecules effects on neurons and neuronal networks� Targets & mechanisms of action� Interaction of a new molecule with an existing drug� Compound profiling

Studies

� Transgenic mice phenotyping� Consulting

[NBQX] in µM0.001 0.01 0.1 1 10 100

Nor

mal

ized

fEP

SP

0.0

0.2

0.4

0.6

0.8

1.0

1.2

n = 5 electrodes (one slice)

Models

� Hippocampal slices (glutamat- & GABAergic neurons in DG, CA3, CA1)� Cerebellar slices (Purkinje GABAergic large neurons)� Substantia Nigra (Dopaminergic neurons of pars compacta)compacta)� Cortex slices (glutamat- & GABAergic neurons in 5-6 layers)� Raphe nucleus (Serotoninergic neurons)� Thalamus & cortex� Striatum & brainstem� Spinal Cord

Portfolio Tests 1/3

Test # Reference compound Target Associated pathologiesP-001 Tetrodotoxin Sodium channel Epilepsy

Pain and Neuropathic PainHuntington’s DiseaseMultiple Sclerosis

P-002 NBQX AMPA/KA receptors StrokeEpilepsyAmyotrophic Lateral Sclerosis

P-003 D-AP5 NMDA receptor Alzheimer’s DiseaseP-003 D-AP5 NMDA receptor Alzheimer’s DiseaseParkinson’s DiseaseHuntington’s DiseaseSchizophrenia

P-004 Bicuculline GABA-A receptors EpilepsyAnxietySleep Disorders

P-005 Ganaxolone GABA-A receptors EpilepsyAnxietySleep disorders

P-006 Gabazin GABA-A receptors EpilepsyAnxietySleep Disorders

Portfolio Tests 2/3

P-007 DHPG mGluR 1/5 receptors StrokeEpilepsyNeurodegenerationAddictionAnxietyPain Management

P-008 Kainate AMPA/kainate receptors StrokeEpilepsyPainPainCognitive Dysfunctions

P-009 Nifedipine L-Type VDCC StrokeCognitive DysfunctionsEpilepsyDepression

P-010 Physostigmine Acetylcholinesterase Alzheimer’s DiseaseDementiaCognitive DysfunctionAttention Deficits Disorders

P-011 Memantine NMDA receptor Alzheimer’s Diseasenicotinic receptors Parkinson’s Disease

Dementia

Portfolio Tests 3/3

P-012 R-α-methylhistamine H3 histamine receptor Cognitive DisordersAlzheimer’s DiseaseParkinson’s DiseaseHyperactivity and Attention Deficit DisordersSleep DisordersObesityEpilepsy

P-013 Pyrilamine H1 histamine receptorsRanitidine H2 histamine receptorsRanitidine H2 histamine receptors

P-014 DCG-IV mGluR2 receptor Obsessive-Compulsive DisordersAnxiety/Stress DisordersDrug/Alcool Addiction

P-015 SCH23390 D1 dopamine receptor Cognitive DysfunctionD5 dopamine receptor Parkinson's Disease

P-016 Phenytoin K+ channels EpilepsyCarbamazepine

P-017 Carbamazepine NMDA receptor EpilepsyPhenytoin

P-018 Somatostatin GABA-A receptor Epilepsy

P-019 Diazepam GABA-A receptor Epilepsy

Scope

Addiction P-007, P-014Alzheimer’s Disease P-003, P-010, P-011, P-012Amyotrophic Lateral Sclerosis P-002Anxiety P-004, P-005, P-006, P-007, P-014Cognitive Disorders P-008, P-009, P-010, P-012, P-015Dementia P-010, P-011Depression P-009Depression P-009Epilepsy P-001, P-002, P-004, P-005, P-006, P-007,

P-008, P-009, P-012, P-016, P-017, P-018, P-019Huntington’s Disease P-003Hyperactivity and Attention Deficit Disorders P-010, P-012Multiple Sclerosis P-001Neurodegeneration P-007Neuropathic Pain P-001Obsessive-Compulsive Disorders P-014Pain P-001, P-007, P-008Parkinson’s Disease P-003, P-011, P-012, P-015Schizophrenia P-003Sleep Disorders P-004, P-005, P-006, P-012Stroke P-002, P-007, P-008, P-009

� Robust and reproduceable data

� High Quality service

� Short time processes

Commitments

� Short time processes

� Mid-throughoutput

� Expert support

� Confidentiality

Benefits

� Cost & Time saving

� Risk reducing

� Efficiency in scientific analysis

� Tests capacity

� Flexibility

Our Goal

To provide you with the best quality services in quality services in

Electrophysiological tests for the CNS to empower your

Science.