Nanoelectronic Scaffolding

By

Divya.A

Annalakshmi.A

•Nanoelectronic Scaffolding•Nanoelectronic-Tissue Construct•Formation of Tissues•Vascular Construct•Applications•Conclusion

The development of 3D synthetic tissues as structural and bioactive scaffold using macroporous nanowire and

nanoelectronic devices

Characteristics:•flexible • biocompatible.•macroporous

wire grid of silicon on a thin sheet of nickel the sheet is chemically dissolved, the wires fold up like origami into a three-dimensional scaffold shape organic polymer around nanoscale wires serve as the critical sensing elements. nanowire transistors to measure the activity in cells without damaging them.conventional planar nanoelectronics fabrication enable integration of additional device components

•Light blue: silicon oxide substrates•blue: nickel sacrificial layers•green: nanoES•yellow dots:nanowire FETs

•Yellow dots: nanowire components•blue ribbons: interconnects•pink: cells.

•step A:patterning & metallization –

Si nanowire & nanotransistor•step B:

forming 3D nanowire FET matrices (nanoES) •step C:

incorporation of cells and growth of synthetic tissue through biological processes.

microscopy image of one circular region of the nanoES, with nanowiretransistor at the center.

•Flexible mesh•rolled into tubular constructs and folded.•mesh porosity is comparable to that of a honeycomb•have nanoscale probes to avoid disruption of either cellular or tissue architecture.

3D reconstruction confocal microscopy image of a 3D macroporousnanoelectronic scaffold; a nanoelectronic device exists in each circular region

• substrate was dissolved, leaving a netlike sponge• folded or rolled into a host of three-dimensional shapes• networks were porous enough to allow to seed them with cells and encourage those cells to grow in 3-D cultures• we can work at the same scale as the unit of biological system without interrupting it.• merges tissue with electronics ”where the tissue ends and the electronics begin.”

Changes in conductance over time for two nanowire FET devices located in the outermost (red) and innermost (blue) layers. Outer tubing delivered bathing solutions with varying pH (red dashed lines and arrows); inner tubing delivered solutions with fixed pH (blue dashed lines and arrows).

Ability to Resolve Extravascular ph Changes Detects:

•distinct from either engineered tissue or flexible electronics.• Cell interactions with nanoES - growth determinants•incorporate nanoscale stimulators and stretchable designs - electrical and mechanical stimulation - cell culture.

•inflammation• ischaemia• tumour

Features of Vascular Construct:

•Epithelial tissue (skin)high surface to volume ratio,promotes the cell–matrix

interaction at the nanoscale

•Connective tissue(Bone ,ligaments,tendon, cartilage)high surface porosity, improves cell ingrowth and mechanical

properties of the scaffold

•Fluid tissue(blood ,thrombin,fibrinogen)improve the blood clotting process in wound healing

•Nerve tissue:enhances the conductivity of sensors via electron

transport which is extermly effective for nerve tissue scaffolds.

•Voluntary Muscle tissue(Arm,Leg,skeleton muscles )Electrospun nano fibres of polyester urethane has satisfactory mechanical property and encouraging cellular response in terms of adhersion ,they can be used in scaffolds for skeleton muscles.

•Involuntary muscle tissue(heart , intestine)Electrospun Small intestinal sub mucosa have a stable structure which provide improved hydrofilicity ,mechanical property and cellular behavior.

The nanoES merges the nanotechnology and electronics which finds its application in biomedical field that paves platform for curing the diseases

with the help of electronics and ultimately increasing the life expectancy of the human being.

Thank you