Molecular Biology of Genes Chapters 11-12 + DNA Technology (not in your book)
-
date post
22-Dec-2015 -
Category
Documents
-
view
215 -
download
2
Transcript of Molecular Biology of Genes Chapters 11-12 + DNA Technology (not in your book)
![Page 1: Molecular Biology of Genes Chapters 11-12 + DNA Technology (not in your book)](https://reader035.fdocuments.in/reader035/viewer/2022062715/56649d815503460f94a656f0/html5/thumbnails/1.jpg)
Molecular Biology of GenesChapters 11-12 +
DNA Technology (not in your book)
![Page 2: Molecular Biology of Genes Chapters 11-12 + DNA Technology (not in your book)](https://reader035.fdocuments.in/reader035/viewer/2022062715/56649d815503460f94a656f0/html5/thumbnails/2.jpg)
Double Stranded Helix
![Page 3: Molecular Biology of Genes Chapters 11-12 + DNA Technology (not in your book)](https://reader035.fdocuments.in/reader035/viewer/2022062715/56649d815503460f94a656f0/html5/thumbnails/3.jpg)
Nitrogenous Bases
![Page 4: Molecular Biology of Genes Chapters 11-12 + DNA Technology (not in your book)](https://reader035.fdocuments.in/reader035/viewer/2022062715/56649d815503460f94a656f0/html5/thumbnails/4.jpg)
![Page 5: Molecular Biology of Genes Chapters 11-12 + DNA Technology (not in your book)](https://reader035.fdocuments.in/reader035/viewer/2022062715/56649d815503460f94a656f0/html5/thumbnails/5.jpg)
So how do we copy it?
![Page 6: Molecular Biology of Genes Chapters 11-12 + DNA Technology (not in your book)](https://reader035.fdocuments.in/reader035/viewer/2022062715/56649d815503460f94a656f0/html5/thumbnails/6.jpg)
![Page 7: Molecular Biology of Genes Chapters 11-12 + DNA Technology (not in your book)](https://reader035.fdocuments.in/reader035/viewer/2022062715/56649d815503460f94a656f0/html5/thumbnails/7.jpg)
![Page 8: Molecular Biology of Genes Chapters 11-12 + DNA Technology (not in your book)](https://reader035.fdocuments.in/reader035/viewer/2022062715/56649d815503460f94a656f0/html5/thumbnails/8.jpg)
Genotype to Phenotype
![Page 9: Molecular Biology of Genes Chapters 11-12 + DNA Technology (not in your book)](https://reader035.fdocuments.in/reader035/viewer/2022062715/56649d815503460f94a656f0/html5/thumbnails/9.jpg)
Building Blocks of Proteins
![Page 10: Molecular Biology of Genes Chapters 11-12 + DNA Technology (not in your book)](https://reader035.fdocuments.in/reader035/viewer/2022062715/56649d815503460f94a656f0/html5/thumbnails/10.jpg)
![Page 11: Molecular Biology of Genes Chapters 11-12 + DNA Technology (not in your book)](https://reader035.fdocuments.in/reader035/viewer/2022062715/56649d815503460f94a656f0/html5/thumbnails/11.jpg)
![Page 12: Molecular Biology of Genes Chapters 11-12 + DNA Technology (not in your book)](https://reader035.fdocuments.in/reader035/viewer/2022062715/56649d815503460f94a656f0/html5/thumbnails/12.jpg)
![Page 13: Molecular Biology of Genes Chapters 11-12 + DNA Technology (not in your book)](https://reader035.fdocuments.in/reader035/viewer/2022062715/56649d815503460f94a656f0/html5/thumbnails/13.jpg)
tRNA
![Page 14: Molecular Biology of Genes Chapters 11-12 + DNA Technology (not in your book)](https://reader035.fdocuments.in/reader035/viewer/2022062715/56649d815503460f94a656f0/html5/thumbnails/14.jpg)
Ribosome StructureLarge Subunit
Small Subunit
![Page 15: Molecular Biology of Genes Chapters 11-12 + DNA Technology (not in your book)](https://reader035.fdocuments.in/reader035/viewer/2022062715/56649d815503460f94a656f0/html5/thumbnails/15.jpg)
![Page 16: Molecular Biology of Genes Chapters 11-12 + DNA Technology (not in your book)](https://reader035.fdocuments.in/reader035/viewer/2022062715/56649d815503460f94a656f0/html5/thumbnails/16.jpg)
![Page 17: Molecular Biology of Genes Chapters 11-12 + DNA Technology (not in your book)](https://reader035.fdocuments.in/reader035/viewer/2022062715/56649d815503460f94a656f0/html5/thumbnails/17.jpg)
Mutations
![Page 18: Molecular Biology of Genes Chapters 11-12 + DNA Technology (not in your book)](https://reader035.fdocuments.in/reader035/viewer/2022062715/56649d815503460f94a656f0/html5/thumbnails/18.jpg)
Sickle Cell Example
![Page 19: Molecular Biology of Genes Chapters 11-12 + DNA Technology (not in your book)](https://reader035.fdocuments.in/reader035/viewer/2022062715/56649d815503460f94a656f0/html5/thumbnails/19.jpg)
DNA Technology
![Page 20: Molecular Biology of Genes Chapters 11-12 + DNA Technology (not in your book)](https://reader035.fdocuments.in/reader035/viewer/2022062715/56649d815503460f94a656f0/html5/thumbnails/20.jpg)
Polymerase chain reaction (PCR)
- used to amplify DNA sequences
![Page 21: Molecular Biology of Genes Chapters 11-12 + DNA Technology (not in your book)](https://reader035.fdocuments.in/reader035/viewer/2022062715/56649d815503460f94a656f0/html5/thumbnails/21.jpg)
Gel electrophoresis sorts DNA molecules by size
+ +
– –
Powersource
Gel
Mixture of DNAmolecules ofdifferent sizes
Longermolecules
Shortermolecules
Completed gel
![Page 22: Molecular Biology of Genes Chapters 11-12 + DNA Technology (not in your book)](https://reader035.fdocuments.in/reader035/viewer/2022062715/56649d815503460f94a656f0/html5/thumbnails/22.jpg)
How Restriction Fragments Reflect DNA Sequence– Restriction fragment length polymorphisms (RFLPs)
reflect differences in DNA samples
Crime scene Suspect
w
x
y y
z
CutCut
Cut
DNA from chromosomes
C
C
G
G
G
G
C
C
A
C
G
G
T
G
C
C
C
C
G
G
G
G
C
C
C
C
G
G
G
G
C
C
x, y, z = restriction fragments
Red line =restriction site
![Page 23: Molecular Biology of Genes Chapters 11-12 + DNA Technology (not in your book)](https://reader035.fdocuments.in/reader035/viewer/2022062715/56649d815503460f94a656f0/html5/thumbnails/23.jpg)
– After digestion by restriction enzymes the fragments are run through a gel
–
+
Longerfragments
Shorterfragments
x
w
y
z
y
1 2Crime scene Suspect
w
x
y y
z
CutCut
Cut
DNA from chromosomes
CCGG
GGCC
ACGG
TGCC
CCGG
GGCC
CCGG
GGCC
![Page 24: Molecular Biology of Genes Chapters 11-12 + DNA Technology (not in your book)](https://reader035.fdocuments.in/reader035/viewer/2022062715/56649d815503460f94a656f0/html5/thumbnails/24.jpg)
DNA fingerprinting can help solve crimes
Defendant’sblood
Blood fromdefendant’s clothes
Victim’sblood