Mining the Antibodypedia

30
Lars Juhl Jensen Mining the Antibodypedia Research barely in progress seminar

Transcript of Mining the Antibodypedia

Page 1: Mining the Antibodypedia

Lars Juhl Jensen

Mining the Antibodypedia

Research barely in progress seminar

Page 2: Mining the Antibodypedia

web resource

Page 3: Mining the Antibodypedia

commercial antibodies

Page 4: Mining the Antibodypedia

Antibodypedia AB

Page 5: Mining the Antibodypedia

Nature Publishing Group

Page 6: Mining the Antibodypedia
Page 7: Mining the Antibodypedia

query for a protein

Page 8: Mining the Antibodypedia
Page 9: Mining the Antibodypedia

Creative Commons

Page 10: Mining the Antibodypedia

no download file

Page 11: Mining the Antibodypedia

web robot

Page 12: Mining the Antibodypedia

scraped all data

Page 13: Mining the Antibodypedia

mapped identifiers

Page 14: Mining the Antibodypedia

STRING

Page 15: Mining the Antibodypedia
Page 16: Mining the Antibodypedia

579,038 antibodies

Page 17: Mining the Antibodypedia

16,827 human proteins

Page 18: Mining the Antibodypedia

variable quality

Page 19: Mining the Antibodypedia

rated for various uses

Page 20: Mining the Antibodypedia

Western blot

Page 21: Mining the Antibodypedia

ELISA

Page 22: Mining the Antibodypedia

immunoprecipitation

Page 23: Mining the Antibodypedia

immunoflourescence

Page 24: Mining the Antibodypedia

immunohistochemistry

Page 25: Mining the Antibodypedia

flow cytometry

Page 26: Mining the Antibodypedia

combine these ratings

Page 27: Mining the Antibodypedia

unified quality score

Page 28: Mining the Antibodypedia

quality scores

Page 29: Mining the Antibodypedia

Western blot

flow cytometry

immunoflourescence

immunohistochemistry

immunoprecipitation

ELISA

immunocytochemistry

protein array

gel shift

chromatin immunoprecipitation

proximity ligation assay

blocking/neutralizing

functional assay

radioimmunoassay

immunoelectron microscopy

Page 30: Mining the Antibodypedia

thank you!