Meros High Speed Digital Microscope - Dolomite...MAR-000266_v.1.11 Meros High Speed Digital...

Contents 1 Introduction ...................................................................................................................................... 3

2 Safety .............................................................................................................................................. 4

Meros High Speed Digital Microscope

USER MANUAL

Version: 1.11

Author: AM

Date: 14/01/2015

P/N: MAR-000266 (3200531)

MAR-000266_v.1.11 Meros High Speed Digital Microscope User Manual Chapter: Introduction

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1 Introduction ...................................................................................................................................... 3

2 Safety .............................................................................................................................................. 4

3 Using the High Speed Digital Microscope ....................................................................................... 6

Overview ................................................................................................................................. 6

Box Contents ........................................................................................................................... 8

Assembly Instructions ............................................................................................................. 9

Installation of Flow Control Centre Software ......................................................................... 12

4 Operating the Microscope ............................................................................................................. 13

Physical Microscope Adjustments......................................................................................... 13

Flow Control Centre Microscope Settings ............................................................................. 16

4.2.1 Individual Icons Function ............................................................................................... 16

5 Support .......................................................................................................................................... 17

Servicing ................................................................................................................................ 17

Cleaning ................................................................................................................................ 18

FAQ ....................................................................................................................................... 18

6 Appendices.................................................................................................................................... 20

Appendix A PC Requirements .............................................................................................. 20

Appendix B CE Declaration of Conformity ............................................................................ 21

Appendix C Technical Specifications .................................................................................... 22

MAR-000266_v.1.11 Meros High Speed Digital Microscope User Manual Chapter: Introduction

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1 Introduction

Thank you for purchasing the Meros High Speed Digital Microscope System

from Dolomite Microfluidics.

These instructions have been carefully prepared to guide the installer and

end-user through the installation and use of the High Speed Digital

Microscope System.

Before attempting to use this product, please read this manual thoroughly

and follow the instructions carefully. In doing so you will ensure the safety of

yourself and that of others around you, and you can look forward to your

purchase giving you long and satisfactory service.

Please keep the manual easily accessible and in immediate vicinity of the

module. Only suitably qualified and trained personnel should operate this

unit.

The High Speed Digital Microscope System is a high quality and flexible

solution for general microscopy and high speed image capture in microfluidic

applications. It features an easy-to-use microscope with wide zoom range

and long working distance. The microscope stage is designed to

accommodate all types of microfluidic chips and enables users to quickly

locate and observe the area of interest.

MAR-000266_v.1.11 Meros High Speed Digital Microscope User Manual Chapter: Safety

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2 Safety

Warning and safety information

Do not look directly at the light coming from the lens of the

Microscope or from the reflection from the light control stage. It is

extremely bright and may cause eye damage.

The camera housing may be hot, avoid direct contact especially

after long periods of use.

The Microscope has an uneven weight distribution, take care

during assembly and if moving.

Do not cover the instrument during use.

This equipment should only be used by competent, suitably trained

personnel after they have read and understood this manual, and

considered any hazard involved.

Place the instrument on a solid horizontal surface. Ensure the area

around the unit is clear.

Adequate protection including appropriate PPE and ventilation

must be provided if hazardous chemicals are to be used in

conjunction with this unit. In the case of accidental spillage,

carefully wipe with a dry cloth, taking into account the nature of the

spilled liquid and the necessary safety precautions.

Comply with all safety and accident prevention regulations

applicable to laboratory work.

Always connect the instruments to an earthed AC power outlet.

The operating voltage is indicated on the specification sticker. Non

observance of this provision may result in damage to the

Microscope or in personal injury or damage to property.

This product does not comply with the ATEX directive and must

not be used in explosive atmospheres.

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MAR-000266_v.1.11 Meros High Speed Digital Microscope User Manual Chapter: Safety

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Disassembling any part of the Microscope may invalidate the

warranty.

Cleaning

Cleaning should only be performed by personnel trained in such

work, and who are aware of the possible dangers involved. The

microscope (and all the associated hardware) has not been

designed for sterilisation or use with an autoclave.

Returning Equipment

Equipment which has been contaminated with, or exposed to, body fluids,

toxic chemicals or any other substance hazardous to health must be

decontaminated before it is returned to Dolomite-Microfluidics or its

distributor.

Environmental Conditions

For indoor use only

Temperature range: 5°C to 40°C

Humidity: Maximum relative humidity of 80%

MAR-000266_v.1.11 Meros High Speed Digital Microscope User Manual Chapter: Using the High Speed Digital Microscope

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3 Using the High Speed Digital Microscope

Overview The Meros High Speed Digital Microscope is designed specifically for microfluidics and is ideal for observing droplets during high speed droplet production. The high speed, high resolution imaging and the convenient stage greatly simplify microfluidic experiments. The easy to use stage securely holds a wide range of microfluidic equipment for reliable viewing including microfluidic chips and. With the benefit of extra-long working distance, the High Speed Digital Microscope enables optical access to samples which would normally be difficult to view. Illumination from above leaves the underside of the microfluidic device free for temperature control and is compatible with the Meros TCU-100 (part no. 3200428). High magnification optics and the zoom lens ensure that micro-scale features can be clearly visualised. The integrated coaxial lighting system offers exceptional brightness for image capture at very low exposure times. Coupled with the high speed USB3.0 camera, the lighting and optics provide the capability to capture still images or videos of microfluidic material moving at kHz rates. The High Speed Digital Microscope is fully compatible with the Flow Control Centre software (downloadable for free from Dolomite website) which enables observation, image adjustment and image capture. Flow Control Centre also includes advanced functions (accessible with a purchased licence key) which allows complex protocols to be run with the capability to control Dolomite imaging systems, pumps, sensors and temperature control devices simultaneously”. Figure 1 shows the fully assembled High Speed Digital Microscope System


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Figure 1

Camera

Adjustable

Stage Optics

Cooling Fan

Light

Control

Stage


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Box Contents When you open the box containing your High Speed Microscope, ensure

that you have all of the following parts;

Component Image

Assembly comprising high speed digital microscope, mount, USB 3 cable and light control stage cable

Base

Upright

3 x Allen screws

1 x Allen key

1 x Light control stage


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1 x Double-sided light diffusor/reflector glass

2 x Chip clips

1 x Power supply with power cord

Assembly Instructions Screw the upright post into the base using the 3 Allen

screws and Allen key provided. See Figure 2.

Fit the microscope assembly onto the stand and tighten the

lock knob. See Figure 3.

Figure 2

Figure 3


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Position the light control stage under the microscope, plug

the power supply/power cord into the light control stage. See

Figure 4.

Plug the light control stage cable coming from the

microscope into the socket on the light control stage. See

Figure 5.

Plug the USB 3 cable from the microscope into your

PC/laptop.

Fit the diffusor/reflector glass on the top of the light control

stage. See Figure 6.

NOTE: The best performance will be achieved when the microscope is plugged into a USB 3 socket. If

plugged into a USB 2 socket, performance will be reduced as the data transfer rate is slower with this

set up.

Figure 4

Figure 5

Figure 6


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Fit the chip clips if required. The clips screw into a choice of

positions on the deck of the light control stage to allow for a

variety of possible configurations. See Figure 7.

An example of a chip held securely is shown below.

See Figure 8

Figure 7

Figure 8


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Installation of Flow Control Centre Software

The High Speed Microscope can be controlled via our Flow Control Centre

software. This is available to download FOC from the Dolomite Microfluidics

downloads area, http://www.dolomite-microfluidics.com/en/downloadsmain

and click on “software downloads” from the drop-down menu. In order to

download this, you must register on the website, it only takes a few minutes

and no credit card details are required.

Click on the downloaded file and follow the on-screen instructions.

Attach the microscope to your PC via the USB 3 cable.

For user instructions on how to use this software, select ‘help’ from

the top menu bar.

Your High Speed Digital Microscope is now ready to use.

NOTE: Flow Control Centre Software is only compatible with Windows 7.

NOTE: Please note that versions of FCC earlier than 3.x.x are not compatible

with this microscope.

NOTICE: When starting up your PC, the USB lead between the Microscope and the PC MUST be

disconnected. This applies even if the Microscope is turned off. Failure to do this may result in the

Microscope not being recognised as a USB device and will not be able to communicate with the Flow

Control Centre Software.

NOTE: We have a Droplet Monitoring Software that will allow you to analyse droplets from a saved

.avi file. The link below will take you to our software downloads page. Please take care to download

the correct version for your PC as 32 and 64 bit versions are available. http://www.dolomite-

microfluidics.com/webshop/software_downloads

You will require a licence to run this software. Please contact our support team support@dolomite-

microfluidics.com to request a licence key.

http://www.dolomite-microfluidics.com/en/downloadsmain

http://www.dolomite-microfluidics.com/webshop/software_downloads




mailto:[email protected]


MAR-000266_v.1.11 Meros High Speed Digital Microscope User Manual Chapter: Operating the Microscope

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4 Operating the Microscope

Physical Microscope Adjustments Coarse Height Adjustment

Whilst supporting the microscope, turn the lock knob anti-clockwise

to loosen the Microscope. With the Droplet Monitor Software

running, lower or raise the microscope to the required height so that

it is roughly in focus. See Figure 9.

Fine Height Adjustment

NOTE: You also have access to additional software tools including Microfluidic Flow Calculator and

Droplet Volume Calculator, to help you set up your microfluidics system. Contact Support@dolomite-

microfluidics.com if you require any of these aids.

Figure 9






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Moving either of the Fine Height Adjustment knobs will allow

the Microscope to be raised or lowered with fine control to

achieve a sharp focus. See Figure 10.

Tension Adjustment

The force required to move the fine

adjustment knob can be varied. Hold the

right hand knob (plain) still whilst adjusting

the left hand knob (marked) clockwise to

increase the tension, or anti-clockwise to

reduce the tension. See Figure 11.

Figure 10

Adjust

Hold

Figure 11


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Magnification Adjustment

Adjust the dial to the mark to change the magnification, 1 is the

lowest magnification, 5 is the highest. See Figure 12.

Light source Adjustment

Adjust the dial to change the brightness at the light control

stage. See Figure 13.

Securing the chip

Position the clips to suit the chip. Push down of the plastic

arm of the clip to secure the chip. See Figure 14.

Figure 12

Figure 13

Figure 14


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Flow Control Centre Microscope Settings Within Flow Control Centre, click on the “Devices” tab to access a

Microscope specific and the pop-up window, as seen below.in Figure 15.

This window gives access to parameters that will change the

appearance and quality of the image that can be captured. There are

8 Icons in total that allow access to the control settings, image capture

settings, file save settings and Region of Interest (ROI) selection.

4.2.1 Individual Icons Function

Pop-Up

Opens a screen where just the live image can be seen

without clutter.

Settings

Opens a new window, see Figure 16 below.

Image

Click to capture a single image.

Video

Click to capture video, time can be set between 1 and 600

seconds.

Open

Open the default folder where images are stored.

Change

Change the default folder where images are stored.

Figure 15

MAR-000266_v.1.11 Meros High Speed Digital Microscope User Manual Chapter: Support

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ROI

Click and draw a box on the image window to select the ROI.

Re-set ROI

Click to re-set the ROI to full size.

Exposure Time

This is the equivalent to shutter speed in a conventional camera.

Click on "Exposure" slider and drag to obtain the desired

exposure time setting. We suggest that you initially select the

shortest possible 0.05ms (50µs). The sharpest images will be

taken with the smallest exposure time and this is especially true

when looking at droplets being produced in the order of kHz.

Alternatively, select the tick box to automatically determine the

exposure time

Gain

This is the equivalent to film speed in a conventional camera.

This can be useful when lighting levels are low. Increasing the

gain boosts the signal and hence appears brighter. However, it

should only be necessary to adjust this setting if working under

low light conditions or with high speed droplet production. Please

note the increasing the gain will also increase noise levels

causing the image to be less clear.

Frame Rate

This is the number of frames captured per second (FPS) during

video capture. Increasing the FPS will slow down the apparent

movement of the droplets.

Gamma

This setting increases contrast e.g. improves the definition.

Image Flip

Depending on the Droplet Imaging Software you are using, this

allows the direction of the droplet flow to be oriented correctly. Our

Droplet Monitor Software requires that the droplets are moving from

left to right.

Region of Interest (ROI)

Use this feature to select the region that you wish to view/capture.

Note that the larger ROI, the larger the file size and the lower the

frames/second rate.

Undo Region of Interest

Returns the ROI to full screen.

Quick Start Summary

Set the exposure time as low as possible, 0.05ms

recommended.

Increase the lighting brightness.

If more light is still required, increase the gain.

5 Support

Servicing There are no user serviceable parts in the high speed microscope.

Figure 16


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The LED light source brightness will be reduced over a period of time. We

suggest that your microscope is refurbished when the brightness too low for

normal use (approximately 3000 hours of use). Please contact

[email protected] for further details.

Cleaning The lens can be gently wiped using a lint free wipe (clean room wipe).

Cleaning liquids should not be used as these can damage the optical coating

on the lens.

All other components of the microscope and should be kept free of dust and

fibres. Disconnect the microscope from the mains power supply before

cleaning and also ensure that is completely cool. The surfaces can be

cleaned with a lint free wipe and a small amount of iso propyl alcohol.

FAQ Q. All I can see is a white screen, what do I need to change?

A. There are 3 settings that could cause the screen to appear white, you will

need to adjust 1 or more of these to obtain the desired lighting level.

The exposure is too high, click on the auto button or manually select a lower

value. The recommended starting value for exposure time is 0.05ms

The light source is to bright, reduce the brightness adjustment knob.

The gain is too high, select a lower value.

Q. My image is too dark, and I can’t see droplets. What can I

do?

A. Increase the light to maximum, and increase gain until image is bright

enough.

Q. My chip won’t sit properly on the stage.

A. You can clip the chip on with the clips on the stage (see Figure 11 above).

If the clips are missing, you can order replacements (3200430).

Q. I think there should be droplets, and everything seems fine,

but I can only see a blur or lines at the junction.

A. There are several possible causes.

The droplets may actually be blurred. First, decrease the exposure time to

the minimum (0.05ms), and adjust the image so you have a bright, focussed

image.

Check that the image is live (communication hasn’t ‘hung’) by moving the

microscope state – the image should also move.

If you can see what looks like jetting flow through the junction, decrease first

the droplet phase flow rates, and then the carrier phase until the jetting

changes to ‘dripping’.



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Check that you actually have flow going through the chip. If there is a

blockage, check the chip, fittings and tubing until you find the blockage.

Q. My droplets appear to be moving backwards, why is that?

This is a stroboscopic effect due the number of Frames per Second and the

Hz rate of the droplets being produced. You may have seen this before in a

movie where the propeller of a plane appears to move backwards. You can

change whether the droplets appear to move forwards, backwards or remain

stationary by adjusting the Frames per Second. This will not affect the

quality of the data.

MAR-000266_v.1.11 Meros High Speed Digital Microscope User Manual Chapter: Appendices

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6 Appendices

Appendix A PC Requirements Component Requirement

CPU Minimum: 2.0 GHz Intel Pentium Dual Core compatible

Recommended: 3.5 GHz + Intel Pentium Dual Core compatible or

better

Memory Minimum: 4GB RAM

Recommended: 8GB or 16GB

Operating System Windows 7 Professional or better (32 and 64 bit)

Recommended: Fresh OS Installation with all the latest Windows

updates (Preferably not from a disc image).

Blacktrace software is not Windows 8 or 10 compatible

Display Resolution Minimum: Super VGA 1024 x 768

Recommended: Super VGA 1920 x 1080 or better.

Video Card: 24-bit or 32-bit with at least 64MB of video memory.

Native USB Ports Varies based on system, but the minimum is 1 x USB 3 ports and 3

x USB ports. We strongly recommend all Blacktrace hardware is

connected directly to the PC.

Additional USB ports can be added to a PC using only Blacktrace

approved powered USB Hubs.

USB Cables Shielded USB Cables can be no more than 3 meters in length from

PC to device.

Third Party Software The PC must be dedicated for Blacktrace software and no third

party software should be installed.

Anti-Virus Recommended: Symantec

Other Anti-Virus and Firewall software can prevent the Blacktrace

software from running properly

Regional Settings ‘UK English’ must be selected in Regional Settings, not just

language settings

Further Options:

RS232: For some hardware a USB to COM port converter is required.

Please contact [email protected] for further information.

Support If you require further details or advice please contact

[email protected]



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Appendix B CE Declaration of Conformity

We The Dolomite Centre Ltd

of 27 Jarman Way, Royston

in accordance with the following Directive(s):

2004/108/EC The Electromagnetic Compatibility Directive

2006/95/EC The Low Voltage Directive

hereby declare that: Equipment: MEROS HIGH SPEED DIGITAL MICROSCOPE:

Part Number Description

3200531 MEROS HIGH SPEED DIGITAL MICROSCOPE

and associated accessories are in conformity with the applicable requirements of the following documents:

Ref. No. Title Edition/date

EN ISO 12100 Safety of machinery — General principles for design — Risk assessment and risk reduction

2010

EN 61010-1 Safety requirements for electrical equipment for measurement, control, and laboratory use. General requirements

2010

EN 61326-1

EN 61000-3-2

EN 61000-3-3

EN 62471

Electrical equipment for measurement, control and laboratory use. EMC requirements

EMC – harmonic current emissions

EMC – voltage changes, voltage fluctuations and flicker in public low-voltage supply systems

Photobiological safety of lamps and lamp systems

2013

2014

2013

2008

I hereby declare that the equipment named above has been designed to comply with the relevant sections of the above referenced specifications. The unit complies with all applicable essential requirements of the Directives.

Signed by:

Name: Andrew Lovatt

Position: CTO

15

Done at The Dolomite Centre Ltd 27 Jarman Way Royston, Hertfordshire SG85HW, UK 18/12/2015


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Appendix C Technical Specifications

Specification

Minimum field of view (maximum zoom) 0.26 x 0.19 mm (approx.)

Maximum field of view (minimum zoom) 1.8 x 1.3 mm (approx.)

Objective lens magnification 5X

Zoom ratio 7:1

Working distance 45 mm

Number of pixels per micron at maximum zoom

5

LED Luminous Flux (1) 4000 lumens

LED colour Cool white

LED wavelength 400 – 800 nm

Imaging device 1.3 Megapixel colour camera, 1/2” CMOS sensor

Resolution 1280 x 1024 pixels

Minimum Shutter time 0.05 ms

Frame rate (2)

~150 fps @ 1280 x 1024 ~506 fps @ 640 x 480

Still image file type .bmp

Video image file type .avi

PC connection USB 3.0

System dimensions (3) 260 x 318 x 490 mm (W x L x H)

Stage dimensions 107 x 187 x 52 mm (W x L x H)

Supply voltage and frequency 80 – 264 VAC, 47 – 63 Hz


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