Measuring Amounts of Vitamin C in Apple Juice

Leah Bury, Grade 9

• Using titration to find out which type of apple juice (from concentrate, not from concentrate) contains the most vitamin C.

• Chose this experiment in order to see if there was a significant difference between types of apple juice

Problem

• Titration- method that uses a solution with a known concentration to find the concentration of a second solution.

• Vitamin C- apple juice is not a natural source

Research

Hypothesis

• If two different types of apple juice differ in ingredients and/or type, then they will have different amounts of Vitamin C.

Materials• Apple Juice (20 mL)

• Apple Juice from Concentrate (20 mL)

• Vitamin C Tablets (125 mg)

• Distilled Water

• Iodine Solution (50 mL)

• Soluble Starch (.125 g)

Variables•Independent- type of apple juice

used

•Dependent- amount of vitamin C

•Controlled- concentration of Iodine solution

Procedure

• First, Iodine and distilled water were mixed to create a 25% Iodine solution.

• A starch indicator solution was made by adding .125 g of starch to 25 mL of distilled water.

• A vitamin C standard solution was made by mixing 125 mg of vitamin C with 50 mL of distilled water.

• The standard vitamin C solution was titrated by adding 3 drops of the starch indicator and measuring the amount of Iodine solution needed to turn the vitamin C solution black.

Procedure• Then 20 mL of apple juice was titrated.

• After that, 40 mL of apple juice from concentrate was mixed with 40 mL of distilled water.

• 20 mL was titrated.

Data

Vit. C Solution 0.06 M

Apple Juice 0.3 M

Concentrate 0.1 M

Amt. of Vit. C

Conclusion• If two different types of apple juice differ in ingredients and/or type, then

they will have different amounts of Vitamin C.

• The hypothesis was supported: the 2 different types of apple juice contained different amounts of vitamin C.

• The apple juice (not from concentrate) contained the most vitamin C. The vitamin C solution contained the least vitamin C.

• Apple juice not from concentrate does not have any water added.

Further Experimentation

•Experiment can be carried further by retesting and measuring the vitamin C in other fruit juices.

Thanks for listening!

• Sources:

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