M.C.Q

Objective: To prepare a temporary of a leaf peel to show stomata.

1. While preparing a temporary stained mount of a peel, the extra stain from the slide is removed bya> Washing with water b> Using a dropperc> Soaking with filter paperd> Absorbing with cotton

2. The stain used in the preparation of temporary mount of a leaf peel to observe stomata is :a> Safranin b> Methylene bluec> Glycerine d> Iodine solution

3. A temporary slide of a leaf peel is mounted in :a> Iodine b> Glycerine c> Safranin d> Methylene blue

4. The stomatal opening is controlled by guard cells. If the stomatal opening is to remain open how should the guard cells be?a> Stretched b> Swollenc> Rigid d> Shrunk

5. The guard cells are also capable of photosynthesis because they havea> Cytoplasm, nucleus, centriolesb> Cytoplasm, nucleus, centrosome c> Cytoplasm, nucleus, chromosomesd> Cytoplasm, nucleus, chloroplasts

6. The steps involved in making a slide of epidermal peel of leaf are given as follows :I. Pull out a thin peel from the lower surface of the leaf.II. Place a drop of glycerine on the slide.III. Stain the peel in safranin.IV. Place the stained peel of the glycerine.V. Remove the extra stain by washing with water.VI. Place the cover slip over the peel.

Which one is the correct sequence of steps to be followed?a> I, II, III, IV, V, VIb> I, III, V, II, IV, VIc> I, III, IV, II, V, VId> I, II, IV, III, V, VI

7. A well stained leaf peel mount when observed under the high power of a microscope shows nuclei in:a> Only epidermal cellsb> Only guard cellsc> Guard cells and epidermal cellsd> Guard cells, epidermal cells and stoma

8. To prepare a good temporary mount of the petunia leaf peel showing many stomata, the student has to get the peel from thea> Tip of the leaf.b> Upper surface of the leaf.c> Lower surface of the leaf.d> Point of attachment of the leaf to its petiole.

9. The temporary mount of the leaf epidermal peel which looked pinkish red under the microscope wasa> Stained in acetocarmine and mounted in glycerine.b> Stained in iodine and mounted in glycerine.c> Stained in safranin and mounted in glycerine.d> Stained in methylene blue and mounted in water.

10. Which is the correct way to observe guard cells and their nuclei under the microscope?a> Use coarse adjustment to focus them under low power.b> Use fine adjustment to focus them under low power.c> Use coarse adjustment to focus them under high power.d> Use fine adjustment to focus them under high power.