MCB 7200 Group 1 Ashley Patton Yanyang Cuo Samantha Shaw.
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Transcript of MCB 7200 Group 1 Ashley Patton Yanyang Cuo Samantha Shaw.
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MCB 7200
Group 1Ashley PattonYanyang CuoSamantha Shaw
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Biological Relevance◦ Repairs double-stranded breaks◦ Chromosome segregation during meiosis I◦ Genetic diversity in gametes
Mediated by two important enzymes◦ Rad51 and Dmc1
Occurs during late-S phase, G2, M
Filippo et al. 2008. Mechanism of Eukaryotic Homologous Recombination
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Double Strand Break Repair
Synthesis-dependent strand annealing ◦ Migrating D loop◦ No Holiday Junction formation◦ Mitosis/Meiosis
Single strand annealing◦ Repair of double strand break by deletion of repeated DNA
sequences ◦ No Holiday Junction formation
Break-induced repair◦ Telomeres ◦ Replication forks
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Filippo et al. 2008. Mechanism of Eukaryotic Homologous Recombination
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RecBCD◦ Helicase activity unwinds
DNA◦ Exonuclease activity
degrades both single strands◦ At CHI site
3′ - 5′ Exonuclease activity is inhibited
5′ -3′ activity enhanced yielding a single-stranded 3′ end. This end becomes coated
with multiple RecA proteins, which catalyze strand invasion and formation of a Holliday structure
Lodish et al., Molecular Cell Biology, 4 th Fig 12-32
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RecA◦ RecA binds ssDNA in the presence of ATP◦ RecA aligns the ssDNA with its
homologous dsDNA region◦ RecA inserts the ssDNA into target DNA
sequence forming a heteroduplex Holiday-type structure
Branch Migration and Resolution by RuvA,B,C
Lodish et al., Molecular Cell Biology, 4 th Fig 12-35
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http://www.qiagen.com/products/genes%20and%20pathways/pathway%20details.aspx?pwid=143
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http://www.qiagen.com/products/genes%20and%20pathways/pathway%20details.aspx?pwid=143
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Filippo et al. 2008. Mechanism of Eukaryotic Homologous Recombination
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Used for double strand break repair and genetic diversity in meiosis.
Different mechanisms within the Eukaryotic and Prokaryotic cells.
Proteins are different with each system by have similar functions.
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C. Wyman et al. /DNA Repair 3 (2004) 827–833
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Fillipo et al. Mechanism of Eukaryotic Homologous Recombination. 2008. Annual Rev. Biochem. 77:229-57.
Lodish et al. Molecular Cell Biology. 1999. W. H. Freeman. 4th edition. Oldenburg et al. Recombination-mediated PCR directed plasmid construction in
vivo in yeast. 1997. Nucleic Acids Research. Vol. 25, No. 2. Wyman et al. Homologous recombination-mediated double-strand break repair.
(2004) DNA Repair. 3:827–833.
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