Cod Reproductive Ecology: Effect of dietary fatty acids on ovarian maturation, spawning

time and quality of eggs and larvae.

Maria C. Røjbek, PhD student, DTU Aqua, 2008-2011Bornholms Lakseklækkeri, Øster Flak 2, DK-3730 Nexø

Josianne Støttrup, Section for Coastal EcologyJonna Tomkiewicz, Section for Population- and Ecosystem DynamicsCharlotte Jacobsen, Section for Aquatic lipids and Oxidation

Aim of the Phd studyBaltic cod food chain and the reproductive success•Lipid dynamics in cod•Lipid dynamics in sprat and herring•Lipid dynamics in plankton

Comparisons between ecosystems, and between wild and captive cod fed dry feed with different lipid composition

Effects of fatty acid composition on spawning time and egg- and larval quality

Improvement of egg and larvae quality in cod farming through enhanced broodstock feed

Declined reproductive success of Baltic cod

Köster F.W. et al., ICES Marine Science Symposia, 219: 294-306. 2003

Collection of Baltic cod, sprat, herring and zooplankton in the Central Baltic Sea

XXX

X

###

####

###

##

#

# ##^^^

^^^

^^^

^

^

((

((((

(((

((((

(( (

( ((( (((

(

GG

""

"""""

"

""

"""

""""

""

"

"

"

!

!

!!!!

!

!!

!!

!!!!!!!

IVa

IVb

IVc

VIIa

VIa

VIa

Vb

IIa

20

21

22

23

24

25

27

26

28

29

30

15°E

15°E

10°E

10°E

5°E

5°E

0°

0°

5°W

5°W

60

°N

60

°N

55

°N

55

°N

Cruise

X Heincke Jan. 2003

# Dana Nov. 2003

^ Dana Jan. 2004

( Dana Mar. 2004

G Alkor Jun. 2004

" Scotia Jan. 2004

! Dana Feb. 2004

Histological analyses of cod gonads

Fatty acids in cod ovary and liver in relation to maturity stage of gonads

-1.0

-0.5

0

0.5

1.0

-1.0 -0.5 0 0.5 1.0X-expl: 21%,11%

Length

MII + IX

MIIIMIII0

MIII1 MIII2

MIV

HSI

GSI

Lipid

14:014:115:016:0

16:1(n-7)

16:2(n-4)

17:0

16:3(n-4)

17:1(n-7)

16:4(n-3)

18:0

18:1(n-9)

18:1(n-7)

18:2(n-6)

18:2(n-4) 18:3(n-4)

18:3(n-3)18:4(n-3)

20:1(n-9)

20:1(n-11)

20:2(n-6)

20:3(n-6)ARA

20:3(n-3)

20:4(n-3)

EPA22:1(n-11)

22:1(n-9)

21:5(n-3)

22:5(n-3) DHA

24:1(n-9)

SFA

MUFA

PUFA

n-6 PUFA

n-3 PUFA

n-3/n-6

EPA/ARA

DHA/EPA

PC1

PC2

Correlation Loadings (X)

-1.0

-0.5

0

0.5

1.0

-1.0 -0.5 0 0.5 1.0X-expl: 21%,11%

Length

MII + IX

MIIIMIII0

MIII1 MIII2

MIV

HSI

GSI

Lipid

14:014:115:016:0

16:1(n-7)

16:2(n-4)

17:0

16:3(n-4)

17:1(n-7)

16:4(n-3)

18:0

18:1(n-9)

18:1(n-7)

18:2(n-6)

18:2(n-4) 18:3(n-4)

18:3(n-3)18:4(n-3)

20:1(n-9)

20:1(n-11)

20:2(n-6)

20:3(n-6)ARA

20:3(n-3)

20:4(n-3)

EPA22:1(n-11)

22:1(n-9)

21:5(n-3)

22:5(n-3) DHA

24:1(n-9)

SFA

MUFA

PUFA

n-6 PUFA

n-3 PUFA

n-3/n-6

EPA/ARA

DHA/EPA

PC1

PC2

Correlation Loadings (X)

Lipid and fatty acid dynamic in clupeids in relation to sex, maturity stage and season

Seasonal variation in sprat

Bornholms Lakseklækkeri

St. Andrews Biological Station

Egg qualityFatty acid composition

Pigment analysis

Fertilisation rate

Hatching rate

Survival rate

Sperm qualityFatty acid composition

Motility

Viability

Larvae qualityFatty acid composition

Viability

Resistens to stress

Thank you for your attention