Manual Tecnico 290

7/23/2019 Manual Tecnico 290

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3MAttestAuto-reader

Service Manual

Health Care

Neither this book nor any part may be reproduced or transmitted in any form or by any means, electronic or mechanical, including

photocopying, microfilming, recording or by any information storage and retrieval system, without permission from 3M Health Care.


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Attest Auto-reader Service Manual

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Revision History

Revision Date Page(s) Changed

A 10/16/2001 All

Change: Birth Notice, Manual Released

B 7/16/2003 Deleted Pages 5-13

Updated New Pages 5-14

Updated New Pages 16-21

Change: Eliminated Operators Manual Section

Change: Updated Acceptance Test Procedure to match C-05-005002 (MCU-0002) Version 7Change: Updated BOMs to designate spare parts availability

C 7/18/2005 All

Change: Added drawings and parts list for new 290 case designChange: Added drawings and parts list for 293/293G Auto-reader

Change: Updated Acceptance Test Procedure to include 293/293G Auto-reader

D 4/21/2009 All

Change: Performed an 11-digit stock number review, update some per ECOs.Change: Added new round cord clip for power supply, 26-1017-3696-0 and deleted the flatpower supply cord clip and screw, 26-1017-2681-3, 26-1014-7689-8, Reference, ECO-05-003550.

Change: Changed the 10 inch ribbon cable (connects the NMOM and display PCB) from 26-1014-

7284-8 to the 12 inch ribbon cable 26-1017-6304-8 for the 293/293G, reference ECO-05-003152.Change: Added Connectivity Kit stock numbers to the 290 and 290G per ECO-05-003862.

Change: Added stand-offs, Dual Lock, etc. to Service Manual (for shipping purposes for the

293/293G), reference ECO-05-003345.


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TABLE OF CONTENTS

TROUBLESHOOTING GUIDE

Troubleshooting Guide . 5

ACCEPTANCE TEST PROCEDURE

Special Instructions 7

Assembly/Test Procedure 7

Acceptance Test Procedure 8Calibration Procedure 10

Preshipment checks/Packout Procedure 11

290/290G Acceptance Test Sheet 13

293/293G Acceptance Test Sheet 15

ILLUSTRATED PARTS LIST

290/290G Assembly 18

293/293G Assembly 22Frame Assembly 24

Sled Assembly 26

Sled Holder 27

THEORY OF OPERATION

Detailed Discussion 28

SCHEMATIC DIAGRAM

Wiring Diagram 30


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Please refer to Operators Manuals for Normal Operation:

290 Operators Manual 78-8078-8977-5 Literature Code 70-2009-4089-1

290G Operators Manual 78-8083-0136-6 Literature Code 70-2009-4687-2

In the event of an error code, unplug the Attest 290/290G Auto-reader and plug it back in to initiate the

self-diagnostic test. If error code re-occurs, customer should call for repair or replacement.

The display panel will indicate malfunctions detected by a specific error code. Refer to the following:

Code Problem Solution

C1 Unit Warm-up Caution Allow a 30 minute warm-up after

Incubation block not at temperature Attest 290/290G Auto-reader is plugged in.

RRBI readings are disabled untilThe incubator block reaches:

290 60C +/-2C

290G 37C +/-2C

C2 RRBI has been removed before incubation Replace RRBI in proper well within 10seconds.completed. All three lights for affected RRBI

will light up and alarm will sound if active.

E1 Temperature Control Error Check heater blanket connection

This error will occur if system is no

longer able to control the temperature ofthe incubator block.

290 60C +/-2C

290G 37C +/-2C

E3 Memory Error. Replace NMOM PCB

E4 Analog-Digital Conversion Error. Replace Sled PCB

E7 Communications Error Check ribbon cable connection between

This error will occur if the internal NMOM and Sled PCB. In addition, check for

communications have failed. power interruptions which could cause

communications error, E7

E8 Bulb Error Check for NMOM Software V1.2

This error occurs if the system detects Replace Sled PCB / old bulb

that the bulb is too dim to properly readRRBI results.

E9 Transport Error Check for loose set screw on sledThis error will occur if the system detects Check for loose set screw on motor drive pulley

a motor failure or positioning problem. Check for loose drive beltCheck bearings for smooth operation

Check for power interruptions which couldcause communications error which could

cause E9


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Trouble Shooting Guide (Continued)

Other Problem Solution

Red, yellow, or green lights do not Specific light burned out replace Display PCBilluminate when expected such as Check ribbon cable connection between

following insertion of a RRBI or when NMOM and Display PCB

time remaining button is pressed.

LED flash test does not function Check alignment and connection of

lid switch

Unwanted alarm sounds when Turn alarm off, refer to features

positive detected. Section.

All three lights blink and buzzer sounds. Replace RRBI in incubation/reader

RRBI has been removed from well within 10 seconds to preventincubation/reader well prematurely. loss of data collected.

Negative Control Vial

Vial not crushed. Crush vial before inserting into well.

Media did not wet the spore strip. Tap vial on table until media wets spore strip.

Control vial was sterilized. Use a non-sterilized vial as a control.

Check chemical indicator on label.

Unexplained positive

Adhesive residue on vial. Do not place tape or labels on vial.

Test vial is absorbing fluorescent Place the vial so it does not come inresidue from a chemical indicator or direct contact with chemical

tape. indicators or tape.

RRBI placed into a well immediately Allow time for previous BI result to clear.

following removal of previous BI.

Inconsistent Read Time

RRBI not inserted all the way. Ensure RRBI is inserted all the way into the

reader well.

RRBI placed into a well immediately Allow time for previous BI result to clear.

following the removal of previous BI.

Intermittent Power Connection Spread split pin on NMOM power jack toensure snug fit with plug.


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1.0 DESCRIPTION: Attest Auto-reader Final Acceptance Specification and Packout

1.1 This procedure covers the following Auto-readers:

Finished Goods # Description

1.1.1 70-2007-2980-7 290 Attest Auto-reader 100VAC


1.1.3 70-2007-2982-3 290 Attest Auto-reader 230VAC1.1.4 70-2007-2983-1 290 Attest Auto-reader 240VAC

1.1.5 70-2007-3170-4 290G Attest Auto-reader 100VAC

1.1.6 70-2007-3171-2 290G Attest Auto-reader 120VAC1.1.7 70-2007-3172-0 290G Attest Auto-reader 230VAC




1.1.11 70-2007-4571-2 293 Attest Auto-reader 230VAC1.1.12 70-2007-4573-8 293 Attest Auto-reader 240VAC

1.1.13 70-2007-4568-8 293G Attest Auto-reader 100VAC1.1.14 70-2007-4570-4 293G Attest Auto-reader 120VAC1.1.15 70-2007-4572-0 293G Attest Auto-reader 230VAC


2.0 SPECIAL INSTRUCTIONS:

2.1 SAFETY PRECAUTIONS: Follow facility general safety guidelines for electronicassembly and testing.

2.2 Standards and specifications given on assembly and component

drawings must be met.

2.3 Hardware fasteners must be fully seated and tight.

2.4 Exterior surfaces must be free from scratches, sharp edges and burrs. Any paint touch-ups

must be neat and blend well with surrounding surface.

2.5 Wiring should be held neatly in place. Wiring should not rub against sharp edges such assheet metal ends or screw threads.

2.6 Switches must operate without binding.

2.7 The person performing assembly operations is responsible for meeting the print

requirements. The checksheet is to be filled out by the assembler for each machine.

2.8 The location of the labels is defined on the prints. The labels are to be mounted straight,without bubbles or lifted edges.

2.9 If a machine fails a test, indicate on the checklist the failure and repair. The unit must betested again after the repair for that function. The results of the test after the repair must

be recorded.

2.10 Any/all unexpected error codes displayed during testing must be resolved before

continuing.

ACCEPTANCE TEST PROCEDURE


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3.0 ASSEMBLY/TEST PROCEDURE:

3.1 In-Process tests

3.1.1 General Workmanship

3.1.1.1 Screw tightness, solder joint quality, wire connections etc

3.1.2 Ribbon Cable Assembly

3.1.2.1 Ensure ribbon cables are inserted fully and routed and secured properly.

3.1.3 Heater Blanket Application

3.1.3.1 Clean outside surface of the incubator block with isopropyl alcoholprior to attaching the blanket.

3.1.3.2 The blanket must be laid flat with no lifting of the blanket away from

the incubator block. (Inspection to be done prior to placing insulation

on unit.)

3.1.4 Home Sensor Adjustment

3.1.4.1 Remove outer case.3.1.4.2 Measure and mark the center of the aluminum optics housing with a

pencil or felt-tip pen.

3.1.4.3 Apply power to unit and allow sled to move to home position.3.1.4.4 View from top of incubator block and ensure that the center of the

optical housing is directly in line with the center of Well #12.

3.1.4.5 If optics is not centered, adjust position of home sensor vane asnecessary and cycle power to re-home sled position.

3.1.5 Sled Spacing Across Incubator Block

3.1.5.1 Unplug motor from NMOM board and slide sled to home position.

3.1.5.2 Adjust spacing between sled optics and edge of incubator block to be0.040 (+ 0.00, - 0.025). Tighten screw to secure incubator block to

frame.

3.1.5.3 Slide sled in front of well #1. Adjust spacing between sled optics andedge of incubator block to be 0.040 (+ 0.00, - 0.025). Tighten screw

to secure incubator block to frame.

3.1.5.4 Slide sled slowly back to home position, ensuring that optics do nottouch incubator block at any point along the full range of travel. Athome position, verify spacing between sled optics and edge of

incubator block to be 0.040 (+ 0.00, - 0.025). If not within

specification, go to 7.2.4.2 and repeat process.

3.1.5.5 Plug motor cable back in to connector.


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STEPS TO REMOVE COVER OF 293/293G AUTO-READER:

1. Remove 4 screws from back cover

2. Remove 8 screws used to mount the top cover


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3. Lift RIGHT side of top cover. Display Ribbon cables are connected on LEFT side of top cover.4. Un-plug or Re-plug Display Ribbon cables as needed.

RIBBON CABLES

ROTARY SWITCHES

LIFT COVER


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5. Set rotary switches for all 3 tiers to setting 0 for functional testing.6. Unplug USB-to-RS232 adapter at the RS232 end and connect computer serial cable for functional

testing of each tier (top, center, bottom).

7. After testing:- Re-plug USB-toRS232 adapters for each tier- Re-set all three rotary switches based on labeling (1292 or 1294)

Ensure all Display Ribbon Cables are securely connected

Proper Repairs Prior to Shipping the 293/293G

293/293G Frame Top ViewDrill holes on ends as shown for mounting stand-offs.

USB to RS232 ADAPTER


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Proper Repairs Prior to Shipping the 293/293G Continued:

Auto-reader Display Ribbon Cable Disconnection Resolution.Attach from ribbon cable to ribbon cable connector.

Proper Repairs Prior to Shipping the 293/293G includes:

Add Dual Locks, Stand-offs, lock nuts and screws as shown below. Reference 293/293Gassembly drawing for full blow-up of stand-offs, etc.


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ITEM PART DESCRIPTION QUANTITYNUM. NUMBER REQUIRED

1 78-8083-1005-2 3M 8422 Polyester Ribbon Connector Tape 3

2 78-8083-1004-5 Alignment pins, for shipping 4

9 26-1017-1620-2 Screw, 8-32 x 0.5, Pan, Phil, Blk Zn 639 70-0705-3010-3 Dual Lock SJ3263 Round 13/16 640 70-0707-4015-7 Dual Lock SJ3550 Square 1 by 1 6

41 26-1017-6006-9 Standoff, 8-32, M/F Hex, 1.25 6

42 26-1017-3845-5 8-32 KEPS Lock-nuts, Zinc, Hex 61

For Shipping 293/293G:

Note: Alignment Pins to be placed in well #1 and #12 for each heater block.

4.0 ACCEPTANCE TEST PROCEDURE:

Note: This procedure to be done for 100% of units. For 293/293G Auto-readers, perform each test for each

of the three tiers of incubators (top, center, bottom).

4.1 Error Code Test:

4.1.1 Disconnect power and remove cover.

4.1.2 Set rotary switch to position 1.

4.1.3 Generate a failure condition by one of the following 2 methods:4.1.3.1. Disconnect heater blanket.

4.1.3.2. Disconnect motor wire connector and move sled off home position

toward Well #1.

4.1.4 Apply power to the unit and verify the error code:

4.1.4.1. "E1" for temperature failure.4.1.4.2. "E9" for motor movement failure.

4.1.5 Disconnect power, repair failure, apply power, and verify no error code is

displayed.

4.2 Display Verification:


4.2.2 Apply power to the unit.

4.2.3 Verify 7-segment display shows "8.8." on initial power-up as a LED check.

4.2.4 Verify 7-segment display shows "F1" (1291 BIs) as a set-up mode indication.4.2.5 7-segment display will show "C1", the warm-up caution code, if not up to

temperature.

4.2.6 Open cover on unit and ensure all red, yellow, and green LEDs light.4.2.7 Insert a BI into any well of the test unit.

4.2.8 Press "time remaining" button and verify that "1H" is shown for the BI.4.2.9 Wait for the unit to heat up and the first reading to be taken.

4.2.10 Remove the BI and verify that "C2" is displayed and the audible signal sounds.

4.2.11 Re-insert BI within 10 seconds and verify that caution code clears and audiblesignal ends.

4.2.12 Press both buttons and verify that period is displayed on the 7-segment display

to signify the audible signal is disabled.

293/293G ASSEMBLY


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4.2.13 Remove the BI and verify that "C2" is displayed and the audible signal does notsound.

4.2.14 Re-insert BI within 10 seconds and verify that caution code clears.4.2.15 For 293/293G Auto-reader, press both buttons for EACH tier and verify that

period is toggled on/off on the 7-segment display of ALL THREE TIERS

SIMULTANEOUSLY.

4.2.16 Remove BIs.4.2.17 Remove Power.

4.3 Automated Test System Setup, Manufacturing Test System (MTS)


4.3.2 Plug serial cable into connector on unit.4.3.3 Cycle power to unit and start Automated Test System Software or select File,

New if test system is already running.

4.3.4 Enter serial number of unit being tested.4.3.5 Perform the following tests:

Note: If any tests fail, re-test once before re-working unit.

4.4 Serial Communications

4.4.1 Select "Serial Communication" test and press "Test"4.4.2 Verify Successful Result in status display box.

4.5 Lid Test (290/290G Only)

4.5.1 Select "Lid Test" test and press "Test" or press "Next Test".

4.5.2 Open lid and leave open when prompted and press "OK".

4.5.3 Close lid when prompted and press "OK".

4.5.4 Verify Successful Result in status display box.

4.6 BI Hole Test

4.6.1 Select "BI Hole Test" test and press "Test" or press "Next Test".

4.6.2 Make sure all wells are empty when prompted, close lid, and press "OK".4.6.3 Insert BIs into all wells when prompted, close lid, and press "OK".


4.6.5 Remove all BIs from wells.

4.7 Config Switch Test

4.7.1 Select "Config Switch" test and press "Test" or press "Next Test".4.7.2 Verify Successful Result in status display box.

4.8 Home Sensor Test

4.8.1 Select "Home Sensor" test and press "Test" or press "Next Test".


4.9 E2ROM Read/Write Test

4.9.1 Select " E2ROM Read/Write" test and press "Test" or press "Next Test".


4.10 Servo Reading Test

Note: This test uses "Fluorescent Standards, Model 290", part number 78-8078-8421-4


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4.10.1 Select "Servo Reading" test and press "Test" or press "Next Test".4.10.2 Insert Low Standard into Well #12 when prompted, close lid, and Press "OK".4.10.3 Insert High Standard into Well #12 when prompted, close lid, and Press "OK".4.10.4 Verify completion of test in status display box.4.10.5 Remove fluorescent standard from unit.

4.11 Motor Movement Test

4.11.1 Select "Motor Movement" test and press "Test" or press "Next Test".


4.12 Motor Slip Test

4.12.1 Select "Motor Slip" test and press "Test" or press "Next Test".


4.13 Fluorescent Standards Verification

4.13.1 Press "View Report" icon on toolbar.

4.13.2 Verify Low Fluorescent Standard Std Dev is less than 2.4.13.3 Verify High Fluorescent Standard Std Dev is less than 4.4.13.4 Verify High Fluorescent Standard Average is less than 250.

4.13.5 Verify difference between High and Low averages is greater than 110.4.13.6 Close Report.

5.0 CALIBRATION PROCEDURE:

5.1 Temperature Set Point Verification

Use a traceable temperature measurement system and appropriate temperature probe that

makes good contact with incubator block walls to verify temperature in Wells #6 and #12

are within the range including:

Model 290/293 59.0C 61.0C

Model 290G/293G 36.0C 38.0CMeasure temperatures for an appropriate amount of time after temperature has stabilized

to ensure temperature remains within specification.NOTE: Recommended measurement system or, similar: Fluke 77 Multi-meter andFluke 80K Thermocouple Module.

Temperature probe assembly, 3M stock number 78-8078-8982-5

5.2 Fluorescent Standards Verification

Fluorescent standards (78-8078-8421-4) intensity should be verified every 2year (24

months)according to the procedure outlined on "Calibration Procedure for Fluorescent

Standards", drawing number 78-8078-9589-7.

6.0 PRESHIPMENT CHECKS/PACKOUT PROCEDURE:

6.1 Filled out and signed Machine Check-sheet

6.2 Labeling

All labeling (serial plate, warning, instructions etc.) to be legible. All labeling to be installed

per print requirements. (Adhered to unit or placed inside of box etc.)

6.3 Preparation for Shipping


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6.3.1 Place rotary switch to appropriate position based on the BI label present on theunit according to the following table. Apply Power and verify the correct setup

mode is displayed on 7-segment display according to the following table.

Setup Mode Rotary Switch

Position

7-Segment Power-up

Display

1291 BIs, Blue Label 1 F1

1292 BIs, Brown Label 3 F31291/1292 BIs, Blue/Brown Label 7 F7

1294 BIs, Green Label d Fd

6.3.2 With unit unplugged, insert a small flat blade screwdriver tip (Apex 00 orequivalent) into the center split pin of the power jack on the NMOM PCB.

Spreading this split pin will ensure a snug fit of the power plug upon receipt by

customer.

6.3.3 Clean the machine of all smudges, fingerprints or water.


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In process test

characteristics

Test Results

pass/fail

1) General

Workmanship

2) Ribbon Cable

3) Heater Blanket

4) Home Sensor

Adjustment

5) Sled Spacing

Across Bock

6) Error Code Test

7) Display Verification

Approvals:

_______________________ _______

Service Date

290/290G Acceptance Check Sheet page 1 of 2

Serial Number _______________________

Model Info (Check Appropriate)


70-2007-2980-7 290 Attest Auto-reader 100VAC




70-2007-3170-4 290G Attest Auto-reader 100VAC





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In process test

characteristics

Test Results

pass/fail

8) FluorescentStandards

Low Standard StdDev


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In process test

characteristics

Test Results pass/fail

TOP TIER


CENTER TIER


BOTTOM TIER

1) General

Workmanship

2) Ribbon Cable

3) Heater Blanket

4) Home Sensor

Adjustment

5) Sled Spacing

Across Bock

6) Error Code Test

7) Display Verification

Approvals:

_______________________ _______

Service Date

293/293G Acceptance Check Sheet page 1 of 2

Serial Number _______________________

Model Info (Check Appropriate)








70-2007-4572-0 293G Attest Auto-reader 230VAC70-2007-4574-6 293G Attest Auto-reader 240VAC


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In process test

characteristics


TOP TIER


CENTER TIER


BOTTOM TIER

8) FluorescentStandards

Low Standard StdDev


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Illustrated Parts List


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ITEM PART DESCRIPTION QUANTITY

NUM. NUMBER REQUIRED

1 78-8083-0098-8 MEMBRANE PANEL OVERLAY 1

2 78-8078-8675-5 DISPLAY ASSY 13 26-1002-5214-2 SCREW, MACH,6-32 X.250, PAN, PHIL 12

4 78-8083-0207-5 ENCLOSURE ASSY 15 78-8078-8668-0 N/A FRAME ASSY 1

7 78-8083-0086-3 N/A BOTTOM PLATE 1

8 26-1004-2652-2 WASHER, FLAT #6 39 70-0700-7979-6 BUMPER,3M 6

10 26-1002-5195-3 SCR 2-56 X .188, MACH PAN ZN 2

11 78-8078-8756-3 LID SWITCH ASSY 1

12 26-1000-4179-2 N/A NUT, MACH, 2-56, ZN 213 78-8083-0195-2 N/A LID SENSOR BRACKET 1

14 78-8078-0402-2 N/A LABEL-SERVICE CENTER 115 78-8083-0087-7 COVER, BACK 1

16 26-1012-1254-1 N/A CLAMP,RICHO, CFCC-3-05A-RT 1

16A 26-1014-7689-8 N/A CLIP for FLAT CORD, WHITE 1

16B 26-1017-3696-0 CLIP for ROUNDED CORD 178-8078-8764-3 N/A DECAL, CAUTION 1

17 78-8078-8773-8 N/A SERIAL PLATE, AUTO READER 1

290/290G ASSEMBLY


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18 78-8078-8878-5 N/A LABEL, SHOCK/EXCLAMATION 119 26-1004-3301-5 SCREW, 2-56 X .125 2

20 26-1017-0657-5 SCREW, 6-19 X .250, PAN, PHIL 121 26-1014-7284-8 CABLE, ELEK PARALEX 1.00 MM 1

22 78-8078-9170-6 CARD,INSERT 1

23 26-1014-7450-5 ENVELOPE, CLEAR, 2.5 X 4.0 124 26-1012-1254-1 N/A CLAMP,RICHO, CFCC-3-05A-RT 1

26 26-1017-2681-3 N/A SCREW, MACH, 4-40 X .188, PHIL 1

N/A INDICATES PART NOT AVAILABLE

CONNECTIVITY KIT, Auto-reader, New Style: Connectivity Kit is necessary to link

the 290 Auto-readers to a PC with Merlin SW (Microsystems SPM).

1 78-8083-1166-2 Connectivity Kit2 78-8083-1167-0 Cover-plate

3 78-8083-1211-6 Instruction Sheet

290/290G ASSEMBLY


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1 78-8078-8772-0 N/A MEMBRANE PANEL OVERLAY 12 78-8078-8675-5 DISPLAY ASSY 1

3 26-1002-5214-2 SCREW, MACH,6-32 X.250, PAN, PHIL 5

4 78-8078-8869-4 N/A MASK,DISPLAY BOARD 25 78-8078-8759-7 N/A ENCLOSURE 1

6 78-8078-8668-0 N/A FRAME ASSY 1

7 78-8078-8761-3 N/A BOTTOM PLATE 18 26-1002-5216-7 SCREW, MACH, 6-32 X .375, PAN PHIL 12

9 70-0700-7979-6 BUMPER,3M 6

10 26-1002-5206-8 SCR 4-40 X .375, MACH PAN ZNC 12

11 78-8078-8756-3 LID SWITCH ASSY 1

12 26-1003-4052-5 N/A NUT, MACH, 4-40, KEPS 413 78-8078-8760-5 N/A LID SENSOR BRACKET 1

14 78-8078-0402-2 N/A LABEL-SERVICE CENTER 115 78-8078-8880-1 COVER, BACK 1

16 78-8078-8764-3 N/A DECAL, CAUTION 1

18 78-8078-8773-8 N/A SERIAL PLATE, AUTO READER 119 78-8078-8878-5 N/A LABEL, SHOCK/EXCLAMATION 1

290/290G ASSEMBLY (OLD STYLE CASE)


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20 26-1002-5204-3 SCREW, MACH, 4-40 X .250, PAN, PHIL 4

21 26-1014-7284-8 CABLE, ELEK PARALEX 1.00 MM 122 78-8078-9170-6 CARD,INSERT 123 26-1014-7450-5 ENVELOPE, CLEAR, 2.5 X 4.0 1

24 26-1014-7495-0 N/A WASHER,ASM CO., 2

25 26-1012-1254-1 N/A CLAMP,RICHO, CFCC-3-05A-RT 126 78-8125-7843-9 BI ID LABEL KIT 1291, 1292, 1

& 1291/1292 (NOT SHOWN)

27 78-8078-8978-3 BI ID LABEL 1294 (NOT SHOWN) 1


CONNECTIVITY KIT, Auto-reader, Old Style: Connectivity Kit is necessary to link the290 Auto-readers to a PC with Merlin SW (Microsystems SPM).

1 78-8083-1168-8 Connectivity Kit

2 78-8083-1169-6 Cover-plate

3 78-8083-1211-6 Instruction Sheet

290/290G ASSEMBLY (OLD STYLE CASE)


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1 78-8078-8668-0 N/A FRAME ASSY 32 78-8083-0380-0 LABELS, BI WELLS 1294 3

2A 78-8083-0381-8 LABELS, BI WELLS 1292 33 78-8078-8772-0 MEMBRANE PANEL OVERLAY 3

4 26-1003-6373-3 SCREW, MACH,6-32 X.375, PAN, PHIL 12

6 78-8083-0306-5 END CAPS, PAIR 1

7 78-8083-0307-3 PLATE, BACK 18 26-1002-5226-6 SCREW,MACH,8-32 X.375, PAN, PHIL 10

9 26-1017-1620-2 SCREW,MACH,8-32 X.5,PAN, PHIL, BLK 14

10 78-8083-0303-2 CASE 111 78-8083-0396-6 DECAL, FRONT 1

12 78-8083-0305-7 BRACKETS, PAIR 113 78-8083-0304-0 BASE PLATE 114 78-8083-0392-5 POWER CABLE ASSY 1

15 70-0700-7979-6 BUMPER,3M 6

19 78-8083-0395-8 CONVERTER, SERIAL TO USB 3

20 78-8083-0394-1 USB HUB; 121 26-1007-4847-9 TAPE, DBL COATED, 1W 3M VHB AR22 78-8083-0391-7 CABLE, FLAT, RS232 3

293/293G ASSEMBLY


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3M2009 78-8078-9168-0 Page 27 of 38



23 78-8078-8869-4 N/A MASK,DISPLAY BOARD 624 26-1002-5214-2 SCREW, MACH, 6-32 X .25, PAN PHIL 12

25 78-8078-8675-5 DISPLAY ASSY 3

26 78-8083-0418-8 SPACER, THERMAL INSUL 327 26-1017-6304-8 CABLE, ELEK PARALEX 1.00 MM 3

29 78-8083-0437-8 WIRE HARNESS, DISPLAY 1

30 26-1017-2068-3 USB PANEL MOUNT JACK 131 26-1017-2787-8 DUST COVER, USB 1

32 26-1002-5204-3 SCREW, MACH, 4-40 X .25, PAN PHIL 2

33 26-1003-4052-5 NUT, 4-40, KEPS 2

34 26-1009-7475-2 TIE WRAP, #10 MT HOLE 1


293/293G ASSEMBLY


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Page 28 of 38 3M2009 78-8078-9168-0

ITEM PART DESCRIPTION QUANTITYNUM. NUMBER REQUIRED

1 78-8078-8389-3 NMOM ASSYY 1

2 26-1002-5218-3 SCREW, MACH, 6-32 X .500, PAN, PHIL 53 26-1014-7498-4 N/A SPACER,ASM CO, 3031R-F-.275,.250 3

4 26-1002-5216-7 SCREW,MACH,6-32 X .375, PAN, PHIL 2

5 78-8078-8386-0 N/A FRAME 16 26-1011-8385-8 SCR, M3 X .5 X 5.0,SKT,HEX SKT 4

7 78-8078-8758-9 N/A SLED LOCATOR 1

8 26-1002-5204-3 SCREW,MACH,4-40 X .250,PAN, PHIL, 29 26-100508084-9 N/A NUT, MACH,4-40, KEPS 4

10 78-8078-8717-5 STEPPER MOTOR 1

11 26-1004-7298-8 N/A PULLEY, BERG MFG, TP7A5MW2-15, 112 26-1014-7496-6 N/A WASHER, ASM CO., 99B-.062-D, FLAT, 213 78-8078-8725-8 N/A SPACER, INCUBATOR BLOCK 1

14 78-8078-8766-2 INCUBATOR BLOCK ASSY 1

15 26-1014-7300-2 N/A SPACER,ASM CO., 3031R-D-.312, .250 216 78-8078-8667-2 N/A SLED HOLDER ASSY 1

FRAME ASSEMBLY


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3M2009 78-8078-9168-0 Page 29 of 38



17 78-8078-8714-2 N/A IDLER PULLY SPRIG 118 78-8078-8716-7 N/A IDLER PULLEY 1

19 26-1014-7125-3 N/A RETAINER RING, WALDES TRUARC, 2

20 78-8078-8715-9 N/A SHAFT, IDLER PULLY 121 78-8656-4008-6 N/A RETAINING RING, TRUARC,5133-18-H 422 78-8078-8703-5 N/A GUIDE ROD 2

23 26-1014-7301-0 BELT, TIMING, B.C. OF A.,MXL270T80 1

24 78-8078-8905-6 N/A PLATE, CAP 1


290 FRAME ASSEMBLY


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Page 30 of 38 3M2009 78-8078-9168-0



1 78-8078-8211-9 N/A SLED ASSEMBLY 12 78-8000-7655-2 SCR, 2-56 X 1.0 1

3 26-1014-7302-8 N/A O-RING 2

4 78-8078-8670-6 N/A SIDE COVER, LEFT 15 78-8078-8671-4 N/A GLASS EXCITATION FILTER 2

6 78-8078-8674-8 N/A MASK 17 78-8078-8312-5 N/A REFLECTOR 18 78-8078-8669-8 N/A SIDE COVER, RIGHT 1

9 26-1000-4179-2 N/A NUT, 2-56 X 1

10 78-8078-8673-0 N/A GLASS EMISSION FILTER 111 78-8078-8672-2 N/A GELATIN EMISSION FILTER 1

12 26-1017-2781-1 LAMP 1

12A 26-1014-7272-3 LAMP, ALTERNATE 0

13 26-1004-3301-5 SCR.2-56 X .125 2


SLED ASSEMBLY


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3M2009 78-8078-9168-0 Page 31 of 38



1 78-8078-8704-3 N/A GUIDE PLATE 1

2 26-1014-7294-7 N/A BEARING, OILITE,AA-309-4 4

3 26-1014-7295-4 N/A SPACER, ASM CO., 14030-H-A-.875-3 24 26-1014-9893-4 CABLE, FLEX 1

5 78-8078-8707-6 N/A BELT TENSIONER PIN 1

6 78-8078-8706-8 N/A BELT LOCKING PIN 17 26-1011-5909-8 SCR, SET, 2-56 X .125 1

8 78-8078-8705-0 N/A PCB CLAMP 1

9 78-8078-8710-0 N/A STRAIN RELIEF 110 78-8078-8666-4 SLED ASSEMBLY 1

11 26-1002-5206-8 SCR, 4-40 X 3/8 MACH PAN PHIL ZNC 4

12 26-1014-7293-9 SCR, SET, 4-40 X .375 1

13 26-1014-7296-2 N/A SPACER, ASM CO., 14030-H-A-1.125-3 2


SLED HOLDER ASSEMBLY


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Page 32 of 38 3M2009 78-8078-9168-0

3M Attest Model 290/290G Theory of Operation

Theory of Operation

This section will describe mechanical, electrical and software component aspects of the 3M Attest

290/290G Auto-reader/Incubator.

A. Electro-Optics Apparatus

The sled board contains the electro-optics apparatus for the 3M Attest 290/290G Auto-reader. Insidethe enclosed extrusion are two photo sensors and a bulb, or excitation source. Light from the excitation

source is shown on and reflected off the Biological Indicator, filtered to the desired wavelength, and a

proprietary embedded control system accurately measures the fluorescent activity. The embedded

control system also monitors the bulb intensity. This apparatus should not be disassembled unless themanufacturing guidelines are followed.

B. Sweep Measurement Timing

The measurements of relative fluorescence for each biological indicator are internally timed by amicro-controller to take place at the same on time for all readings. The fluorescent measurement

time and the amount of time to transport the elecro-optics to another biological indicator well is 2

seconds. See Table 1 below.

Reader/

incubator Well #

Order

Read

Time after bulb turns

on to fluorescencemeasured

12 1st T011 2nd T0+2

10 3rd T0+4

9 4th T0+6

8 5th T0+8

7 6th T0+10

6 7th T0+12

5 8th T0+14

4 9th T0+163 10

th T0+18

2 11th T0+20

1 12th T0+22

Table 1

Since the excitation sources output intensity varies with respect to time on and the proprietaryembedded control systems ability to minimize the output variations, this timing technique assures that

each biological indicator placed into a reader incubator well will be read in the same excitation source

on time with a variation of less than +/- 1% of the total specified readout time.

C. Measuring Fluorescent Intensity Increases.

Design considerations for the Electro-Optics apparatus were made on the assumption that the intensityincrease of fluorescence would be measured. Since background fluorescence can vary between BI

vials, this measurement of fluorescent intensity increase eliminates the requirement for BIs to have

similar background fluorescent measurements. Furthermore, since the detection of positive andnegative BIs will be based on the change in fluorescence during incubation instead of fixed threshold

values, this measurement method eliminates the need for daily calibration.

D. Excitation Intensity

Each time a biological indicator is read by the electro-optics apparatus, the excitation source will

remain on for a fixed length of time, regardless of the number or location of the biological indicators.


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3M2009 78-8078-9168-0 Page 33 of 38

After this on time, the excitation source intensity will be measured. This technique will allow theability to measure the output intensity of the excitation source after it has been operated, and stabilized.

If this output intensity is less than a chosen set-point, then the excitation source failure determination

can be ascertained. Error message (E8) will be displayed for the user if the excitation source is too dimto accurately measure fluorescent intensity.

E. Sled Positioning.

The Electro-Optics Apparatus is mounted on a moveable platform called a Sled. The Sled position isreferenced to a home position via a sensor mounted on the sled and a vane mounted on the frame. This

establishes the home position of the sled. The home position is determined automatically at power-up

as well as when a series of BI readings are completed. Positioning is accomplished with a stepper

motor as an open loop system. The motor is given a certain number of steps to move the Sled from

one well position to the next. The number of steps moved away from the home position isaccumulated, and when the Sled returns home, the number of steps needed to move home is subtracted

from the accumulated value. A difference of more than 3 steps between the two values 3 consecutive

times will result in the system preventing normal operation, and an error message (E9) will bedisplayed for the user. If the difference is more than 3 steps, the readings will be ignored and retaken.

F. BI Insertion Detection.

BI presence in each Incubator Block Well is determined using a photoelectric sensor mounted on theNMOM board and placed next to each well. When the unit detects the cover open, the BI detection

sensors are read and the A/D value is compared to a setpoint. A value above the setpoint indicates the

presence of a BI. A value below the setpoint indicates an empty well.

G. Incubator Block Temperature Control.

Incubator Block temperature is maintained by a heater blanket mounted on the Incubator Block. A

thermistor on the heater blanket is measured with an A/D converter and the value is compared to asetpoint. If the value is at least one count above the setpoint, the heater is turned off. If the value is at

least one count below the setpoint, the heater is turned on. The setpoint is saved in the EEPROM. The

value is automatically changed via the rotary setup switch for 60C or 37C operation. When the unitis first turned on, a warm-up caution code (C1) is displayed and the unit is non-functional until the

setpoint is reached. During normal operation, a high or low temperature condition will result in the

system preventing normal operation, and an error message (E1) will be displayed for the user.

H. Display.

The display consists of red, yellow, and green LEDs for each Incubator Well and dual 7-segmentdisplays. When a BI is inserted into a well, the yellow LED will light, indicating a BI in process.After the full incubation time, if the BI result is negative, the green LED will light and remain on until

the BI is removed. If at any time during incubation a positive result is determined, the red LED will

light and remain on until the BI is removed. The buzzer will also sound if enabled, until the "buzzer"

button is pressed or the BI is removed. If a positive BI is removed then red LED will remain lit forapproximately 10 seconds, allowing the BI to be replaced for growth readout purposes. The 7-segment

display will display caution and error codes as well as time remaining for each BI if the "time

remaining" button is pressed. The LEDs all light in a test mode when the cover is opened. If a BI is

removed before incubation is complete, all LEDs for that well will light and the buzzer will sound for

10 seconds, with caution code C2 displayed, indicating the need to replace the BI. After 10 seconds,the BI data is lost.


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Certificate of Calibration

Serial Number: _____________________

Model Number: ____________________ 290 Auto-reader ____________________ 290G Auto-reader

The serial number listed above has been tested and found to comply with 3Ms manufacturingspecifications applicable to subject device at time of manufacture. It has been tested with NISTtraceable instrumentation to verify it meets the following specifications.

3M Test Procedure Number: C-05-005002

1) Temperature Specification:

290 60C +/-2C290G 37C +/-2C

Well #6 Measurement C

Well #12 Measurement C

2) Relative Fluorescence Measurement Increase Specification:

Greater Than 110 Relative Fluorescence Units

Measurement Units

3) Fluorescence Measurement Standard Deviation Specification:

Less than 4 Relative Fluorescence Units

Measurement Units

Test Equipment:

Equipment Used (Temperature):Equipment ID:Calibration Date:Calibration Due:

Equipment Used (Fluorescence): Fluorescent StandardsEquipment ID: 78-8078-8421-4Calibration Date:Calibration Due:

For factory calibration and re-certification of this equipment contact:

3M Health Care Service Center

Suite 200, Bldg. 5023350 Granada Avenue NorthOakdale, MN 551281-800-292-6298Fax: 1-800-770-8016

Outside of the USA, contact your local 3M Subsidiary.

Calibrated By:_________________________________ Calibration Date: _________________________________

QC: _________________________________ Date: _________________________________


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Certificate of Calibration

Serial Number: _____________________

Model Number: ____________________ 293 Auto-reader ____________________ 293G Auto-reader

The serial number listed above has been tested and found to comply with 3Ms manufacturingspecifications applicable to subject device at time of manufacture. It has been tested with NISTtraceable instrumentation to verify it meets the following specifications.

3M Test Procedure Number: C-05-005002

1) Temperature Specification: 293 60C +/-2C, 293G 37C +/-2C

Well #6 Measurement CWell #12 Measurement CWell #18 Measurement CWell #24 Measurement C

Well #30 Measurement CWell #36 Measurement C

2) Relative Fluorescence Measurement Increase Specification: Greater Than 110 Relative Fluorescence Units

Top Tier Measurement UnitsCenter Tier Measurement UnitsBottom Tier Measurement Units

3) Fluorescence Measurement Standard Deviation Specification: Less than 4 Relative Fluorescence Units

Top Tier Measurement UnitsCenter Tier Measurement UnitsBottom Tier Measurement Units

Test Equipment:

Equipment Used (Temperature): _______________________Equipment ID: _______________________Calibration Date: _______________________Calibration Due: _______________________

Equipment Used (Fluorescence): Fluorescent StandardsEquipment ID: 78-8078-8421-4Calibration Date:Calibration Due:

For factory calibration and re-certification of this equipment contact:

3M Health Care Service CenterSuite 200, Bldg. 5023350 Granada Avenue NorthOakdale, MN 551281-800-292-6298Fax: 1-800-770-8016

Outside of the USA, contact your local 3M Subsidiary.

Calibrated By:_________________________________ Calibration Date: _________________________________

QC: _________________________________ Date: _________________________________


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3M Health Care

3M Center

Building 275-4E-01St. Paul, MN 55144-1000

1-800-228-3957

3M Health Care

D-41453 Neuss, Germany

Recycled paper

40% pre-consumer10% post-consumer

Litho in U.S.A. with 3M film,

proofing systems and offset

plates.

Attest is a registered trademarkof 3M.

3M 200978 8078 9168 0 Rev D

Manual Tecnico 290

Documents

Transcript of Manual Tecnico 290