Making Sense of our Restriction Digests
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Making Sense of our Restriction Digests Or not…
description
Making Sense of our Restriction Digests. Or not…. Theoretical Pvu II digests of plasmids. p. 2B-10. Why are there 3 bands in the uncut lane?. p. 2B-10. What is the size of the insert?. 2.9 Kb. 700 bp - 700 bp = 0 bp. p. 2B-13. Is there a Pvu II site in the insert - PowerPoint PPT Presentation
Transcript of Making Sense of our Restriction Digests
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Making Sense of our Restriction Digests
Or not…
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Theoretical PvuII digests of plasmids
p. 2B-10
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Why are there 3 bands in the uncut lane?
p. 2B-10
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What is the size of the insert?
p. 2B-13
700 bp - 700 bp = 0 bp
2.9 Kb
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Is there a PvuII site in the insertAre there multiple bands?
p. 2B-13
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Do the results from the digest and the PCR agree?
p. 2B-14200 bp - 200 bp = 0 bp
700 bp - 700 bp = 0 bp
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How do we figure out the insert size from the 20JM4.10 digest?
1200 bp + 400 bp-700 bp = 900 bp
2900 bp1200 bp
400 bp
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Section 12 ResDig Gel
Run 9/28
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Section 34 ResDig Gel
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Section 58 Res Digest gel