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  • 8/6/2019 MACS Technology Flyer


    Consistent, high-quality separations

    Easy handling

    Compatible with downstream applications

    Innovative products

    From lab bench to clinical research applications

    MACS TechnologyGold standard in cell separation

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    CliniMACS Plus InstrumentThe CliniMACS Systema closed and sterilesystem for separation of large cell samples

    From lab bench to clinical research applications

    From small samples to samples ranging up to 1011 cells by

    using columns with dierent cell loading capacity and their

    suitable separators

    Automated cell separation: The autoMACS Pro Separator

    allows standardization o requent cell separations.

    Sterile separations in a closed system: The CliniMACSPlus

    Instrument in combination with GMP reagents opens new

    opportunities or clinical research.

    CD133+ cellsisolated withMACS Technology becameadherent and CD133during cultivation butgave rise to non-adherentCD133+ cells buddingrom the adherent cellsurace by asymmetriccell division

    Compatible with downstream applications

    Gentle on cells: small MicroBeads in combination

    with MACS Column Technology allow the purication

    o viable and unctionally active cells.

    No inuence on analysis: small MicroBeads do not inuence

    ow cytometric or microscopic analysis.

    Suitable or cell culture and in vivo experiments: MicroBeads

    are biodegradable, the short procedure and gentle

    column technology avoid mechanical stress.

    Molecular biology experiments: limited amount o small

    MicroBeads avoid any inuence on urther experiments.

    Innovative products

    Continuous product development with ocus on current

    research and clinical research needs

    T cells: isolation o regulatory T cells, naive and memory/eectorcells, Th2 cells, or even antigen-specic T cells according to their

    secretion o cytokines

    Stem cells: CD133the marker or isolation o hematopoietic

    and nonhematopoietic stem cells

    Dendritic cells: unique kits or isolation o myeloid and

    plasmacytoid human dendritic cells and mouse dendritic

    cell subsets

    Spleen cells beore separation Isolated CD4+CD25+ T cells

    Plasmacytoid blooddendritic cellsisolated with MACSTechnologyandstained

    with May-Grnwald-Giemsa

    CD4+CD25+ regulatory T cells isolated rom mouse spleen usingMACS Technology

    autoMACS Pro SeparatorWalk-away cell sorting of multiple samples

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    MACS TechnologyGold standard in cell separation

    Consistent, high quality separations

    Optimal purity and recovery due to highly specic reagents

    combined with efcient MACS Column Technology

    Frequent cells such as T, B, NK cells and monocytes,

    but also rare cells like stem cells, antigen-specic T cells,

    and dendritic cell subsets

    For any cell type: over 200 reagents or the direct isolation

    o human, mouse, rat, and non-human primate cells;

    any other cell type can be isolated using indirect labeling

    with MicroBeads

    Reliable and consistent results: based on extensive

    experience in cell separation systems and rigorous

    quality control

    Easy handling

    Convenient procedure:

    easy-to-use system, applicable in every lab

    Fast results: cells can be puried in up to 30 minutes

    or positive selection or depletion due to short incubation

    time and efcient MACS Column Technology;

    no time-consuming bead detachment necessary

    For positive selection and depletion strategies:

    both labeled and unlabeled ractions can be obtained

    with excellent purity and recovery

    Prepackaged starting kits available:

    consisting o a magnet and stand, columns and reagents

    CD4+ T cells isolated using an LSColumn and a MidiMACS Separator

    CD4+ T cells isolated usingthe autoMACS Separator

    Bone marrow cellsbeore separation Isolated CD34+ cells

    Mouse spleen

    Human bone marrowMiniMACS SeparatorBasic tool for the lab:MiniMACS Separator with an MS Column

    QuadroMACS SeparatorFor the simultaneous separation of 4 samples

    The advantages

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    Magnetic separation

    MACS Column Technologyprovides a high-gradient

    magnetic ield.





    Untouched isolation by

    depletion o speciic cells

    Magnetic labeling

    Gentle to cells;

    minimal inluence

    on downstream


    Elution of the labeled cell fraction

    Optimal resultseven or rare cells

    by using positive selection

    N S

    Electron micrographs on this page with courtesy o Pro. Peter Groscurth, Institute o Anatomy, University o Zrich, Switzerland.

    MACS TechnologyGold standard in cell separation

    MACS Technology

    MACS Technology has become the standard

    method or cell separation. Numerous

    publications have proven its versatility ormultiple applications: cell separations with

    consistent high-quality results rom lab bench

    to clinical applications, rom small to large

    scale, rom requently occurring cells to rare

    cells and sophisticated subsets. Miltenyi Biotec

    provides researchers worldwide with the tools

    or high-quality separations.

    The principle

    MACS Technology is based on MACS

    MicroBeads, MACS Separators, and

    MACS Columns. MACS MicroBeads aresuperparamagnetic particles o approximately

    50 nanometers in diameter. They are

    composed o a biodegradable matrix, and it is

    thereore not necessary to remove them rom

    cells ater the separation process.

    Usually, MACS MicroBeads do not alter

    structure, unction, or activity status o labeled

    cells and are not known to interere with

    subsequent experiments.

    MACS Technology takes place within MACS

    Columns. When a MACS Column is placed in a

    MACS Separator, a strong permanent magnet,

    a high-gradient magnetic eld is induced on

    the column matrixstrong enough to retain

    cells labeled with minimal amounts o MACS

    MicroBeads. Unlabeled cells pass through and

    can be collected; labeled cells are released

    ater removal o the column rom the magnet.

    Thus, with MACS Technology both labeled and

    unlabeled cell ractions can easily be isolated

    with high purity. The entire procedure o

    positive selection or depletion takes less than

    30 minutes, and cells can immediately be used

    or urther experiments.

    MACS Cell Separation is based on the use o MACS

    MicroBeads, MACS Columns, and MACS Separators.

    Cells can easily be purifed or depleted in less than30 minutes.






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    Direct labeling is the astest way o magnetic labeling. It

    requires only one labeling step since the specic antibody

    is directly coupled to the magnetic particle. Direct labelingreduces the number o washing steps and, thereore, avoids

    unnecessary cell loss. Miltenyi Biotec provides highly specic

    monoclonal antibodies or low background and optimal

    separation, targeted against surace markers o cells rom

    various species such as human, mouse, rat, and non-human

    primates. Fluorochrome-conjugated MACS Antibodies can be

    added or the purpose o analyzing magnetically separated cell

    ractions by ow cytometry or uorescence microscopy.

    Direct magnetic cell labeling Indirect magnetic cell labeling

    Indirect labeling is perormed i no direct MicroBeads or

    the cell type o interest are available. Almost any monoclonal

    or polyclonal antibody targeting any cell type rom anyspecies can be used or indirect labeling. Cells are labeled

    with a primary antibody that is unconjugated, biotinylated, or

    uorochrome-conjugated. In a second step, magnetic labeling

    is perormed by using Anti-Immunoglobulin, Anti-Biotin,

    Streptavidin, or Anti-Fluorochrome MicroBeads, respectively.

    A cocktail o antibodies can also be used to isolate or deplete a

    number o cell types concurrently.

    Since indirect labeling amplies the magnetic label, it may be

    the method o choice or magnetic separation according to

    dimly expressed antigens.

    MACS Magnetic Labeling

    Antibody MicroBead

    Streptavidin MicroBead

    Biotinylated antibody

    Fluorochrome-conjugated antibody

    Anti-Immunoglobulin MicroBead

    Streptavidin MicroBead

    Anti-Fluorochrome MicroBead

    Anti-Biotin MicroBead

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    Positive selection strategy Untouched isolation

    Positive selection means that the desired target cells are

    magnetically labeled and isolated as the magnetically retained

    cell raction.

    A positive selection strategy should be considered or:

    excellent purity, especially or rare cell enrichment,

    excellent recovery, and

    ast results.

    Positive selection is the most direct and specic way to isolate

    the target cells rom a heterogenous cell suspension. Binding

    o antibodies (which are part o the MicroBeads) to the cell

    surace does not aect viability or unction o the cells. Both

    ractions, labeled and unlabeled, can be recovered and used.

    Due to their composition o iron oxide and polysaccharide,

    MicroBeads are biodegradable and typically disappear ater a

    ew days in culture.

    MACS Separation Strategies

    Untouched isolation is perormed by depletion o undesired

    cells. Non-target cells are magnetically labeled and eliminated

    rom the cell mixture. The non-magnetic, untouched cell ractioncontains the target cells.

    Untouched isolation should be considered:

    or removal o unwanted cells,

    i no specic antibody is available or target cells,

    i binding o the antibody to the target cells is not desired, or

    or subsequent isolation o a cell subset by means o

    positive selection.

    For many dierent cell types Miltenyi Biotec oers optimized

    MACS Cell Isolation Kits containing pre-titrated cocktails o

    antibodies directed against non-target cells.

    Magnetic separationUndesired cells are retained in a

    MACS Column placed in a MACS


    The target cells pass through the

    column and are collected as the

    enriched, unlabeled cell raction,

    depleted o non-target cells.

    Magnetic labeling

    Non-target cells are magnetically

    labeled with a biotinylated antibody

    cocktail and Anti-Biotin MicroBeads.

    Magnetic separationCells are separated in a

    MACS Column placed in a

    MACS Separator.

    The low-through raction can

    be collected as negative raction

    depleted o the labeled cells.

    Magnetic labeling

    Cells o interest are magnetically

    labeled with MACS MicroBeads.

    Elution of the labeled cell fraction

    The column is removed rom the

    separator. The retained cells are

    eluted as the enriched, positively

    selected cell raction.

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    Sequential sorting (1):Depletion followed by positive selection

    Sequential sorting (2):MultiSort strategy

    Cell subsets can be isolated by rst depleting the non-target

    cells and then positively selecting the cell subsets o interest.

    This strategy is useul i undesired cells in the cell suspensionexpress the same antigen which is used or positive selection o

    the target cells. For isolation o extremely rare cells, it also can

    be useul rst to deplete non-target cells rom the suspension.

    Positive selection can then be carried out with the pre-enriched

    raction to obtain very pure cells.

    With MACS MultiSort Kits high numbers o cells, characterized

    by multiple cell surace markers, can be sorted easily. Even rare

    cells can be enriched very efciently.Multi-parameter sorting with MACS MultiSort Kits allows

    sequential positive selections o cells. The target cells are

    rst labeled with MACS MultiSort MicroBeads and positively

    selected or the rst parameter. Then the cells are incubated

    with the MultiSort Release Reagent which enzymatically

    removes the MicroBeads rom the antibodies. In the next

    step, the target cells are magnetically labeled with MACS

    MicroBeads directed against a subset marker to be positively

    selected a second time.

    MACS Separation Strategies

    1st magnetic labeling

    Non-target cells are

    magnetically labeled with a

    biotinylated antibody

    cocktail and Anti-Biotin


    1st magnetic separation

    Undesired cells are

    retained in a MACS

    Column placed in a

    MACS Separator while

    the unlabeled cells

    pass through.

    2nd magnetic labeling

    Target cells are magnetically

    labeled with MicroBeads

    according to a subset marker.

    2nd magnetic separation

    Target cells are

    retained in the column

    while unlabeled cellspass through.

    Ater the column is removed

    rom the separator, the

    target cells are eluted as the

    enriched, positively selected

    cell raction.

    1st magnetic labeling

    Cells o interest are

    magnetically labeled with

    MultiSort MicroBeads.

    1st magnetic separation

    Target cells are magnetically

    isolated by positive


    Release of magnetic


    MultiSort MicroBeads are

    enzymatically released.

    2nd magnetic labeling

    Cell subset o interest is

    labeled with MACS

    MicroBeads according to asecond marker.

    2nd magnetic separation

    Target cells are positively

    selected a second time.

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    Miltenyi Biotec provides products and services worldwide. Visit to nd your nearest Miltenyi Biotec contact.

    The CliniMACS System components: Instruments, Reagents, Tubing Sets, and PBS/EDTA Buer are manuactured and controlled under an ISO 13485 certied quality system. In Europe, the CliniMACS Systemcomponents areavailable as CE-marked medical devices. In the USA, the CliniMACS System components including t he CliniMACS Reagents are available or use only un der an approved Investigational New Drug(IND) application or Investigational Device Exemption (IDE). CliniMACS MicroBeads are or research use on ly and not or use in humans. autoMACS and CliniMACS are registered trademarks o Miltenyi Biotec

    GmbH. MiniMACS and QuadroMACS are trademarks o Miltenyi Biotec GmbH. Unless otherwise specically indicated, Miltenyi Biotec products and services are or research use only and not or therapeuticor diagnostic use. Copyright 2010 Miltenyi Biotec GmbH. All rights reserved.



    Miltenyi Biotec oers a wide variety o products or magnetic

    cell isolation in basic research as well as or clinical-grade cellseparationsproviding the perect tools or translational

    research. The continuously growing portolio also includes

    instrumentation and numerous antibodies or ow cytometry,

    as well as products or sample preparation, cell activation and

    expansion, and molecular analysis.

    To nd out more, visit our homepage at

    Germany/Austria/SwitzerlandMiltenyi Biotec GmbHFriedrich-Ebert-Strae6851429 Bergisch GladbachGermanyPhone +49 2204 8306-0

    Fax +49 2204 [email protected]

    USA/CanadaMiltenyi Biotec Inc.2303 Lindbergh StreetAuburn, CA 95602, USAPhone 800 FOR MACSPhone +1 530 888 8871Fax +1 530 888 [email protected]

    AustraliaMiltenyi BiotecAustralia Pty. Ltd.Unit 16A, 2 Eden Park DriveNorth Ryde, NSW 2113AustraliaPhone +61 2 8877 7400

    Fax +61 2 9889 [email protected]

    BeneluxMiltenyi Biotec B.V.Postbus 85183, 3508 AD UtrechtThe [email protected]

    Customer service NetherlandsPhone 0800 4020120Fax 0800 4020100Customer service BelgiumPhone 0800 94016Fax 0800 99626Customer service LuxembourgPhone 800 24971Fax 800 24984

    ChinaMiltenyi Biotec Trading(Shanghai) Co., Ltd.Shanghai OfceFareast International Plaza ARm. 2301, No. 319 Xianxia Rd.Shanghai 200051, P.R. China

    Phone +86 21 62351005Fax +86 21 [email protected]

    FranceMiltenyi Biotec SAS10 rue Mercoeur75011 Paris, FrancePhone +33 1 56 98 16 16Fax +33 1 56 98 16 [email protected]

    ItalyMiltenyi Biotec S.r.l.Via Persicetana, 2/D40012 Calderara di Reno (BO)ItalyPhone +39 051 6 460 411Fax +39 051 6 460 499

    [email protected]

    JapanMiltenyi Biotec K.K.Nittsu-Eitai Building 5F16-10 Fuyuki, Koto-ku, Tokyo135-0041, JapanPhone +81 3 5646 8910Fax +81 3 5646 8911

    [email protected]

    SingaporeMiltenyi BiotecAsia Pacic Pte Ltd.100 Beach Road#28-06 to 28-08 Shaw TowerSingapore 189702Phone +65 6238 8183Fax +65 6238 [email protected]

    SpainMiltenyi Biotec S.L.C/Luis Buuel 2Ciudad de la Imagen28223 Pozuelo de Alarcn(Madrid), SpainPhone +34 91 512 12 90

    Fax +34 91 512 12 [email protected]

    United KingdomMiltenyi Biotec Ltd.Almac House, Church LaneBisleySurrey GU24 9DR, UKPhone +44 1483 799 800Fax +44 1483 799 [email protected]