Breaking new grounds with MACS® Technology

Unmatched sensitivity for RNA research

Choose from manual or automated 96-well protocols

Save time by sensitive one-step mRNA isolation and cDNA synthesis

Unique kit for single-cell gene expression profiling

MACS® Technology by Miltenyi BiotecComprehensive solutions from cell to molecular analysis

2

Sample preparation

• Fastandgentlesamplepreparationfromanytissue

• Getviablesinglecellsforcellseparation,analysis,orcultivation

• Getcellhomogenatesformolecularanalysis

Cell separation

• MACS®Technology,thegoldstandardincellseparation

• Manualorautomaticseparationofvirtuallyanycelltype

• Standardizationwithautomatedcellseparation

Cell culture

• Serum-freemediumandsupplementforlong-termviability

• Premium,GMP,andresearchgradecytokines

Imaging

• Contrastagentsoptimizedforsmallanimalimaging

• MRI,opticalimaging,CTandultrasound

Cell analysis

• Titratedhigh-qualityantibodiesandbrilliantfluorochromes

• Easy-to-usebestinclassflowcytometers

Molecular analysis

• Fastisolationoffunctionalmitochondria

• Sensitiveproteinisolationevenfromsmallsamples

• EfficientmRNAisolationandamplificationfromsmallsamples

• GenomicServicesforgeneandmicroRNAexpressionprofilingandarrayCGH

Sinceitsintroductionin1989,MACS®Technologyhasbecomethegoldstandardforcellseparation.Nowadays,MiltenyiBiotecstandsformorethancellseparation,offeringover1000innovativeresearchproductsforbiomedicalresearchandlifesciences.TheMACSProductportfolioincludesinstrumentsand

reagentsforsamplepreparation,cellseparation,cellanalysis,cellculture,andmolecularbiology.Overthelast20years,researchershavepublishedmorethan14,500paperswithourproducts.MiltenyiBiotechasastrongcommitmenttoconstantlydevelopnewproductsforcurrentandfutureresearch.

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MACSmolecular products MACS® Technology for molecular applications

Products for highly sensitive RNA analysisInmanyresearchfields,geneexpressionanalysisisastandardapproachtogainabetterunderstandingofparticularbiologicalprocesses.ResearcherseitherfocusonsinglegenesviaPCRanalysis,ortheymonitorwholegenomesusingmicroarrayanalysis.

AccurategeneexpressionanalysesdependonmRNAisolationmethodsthatcircumventcommonpitfalls,suchasDNAcontaminationsordegradationofRNAduringisolation.SignificantamountsofmRNAareoftenlostduringprecipitationandwashingsteps.Inparticular,whensamplematerialislimitedtoafewcellsareliabletechnologyforefficientmRNAisolationandcDNAsynthesisisrequired.

MACS®Technologytakescareofthisnecessity:OutstandingRNArecoveryduringisolationisachievedbyutilizingthesuperparamagneticMicroBeads.These50-nmparticlesinstantlybindtotheirtargetpoly-AtailandallowmRNAisolationevenfromasinglecell.Theinnovativein-columncDNAsynthesisreduceslossofmaterialandfurtherincreasesthesensitivityandreliabilityofyourRNAanalysis.

ThenewµMACS™SuperAmp™Kitallowswholegenomeanalysisfromjustonecellupto10,000cells.Thus,MiltenyiBiotecproductsforRNAresearchareideallysuitedforassayingsmallsamples.

Contents

4 How MACS® Technology works Forone-stepmRNAisolation andcDNAsynthesis

6 mRNA isolation and cDNA synthesis ManualsamplepreparationforPCRanalysis

8 96-well mRNA isolation and cDNA synthesis Automatedsamplepreparation forPCRanalysis

10 Single-cell gene expression profiling SuperAmp™Technologyforunmatched sensitivityinmicroarrayanalysis

12 Microarray Services for RNA research Expertconsultationrightfromthestart

13 More products for RNA research Samplepreparationandstabilization, referenceRNA,andin situhybridization

14 Product overview Placeyourorderbyfax,phone,oronline

15 References Selectedfrommorethan14,500papersusing productsfromMiltenyiBiotec

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Fig. 1: Principle of MACS Technology for mRNA isolation and cDNA synthesis

CellsortissuearelysedusingLysis/BindingBuffer.

ClearingoflysateusingLysateClearColumn.

AdditionofµMACS™Oligo(dT)MicroBeadstothelysate.

The operating principle SuperparamagneticµMACS™Oligo(dT)MicroBeadsareappliedtothecelllysateandinstantlybindtotheirtargetmRNA.Subsequently,themagneticallylabeledmRNAisisolatedandpurifiedinaMACSColumnplacedinthemagneticfieldofaMACSSeparator.Reversetranscriptiontakesplaceinthesamecolumn.Afterthoroughwashing,purecDNAiseluted(fordetails,pleaserefertofigure1).

How you benefit from MACS® Technology BenefitfromMACSTechnologyforone-stepmRNAisolationandcDNAsynthesis:

Outstanding sensitivityAfewcellsareenough.

High purityNocontaminationwithgenomicDNA.

High recoveryPreservesamplematerialduetoomissionofcentrifugationstepsorbufferremoval.

Reproducibility and reliabilityOne-stepcDNAsynthesisprocedurecanbeeasilyautomated.

How MACS® Technology worksFor one-step mRNA isolation and cDNA synthesis

5 min

3 min

cDNAsynthesismixisappliedontothecolumnandcDNAisgenerated.

LysateisappliedtoaµColumninthermoMACS™Separator.WashofmRNA.

0 min

6 min

ElutionofcDNA/mRNAhybrid.

PurificationandreleaseofcDNA/mRNAhybrid.

1 h

1 min

cDNA in 1 h 30 min

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The components

1. µMACS™ Oligo(dT) MicroBeads • Superparamagnetic—onlymagnetizedina magneticfield

• Smallinsize—only50nmindiameter

• Non-sedimentingwithextremelyhighreaction kinetics—instantlybindtotargetmRNA

Benefit:Outstandingsensitivity—mRNAcanbeisolatedfromasfewasasinglecell.

2. MACS® Column• Packedwithsteelspheresthatenhancethemagnetic field—essentialtoretainnanometer-sizedµMACS™ MicroBeadsboundtotargetmRNA

• Buffersrunbygravityflow,thus,noneedfor centrifugationorbufferremovalsteps.Lossoftarget isprevented.

• Thoroughrinsingprocedure

Benefit:Highrecoveryandpurity.

3. MACS SeparatorsMACSSeparatorsarepermanentmagnets.TheheatablethermoMACS™Separatorwasdevelopedespeciallyforin-columnenzymaticreactionssuchascDNAsynthesisofisolatedmRNA.Theone-stepapproachreduceslossofmaterialbyavoidingtube-to-tubetransfer.

Benefit:Highrecovery

Automated 96-well processingTheprocedurecaneasilybescaled-uptoa96-wellformatutilizingtheMultiMACS™M96thermoSeparator.Afullyautomatedapproachisachievedbyintegratingthisbenchtopinstrumentintoaroboticpipettingsystem.

Benefit:Highreproducibilityandreliability.

Fig. 5: thermoMACS SeparatorFig. 4: MultiMACS M96thermo Separator

Fig. 3: MACS Column

Fig. 2: µMACS Oligo(dT) MicroBeads

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mRNA isolation and cDNA synthesisManual sample preparation for PCR analysis

Fig. 1: µMACS mRNA Isolation Kit

Fig. 3: thermoMACS™ Separator

M 1 2 3kb

9.5—7.5—

4.4—

2.4—1.4—

0.2—

Fig. 2: mRNA was isolated from total RNA of a hybridoma cell line Agarosegellane1showstotalRNA(Trizol™,Invitrogen),lane2containsflow-throughwashbuffer,andlane3reflectsmRNAisolatedbyMACSTechnology;M,marker.

Outstanding sensitivity — a few cells are enoughTheµMACS™ mRNA Isolation Kitsyieldfull-length,intactmRNAfromupto107cells(fig.2).Evenwhenstartingwithsmallcellnumbers,thismagneticbead–basedtechnologyensuresreliableresults.

Duetothefastreactionkineticsoftheextremelysmall(50nm)MicroBeads,mRNAmoleculesareinstantlyboundandhigh-puritymRNAcanbesuccessfullyisolatedfromverysmallsamples.

High purityMACS®Technologyenablesathoroughin-columnwashingprocedure.PremiummRNAcanbeisolatedwithoutanygenomicDNAcontamination(fig.5B).

Direct isolation of mRNA from various samplesHigh-qualitymRNAcanbeisolateddirectlyfromvariousbiologicalmaterials.Itworkslikeacharmforallcelllines,forprimarycellsandalsofortissue.

mRNA in 15 minutesmRNAisolationdirectlyfromcell,tissue,orbloodsamplestakesonly15minutes.Withinafurther75minutesthecDNAcanbeeasilysynthesizedandpurifiedinthesamecolumnthatwasusedformRNAisolation.

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500—400—300—200—100—

Unique one-step mRNA isolation and cDNA synthesis InsteadofelutingthepurifiedmRNAfromthecolumn(refertopage4),afirst-strandcDNAsynthesiscanbeperformeddirectlyinthecolumnemployingtheµMACS™One-stepcDNAKitandthethermoMACS™Separator.Thelatterisapermanentmagnetthatcanbeheatedto37°Cor42°C.

Five cells are enough for PCR analysis Therevolutionaryin-columncDNAsynthesis(describedonpage4)significantlyreduceslossofmRNAandcDNA.MagneticallyboundmRNAisdirectlyreversetranscribedinthecolumnusingahighlyefficientreversetranscriptase.Afterfirst-strandsynthesis,unwantedcomponentsarewashedawaywhilethecDNAisretainedinthecolumn.

Thisuniquetechnologypreservessamplematerialateachstep,asmRNAisolation,cDNAsynthesisandpurificationareallperformedwithinthesamecolumn.Evenwhenstartingwithsmallsamples,suchasfivecells,reliablecDNAsynthesiscanbeachieved(fig.5).

“It is highly reliable … and yields a large amount of pure product.”

“In contrast to the other techniques, genomic DNA amplification was detected in none of the samples that had been processed by magnetic bead isolation.”

Macket al.(2007)CytometryA.71:404–409.

Fluo

rescen

ce(F1)

Cyclenumber

ab

c

d

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0.0

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M1234

A)mRNAwasisolatedandreversetranscribedintocDNAbyμMACSOne-stepcDNAKit(a:500cells;b:5cells)orbyconventionaltubemethods(c:500cells;d:5cells).e:non-templatecontrol.10%ofeachcDNAwasanalyzedbyquantitativePCRusingintron-spanningprimers.

B)μMACSOne-stepcDNAsynthesis(lanes1and2:500Jurkatcells;lanes3and4:5Jurkatcells,M:100bpladder).50%ofeachcDNAwasusedinastandardPCRreaction.A372bpfragmentofGAPDHwasamplifiedwithintron-spanningGAPDHprimers(genomicDNAwouldgivea476bpfragment)andanalyzedbyagarosegelelectrophoresis.

Fig. 5: Sensitive generation of PCR template using µMACS TechnologyPCRanalysisofGAPDHfragmentusingmRNAisolatedfrom500or5JurkatcellsbyquantitativePCR(A)andagarosegelelectrophoresis(B).

Fig. 4: The thermoMACS™ Separator can be heated to 37 °C or 42 °C

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96-well mRNA isolation and cDNA synthesisAutomated sample preparation for PCR analysis

Automated 96-well mRNA isolation Formulti-specimenPCRscreeningtheprovenMACS®TechnologyformRNAisolation(refertopage6)canbescaleduptoa96-wellformat.

TheMultiMACS™ mRNA Isolation KitsareusedincombinationwiththeMultiMACSM96Separator.Thisbenchtopinstrumentcontainsa96-wellmagnetandallowsthesimultaneousmRNApurificationofmultiplesamplesinlessthan45minutesdirectlyfromcellsortissues.

TheMultiMACS M96 Separatorcanbeoperatedmanuallyatthebenchorautomaticallybyintegratingitwitharoboticpipettingsystem(figs.4and5).

High reliability and reproducibilityUsingtheMultiMACSmRNAIsolationKitsandtheMultiMACScDNASynthesisKits(refertonextpage),thevariationinyieldandqualityofmRNAandcDNAaswellasreal-timePCRthresholdsareverylow(figs.2and3).

“We usually process several dozen samples. Now, with the MultiMACS M96thermo Separator we can do parallel cDNA synthesis from up to 96 samples and save a lot of time and costs.”

Prof.C.Wolfrum,ETHZurich,Switzerland

1.60

1.45

1.30

1.15

1.00

0.85

0.70

0.55

0.40

0.25

0.10

-0.051 2 3 4 5 6 7 8 9 10 11 12

1.45–1.60

1.30–1.45

1.15–1.30

1.00–1.15

0.85–1.00

Fig. 2: Quantification of 96 mRNA samples UsingtheMultiMACSM96SeparatorcombinedwiththeMultiMACSmRNAIsolationKit,100μgofmouselivertotalRNAwaspurifiedandquantified.Averageyield:1.3μgmRNA;coefficientofvariationof96samples:5.2%.

Fig. 3: Quantitative RT-PCR of 96 samples using the MultiMACS System 100μgtotalRNAwaspurifiedfrommouseliverandsubsequentlyreversetranscribedbyusingtheMultiMACScDNASynthesisKitandtheMultiMACSSeparator.Afterreversetranscription,quantitativeamplificationofthehousekeepinggeneGAPDHwasmeasuredbyreal-timePCR.Meancyclethreshold:12.0;coefficientofvariation:2.0%.

Fig. 4: Manual sample processing Fig. 5: Automatic sample handling

Fig. 1: MultiMACS M96thermo Separator for 96-well mRNA isolation and cDNA synthesis

1 10 20 30 5040

Cycle number

5 15 25 35 45

10

11

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1–2

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ta R

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Automated 96-well cDNA synthesis TheMultiMACS™M96SeparatorisalsoavailablewithaheatablemagnetandisthentermedtheMultiMACS M96thermo Separator(fig.1).Thus,theone-stepmRNAisolationandcDNAsynthesisprocedurecanbescaled-uptofully-automated,parallelprocessingof96samples.

UsetheMultiMACS cDNA Synthesis KitsincombinationwiththeMultiMACSM96thermoSeparatortoautomateyourmRNAisolationandcDNAsynthesisprotocolandbenefitfromtheadvantagesofMACS®Technology:

• Onestep—mRNAisolation,cDNAsynthesisand purificationareperformedinonecolumn

• Extremelysensitive—fivecellsareenoughforPCRanalysis

• Highpurity—nocontaminationofgenomicDNA

• Highreliabilityandreproducibility

• Nocross-contamination

No cross-contamination due to contact-free pipettingAhallmarkofMACSTechnologyisthegravity-drivencolumnflow.Thisnotonlyavoidscentrifugationstepsbutalsoenablescontact-freepipetting(fig.6)asthereisnoneedtoremovebuffersafterwashingsteps.Thus,theriskofcross-contaminationisprevented(fig.7).

“The sensitivity of this in-column cDNA technology is truly benchmark, and I don’t know any other technology that allows processing of up to 96 samples resulting in such high-quality cDNA — even without any genomic DNA contamination!”

Prof.C.Wolfrum,ETHZurich,Switzerland

Fig. 7: Analysis of cDNA synthesized with the MultiMACS System mRNAextractedfrom15mgmouseliverwaspurifiedusingtheMultiMACSKitandSeparator.Liversamplesandcontrols(lysis/bindingbuffer)wereloadedintoMultiMACSSamplePlatewellsinanalternatefashion,i.e.,sample–control–sample–control,etc.RT-PCRwassubsequentlyperformedonthecontentsofeachplatewellwithGAPDHprimers(35cycles).1µLofeachcDNAproduct/controlwasresolvedbyagarosegelelectrophoresis(2%).Itisevidentthatnocross-contaminationoccurredbetweencontrolandliversamplesusingtheMultiMACSSystem.

Fig. 6: Contact-free pipetting for reduced risk of cross-contamination

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The optimal solution for all sample sizes — even for single cells MiltenyiBiotec’sMicroBead-basedisolationprocedureofhigh-puritymRNAisideallysuitedformicroarrayanalysis.Kitsareavailableforallsamplessizes(table1)from107cellsorequivalentamountsoftissueortotalRNAtoonlyasinglecellwhenusingtheµMACS™SuperAmp™Technology.

µMACS™ SuperAmp™ Kit — a single cell is enough !Basedonthewell-establishedMACS®TechnologytheµMACSSuperAmpSystemallowshighlysensitivemRNAisolation,cDNAsynthesis,andamillionfoldamplificationofthemRNA-derivedcDNAbyglobalPCR.TheprincipleoftheµMACSSuperAmpProcessisillustratedinfigure2.

The unique µMACS SuperAmp Protocol combines several features• Unmatchedsensitivity

• Highreproducibility

PCRbiasisavoidedbytheuseofuniformprimersand uniformannealingconditionsforalltranscripts.In addition,theconsistentlengthofgeneratedcDNA fragmentsavoidsPCRbias.Thisprocesspreservesthe relativetranscriptabundanceinthesamplewith virtuallynonon-specificamplificationproduct.

• Muchfasterandmorereliablethantworoundsof T7amplification

• Easytouse

• Robustandreliablesystem

Fig. 1: Linearity analysis of the SuperAmp Protocol cDNAswereamplifiedfrom1,10,100,1,000and10,000Jurkatcells,andfrom1000RajicellsusingtheSuperAmpSystem.ToevaluatelinearityeachamplifiedJurkatcDNAproductwaslabeledandcomparedtothetargetRajicDNAproductintwo-colorPIQOR™ImmunologyMicroarrayexperiments.(A)showsthecalculatedpearsoncorrelationcoefficient(r)ofindependentrepeatsforeachJurkatcellbatch.(B)showshierarchicalclusteringanalysis:noobviousgroupingofrepeatsorcellnumbersisevident.

10.90.80.70.60.50.40.30.20.10

Numberofcells

0.98 0.96 0.96 0.94 0.7

Correlation

coefficien

t(r)

10,000 1,000 100 10 1

A)

1 ce

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)

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1)

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cel

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(3)

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CD19

CD20

CD3D

CD4

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)

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cel

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1 ce

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7.5 0.0 7.5

B)

Single-cell gene expression profiling

SuperAmp™ Technology for unmatched sensitivity in microarray analysis

µMACS One-step cDNA Labeling Kit

µMACS One-step T7 Template Kit

µMACS SuperAmp Kit

Upto107cells 1×106–5×104cells 104–asinglecell

Upto30mghumanandanimaltissue(100mgplanttissue)

6mg–500µgtissue

200µg–5µgtotalRNA

10µg–250ngtotalRNA

100ng–10pgtotalRNA

Noamplification

1000-foldamplification

Millionfoldamplification

Table 1: Amplification efficiencies of μMACS Products

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Fig. 2: Principle of the µMACS SuperAmp Technology µMACSSuperAmpTechnologyenablesgeneexpressionprofilingfromeventhetiniestamountsofsamplematerial.

KlenowlabelingwithrandomprimerandpurificationoflabeledamplifiedcDNA

3 h

0 min

AdditionofµMACS™SuperAmp™MicroBeadsandmRNAbindingcTAG

cTAG

ApplicationoflysatetoaµColumnplacedinathermoMACS™SeparatorandwashingofmRNA

5 min

1 h 10 min

In-columncDNAsynthesisofsmalluniform-sizedcDNAfragmentscTAG cTAG

TailingofcDNA1 h 30 minTAGTAG cTAG cTAG

4 h

Single-primerPCRamplificationandpurificationofcDNA

TAGTAG

Primer TAG Primer TAG

TAGTAG

Primer

cTAG

Primer

Primer

Primer

cTAG cTAG

PrimerPrimer

TAGTAG

Primer TAG TAGPrimer

cTAG cTAG

cTAG

LysisofcellsortissueusingLysis/BindingBuffer

25 min

NucleicacidtagTAG cTAG Complementarytag

10 h 10 min total

Fluorescentorbiotinlabel

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Ten years of microarray experienceMiltenyiBiotechastenyearsofmicroarrayexperienceandoffersahugevarietyofgenomicsservices.Since2003,wearealsoanofficiallycertifiedAgilentServiceprovider.

Thereisnoneedtoestablishmicroarraytechnologyinyourownlaboratory—simplysendcells,tissue,orbloodsamplestoourMicroarrayServicesDepartmentandreceivereliableresultsanddetaileddocumentationinreturn.

Flexible expression profiling servicesMiltenyiBiotecprovidesawiderangeofcost-effectivemicroarrayservices:

• microRNAexpressionanalysisbasedon miRXplore™Microarrays

• Agilentwholegenomeexpressionanalysis

• Agilentarray-CGH

SuperAmp™ Service for one-cell microarray experimentsTheSuperAmpServiceisanextensionoftheMicroarrayServicesandallowssuccessfulgeneexpressionprofilingfrom1–10,000cells.

FinddetailedinformationonourextensiveGenomicServicesatwww.miltenyibiotec.com/genomic-services

Microarray Services for RNA research Expert consultation right from the start

Send sample — receive results

Technical support for experimentaldesign and microarray selection

• microRNAmiRXplore™Microarrays

• AgilentWholeGenomeMicroarrays

RNA extraction and quality control

Optional:

• Amplificationandqualitycontrol

• SuperAmpService*:1–10,000cells

Synthesis and purification of fluorescently labeled probes

Microarray hybridization

Image capture and analysis of primary data

Optional: Bioinformatics Services**

• Pre-processing

• RatioBuilding

• Cluster

• DiscriminatoryGenes

• FunctionalGrouping

• PathwayAnalysis

Results and report

• DataonCD-ROM

*microRNAscannotbeamplifiedwiththeSuperAmpService.**PleaseinquireformicroRNABioinformaticsServices.

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gentleMACS™ DissociatorsTheisolationofsubcellularmaterialsuchastotalRNAormRNAfromtissuesorcellsrequiresfastandthoroughhomogenizationofthestartingmaterial.ThegentleMACS™OctoDissociatorandgentleMACS™Dissociator(fig.1)provideoptimizedhomogenizationprogramsthatmeettheserequirements.Forfurtherinformation,refertowww.gentlemacs.com

PrepProtect™ Stabilization BufferSimplybyaddingthenon-toxicPrepProtect™Solution,RNA,DNA,orproteinisstabilizedincellortissuesamplesandisavailableforreliabledownstreamapplications.

PrepProtectStabilizationBufferpreventsRNAdegradationwheneverquick-freezingordirectanalysisaftersamplecollectionisimpossible,forexample,whensamplesareshippedwithoutdryiceorfrozentissueisweighedanddissected.

Fig. 1: gentleMACS™ Octo Dissociator and gentleMACS Dissociator

More products for RNA researchSample preparation and stabilization, reference RNA, and in situ hybridization

Cell type–specific total RNAHighlypuretotalRNAfromdistincthematopoieticcellpopulationssuchashematopoieticprogenitorcells(CD34+)orregulatoryTcells(CD4+CD25+)isreadytouseingeneexpressionprofilingexperiments,genecloning,orasareferenceforfurtheranalyses.

EasyProbe Transcription Templates for in situ hybridization or Northern blotsSequence-verifiedEasyProbeTranscriptionTemplatesarelinear,double-strandedDNAtemplatesforin vitrotran-scriptiontogenerategene-specificRNAprobesforin situhybridizationorNorthernblotanalysis.

Thetrancriptiontemplatesarespecificforhumanskin-relatedandmouseneuralmRNAs.Solubilizedafterdelivery,thetemplatecanbelabeledwitheitherfluorophores,digoxygenine,biotin,orradioactiveisotopes.

Fordetailedinformationoncelltype–specifictotalRNAsandEasyProbeTranscriptionTemplates,refertowww.miltenyibiotec.com

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Product overview Place your order by fax, phone, or online !

Products Capacity Order no.

mRNA isolation and cDNA synthesis

µMACSmRNAStartingKit 20reactions 130-075-202

µMACSmRNAIsolationKit,SmallScale 10reactions 130-090-276

µMACSmRNAIsolationKit,SmallScale 20reactions 130-075-201

µMACSmRNAIsolationKit,LargeScale 4reactions 130-090-277

µMACSmRNAIsolationKit,LargeScale 8reactions 130-075-101

µMACSmRNAIsolationKit,ForTotalRNA 8reactions 130-075-102

µMACSOne-stepcDNAStartingKit 20reactions 130-091-989

µMACSOne-stepcDNAKit 20reactions 130-091-902

96-well mRNA isolation and cDNA synthesis

MultiMACSmRNAIsolationKit(12×8) 96reactions 130-092-520

MultiMACSmRNAIsolationKit(4×96) 384reactions 130-092-519

MultiMACScDNASynthesisKit(12×8) 96reactions 130-094-410

MultiMACScDNASynthesisKit(4×96) 384reactions 130-094-408

cDNA labeling and amplification

µMACSOne-stepcDNALabelingStartingKit 20reactions 130-092-521

µMACSOne-stepcDNALabelingKit 20reactions 130-092-443

µMACSOne-stepT7TemplateStartingKit 20reactions 130-092-943

µMACSOne-stepT7TemplateKit 20reactions 130-092-866

µMACSSuperAmpStartingKit 10reactions 130-093-251

µMACSSuperAmpKit 10reactions 130-093-242

RNA stabilization buffer

PrepProtectStabilizationBuffer 10mL 130-092-643

PrepProtectStabilizationBuffer 100mL 130-092-642

Products Order no.

Equipment

µMACSSeparationUnit 130-042-602

thermoMACSSeparationUnit 130-091-136

MiniMACSSeparationUnit 130-042-102

OctoMACSSeparationUnit 130-042-109

MACSMultiStand 130-042-303

µColumns(20reactions) 130-042-701

MColumns(10reactions) 130-042-801

Products Order no.

Equipment for 96-well approaches

MultiMACS96Separator 130-091-937

MultiMACSM96thermoSeparator 130-094-534

Multi-8Columns,molecular(12×8) 130-092-444

Multi-96Columns,molecular(4×96) 130-092-445

Multi-8Filters 130-092-546

Multi-8FiltersandFrames 130-092-548

Multi-96Filters 130-092-547

DeepWellBlock,2.5mL 130-092-549

ReferencesSelected from more than 14,500 papers using products from Miltenyi Biotec

15

Read for yourself how MACS® Technology performed in a direct comparison of methods for PCR template generation:

Macketal.(2007)CytometryA.71:404–409.ComparisonofRNAyieldfromsmallcellpopulationssortedbyflowcytometryapplyingdifferentisolationprocedure.

µMACS™ One-step cDNA TechnologyMhyreet al. (2009)Blood13:649–58.

Shakibet al. (2009)J.Immunol.182:130–137.

Marcondeset al.(2008)Proc.Natl.Acad.Sci.USA105:2865–2870.

Sag et al. (2008)J.Immunol.181:8633–8641.

Sachdevaet al. (2008)J.Immunol.181:2887–2897.

Mack et al. (2007) Cytometry A. 71: 404–409.

Molleyet al. (2007)BreastCancerRes.Treat.112:297–307.

Watkinset al. (2007)J.Immunol.178:1357–1362.

Chewninget al. (2007)J.Immunol.179:854–868.

Jenkinsonet al. (2007)Blood109:954–60.

Rossiet al. (2007)J.Exp.Med.204:1267–1272.

Nardmannet al. (2007)Mol.Biol.Evol.24:2474–2484.

Learnet al. (2006)Clin.CancerRes.12:7306–7315.

Fecciet al. (2006)CancerRes.66:3294–3302.

O’Neillet al. (2006)Nat.Genet.38:835–841.

µMACS™ SuperAmp™ TechnologyBisselset al. (2011)StemCells29(5):847-857

Gueroutet al. (2010)Glia(58):1570-1580

Biagioliet al. (2009)PNAS106(36):15454-15459

Stehling-Sunet al.(2009)NatureImmunol.10(3):289-296.

Paulsenet al. (2009)J.Neurosci.Methods177(1):87-93.

Liet al. (2009)Differentiation77(1):95-102

Hardtet al. (2008)Mol.Cell.Neurosc.39:418-428.

Auffrayet al. (2007)Science317:666-670.

Appayet al. (2007)JournalofImmunology179:7406-7414.

Zeytunet al. (2007)Adv.Exp.Med.Biol.598:342-357.

miRXplore™ Microarray TechnologyBisselset al. (2011)StemCells29(5):847-857

Stittrichet al. (2010),Nat.Immunol.11(11):1057-1062

Bissels,Tuschlet al. (2009),RNA15(12):1-10

Landgraf,Tuschlet al. (2007)Cell129:1401-1414[10484]

Landgraf,Tuschlet al. (2007)Cell129,SupplementalData

EasyProbe Transcription TemplatesWenzelet al. (2008)J.Invest.Dermatol.128:67–78.

Hardtet al. (2008)Mol.Cell.Neurosci.39:418–428.

PrepProtect™ Stabilization BufferMacket al. (2007)CytometryA.71:404–409.

130-09

0-219.11

MiltenyiBiotecprovidesproductsandservicesworldwide.Visitwww.miltenyibiotec.com/localtofindyournearestMiltenyiBioteccontact.

Unlessotherwisespecificallyindicated,MiltenyiBiotecproductsandservicesareforresearchuseonlyandnotfortherapeuticordiagnosticuse.MACS,theMACSLogo,μMACS,thermoMACS,MiniMACS,OctoMACS,gentleMACS,andMultiMACSareregisteredtrademarksortrademarksofMiltenyiBiotec.TrizolisatrademarkofInvitrogenCorp.Copyright©2011MiltenyiBiotecGmbH.Allrightsreserved.

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Australia MiltenyiBiotecAustraliaPty.Ltd.Unit16A,2EdenParkDriveNorthRyde,NSW2113,AustraliaPhone+61288777400Fax+61298895044macs@miltenyibiotec.com.au

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Customer service Netherlands Phone08004020120Fax08004020100Customer service BelgiumPhone080094016Fax080099626Customer service LuxembourgPhone80024971Fax80024984

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FranceMiltenyiBiotecSAS10rueMercoeur75011Paris,FrancePhone+33156981616Fax+33156981617macs@miltenyibiotec.fr

ItalyMiltenyiBiotecS.r.l.ViaPersicetana,2/D40012CalderaradiReno(BO)ItalyPhone+390516460411Fax+390516460499macs@miltenyibiotec.it

JapanMiltenyiBiotecK.K.Nittsu-EitaiBuilding5F16-10Fuyuki,Koto-ku,Tokyo135-0041,JapanPhone+81356468910Fax+81356468911macs@miltenyibiotec.jp

SingaporeMiltenyiBiotecAsiaPacificPteLtd.100BeachRoad#28-06to28-08ShawTower

Singapore189702Phone+6562388183Fax+6562380302macs@miltenyibiotec.com.sg

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United Kingdom MiltenyiBiotecLtd.AlmacHouse,ChurchLaneBisley,SurreyGU249DR,UKPhone+441483799800Fax+441483799811macs@miltenyibiotec.co.uk