M. Tech Biotechnology Syllabus w. e. f.

M. Tech

Biotechnology

Syllabus

w. e. f.

2019-2020

M.Tech. (BIOTECHNOLOGY)

Program Outcomes:

PO1. Engineering knowledge: Apply the advanced knowledge of mathematics,

science, engineering fundamentals, and an engineering specialization to the

solution of complex engineering problems.

PO2. Problem analysis: Identify, formulate, research literature, and analyse complex

biological problems reaching substantiated conclusions using basic principles

of biotechnology.

PO3. Design/development of solutions: Design solutions for complex engineering

and biotechnological problems and design relevant system components or

processes that meet up to the specific needs with appropriate consideration for

the public health and safety.

PO4. Conduct investigations of complex problems: Use research-based knowledge

and research methods including design of experiments, analysis and

interpretation of data, and synthesis of the information to provide valid

conclusions.

PO5. Modern tool usage: Create, select, and apply appropriate techniques,

resources, and modern engineering and IT tools including prediction and

modelling to complex engineering activities with an understanding of the

limitations.

PO6. The engineer and society: Apply reasoning informed by the contextual

knowledge to assess societal, health, safety, legal and cultural issues and the

consequent responsibilities relevant to the professional engineering practice.

PO7. Environment and sustainability: Understand the impact of the professional

engineering solutions in societal and environmental contexts, and demonstrate

the knowledge of, and need for sustainable development.

PO8. Ethics: Apply ethical principles and commit to professional ethics and

responsibilities and norms of the engineering practice.

PO9. Individual and team work: Function effectively as an individual, and as a

member or leader in diverse teams, and in multidisciplinary settings.

PO10. Communication: Communicate effectively on complex engineering activities

with the engineering community and with society at large, such as, being able

to comprehend and write effective reports and design documentation, make

effective presentations, and give and receive clear instructions.

PO11. Project management and finance: Demonstrate knowledge and

understanding of the engineering and management principles and apply these

to one’s own work, as a member and leader in a team, to manage projects and

in multidisciplinary environments.

PO12. Life-long learning: Recognize the need for, and have the preparation and

ability to engage in independent and life-long learning in the broadest context

of technological change.

Program Learning Outcomes

1. This program will aid the students to think originally, intellectualize and

solve various problems associated to the field of biotechnology.

2. At the end of this program students should be able to extract knowledge

through proper literature survey and experiments, research methodologies

so that they can help in the development of scientific community or

technology.

3. Program will aid the students to communicate effectively with the biotech

community with confidence. They should assuredly comprehend and create

effective research reports and documentation designing with keeping

appropriate standards intact.

4. This program will facilitate the students to acquire in-depth knowledge of

biotechnology with the ability to discriminate, evaluate, analyse and

synthesize the existing and new knowledge and to integrate the same in

doing new start-ups for participation in active discussions among the

scientific community.

S.

No. Subject

Code Subject L T P CIE ESE Total C

THEORY

1. MHU1002 Technical Communication 2 0 - 40 60 100 2

2. MBT1011 Bioprocess Engineering and

Technology 4 0 - 40 60 100 4

3. MBT1012 Biochemistry and Metabolic

Regulation 3 0 - 40 60 100 3

4. MBT1013 Computational and Structural

Biology 4 0 - 40 60 100 4

5. MBT1014 Advanced Immunology and

Immunological Techniques 4 0 - 40 60 100 4

PRACTICAL/TRAINING/PROJECT

6. MBT1508 Bioprocess Engineering Lab - - 2 80 20 100 1

7. MBT1509 Biochemistry and

Computational Biology Lab - - 2 80 20 100 1

8. MBT1504 Seminar-I - - 1 100 0 100 1

Total 17 0 5 460 340 800 20

M. Tech. Biotechnology I Year: I Semester

S.

No. Subject


THEORY

1. MBT2009 Recombinant DNA Technology 4 0 - 40 60 100 4

2. MBT2010 Advance Down Stream

Processing 3 0 - 40 60 100 3

3. MBT2011 Molecular Biology and Virology 4 0 - 40 60 100 4

4. --- Department Elective – I 3 0 - 40 60 100 3

5. --- Department Elective – II 3 0 - 40 60 100 3


6. MBT2510 Recombinant DNA Technology

Lab 0 0 2 80 20 100 1

7. MBT2511 Molecular Biology and Virology

Lab 0 0 2 80 20 100 1

8. MBT2512 Comprehensive Viva Voce 0 0 0 80 20 100 1

9. MBT2504 Seminar-II 0 0 1 100 - 100 1

Total 17 0 5 540 360 900 21

M. Tech. Biotechnology I Year: II Semester

S.

No.

Subject


THEORY

1. MBT3005 Industrial and Pharmaceutical

Biotechnology 4 0 - 40 60 100 4

2. MBT3006 Enzyme Engineering and

Technology 4 0 - 40 60 100 4

3. MBT3007

Ethical Issues in

Biotechnology and

Engineering

3 0 - 40 60 100 3

4. MBT3008 Technical Writing and

Literature Survey 2 0 - 20 30 100 2

5. --- Department Elective – III 3 0 - 40 60 100 3


6. MBT3507 Biochemical Preparations Lab 0 0 4 80 20 100 2

7. MBT3508 Project Work Phase-I 0 0 4 80 20 100 2

8. MBT3503 Seminar-III 0 0 1 100 0 100 1

Total 16 0 9 440 310 750 21

Every student or a group of 3-4 students will be given a mentor, as decided by a Committee constituted by the Director, IBST, based

on the inputs provided by the students, at the end of the II or beginning of III semester. Students will be asked to give their choice if

they are interested in doing the project at the university or outside the university at a recognized research institution. The university

will place the students who choose to do their projects outside the university at research institutes or universities of repute where high-

impact research is conducted. The students who choose to do their projects at the university will be asked to decide a supervisor from

the department. The supervisor will identify a suitable research topic and assign the same to the student. The supervisor will help the

student in literature survey, in making a research plan, and doing preliminary or validation experiments, etc as per the requirements of

the project.

M. Tech. Biotechnology II Year: III Semester

University Mandatory Non-Credit Course

1. XHUX601 Human Values and Ethics 2 - - 100 - 100 0

S.

No.

Subject



1. MBT4507 Project work Phase-II - - 32 50 50 100 16

Total 0 0 32 50 50 100 16

During the IV semester students will do their project work. The internal evaluation of students doing their projects

at the university will be conducted by attendance monitoring and research progress. The internal evaluation of

students doing their projects outside the university will be based upon the fortnightly/monthly reports sent via email

by external supervisors. The external supervisors will be requested to send a very brief report of the student to the

university mentor. The students who get jobs during the IV semester will be required to submit the documents

supplied by their employer (as per the company policy) as part of the internal evaluation. After the completion of

project work viva-voce will be conducted along with practical examinations by an external examiner appointed by

the Director, IBST.

M. Tech. Biotechnology

II Year: IV Semester

TECHNICAL COMMUNICATION MHU-1002/1001P

L T P C

2 0 0 2

Objectives:

On completion of the course students should be able to:

• To make them professionally skilled and employable in the present corporate set up

using their communication skills.

• To make them practice and demonstrate better language skills (listening, speaking,

reading and writing) in English.

• They will be able to demonstrate proficiency in communication and comprehension.

• They will be well versed in composing, drafting and editing résumé, report, proposal,

and research papers.

UNIT-I (10 Hours)

COMMUNICATION AND PRESENTATION STRATEGY

Communication: Process, Types, How to make it effective, Barriers to Communication

(interpersonal, intrapersonal, extra personal, cross-cultural), Body Language; Presentation

Strategy: Steps (planning, organization, preparation, and presentation), Types, Motives (general

and specific), Manner (Do’s and Don’ts), Methods (lecture, advertisement, paper presentation, PPT

presentations), and Art (how to make effective presentation)

UNIT-II (12 Hours)

TECHNICAL WRITING

CV and Business letters: CV drafting, Editing, Job application letter, Claim letter, Quotation letter,

Sales letter, Notice, Memo, Agenda and Minutes of Meeting; Proposal: Motives, Types (solicited

and unsolicited), Steps involved in Proposal Writing; Report: Types, Method of Writing, Various

Components; Technical Paper: Abstract, Various Sections (literature review, methodology,

analysis, interpretation, findings and recommendation), Steps involved in Technical Paper Writing,

Bibliography; Project and Dissertation: Motive, Components, Steps involved in Planning and

Drafting

UNIT-III (6 Hours)

TEXT BASED READING AND CRITICAL APPRECIATION

1. The Scientist by R.P. Singh

2. The Financial Expert by R. K. Narayana

Text Books

T1. Singh, R.P. “The Flea Market and Other Plays”, Authors Press

T2. Narayana, R.K. “The Financial Expert”

T3. Rizvi, M.A. “Effective Technical Communication”, Tata McGraw Hill

T4. Raman, M. and Sharma, S. “Technical Communication: Principles and Practice” Oxford

University Press.

Reference Books

R1. Sharma, R.C. and Krishna, M. “Business: Correspondence and Report Writing”, Tata

McGraw Hill, 3rd Edition

R2. Nitin, B. “Communicative English for Engineers and Professionals”, Pearson Education

India, 2010

R3. Budinski, K.G. “Engineers' Guide to Technical Writing”, ASM International, 2001

Course Learning Outcomes (CLO): On completion of this course, the students will be able to:

CLO Description Bloom’s

Taxonomy Level

CLO1 Utilize their communication skills to be professionally skilled and

employable.

3

Applying

CLO2 Demonstrate and build better language skills (listening speaking,

reading and writing) in English.

2,3

Understanding,

Applying

CLO3 Demonstrate proficiency in communication and comprehension. 2

Understanding

CLO4 Apply their skills in drafting and editing resume, report, proposal and

research paper.

3

Applying

Mapping of CLO’s with PLO’s

Course

Learning

Outcomes

Program Learning

Outcomes (PLOs)

Program Specific

Outcomes (PSOs)

PL

O1

PL

O2

PL

O3

PL

O4

PL

O5

PL

O6

PL

O7

PL

O8

PL

O9

PL

O10

PL

O11

PL

O12

PS

O1

PS

O2

PS

O3

PS

O4

CLO1 M M L L L M L M M H M H M L L

CLO2 M M L L L M L M M H M H H H M

CLO3 L M L L L M L M M H M M L L M

CLO4 M M L L L M L M M H M H M M M

H: High M: Medium L: Low

Bioprocess Engineering and Technology

MBT1011

L T P C

4 0 0 4

Course Learning Objectives:

• Enables thestudents to learn analysis and design of bioreactors for the

production of bioproducts.

• It exposes them to different modes of bioreactor operations.

• The students are given a comprehensive knowledge about fermentation

kinetics, design of ideal fermenters and also importance of mass transfer

effects in fermenters.

• Students will gain skills for applications in Biotechnology based industries.

UNIT I: Introduction to Bioprocess Engineering and engineering calculations

[8hours]

Bioprocess development an interdisciplinary challenge, engineering calculations:

recapitulation of basic concepts (Errors in Data Calculations, Presentation of

experimental data, data analysis, General procedures for plotting data, Process flow

diagrams)

UNIT II: Microbial growth and growth kinetics [8hours]

Microbial kinetics of growth, substrate utilization and product formation in batch,

chemostat and fed batch culture, Maintenance energy and yield concepts, Principles

of model building for biotechnological processes, unstructured models, structured

models.

UNIT III: Bioreactor design and control [8hours]

Design of various bioreactors: CSTR, fed batch systems, air-lift bioreactors,

fluidized bed bioreactors. Bioreactor design of agitator/agitator motor, power

consumption in aerated bioreactor, design of sparger, mixing time estimation,

oxygen mass transfer capability in bioreactor, Removal of Heat in bioreactor,

Main parameters to be monitored and controlled in fermentation processes,

Advanced control strategies: PID controllers, fuzzy logic based controllers and

artificial neural network based controllers.

UNIT IV: Modified reactor operations and scale-up [8hours]

Modifying batch and continuous reactions: Chemostat with recycle, multistage

chemostat systems and perfusion systems. Convective mass transfer: liquid-solid

mass transfer, liquid-liquid mass transfer, gas-liquid mass transfer, oxygen uptake in

cell cultures, oxygen transfer in fermenters, oxygen transfer inlarge vessels.

Scale-Up of Bioreactor: Scaling up procedure from laboratory to plant scale, scale

down.

UNIT V: Case studies in fermentation derived products

[8hours]

Case studies on Production of green chemicals, algal biofuels, recombinant Insulin.

Case studies should deal with medium design, reactor design & process optimization.

TEXTBOOKS:

1. Stanbury, P.F., Hall S. J. and Whitaker A. 2003. Principles of Fermentation

Technology. 2nd ed. Science & Technology Books.

2. Doran P. 2012. Bioprocess Engineering Pinciples, 2nd ed. Academic Press

3. Nielsen, J. and Villadsen, J. 2007. Bioreaction Engineering Principles. 2nd ed.

Springer science and business media.

REFERENCE BOOKS:

1. Bailey J. E. and Ollis D. F. 1986. Biochemical Engineering Fundamentals, 2nd

ed., McGraw Hill.

2. Shuler M. L. and Kargi F. 2002. Bioprocess Engineering: Basic Concepts. 2nd ed.

Prentice Hall Inc.

Course Learning Outcomes (CLO): On completion of this course, the students will

be able to

CLO Description

Bloom’s

Taxonomy

Level

CLO1 Understand how environmental conditions influence

cell growth and means to achieve optimal cell growth

in large scale.

2, 5

Understanding,

Evaluating

CLO2 Utilize the principles of Bioreactor Engineering for

designing and analysis of biological reactors for

industrially important primary and secondary

products.

3, 4

Applying,

Analyzing

CLO3 Gain an understanding of fermentation kinetics,

design of ideal fermenters andimportance of mass

transfer in fermenters.

2, 5

Understanding,

Evaluating

CLO4 Apply different mathematical formulas for

biocatalysis and for the bioreactor performance and

use those to plan and analyze bioprocesses.

3,

Applying

Mapping of CLOs with PLOs & PSOs

Course

Learning

Outcomes

Program Learning

Outcomes (PLOs)

Program Specific

Outcomes

(PSOs)

PL

O1

PL

O2

PL

O3

PL

O4

PL

O5

PL

O6

PL

O7

PL

O8

PL

O9

PL

O1

0

PL

O1

1

PL

O1

2

PS

O1

PS

O2

PS

O3

PS

O4

CLO1 M L M H L L M

CLO2 H M H H M M M

CLO3 M H M H M M M

CLO4 M M M M M M H M M M


Biochemistry and Metabolic regulation

MBT1012

L T P C

3 0 0 3


• This course covers the structure and function of bio molecules.

• This also focuses on metabolic pathways their coordinate regulation and

hormonal regulation.

• Through this course the students are exposed to importance of biological

macromolecules.

• They acquire knowledge in the quantitative and qualitative estimation of

biomolecules.

UNIT I: Structure and function of biomolecules [6hours]

Structure of carbohydrates, classification, homo and hetropolysaccharides, lipid

structure and function, classification of amino acid and its properties, protein,

glycoconjugates; glycoproteins, and glycolipid

UNIT II: Bioenergetics and general idea about Metabolism [6hours]

Laws of thermodynamics Biological energy and chemical transformations, coupled

Reactions, high energy phosphates compounds (ATP), Biological oxidation-

reduction reactions, Concept of convergent and divergent pathways

UNIT III: Carbohydrate metabolism and its regulation [6hours]

Carbohydrates- Glycolysis, (aerobic and anaerobic), glucoenogenesis and their

reciprocal regulation, fate of pyruvate, oxidative phosphorylation, pentose phosphate

pathway, reciprocal regulation of glycogen metabolism

UNIT IV: Nucleic acids and protein Metabolism and their regulation [7hours]

Purine and pyramidine metabolism and its regulation, Proteins Metabolism;

transamination and oxidative deamination reactions, role of pyridoxal phosphate in

transamination reaction, urea cycle, amino acids biosynthesis of non-essential amino

acids and its regulation

UNIT V: Lipid Metabolism and its regulation [5hours]

Lipids- Fatty acid synthesis, β-oxidation of fatty acids, Ketone bodies, Cholesterol

metabolism, regulation of lipid metabolism. Comparison of Lipid biosynthesis and

breakdown

TEXTBOOKS:

1. Lehninger, David. L. Nelson and Michael. M. Cox, 2004, Principles of

Biochemistry, 4th ed., Worth Publishers

2. Jeremy M. Berg, John L. Tymoczko and LubertStryer, 2004, Biochemistry, 5th

ed., Freeman Int. Edition

3. Erice Conn, Paul Stumpf, 2009, Outlines of Biochemistry, 5th ed., John Wiley &

Sons

REFERENCE BOOKS:

1. Donald Voet, Judith G. Voet, 2011, Biochemistry, 4th ed.,Wiley

2. Zubay G., 1993, Principal of Biochemistry, Brown (William C.) Co, U.S


be able to

CLO Description

Bloom’s

Taxonomy

Level

CLO1 Understand the structure of bio molecules and

identify their role in biological system.

2, 3

Understanding,

Applying

CLO2 Summarize the coordinate regulation of metabolic

pathways.

2

Understanding

CLO3 Compare the laws of thermodynamics and their

influence on metabolic pathways.

2, 5

Understanding,

Evaluating

CLO4 Examine the catabolic reactions of carbohydrates,

lipids and amino acids

4,

Analyzing


Course

Learning

Outcomes

Program Learning

Outcomes (PLOs)

Program Specific

Outcomes

(PSOs)

PL

O1

PL

O2

PL

O3

PL

O4

PL

O5

PL

O6

PL

O7

PL

O8

PL

O9

PL

O1

0

PL

O1

1

PL

O1

2

PS

O1

PS

O2

PS

O3

PS

O4

CLO1 M L M H L L M

CLO2 H M H H M M M

CLO3 H M M H H M M M

CLO4 M L M H H M H M M M


Computational and Structural Biology

MBT1013

Course Learning Objective:

• To provide foundation in fundamental concepts, tools and resources in

computational biology.

• To give an overview of biological sequence repositories and sequence

alignments.

• To provide information about protein sequence and structure bioinformatics.

• To introduce the concepts of in silico drug designing and importance of omics

technologies.

UNIT I: Introduction to computational biology [8hours]

Bioinformatics and its applications; sequence databases: NCBI, EMBL, DDBJ;

sequence submission and retrieval systems: Sequin, BankIT, DDBJ nucleotide

submission system; sequence file formats: FASTA, GCG, EMBL, MultiFASTA;

sequence alignment; local and global alignment; sequence alignment tools (Clustal

Omega, etc); BLAST: types and applications; organism specific databases: TAIR,

FlyBase, WormBase

UNIT II: Protein sequence and structure analysis [8hours]

Introductory protein structure: primary, secondary, tertiary and quaternary structure

of proteins; ExPASy Translate tool; protein motifs and domains; protein structure

visualization software: RasMol, PyMol, PDBViewer, Cn3D; tools and resources for

to understand relationship between sequence, structure and function of

biomolecules: RCSB/PDB database; SCOP and CATH for protein architecture;

overview of SWISS-MODEL Repository

UNIT III: Three-dimensional structure determination and prediction [8hours]

Macromolecular structure determination by X-ray crystallography and NMR;

protein structure prediction by comparative modelling approaches: homology

modelling, threading; ab initio structure prediction; online protein structure

prediction tools: Phyre2, I-TASSER, RaptorX, MODELLER; CASP (Critical

Assessment of protein Structure Prediction) experiment for protein structure

prediction.

L T P C

4 0 0 4

UNIT IV: Role of computational and structural biology in drug discovery

[8hours]

Drug discovery and development process; introduction to computer-aided drug

design; stages of in silico drug discovery; QSAR and drug design; protein

dockingand its applications; tools for docking: AutoDock and SwissDock; in silico

ADMET prediction; pharmacogenomics and personalized medicine

UNIT V: Omics technologies and big data [8hours]

Introduction to different omics technologies: genomics, transcriptomics, proteomics,

metabolomics; next-generation sequencing: Illumina platform and nanopore

sequencing; introduction to genome assembly; microarray technology for studying

the transcriptome; 2D-PAGE to understand the dynamic proteome; mass

spectrometry and peptide mass fingerprinting.

TEXTBOOKS:

1. Bujnicki, JM. 2006. Practical Bioinformatics. Springer

2. Ghosh, Z and Mallick, B. 2012. Bioinformatics: Principles and Applications.

Oxford University Press.

3. Krawetz, SA. 2008. Introduction to Bioinformatics: A Theoretical And Practical

Approach. Springer.

4. Mount, DW. 2004. Bioinformatics: Sequence and Genome Analysis. 2nded.Cold

Spring Harbor Laboratory Press.

5. Sundaralingam, R and Kumaresan, V. 2008. Bioinformatics. Saras Publication.

REFERENCE BOOKS:

1. Baxevanis, A and Ouellette, FBF. 2001. Bioinformatics: A Practical Guide to

the Analysis of Genes and Proteins. 2nd ed. John Wiley

2. Branden, C and Tooze, J. 2009. Introduction to Protein Structure. 2nd ed. Taylor

and Francis Group, Garland Science


be able to

CLO Description

Bloom’s

Taxonomy

Level

CLO1 Develop an understanding of how to make use of

biological databases to search for biological

sequences.

2, 3

Understanding,

Applying

CLO2 Utilize bioinformatics tools in protein structure

prediction.

3

Applying

CLO3 Understand the importance of computational

biology in drug designing.

2, 5

Understanding,

Evaluating

CLO4 Analyze how omics technologies are making it

possible to generate large amounts of biological data.

4,

Analyzing


Course

Learning

Outcomes

Program Learning

Outcomes (PLOs)

Program Specific

Outcomes

(PSOs) P

LO

1

PL

O2

PL

O3

PL

O4

PL

O5

PL

O6

PL

O7

PL

O8

PL

O9

PL

O1

0

PL

O1

1

PL

O1

2

PS

O1

PS

O2

PS

O3

PS

O4

CLO1 M L M H L L M

CLO2 H M H H M M M

CLO3 H M L H H M M M

CLO4 H M H M L M H M M M


Advanced Immunology and Immunological Techniques

MBT1014

Course Learning Objective:

• To teach immunology and immuno-technology to the students for their better

understanding of the immune system, types and mechanism of immunity,

immune responses, their tolerance and suppression.

• To introduce students with different tools and techniques involved in

diagnosis and identification of immune related diseases.

• To provide students with detail understanding of different cells of the immune

system and their role in immune protection and application of immunological

techniques.

• To gain knowledge about role of immune system in pathogenesis of infectious

diseases, cancer, autoimmune disease, AIDS.

UNIT I: Immunology fundamental concepts & anatomy of immune system

[8hours]

Components of innate and acquired immunity, phagocytosis, complement and

inflammatory responses, haematopoesis, organs and cells of the immune system-

primary and secondary lymphoid organs, lymphatic system, lymphocyte circulation,

lymphocyte homing, mucosal and cutaneous associated lymphoid tissue

(MALT&CALT), mucosal immunity, antigens- immunogens, haptens.

UNIT II: Cellular components of the immune system [8hours]

Immunoglobulins- basic structure, classes and subclasses of immunoglobulins,

antigenic determinants, multigene organization of immunoglobulin genes, B-cell

receptor, immunoglobulin superfamily, immunological basis of self –non-self

discrimination, kinetics of immune response, memory, B cell maturation, activation

and differentiation, generation of antibody diversity, T-cell maturation, activation

and differentiation and T-cell receptors, functional T Cell Subsets, cell-mediated

immune responses, ADCC, cytokines-properties, receptors and therapeutic uses.

UNIT III: Functioning of Immune system [8hours]

Major Histocompatibility Complex - MHC genes, MHC and immune responsiveness

and disease susceptibility, HLA typing, antigen processing and presentation-

endogenous antigens, exogenous antigens, non-peptide bacterial antigens and super-

L T P C

4 0 0 4

antigens, complement systems- mode of activation, classical and alternate pathways,

biological functions.

UNIT IV: Antigen-Antibody interactions [8hours]

Antigen antibody reactions in vitro methods, precipitation, agglutination,

compliment fixation, immunofluorescence and immune electrophoresis, RIA,

ELISA, in-vitro methods, skin tests and immune complex tissue demonstrations,

applications of these methods in diagnosis of microbial infections, flow cytometry

and immunoelectron microscopy.

UNIT V: Vaccinology [8hours]

Active and passive immunization, live, killed, attenuated, sub unit vaccines, vaccine

technology- role and properties of adjuvants, recombinant DNA and protein based

vaccines, plant-based vaccines, reverse vaccinology, peptide vaccines, conjugate

vaccines, antibody genes and antibody engineering- chimeric and hybrid monoclonal

antibodies, catalytic antibodies and generation of immunoglobulin gene libraries.

TEXTBOOKS:

1. Sudha, G and Shubhangi, S. 2013. Textbook of basic and clinical immunology.

1sted. India, University Press.

2. Khan, F. 2009. The elements of Immunology. 1sted. India, Pearson Education.

3. Goldsby, R., Kindt, T. and Osborne, A. 2002. Kuby Immunology. 6th ed.

Freeman.

REFERENCE BOOKS:

1. Brostoff, J., Seaddin, J., Male, D. and Roitt, I. 2002. Clinical Immunology. 6th

ed. Gower Medical Publishing.

2. Janeway, C.1999. Immunobiology. 4thed. Current Biology publications.

3. William, E. and Paul. 1999. Fundamental of Immunology, 4th ed. Lippencott

Raven.


be able to


Taxonomy Level

CLO1 Contrast and compare different components of

innate and acquired immunity.

2

Understanding,

CLO2 Demonstrate and identify cellular components of

immune systems.

2, 3

Understanding,

Applying

CLO3 Draw an inference between different immune/disease

developments.

4,

Analyzing

CLO4 Distinguish between receptors expressed on cells by

immunological techniques and compare with their

functions.

2, 4

Understanding,

Analyzing


Course

Learning

Outcomes

Program Learning

Outcomes (PLOs)

Program Specific

Outcomes

(PSOs)

PL

O1

PL

O2

PL

O3

PL

O4

PL

O5

PL

O6

PL

O7

PL

O8

PL

O9

PL

O1

0

PL

O1

1

PL

O1

2

PS

O1

PS

O2

PS

O3

PS

O4

CLO1 M L M H L L M

CLO2 H M H H M L M

CLO3 H M M H M M M

CLO4 M M M M M H L L M


BIOPROCESS ENGINEERING LAB

MBT1508


• To gain an overview of the Biotechnology industry and learn to appreciate the

integration of the processes used in the industry.

• To learn basic microbiology, molecular biology, and biochemistry laboratory

techniques within the context of the use of these techniques in industrial and

laboratory settings.

• To gain a comprehensive knowledge about fermentation kinetics.

• To gain knowledge of design of ideal fermenters and also importance of mass

transfer effects in fermenters.

List of experiments

• To perform Solid State fermentation (SSF) in production of enzymes from

metalo-tolerant amylolytic microbes.

• To perform Submerged State fermentation (SmF) in production of enzymes

from metalo-tolerant amylolytic microbes.

• To design a multistage/single downstream process for isolation of intracellular

products from a given microbial culture.

• To learn the technique of separation of biomolecules by the application of Gel

filtrations chromatography

• To compare the growth and death kinetics of industrially important microbes

produced by SSF and SmF.

• Production of natural antibiotics at lab scale from the microbes against

pathogens present in pond water.

• To Estimate the activity of purified alkaline amylase in free and immobilized

form with calcium alginate beads.

• Cell designer via spoken tutorial: Modeling tool for bioprocess optimization.

L T P C

0 0 2 1


be able to

CLO Description

Bloom’s

Taxonomy

Level

CLO1 Understand how environmental conditions influence

cell growth and means to achieve optimal cell growth

in the laboratory.

2, 5

Understanding,

Evaluating

CLO2 Utilize the principles of bioprocess for production and

analysis of industrially important primary and

secondary products.

3, 4

Applying,

Analyzing

CLO3 Understand the fermentation kinetics and

importance of downstream processing.

2, 5

Understanding,

Evaluating

CLO4 Apply different mathematical formulas for

biocatalysis and use those to plan and analyze

bioprocesses.

3,

Applying


Course

Learning

Outcomes

Program Learning

Outcomes (PLOs)

Program Specific

Outcomes

(PSOs)

PL

O1

PL

O2

PL

O3

PL

O4

PL

O5

PL

O6

PL

O7

PL

O8

PL

O9

PL

O1

0

PL

O1

1

PL

O1

2

PS

O1

PS

O2

PS

O3

PS

O4

CLO1 M L M H L L M

CLO2 H M H H M M M

CLO3 M H M H M M M

CLO4 M M M M M M H M M M


Biochemistry and Computational Biology Lab

MBT1509


• To use and develop bioinformatics programs for comparing &analyzing

biological sequence data to identify probable function.

• To provide foundation in fundamental concepts, tools and resources in

computational biology.

• To provide information about protein sequence and structure

bioinformatics.

• To learn methods used in biomolecules estimation.

List of experiments

• BLASTp of the protein sequences.

• Multiple sequence alignment using Clustal Omega.

• Prediction of protein structure using RasMol.

• Determination of function of a given protein and identify its orthologous

proteins.

• Estimation of total carbohydrates by Anthrone method.

• Estimation of the protein content of sample using Lowry’s / Bradford

method.

• Quantitative analysis of DNA using Diphenylamine (DPA) reaction.

• Biopython via Spoken Tutorial - Parsing given data using FASTA.

L T P C

0 0 2 1


be able to

CLO Description

Bloom’s

Taxonomy

Level

CLO1 Utilize basic algorithms used in Pairwise and Multiple

alignments for analysis of biological sequences.

3, 4

Applying,

Analyzing

CLO2 Understand the methodologies used for database

searching and determine the accuracy of database

search.

2, 5

Understanding,

Evaluating

CLO3 Determine the protein function from sequence

through analysis of data.

5

Evaluating

CLO4 Apply different laboratory test for analysis of

biological compounds.

3, 4

Applying,

Analyzing


Course

Learning

Outcomes

Program Learning

Outcomes (PLOs)

Program Specific

Outcomes

(PSOs)

PL

O1

PL

O2

PL

O3

PL

O4

PL

O5

PL

O6

PL

O7

PL

O8

PL

O9

PL

O1

0

PL

O1

1

PL

O1

2

PS

O1

PS

O2

PS

O3

PS

O4

CLO1 H L H H M L L

CLO2 H M L H H H M

CLO3 M H L H L L M

CLO4 M L H M M M H M M M


SEMINAR-1

MBT1504

L T P C

0 0 1 1


The objective of this course is

• To acquire the skills necessary to read and evaluate original research articles.

Most of the course will involve the discussion of current issues in the domain

of biotechnology.

• To encourage the students to study advanced engineering developments

• To prepare and present technical reports.

• To encourage the students to use various teaching aids such as overhead

projectors, power point presentation and demonstrative models.


be able to

CLO Description

Bloom’s

Taxonomy

Level

CLO1 Survey the changes in the technologies relevant to the

topic selected

4

Analyzing

CLO2 Discuss the technology and interpret the impact on

the society, environment and domain.

2, 5

Understanding,

Evaluating

CLO3 Compile report of the study and present to the

audience, following the ethics.

6

Creating

CLO4 Develop an understanding to review, prepare and

present technological developments.

2, 6

Understanding,

Creating


Course

Learning

Outcomes

Program Learning

Outcomes (PLOs)

Program Specific

Outcomes

(PSOs)

PL

O1

PL

O2

PL

O3

PL

O4

PL

O5

PL

O6

PL

O7

PL

O8

PL

O9

PL

O1

0

PL

O1

1

PL

O1

2

PS

O1

PS

O2

PS

O3

PS

O4

CLO1 H L H H M L L

CLO2 H M L H H H M

CLO3 M H L H L L M



S.

No. Subject


THEORY

1. MBT2009 Recombinant DNA Technology 4 0 - 40 60 100 4

2. MBT2010 Advance Down Stream

Processing 3 0 - 40 60 100 3

3. MBT2011 Molecular Biology and Virology 4 0 - 40 60 100 4

4. --- Department Elective – I 3 0 - 40 60 100 3

5. --- Department Elective – II 3 0 - 40 60 100 3


6. MBT2510 Recombinant DNA Technology

Lab 0 0 2 80 20 100 1

7. MBT2511 Molecular Biology and Virology

Lab 0 0 2 80 20 100 1

8. MBT2512 Comprehensive Viva Voce 0 0 0 80 20 100 1

9. MBT2504 Seminar-II 0 0 1 100 - 100 1

Total 17 0 5 540 360 900 21

M. Tech. Biotechnology I Year: II Semester

Recombinant DNA Technology

MBT2009

L T P C

4 0 0 4


• The course will provide knowledge of the advanced methods in recombinant DNA technology

• It provides an insight into various advances in recombinant DNA applications.

• A complete understanding of molecular techniques like DNA sequencing, restriction mapping.

UNIT I: Introduction to Bioprocess Engineering and engineering calculations [8 hours]

Milestones in genetic engineering, biosafety, issues–genetic engineering guidelines Molecular

Tools in Genetic Engineering – Restriction enzymes and DNA Modifying enzymes (Polymerases,

Reverse Transcriptase, Ligases, Alkaline phosphatase, Terminal deoxynucleotide transferases,

Nucleases - S1 nucleases etc.)Nucleic Acid isolation and purification yield analysis, Gel

electrophoresis, DNA and RNA markers. Restriction mapping of DNA fragments and Map

construction, nucleic acid amplification (PCR analysis) and its applications, Real time PCR.

UNIT II: Gene cloning strategies [9 hours]

Gene cloning vectors (Plasmids, bacteriophages, cosmids, phagemids, Artificial chromosomes),

gene cloning strategies, transformation and selection of recombinants; Construction of DNA

libraries (Genomic library and cDNA library preparations–mRNA enrichment, reverse

transcription, use of linkers and adaptors); and their screening; Alternative strategies of Gene

cloning; Cloning of differentially expressed genes. Site directed Mutagenesis and Protein

Engineering.

UNIT III: Gene expression [9 hours]

Study of introduced Gene expression – hybridization techniques, Northern blot analysis, Primer

extension, S1 mapping, RNase protection assays, Reporter assays), Nucleic acid microarrays. Gene

expression in bacteria and Yeast, expression in insects and insect cells, expression in mammalian

cells, expression in plants – characterization of recombinant proteins, stabilization of proteins;

Phage display, Yeast Two- and three Hybrid system.

UNIT IV: Transgenic technology [7 hours]

Gene tagging (T-DNA tagging and transposon tagging) in gene analysis (identification and

isolation of gene), transgenic and gene knockouts technologies-targeted gene replacement,

chromosome engineering, gene silencing, transgenic plants.

UNIT V: Applications[7 hours]

Genetic diseases-Detection and Diagnosis, Gene therapy – ex vivo, in vivo, gene delivery systems,

viral and non-viral, DNA marker technology in plants, DNA fingerprinting, Genetically engineered

biotherapeutics and vaccines and their manufacturing, Transgenic animals and Bio-pharming.

TEXT BOOKS:

1. SmitaRastogi and Neelam Pathak, 2009.Genetic Engineering by First edition, Oxford

University Press, .

2. T. A. Brown, 2010, Gene cloning and DNA Analysis by, Sixth edition, Wiley-Blackwell.

REFERENCE BOOKS:

S.B. Primrose and R.M. Twyman, 2006, Principles of Gene Manipulation and Genomics by

Seventh Ed, Blackwell publishing.


be able to:


Taxonomy Level

CLO1 Apply techniques of genetic engineering in living organisms for human benefit in medicine, agriculture and industry.

3, 1

Applying,

Remembering,

Understanding

CLO2 Illustrate and make use of the advancements of recombinant DNA technology.

2, 3

Understanding,

Applying,

CLO3 Understand the methods employed in rDNA technology and

demonstrate practical experience of selected Molecular

Biological Techniques

2

Understanding

CLO4 Develop new technology for betterment and expansion of

Biotechnology.

3

Applying


Course

Learning

Outcomes

Program Learning

Outcomes (PLOs)

Program Specific

Outcomes(PSOs) P

LO

1

PL

O2

PL

O3

PL

O4

PL

O5

PL

O6

PL

O7

PL

O8

PL

O9

PL

O1

0

PL

O1

1

PL

O1

2

PS

O1

PS

O2

PS

O3

PS

O4

CLO1 H M M H

H M M M

CLO2 M H H L

H M M M

CLO3 M L

H M M M

CLO4 H L M

M M M M


RECOMBINANT DNA TECHNOLOGY LAB

MBT2510

L T P C

0 0 2 1


• The Lab course will provide knowledge of the advanced technique in recombinant DNA

technology

• It provides an insight into various advance tools used in recombinant DNA technology lab.

• It will help student become familiar with use of restriction enzymes, PCR steps, competent

cells preparation.

List of experiments

• Plasmid DNA isolation from bacteria.

• Separation of DNA samples by Agarose gel electrophoresis.

• Preparation of competent cells for transformation.

• Restriction digestion of DNA using restriction enzymes.

• Bacterial transformation.

• Amplification of DNA by Polymerase Chain Reaction (PCR).

• Perform DNA ligation.

Course Learning Outcomes (CLO): On completion of this course, the students will be

able to:


Taxonomy Level

CLO1 Apply techniques of genetic engineering in living organisms like bacteria.

3, 1

Applying,

Remembering,

Understanding

CLO2 Illustrate and make use of the advancements of recombinant DNA technology techniques.

2, 3

Understanding,

Applying,

CLO3 Understand the methods and protocols employed in rDNA

technology and demonstrate practical experience of selected

Molecular Biological Techniques

2

Understanding

CLO4 Develop new protocols associated with molecular biology lab

for its betterment. 3

Applying


Course

Learning

Outcomes

Program Learning

Outcomes (PLOs)

Program Specific

Outcomes(PSOs) P

LO

1

PL

O2

PL

O3

PL

O4

PL

O5

PL

O6

PL

O7

PL

O8

PL

O9

PL

O1

0

PL

O1

1

PL

O1

2

PS

O1

PS

O2

PS

O3

PS

O4

CLO1 H M L M H

H M M M

CLO2 L M L H H L

H M M M

CLO3 L M L M L

H M M M

CLO4 H M L L M

M M M M


Advanced Downstream Processing

MBT2010

L T P C

4 0 0 4


• The syllabus will help the students to be familiar with various steps of

downstream processing from product extractions to product purifications.

• Students will gain knowledge, about the role of Downstream Processing in

Bio-separation and Biotechnology.

• To learn techniques of insoluble removal and predict operating optimal

parameters for large scale operations with effective strategies of downstream

processing based on characteristics of bio-molecules.

• To learn the various techniques of cell disruption, product preservations,

packaging and shelf life.

UNIT I: Scope of downstream processing [7 hours]

Scope of Downstream Processing: Importance of Down Stream Processing (DSP) in

biotechnology, characteristics of products, criteria for selection of bio-separation

techniques. Role of DSP methods in bioprocess economics. Methods for cell

disruption: Cell disruption methods types, why there is need for cell disruption

(Homogenizer, French press &Dynomill) for intracellular products, cell disruption

equipment. Applications in bio-processing. Flocculation: Principles, flocculating

agents, and its applications. Coagulation: Principles and its applications in bio-

processing.

UNIT II: Solid liquid separation [7 hours]

Principles filter aids, constant volume and pressure filtration, specific cake

resistance, equivalent cake thickness, filtration equipment’s viz; plate and frame

filter press, vacuum filters, leaf filters. Sedimentation: Principles and types (Batch

sedimentation viz; thickener). Centrifugation: Principles, centrifuge effect, g-

number, sigma factor, centrifuges types (viz; basket centrifuge, tabular centrifuge,

disc-bowl)

UNIT III: Extraction of value added products [7 hours]

Adsorption: principles, equipment and its applications in bio-processing.

Precipitation: Principles, equipment and applications in bio-processing. Foaming:

Principles, various foaming agents and their interaction with the products,

applications in bioprocess. Liquid-liquid Extraction; Extraction process and

principles, phase equilibrium and distribution, batch and continuous extraction, co-

current and counter current extraction processes, L-L-equipment. Applications in

bio-technology.

UNIT IV: Membrane separation processes [7 hours]

Basic principles of membrane separation, membrane characteristics, different types

of membranes, criteria for selection of membranes. Chromatographic separation and

Electrophoresis Methods: Principles of chromatographic separation methods,

different types of chromatographic methods, with applications in bio-processing.

Principles of electrophoresis, SDS- PAGE, 2D gel electrophoresis, capillary

electrophoresis.

UNIT V: Product polishing and packaging[7 hours]

Evaporation: Theory of evaporation, single effect and multiple effect evaporation,

steam economy, efficiency of evaporators, various evaporation equipment.

Crystallization: Principles of crystallization, crystallization equipment. Applications

in bio-processing. Drying: Various types of drying methods, principles of drying,

drying curves, various types of industrial dryers and their criteria for choice. Freeze

drying technique and its advantages over other methods. Applications in bio-

processing.

TEXTBOOKS:




3. Nielsen, J. and Villadsen, J. 2007. Bioreaction Engineering Principles. 2nd ed.

Springer science and business media.

REFERENCE BOOKS:

1. Bailey J. E. and Ollis D. F. 1986. Biochemical Engineering Fundamentals, 2nd ed.,

McGraw Hill.

2. Shuler M. L. and Kargi F. 2002. Bioprocess Engineering: Basic Concepts. 2nd ed.

Prentice Hall Inc.


able to:


Taxonomy Level

CLO1 Distinguish, identify and explain between different

separation techniques and design a combination of

downstream techniques for a given process.

4, 3, 2, 6

Analyzing,

Applying,

Remembering,

Understanding

Creating

CLO2 Analyze scientific results from real examples and

estimate operating parameters for a particular

operation.

4, 6

Analyzing,

Creating

CLO3 Demonstrate how polishing, packaging and

preserving of purified product is important.

2

Understanding

CLO4 Create protocols and procedure for purification.

6

Creating


Course

Learning

Outcomes

Program Learning

Outcomes (PLOs)

Program Specific

Outcomes(PSOs) P

LO

1

PL

O2

PL

O3

PL

O4

PL

O5

PL

O6

PL

O7

PL

O8

PL

O9

PL

O1

0

PL

O1

1

PL

O1

2

PS

O1

PS

O2

PS

O3

PS

O4

CLO1 H L L M

H M M M

CLO2 M L M H

H M M M

CLO3 M H M

H M M M

CLO4 M M M M M M


Molecular Biology and Virology

MBT2011

L T P C

4 0 0 4


• This course will expose students to the genome organization, and chromosome

structure.

• It will explain detailed mechanisms involved in the replication and regulation

of expression of genetic information for prokaryotes and eukaryotes.

• The course will familiarize students with signal transduction pathways

operating in the cell. In addition, students will also be introduced to the basics

of virology.

UNIT I: Chromosome structure, DNA replication, repair and recombination

[8 Hours]

Eukaryotic chromatin and chromosome organization (nucleosome solenoid model);

structure of DNA; gene organization; repetitive DNA; CpG islands; DNA

replication; DNA recombination and repair; mobile genetic elements (transposons

and retrotransposons)

UNIT II:Gene expression in prokaryotes and eukaryotes [8 Hours]

Types and structure of RNA; cis-regulatory elements; transcription factors;

transcription in bacterial versus eukaryotic cells; splicing; gene expression regulation

in prokaryotes and eukaryotes

UNIT III:Protein synthesis, localization and degradation [8 Hours]

Protein synthesis machinery (rRNA, tRNA and ribosomes); genetic code;

mechanism of translation; posttranslational modifications; protein folding; protein

localization; protein degradation

UNIT IV: Signal transduction [8 Hours]

Fundamentals of cell signaling; cell receptors; first messengers (hormones,

neurotransmitters and cytokines); second messengers (cAMP, cGMP, inositol

trisphosphate, diacylglycerol and calcium); signaling pathways (cAMP-dependent

pathway; Ras/MAPK pathways; JAK-STAT pathway; cAMP and activation of

CREB)

UNIT V: Extraordinary diversity of viruses [8 Hours]

Basics of virus structure; classification of viruses; bacteriophages (lysogenic and

lytic cycles); plant viruses (tobacco mosaic virus, cauliflower mosaic virus); animal

viruses (adenovirus, SV40, hepatitis B virus and HIV); prions

TEXT BOOKS:

1. Lodish H, Berk A, Zipursky SL, Matsudaira P, Baltimore D, and Darnell J.

Molecular Cell Biology. 6th ed. W. H. Freeman Company

2. Benjamin, L. 2007 Gene IX, 9th Edition, Jones and Barlett Publishers

REFERENCE BOOKS:

1. Watson JD, Baker TA, Bell SP, Gann A, Levine M, Losick R. 2014.

MolecularBiology of the Gene, 7th ed. Pearson education, USA


able to:


Taxonomy Level

CLO1 Understand the principles of molecular biology and

virology.

2

Understanding

CLO2 Gain and combine in-depth knowledge of DNA replication,

repair and recombination.

6,

Creating

CLO3 Understanding of molecular processes that occur in cells

during transcription and translation will help him/her to

improve and maximize his/her health and safety

consideration

2, 6,

Understanding,

Creating

CLO4 Translate knowledge of major cell signaling pathways, and

their insight into the structure and types of viruses will help

them to determine right strategies for generating solution

based approach.

2, 5, 6

Understanding,

Evaluating,

Creating


Course

Learning

Outcomes

Program Learning

Outcomes (PLOs)

Program Specific

Outcomes(PSOs)

PL

O1

PL

O2

PL

O3

PL

O4

PL

O5

PL

O6

PL

O7

PL

O8

PL

O9

PL

O1

0

PL

O1

1

PL

O1

2

PS

O1

PS

O2

PS

O3

PS

O4

CLO1 H L H

H M M M

CLO2 M M L L H M M M

CLO3 M M

H M M M

CLO4 M M L

L H M M


Molecular Biology and Virology Lab

MBT2511

L T P C

0 0 2 1


• The Lab course will provide knowledge of the advanced technique in molecular biology and

virology.

• It provides an insight into various advance tools used in chromosomal DNA isolation, plasmid

DNA isolation, cDNA synthesis and restriction mapping technology.

• It will help student become familiar with use of PCR amplification, restriction mapping,

enzymes, PCR steps, DNA isolation by CTAB method and RNA isolation from plants by

Trizol®.

List of experiments

• DNA isolation from plants by CTAB method

• Chromosomal DNA isolation from E. coli

• Plasmid DNA isolation from E. coli

• RNA isolation from plants by Trizol® reagent method

• cDNA synthesis from plant RNA

• Restriction mapping of lambda phage DNA by BamHI and EcoRI

• PCR amplification of selected genes


be able to:


Taxonomy Level

CLO1 Apply techniques of molecular biology and virology. 3, 1

Applying,

Remembering,

Understanding

CLO2 Illustrate and make use of the advancements of DNA isolation from plants by CTAB method.

2, 3

Understanding,

Applying,

CLO3 Understand the methods and protocols employed in

molecular biology and virology lab.

2

Understanding

CLO4 Develop new protocols associated with molecular biology and

virology lab for its betterment. 3

Applying


Course

Learning

Outcomes

Program Learning

Outcomes (PLOs)

Program Specific

Outcomes(PSOs) P

LO

1

PL

O2

PL

O3

PL

O4

PL

O5

PL

O6

PL

O7

PL

O8

PL

O9

PL

O1

0

PL

O1

1

PL

O1

2

PS

O1

PS

O2

PS

O3

PS

O4

CLO1 H M L L M M H

H M M M

CLO2 L M L L H M H L

H M M M

CLO3 L M L L L H M L L

H M M M

CLO4 H M L M L M M

M M M M


Nanobiotechnology and Nanodevices

MBT2105

L T P C

3 0 0 3


• The course aims at introducing the underlying principles and applications in the

emerging field of nano-biotechnology.

• The course provides relevant information and examples to give students a sound

knowledge of the tools and principles relevant at the nanoscale dimension.

• The syllabus is made with objectivity of making student abreast with nano

devices like biosensors.

• The course is aimed to provide modern applications of nano drug delivery.

UNIT I:Introduction [6 Hours]

Definition of Nanotechnology; history of nanotechnology; nanotechnology to

nanobiotechnology; significance of nano-size and surface-volume ratio; bottom-up

and top-down methods of synthesis; self-assembly; Crane’s principle;

nanolithography.

UNIT II:Basic Characterization Techniques[6 Hours]

Electron microscopy: scanning electron microscopy and transmission electron

microscopy; atomic force microscopy; photon correlation spectroscopy; fourier

transform infrared spectroscopy; X-Ray diffraction; circular dichorism

UNIT III: Nanostructures and Nanosystems[8 Hours]

Carbon nanotubes; fullerenes; nanowires; metallic nanoparticles; dendrimers;

quantum dots; ultrasound contrast agents; nanoarrays; DNA computers; DNA motors;

nanopore technology, biogenic nanoparticles; stealth nanoparticles; virus like

nanoparticles; application of nanostructures in diagnostics and forensic science

https://www.google.co.in/url?sa=t&rct=j&q=&esrc=s&source=web&cd=3&cad=rja&uact=8&ved=0ahUKEwjn79v--crRAhXDwI8KHebhBLYQFggwMAI&url=https%3A%2F%2Fen.wikipedia.org%2Fwiki%2FFourier_transform_infrared_spectroscopy&usg=AFQjCNEkGKrQDSUqBI8YwA1tBD3C6DnsUQ&sig2=_qkdr2aXjWuiJetonxALiA

https://www.google.co.in/url?sa=t&rct=j&q=&esrc=s&source=web&cd=3&cad=rja&uact=8&ved=0ahUKEwjn79v--crRAhXDwI8KHebhBLYQFggwMAI&url=https%3A%2F%2Fen.wikipedia.org%2Fwiki%2FFourier_transform_infrared_spectroscopy&usg=AFQjCNEkGKrQDSUqBI8YwA1tBD3C6DnsUQ&sig2=_qkdr2aXjWuiJetonxALiA

UNIT IV:Nano Drug Delivery[8 Hours]

Conventional drug delivery; drug delivery vehicles; delivery profiles; targeted drug

delivery; active targeting; physical and chemical targeting; ultrasound based targeting;

smart drug delivery system; modern applications of nano drug delivery

UNIT V:Biosensors[7 Hours]

Introduction and basic characteristics of biosensors; types of biosensors: piezo electric

sensors; optical sensors; calorimetric sensors; electrochemical sensors; gold

nanoparticles as biosensors; future prospective and challenges; application of

biosensors in disease diagnosis and environmental monitoring

TEXTBOOKS:

1. Goodsell DS. 2004. Bionanotechnology: Lessons from Nature, 1st ed. Wiley-Liss

2. Papazoglou ES and Parthasarathy A. 2007. BioNanotechnology. Morgan &

Claypool.

REFERENCE BOOKS:

1. Niemeyer CM and Mirkin CA. 2007. Nanobiotechnology- Concepts, Applications

and Perspectives. Wiley-VCH.

2. Malsch NH. 2005. Biomedical Nanotechnolog. 1st ed. CRC Press.


be able to:


Taxonomy Level

CLO1 Demonstrate a working knowledge of nanotechnology

principles and applications.

2

Understanding

CLO2 Make use of the nanotechnology based tools for disease

diagnosis and environmental monitoring.

3

Applying

CLO3 Explain the nanoscale paradigm in terms of properties at

the nanoscale dimension.

2

Understanding

CLO4 Identify career paths and requisite knowledge and skills for

career in nano-biotechnology.

3

Applying


Course

Learning

Outcomes

Program Learning

Outcomes (PLOs)

Program Specific

Outcomes(PSOs) P

LO

1

PL

O2

PL

O3

PL

O4

PL

O5

PL

O6

PL

O7

PL

O8

PL

O9

PL

O1

0

PL

O1

1

PL

O1

2

PS

O1

PS

O2

PS

O3

PS

O4

CLO1 H L M

H M M M

CLO2 L L L

H M M M

CLO3 H M

H M M M

CLO4 L L H

M M M M


Microbial Engineering

MBT 2106

L T P C

3 0 0 3


• The objective of this course is to familiarize the students with the fundamentals of material

balance, energy balance, media optimization, sterilization techniques and various models

of microbial growth.

• Further this course deals with the use of various diverse microbes in various domains such

as bioleaching, production of biodiesel, biofertilizers, biosensors and fuel cells.

• Course has focused on the industrial applicability of the microbiology.

• Course will enhance knowledge on methods used for optimizing medium.

UNIT I: [7 Hours]

Introduction to Microbial Engineering and Material Balance

Introduction to industrial microbial processes and products; Material Balance: thermodynamic

preliminaries; law of conservation of mass; types of material balance and calculations; material

balances with recycle, by-pass and purge streams; growth stoichiometry and elemental balances;

electron balances; biomass yield; product stoichiometry; theoretical oxygen demand; maximum

possible yield.

UNIT II: [7 Hours]

Energy Balance

Basic energy concepts; intensive and extensive properties; enthalpy; general energy-balance

equations; enthalpy calculation procedures; enthalpy change in non-reactive processes; steam

tables; energy-balance calculations without reaction; enthalpy change due to reaction; heat of

combustion; heat of reaction at non-standard conditions.

UNIT III: [7 Hours]

Media Optimization and Sterilization

Medium optimization techniques: Plackett-Burman design; response surface optimization

technique by one factor at a time; response surfaces in two dimensions.

Sterilization: medium sterilization; kinetics of thermal death of cells and spores; design of batch

and continuous thermal sterilization; filter sterilization of air and media.

UNIT IV: [7 Hours]

Structured and Unstructured Models for Microbial Growth

Kinetics of balanced growth: Monod growth kinetics; other form of growth kinetics: Moser

Equation, Tessier Model, Contois model, Malthus law, Logistic model; Kinetics based on

molecular mechanisms; compartment models; Metabolic models.

UNIT V: [7 Hours]

Modern Trends in Microbial Engineering Applications

Application of microbial engineering in: biosensors; fuel cells; chemical degradation; biomining

and bioleaching of ores; biodiesel production; bioremediation.

TEXT BOOKS

1. Stanbury, P.F., Hall S. J. and Whitaker A. 2003. Principles of Fermentation Technology. 2nd ed. Science & Technology Books.


3. Nielsen, J. and Villadsen, J. 2007. Bioreaction Engineering Principles. 2nd ed. Springer science

and business media.

REFERENCE BOOKS

1. Bailey J. E. and Ollis D. F. 1986. Biochemical Engineering Fundamentals, 2nd ed., McGraw

Hill.

2. Schugerl K. and Bellgart K.H. (Eds). 2000. Bioreaction engineering, Modeling and control.

Springer-verlog, Berlin.


able to:


Taxonomy

Level

CLO1 Tell about various media optimization techniques and sterilization.

1

Remembering

CLO2 Apply material and energy balance for process engineering. For

production of biodiesel, biofertilizers etc. using microbes.

3

Applying

CLO3 Distinguish various models for microbial growth, which find

applications in industrial microbes based processes.

4

Analyzing

CLO4 Discuss about the modern trends in microbial engineering with their

applications. 6

Creating


Course

Learning

Outcomes

Program Learning

Outcomes (PLOs)

Program Specific

Outcomes (PSOs) P

LO

1

PL

O2

PL

O3

PL

O4

PL

O5

PL

O6

PL

O7

PL

O8

PL

O9

PL

O1

0

PL

O1

1

PL

O1

2

PS

O1

PS

O2

PS

O3

PS

O4

CLO1 H M H H M M L

CLO2 M M M L M H L H M M L

CLO3 M H M L H M M L

CLO4 L L L L L L M M M L


Biocatalysis and Enzyme Reaction

MBT 2202

L T P C

3 0 0 3


• The major objective of this course is to acquaint students with fundamental of biocatalysis,

their characterization and applications in various areas.

• The objective of the course is to provide a deeper insight into the fundamentals of enzyme

structure and function of soluble and immobilized enzymes.

• To know the current applications and future potential of enzymes.

• To understand the enzyme kinetics.

UNIT I: Biocatalysis [7 Hours]

Current Status, Advantages & disadvantages, Comparison with other Catalysts,

Biocatalysts as a technology, Green Chemistry.

UNIT II: Characterization of a biocatalyst [7 Hours]

Enzyme Kinetics, Basis of Enzyme Action, Theories of Enzyme Catalysis,

Efficiency, Stability, Selectivity of Enzymes, Screening of New Enzyme Activity.

UNIT III:Biocatalytic asymmetric synhthesis [7 Hours]

Basic of stereochemistry; Enantiomerically pure amino acids, Hydroxy esters with

carbonyl reductase, Alcohols with ADH, Penicillin G, Ephedrine, Chiral drugs,

Anticholesterol drugs, Anti-infectives, Anti-AIDS drugs, Cardiovascular drugs,

Applications of Lipases and Esterases in the Pharma industry, Steroids.

UNIT IV: Biocatalysis in non-conventional media [7 Hours]

Enzymes in organic solvents, Advantages of Biocatalysis in organic media, Role of

water in Enzyme reactions in Organic solvents, Substrate as solvent, Ionic liquids

and Supercritical Solvents for enzymatic reactions.

UNIT V: Industrial enzymes [7 Hours]

Enzymes in the food industry, Cell-wall degrading enzymes, Lipases, Proteases,

Amylases, Xylanases, Enzymes in brewing, Fat splitting, Enzymes in the paper and

pulp industry, Enzymes in the textile industry, Enzymes for preservation, The future

of enzyme applications..

TEXT BOOKS:

1. Biocatalysis: Fundamentals & Applications by Andreas Sebastian Bommarius ,

Bettina R. Riebel, VCH.

2. Biotransformations in Organic Chemistry by Kurt Faber, Springer Berlin.

REFERENCE BOOKS:

1. Enzymes by palmer, Enzymes in Industry by Wolfgang Aehle, Wiley-VCH.


able to:


Taxonomy

Level

CLO1 Gain knowledge and demonstrate the characterization, synthesis

and applications of enzymes in food, brewing, textile and various

other industries.

2

Understanding

CLO2 Make use of ability of the biocatalysts in society, industries and

environment. 3

Applying

CLO3 Explain structure, functions and the mechanisms of action of

enzymes.

2

Understanding

CLO4 Learn kinetics of enzyme catalyzed reactions and identify enzyme

inhibitory and regulatory process. And will be able to perform

immobilization of enzymes.

3

Applying


Course

Learning

Outcomes

Program Learning

Outcomes (PLOs)

Program Specific

Outcomes(PSOs) P

LO

1

PL

O2

PL

O3

PL

O4

PL

O5

PL

O6

PL

O7

PL

O8

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O9

PL

O1

0

PL

O1

1

PL

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2

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O1

PS

O2

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O3

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CLO1 H L L M

H M M M

CLO2 M L M H

H M M M

CLO3 M H M

H M M M

CLO4 M M M M M M

M M M M


Animal Cell Science and Technology

MBT2203

L T P C

3 0 0 3


• This course aims to impart in students an understanding of the primary cell culture and

methods that convert them to long term established cultures.

• They will be exposed to all the factors which could impact cell culture and equipment

requirements for propagation.

• Awareness is generated about recent advances in the area of stem cell technology, organ

culture, tissue engineering etc.

• Course will enhance knowledge in applications of animal cell culture technology.

UNIT I: [7 Hours]

Basics of Animal Cell and Its Culturing

Structure and organization of an animal cell, Types of animal cell culture – cell culture,

organ/tissue culture, organotypic culture and histotypic culture, Equipments and materials needed

for animal cell culture technology.

UNIT II: [8 Hours]

Animal Cell Culture Medium and Its Components and Their Significance

Introduction to the balanced salt solutions and growth medium, Brief discussion on the chemical,

physical and metabolic functions of different constituents of culture medium, Role of carbon-di-

oxide and role of serum and its supplements in maintaining cells in culture medium, Serum and

protein free defined media and their application.

UNIT III: [7 Hours]

Basic Techniques of Mammalian Cell Culture in vitro

Primary and established cell lines, Biology and characterization of the cultured cells, measuring

parameters of growth. Maintenance of cell culture, Cell separation, Cell transformation, Cell

synchronization, Measurement of viability and cytotoxicity, Apoptosis – characteristic features and

molecular mechanisms, Measurement of cell death.

UNIT IV: [7 Hours]

Engineering Animal Cells

Somatic cell genetics, Cell culture based vaccines, Genetic engineering of mammalian cells in

culture, Scaling up of animal cell culture, Stem cell cultures – embryonic and adult stem cells and

their applications.

UNIT-V: [6 Hours]

Applications of Animal Cell Culture

Three dimensional culture and tissue engineering, Applications of animal cell culture technology

(heterologous, Primary culture/CEF culturing, Protein Expression).

TEXT BOOKS

1. Culture of Animal Cells, Fl. Ian Froshney. Wiley-Liss.

2. Animal Cell Culture - Practical Approach, Ed. John R.W. Masters, OXFORD

REFERENCE BOOKS

1. Cell Growth and Division: A Practical Approach. Ed. R. Basega, IRL Press.

2. Cell Culture Lab Fax. Eds. M Butler & M. Dawson, Bios Scientific Publications Ltd. Oxford.


able to:


Taxonomy

Level

CLO1 Demonstrate the ability for development of primary established cell

culture.

2

Understanding

CLO2 Develop awareness in interlinking of different fields for the

development of biological organs.

3

Applying

CLO3 Explain the methods used in maintenance of cell culture, Cell

separation, Cell transformation, Cell synchronization and Measurement

of viability and cytotoxicity of cell.

5

Evaluating

CLO4 Discuss application of biotechnological techniques in animal

biotechnology.

6

Creating


Course

Learning

Outcomes

Program Learning

Outcomes (PLOs)

Program Specific

Outcomes (PSOs) P

LO

1

PL

O2

PL

O3

PL

O4

PL

O5

PL

O6

PL

O7

PL

O8

PL

O9

PL

O1

0

PL

O1

1

PL

O1

2

PS

O1

PS

O2

PS

O3

PS

O4

CLO1 H L H M H H M L

CLO2 H M L M H H H H M H

CLO3 H M H M L H M M L

CLO4 L L H M M M L


Cell and Tissue Engineering

MBT2204

L T P C

3 0 0 3


• The course aims at introducing the underlying principles and applications related to tissue

engineering and regenerative medicine.

• The course will also cover the application of engineering principles, combined with

molecular cell biology, to develop fundamental understanding of property-function

relationships in cells and tissues.

• Exploitation of this understanding to manipulate cell and tissue properties rationally to

alter, restore, maintain, or improve cell and tissue functions as well as to design bio artificial

tissue substitutes.

• The course will enhance knowledge on tissue organization, its components and functions.

UNIT I: [7 Hours]

Introduction of Cell and Tissue Engineering

Definition; cells as therapeutic agents with examples; cell numbers and growth rates; measurement

of cell characteristics: cell morphology, cell number and viability, cell-fate processes, cell motility,

cell function.

UNIT II: [7 Hours]

Tissue Biology

Tissue organization; tissue components; tissue types; functional subunits; tissue dynamics;

homeostasis in highly prolific tissues and tissue repair; angiogenesis; cell-matrix and cell-cell

interactions.

UNIT III: [7 Hours]

Stem Cells Biology

Introduction; types and sources of stem cell with characteristics; potency and plasticity of stem

cells; sources; embryonic stem cells; hematopoietic and mesenchymal stem cells; stem cell

markers; FACS analysis; cancer stem cells; induced pleuripotent stem cells.

UNIT IV: [7 Hours]

Biomaterials in Tissue Engineering

Properties of biomaterials: surface, bulk, mechanical and biological properties; scaffolds

preparation and tissue engineering; types of biomaterials; applications of biomaterials;

modifications of biomaterials.

UNIT V: [7 Hours]

Clinical Applications

Stem cell therapy; tissue engineering therapies; in-vitro organogenesis; gene therapy; product

characterization; safety; efficacy; preservation–freezing and drying; ethical issues

TEXT BOOKS

1. Palsson BO and Bhatia SN. 2009. Tissue Engineering. Pearson Publishers

2. Gorodetsky R and Schäfer R. 2011. Stem cell based tissue repair. Cambridge: RSC Publishing

REFERENCE BOOKS

1. Obradović B. 2012. Cell and Tissue Engineering. Springer

2. Lanza R, Langer R and Vacanti J. 2007. Principles of Tissue Engineering. 1st ed. Elsevier Inc.


able to:


Taxonomy

Level

CLO1 Relate the basic knowledge of tissue engineering, stem cell and their

role in clinical research

1

Remembering

CLO2 Identify career paths and requisite knowledge and skills for career in

tissue engineering.

3

Applying

CLO3 Analyze structural, functional and comparative genomics of farm

animals and its application for livestock improvement.

4

Analyzing

CLO4 Discuss about properties of biomaterials and their applications. 6

Creating


Course

Learning

Outcomes

Program Learning

Outcomes (PLOs)

Program Specific

Outcomes (PSOs) P

LO

1

PL

O2

PL

O3

PL

O4

PL

O5

PL

O6

PL

O7

PL

O8

PL

O9

PL

O1

0

PL

O1

1

PL

O1

2

PS

O1

PS

O2

PS

O3

PS

O4

CLO1 H L H L H M M L

CLO2 M M L M H L H M M H

CLO3 M M H M M H M M L

CLO4 M M M L H M M M L


COMPREHENSIVE VIVA

MBT 2512

L T P C

0 0 0 1


• The students will get better understanding of research papers and technical communication.

• And will develop an understanding about utilization of research papers, short

communications and thesis published by researcher from reputed university for developing

more knowledge.

• Students will present the literature review and develop their understanding about the specific

area of their interests.

• The students will be able to have educational opportunities that will enhance their learning

and thinking capabilities.

Learning Outcomes: At the end of this course the student is able to:

CLO Description Bloom’s Taxonomy

Level

CLO1 Develop an understanding to review, prepare and present

technological developments. Will enhance their

communication skills and develop strong command on the

subject.

2, 6

Understanding,

Creating

CLO2 Build an understanding about the recent advances in the field

and subject of their choice related to biotechnology and

biosciences.

2, 3

Understanding

Applying

CLO3 Analyse the new development in techniques and concepts. 4

Analyzing

CLO4 Adapt the skills of technical writing and communication. 6

Creating


Course

Learning

Outcomes

Program Learning Outcomes (PLOs) Program Specific Outcomes

(PSOs)

PL

O1

PL

O2

PL

O3

PL

O4

PL

O5

PL

O6

PL

O7

PL

O8

PL

O9

PL

O1

0

PL

O1

1

PL

O1

2

PS

O1

PS

O2

PS

O3

PS

O4

CLO1 L H L H H M M H M L L

CLO2 H M L L H L H M H H H M

CLO3 L H M M H L L H H H L L M



Seminar-II

MBT 2504

L T P C

0 0 1 1





of biotechnology.






be able to

CLO Description

Bloom’s

Taxonomy

Level


topic selected

4

Analyzing



2, 5

Understanding,

Evaluating



6

Creating



2, 6

Understanding,

Creating


Course

Learning

Outcomes

Program Learning

Outcomes (PLOs)

Program Specific

Outcomes (PSOs) P

LO

1

PL

O2

PL

O3

PL

O4

PL

O5

PL

O6

PL

O7

PL

O8

PL

O9

PL

O1

0

PL

O1

1

PL

O1

2

PS

O1

PS

O2

PS

O3

PS

O4

CLO1 H L H H M L L

CLO2 H M L H H H M

CLO3 M H L H L L M



S.

No.

Subject


THEORY

1. MBT3005 Industrial and Pharmaceutical

Biotechnology 4 0 - 40 60 100 4

2. MBT3006 Enzyme Engineering and

Technology 4 0 - 40 60 100 4

3. MBT3007

Ethical Issues in

Biotechnology and

Engineering

3 0 - 40 60 100 3

4. MBT3008 Technical Writing and

Literature Survey 2 0 - 20 30 100 2

5. --- Department Elective – III 3 0 - 40 60 100 3


6. MBT3507 Biochemical Preparations Lab 0 0 4 80 20 100 2

7. MBT3508 Project Work Phase-I 0 0 4 80 20 100 2

8. MBT3503 Seminar-III 0 0 1 100 0 100 1

Total 16 0 9 440 310 750 21

Every student or a group of 3-4 students will be given a mentor, as decided by a Committee constituted by the Director, IBST, based

on the inputs provided by the students, at the end of the II or beginning of III semester. Students will be asked to give their choice if

they are interested in doing the project at the university or outside the university at a recognized research institution. The university

will place the students who choose to do their projects outside the university at research institutes or universities of repute where high-

impact research is conducted. The students who choose to do their projects at the university will be asked to decide a supervisor from

the department. The supervisor will identify a suitable research topic and assign the same to the student. The supervisor will help the

student in literature survey, in making a research plan, and doing preliminary or validation experiments, etc as per the requirements of

the project.

M. Tech. Biotechnology II Year: III Semester

University Mandatory Non-Credit Course

1. XHUX601 Human Values and Ethics 2 - - 100 - 100 0

Industrial and Pharmaceutical Biotechnology

MBT 3005

L T P C

4 0 0 4


• The syllabus will help the students to be familiar with various biotech industries

dealing with processing and production of products, obtained from natural and

synthetic drugs.

• Students will understand the various techniques used in modern pharmaceutical

biotechnology.

• This course tries to create a synergy between industrial pharma and industrial

biotechnology.

• Syllabus will help in focusing on the pharmaceutical, medical and therapeutic

properties of numerous biopharmaceutical products.

UNIT I: [8 Hours]

Introduction to Pharmaceutical industries

Industrial aspects: Pharmaceutical biotechnology information- biopharmaceutical

drugs & biotechnology products made by biotech companies,Stability studies of

biotechnology derived products, Effects of various environmental /processing on

stability of the formulation and techniques for stabilization of product against the same

regulatory requirement related to stability testing.

UNIT II: [8 Hours]

Broad spectrum of Pharmaceutical products

Herbal and naturally derived Products: ‐ Formulation development aspects, Delivery

aspects for herbal and naturally derived medicinal products (Herbal extracts, crud

extracts, incorporation of product performance enhancers, etc.). Synthetic and

Semisynthetic medicines, Formulation development aspects, Delivery aspects for

Synthetic and Semisynthetic medicines, Regulatory considerations with consideration

of global regulatory guidelines.

UNIT III: [8 Hours]

Quality check and control in Pharma industries

General Considerations, Spectroscopy and Assay development, dissociation,

partitioning and Solubility of Pharmaceutical Solids, pKa, salts, solvents, Ko/w, drug

design, phase solubility analysis, solubilization, release, dissolution and permeation,

chiral drug substances, characterization scheme.

UNIT IV: [8 Hours]

Pharmaceutical drugs with focus on enhancing shelf life

Dosage form consideration in pre-formulation, solid dosage form, solution

formulations, emulsion, suspension, freeze dried products, topical, pulmonary,

evaluations and its regulatory considerations, stability tastings, order of reaction,

antioxidants, chelating agents, impurity, GMP related to bulk drugs and APIs.

UNIT V: [8 Hours]

Pharmaceutical drugs for treating the diseases

Industrial application of biotech products: industrial enzymes (examples),

immobilization of enzymes, their applications in industry, Immobilized Enzyme

engineering, Kinetics of immobilized enzymes, novel methods for enzyme and

vaccine production.

TEXT BOOK:

1. Pharmaceutical Biotechnology: Fundamentals and Applications, edited by Daan

J.A. Crommelin, Robert D. Sindelar, Bernd Meibohm, fundamental and

applications, 4th edition, 2013 Springer.

2. Biotechnology: Expanding Horizons B.D. Singh, Kalayani Publishers,

Biotechnology 2010.

REFERENCE BOOKS:

1. Comprehensive Biotechnology (Vol. 1-4): M.Y. Young (Eds.), Pergamon Press,

Oxford, 2007.

2. Introduction to the Pharmaceutical Sciences, By Nita K. Pandit, Lippincott

Williams & Wilkins, 2007.

https://www.google.co.in/search?tbo=p&tbm=bks&q=inauthor:%22B.+D.+Singh%22

https://www.google.co.in/search?tbo=p&tbm=bks&q=subject:%22Biotechnology%22&source=gbs_ge_summary_r&cad=0

Course Learning Outcomes (CLO): On completion of this course, the students will be able

to:


Taxonomy Level

CLO1 Analyze scientific principles underlining modern

biotechnology subject aimed specifically at

pharmacy and medicines.

4

Analyzing

CLO2 Design research strategy with step -by -step

instructions to address a research problem.

6

Creating

CLO3 Provide examples of current applications of

biotechnology and advances in the different areas

like medical, microbial, environmental,

bioremediation, agricultural, plant, animal, and

virology.

5,

Evaluating

CLO4 Modify and improve their learning and

understanding of the subject

6

Creating


Course

Learning

Outcomes

Program Learning

Outcomes (PLOs)

Program Specific

Outcomes(PSOs) P

LO

1

PL

O2

PL

O3

PL

O4

PL

O5

PL

O6

PL

O7

PL

O8

PL

O9

PL

O1

0

PL

O1

1

PL

O1

2

PS

O1

PS

O2

PS

O3

PS

O4

CLO1 H L M

H M M M

CLO2 M L L M H

H M M M

CLO3 L M M

H M M M

CLO4 M M M M M M

H M


Enzyme Engineering and Technology

MBT 3006

L T P C

4 0 0 4


• This course is designed to equip the students with Advance knowledge of

Enzyme Kinetics, Optimization and modeling of Enzyme Systems.

• Students will be able to design enzymatic process, after the completion of the

course.

• Enzyme engineering course focuses from isolation to modification of enzyme

by the applications of biotechnology.

• Syllabus will make student industry ready.

UNIT I: [8 Hours]

Properties of Enzymes

Introduction, nomenclature and scope of Enzymes. Mechanism of enzyme action,

concept of ES complex, Active sites. Specificity of enzymes, Enzyme UNITs and

turn over number.

UNIT II: [8 Hours]

Enzyme Kinetics

Order of reactions, kinetics of enzyme reactions- single, bi substrate reactions.

Michaelis- Menten Equation. Estimation of Km and Vmax and their physiological

significance. Allosteric enzymes, their role in controlling metabolic pathways.

UNIT III: [8 Hours]

Enzyme Immobilization

Physical and chemical techniques for enzyme immobilization- adsorption, matrix-

entrapment, encapsulation, cross linking and covalent bonding etc. Advantages and

disadvantages of enzymes immobilization. Temperature dependence of enzymatic

rate constant, thermal deactivation. pH dependence of enzymes- ionization of acids

and bases.

UNIT IV: [8 Hours]

Isolation, Purification and Characterization of Enzymes:

Isolation, purification and characterization of enzymes. From natural sources.

Preparation of purification chart once the purification is done. Different methods of

enzyme characterization- SDS-PAGE, 2-D etc. Case studies and discussion on the

research papers based on enzyme isolation.

UNIT V: [8 Hours]

Industrial Applications of Enzymes

White Biotechnology: Examples of enzyme applications in industrial process,

production of drugs and fine chemicals. Enzyme based Biosensors. Enzymes in

organic catalysis. Enzyme Engineering, site directed mutagenesis and other methods

of enzyme production. In vitro methods of enzyme modifications by protein

expression.

TEXT BOOKS:

1. Voet and Voet (1990) Biocehmistry John Wiley & Sons, New YOrk

2. Palmer T and Bonner TL (2007) Enzymes: Biochemistry, Biotechnology and

Clinical Biochemistry,

REFERENCE BOOKS:

1. Jeremy Mark Berg, John l., Tymoczko and Stryer L., (2002) Biocehmistry WH

Freeman New York

2. Harrison R. G., Todd P., Rudge S. R. (2003) Bioseparation Science and

Engineering, Oxford Press, Oxford.



Taxonomy Level

CLO1 Analyze the data obtained for enzymes, appreciate

the utility of enzymes in various industries.

4,

Analyzing

CLO2 Design new enzymes for future.

6,

Creating

CLO3 Optimize the conditions for improving the enzyme

production and making them tolerant to harsh

conditions.

6,

Creating

CLO4 Interpret the laws of enzyme kinetics. 3,

Understand


Course

Learning

Outcomes

Program Learning

Outcomes (PLOs)

Program Specific

Outcomes(PSOs)

PL

O1

PL

O2

PL

O3

PL

O4

PL

O5

PL

O6

PL

O7

PL

O8

PL

O9

PL

O1

0

PL

O1

1

PL

O1

2

PS

O1

PS

O2

PS

O3

PS

O4

CLO1 H L M

H M M M

CLO2 M L L M H

H M M M

CLO3 M M

H M M M

CLO4 M M M M M M

H M


Ethical Issues in Biotechnology and Engineering

MBT 3007

L T P C

3 0 0 3


• The course is designed to outline the ethical issues underlying biotechnology

research and innovation. The specific objectives of the course are:

• To caution the nature of hazards related to biotechnology and the importance

of bioethics in research.

• To inspire individuals to choose their own personal, social, moral and spiritual

values and be aware of practical methods for developing tools and techniques

in Biotechnology.

• It will help student develop society above individual concept.

UNIT I: [7 Hours]

Introduction to bioethics

Definition of bioethics; need of bioethics; bioethics and its relation with other

branches; application of bioethics; socio-economic impacts of biotechnology:

agriculture and food sector, institutional issues, social issues, cultural issues,

business and consumer issues, biodiversity issues.

UNIT II: [7 Hours]

Bioethical issues related to human genome project and disease diagnosis

Introduction to human genome project (HGP); future of medicine in HGP; ethical,

legal and social implications (ELSI) of HGP; molecular detection of pre-

symptomatic genetic diseases and its implication in healthcare; prenatal diagnosis,

genetic manipulation and their ethical issues.

UNIT III: [7 Hours]

Bioethical issues related to genetically modified organisms

Introduction to genetically modification organisms (GMOs); present scenario of

GMOs; future of GMOs; developing agreed national and international instruments

of governance; GMOs and scientific responsibility; food crops; GM products:

benefits and controversies; present problems and future governance of GM

Technology.

UNIT IV: [7 Hours]

Bioethical issues related to stem cell research and animal testing

Introduction to stem cells; ethical issues of stem cell research and use; animal

research and testing; animal models; genetically modified animals and their use in

research and testing; ethical issues in the use of animals in research and testing;

ethical issues in research involving human trial.

UNIT V: [7 Hours]

Bioethical issues related to organ transplantation and cloning

Introduction to organ transplantation; sources of organs; transplantation and its

ethical and social issues; legislation and transplantation; cloning and its application;

human cloning; arguments against and in defense of human cloning; ethics of adult

human DNA cloning

TEXTBOOKS:

1. Sateesh MK. 2010. Bioethics and Biosafety. IK International Pvt Ltd.

2. Sree Krishna V. 2007. Bioethics and Biosafety in Biotechnology. New age

international

REFERENCE BOOKS:

Morris J. 2006. The Ethics of Biotechnology. Infobase Publishing Sherlock R. 2002. Ethical Issues in Biotechnology. 2002. Rowman & Littlefield

Course Learning Outcomes (CLO):On completion of this course, the students will be

able to:


Taxonomy Level

CLO1 Apply ethical principles and commit to professional

ethics and responsibilities and norms of the

engineering and technology practice.

3,

Applying

CLO2 Understanding of the professional and ethical

responsibilities of the Biotechnologist.

2

Understanding

CLO3 Ensure the quality of products and their impacts and

influence on the environment should be sustainable.

5,

Evaluating

CLO4 Solve issues related to ethics. 6,

Creating


Course

Learning

Outcomes

Program Learning

Outcomes (PLOs)

Program Specific

Outcomes(PSOs)

PL

O1

PL

O2

PL

O3

PL

O4

PL

O5

PL

O6

PL

O7

PL

O8

PL

O9

PL

O1

0

PL

O1

1

PL

O1

2

PS

O1

PS

O2

PS

O3

PS

O4

CLO1 H L M

H M M M

CLO2 M L L M H

H M M M

CLO3 L M M

H M M M

CLO4 M M M M L

H M


Technical Writing and Literature Survey

MBT 3008

L T P C

2 0 0 2


• This course is designed to provide the fundamental knowledge of writing a

document.

• Preparation of manuscripts for publications in the form of Research Paper,

Review Articles.

• While doing so students are expected to perform the Review of Literature,

Compilation and searching of the primary and secondary data.

UNIT I: [5 Hours]

Technical Writing

Introduction of Technical writing, types of documents, Importance of Technical

writing. Role of Teacher and researcher in Technical writing. Documentation

process, evolution of technical writing. Collection of literature and compilation of a

document.

UNIT II: [5 Hours]

Technical Writing Software

Processing of the Text collected with the help of various softwares like- MS-word,

Text processing. Properties of a good text document. Checking the documents for

proper usage of language and spellings.

UNIT III: [8 Hours]

Literature Survey

Introduction of Literature Review, steps of Reviewing, Collection and storage of

Review searched. Citation of searched Literature. Keeping your reference ready for

publication with the help of Googlescholar or any other tools.

TEXT BOOK:

Basu B. N. (2007) Technical Writing PHI Publication PVt. Ltd,

Course Learning Outcomes (CLO):On completion of this course, the students will be able to:


Taxonomy Level

CLO1 Collect and build the scientific literature on a

particular subject.

6,

Creating

CLO2 Write down the scientific project and reports for their

Project work and relate with it.

2

Understanding

CLO3 Present and show their research in the proper manner

at various conferences.

2

Understanding

,

CLO4 Develop better knowledge about theory as well as

practical aspect.

3,6

Understand

Creating


Course

Learning

Outcomes

Program Learning

Outcomes (PLOs)

Program Specific

Outcomes(PSOs) P

LO

1

PL

O2

PL

O3

PL

O4

PL

O5

PL

O6

PL

O7

PL

O8

PL

O9

PL

O1

0

PL

O1

1

PL

O1

2

PS

O1

PS

O2

PS

O3

PS

O4

CLO1 H L M

H M M M

CLO2 M L L M H

H M M M

CLO3 M M

H M M M

CLO4 M M M M M M

H M


Bioreactor Design and Analysis

MBT 3105

L T P C

3 0 0 3


• The course imparts advanced knowledge on bioreactor design for efficient

utilization of the principles in bioprocess technology.

• Course includes the construction materials, the flow of liquid and their types.

• Course also includes the types of products formed, their mathematical model

based optimization.

UNIT I: [7 Hours]

Stirred tank bioreactors

Introduction to ideal and non-ideal bioreactors; Design, construction and operation

of a typical aseptic, aerobic continuous stirred tank reactor; Chemostat with recycle,

Multistage chemostat systems, Fed batch operation, Perfusion systems.

UNIT II: [7 Hours]

Air-driven bioreactors and other bioreactor designs

Design and operation of: Airlift bioreactor, Bubble column bioreactor, Fluidized bed

bioreactor, Trickle bed bioreactor, Packed bed bioreactor, Photo bioreactors, Plug

flow reactors; Alternate bioreactor configurations.

UNIT III: [7 Hours]

Animal and plant cell bioreactors

Animal cell bioreactor: Environmental requirements, Bioreactor considerations for

animal cell culture, Bioreactors for large scale production using animal cells. Plant

cell bioreactor: Bioreactors for suspension cultures, Reactors using cell

immobilization, Bioreactors for organized plant tissue.

UNIT IV: [7 Hours]

Solid state fermentation bioreactors and immobilized cell systems

Comparison between solid-state fermentations and submerged fermentation, Types:

Tray bioreactor, Packed-bed bioreactors, Rotating-drum and stirred-drum

bioreactors, Mixed and forcefully-aerated bioreactors. Immobilized cell systems:

Immobilized biocatalyst, Formulation and characterization of immobilized cell

biocatalyst, Applications.

UNIT V: [7 Hours]

Instrumentation control of bioreactors

Monitoring and control of bioreactors: Online and offline analysis, Use of

biosensors, Measurement analysis, Fault analysis, Process modelling, State

estimation, Feedback control, Indirect metabolic control, Programmed control,

Artificial intelligence control, fuzzy logic control.

TEXT BOOKS:

1. Bailey J. E. and Ollis D. F. 1986. Biochemical Engineering Fundamentals, 2nd

ed. McGraw Hill.


REFERENCE BOOKS:

1. Shuler M. L. and Kargi F. 2002. Bioprocess Engineering: Basic Concepts. 2nd

ed. Prentice Hall Inc.





Taxonomy Level

CLO1 Design and analyze batch, continuous flow, and fed

batch reactors with specific instrumentation required

for the efficient monitoring and control of simple

bioreactor, ancillary equipment required for the

aseptic feeding, sampling and processing of

bioreactor fluids.

3,4

Creating,

Analyzing

CLO2 Learn the design of solid state fermentation

bioreactors and instrumentation control of

bioreactors and design biological reactors with cell

recycle streams.

6

Creating

CLO3 Learn about thefunction of Air-driven bioreactors

and bioreactors for plant and animal cell cultivation

3

Analyzing

CLO4 Improve their learning skills. 6

Creating


Course

Learning

Outcomes

Program Learning

Outcomes (PLOs)

Program Specific

Outcomes(PSOs) P

LO

1

PL

O2

PL

O3

PL

O4

PL

O5

PL

O6

PL

O7

PL

O8

PL

O9

PL

O1

0

PL

O1

1

PL

O1

2

PS

O1

PS

O2

PS

O3

PS

O4

CLO1 H L M

H M M M

CLO2 M L L M H

H M M M

CLO3 M M

H M M M

CLO4 M M M M M M

H M


Biochemical and Biophysical Techniques

MBT3106

L T P C

3 0 0 3 Course Learning Objectives:

• The course imparts advanced knowledge on Biopolymers and their properties.

• Course includes the techniques used to visualize microorganisms.

• Course also includes the types of electrophoresis methods used for protein, nucleic acid.

• The course will also introduce the separation and sequencing techniques.

UNIT I: [6 Hours]

Colloids of Biopolymers and Their Properties

Colloidal solutions of biopolymers and their electrochemical properties. Hydrodynamic properties:

Viscosity, diffusion etc of biopolymers; Molecular weight determination, osmotic pressure, reverse

osmosis, and Donnan effect. Structure of Biomembranes and their electrochemical properties,

membrane potential, action potential and propagation of impulses.

UNIT II: [6 Hours]

Microscopic Techniques

Introduction to principles and working of light & Electron Microscope, Scanning Tunneling

Microcopy, SEM, TEM, Atomic force Microscopy, Sample preparation for Electron Microscopy.

UNIT III: [6 Hours]

Electrophoresis & Advanced Immuno Techniques

Different methods of electrophoresis for protein, nucleic acids, small molecular weight

compounds. Peptide mapping and combination of electro focusing and SDS-PAGE, Comet assay,

Karyotyping, FISH, Rocket Immunoelectrophoresis, ELISA, RIA, western blot.

UNIT IV: [6 Hours]

Spectrophotometry and Radio Activity

Introduction to principles and applications of spectroscopic techniques (UV, Vis, IR, Fluorescence,

ORD,CD, PAS, NMR, ESR & Mass spectrometry) Use of radioactive and stable isotopes and their

detection in biological systems.

UNIT V: [6 Hours]

Separation and Sequencing Techniques

Automatic analyzer for amino acids, protein sequencer, peptide synthesizer & nucleic acid

synthesizer. Cell sorters and their applications. Theory of lyophilization and its applications to

biological systems.

TEXT BOOKS

1. Pranab Kumar Banerjee, 2008, Introduction to Biophysics S Chand and company,

2. G. R Chatwal and S .K Anand, 2008, Instrumental methods of chemical analysis Himalaya

publishing house.

REFERENCE BOOKS

1. S. Harisha, 2008, Biotechnology Procedures and Experiments handbook by Infinity Science

Press LIC.



Taxonomy Level

CLO1 Classify different microscopic techniques used to

visualize micro objects. 2

Understanding

CLO2 Identify a core knowledge based on the theory of

modern Biochemistry and Biophysics. 3

Applying

CLO3 Distinguish among different methods of

electrophoresis for protein, nucleic acids, small

molecular weight compounds.

4

Analyzing

CLO4 Discuss the principle and working of the separation and

sequencing techniques. 6

Creating


Course

Learning

Outcomes

Program Learning

Outcomes (PLOs)

Program Specific

Outcomes(PSOs)

PL

O1

PL

O2

PL

O3

PL

O4

PL

O5

PL

O6

PL

O7

PL

O8

PL

O9

PL

O1

0

PL

O1

1

PL

O1

2

PS

O1

PS

O2

PS

O3

PS

O4

CLO1 H M L L M

L M L M

CLO2 M L L M H L

H L L L

CLO3 M M M

H M L M

CLO4 M L

M L L M


Biochemical Preparation Lab

MBT 3507

L T P C

0 0 4 2


• The objective of this course is to impart knowledge to students on the use of

various analytical instruments which are commonly used for separation,

identification and quantification of biochemicals.

• Course will help students to understand basic chromatographic technique.

• Students will be able to perform lab experiments.

• Students will be more concern about the error and their side effects during

chemical preparation.

List of experiments

1. Preparation and purification of acetylsalicylic acid (Asprin)

2. Analyses of aspirin product by thin layer chromatography (TLC)

3. Melting point analysis of Asprin crystals.

4. Detection of adulteration in given milk sample.

5. Determination of chemical parameters of water samples.

6. Proximity analysis of fresh fruit samples.

7. Chemical analysis of fruit juice samples.

TEXT BOOKS:

1. Pranab Kumar Banerjee, 2008, Introduction to Biophysics S Chand and

company,

2. G. R Chatwal and S .K Anand, 2008, Instrumental methods of chemical

analysis Himalaya publishing house.

REFERENCE BOOKS:

S. Harisha, 2008, Biotechnology Procedures and Experiments handbook by

Infinity Science Press LIC.



Taxonomy Level

CLO1 Differentiate experiment with/ between the uses of

various instruments for sample analysis.

3,6

Applying,

Analyzing

CLO2 Evaluate the effect of the errors in the calculations

5

Evaluating

CLO3 Inspect, understand and interpret the

results/Calculation for the experiment in the form of

observation.

3,2, 5

Understanding,

Applying,

Analyzing

CLO4 Interpret the results. 3,

Understand


Course

Learning

Outcomes

Program Learning

Outcomes (PLOs)

Program Specific

Outcomes(PSOs) P

LO

1

PL

O2

PL

O3

PL

O4

PL

O5

PL

O6

PL

O7

PL

O8

PL

O9

PL

O1

0

PL

O1

1

PL

O1

2

PS

O1

PS

O2

PS

O3

PS

O4

CLO1 H L M

H M M M

CLO2 M L L M H

H M M M

CLO3 M M

H M M M

CLO4 M M M M M M

H M


Project Work Phase-I

MBT 3508

L T P C

0 0 4 2


• The objective of this course is to develop testable hypothesis and research

design for deriving apt research conclusions.

• To design and conduct the quantitative as well as qualitative research either in

laboratory or in fields.

• To utilize the research data for the formulation and evaluation of new research

questions.



Taxonomy Level

CLO1 Discuss wholesome research leading to findings and apply for

the production of new and improved products. 2,3

Understanding,

Applying

CLO2 Develop skills to do proper research in various fields of life-

sciences. 3

Applying

CLO3 Analyze and Assess work opportunities emerging in

Biotechnology. 4,5

Analyzing,

Evaluating

CLO4 Adapt the skills for their core and IT related companies

interviews. 6

Creating


Course

Learning

Outcomes

Program Learning

Outcomes (PLOs)

Program Specific

Outcomes(PSOs) P

LO

1

PL

O2

PL

O3

PL

O4

PL

O5

PL

O6

PL

O7

PL

O8

PL

O9

PL

O1

0

PL

O1

1

PL

O1

2

PS

O1

PS

O2

PS

O3

PS

O4

CLO1 L L H L

H H H M

CLO2 L M M M H M

H H M H

CLO3 L L L

H H M M

CLO4 L L H

H M M H


ENTREPRENEURSHIP FOR BIOTECHNOLOGY

MMG3005

(40 Hours)

Course Objective:

• To train students in the art of converting an idea into a business.

• To give an overview of various processes which are required to start an innovative business-

for product and/or services.

• To inform them about the governmental support for initiating new ventures.

UNIT-I (10 Hours)

Entrepreneurship and New Venture Opportunities, Entrepreneurship & Innovation- Creativity as a

prerequisite to Innovation, Entrepreneurship vs. Entrepreneurship, Characteristics of

Entrepreneurs, Entrepreneur vs. Manager, Functions of an Entrepreneur in the economic

development of a country. Model for new ventures- feasibility planning- The four stage Growth

Model. Reasons for Entrepreneurial failure.

UNIT -II (08 Hours)

The product concept and Commercial opportunities (Opportunity Identification)- Importance of

manufacturing, products and technology, the product development process. Product Protection:

Patents, Trademarks and Copyrights. Services and Commercial opportunities- Infrastructure of

services, types of service ventures, success factors for service ventures.

UNIT-III (12 Hours)

Marketing Research for new ventures- market research in pre-startup phase, sources of market

intelligence, Competitive analysis and implications. Marketing Functions- product concepts,

distribution, promotion, pricing. Marketing strategies. International Markets and new venture

opportunities.

UNIT-IV (10 Hours)

Organizing – Team Building, Legal forms of business. Financing the new venture, exploring

various sources of funding, Business acquisition and franchising, Managing, growing and ending

the new venture.

Case Study: Biocon- India’s largest biopharmaceutical company.

TEXT BOOKS:

T1. Manimala, Peters, Shepherd &Hisrich- Entrepreneurship, McGraw Hill, 9e

T2. David H. Holt- Entrepreneurship- New Venture Creation- PHI

L T P C

3 0 0 3

REFERENCE BOOKS:

R1. Hisrich, Peters & Shepherd- Entrepreneurship- 6th Edition

R2. Barringer, Entrepreneurship: Successfully launching new ventures, Pearson 3rd Edition

R3. Charantimath, Entrepreneurship: Development and Small Business Enterprise, Pearson

1st Edition.

R4. Rajeev Roy, Entrepreneurship, Oxford.

R5. Pareek Udai, & Venkateshwara Rao- Developing Entrepreneurship- A Handbook on

Learning Systems, Learning Systems.

R6. Rai- Entrepreneurship Development (Vikas Publication)

R7. Clifton & Bharadwaj, Entrepreneurial Strength Finder,1st,Gallup Press,2014

R8. Siddhartha Sharma, The Millionaire Entrepreneur- Like a Boss,1st,Learners of Life

Publication,2013

R9. Puneet Srivastava, Accidental Entrepreneur,1st,Rupa& Co.,2012


able:


Taxonomy Level

CLO1 Describe the concept of Entrepreneurship and its importance

to the economy for enhancing productivity and creating jobs.

1,2

Remembering,

Understanding

CLO2 Differentiate between entrepreneurship and business and the

skills required to be successful as an entrepreneur

2, 4

Understanding,

Analyzing

CLO3 Apply the concepts to identify entrepreneurial opportunity

and create a niche for the enterprise

2, 3

Understanding,

Applying

CLO4 Explain the relevance of intellectual property for an

entrepreneur

2, 3, 4

Understanding,

Applying,

Analyzing

CLO5 Formulate the marketing strategy, identify international

opportunities and configure the team for organizational

succes

3,5

Applying

Evaluating

CLO6 Evaluate financial resources, decide the appropriate legal

format for conduction business and develop competency for

managing the venture through the different phases of its life

cycle

2, 4, 6

Understanding,

Analyzing

Evaluating


Course

Learning

Outcomes

Program Learning Outcomes

(PLOs)

Program Specific

Outcomes(PSOs)

PL

O1

PL

O2

PL

O3

PL

O4

PL

O5

PL

O6

PL

O7

PL

O8

PL

O9

PL

O10

PL

O1

1

PL

O12

PS

O1

PS

O2

PS

O3

PS

04

CLO1 L M M M M L L M M M

CLO2 M M M M M M M L L L

CLO3 L L M L M M M M M M M M

CLO4 L L M M M M M M H M L L

CLO5 L M M M M H M M M M L

CLO6 L M M M M M H M M M L


SEMINAR-III

MBT 3503

L T P C

0 0 1 1





of biotechnology.






be able to

CLO Description

Bloom’s

Taxonomy

Level


topic selected

4

Analyzing



2, 5

Understanding,

Evaluating



6

Creating



2, 6

Understanding,

Creating


Course

Learning

Outcomes

Program Learning

Outcomes (PLOs)

Program Specific

Outcomes (PSOs)

PL

O1

PL

O2

PL

O3

PL

O4

PL

O5

PL

O6

PL

O7

PL

O8

PL

O9

PL

O1

0

PL

O1

1

PL

O1

2

PS

O1

PS

O2

PS

O3

PS

O4

CLO1 H L H H M L L

CLO2 H M L H H H M

CLO3 M H L H L L M



Human Values and Ethics

XHUX 601

L T P C

2 0 0 0


• To make students recall and apply the basic human values.

• To recommend them a training in human relationship for appreciating family and society.

• To organize and prioritize them in a manner in which they can be adaptable to the nature.

• To develop the skills to identify the characteristics of people and the Eco-friendly

production system.

UNIT-I (06 Hours)

Requirement of Value Education, Fundamental Guidelines, Content, and Methodology for

Value Education

Appreciating the need, Fundamental Guidelines, Content and Methodology for Value Education,

Self-Investigation: Methodology and Content. Fundamental Human Aspirations: Uninterrupted

Happiness and Prosperity. A Critical Evaluation of the present situation: Understanding Happiness

and Prosperity rightly, Harmonious Living at all levels.

UNIT-II (04 Hours)

Understanding Harmony in the Individual - Harmony within Self An

individual as Co-existence of Sentient Self (‘I’) and Physical Body. Learning by distinguishing the needs of Self (‘I’) and ‘Body’ - Happiness and Prosperity

Problems related to identity crisis, depression, lack of motivation, traumatic childhood.

Harmony of Self (‘I’) with the physical Body: Self-control and Health; Understanding Physical

Needs. Understanding suicide and psychosomatic problems. UNIT– III (06 Hours)

Human Relationship: Appreciating Harmony in Family and Society

Fundamentals for Harmony in Family as basic unit of human interaction Understanding values in

human to human relationship. Family problems, domestic violence, relationship issues among

youth. Understanding society as an extension of Family;Social crimes; causes, factors leading to

such. UNIT- IV (06 Hours)

Existence as Co-existence; Understanding Harmony in the Nature and Existence

Harmony in Nature and its critical appraisal in the present scenario.

UNIT– V (06 Hours)

Positive Psychology & Professional Ethics

Competence in Professional Ethics:

a) Capacity to utilize the professional competence for expanding Universal Human Order

b) Skill to identify the scope and characteristics of people-friendly and eco-friendly production

systems

c) Ability to identify and develop appropriate technologies and management Patterns for above

production systems

Text Book:

T1. Gaur, R.R.,Sangal, R., and Bagaria, G.P. (2009) “A Foundation Course in Human Values and

Professional Ethics” Excel Books Private Limited. New Delhi.

Reference Books:

R1.Illich, I.(1974) “Energy & Equity” The Trinity Press, Worcester and Harper Collins,USA.

R2.Schumacher, E.F. (1973) “Small is Beautiful: A Study of Economics as if People Mattered”

Blond & Briggs, Britain.

R3.Sussan, G.(1976) “How the Other Half Dies”, Penguin Press, Reprinted 1991.

R4.Meadows, D.H.,Meadows, D.L. Randers, J., Bchrens, W.W.III (1972) “Limits lo Growth

Club of Rome’s report”, Universe Books.

R5.Nagraj, A. (1998) “Jeevan VidyaekParichay” Divya Path Sansthan, Amarkantak.

R6.SeebauerE.G. and Berry, R.L. (2000) “Fundamentals of Ethics for Scientists &Engineers”

Oxford University Press.


able to:


Taxonomy Level

CLO1 Requirement of Value Education, Fundamental Guidelines,

Content, and Methodology for Value Education- Explain the need, Fundamental Guidelines, Content and

Methodology for Value Education, Self-Inspect: Methodology and

Content. Fundamental Human Aspirations: Uninterrupted

Happiness and Prosperity. A Critical Evaluation of the present

situation: Understanding Happiness and Prosperity rightly,

Harmonious Living at all levels.

2, 4, 6 Understanding

Analyzing, Evaluating

CLO2 Outlining Harmony in the Individual -Harmony within Self -An

individual as Co-existence of Sentient Self (‘I’) and Physical Body. Learning by distinguishing the needs of Self (‘I’) and ‘Body’ -

Happiness and Prosperity Problems related to identity crisis, depression, lack of motivation,

traumatic childhood. Harmony of Self (‘I’) with the physical Body: Self-control and

Health; Interpret Physical Needs. Analyzing suicide and

psychosomatic problems.

2,5, 4, Understanding,

Evaluating

Analyzing

CLO3 Human Relationship: Demonstrating Harmony in Family and

Society

Fundamentals for Harmony in Family as basic unit of human

interaction Identifying values in human to human relationship.

Family problems, domestic violence, relationship issues among

youth. Understanding society as an extension of Family; Social

crimes; causes and determining factors leading to such problems.

2,3, 5,

Understanding,

Apply

Evaluating

CLO4

Existence as Co-existence; Illustrate Harmony in the Nature and

Existence. Harmony in Nature and its critical appraisal in the

present scenario.

2,5,

Understanding Evaluating,

CLO5 Positive Psychology & Professional Ethics

Competence in Professional Ethics: a) Developing skill to utilize the professional competence for

expanding Universal Human Order

b) Skill to identify the scope and characteristics of people-friendly

and Eco-friendly production systems.

c) Ability to identify and prioritize appropriate technologies and

management Patterns for above production systems

6,3,5

Creating,

Applying,

Evaluating


Course

Learning

Outcomes

CLOs

Programme Learning Outcomes (PLOs) Programme Specific Outcomes

(PSOs)

PL

O1

PL

O2

PL

O3

PL

O4

PL

O5

PL

O6

PL

O7

PL

O8

PL

O9

PL

O1

0

PL

O1

1

PL

O1

2

PS

O1

PS

O2

PS

O3

PS

O4

PS

O5

PS

O6

CLO1 H H M M M H H L H H M L H M L L L L

CLO2 H M M M H H H L H H L H M M H M M L

CLO3 M H H M M H H M M H H M M H H H H H

CLO4 M H H H M H H M M M M M H H H H H H


Project Work Phase-II

S.

No.

Subject



1. MBT4507 Project work Phase-II - - 32 50 50 100 16

Total 0 0 32 50 50 100 16

During the IV semester students will do their project work. The internal evaluation of students doing their projects

at the university will be conducted by attendance monitoring and research progress. The internal evaluation of

students doing their projects outside the university will be based upon the fortnightly/monthly reports sent via email

by external supervisors. The external supervisors will be requested to send a very brief report of the student to the

university mentor. The students who get jobs during the IV semester will be required to submit the documents

supplied by their employer (as per the company policy) as part of the internal evaluation. After the completion of

project work viva-voce will be conducted along with practical examinations by an external examiner appointed by

the Director, IBST.


II Year: IV Semester

MBT 4507

L T P C

0 0 32 16


• The students will learn to perform experiments as an individual.

• The students will perform application based understanding about the

experiments and their analysis by reading the research papers.

• The students can finalize the reports and present them.



Taxonomy Level

CLO1 Discuss wholesome research leading to findings and

apply for the production of new and improved

products.

2,3

Understanding,

Applying

CLO2 Develop skills of paper writing and presentation and

interpret the research data. 2,3

Understanding,

Applying

CLO3 Analyze and Assess work opportunities emerging in

Biotechnology. 4,5

Analyzing,

Evaluating

CLO4 Adapt the skills for their core and IT related

companies interviews and they will be able to design

the research projects.

6

Creating

STUDY & EVALUATION SCHEME (Effective from the session 2017-2018)


Course

Learning

Outcomes

Program Learning

Outcomes (PLOs)

Program Specific

Outcomes(PSOs)

PL

O1

PL

O2

PL

O3

PL

O4

PL

O5

PL

O6

PL

O7

PL

O8

PL

O9

PL

O1

0

PL

O1

1

PL

O1

2

PS

O1

PS

O2

PS

O3

PS

O4

CLO1 L L H L

H H H M

CLO2 L M M M H M

H H M H

CLO3 L L L

H H M M

CLO4 H L L H

H M M H


STUDY & EVALUATION SCHEME (Effective from the session 2017-2018)

List of Department Electives

S. No. Subject Code Subject

Department Elective-I [Semester-II]

1. MBT2105 Nanobiotechnology and Nanodevices

2. MBT2106 Microbial Engineering

Department Elective-II [Semester-II]

1. MBT2202 Biocatalysis and Enzyme Reaction

2. MBT2203 Animal Cell Science and Technology

3. MBT2204 Cell and Tissue Engineering

Department Elective-III [Semester-III]

1. MBT3105 Bioreactor Design and Analysis

2. MBT3106 Biochemical and Biophysical Techniques


M. Tech Biotechnology Syllabus w. e. f.

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Transcript of M. Tech Biotechnology Syllabus w. e. f.