Live Sense 2010
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Transcript of Live Sense 2010
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7/26/2019 Live Sense 2010
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Autonomous cell-based biosensor microsystem
A week of autonomy (?)
Communication unit (to GSM device)
Versatile for various cell models and secondary sensors
Cell-based biosensors provide a biologically relevant
response to toxic compounds and mixtures
CellsCells optical
sensors
nutrient
supply
signal
processing
unit
comm.
unitelectrical
sensors
sampling
data com
microsystem platformbioreactor
power supply
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78" 9 +"&.' /- 4/5"6"-'" :2,;"1+
EPFL-LMIS Microsystems Laboratory Ph. Renaud
Microfluidics, cell chips, bio-impedance
UNIL-DMF Department of Fundamental Biology J. van der Meer
Cell biology, gene reporters, bacterial sensors
HESSO-ISI Industrial Systems Institute M. Geiser
Microsystems, electronics, optical sensors
ETHZ-MAT Biologically Oriented Materials V. Vogel
Biomaterial, cell biology
CSEM Nanobiotechnology group M. Liley
Surface biochemistry, biomaterials
EPFL-LEPA Electrochemistry and Analytics Lab. H. Girault
Electrochemical sensing, analytical chemistry
EPFL/NE-IMT Sensors and Actuators Laboratory N. de Rooij
Microfluidics, microsensors / P. van der Val
UNIL- IST Institute of Occupational Health M. Riedicker
Health effect of pollution
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SYSTEM INTEGRATIONSYSTEM INTEGRATION
--Systems specificationsSystems specifications
--Electronic platform + demoElectronic platform + demo
--Bioreactor integrationBioreactor integration
--Validation ofValidation ofbioreactor controlbioreactor control
--DemonstrationDemonstration
MICRO-BIOREACTORMICRO-BIOREACTOR
--Bacterial microbioreactorBacterial microbioreactor
--Biomaterials andBiomaterials and3D3Dcultureculture
--Microfluidic platformMicrofluidic platform
--Nutrient and sample supplyNutrient and sample supply
--Integration ofIntegration ofmonitoring sensorsmonitoring sensors
SECONDARY SENSORSSECONDARY SENSORS
--Electrochemical microsensorsElectrochemical microsensors
--Fluorescence/bioluminFluorescence/biolumin. sensors. sensors
--Impedance sensingImpedance sensing
--Sensors validationSensors validation
CELL MODELSCELL MODELS
--SpecificationSpecificationof cell modelsof cell models
--CultureCultureprotocolsprotocols
--AssessmentAssessmentof cell modelsof cell models
--Culture inCulture in microbioreactorsmicrobioreactors
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LMIS
LEPA
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RTGill-W1 cell line (fish)
Cultured between 18-21C
Grow under atmospheric CO2 conditions
Cell line did NOT form tight junctions
Caco-2 cells (intestestine cell line)
Known high TEER values Looking for primary fish cell lines that form tight junctions
Measure Trans Epithelial
Electrical Resistance (TEER)of epithelial cells on ultra-thin
porous silicon nitride
membranes.
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Deposition Points
PBS Inlet
SampleInlet
Outlet
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Cell growth
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Relativeimpedance/%
0.1mM SDS toxicity
Day7
after5
min
after15m
in
after1
.5h
after2
.5h
0
50
100
150
200
250
Relativeimpedance/%
LMIS
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D: Alexa 488
A: Alexa 546
Baneyx, Baugh,Vogel, PNAS 98 (2001)14464 & PNAS 99 (2002)5139.
Smith, Gourdon, Kubow, Little, Vogel, PLOS Biology5 (2007)e268
Biochemical labelingof plasma Fibronectin,
added to cell medium,
harvestedby cells,
incorporatedinto extra-cellular matrix
donors random on lys, acceptors on cys
S SA S S
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Smith, Gourdon, Kubow, Little, Vogel, PLOS Biology, 5 (2007) e268
Little, Smith, Ebneter, Vogel, Matrix Biol. 27 (2008) 451
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CellsCells optical
sensors
nutrient
supply
signal
processing
unit
comm.
unitelectrical
sensors
sampling
data com
microsystem platformbioreactor
power supply
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7/26/2019 Live Sense 2010
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SYSTEM INTEGRATIONSYSTEM INTEGRATION
--Systems specificationsSystems specifications
--Electronic platform + demoElectronic platform + demo
--Bioreactor integrationBioreactor integration
--Validation ofValidation ofbioreactor controlbioreactor control
--DemonstrationDemonstration
MICRO-BIOREACTORMICRO-BIOREACTOR
--Bacterial microbioreactorBacterial microbioreactor
--Biomaterials andBiomaterials and3D3Dcultureculture
--Microfluidic platformMicrofluidic platform
--Nutrient and sample supplyNutrient and sample supply
--Integration ofIntegration ofmonitoring sensorsmonitoring sensors
SECONDARY SENSORSSECONDARY SENSORS
--Electrochemical microsensorsElectrochemical microsensors--Fluorescence/bioluminFluorescence/biolumin. sensors. sensors
--Impedance sensingImpedance sensing
--Sensors validationSensors validation
CELL MODELSCELL MODELS
--SpecificationSpecificationof cell modelsof cell models
--CultureCultureprotocolsprotocols
--AssessmentAssessmentof cell modelsof cell models
--Culture inCulture in microbioreactorsmicrobioreactors
^"Q+ '+":' _
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4/5"6"-'" /-(*'+23 '*::,2+
Lonza Cell culture products access to cells and culture kits
Ayanda Biosystems Bioelectric platform platform, microelectrodes
Debiotech Drug delivery devices micro-pump samples
Novozymes Protein engineering, HTS access to cell based assays
Neroxis/Veolia Chemical microsensors sensor samples
Diagnoswiss Electrochemical immunosensing expertise, sensor samples
Biocartis Multiplex diagnostics protein microcarriers
Trade-4 Telemedecine technical support
+ European centre for validation of alternative methods expertise, standards