Live cell movie analyzer - NanoEnTek Br_Introduction.pdf · Live cell movie analyzer Copyright 2012...

Copyright 2012 @ NanoEnTek Inc. All rights reserved Live cell movie analyzer

Live cell movie analyzer

Understanding cellular growth, behavior and morphology


What is ?

Smart live cell viewer View and capture the live single image inside your cell-culture incubator.

Real time movie recorder Sequential time-lapse images are stored, and can be converted to movie file automatically.

Intelligent confluence analyzer Detect cell confluence and make growth curve using image based analysis.

Real time cell growth curve Applicable long-term monitoring in real time.

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works inside your incubator

Dual system (*Optional) Allow to compare the control and experimental

samples using dual system.

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Features Time-lapse image capture and movie recording

Automated cell confluence detection

10.1” color LCD touch screen

Real-time cell growth curve generation

Focus adjustment through LCD

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Hardware description

Scope unit

Station unit

White LED

Neck

Stage

Focus knob

LCD touch screen

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Software description

Settings menu

Zoom in

Zoom out

Navigation

Focus interface

Exposure

Brightness

Capture

Confluence

Data menu

Monitoring menu

Focusing menu

Focus menu

Scale bar

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Monitoring menu Monitoring menu

Current view

-Total time -Interval -Image -Mode -Elapse time

-File name -Exposure -Brightness -Zoom level

Monitoring setting / Record / Stop

Cell viewer

Cell Growth curve

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Data menu

Cell viewer

Data menu

List / Results

Multi select / Rename

Delete / Save to USB

Screen shot

Cell growth carve

Zoom bar

Current view

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Setting menu

Date & Time

Settings menu

Parameter settings

OS version

Software version

LED power

Disk space

Firmware version

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Software for Dual system

Channel 1: Control sample

Channel 2: Experimental sample

Focusing menu

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Monitoring menu▶ (Setting)

Check the both channels for dual monitoring

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Can monitor the channel 1 & 2 at same time!

Monitoring menu▶ (Recording)

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Can check the data for channel 1 & 2 each, also both of them together in one graph and movie!

Data menu

Channel 1

Channel 2

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PC program description

Review captured image and movie files. Allow to re-make the movie file, also export the law data with spread sheet.

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How to use

Focus with digital focusing knob.

Set the parameters for the cell monitoring. View and transfer the results.

Step 1

Step 2 Step 3

http://youtu.be/mzSr3dWJ1fc

Click the link for GUI operation

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http://youtu.be/mzSr3dWJ1fc


Applications Time-lapse image capture & recording movie Cell-growth images were captured for 60 hours with 10 minute interval in Hep3B cell line.

Automated quantitative cell confluence analysis

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Applications Real-time cell growth curve NIH-3T3 cells growth were observed 50 hours through JuLI™ Br.

All images were recorded every 10 minute and analyzed monolayer confluence.

Cell migration (wound healing) assay

[Wound healing progress images]

A549 cells were incubated for 40 hours after scratch.

JuLI™ Br calculated the confluence with growth of surface

unfarmed automatically.

[Wound healing progress graph]

Wound confluence can be graphed to

quantitatively analysis the recovering

the surface of wound closing.

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Movies for Dual system

[Channel 1] – Experimental sample Morphological changes of SH-SY5Y cells

Incubated with Gold nanorods observed

by using live image movie analyzer, JuLI™ Br

Nanobio research lab, Gachon University (Korea)

Click the image or link for playing

http://youtu.be/jCCFENqhT8M

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[Channel 2] - Control sample SH-SY5Y cells growth observed 24 hours with 5

min interval through Live image movie analyzer,

JuLI™ Br.

Bionano research lab, Gachon University (Korea)


http://youtu.be/9ehAD3pmbjI





[Channel 1] - Control sample For apoptosis assays, control group, NIH-3T3 cells growth

observed 30 hours through Live image movie analyzer, JuLI

Br. This movie was made by 181 images at 10-minute

intervals.

[Channel 2] - Experimental sample For apoptosis assays, experimental group

was treated Staurosporine. NIH-3T3 cells growth

observed 30 hours through live image movie

analyzer, JuLI™ Br.

Research lab, NanoEnTek Inc. (Korea) Research lab, NanoEnTek Inc. (Korea)


http://youtu.be/Xuo8-Xifano

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[Channel 1] - Control sample For apoptosis assays, control group, HeLa cells growth

observed 40 hours with 10 min interval through Live image

movie analyzer, JuLI™ Br.

[Channel 2] - Experimental sample For apoptosis assays, experimental group

was treated Staurosporine. NIH-3T3 cells growth

observed 40 hours through live image movie

analyzer, JuLI™ Br.

Research lab, NanoEnTek Inc. (Korea) Research lab, NanoEnTek Inc. (Korea)


http://youtu.be/ug0R2BJnX2I

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NIH-3T3 cells growth by using JuLI™ Br

NIH-3T3 cells growth observed 64 hours through

live image movie analyzer, JuLI™ Br.

This movie was made by 394 images

at 10-minute intervals.

Research lab, NanoEnTek Inc. (Korea)

http://youtu.be/UmFXLjJx_Jc

Movies

HeLa Group cells growth observed through JuLI™ Br


http://youtu.be/xYYwL24QR_o

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Movies

A549 wound healing assay movie by JuLI™ Br

A549 cells were incubated for 68 hours after scratch.

Live cell movie analyzer, JuLI™ Br calculated

the confluence with growth of surface unfarmed

automatically.

Research lab, NanoEnTek Inc. (Korea)

http://youtu.be/v9xq_GiRXeE

HaCat wound healing assay with Nano ageing 48hrs

For wound healing assays, HaCat cells incubated

with Nano ageing observed 48 hours through Live

image movie analyzer, JuLI Br..

Research lab, Korea University (Korea)

http://youtu.be/XmT0KkIujJU

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http://youtu.be/XmT0KkIujJU


Movies [Control sample]

Fibroblast (FY11) cells growth by using JuLI™ Br


[Experimental sample]

Morphological changes of Fibroblast (FY11) cells

Incubated with Gold nanorods observed

by using live image movie analyzer, JuLI™ Br


http://youtu.be/HN6voPgOWIE

http://youtu.be/y1l8GUKYTM0

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Cat no Device

JULI-BR04 (Single set, 1 Scope & 1 Station)

JULI-BRD04 (Dual set, 2 Scopes & 1 Station)

JULI-BRSC (2nd Scope)

Accessory

JULI-BRTB (XY Stage)

Magnification Objective 4 X and digital zoom (~ 400 X)

Image resolution 2560 X 1920 pixels (5M)

Exported formats

JPEG (image), AVI (movie),

CSV (raw data)

Display 10.1’’ LCD touch screen

Light source White LED

Dimensions & Weight Scope: 300 x 190 x 188 mm, 4 kg

Station: 282 x 285 x 160 mm, 3.2 kg

Storage

320 GB Hard drive

4 GB USB drive

Specification

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Copyright 2012 @ NanoEnTek Inc. All rights reserved ver. 2.5

Live cell movie analyzer

Website

www.nanoentek.com

E-mail

[email protected]

NanoEnTek Inc. (HQ)

12F, Ace High-end Tower, 235-2, Guro3-dong,

Guro-gu, Seoul, 152-740, Korea

Tel : +82-2-6220-7913 / Fax : +82-2-6220-7721

NanoEnTek USA Inc.

5627 Stoneridge Drive Suite 304, Pleasanton,

CA 94588, USA

Tel : +1-925-225-0108, +1-888-988-0108(Toll free)

Fax : +1-925-225-0109

http://www.nanoentek.com/

mailto:[email protected]

Live cell movie analyzer - NanoEnTek Br_Introduction.pdf · Live cell movie analyzer Copyright 2012...

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